CSPI02G27890 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G27890
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionInositol-tetrakisphosphate 1-kinase
LocationChr2: 23878092 .. 23879473 (-)
RNA-Seq ExpressionCSPI02G27890
SyntenyCSPI02G27890
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCATAGCCGCGCTCCACAGTATAAATTACACACAAACTGAGATCATTTTTCTCACCGGAGTTTTTCTTCACATTTTCTCGGCTTCCAAACACCACCTCTGCATCACCAACCATGGCTTCTAATTCTCCTTCTTCAAATCGCCTTCGTGTAGGTTACGCTTTTCCCCCAAACAAAGAGCGCAATGTAATTCGGCCATCTCTAATCGACTACGCCAAGTTACATAGCGTCGACCTCGTTCGTATCGACCTCCAAACGCCGCTCCTCCACCAAGGTCCATTCCACTGCATCATTCACAAACTCTACGACGATGCTTGGGCAGAGAATCTCCAAGAATTCGCCTCCAAGAATCCTGACGTCGTCGTCGTAGATCGGCCAGACCTGATCTCTAAGCTCTACAACAGAGTCTCCATGCTCGATGTAGTGAGCCAGGTGAAGGTTTCGGACTCTGACGTAAAAATCGAGGTGCCGAAACAGATACTTGTGAAACAGGAGGATGAAGCAATCGATTCGATTATGAAATTAGAGTTGAAATTTCCAGTGATTGCTAAGCCGATGGAGTCAGATGGAAGTGCAAAATCGCACGAAATGTCTCTAGTCTTCAACCGACGAGGATTGAAGGATTTGAACAAGCCCGTACTTGTACAGGAATTTGTGAACCACGGCGGAGTAATGTTCAAAATCTACGTTGCAGGAGATCAATCGATGTGTGTTAAGCGGAAATCGCTGCCGGATGTTGAGGAGACAGAGGAAGAACTAGAGAAGAAGACGGAGGGCGCGATGAAGTTTTCGCAGATATCTAGGGCAGAGGAGAAGAGTGAGAAATGCAACGGGGAGGCGAAGAAGGAGGATGAGGAAGAGATTGAAATGCCGCCGGAGAAGATAGTGAGGGAGGTATCGAGAGGGTTGAAGGAAGCGATGGGGATTAGGCTGTTCAATTTTGACATGATAAGAGATAGAAATAATGGAAGGTATTATGTGATAGATATAAATTACTTACCAGGATTTGCAGTTCTGCCGGAGTACGAGCCGTTTCTGACGAAATTCTTCAAGGAAGTTAGGGAGAAGAAGGTGGATGTGGATGAGGTGGCGTCCGCCACTGAAAACGATAAACAAAACCCACGGTGCTGCAGCTGTTTCCATTGAACGTGAAATCCTGTTACAACAATCGAGGAGCATTCAATTCAACCCTTTTTTTTTTTCTTTTTAAAAAACGTTTGTATTCCAGCAATTCTCTTTCATACAAAAAAAAAGAAAAAAAAAAGATACATATAATTTAATAATTTTGCTAATTTGTAAATACCTTTTTTCTTTTTTTGCTATATTTTGAAATGTTCCTCTCTAATTTTTATTTCTTTTTAACTAAAATAGAGGATGAAATGCT

mRNA sequence

CTCATAGCCGCGCTCCACAGTATAAATTACACACAAACTGAGATCATTTTTCTCACCGGAGTTTTTCTTCACATTTTCTCGGCTTCCAAACACCACCTCTGCATCACCAACCATGGCTTCTAATTCTCCTTCTTCAAATCGCCTTCGTGTAGGTTACGCTTTTCCCCCAAACAAAGAGCGCAATGTAATTCGGCCATCTCTAATCGACTACGCCAAGTTACATAGCGTCGACCTCGTTCGTATCGACCTCCAAACGCCGCTCCTCCACCAAGGTCCATTCCACTGCATCATTCACAAACTCTACGACGATGCTTGGGCAGAGAATCTCCAAGAATTCGCCTCCAAGAATCCTGACGTCGTCGTCGTAGATCGGCCAGACCTGATCTCTAAGCTCTACAACAGAGTCTCCATGCTCGATGTAGTGAGCCAGGTGAAGGTTTCGGACTCTGACGTAAAAATCGAGGTGCCGAAACAGATACTTGTGAAACAGGAGGATGAAGCAATCGATTCGATTATGAAATTAGAGTTGAAATTTCCAGTGATTGCTAAGCCGATGGAGTCAGATGGAAGTGCAAAATCGCACGAAATGTCTCTAGTCTTCAACCGACGAGGATTGAAGGATTTGAACAAGCCCGTACTTGTACAGGAATTTGTGAACCACGGCGGAGTAATGTTCAAAATCTACGTTGCAGGAGATCAATCGATGTGTGTTAAGCGGAAATCGCTGCCGGATGTTGAGGAGACAGAGGAAGAACTAGAGAAGAAGACGGAGGGCGCGATGAAGTTTTCGCAGATATCTAGGGCAGAGGAGAAGAGTGAGAAATGCAACGGGGAGGCGAAGAAGGAGGATGAGGAAGAGATTGAAATGCCGCCGGAGAAGATAGTGAGGGAGGTATCGAGAGGGTTGAAGGAAGCGATGGGGATTAGGCTGTTCAATTTTGACATGATAAGAGATAGAAATAATGGAAGGTATTATGTGATAGATATAAATTACTTACCAGGATTTGCAGTTCTGCCGGAGTACGAGCCGTTTCTGACGAAATTCTTCAAGGAAGTTAGGGAGAAGAAGGTGGATGTGGATGAGGTGGCGTCCGCCACTGAAAACGATAAACAAAACCCACGGTGCTGCAGCTGTTTCCATTGAACGTGAAATCCTGTTACAACAATCGAGGAGCATTCAATTCAACCCTTTTTTTTTTTCTTTTTAAAAAACGTTTGTATTCCAGCAATTCTCTTTCATACAAAAAAAAAGAAAAAAAAAAGATACATATAATTTAATAATTTTGCTAATTTGTAAATACCTTTTTTCTTTTTTTGCTATATTTTGAAATGTTCCTCTCTAATTTTTATTTCTTTTTAACTAAAATAGAGGATGAAATGCT

Coding sequence (CDS)

ATGGCTTCTAATTCTCCTTCTTCAAATCGCCTTCGTGTAGGTTACGCTTTTCCCCCAAACAAAGAGCGCAATGTAATTCGGCCATCTCTAATCGACTACGCCAAGTTACATAGCGTCGACCTCGTTCGTATCGACCTCCAAACGCCGCTCCTCCACCAAGGTCCATTCCACTGCATCATTCACAAACTCTACGACGATGCTTGGGCAGAGAATCTCCAAGAATTCGCCTCCAAGAATCCTGACGTCGTCGTCGTAGATCGGCCAGACCTGATCTCTAAGCTCTACAACAGAGTCTCCATGCTCGATGTAGTGAGCCAGGTGAAGGTTTCGGACTCTGACGTAAAAATCGAGGTGCCGAAACAGATACTTGTGAAACAGGAGGATGAAGCAATCGATTCGATTATGAAATTAGAGTTGAAATTTCCAGTGATTGCTAAGCCGATGGAGTCAGATGGAAGTGCAAAATCGCACGAAATGTCTCTAGTCTTCAACCGACGAGGATTGAAGGATTTGAACAAGCCCGTACTTGTACAGGAATTTGTGAACCACGGCGGAGTAATGTTCAAAATCTACGTTGCAGGAGATCAATCGATGTGTGTTAAGCGGAAATCGCTGCCGGATGTTGAGGAGACAGAGGAAGAACTAGAGAAGAAGACGGAGGGCGCGATGAAGTTTTCGCAGATATCTAGGGCAGAGGAGAAGAGTGAGAAATGCAACGGGGAGGCGAAGAAGGAGGATGAGGAAGAGATTGAAATGCCGCCGGAGAAGATAGTGAGGGAGGTATCGAGAGGGTTGAAGGAAGCGATGGGGATTAGGCTGTTCAATTTTGACATGATAAGAGATAGAAATAATGGAAGGTATTATGTGATAGATATAAATTACTTACCAGGATTTGCAGTTCTGCCGGAGTACGAGCCGTTTCTGACGAAATTCTTCAAGGAAGTTAGGGAGAAGAAGGTGGATGTGGATGAGGTGGCGTCCGCCACTGAAAACGATAAACAAAACCCACGGTGCTGCAGCTGTTTCCATTGA

Protein sequence

MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGEAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAVLPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCFH*
Homology
BLAST of CSPI02G27890 vs. ExPASy Swiss-Prot
Match: Q9SBA5 (Inositol-tetrakisphosphate 1-kinase 1 OS=Arabidopsis thaliana OX=3702 GN=ITPK1 PE=1 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 7.1e-75
Identity = 148/313 (47.28%), Postives = 215/313 (68.69%), Query Frame = 0

Query: 10  RLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWA 69
           R  VGYA    K+ + I+PSLI++++   +DLV++D    LL QG   CIIHKLYD  W 
Sbjct: 8   RYLVGYALAAKKQHSFIQPSLIEHSRQRGIDLVKLDPTKSLLEQGKLDCIIHKLYDVYWK 67

Query: 70  ENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVK--VSDSDVKIEVPKQILVKQE 129
           ENL EF  K P V V+D P+ I +L+NRVSML+V++Q++  VSDS+ +  VP+Q++V   
Sbjct: 68  ENLHEFREKCPGVPVIDLPEAIERLHNRVSMLEVITQLRFPVSDSE-RFGVPEQVVVMDS 127

Query: 130 DEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVNHGGVM 189
                     ELKFPVIAKP+++DGSAKSH+M L++++ G+K L  P+++QEFVNHGGV+
Sbjct: 128 SVLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFVNHGGVI 187

Query: 190 FKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGEAKKEDE 249
           FK+YV GD   CVKR+SLPD+    EE    ++G++ FSQIS    + +K     +    
Sbjct: 188 FKVYVVGDHVKCVKRRSLPDI---SEEKIGTSKGSLPFSQISNLTAQEDKNIEYGEDRSL 247

Query: 250 EEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRD-RNNGRYYVIDINYLPGFAVLPEYEP 309
           E++EMPP   + ++++ ++E+MG+ LFNFD+IRD ++  RY +IDINY PG+A +P YEP
Sbjct: 248 EKVEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGYAKMPSYEP 307

Query: 310 FLTKFFKEVREKK 320
            LT+FF ++  KK
Sbjct: 308 VLTEFFWDMVTKK 316

BLAST of CSPI02G27890 vs. ExPASy Swiss-Prot
Match: Q84Y01 (Inositol-tetrakisphosphate 1-kinase 1 OS=Zea mays OX=4577 GN=ITPK1 PE=2 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 4.8e-71
Identity = 150/326 (46.01%), Postives = 207/326 (63.50%), Query Frame = 0

Query: 2   ASNSPSSN-----RLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPF 61
           A+  PSS      R  +GYA  P K+++ I+PSL+  A    +DLV +D   PL  QGPF
Sbjct: 5   AAAEPSSGVTHPPRYVIGYALAPKKQQSFIQPSLVAQAASRGMDLVPVDASQPLAEQGPF 64

Query: 62  HCIIHKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQV-KVSDSDVK 121
           H +IHKLY D W   L  FA+++P V +VD P  I +L+NR+SML VVS++   +D D  
Sbjct: 65  HLLIHKLYGDDWRAQLVAFAARHPAVPIVDPPHAIDRLHNRISMLQVVSELDHAADQDST 124

Query: 122 IEVPKQILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPV 181
             +P Q++V       D  +   L+FP+IAKP+ +DG+AKSH+MSLV++R GL  L  P+
Sbjct: 125 FGIPSQVVVYDAAALADFGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLGKLRPPL 184

Query: 182 LVQEFVNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISR--AEE 241
           ++QEFVNHGGV+FK+YV G    CVKR+SLPDV     E +   +G++ FSQ+S    E 
Sbjct: 185 VLQEFVNHGGVIFKVYVVGGHVTCVKRRSLPDV---SPEDDASAQGSVSFSQVSNLPTER 244

Query: 242 KSEKCNGEAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRD-RNNGRYYVIDI 301
            +E+  GE   ED     +PP   + +++ GL+ A+G++LFNFDMIRD R   RY VIDI
Sbjct: 245 TAEEYYGEKSLED---AVVPPAAFINQIAGGLRRALGLQLFNFDMIRDVRAGDRYLVIDI 304

Query: 302 NYLPGFAVLPEYEPFLTKFFKEVREK 319
           NY PG+A +P YE  LT FF E+  K
Sbjct: 305 NYFPGYAKMPGYETVLTDFFWEMVHK 324

BLAST of CSPI02G27890 vs. ExPASy Swiss-Prot
Match: A0JJZ6 (Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. indica OX=39946 GN=ITPK5 PE=3 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 8.5e-68
Identity = 143/318 (44.97%), Postives = 202/318 (63.52%), Query Frame = 0

Query: 10  RLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWA 69
           R  +GYA  P K+++ I+PSL+  A    +DLV +D   PL  QGPFH +IHKLY + W 
Sbjct: 15  RYLIGYALAPKKQQSFIQPSLVSRAAGRGMDLVPVDPSRPLPEQGPFHLLIHKLYGEEWR 74

Query: 70  ENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKV---SDSDVKIEVPKQILVKQ 129
             L  F++ +P V VVD P  I +L+NR+SML VVS++ V   +       +P Q++V  
Sbjct: 75  GQLDAFSAAHPAVPVVDPPHAIDRLHNRISMLQVVSELDVPLHAHHHHTFGIPSQVVVYD 134

Query: 130 EDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVNHGGV 189
                DS +   L+FP+IAKP+ +DG+AKSH+MSLV++R GL+ L  P+++QEFVNHGGV
Sbjct: 135 AAALSDSGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLRKLRPPLVLQEFVNHGGV 194

Query: 190 MFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRA--EEKSEKCNGEAKK 249
           +FK+YV G    CVKR+SLPDV  ++   +   EG++ FSQ+S    E  +++   + + 
Sbjct: 195 IFKVYVVGAHVTCVKRRSLPDV-SSDVLQDASAEGSLSFSQVSNLPNERTAQEYYDDMRL 254

Query: 250 EDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNG-RYYVIDINYLPGFAVLPE 309
           ED     MPP   + +++  L+ A+G+ LFNFDMIRD   G RY VIDINY PG+A +P 
Sbjct: 255 ED---AIMPPTAFINDIAAALRRALGLHLFNFDMIRDARAGDRYLVIDINYFPGYAKMPG 314

Query: 310 YEPFLTKFFKEVREKKVD 322
           YE  LT FF E+  K  D
Sbjct: 315 YETVLTDFFWEMVHKDDD 328

BLAST of CSPI02G27890 vs. ExPASy Swiss-Prot
Match: Q7XBW0 (Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=ITPK5 PE=2 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 8.5e-68
Identity = 143/318 (44.97%), Postives = 202/318 (63.52%), Query Frame = 0

Query: 10  RLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWA 69
           R  +GYA  P K+++ I+PSL+  A    +DLV +D   PL  QGPFH +IHKLY + W 
Sbjct: 15  RYLIGYALAPKKQQSFIQPSLVSRAAGRGMDLVPVDPSRPLPEQGPFHLLIHKLYGEEWR 74

Query: 70  ENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKV---SDSDVKIEVPKQILVKQ 129
             L  F++ +P V VVD P  I +L+NR+SML VVS++ V   +       +P Q++V  
Sbjct: 75  GQLDAFSAAHPAVPVVDPPHAIDRLHNRISMLQVVSELDVPLHAHHHHTFGIPSQVVVYD 134

Query: 130 EDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVNHGGV 189
                DS +   L+FP+IAKP+ +DG+AKSH+MSLV++R GL+ L  P+++QEFVNHGGV
Sbjct: 135 AAALSDSGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLRKLRPPLVLQEFVNHGGV 194

Query: 190 MFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRA--EEKSEKCNGEAKK 249
           +FK+YV G    CVKR+SLPDV  ++   +   EG++ FSQ+S    E  +++   + + 
Sbjct: 195 IFKVYVVGAHVTCVKRRSLPDV-SSDVLQDASAEGSLSFSQVSNLPNERTAQEYYDDMRL 254

Query: 250 EDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNG-RYYVIDINYLPGFAVLPE 309
           ED     MPP   + +++  L+ A+G+ LFNFDMIRD   G RY VIDINY PG+A +P 
Sbjct: 255 ED---AIMPPTAFINDIAAALRRALGLHLFNFDMIRDARAGDRYLVIDINYFPGYAKMPG 314

Query: 310 YEPFLTKFFKEVREKKVD 322
           YE  LT FF E+  K  D
Sbjct: 315 YETVLTDFFWEMVHKDDD 328

BLAST of CSPI02G27890 vs. ExPASy Swiss-Prot
Match: A2X4M8 (Inositol-tetrakisphosphate 1-kinase 4 OS=Oryza sativa subsp. indica OX=39946 GN=ITPK4 PE=3 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 3.7e-63
Identity = 142/319 (44.51%), Postives = 197/319 (61.76%), Query Frame = 0

Query: 3   SNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHK 62
           S+  SS R  VGYA  P K  +V+RPSL+  A    V LV +D+  PL  QGPF  ++HK
Sbjct: 6   SSPSSSPRYTVGYALLPEKVSSVVRPSLVALAADRGVRLVAVDVSRPLAEQGPFDLLVHK 65

Query: 63  LYDDAWAENLQEFASKNPDV-VVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQ 122
           +YD  W   L+E A+++P V VVVD P  I +L +R +MLDVVS ++       + VP Q
Sbjct: 66  MYDRGWRAQLEELAARHPGVPVVVDSPGAIDRLLDRATMLDVVSGLR-----TPVSVPPQ 125

Query: 123 ILVKQEDEAIDSIM-KLELKFPVIAKPMESDGSAKSHEMSLVFNRRG-LKDLNKPVLVQE 182
           ++V       D ++ +  L+FP+IAKP+  DGSA+SH+M LV+ R G L  L  P+++QE
Sbjct: 126 VVVSDAAADADELLARAALRFPLIAKPLAVDGSAESHDMRLVYRRDGVLPLLRAPLVLQE 185

Query: 183 FVNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCN 242
           FVNHGGV+FK+YV GD++ CV+R SLPDV      L+   E ++ F+ IS         +
Sbjct: 186 FVNHGGVLFKVYVVGDRATCVRRSSLPDV-PARRLLDLDAEPSVPFANISNQPLPPPDDD 245

Query: 243 GEAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDR---NNGRYYVIDINYLP 302
           G A  +D      P    V EV+RGL+  +G+ LFNFDMIR+R   +  RY++IDINY P
Sbjct: 246 GGAADDD-----TPAAGFVDEVARGLRRGLGLHLFNFDMIRERSEEHGDRYFIIDINYFP 305

Query: 303 GFAVLPEYEPFLTKFFKEV 316
           G+A +P YE  LT FF E+
Sbjct: 306 GYAKMPGYEAALTDFFLEM 313

BLAST of CSPI02G27890 vs. ExPASy TrEMBL
Match: A0A0A0LRI5 (Inositol-tetrakisphosphate 1-kinase OS=Cucumis sativus OX=3659 GN=Csa_2G439740 PE=3 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 1.8e-190
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0

Query: 1   MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCII 60
           MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCII
Sbjct: 1   MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCII 60

Query: 61  HKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPK 120
           HKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPK
Sbjct: 61  HKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPK 120

Query: 121 QILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEF 180
           QILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEF
Sbjct: 121 QILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEF 180

Query: 181 VNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNG 240
           VNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNG
Sbjct: 181 VNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNG 240

Query: 241 EAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAV 300
           EAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAV
Sbjct: 241 EAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAV 300

Query: 301 LPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCFH 344
           LPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCFH
Sbjct: 301 LPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCFH 343

BLAST of CSPI02G27890 vs. ExPASy TrEMBL
Match: A0A5A7TY89 (Inositol-tetrakisphosphate 1-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold66G00690 PE=3 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 5.5e-171
Identity = 317/342 (92.69%), Postives = 326/342 (95.32%), Query Frame = 0

Query: 1   MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCII 60
           MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAK H VDLVRIDLQTPLLHQGPFHCII
Sbjct: 1   MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKFHGVDLVRIDLQTPLLHQGPFHCII 60

Query: 61  HKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPK 120
           HKLYDDAWAENLQ+FASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDS V+IEVPK
Sbjct: 61  HKLYDDAWAENLQDFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSGVRIEVPK 120

Query: 121 QILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEF 180
           QILVK+E+EAIDSIM LELKFPVIAKPMESDGSAKSHEMSLVFNRRGLK+LN PVL+QEF
Sbjct: 121 QILVKKEEEAIDSIMNLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKELNTPVLLQEF 180

Query: 181 VNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNG 240
           VNHGGVMFKIYVAGDQS+CVKRKSLPDVE TEEELEKKTEGAMKFSQISRAEEKSEKCNG
Sbjct: 181 VNHGGVMFKIYVAGDQSVCVKRKSLPDVEGTEEELEKKTEGAMKFSQISRAEEKSEKCNG 240

Query: 241 EAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAV 300
           EAKKE E+EIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRD  NG YYVIDINYLPGFAV
Sbjct: 241 EAKKE-EKEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRD-GNGTYYVIDINYLPGFAV 300

Query: 301 LPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCF 343
           LPEYEPFLTKFFKEV EKK  VDEVASA E+DK  PRCCSCF
Sbjct: 301 LPEYEPFLTKFFKEVTEKK--VDEVASAIEDDKAKPRCCSCF 338

BLAST of CSPI02G27890 vs. ExPASy TrEMBL
Match: A0A1S3C9X3 (Inositol-tetrakisphosphate 1-kinase OS=Cucumis melo OX=3656 GN=LOC103498455 PE=3 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 5.5e-171
Identity = 317/342 (92.69%), Postives = 326/342 (95.32%), Query Frame = 0

Query: 1   MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCII 60
           MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAK H VDLVRIDLQTPLLHQGPFHCII
Sbjct: 1   MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKFHGVDLVRIDLQTPLLHQGPFHCII 60

Query: 61  HKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPK 120
           HKLYDDAWAENLQ+FASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDS V+IEVPK
Sbjct: 61  HKLYDDAWAENLQDFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSGVRIEVPK 120

Query: 121 QILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEF 180
           QILVK+E+EAIDSIM LELKFPVIAKPMESDGSAKSHEMSLVFNRRGLK+LN PVL+QEF
Sbjct: 121 QILVKKEEEAIDSIMNLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKELNTPVLLQEF 180

Query: 181 VNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNG 240
           VNHGGVMFKIYVAGDQS+CVKRKSLPDVE TEEELEKKTEGAMKFSQISRAEEKSEKCNG
Sbjct: 181 VNHGGVMFKIYVAGDQSVCVKRKSLPDVEGTEEELEKKTEGAMKFSQISRAEEKSEKCNG 240

Query: 241 EAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAV 300
           EAKKE E+EIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRD  NG YYVIDINYLPGFAV
Sbjct: 241 EAKKE-EKEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRD-GNGTYYVIDINYLPGFAV 300

Query: 301 LPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCF 343
           LPEYEPFLTKFFKEV EKK  VDEVASA E+DK  PRCCSCF
Sbjct: 301 LPEYEPFLTKFFKEVTEKK--VDEVASAIEDDKAKPRCCSCF 338

BLAST of CSPI02G27890 vs. ExPASy TrEMBL
Match: A0A6J1JBH8 (Inositol-tetrakisphosphate 1-kinase OS=Cucurbita maxima OX=3661 GN=LOC111484264 PE=3 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 1.5e-152
Identity = 287/345 (83.19%), Postives = 311/345 (90.14%), Query Frame = 0

Query: 2   ASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIH 61
           +S+S SSNRLRVGYAFPPNKERNVIRPSL+DYAKLH VDLVRID+QTPLLHQGPFHCIIH
Sbjct: 3   SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCIIH 62

Query: 62  KLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQ 121
           KLYDDAWAE+LQ+FASKNPDVVVVDRPDLI++LY+RVSMLDVVSQVKVSDS V+IEVPKQ
Sbjct: 63  KLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVPKQ 122

Query: 122 ILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFV 181
           I++KQED  IDSI  L++KFPVIAKP+ SDGSAKSHEMSLVFNRRGLKDL  PVL+QEFV
Sbjct: 123 IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEFV 182

Query: 182 NHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGE 241
           NHGGVMFK+YVAG++S+CVKRKSLPDV ETEEELEKK +GAMKFSQISRAEEKSEKC+G 
Sbjct: 183 NHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCDGA 242

Query: 242 AKK---EDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGF 301
            KK   E EEEIEMPPEKIVREVS GLKEAMGIRLFNFDMIRD  NG YYVIDINYLPGF
Sbjct: 243 EKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRD-GNGTYYVIDINYLPGF 302

Query: 302 AVLPEYEPFLTKFFKEVRE-KKVDVDEVASATENDKQNPRCCSCF 343
           AVLPEYE FLTKFFKEV E KK  V+E  SA E+ K  PRCC CF
Sbjct: 303 AVLPEYEQFLTKFFKEVAEKKKKKVEEDDSAIEDGKAKPRCC-CF 345

BLAST of CSPI02G27890 vs. ExPASy TrEMBL
Match: A0A6J1FTQ5 (Inositol-tetrakisphosphate 1-kinase OS=Cucurbita moschata OX=3662 GN=LOC111448363 PE=3 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 3.4e-152
Identity = 284/343 (82.80%), Postives = 312/343 (90.96%), Query Frame = 0

Query: 2   ASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIH 61
           +S+S SSNRLRVGYAFPPNKERNVIRPSL+DYAKLH VDLVRIDLQTPLLHQGPFHCIIH
Sbjct: 3   SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCIIH 62

Query: 62  KLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQ 121
           KLYDDAWAE+LQ+FA KNPDVVVVDRPDLI++LY+RVSMLDVVSQVK SDS V+IEVPKQ
Sbjct: 63  KLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVPKQ 122

Query: 122 ILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFV 181
           I++KQED  IDSI  L++KFPVIAKP+ SDGSAKSHEMSLVFNRRGLKDL++PVL+QEFV
Sbjct: 123 IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEFV 182

Query: 182 NHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGE 241
           NHGGVMFK+YVAG++S+CVKRKSLPDV ETEEELEKK +GAMKFSQISRAEEKSEKCNG 
Sbjct: 183 NHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCNG- 242

Query: 242 AKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAVL 301
           A+K++ EEIEMPPEKIVREVS GL+EAMGIRLFNFDMIRD  NG YYVIDINYLPGFAVL
Sbjct: 243 AEKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRD-GNGTYYVIDINYLPGFAVL 302

Query: 302 PEYEPFLTKFFKEVRE--KKVDVDEVASATENDKQNPRCCSCF 343
           PEYE FLTKFFKEV E  KK  V+E  SA E+ K  PRCC CF
Sbjct: 303 PEYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCC-CF 342

BLAST of CSPI02G27890 vs. NCBI nr
Match: XP_004148105.1 (inositol-tetrakisphosphate 1-kinase 1 [Cucumis sativus] >KGN63417.1 hypothetical protein Csa_022560 [Cucumis sativus])

HSP 1 Score: 674.9 bits (1740), Expect = 3.8e-190
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0

Query: 1   MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCII 60
           MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCII
Sbjct: 1   MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCII 60

Query: 61  HKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPK 120
           HKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPK
Sbjct: 61  HKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPK 120

Query: 121 QILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEF 180
           QILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEF
Sbjct: 121 QILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEF 180

Query: 181 VNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNG 240
           VNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNG
Sbjct: 181 VNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNG 240

Query: 241 EAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAV 300
           EAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAV
Sbjct: 241 EAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAV 300

Query: 301 LPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCFH 344
           LPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCFH
Sbjct: 301 LPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCFH 343

BLAST of CSPI02G27890 vs. NCBI nr
Match: XP_008459279.1 (PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis melo] >KAA0047928.1 inositol-tetrakisphosphate 1-kinase 1-like [Cucumis melo var. makuwa] >TYK11159.1 inositol-tetrakisphosphate 1-kinase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 610.1 bits (1572), Expect = 1.1e-170
Identity = 317/342 (92.69%), Postives = 326/342 (95.32%), Query Frame = 0

Query: 1   MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCII 60
           MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAK H VDLVRIDLQTPLLHQGPFHCII
Sbjct: 1   MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKFHGVDLVRIDLQTPLLHQGPFHCII 60

Query: 61  HKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPK 120
           HKLYDDAWAENLQ+FASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDS V+IEVPK
Sbjct: 61  HKLYDDAWAENLQDFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSGVRIEVPK 120

Query: 121 QILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEF 180
           QILVK+E+EAIDSIM LELKFPVIAKPMESDGSAKSHEMSLVFNRRGLK+LN PVL+QEF
Sbjct: 121 QILVKKEEEAIDSIMNLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKELNTPVLLQEF 180

Query: 181 VNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNG 240
           VNHGGVMFKIYVAGDQS+CVKRKSLPDVE TEEELEKKTEGAMKFSQISRAEEKSEKCNG
Sbjct: 181 VNHGGVMFKIYVAGDQSVCVKRKSLPDVEGTEEELEKKTEGAMKFSQISRAEEKSEKCNG 240

Query: 241 EAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAV 300
           EAKKE E+EIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRD  NG YYVIDINYLPGFAV
Sbjct: 241 EAKKE-EKEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRD-GNGTYYVIDINYLPGFAV 300

Query: 301 LPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCF 343
           LPEYEPFLTKFFKEV EKK  VDEVASA E+DK  PRCCSCF
Sbjct: 301 LPEYEPFLTKFFKEVTEKK--VDEVASAIEDDKAKPRCCSCF 338

BLAST of CSPI02G27890 vs. NCBI nr
Match: XP_038900807.1 (inositol-tetrakisphosphate 1-kinase 1-like [Benincasa hispida])

HSP 1 Score: 554.7 bits (1428), Expect = 5.7e-154
Identity = 291/343 (84.84%), Postives = 311/343 (90.67%), Query Frame = 0

Query: 1   MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCII 60
           MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLH VDL+RIDLQTPLLHQGPFHCII
Sbjct: 1   MASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHGVDLIRIDLQTPLLHQGPFHCII 60

Query: 61  HKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPK 120
           HKLYDDAWAENLQ+FASKNPDVVVVD PDLISKLYNRVSMLDVVSQVKVSD+  +I VPK
Sbjct: 61  HKLYDDAWAENLQDFASKNPDVVVVDPPDLISKLYNRVSMLDVVSQVKVSDTGARIAVPK 120

Query: 121 QILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEF 180
           QI++K E+     I  LELKFP+IAKP++SDGSAKSHEMSLVFNRRGLKDL+ PVL+QEF
Sbjct: 121 QIVLKDEE-----IKDLELKFPLIAKPLKSDGSAKSHEMSLVFNRRGLKDLDTPVLLQEF 180

Query: 181 VNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNG 240
           VNHGG MFKIYVAGDQS+CVKRKSLPDV ETEEELEKKTEGAMKFSQISRA EKSEKCNG
Sbjct: 181 VNHGGEMFKIYVAGDQSVCVKRKSLPDVAETEEELEKKTEGAMKFSQISRAVEKSEKCNG 240

Query: 241 EAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFAV 300
           E KKE+E+EIEMPPEK+V EVSRGLKEAMGIRLFN+DMIRD  NG YYVIDINYLPGFAV
Sbjct: 241 EEKKEEEDEIEMPPEKVVTEVSRGLKEAMGIRLFNYDMIRD-GNGMYYVIDINYLPGFAV 300

Query: 301 LPEYEPFLTKFFKEV-REKKVDVDEVASATENDKQNPRCCSCF 343
           LPEYEPFLTKFFKEV  EKKV+  E  SA E++K  PRCC CF
Sbjct: 301 LPEYEPFLTKFFKEVAAEKKVEEAE-DSAIEDEKVKPRCC-CF 335

BLAST of CSPI02G27890 vs. NCBI nr
Match: XP_023512946.1 (inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 551.2 bits (1419), Expect = 6.3e-153
Identity = 287/346 (82.95%), Postives = 312/346 (90.17%), Query Frame = 0

Query: 2   ASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIH 61
           +S+S SSNRLRVGYAFPPNKERNVIRPSL+DYAKLH VDLVRIDLQTPLLHQGPFHCIIH
Sbjct: 5   SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCIIH 64

Query: 62  KLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQ 121
           KLYDDAWAE+LQ+FASKNPDVVVVDRPDLI++LY+RVSMLDVVSQVK SDS V+IEVPKQ
Sbjct: 65  KLYDDAWAEHLQDFASKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVPKQ 124

Query: 122 ILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFV 181
           I++KQED  IDSI  L++KFPVIAKP+ SDGSAKSHEMSLVFNRRGLKDL  PVL+QEFV
Sbjct: 125 IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEFV 184

Query: 182 NHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGE 241
           NHGGVMFK+YVAG++S+CVKRKSLPDV ETEEELEKK +GAMKFSQISRAEEKSEKCNG 
Sbjct: 185 NHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKPDGAMKFSQISRAEEKSEKCNGA 244

Query: 242 AKK---EDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGF 301
            KK   E+EEEIEMPPEKIVREVS GL+EAMGIRLFNFDMIRD  NG YYVIDINYLPGF
Sbjct: 245 EKKAAAEEEEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRD-GNGTYYVIDINYLPGF 304

Query: 302 AVLPEYEPFLTKFFKEV--REKKVDVDEVASATENDKQNPRCCSCF 343
           AVLPEYE FLTKFFKEV  +EKK  V+E  SA E+ K  PRCC CF
Sbjct: 305 AVLPEYEQFLTKFFKEVAEKEKKKKVEEDDSAIEDGKAKPRCC-CF 348

BLAST of CSPI02G27890 vs. NCBI nr
Match: XP_022986561.1 (inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita maxima])

HSP 1 Score: 548.9 bits (1413), Expect = 3.1e-152
Identity = 287/345 (83.19%), Postives = 311/345 (90.14%), Query Frame = 0

Query: 2   ASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIH 61
           +S+S SSNRLRVGYAFPPNKERNVIRPSL+DYAKLH VDLVRID+QTPLLHQGPFHCIIH
Sbjct: 3   SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCIIH 62

Query: 62  KLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQ 121
           KLYDDAWAE+LQ+FASKNPDVVVVDRPDLI++LY+RVSMLDVVSQVKVSDS V+IEVPKQ
Sbjct: 63  KLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVPKQ 122

Query: 122 ILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFV 181
           I++KQED  IDSI  L++KFPVIAKP+ SDGSAKSHEMSLVFNRRGLKDL  PVL+QEFV
Sbjct: 123 IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEFV 182

Query: 182 NHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGE 241
           NHGGVMFK+YVAG++S+CVKRKSLPDV ETEEELEKK +GAMKFSQISRAEEKSEKC+G 
Sbjct: 183 NHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCDGA 242

Query: 242 AKK---EDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGF 301
            KK   E EEEIEMPPEKIVREVS GLKEAMGIRLFNFDMIRD  NG YYVIDINYLPGF
Sbjct: 243 EKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRD-GNGTYYVIDINYLPGF 302

Query: 302 AVLPEYEPFLTKFFKEVRE-KKVDVDEVASATENDKQNPRCCSCF 343
           AVLPEYE FLTKFFKEV E KK  V+E  SA E+ K  PRCC CF
Sbjct: 303 AVLPEYEQFLTKFFKEVAEKKKKKVEEDDSAIEDGKAKPRCC-CF 345

BLAST of CSPI02G27890 vs. TAIR 10
Match: AT5G16760.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 282.3 bits (721), Expect = 5.1e-76
Identity = 148/313 (47.28%), Postives = 215/313 (68.69%), Query Frame = 0

Query: 10  RLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWA 69
           R  VGYA    K+ + I+PSLI++++   +DLV++D    LL QG   CIIHKLYD  W 
Sbjct: 8   RYLVGYALAAKKQHSFIQPSLIEHSRQRGIDLVKLDPTKSLLEQGKLDCIIHKLYDVYWK 67

Query: 70  ENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVK--VSDSDVKIEVPKQILVKQE 129
           ENL EF  K P V V+D P+ I +L+NRVSML+V++Q++  VSDS+ +  VP+Q++V   
Sbjct: 68  ENLHEFREKCPGVPVIDLPEAIERLHNRVSMLEVITQLRFPVSDSE-RFGVPEQVVVMDS 127

Query: 130 DEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVNHGGVM 189
                     ELKFPVIAKP+++DGSAKSH+M L++++ G+K L  P+++QEFVNHGGV+
Sbjct: 128 SVLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFVNHGGVI 187

Query: 190 FKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGEAKKEDE 249
           FK+YV GD   CVKR+SLPD+    EE    ++G++ FSQIS    + +K     +    
Sbjct: 188 FKVYVVGDHVKCVKRRSLPDI---SEEKIGTSKGSLPFSQISNLTAQEDKNIEYGEDRSL 247

Query: 250 EEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRD-RNNGRYYVIDINYLPGFAVLPEYEP 309
           E++EMPP   + ++++ ++E+MG+ LFNFD+IRD ++  RY +IDINY PG+A +P YEP
Sbjct: 248 EKVEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGYAKMPSYEP 307

Query: 310 FLTKFFKEVREKK 320
            LT+FF ++  KK
Sbjct: 308 VLTEFFWDMVTKK 316

BLAST of CSPI02G27890 vs. TAIR 10
Match: AT4G33770.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 232.3 bits (591), Expect = 6.0e-61
Identity = 122/312 (39.10%), Postives = 196/312 (62.82%), Query Frame = 0

Query: 10  RLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWA 69
           +L VGYA    K+++ ++P L   A+   +  V IDL  PL  QGPF  ++HKL    W 
Sbjct: 80  KLVVGYALTSKKKKSFLQPKLELLARRKGIFFVAIDLNRPLSEQGPFDVVLHKLLGKEWE 139

Query: 70  ENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQILVKQEDE 129
           E ++++  K+P+V V+D P  I ++YNR SML  ++ +K+SD    + VPKQ++V ++  
Sbjct: 140 EVIEDYQQKHPEVTVLDPPGSIQRIYNRQSMLQGMADLKLSDCSGSLFVPKQMVVLKDSA 199

Query: 130 A-IDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVNHGGVMF 189
           A  D++++  LKFP++AKP+  DG+AKSH++ L ++RR L +L+ P+++QEFVNHGGVMF
Sbjct: 200 ASADAVVEAGLKFPLVAKPLWIDGTAKSHQLYLAYDRRSLAELDPPLVLQEFVNHGGVMF 259

Query: 190 KIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGEAKKEDEE 249
           K++V GD    ++R SLP+V   E   + K +G  +F ++S A   ++  +      D  
Sbjct: 260 KVFVVGDVIKVMRRFSLPNVSNCE---KAKVDGVFQFPRVSSAAASADNAD-----LDPR 319

Query: 250 EIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGR-YYVIDINYLPGFAVLPEYEPF 309
             E+PP+  +  + + L+  +G+RLFN DMIR+  +   +YVIDINY PG+  LP+YE  
Sbjct: 320 VAELPPKPFLEALVKELRSLLGLRLFNIDMIREHGSKNVFYVIDINYFPGYGKLPDYEQV 379

Query: 310 LTKFFKEVREKK 320
              FF+ + + K
Sbjct: 380 FVDFFQNLAQVK 383

BLAST of CSPI02G27890 vs. TAIR 10
Match: AT4G08170.2 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 224.9 bits (572), Expect = 9.6e-59
Identity = 122/312 (39.10%), Postives = 187/312 (59.94%), Query Frame = 0

Query: 6   PSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIHKLYD 65
           P    + VGYA    K ++ ++P L   A+   +  V ID   PL  QGPF  ++HK   
Sbjct: 36  PVKKSIIVGYALTSKKIKSFLQPKLEGLARNKGILFVAIDQNKPLSEQGPFDIVLHKQIG 95

Query: 66  DAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQILVK 125
             W   L+EF   +PDV V+D PD I  L NR SML  V+ + +SDS+ ++ VPKQ+++K
Sbjct: 96  KEWRRILEEFRLAHPDVTVLDPPDAILHLRNRQSMLQCVADMNLSDSNGRVGVPKQLVIK 155

Query: 126 QEDEAI-DSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVNHG 185
           ++  +I +++    L+ P++AKP+ +DGSAKSHE+SL +++  L  L  P+++QEFVNHG
Sbjct: 156 KDASSIPEAVNNAGLRLPLVAKPLVADGSAKSHELSLAYDQHSLLKLEPPLVLQEFVNHG 215

Query: 186 GVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGEAKK 245
           GV+FK+Y+ G+    V+R SLPDV   E     K+ G  +F ++S A   ++  +     
Sbjct: 216 GVLFKVYIVGEAIRVVRRFSLPDVSRRE---LPKSAGVFRFPRVSCAAASADDAD----- 275

Query: 246 EDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDR-NNGRYYVIDINYLPGFAVLPE 305
            D    E+PP  ++  +++ L+  +G+RLFN D+IR+     R+YVIDINY PG+  +PE
Sbjct: 276 LDPSIAELPPRPLLERLAKELRRGLGLRLFNLDIIREHGTRDRFYVIDINYFPGYGKMPE 335

Query: 306 YEPFLTKFFKEV 316
           YE   T F   V
Sbjct: 336 YEHVFTDFLLSV 339

BLAST of CSPI02G27890 vs. TAIR 10
Match: AT4G33770.2 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 216.5 bits (550), Expect = 3.4e-56
Identity = 113/280 (40.36%), Postives = 179/280 (63.93%), Query Frame = 0

Query: 42  VRIDLQTPLLHQGPFHCIIHKLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSML 101
           V IDL  PL  QGPF  ++HKL    W E ++++  K+P+V V+D P  I ++YNR SML
Sbjct: 19  VAIDLNRPLSEQGPFDVVLHKLLGKEWEEVIEDYQQKHPEVTVLDPPGSIQRIYNRQSML 78

Query: 102 DVVSQVKVSDSDVKIEVPKQILVKQEDEA-IDSIMKLELKFPVIAKPMESDGSAKSHEMS 161
             ++ +K+SD    + VPKQ++V ++  A  D++++  LKFP++AKP+  DG+AKSH++ 
Sbjct: 79  QGMADLKLSDCSGSLFVPKQMVVLKDSAASADAVVEAGLKFPLVAKPLWIDGTAKSHQLY 138

Query: 162 LVFNRRGLKDLNKPVLVQEFVNHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTE 221
           L ++RR L +L+ P+++QEFVNHGGVMFK++V GD    ++R SLP+V   E   + K +
Sbjct: 139 LAYDRRSLAELDPPLVLQEFVNHGGVMFKVFVVGDVIKVMRRFSLPNVSNCE---KAKVD 198

Query: 222 GAMKFSQISRAEEKSEKCNGEAKKEDEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIR 281
           G  +F ++S A   ++  +      D    E+PP+  +  + + L+  +G+RLFN DMIR
Sbjct: 199 GVFQFPRVSSAAASADNAD-----LDPRVAELPPKPFLEALVKELRSLLGLRLFNIDMIR 258

Query: 282 DRNNGR-YYVIDINYLPGFAVLPEYEPFLTKFFKEVREKK 320
           +  +   +YVIDINY PG+  LP+YE     FF+ + + K
Sbjct: 259 EHGSKNVFYVIDINYFPGYGKLPDYEQVFVDFFQNLAQVK 290

BLAST of CSPI02G27890 vs. TAIR 10
Match: AT4G08170.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 189.9 bits (481), Expect = 3.4e-48
Identity = 102/250 (40.80%), Postives = 158/250 (63.20%), Query Frame = 0

Query: 68  WAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQILVKQE 127
           W   L+EF   +PDV V+D PD I  L NR SML  V+ + +SDS+ ++ VPKQ+++K++
Sbjct: 10  WRRILEEFRLAHPDVTVLDPPDAILHLRNRQSMLQCVADMNLSDSNGRVGVPKQLVIKKD 69

Query: 128 DEAI-DSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFVNHGGV 187
             +I +++    L+ P++AKP+ +DGSAKSHE+SL +++  L  L  P+++QEFVNHGGV
Sbjct: 70  ASSIPEAVNNAGLRLPLVAKPLVADGSAKSHELSLAYDQHSLLKLEPPLVLQEFVNHGGV 129

Query: 188 MFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGEAKKED 247
           +FK+Y+ G+    V+R SLPDV   E     K+ G  +F ++S A   ++  +      D
Sbjct: 130 LFKVYIVGEAIRVVRRFSLPDVSRRE---LPKSAGVFRFPRVSCAAASADDAD-----LD 189

Query: 248 EEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDR-NNGRYYVIDINYLPGFAVLPEYE 307
               E+PP  ++  +++ L+  +G+RLFN D+IR+     R+YVIDINY PG+  +PEYE
Sbjct: 190 PSIAELPPRPLLERLAKELRRGLGLRLFNLDIIREHGTRDRFYVIDINYFPGYGKMPEYE 249

Query: 308 PFLTKFFKEV 316
              T F   V
Sbjct: 250 HVFTDFLLSV 251

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SBA57.1e-7547.28Inositol-tetrakisphosphate 1-kinase 1 OS=Arabidopsis thaliana OX=3702 GN=ITPK1 P... [more]
Q84Y014.8e-7146.01Inositol-tetrakisphosphate 1-kinase 1 OS=Zea mays OX=4577 GN=ITPK1 PE=2 SV=1[more]
A0JJZ68.5e-6844.97Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. indica OX=39946 GN=... [more]
Q7XBW08.5e-6844.97Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
A2X4M83.7e-6344.51Inositol-tetrakisphosphate 1-kinase 4 OS=Oryza sativa subsp. indica OX=39946 GN=... [more]
Match NameE-valueIdentityDescription
A0A0A0LRI51.8e-190100.00Inositol-tetrakisphosphate 1-kinase OS=Cucumis sativus OX=3659 GN=Csa_2G439740 P... [more]
A0A5A7TY895.5e-17192.69Inositol-tetrakisphosphate 1-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3C9X35.5e-17192.69Inositol-tetrakisphosphate 1-kinase OS=Cucumis melo OX=3656 GN=LOC103498455 PE=3... [more]
A0A6J1JBH81.5e-15283.19Inositol-tetrakisphosphate 1-kinase OS=Cucurbita maxima OX=3661 GN=LOC111484264 ... [more]
A0A6J1FTQ53.4e-15282.80Inositol-tetrakisphosphate 1-kinase OS=Cucurbita moschata OX=3662 GN=LOC11144836... [more]
Match NameE-valueIdentityDescription
XP_004148105.13.8e-190100.00inositol-tetrakisphosphate 1-kinase 1 [Cucumis sativus] >KGN63417.1 hypothetical... [more]
XP_008459279.11.1e-17092.69PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis melo] >KAA0047928... [more]
XP_038900807.15.7e-15484.84inositol-tetrakisphosphate 1-kinase 1-like [Benincasa hispida][more]
XP_023512946.16.3e-15382.95inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita pepo subsp. pepo][more]
XP_022986561.13.1e-15283.19inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G16760.15.1e-7647.28Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G33770.16.0e-6139.10Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G08170.29.6e-5939.10Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G33770.23.4e-5640.36Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G08170.13.4e-4840.80Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 228..248
NoneNo IPR availableGENE3D3.30.470.20coord: 220..299
e-value: 9.6E-18
score: 66.7
NoneNo IPR availableGENE3D3.40.50.11370coord: 2..114
e-value: 5.1E-23
score: 83.2
NoneNo IPR availableGENE3D3.30.1490.220coord: 121..219
e-value: 6.9E-32
score: 111.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 228..251
NoneNo IPR availablePANTHERPTHR14217:SF24INOSITOL-TETRAKISPHOSPHATE 1-KINASE 1coord: 7..319
NoneNo IPR availableSUPERFAMILY56059Glutathione synthetase ATP-binding domain-likecoord: 88..302
IPR041429Inositol-tetrakisphosphate 1-kinase, N-terminalPFAMPF17927Ins134_P3_kin_Ncoord: 12..91
e-value: 9.2E-24
score: 83.6
IPR008656Inositol-tetrakisphosphate 1-kinasePIRSFPIRSF038186ITPKcoord: 1..328
e-value: 1.2E-109
score: 364.3
IPR008656Inositol-tetrakisphosphate 1-kinasePANTHERPTHR14217INOSITOL-TETRAKISPHOSPHATE 1-KINASEcoord: 7..319
IPR040464Inositol 1,3,4-trisphosphate 5/6-kinase, ATP-grasp domainPFAMPF05770Ins134_P3_kincoord: 112..313
e-value: 8.9E-70
score: 234.3
IPR011761ATP-grasp foldPROSITEPS50975ATP_GRASPcoord: 108..321
score: 18.41328

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G27890.1CSPI02G27890.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0052746 inositol phosphorylation
biological_process GO:0032957 inositol trisphosphate metabolic process
molecular_function GO:0005524 ATP binding
molecular_function GO:0052726 inositol-1,3,4-trisphosphate 5-kinase activity
molecular_function GO:0052725 inositol-1,3,4-trisphosphate 6-kinase activity
molecular_function GO:0047325 inositol tetrakisphosphate 1-kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0046872 metal ion binding