CSPI02G27400 (gene) Cucumber (PI 183967) v1

BLAST of CSPI02G27400 vs. ExPASy TrEMBL
Match: A0A0A0LQT7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G432270 PE=4 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 5.5e-49
Identity = 105/116 (90.52%), Postives = 107/116 (92.24%), Query Frame = 0

Query: 1   MELSLKPVKKRFCQKSESFSELIYLKLIGCEDDDIVCLPLEMSEVLYNIEEFIIEDARKL 60
           MELSLK VKKRFCQKSESFSELI LKLIGCEDDDIVCLPLEMSEVLYNIEEFIIEDARKL
Sbjct: 1   MELSLKSVKKRFCQKSESFSELISLKLIGCEDDDIVCLPLEMSEVLYNIEEFIIEDARKL 60

Query: 61  IQVFETEELSRSNNNDVQRCARLKKLTLWNLPKLMHVWKES------SFDSLENID 111
           IQVFETEELSRSNNNDVQRCARLKKLTLWNLPKLMHVWKES      SFDSLE ++
Sbjct: 61  IQVFETEELSRSNNNDVQRCARLKKLTLWNLPKLMHVWKESSEVTTISFDSLEKVE 116

BLAST of CSPI02G27400 vs. ExPASy TrEMBL
Match: A0A0A0K5R3 (NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G239020 PE=4 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 7.7e-43
Identity = 112/261 (42.91%), Postives = 152/261 (58.24%), Query Frame = 0

Query: 8    VKKRFCQKSESFSELIYLKLIGCEDDDIVCLPLEMSEVLYNIEEFIIEDARKLIQVFETE 67
            VK+RF   S+ F +L  L+L GCED  ++ LP+EM+EVLY+IEE  I    +L+ V    
Sbjct: 1110 VKERFWVMSK-FFKLKSLELFGCEDGKMISLPMEMNEVLYSIEELTIRGCLQLVDVI--- 1169

Query: 68   ELSRSNNNDVQRCARLKKLTLWNLPKLMHVWKESSFDSLENIDIRGCENLKCILASSVTF 127
                 N+  +QRCA LKKL L+NLPKLM+V K  +                    ++ TF
Sbjct: 1170 ----GNDYYIQRCANLKKLKLYNLPKLMYVLKNMN------------------QMTATTF 1229

Query: 128  LNLKYLTVGECSKMMNLFSSTVAENLGNLETTKIYDCREMRCIVAAE-EGEEENGEIVLK 187
              L YL VG C+ M+NLFS +VA+NL NL + +IYDC EMR +VAA+ E EEEN EIV  
Sbjct: 1230 SKLVYLQVGGCNGMINLFSPSVAKNLANLNSIEIYDCGEMRTVVAAKAEEEEENVEIVFS 1289

Query: 188  NLKRIFLHGLPRLACFHNGKCTITFPWLEGFYIYGLYETETFSHGILSFPKLKSMKI-DK 247
             L  +  H L  L CF+ GKCT+ FP L+   I    + + FS+GI + P LK+++I + 
Sbjct: 1290 KLTGMEFHNLAGLECFYPGKCTLEFPLLDTLRISKCDDMKIFSYGITNTPTLKNIEIGEH 1344

Query: 248  REFKIAPGQDINAIIHKRYRI 267
                + P Q IN IIH  + I
Sbjct: 1350 NSLPVLPTQGINDIIHAFFTI 1344

BLAST of CSPI02G27400 vs. ExPASy TrEMBL
Match: A0A0A0LNF6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G432260 PE=4 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 3.3e-38
Identity = 82/100 (82.00%), Postives = 88/100 (88.00%), Query Frame = 0

Query: 141 MMNLFSSTVAENLGNLETTKIYDCREMRCIVAAEEGEEENGEIVLKNLKRIFLHGLPRLA 200
           MMNLFSS+VAENLGNLE+T I++CREMRCIVAAEEGEEENGEIV K+LK I L+GLPRLA
Sbjct: 1   MMNLFSSSVAENLGNLESTVIHECREMRCIVAAEEGEEENGEIVFKHLKEIILYGLPRLA 60

Query: 201 CFHNGKCTITFPWLEGFYIYGLYETETFSHGILSFPKLKS 241
            F NGKCTI FP LE  YIYG YE ETFSHGILSFPKLKS
Sbjct: 61  SFDNGKCTIKFPSLEVLYIYGSYEMETFSHGILSFPKLKS 100

BLAST of CSPI02G27400 vs. ExPASy TrEMBL
Match: A0A1S3CHN4 (disease resistance protein At4g27190-like OS=Cucumis melo OX=3656 GN=LOC103501064 PE=4 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 5.0e-34
Identity = 83/175 (47.43%), Postives = 115/175 (65.71%), Query Frame = 0

Query: 83   LKKLTLWNLPKLMHVWKES-----SFDSLENIDIRGCENLKCILASSVTFLNLKYLTVGE 142
            LK++ +    KL HVWK++     SFDSLE I++  C+NLK +L SS+TFLNLK L + +
Sbjct: 1044 LKRVKIHGCTKLTHVWKDNTKETRSFDSLERIEVEKCKNLKYLLPSSITFLNLKELHIKK 1103

Query: 143  CSKMMNLFSSTVAENLGNLETTKIYDCREMRCIVAAEEGEEENGE-IVLKNLKRIFLHGL 202
            C+ MMNLFSSTV + L NL + K+  C+ MRC+V  E  +EEN E IV K L R+ L  L
Sbjct: 1104 CNGMMNLFSSTVTKKLVNLRSIKVSYCKRMRCMV--EGDQEENDEIIVFKRLSRLELDYL 1163

Query: 203  PRLACFHNGKCTITFPWLEGFYIYGLYETETFSHGILSFPKLKSMKIDKREFKIA 252
            PRL  FH+GKC + FP LE   I    E +TFS+G++  P+L SM ++ + F ++
Sbjct: 1164 PRLDSFHSGKCMLEFPCLESLVIKRCPEMKTFSYGVIIAPRLHSMWMNDKGFGVS 1216

BLAST of CSPI02G27400 vs. ExPASy TrEMBL
Match: A0A0A0LTN4 (NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G433370 PE=4 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 1.8e-31
Identity = 93/259 (35.91%), Postives = 148/259 (57.14%), Query Frame = 0

Query: 18   SFSELIYLKLIGCED-DDIVCLPLEMSEVLYNIEEFIIEDARKLIQVFETEELSRSNNND 77
            SF EL  L ++G  + + +       + VL+++ E  + +   L QV+  + +       
Sbjct: 925  SFPELNDLSIVGGNNLETLWHKNNNPTTVLWSLNELHLLNLPNLKQVWRKDIIK------ 984

Query: 78   VQRCARLKKLTLWNLPKLMHVWKES-----SFDSLENIDIRGCENLKCILASSVTFLNLK 137
            +     LK++ +    KL HVWK++     SFDSLE I++  C+NLK +L SS+ FLNLK
Sbjct: 985  ILTFPSLKRVKIHGCTKLTHVWKDNNKVTRSFDSLERIEVEKCKNLKYLLPSSIAFLNLK 1044

Query: 138  YLTVGECSKMMNLFSSTVAENLGNLETTKIYDCREMRCIVAAEEGEEENGEIV-LKNLKR 197
             L + +C+ M+NLFSSTV + L NL + K+  C+ MRC+V  E  + EN EI+  K L  
Sbjct: 1045 ELHIKKCNGMINLFSSTVTKKLVNLSSIKVSYCKGMRCMV--EVDQAENDEIITFKKLST 1104

Query: 198  IFLHGLPRLACFHNGKCTITFPWLEGFYIYGLYETETFSHGILSFPKLKSMKIDKREFKI 257
            + L  LPRL  F++GKC + FP LE   I    E +TFS+G++  P+L+++ ++ +EF +
Sbjct: 1105 LELDYLPRLDSFYSGKCMLEFPCLESLVIKRCPEMKTFSYGVIIAPRLQTLWMNDKEFGV 1164

Query: 258  -APGQDINAIIHKRYRIII 269
             +P   IN  I    R ++
Sbjct: 1165 SSPACGINETIQNFPRRVV 1175

BLAST of CSPI02G27400 vs. NCBI nr
Match: KAE8652339.1 (hypothetical protein Csa_022544 [Cucumis sativus])

HSP 1 Score: 383.3 bits (983), Expect = 1.8e-102
Identity = 195/221 (88.24%), Postives = 202/221 (91.40%), Query Frame = 0

Query: 1   MELSLKPVKKRFCQKSESFSELIYLKLIGCEDDDIVCLPLEMSEVLYNIEEFIIEDARKL 60
           MELSLK VKKRFCQKSESFSELI LKLIGCEDDDIVCLPLEMSEVLYNIEEFIIEDARKL
Sbjct: 4   MELSLKSVKKRFCQKSESFSELISLKLIGCEDDDIVCLPLEMSEVLYNIEEFIIEDARKL 63

Query: 61  IQVFETEELSRSNNNDVQRCARLKKLTLWNLPKLMHVWKES------SFDSLENIDIRGC 120
           IQVFETEELSRSNNNDVQRCARLKKLTLWNLPKLMHVWKES      SFDSLENIDIRGC
Sbjct: 64  IQVFETEELSRSNNNDVQRCARLKKLTLWNLPKLMHVWKESSEVTTISFDSLENIDIRGC 123

Query: 121 ENLKCILASSVTFLNLKYLTVGECSKMMNLFSSTVAENLGNLETTKIYDCREMRCIVAAE 180
           ENLKCIL SSVTFLNLKYL VG C+KMMNLFSS+VAENLGNLE+T I++CREMRCIVAAE
Sbjct: 124 ENLKCILPSSVTFLNLKYLRVGGCNKMMNLFSSSVAENLGNLESTVIHECREMRCIVAAE 183

Query: 181 EGEEENGEIVLKNLKRIFLHGLPRLACFHNGKCTITFPWLE 216
           EGEEENGEIV K+LK I L+GLPRLA F NGKCTI FP LE
Sbjct: 184 EGEEENGEIVFKHLKEIILYGLPRLASFDNGKCTIKFPSLE 224

BLAST of CSPI02G27400 vs. NCBI nr
Match: XP_011659043.1 (probable disease resistance protein At4g27220 isoform X1 [Cucumis sativus])

HSP 1 Score: 362.1 bits (928), Expect = 4.2e-96
Identity = 189/271 (69.74%), Postives = 211/271 (77.86%), Query Frame = 0

Query: 1    MELSLKPVKKRFCQKSESFSELIYLKLIGCEDDDIVCLPLEMSEVLYNIEEFIIEDARKL 60
            ++LSLK VKK F QK ESFSEL  L+L GCEDDDIVCLPLEM EVLYN E+  I++  +L
Sbjct: 1355 LKLSLKSVKKGFRQKPESFSELKSLELFGCEDDDIVCLPLEMKEVLYNTEKIEIKNGHQL 1414

Query: 61   IQVFETEELSRSNNNDVQRCARLKKLTLWNLPKLMHVWKES------SFDSLENIDIRGC 120
            +QVFE EELSR NN+DVQRC +LK LTL NLPKLMHVWKES      SFDSLE I+IR C
Sbjct: 1415 VQVFENEELSRRNNDDVQRCGKLKNLTLSNLPKLMHVWKESSEVTTISFDSLEKINIRKC 1474

Query: 121  ENLKCILASSVTFLNLKYLTVGECSKMMNLFSSTVAENLGNLETTKIYDCREMRCIVAAE 180
            ENLKCIL SSVTFLNLK+L + EC+KMMNLFSS+VAE L NLE+  +  C EMRCIV  E
Sbjct: 1475 ENLKCILPSSVTFLNLKFLWIRECNKMMNLFSSSVAETLRNLESIDVSHCSEMRCIVTPE 1534

Query: 181  EGEEENGEIVLKNLKRIFLHGLPRLACFHNGKCTITFPWLEGFYI-YGLYETETFSHGIL 240
             GEEENGEIV KNLK I L GLPRLACFHNGKC I FP LE   I    YE ETFSHGIL
Sbjct: 1535 GGEEENGEIVFKNLKSIILFGLPRLACFHNGKCMIKFPSLEILNIGCRRYEMETFSHGIL 1594

Query: 241  SFPKLKSMKIDKREFKIAPGQDINAIIHKRY 265
            SFP LKSM+I++ EFKI+PGQDIN II   +
Sbjct: 1595 SFPTLKSMEIEECEFKISPGQDINVIIRSHF 1625

BLAST of CSPI02G27400 vs. NCBI nr
Match: XP_031744707.1 (uncharacterized protein LOC101213664 isoform X3 [Cucumis sativus] >KAE8646109.1 hypothetical protein Csa_016362 [Cucumis sativus])

HSP 1 Score: 362.1 bits (928), Expect = 4.2e-96
Identity = 189/271 (69.74%), Postives = 211/271 (77.86%), Query Frame = 0

Query: 1    MELSLKPVKKRFCQKSESFSELIYLKLIGCEDDDIVCLPLEMSEVLYNIEEFIIEDARKL 60
            ++LSLK VKK F QK ESFSEL  L+L GCEDDDIVCLPLEM EVLYN E+  I++  +L
Sbjct: 1141 LKLSLKSVKKGFRQKPESFSELKSLELFGCEDDDIVCLPLEMKEVLYNTEKIEIKNGHQL 1200

Query: 61   IQVFETEELSRSNNNDVQRCARLKKLTLWNLPKLMHVWKES------SFDSLENIDIRGC 120
            +QVFE EELSR NN+DVQRC +LK LTL NLPKLMHVWKES      SFDSLE I+IR C
Sbjct: 1201 VQVFENEELSRRNNDDVQRCGKLKNLTLSNLPKLMHVWKESSEVTTISFDSLEKINIRKC 1260

Query: 121  ENLKCILASSVTFLNLKYLTVGECSKMMNLFSSTVAENLGNLETTKIYDCREMRCIVAAE 180
            ENLKCIL SSVTFLNLK+L + EC+KMMNLFSS+VAE L NLE+  +  C EMRCIV  E
Sbjct: 1261 ENLKCILPSSVTFLNLKFLWIRECNKMMNLFSSSVAETLRNLESIDVSHCSEMRCIVTPE 1320

Query: 181  EGEEENGEIVLKNLKRIFLHGLPRLACFHNGKCTITFPWLEGFYI-YGLYETETFSHGIL 240
             GEEENGEIV KNLK I L GLPRLACFHNGKC I FP LE   I    YE ETFSHGIL
Sbjct: 1321 GGEEENGEIVFKNLKSIILFGLPRLACFHNGKCMIKFPSLEILNIGCRRYEMETFSHGIL 1380

Query: 241  SFPKLKSMKIDKREFKIAPGQDINAIIHKRY 265
            SFP LKSM+I++ EFKI+PGQDIN II   +
Sbjct: 1381 SFPTLKSMEIEECEFKISPGQDINVIIRSHF 1411

BLAST of CSPI02G27400 vs. NCBI nr
Match: XP_031744706.1 (probable disease resistance protein At4g27220 isoform X2 [Cucumis sativus])

HSP 1 Score: 362.1 bits (928), Expect = 4.2e-96
Identity = 189/271 (69.74%), Postives = 211/271 (77.86%), Query Frame = 0

Query: 1    MELSLKPVKKRFCQKSESFSELIYLKLIGCEDDDIVCLPLEMSEVLYNIEEFIIEDARKL 60
            ++LSLK VKK F QK ESFSEL  L+L GCEDDDIVCLPLEM EVLYN E+  I++  +L
Sbjct: 1189 LKLSLKSVKKGFRQKPESFSELKSLELFGCEDDDIVCLPLEMKEVLYNTEKIEIKNGHQL 1248

Query: 61   IQVFETEELSRSNNNDVQRCARLKKLTLWNLPKLMHVWKES------SFDSLENIDIRGC 120
            +QVFE EELSR NN+DVQRC +LK LTL NLPKLMHVWKES      SFDSLE I+IR C
Sbjct: 1249 VQVFENEELSRRNNDDVQRCGKLKNLTLSNLPKLMHVWKESSEVTTISFDSLEKINIRKC 1308

Query: 121  ENLKCILASSVTFLNLKYLTVGECSKMMNLFSSTVAENLGNLETTKIYDCREMRCIVAAE 180
            ENLKCIL SSVTFLNLK+L + EC+KMMNLFSS+VAE L NLE+  +  C EMRCIV  E
Sbjct: 1309 ENLKCILPSSVTFLNLKFLWIRECNKMMNLFSSSVAETLRNLESIDVSHCSEMRCIVTPE 1368

Query: 181  EGEEENGEIVLKNLKRIFLHGLPRLACFHNGKCTITFPWLEGFYI-YGLYETETFSHGIL 240
             GEEENGEIV KNLK I L GLPRLACFHNGKC I FP LE   I    YE ETFSHGIL
Sbjct: 1369 GGEEENGEIVFKNLKSIILFGLPRLACFHNGKCMIKFPSLEILNIGCRRYEMETFSHGIL 1428

Query: 241  SFPKLKSMKIDKREFKIAPGQDINAIIHKRY 265
            SFP LKSM+I++ EFKI+PGQDIN II   +
Sbjct: 1429 SFPTLKSMEIEECEFKISPGQDINVIIRSHF 1459

BLAST of CSPI02G27400 vs. NCBI nr
Match: XP_038900321.1 (uncharacterized protein LOC120087577 [Benincasa hispida])

HSP 1 Score: 270.0 bits (689), Expect = 2.2e-68
Identity = 146/266 (54.89%), Postives = 187/266 (70.30%), Query Frame = 0

Query: 1   MELSLKPVKKRFCQKSESFSELIYLKLIGCEDDDIVCLPLEMSEVLYNIEEFIIEDARKL 60
           +EL LK VK+ F  K  SF +L  L+LIGCEDDD+VCLP E+SEVLYNI  F IE+A +L
Sbjct: 217 VELRLKRVKRGFWHKLNSFPQLECLRLIGCEDDDMVCLPSEVSEVLYNIRTFKIENALQL 276

Query: 61  IQVFETEELSRSNNNDVQRCARLKKLTLWNLPKLMHVWKES------SFDSLENIDIRGC 120
           + VFE E L  + NN VQRCA+LK++ LW+LPKL HVWKES      S DSLE++++R C
Sbjct: 277 VHVFENEVL--TTNNHVQRCAKLKEIYLWDLPKLTHVWKESSEVITVSLDSLEHMEVRRC 336

Query: 121 ENLKCILASSVTFLNLKYLTVGECSKMMNLFSSTVAENLGNLETTKIYDCREMRCIVAAE 180
           ENLKCI+ SS +FLNL+ L V  C++M+NLFSS+VA NL NLE  +I DC  M  IV A 
Sbjct: 337 ENLKCIVPSSFSFLNLRTLIVNNCNRMVNLFSSSVANNLENLEKIEISDCTGMTSIVIA- 396

Query: 181 EGEEENGEIVLKNLKRIFLHGLPRLACFHNGKCTITFPWLEGFYIYGLYETETFSHGILS 240
             +EENGEI+ K L+ + L  LPRL  F++GKC + FP LE   I+   + ETFS G++S
Sbjct: 397 --DEENGEIIFKKLRCLGLDNLPRLFSFYSGKCMLKFPSLETLDIFQCNQMETFSVGMVS 456

Query: 241 FPKLKSMKIDKREFKIAPGQDINAII 261
              L+SM I + EF I+P +DIN II
Sbjct: 457 TASLESMTIGESEFIISPTRDINVII 477

BLAST of CSPI02G27400 vs. TAIR 10
Match: AT5G46450.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 49.3 bits (116), Expect = 5.7e-06
Identity = 47/150 (31.33%), Postives = 70/150 (46.67%), Query Frame = 0

Query: 93  KLMHVWK-ESSFDSLENIDIRGCENLKCILASSVTFLNLKYLTVGECSKMMNLFSSTVAE 152
           KL  +W    S   L N+D+RG ENLK I   S+   NLK L V  C+ ++ L SST+ +
Sbjct: 616 KLEKLWDGVHSLTGLRNMDLRGSENLKEIPDLSLA-TNLKKLDVSNCTSLVEL-SSTI-Q 675

Query: 153 NLGNLETTKIYDCREMRCIVAAEEGEEENGEIVLKNLKRIFLHGLPRLACFHNGKCTIT- 212
           NL  LE  ++  C  +  +            I L++L  + L+G  +L  F +   TI+ 
Sbjct: 676 NLNQLEELQMERCENLENLPIG---------INLESLYCLNLNGCSKLRSFPDISTTISE 735

Query: 213 ----------FP---WLEGFYIYGLYETET 228
                     FP    LE  Y  GLY+ ++
Sbjct: 736 LYLSETAIEEFPTELHLENLYYLGLYDMKS 753

BLAST of CSPI02G27400 vs. TAIR 10
Match: AT1G69550.1 (disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 45.1 bits (105), Expect = 1.1e-04
Identity = 52/200 (26.00%), Postives = 88/200 (44.00%), Query Frame = 0

Query: 18   SFSELIYLK---LIGCEDDDIVCLPLEMSEVLYNIEEFIIEDARKLIQV-FETEELSRSN 77
            S   LI LK   L GC    +V LPL +   L N++E  + +   L+++      L    
Sbjct: 879  SIGNLINLKKLDLSGC--SSLVELPLSIGN-LINLQELYLSECSSLVELPSSIGNLINLK 938

Query: 78   NNDVQRCARLKKL--TLWNLPKLMHVWKE------------SSFDSLENIDIRGCENLKC 137
              ++  C+ L +L  ++ NL  L  ++               +  +L+ +D+ GC +L  
Sbjct: 939  TLNLSECSSLVELPSSIGNLINLQELYLSECSSLVELPSSIGNLINLKKLDLSGCSSLVE 998

Query: 138  ILASSVTFLNLKYLTVGECSKMMNLFSSTVAENLGNLETTKIYDCREMRCIVAAEEGEEE 197
            +  S    +NLK L + ECS ++ L SS    NL NL+   + +C  +       E    
Sbjct: 999  LPLSIGNLINLKTLNLSECSSLVELPSS--IGNLINLQELYLSECSSL------VELPSS 1058

Query: 198  NGEIVLKNLKRIFLHGLPRL 200
             G ++  NLK++ L G   L
Sbjct: 1059 IGNLI--NLKKLDLSGCSSL 1065

BLAST of CSPI02G27400 vs. TAIR 10
Match: AT5G38850.1 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 43.1 bits (100), Expect = 4.1e-04
Identity = 33/95 (34.74%), Postives = 52/95 (54.74%), Query Frame = 0

Query: 78  QRCARLKKLTL---WNLPKLMHVWKESSFDSLENIDIRGCENLKCILASSVTFLN-LKYL 137
           Q  A LKK++L   W L KL  +   S+  +LE +D+R C+NL   L SS ++L+ LKYL
Sbjct: 611 QPLANLKKMSLSSSWYLKKLPDL---SNATNLEELDLRACQNL-VELPSSFSYLHKLKYL 670

Query: 138 TVGECSKMMNLFSSTVAENLGNLETTKIYDCREMR 169
            +  C ++  +       NL +LE   +Y C  ++
Sbjct: 671 NMMGCRRLKEVPPHI---NLKSLELVNMYGCSRLK 698