CSPI02G27020 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G27020
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description(-)-germacrene D synthase-like
LocationChr2: 23064212 .. 23068690 (-)
RNA-Seq ExpressionCSPI02G27020
SyntenyCSPI02G27020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAACCTGACGTTAAAGTTCAATAACTTTCCCACTAGCTACACCCAAGAATCATCATCCAAATCCTCATTAAACGATTCTTATAAAGCTGGTGAGGAAGGATCTTATACATATTCTCATAAAAGGAAAAGAGGTCGAGAGAAAAAAAGAAAAATGTCATTTCAAATAACTCCATCAGAATCCGAGGCAGCAAATAAACTTACTTTAAGCGACAATGATGTTCTTCGTCGCATTGAATACCATGAACCAAGTGCTTGGGGAGATTATTTCCTTTCCAATATTTCCAATCTTTCCATTCTTTTGGTATATTTTTGTCTTTTAATTACTGTTATTGTTTCAAATGCAATTTCTGTATTTATATGTTACATAAAGGTTATGAAAATTCAGGAAGAAGATTCAGTCCTGAAAAAACTTGAAGAACTTAAAGAAGAAGCGAGAAGCATGTTTGTAGCTGCTGAAAAGCATTCAGAAAAATTGAGTTTGATTGATTCACTCCAATGTTTAGGTTTGTCATACCACTTTGAAGATGAAATTAACAAAATACTTGATCAAATTCAAAATTCTGCCCATGTTGATGAAGAAGATGTGGAGGATCTTTACATTGTGGCTCTTAAATTCCGATTGCTTAGGCAACGAGGTTTCTTTGTTTCTTGTGGTAAGTCTCTCTCATTCAATTAATTTTACCATTTATTGATGCTAGTAAAAGATTATGACATTTAAACTCAATTATTATTTTGGGTCACATGCCAAAATCAAAAACAAGTTATAATAAGGGATTAAATTCAAATTAATTAAGTATATAGCACAATTTTAAAAATTTTATAAATATAACAAAATTTGTCAAATTCTATCAATGATATAAGTTTATCACTGATAGACCATGTTGCAAATATTGGTCTATCACTGATATACCATATGAAATCTATCAGCGATACAAGTGTATTAGTGATAGTTTTGCTATATTTACAATTTTTTAAAAATGTTACTATATCCTTAATTATTATTGCTAAAATAGTCATCCATTGTAATTTCCCTTATAATAATTAAGTCCATAGCACACACACTTTATTATTTGTGAAAATGGCAAAAACAAAGCCTAACCCACACATCAAGAGGTAAAATGTCACAAATTCCCTTGTTACTAATATACGTTTGATACACTTAATACCCTTTGATACACATAATACTTTTGGATACACCTCATACATTATATTTGATTGATACACTTGATGTACCTGATACTCGTTAGTACACTCGATAACTTTTGATACACACTTGAAACATCTTGATACATATTATAATACTCCTCGTTACACTTGATTCTTCTTGATACACTTGATACTCCTTAGGCCTTGATACAGTACTTGATTAGTTTGATACACTTAAAGACTTCACTTATAAGTTTGATACACCGCTAATAAACTTGCTATGTTTCATTGGTACACTCAACAAATTTGATATGTTTGATGTACATGATATGTTTGATACACTCGATGTACTGCAGATACACTTGGTATTCTTCACTTCTACATTTGATTGATTAGATACACTTGATATGCTTGAAGTCCTACTTATATACTTGATATACTATCAATATACGCTTATAAACTTAATTCATATACTTGTTAATGATTCATTTTACTGATTCACTTTAACAATATATTGTTGATATACTTGATAATTATACGTTGAGTTACATCATTCATATACTTAATTATACTCTAATGAACTGCATAAAATTTAGAAAAACGAAAATCACGTTAACCAACTAATACATATAATATAAGTATATCACAACACTAATATACAAAACTCAGACAAAATAAAATCAAAACAAAATCAATGAAAAATAAATAAAATAAAATCAACTCTAAATACAAATCGAAGAAAGTAAAAAAAAATCACAAATGAAGTTAAATTATTCCGATCAACAACCATTATTTTTTATATGACAATTATTGTACTATTGATATTCTAAAATCAAGATTAAGACATAAAAAATAAATAATTAGAGAATAAATATATAATGAGTAGAGCATTAAAAAGAGGAAGAGAAATAAGTTATTAAAATGTAATTTAAGAATAATAAAAAATTTAAGATGAATATTTTTATAAGTGAAAATTTTGTCATATTTTACGTCCGGTCTCGATTTGCTATATTTTTTAGACTTGCAGATAATGTACCAACATCATTCCTTTTAAATTAACAAAATATTGTTAATGATAAGAAAATATGGTTAAGAAAGATTTTTAAAAAATTATATAGCTAATATCTATACCAAGGTGCAATATTCTTTTTCATTGTTTAATCATAGAAAACCTTATATAATTTTGAATAAAATTTTTAAACCCTCTCTGTGACGCCACTTCCTTCAAAATTAATATTTTTTTTATTTCGACTTGTTCAACTTTTCAAAGTGCATGGATTTATATTATTTCCAATAATTTTCTAAAAGAGGCTGTGACATAAACATTGTAAATTTCAATAGAGATGTTCAAAAAGTTTACAAGTGAAAGTGGAGATTTCAAAGAGTCAATTACAAAAGATGAAAGAGGTTTATTAAGCTTGTATGAGGCATCTCACTTGAGAATGAAGGGAGAAAATATAATGGATGAAGCATTAGAATTCACATCAACTCAACTTGAAGCAATGGCAATGGATTCCACTTCTCCATTCTCAGAAGAAGCTAAATATGCATTAAAGTGGCCAATCTATAAAACTTTGCCAAGGTTCATGACTAGGAACTATATCTCTCTCTATCGCAACAATCCATTGAAGAATAGTAATATTTTGCTCACTTTTGCAAAGTTAGACTACAATTCTCTTCAAAAACTCTACCAAAGAGAACTTGGTGAACTTTCAAGGTATCTTTTTTCAATAAAAACTTCCTATACCATCTTTGAAAATTTTCTTGTTGTTAATGTTTTTCTAATTATGAATGTATATGTACATATATAGGTGGTGGAATGATTTGAAGTTAAAAGAGAAATTACCTTTTGCAAGAGATAGAGTGGTGGAGAGCTATATTTGGGCATTGGGAATATACTATGAACCAAAGTACTCTAGTGCTAGAACAATAGTTGCCAAAATTATTGCTATCATAACAGTTATTGATGATATGTATGATTGTTATGGCACACTTGAAGAACTTCAACTATTCACCAAAGCAATTGAAAGGTTTGGCTCTTCAAAACTCTTAGTTTTAGCATTTGTTAAAATTATATATTAATTAAAGTAATCAAATTTACCTTAACTAATATTGATTTTCCATCTAATATTTTGATTTTTTTTTTATTAAATATAAAGATGGGATTTGAATTGCATTGAGGAGCTCCCAAACTACATGAAAGTTTTGTATGAAGCTATACTTGAATTTTATGAAGGGAGTATAAAAGATATGTGCATGGACAACAATATTCCATATGCATTTGATTATGCAAAAGAAGGGGTAAGAGATCTCTATGACATTGTAATAATGTGGTTTCATCAAAAGAGTACCCACTTTCTAATACGTTTTAGGGTTTTGAGGACAGATAATAAGGCAATGTAAACTTTATATAGTGGAAGCTAAATGGTTCAATGAAGACTATGTGCCAACAATAGAAGAGTACATGAAAATTAGTGCCACTTCCATTGGCGTGTATGCTGTGGCTTCAATTGCCTTCCTATCTTTAGGAAATATTGTTTCAGAAGAGGTTTTCCAATGGGTTCAAGGTAACCCTATGCTTCATCAAGCAAGTGAAGCTGCTAGTAGACTTGTGAATGACATAGTTTCCCACAAGGTACGTACAGTAGATGAAAGTTGAATTGTTAGCTAACTTAAAAAATAAAAAGAAAATTTGAAAATATATGTAAAATAGTAGATAACTAATAAAAAAATTAGTGACTGAATGAACAAAAAAAACTAATAACCAAATGGTTGTCAAGGGGTGAGAGCGAAGGTCTTAGATCTTGGTTTTTCAGTTTTGAAGATTAAGTCTACATAAACAACATCGATCCACCTTTAAATTTCTTCTTTACGAAACAAATGGCTTAATAAAGAATAATTATCATGTGTCTACATATTTTTAGTTTGGAGAAGAGAGGTGCCATGTTACTTGTTCGATTGAGTGCTACATGGAACAATATGGAGTATCAAAAGAGATAGCCGTAGCTGAACTGAAAAAGCAAGTGGGTGAAGCATGGAAAGAAATAATAGAAGATTACATAAAACGTGGCAAGTTTCCAAGTGTGATTCATGATTATGGACCCAATTTTGCTCGAGTCACAGACTTCTATTATAAGGAACGAGATGGTTTCACTTTTGCTAATACAGAAACTAAACATCTTATCGCCTTATTGCTTACAGAGCCTGTGCCAATTTGAACATATCTATAATCTACTTATTTAAACTTGTAATGCCTTTAATAAAAGTTGTAAGAATGCTTCGCAGTTTGAACTTAATATGTTTTCATGTACTTTGTTATGTGTGTTTTATTAA

mRNA sequence

CAACCTGACGTTAAAGTTCAATAACTTTCCCACTAGCTACACCCAAGAATCATCATCCAAATCCTCATTAAACGATTCTTATAAAGCTGGTGAGGAAGGATCTTATACATATTCTCATAAAAGGAAAAGAGGTCGAGAGAAAAAAAGAAAAATGTCATTTCAAATAACTCCATCAGAATCCGAGGCAGCAAATAAACTTACTTTAAGCGACAATGATGTTCTTCGTCGCATTGAATACCATGAACCAAGTGCTTGGGGAGATTATTTCCTTTCCAATATTTCCAATCTTTCCATTCTTTTGGAAGAAGATTCAGTCCTGAAAAAACTTGAAGAACTTAAAGAAGAAGCGAGAAGCATGTTTGTAGCTGCTGAAAAGCATTCAGAAAAATTGAGTTTGATTGATTCACTCCAATGTTTAGGTTTGTCATACCACTTTGAAGATGAAATTAACAAAATACTTGATCAAATTCAAAATTCTGCCCATGTTGATGAAGAAGATGTGGAGGATCTTTACATTGTGGCTCTTAAATTCCGATTGCTTAGGCAACGAGGTTTCTTTGTTTCTTGTGAGATGTTCAAAAAGTTTACAAGTGAAAGTGGAGATTTCAAAGAGTCAATTACAAAAGATGAAAGAGGTTTATTAAGCTTGTATGAGGCATCTCACTTGAGAATGAAGGGAGAAAATATAATGGATGAAGCATTAGAATTCACATCAACTCAACTTGAAGCAATGGCAATGGATTCCACTTCTCCATTCTCAGAAGAAGCTAAATATGCATTAAAGTGGCCAATCTATAAAACTTTGCCAAGGTTCATGACTAGGAACTATATCTCTCTCTATCGCAACAATCCATTGAAGAATAGTAATATTTTGCTCACTTTTGCAAAGTTAGACTACAATTCTCTTCAAAAACTCTACCAAAGAGAACTTGGTGAACTTTCAAGGTGGTGGAATGATTTGAAGTTAAAAGAGAAATTACCTTTTGCAAGAGATAGAGTGGTGGAGAGCTATATTTGGGCATTGGGAATATACTATGAACCAAAGTACTCTAGTGCTAGAACAATAGTTGCCAAAATTATTGCTATCATAACAGTTATTGATGATATGTATGATTGTTATGGCACACTTGAAGAACTTCAACTATTCACCAAAGCAATTGAAAGATGGGATTTGAATTGCATTGAGGAGCTCCCAAACTACATGAAAGTTTTGTATGAAGCTATACTTGAATTTTATGAAGGGAGTATAAAAGATATGTGCATGGACAACAATATTCCATATGCATTTGATTATGCAAAAGAAGGGATAATAAGGCAATGTAAACTTTATATAGTGGAAGCTAAATGGTTCAATGAAGACTATGTGCCAACAATAGAAGAGTACATGAAAATTAGTGCCACTTCCATTGGCGTGTATGCTGTGGCTTCAATTGCCTTCCTATCTTTAGGAAATATTGTTTCAGAAGAGGTTTTCCAATGGGTTCAAGGTAACCCTATGCTTCATCAAGCAAGTGAAGCTGCTAGTAGACTTGTGAATGACATAGTTTCCCACAAGTTTGGAGAAGAGAGGTGCCATGTTACTTGTTCGATTGAGTGCTACATGGAACAATATGGAGTATCAAAAGAGATAGCCGTAGCTGAACTGAAAAAGCAAGTGGGTGAAGCATGGAAAGAAATAATAGAAGATTACATAAAACGTGGCAAGTTTCCAAGTGTGATTCATGATTATGGACCCAATTTTGCTCGAGTCACAGACTTCTATTATAAGGAACGAGATGGTTTCACTTTTGCTAATACAGAAACTAAACATCTTATCGCCTTATTGCTTACAGAGCCTGTGCCAATTTGAACATATCTATAATCTACTTATTTAAACTTGTAATGCCTTTAATAAAAGTTGTAAGAATGCTTCGCAGTTTGAACTTAATATGTTTTCATGTACTTTGTTATGTGTGTTTTATTAA

Coding sequence (CDS)

ATGTCATTTCAAATAACTCCATCAGAATCCGAGGCAGCAAATAAACTTACTTTAAGCGACAATGATGTTCTTCGTCGCATTGAATACCATGAACCAAGTGCTTGGGGAGATTATTTCCTTTCCAATATTTCCAATCTTTCCATTCTTTTGGAAGAAGATTCAGTCCTGAAAAAACTTGAAGAACTTAAAGAAGAAGCGAGAAGCATGTTTGTAGCTGCTGAAAAGCATTCAGAAAAATTGAGTTTGATTGATTCACTCCAATGTTTAGGTTTGTCATACCACTTTGAAGATGAAATTAACAAAATACTTGATCAAATTCAAAATTCTGCCCATGTTGATGAAGAAGATGTGGAGGATCTTTACATTGTGGCTCTTAAATTCCGATTGCTTAGGCAACGAGGTTTCTTTGTTTCTTGTGAGATGTTCAAAAAGTTTACAAGTGAAAGTGGAGATTTCAAAGAGTCAATTACAAAAGATGAAAGAGGTTTATTAAGCTTGTATGAGGCATCTCACTTGAGAATGAAGGGAGAAAATATAATGGATGAAGCATTAGAATTCACATCAACTCAACTTGAAGCAATGGCAATGGATTCCACTTCTCCATTCTCAGAAGAAGCTAAATATGCATTAAAGTGGCCAATCTATAAAACTTTGCCAAGGTTCATGACTAGGAACTATATCTCTCTCTATCGCAACAATCCATTGAAGAATAGTAATATTTTGCTCACTTTTGCAAAGTTAGACTACAATTCTCTTCAAAAACTCTACCAAAGAGAACTTGGTGAACTTTCAAGGTGGTGGAATGATTTGAAGTTAAAAGAGAAATTACCTTTTGCAAGAGATAGAGTGGTGGAGAGCTATATTTGGGCATTGGGAATATACTATGAACCAAAGTACTCTAGTGCTAGAACAATAGTTGCCAAAATTATTGCTATCATAACAGTTATTGATGATATGTATGATTGTTATGGCACACTTGAAGAACTTCAACTATTCACCAAAGCAATTGAAAGATGGGATTTGAATTGCATTGAGGAGCTCCCAAACTACATGAAAGTTTTGTATGAAGCTATACTTGAATTTTATGAAGGGAGTATAAAAGATATGTGCATGGACAACAATATTCCATATGCATTTGATTATGCAAAAGAAGGGATAATAAGGCAATGTAAACTTTATATAGTGGAAGCTAAATGGTTCAATGAAGACTATGTGCCAACAATAGAAGAGTACATGAAAATTAGTGCCACTTCCATTGGCGTGTATGCTGTGGCTTCAATTGCCTTCCTATCTTTAGGAAATATTGTTTCAGAAGAGGTTTTCCAATGGGTTCAAGGTAACCCTATGCTTCATCAAGCAAGTGAAGCTGCTAGTAGACTTGTGAATGACATAGTTTCCCACAAGTTTGGAGAAGAGAGGTGCCATGTTACTTGTTCGATTGAGTGCTACATGGAACAATATGGAGTATCAAAAGAGATAGCCGTAGCTGAACTGAAAAAGCAAGTGGGTGAAGCATGGAAAGAAATAATAGAAGATTACATAAAACGTGGCAAGTTTCCAAGTGTGATTCATGATTATGGACCCAATTTTGCTCGAGTCACAGACTTCTATTATAAGGAACGAGATGGTTTCACTTTTGCTAATACAGAAACTAAACATCTTATCGCCTTATTGCTTACAGAGCCTGTGCCAATTTGA

Protein sequence

MSFQITPSESEAANKLTLSDNDVLRRIEYHEPSAWGDYFLSNISNLSILLEEDSVLKKLEELKEEARSMFVAAEKHSEKLSLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDLYIVALKFRLLRQRGFFVSCEMFKKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIMDEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKNSNILLTFAKLDYNSLQKLYQRELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKYSSARTIVAKIIAIITVIDDMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAILEFYEGSIKDMCMDNNIPYAFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSIGVYAVASIAFLSLGNIVSEEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCSIECYMEQYGVSKEIAVAELKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYKERDGFTFANTETKHLIALLLTEPVPI*
Homology
BLAST of CSPI02G27020 vs. ExPASy Swiss-Prot
Match: Q6Q3H3 ((-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 4.2e-133
Identity = 243/545 (44.59%), Postives = 357/545 (65.50%), Query Frame = 0

Query: 22  DVLRRIEYHEPSAWGDYFLSNISNLSILLEEDSVLKKLEELKEEARSMFVAAEKHS-EKL 81
           +V RR     PS WGD+FLS  S  +    +D + + +++LKEE R M +AA+  S +KL
Sbjct: 19  EVGRRCANFHPSIWGDHFLSYASEFT--NTDDHLKQHVQQLKEEVRKMLMAADDDSAQKL 78

Query: 82  SLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDLYIVALKFRLLRQRGFFVSCE 141
            LID++Q LG++YHFE EI+++L  + + + V  E  ED+Y  +L+FRLLRQ+G+ VSC+
Sbjct: 79  LLIDAIQRLGVAYHFESEIDEVLKHMFDGSVVSAE--EDVYTASLRFRLLRQQGYHVSCD 138

Query: 142 MFKKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIMDEALEFTSTQLEAMAMDSTS 201
           +F  F    G+FKES++ D RG+LSLYEA+H R+ GE+I+DEAL FT+T L++    S++
Sbjct: 139 LFNNFKDNEGNFKESLSSDVRGMLSLYEATHFRVHGEDILDEALAFTTTHLQSATKHSSN 198

Query: 202 PFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKNSNILLTFAKLDYNSLQKLYQREL 261
           P +E+  +ALK PI K LPR   R+Y S+Y+ +   N   LL  AKLD+N LQKL+Q+EL
Sbjct: 199 PLAEQVVHALKQPIRKGLPRLEARHYFSVYQADDSHN-KALLKLAKLDFNLLQKLHQKEL 258

Query: 262 GELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKYSSARTIVAKIIAIITVIDDMY 321
            ++S WW DL    KLPFARDRVVE Y W LG+Y+EP++  AR I+ K+IA+ ++IDD+Y
Sbjct: 259 SDISAWWKDLDFAHKLPFARDRVVECYFWILGVYFEPQFFFARRILTKVIAMTSIIDDIY 318

Query: 322 DCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAILEFYEGSIKDMCMDNNIPYAFD 381
           D YGTLEEL+LFT+A+ERWD++ I++LP YM+V Y+A+L  Y    ++M  +    Y   
Sbjct: 319 DVYGTLEELELFTEAVERWDISAIDQLPEYMRVCYQALLYVYSEIEEEMAKEGR-SYRLY 378

Query: 382 YAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSIGVYAVASIAFLSLGNIVSEEV 441
           YAKE +  Q + Y  EAKW     +PT+EEYM ++  +     +A+ +F+ +G+ V++E 
Sbjct: 379 YAKEAMKNQVRAYYEEAKWLQVQQIPTMEEYMPVALVTSAYSMLATTSFVGMGDAVTKES 438

Query: 442 FQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCSIECYMEQYGVSKEIAVAELKK 501
           F W+   P + +AS    RL++D+V HKF ++R HV  ++ECYM+Q+G S++    E  +
Sbjct: 439 FDWIFSKPKIVRASAIVCRLMDDMVFHKFEQKRGHVASAVECYMKQHGASEQETPNEFPQ 498

Query: 502 QVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYKERDGFTFANTETKHLIALLLT 561
            V EAWK+I E+ +     P  I     N ARV D  YK  DG+T      K  +  +L 
Sbjct: 499 PVREAWKDINEECLIPTAVPMPILMRVLNLARVIDVIYKNEDGYTHFGAVLKDFVTSMLI 557

Query: 562 EPVPI 566
           +PVPI
Sbjct: 559 DPVPI 557

BLAST of CSPI02G27020 vs. ExPASy Swiss-Prot
Match: B9SCB6 (Probable terpene synthase 2 OS=Ricinus communis OX=3988 GN=TPS2 PE=3 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 3.0e-131
Identity = 236/538 (43.87%), Postives = 352/538 (65.43%), Query Frame = 0

Query: 25  RRIEYHEPSAWGDYFLSNISNLSILLEEDSVLKKLEELKEEARSMFVAAEKHSEKLSLID 84
           R +    P+ WGD FLS  +  + +  ED + +++E LKEE + M  +A++ S+ L+LID
Sbjct: 17  RHLASFHPNIWGDRFLSCAAESTDI--EDDMEQQVERLKEEVKKMIASADEPSQILNLID 76

Query: 85  SLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDLYIVALKFRLLRQRGFFVSCEMFKK 144
            LQ LG+SYHFE EI + L Q+ N  + D +  +DL+ VAL+FRLLR++G  VSC++F K
Sbjct: 77  LLQRLGVSYHFEKEIEEALQQVLN-MNSDSDKDDDLHSVALRFRLLREQGLNVSCDVFNK 136

Query: 145 FTSESGDFKESITKDERGLLSLYEASHLRMKGENIMDEALEFTSTQLEAMAMDSTSPFSE 204
           F   +G F +++  D +G+LSLYEA+H R+ GE I+D+AL FT+T LE++  + + P + 
Sbjct: 137 FRDRNGHFIQTLKTDLQGMLSLYEAAHFRVHGEGILDDALAFTTTYLESIVPNLSPPLAA 196

Query: 205 EAKYALKWPIYKTLPRFMTRNYISLYRNNPLKNSNILLTFAKLDYNSLQKLYQRELGELS 264
           +    L+ P+ K+L R   R++IS+Y+ +   N  +LLTFAKLD+N LQKL+Q+EL  +S
Sbjct: 197 QISRTLRQPLRKSLARVEARHFISIYQEDTSHN-EVLLTFAKLDFNLLQKLHQKELKYIS 256

Query: 265 RWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKYSSARTIVAKIIAIITVIDDMYDCYG 324
            WW DL    KLPF RDRVVE Y W LG+Y+EP+Y  AR  V K+I +++VIDD+YD YG
Sbjct: 257 LWWKDLDFVNKLPFTRDRVVEGYFWILGVYFEPQYHRARKFVTKVINVVSVIDDIYDAYG 316

Query: 325 TLEELQLFTKAIERWDLNCIEELPNYMKVLYEAILEFYEGSIKDMCMDNNIPYAFDYAKE 384
           TLEEL +FT AI RWD++CI++LP YMKV Y+A+L  YE  I+    +    Y   YAKE
Sbjct: 317 TLEELVVFTDAINRWDIDCIDQLPEYMKVCYKALLNVYE-EIERALSEQGRSYRLHYAKE 376

Query: 385 GIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSIGVYAVASIAFLSLGNIVSEEVFQWV 444
            + +  + Y+VEA W N++YVPT++EYM I+  S     +   +F+ +G+I ++EVF W 
Sbjct: 377 AMKKLVQAYLVEANWMNKNYVPTMDEYMSIALVSCAYPLLTVTSFVGMGDIATKEVFDWA 436

Query: 445 QGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCSIECYMEQYGVSKEIAVAELKKQVGE 504
             +P + + +    RL++DIVSH+F ++R H+  S+ECYM+Q GVS+E    E  KQ+ +
Sbjct: 437 SNDPKIVRVASIICRLMDDIVSHEFEQKRGHIASSVECYMKQNGVSEEATRDEFNKQIVD 496

Query: 505 AWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYKERDGFTFANTETKHLIALLLTEP 563
           AWK+I E++++    P        N AR+ D+ YK+ D +T      K  +A LL +P
Sbjct: 497 AWKDINEEHLQPNYVPMPFRTRVVNSARIMDYLYKDDDEYTHVGELMKGSVAALLIDP 549

BLAST of CSPI02G27020 vs. ExPASy Swiss-Prot
Match: B2KSJ5 ((+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 7.5e-130
Identity = 250/554 (45.13%), Postives = 359/554 (64.80%), Query Frame = 0

Query: 22  DVLRRIEYHEPSAWGDYFLSNISNLSILLEEDSVLKKLEELKEEARSMFVA-AEKHSEKL 81
           DV R +    P+ W ++FLS   + ++ ++E  + ++ E+LKEE R M +A  E    KL
Sbjct: 20  DVKRSLANFHPNIWKEHFLSFTFDDALKIDE-GMKERTEKLKEEIRMMMIAYVENQLIKL 79

Query: 82  SLIDSLQCLGLSYHFEDEINKILDQI----QNSAHVDEEDV--EDLYIVALKFRLLRQRG 141
           +L+DS+Q LG+SYHFEDE+++ L+ I     NS  +  ++   EDL+I AL FRLLRQ+G
Sbjct: 80  NLVDSIQRLGVSYHFEDEVDEFLEHIYVSYNNSLLLSNKNSNGEDLHITALLFRLLRQQG 139

Query: 142 FFVSCEMFKKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIMDEALEFTSTQLEAM 201
           + +SC++F KF  ++G FKES+ +DERGLLSLYEASH+   GE +++EALEFT+T L+  
Sbjct: 140 YRISCDIFLKFMDDNGKFKESLVEDERGLLSLYEASHMMGHGEALLEEALEFTTTHLQTY 199

Query: 202 A---MDSTSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKNSNILLTFAKLDYNS 261
                +    F+ E   ALK PI K++PR   R Y+ +Y+ +P  N   LL F+KLD+N 
Sbjct: 200 IHRYSNINPSFASEVSNALKLPIRKSVPRIKAREYLEIYQQHPSHNET-LLEFSKLDFNI 259

Query: 262 LQKLYQRELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKYSSARTIVAKIIA 321
           LQKL+Q+EL E+ RWW DL +  K PFARDR+VE Y W LG Y+EP+YS  R ++ K+IA
Sbjct: 260 LQKLHQKELSEICRWWKDLDVPTKFPFARDRIVECYFWTLGAYFEPQYSVGRKMLTKVIA 319

Query: 322 IITVIDDMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAILEFYEGSIKDMCM 381
           I +++DD+YD YGT EELQ+ T AI+RWD + +  LP YMK  Y A+LE YE   K++  
Sbjct: 320 IASILDDIYDAYGTFEELQVLTPAIQRWDRSMVHTLPLYMKPFYVAMLELYEEIGKEIDK 379

Query: 382 DNNIPYAFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSIGVYAVASIAFLS 441
           D N  +    A  GI R  + Y  EAKW N++Y P+ +EYM+++  + G   + SI+FL 
Sbjct: 380 DQNSLH-LQVAIGGIKRLSESYFEEAKWLNKEYKPSFKEYMELALKTTGYTMLISISFLG 439

Query: 442 LG-NIVSEEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCSIECYMEQYGVS 501
           LG +IV+ EV QW+   P + +AS    RL++DI SHKF +ER HV  ++ECYM+QY  S
Sbjct: 440 LGDHIVTNEVLQWLSNGPQIIKASTIICRLMDDIASHKFEQEREHVASAVECYMKQYDCS 499

Query: 502 KEIAVAELKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYKERDGFTFANTE 561
           +E A  EL K+V +AWK+  E + +    P  +     NF+RV +  Y + DG+T A + 
Sbjct: 500 EEEACIELHKEVVDAWKDTNEAFYRPFNVPVPVLMRVLNFSRVINLLYLDEDGYTNAKSG 559

Query: 562 TKHLIALLLTEPVP 565
           TK LI  LL +P+P
Sbjct: 560 TKFLIKSLLVDPLP 570

BLAST of CSPI02G27020 vs. ExPASy Swiss-Prot
Match: P93665 ((+)-delta-cadinene synthase OS=Gossypium hirsutum OX=3635 GN=CDN1 PE=1 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 2.0e-127
Identity = 236/548 (43.07%), Postives = 346/548 (63.14%), Query Frame = 0

Query: 19  SDNDVLRRIEYHEPSAWGDYFLSNISNLSILLEEDSVLKKLEELKEEARSMFVAAEKHSE 78
           S+ D +R     +PS WGD+FL N  + +I  E     K+ ++LKEE R M VA   +S 
Sbjct: 16  SNKDEMRPKADFQPSIWGDFFL-NCPDKNIDAETQ---KRHQQLKEEVRKMIVAPMANST 75

Query: 79  -KLSLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDLYIVALKFRLLRQRGFFV 138
            KL+ IDS+Q LG+SYHF  EI    D+++N  H + +   DLY  +L+FRLLR+ GF V
Sbjct: 76  LKLAFIDSVQGLGVSYHFTKEIE---DELENIYHNNNDAENDLYTTSLRFRLLREHGFHV 135

Query: 139 SCEMFKKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIMDEALEFTSTQLEAMAMD 198
           SC++F KF  E G+FK S+T D RGLL LY+AS+LR+ GE+I+DEA+ FTS  L      
Sbjct: 136 SCDVFNKFKDEQGNFKSSVTSDVRGLLELYQASYLRVHGEDILDEAISFTSNHLSLAVAS 195

Query: 199 STSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKNSNILLTFAKLDYNSLQKLYQ 258
              P SEE  +ALK  I + LPR   R+Y+S+Y++    N  +LL FAK+D+N +Q L++
Sbjct: 196 LDHPLSEEVSHALKQSIRRGLPRVEARHYLSVYQDIESHN-KVLLEFAKIDFNMVQLLHR 255

Query: 259 RELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKYSSARTIVAKIIAIITVID 318
           +EL E+SRWW DL  + KLP+ARDRVVE Y W  G+Y+EP+YS  R ++ K+IA+ +++D
Sbjct: 256 KELSEISRWWKDLDFQRKLPYARDRVVEGYFWISGVYFEPQYSLGRKMLTKVIAMASIVD 315

Query: 319 DMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAILEFYEGSIKDMCMDNNIPY 378
           D YD Y T EEL  +T AIERWD+ CI+ELP YMK  Y+A+L+ YE  ++ +  ++   Y
Sbjct: 316 DTYDSYATYEELIPYTNAIERWDIKCIDELPEYMKPSYKALLDVYE-EMEQLVAEHGRQY 375

Query: 379 AFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSIGVYAVASIAFLSLGNIVS 438
             +YAK  +IR  + Y+VEA+W  ++Y P+ EE+   +  + G   +A  +F+ +G+IV+
Sbjct: 376 RVEYAKNAMIRLAQSYLVEARWTLQNYKPSFEEFKANALPTCGYAMLAITSFVGMGDIVT 435

Query: 439 EEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCSIECYMEQYGVSKEIAVAE 498
            E F+W   +P + QAS    R ++D+  HKF   R     +IECYME+YGV+ + A   
Sbjct: 436 PETFKWAANDPKIIQASTIICRFMDDVTEHKFKHRREDDCSAIECYMEEYGVTAQEAYDV 495

Query: 499 LKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYKERDGFTFANTETKHLIAL 558
             K V  AWK++ + ++K  + P+ + +   N ARV D  Y+E DG+T+     K  I  
Sbjct: 496 FNKHVESAWKDVNQGFLKPTEMPTEVLNRSLNLARVMDVLYREGDGYTYVGKAAKGGITS 554

Query: 559 LLTEPVPI 566
           LL EP+ +
Sbjct: 556 LLIEPIAL 554

BLAST of CSPI02G27020 vs. ExPASy Swiss-Prot
Match: Q39760 ((+)-delta-cadinene synthase isozyme XC14 OS=Gossypium arboreum OX=29729 PE=2 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 2.0e-127
Identity = 237/548 (43.25%), Postives = 348/548 (63.50%), Query Frame = 0

Query: 19  SDNDVLRRIEYHEPSAWGDYFLSNISNLSILLEEDSVLKKLEELKEEARSMFVAAEKHS- 78
           S+ D +R     +PS WGD FL N  + +I  E +   K+ ++LKEE R M VA   +S 
Sbjct: 16  SNKDEMRPKADFQPSIWGDLFL-NCPDKNIDAETE---KRHQQLKEEVRKMIVAPMANST 75

Query: 79  EKLSLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDLYIVALKFRLLRQRGFFV 138
           +KL+ IDS+Q LG+SYHF  EI    D+++N  H + +   DLY  +L+FRLLR+ GF V
Sbjct: 76  QKLAFIDSVQRLGVSYHFTKEIE---DELENIYHNNNDAENDLYTTSLRFRLLREHGFNV 135

Query: 139 SCEMFKKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIMDEALEFTSTQLEAMAMD 198
           SC++F KF  E G+FK S+T D RGLL LY+AS+LR+ GE+I+DEA+ FT+  L      
Sbjct: 136 SCDVFNKFKDEQGNFKSSVTSDVRGLLELYQASYLRVHGEDILDEAISFTTNHLSLAVAS 195

Query: 199 STSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKNSNILLTFAKLDYNSLQKLYQ 258
              P SEE  +ALK  I + LPR   R+Y+S+Y++    N  +LL FAK+D+N +Q L++
Sbjct: 196 LDYPLSEEVSHALKQSIRRGLPRVEARHYLSVYQDIESHN-KVLLEFAKIDFNMVQLLHR 255

Query: 259 RELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKYSSARTIVAKIIAIITVID 318
           +EL E+SRWW DL  + KLP+ARDRVVE Y W  G+Y+EP+YS  R ++ K+IA+ +++D
Sbjct: 256 KELSEISRWWKDLDFQRKLPYARDRVVEGYFWISGVYFEPQYSLGRKMLTKVIAMASIVD 315

Query: 319 DMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAILEFYEGSIKDMCMDNNIPY 378
           D YD Y T EEL  +TKAIERWD+ CI+ELP YMK  Y+A+L+ YE  ++ +   +   Y
Sbjct: 316 DTYDSYATYEELIPYTKAIERWDIKCIDELPEYMKPSYKALLDVYE-EMEQLVAKHGRQY 375

Query: 379 AFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSIGVYAVASIAFLSLGNIVS 438
             +YAK  +IR  + Y+VEA+W  ++Y P+ EE+   +  + G   +A  +F+ +G+IV+
Sbjct: 376 RVEYAKNAMIRLAQSYLVEARWTLQNYKPSFEEFKANALPTCGYAMLAITSFVGMGDIVT 435

Query: 439 EEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCSIECYMEQYGVSKEIAVAE 498
            E F+W   +P + QAS    R ++D+  HKF   R     +IECYME+YGV+ + A   
Sbjct: 436 PETFKWAANDPKIIQASTIICRFMDDVAEHKFKHRREDDCSAIECYMEEYGVTAQEAYDV 495

Query: 499 LKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYKERDGFTFANTETKHLIAL 558
             K V  AWK++ ++++K  + P+ + +   N ARV D  Y+E DG+T+     K  I  
Sbjct: 496 FNKHVESAWKDVNKEFLKPTEMPTEVLNRSLNLARVMDVLYREGDGYTYVGKAAKGGITS 554

Query: 559 LLTEPVPI 566
           LL EPV +
Sbjct: 556 LLIEPVAL 554

BLAST of CSPI02G27020 vs. ExPASy TrEMBL
Match: A0A0A0LN25 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G427840 PE=4 SV=1)

HSP 1 Score: 1120.9 bits (2898), Expect = 0.0e+00
Identity = 561/565 (99.29%), Postives = 562/565 (99.47%), Query Frame = 0

Query: 1   MSFQITPSESEAANKLTLSDNDVLRRIEYHEPSAWGDYFLSNISNLSILLEEDSVLKKLE 60
           MSFQITPSESEAANKLTLSDNDVLRRIEYHEPSAWGDYFLSNISNLS LLEEDSVLKKLE
Sbjct: 1   MSFQITPSESEAANKLTLSDNDVLRRIEYHEPSAWGDYFLSNISNLSNLLEEDSVLKKLE 60

Query: 61  ELKEEARSMFVAAEKHSEKLSLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDL 120
           ELKEEARSMFVAAEKHSEKLSLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDL
Sbjct: 61  ELKEEARSMFVAAEKHSEKLSLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDL 120

Query: 121 YIVALKFRLLRQRGFFVSCEMFKKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIM 180
           YIVALKFRLLRQRGFFVSCE+ KKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIM
Sbjct: 121 YIVALKFRLLRQRGFFVSCEILKKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIM 180

Query: 181 DEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKNSNI 240
           DEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLK SNI
Sbjct: 181 DEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKKSNI 240

Query: 241 LLTFAKLDYNSLQKLYQRELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKYS 300
           LLTFAKLDYNSLQKLYQRELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKYS
Sbjct: 241 LLTFAKLDYNSLQKLYQRELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKYS 300

Query: 301 SARTIVAKIIAIITVIDDMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAILE 360
           SARTIVAKIIAIITVIDDMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAILE
Sbjct: 301 SARTIVAKIIAIITVIDDMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAILE 360

Query: 361 FYEGSIKDMCMDNNIPYAFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSIG 420
           FYEGSIKDMCMDNNIPYAFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSIG
Sbjct: 361 FYEGSIKDMCMDNNIPYAFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSIG 420

Query: 421 VYAVASIAFLSLGNIVSEEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCSI 480
           VYAVASIAFLSLGNIVSEEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCSI
Sbjct: 421 VYAVASIAFLSLGNIVSEEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCSI 480

Query: 481 ECYMEQYGVSKEIAVAELKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYKE 540
           ECYMEQYGVSKEIAVAELKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYKE
Sbjct: 481 ECYMEQYGVSKEIAVAELKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYKE 540

Query: 541 RDGFTFANTETKHLIALLLTEPVPI 566
           RDGFTFANTETKHLIALLLTEPVPI
Sbjct: 541 RDGFTFANTETKHLIALLLTEPVPI 565

BLAST of CSPI02G27020 vs. ExPASy TrEMBL
Match: A0A1S3CHV0 (probable terpene synthase 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501122 PE=4 SV=1)

HSP 1 Score: 1021.1 bits (2639), Expect = 1.7e-294
Identity = 503/566 (88.87%), Postives = 539/566 (95.23%), Query Frame = 0

Query: 1   MSFQITPSESEAANKLTLSDNDVLRRIEYHEPSAWGDYFLSNISNLSILLEEDSVLKKLE 60
           MSFQ   SESEA NKLTLSDN VLRR EYH+PSAWGDYFLSN+SN   +LEEDSVLKKLE
Sbjct: 1   MSFQTIASESEAPNKLTLSDNGVLRRTEYHQPSAWGDYFLSNLSN---ILEEDSVLKKLE 60

Query: 61  ELKEEARSMFVAAEKHSEKLSLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDL 120
           ELKE ARSMFVAAEKHS+KL LIDS+QCLGLSYHFEDEIN+ILDQIQNSAHVDEED EDL
Sbjct: 61  ELKEAARSMFVAAEKHSDKLCLIDSIQCLGLSYHFEDEINEILDQIQNSAHVDEEDEEDL 120

Query: 121 YIVALKFRLLRQRGFFVSCEMFKKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIM 180
           YI+ALKFRLLRQ+GF VSCE+F KFT+ESGDFKESITKDE+GLLSLYEASHLRMKGE+IM
Sbjct: 121 YIIALKFRLLRQQGFPVSCEIFNKFTTESGDFKESITKDEKGLLSLYEASHLRMKGESIM 180

Query: 181 DEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKN-SN 240
           DEALEFT+TQLEAMAMDSTSPFS+EAKYALKWPIYKTL RFMTR+YISLY NNP KN +N
Sbjct: 181 DEALEFTTTQLEAMAMDSTSPFSQEAKYALKWPIYKTLSRFMTRHYISLYHNNPTKNDNN 240

Query: 241 ILLTFAKLDYNSLQKLYQRELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKY 300
           ILLTFAKLDYNSLQKLYQRELGELSRWWND KLKEKLPFARDRVVESYIWALGIYYEPKY
Sbjct: 241 ILLTFAKLDYNSLQKLYQRELGELSRWWNDSKLKEKLPFARDRVVESYIWALGIYYEPKY 300

Query: 301 SSARTIVAKIIAIITVIDDMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAIL 360
           SSART++AKIIA+IT+IDDMYDCYGTLEELQLFT  I RWD+NCIEELPNYMKVLYEAIL
Sbjct: 301 SSARTLLAKIIALITLIDDMYDCYGTLEELQLFTNTIGRWDMNCIEELPNYMKVLYEAIL 360

Query: 361 EFYEGSIKDMCMDNNIPYAFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSI 420
           + YEG+I+DMCMDNNIPYAFDYAKEGIIRQCKLY+VEAKWFNEDYVPTIEEYMK+SATSI
Sbjct: 361 KIYEGTIQDMCMDNNIPYAFDYAKEGIIRQCKLYMVEAKWFNEDYVPTIEEYMKVSATSI 420

Query: 421 GVYAVASIAFLSLGNIVSEEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCS 480
           G+YAVASIAFLS+GNIVSEE+FQWVQGNPMLH+ASE ASRLVNDIVSHKFG+ERCHVTCS
Sbjct: 421 GLYAVASIAFLSIGNIVSEEIFQWVQGNPMLHKASEVASRLVNDIVSHKFGQERCHVTCS 480

Query: 481 IECYMEQYGVSKEIAVAELKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYK 540
           IECYMEQYGVSKE+A+AELKKQV EA KEIIEDYIKRGKFP VIHDYGPNFARVTDFYYK
Sbjct: 481 IECYMEQYGVSKEVAIAELKKQVDEARKEIIEDYIKRGKFPRVIHDYGPNFARVTDFYYK 540

Query: 541 ERDGFTFANTETKHLIALLLTEPVPI 566
           ERDGFTFANTETKHLIAL+LT+P+PI
Sbjct: 541 ERDGFTFANTETKHLIALILTDPMPI 563

BLAST of CSPI02G27020 vs. ExPASy TrEMBL
Match: A0A5D3BQ16 (Putative terpene synthase 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G00170 PE=4 SV=1)

HSP 1 Score: 1020.4 bits (2637), Expect = 2.9e-294
Identity = 502/568 (88.38%), Postives = 540/568 (95.07%), Query Frame = 0

Query: 1   MSFQITPSESEAANKLTLSDNDVLRRIEYHEPSAWGDYFLSNISNLSI--LLEEDSVLKK 60
           MSFQ   SESEA NKLTLSDN VLRR EYH+PSAWGDYFLSN+SN+ +  + EEDSVLKK
Sbjct: 1   MSFQTIASESEAPNKLTLSDNGVLRRTEYHQPSAWGDYFLSNLSNILVMNIQEEDSVLKK 60

Query: 61  LEELKEEARSMFVAAEKHSEKLSLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVE 120
           LEELKE ARSMFVAAEKHS+KL LIDS+QCLGLSYHFEDEIN+ILDQIQNSAHVDEED E
Sbjct: 61  LEELKEAARSMFVAAEKHSDKLCLIDSIQCLGLSYHFEDEINEILDQIQNSAHVDEEDEE 120

Query: 121 DLYIVALKFRLLRQRGFFVSCEMFKKFTSESGDFKESITKDERGLLSLYEASHLRMKGEN 180
           DLYI+ALKFRLLRQ+GF VSCE+F KFT+ESGDFKESITKDE+GLLSLYEASHLRMKGE+
Sbjct: 121 DLYIIALKFRLLRQQGFPVSCEIFNKFTTESGDFKESITKDEKGLLSLYEASHLRMKGES 180

Query: 181 IMDEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKN- 240
           IMDEALEFT+TQLEAMAMDSTSPFS+EAKYALKWPIYKTL RFMTR+YISLY NNP KN 
Sbjct: 181 IMDEALEFTTTQLEAMAMDSTSPFSQEAKYALKWPIYKTLSRFMTRHYISLYHNNPTKND 240

Query: 241 SNILLTFAKLDYNSLQKLYQRELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEP 300
           +NILLTFAKLDYNSLQKLYQRELGELSRWWND KLKEKLPFARDRVVESYIWALGIYYEP
Sbjct: 241 NNILLTFAKLDYNSLQKLYQRELGELSRWWNDSKLKEKLPFARDRVVESYIWALGIYYEP 300

Query: 301 KYSSARTIVAKIIAIITVIDDMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEA 360
           KYSSART++AKIIA+IT+IDDMYDCYGTLEELQLFT  I RWD+NCIEELPNYMKVLYEA
Sbjct: 301 KYSSARTLLAKIIALITLIDDMYDCYGTLEELQLFTNTIGRWDMNCIEELPNYMKVLYEA 360

Query: 361 ILEFYEGSIKDMCMDNNIPYAFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISAT 420
           IL+ YEG+I+DMCMDNNIPYAFDYAKEGIIRQCKLY+VEAKWFNEDYVPTIEEYMK+SAT
Sbjct: 361 ILKIYEGTIQDMCMDNNIPYAFDYAKEGIIRQCKLYMVEAKWFNEDYVPTIEEYMKVSAT 420

Query: 421 SIGVYAVASIAFLSLGNIVSEEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVT 480
           SIG+YAVASIAFLS+GNIVSEE+FQWVQGNPMLH+ASE ASRLVNDIVSHKFG+ERCHVT
Sbjct: 421 SIGLYAVASIAFLSIGNIVSEEIFQWVQGNPMLHKASEVASRLVNDIVSHKFGQERCHVT 480

Query: 481 CSIECYMEQYGVSKEIAVAELKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFY 540
           CSIECYMEQYGVSKE+A+AELKKQV EA KEIIEDYIKRGKFP VIHDYGPNFARVTDFY
Sbjct: 481 CSIECYMEQYGVSKEVAIAELKKQVDEARKEIIEDYIKRGKFPRVIHDYGPNFARVTDFY 540

Query: 541 YKERDGFTFANTETKHLIALLLTEPVPI 566
           YKERDGFTFANTETKHLIAL+LT+P+PI
Sbjct: 541 YKERDGFTFANTETKHLIALILTDPMPI 568

BLAST of CSPI02G27020 vs. ExPASy TrEMBL
Match: A0A5A7VDK3 (Putative terpene synthase 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold21G005040 PE=4 SV=1)

HSP 1 Score: 1017.3 bits (2629), Expect = 2.5e-293
Identity = 502/566 (88.69%), Postives = 538/566 (95.05%), Query Frame = 0

Query: 1   MSFQITPSESEAANKLTLSDNDVLRRIEYHEPSAWGDYFLSNISNLSILLEEDSVLKKLE 60
           MSFQ   SESEA NKLTLSDN VLRR EYH+PSAWGDYFLSN+SN   +LEEDSVLKKLE
Sbjct: 1   MSFQTIASESEAPNKLTLSDNGVLRRTEYHQPSAWGDYFLSNLSN---ILEEDSVLKKLE 60

Query: 61  ELKEEARSMFVAAEKHSEKLSLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDL 120
           ELKE ARSMFVAAEKHS+KL LIDS+QCLGLSYHFEDEIN+ILDQIQNSAHVDEED EDL
Sbjct: 61  ELKEAARSMFVAAEKHSDKLCLIDSIQCLGLSYHFEDEINEILDQIQNSAHVDEEDEEDL 120

Query: 121 YIVALKFRLLRQRGFFVSCEMFKKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIM 180
           YI+ALKFRLLRQ+GF VSCE+F KFT+ESGDFKESITKDE+GLLSLYEASHLRMKGE+IM
Sbjct: 121 YIIALKFRLLRQQGFPVSCEIFNKFTTESGDFKESITKDEKGLLSLYEASHLRMKGESIM 180

Query: 181 DEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKN-SN 240
           DEALEFT+TQLEAMAMDSTSPFS+EAKYALKWPIYKTL RFMTR+YISLY NNP KN +N
Sbjct: 181 DEALEFTTTQLEAMAMDSTSPFSQEAKYALKWPIYKTLSRFMTRHYISLYHNNPTKNDNN 240

Query: 241 ILLTFAKLDYNSLQKLYQRELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKY 300
           ILLTFAKLDYNSLQKLYQRELGELSRWWND KLKEKLPFARDRVVESYIWALGIYYEPKY
Sbjct: 241 ILLTFAKLDYNSLQKLYQRELGELSRWWNDSKLKEKLPFARDRVVESYIWALGIYYEPKY 300

Query: 301 SSARTIVAKIIAIITVIDDMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAIL 360
           SSART++AKIIA+IT+IDDMYDCYGTLEELQLFT  I RWD+NCIEELPNYMKVLYEAIL
Sbjct: 301 SSARTLLAKIIALITLIDDMYDCYGTLEELQLFTNTIGRWDMNCIEELPNYMKVLYEAIL 360

Query: 361 EFYEGSIKDMCMDNNIPYAFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSI 420
           + YEG+I+DMCMDNNIPYAFDYAKEGIIRQCKLY+VEAKWFNEDYVPTIEEYMK+SATSI
Sbjct: 361 KIYEGTIQDMCMDNNIPYAFDYAKEGIIRQCKLYMVEAKWFNEDYVPTIEEYMKVSATSI 420

Query: 421 GVYAVASIAFLSLGNIVSEEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCS 480
           G+YAVASIAFLS+GNIVSEE+FQWVQGNPMLH+ASE ASRLVNDIVSHKFG+ERCHVTCS
Sbjct: 421 GLYAVASIAFLSIGNIVSEEIFQWVQGNPMLHKASEVASRLVNDIVSHKFGQERCHVTCS 480

Query: 481 IECYMEQYGVSKEIAVAELKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYK 540
           IECYMEQYGVSKE+A+AELKKQV EA KEIIEDYIKRGKFP VIHD GPNFARVTDFYYK
Sbjct: 481 IECYMEQYGVSKEVAIAELKKQVDEARKEIIEDYIKRGKFPRVIHDDGPNFARVTDFYYK 540

Query: 541 ERDGFTFANTETKHLIALLLTEPVPI 566
           ERDGFTFANTETKHLIAL+LT+P+PI
Sbjct: 541 ERDGFTFANTETKHLIALILTDPMPI 563

BLAST of CSPI02G27020 vs. ExPASy TrEMBL
Match: A0A1S3CHV8 (probable terpene synthase 2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103501122 PE=4 SV=1)

HSP 1 Score: 1005.0 bits (2597), Expect = 1.3e-289
Identity = 498/566 (87.99%), Postives = 534/566 (94.35%), Query Frame = 0

Query: 1   MSFQITPSESEAANKLTLSDNDVLRRIEYHEPSAWGDYFLSNISNLSILLEEDSVLKKLE 60
           MSFQ   SESEA NKLTLSDN VLRR EYH+PSAWGDYFLSN+SN   +LEEDSVLKKLE
Sbjct: 1   MSFQTIASESEAPNKLTLSDNGVLRRTEYHQPSAWGDYFLSNLSN---ILEEDSVLKKLE 60

Query: 61  ELKEEARSMFVAAEKHSEKLSLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDL 120
           ELKE ARSMFVAAEKHS+KL LIDS+QCLGLSYHFEDEIN+ILDQIQNSAHVDEED EDL
Sbjct: 61  ELKEAARSMFVAAEKHSDKLCLIDSIQCLGLSYHFEDEINEILDQIQNSAHVDEEDEEDL 120

Query: 121 YIVALKFRLLRQRGFFVSCEMFKKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIM 180
           YI+ALKFRLLRQ+      E+F KFT+ESGDFKESITKDE+GLLSLYEASHLRMKGE+IM
Sbjct: 121 YIIALKFRLLRQQ------EIFNKFTTESGDFKESITKDEKGLLSLYEASHLRMKGESIM 180

Query: 181 DEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKN-SN 240
           DEALEFT+TQLEAMAMDSTSPFS+EAKYALKWPIYKTL RFMTR+YISLY NNP KN +N
Sbjct: 181 DEALEFTTTQLEAMAMDSTSPFSQEAKYALKWPIYKTLSRFMTRHYISLYHNNPTKNDNN 240

Query: 241 ILLTFAKLDYNSLQKLYQRELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKY 300
           ILLTFAKLDYNSLQKLYQRELGELSRWWND KLKEKLPFARDRVVESYIWALGIYYEPKY
Sbjct: 241 ILLTFAKLDYNSLQKLYQRELGELSRWWNDSKLKEKLPFARDRVVESYIWALGIYYEPKY 300

Query: 301 SSARTIVAKIIAIITVIDDMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAIL 360
           SSART++AKIIA+IT+IDDMYDCYGTLEELQLFT  I RWD+NCIEELPNYMKVLYEAIL
Sbjct: 301 SSARTLLAKIIALITLIDDMYDCYGTLEELQLFTNTIGRWDMNCIEELPNYMKVLYEAIL 360

Query: 361 EFYEGSIKDMCMDNNIPYAFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSI 420
           + YEG+I+DMCMDNNIPYAFDYAKEGIIRQCKLY+VEAKWFNEDYVPTIEEYMK+SATSI
Sbjct: 361 KIYEGTIQDMCMDNNIPYAFDYAKEGIIRQCKLYMVEAKWFNEDYVPTIEEYMKVSATSI 420

Query: 421 GVYAVASIAFLSLGNIVSEEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCS 480
           G+YAVASIAFLS+GNIVSEE+FQWVQGNPMLH+ASE ASRLVNDIVSHKFG+ERCHVTCS
Sbjct: 421 GLYAVASIAFLSIGNIVSEEIFQWVQGNPMLHKASEVASRLVNDIVSHKFGQERCHVTCS 480

Query: 481 IECYMEQYGVSKEIAVAELKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYK 540
           IECYMEQYGVSKE+A+AELKKQV EA KEIIEDYIKRGKFP VIHDYGPNFARVTDFYYK
Sbjct: 481 IECYMEQYGVSKEVAIAELKKQVDEARKEIIEDYIKRGKFPRVIHDYGPNFARVTDFYYK 540

Query: 541 ERDGFTFANTETKHLIALLLTEPVPI 566
           ERDGFTFANTETKHLIAL+LT+P+PI
Sbjct: 541 ERDGFTFANTETKHLIALILTDPMPI 557

BLAST of CSPI02G27020 vs. NCBI nr
Match: XP_004151916.2 ((-)-germacrene D synthase [Cucumis sativus] >KGN63310.1 hypothetical protein Csa_022469 [Cucumis sativus])

HSP 1 Score: 1120.9 bits (2898), Expect = 0.0e+00
Identity = 561/565 (99.29%), Postives = 562/565 (99.47%), Query Frame = 0

Query: 1   MSFQITPSESEAANKLTLSDNDVLRRIEYHEPSAWGDYFLSNISNLSILLEEDSVLKKLE 60
           MSFQITPSESEAANKLTLSDNDVLRRIEYHEPSAWGDYFLSNISNLS LLEEDSVLKKLE
Sbjct: 1   MSFQITPSESEAANKLTLSDNDVLRRIEYHEPSAWGDYFLSNISNLSNLLEEDSVLKKLE 60

Query: 61  ELKEEARSMFVAAEKHSEKLSLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDL 120
           ELKEEARSMFVAAEKHSEKLSLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDL
Sbjct: 61  ELKEEARSMFVAAEKHSEKLSLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDL 120

Query: 121 YIVALKFRLLRQRGFFVSCEMFKKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIM 180
           YIVALKFRLLRQRGFFVSCE+ KKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIM
Sbjct: 121 YIVALKFRLLRQRGFFVSCEILKKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIM 180

Query: 181 DEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKNSNI 240
           DEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLK SNI
Sbjct: 181 DEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKKSNI 240

Query: 241 LLTFAKLDYNSLQKLYQRELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKYS 300
           LLTFAKLDYNSLQKLYQRELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKYS
Sbjct: 241 LLTFAKLDYNSLQKLYQRELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKYS 300

Query: 301 SARTIVAKIIAIITVIDDMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAILE 360
           SARTIVAKIIAIITVIDDMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAILE
Sbjct: 301 SARTIVAKIIAIITVIDDMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAILE 360

Query: 361 FYEGSIKDMCMDNNIPYAFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSIG 420
           FYEGSIKDMCMDNNIPYAFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSIG
Sbjct: 361 FYEGSIKDMCMDNNIPYAFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSIG 420

Query: 421 VYAVASIAFLSLGNIVSEEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCSI 480
           VYAVASIAFLSLGNIVSEEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCSI
Sbjct: 421 VYAVASIAFLSLGNIVSEEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCSI 480

Query: 481 ECYMEQYGVSKEIAVAELKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYKE 540
           ECYMEQYGVSKEIAVAELKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYKE
Sbjct: 481 ECYMEQYGVSKEIAVAELKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYKE 540

Query: 541 RDGFTFANTETKHLIALLLTEPVPI 566
           RDGFTFANTETKHLIALLLTEPVPI
Sbjct: 541 RDGFTFANTETKHLIALLLTEPVPI 565

BLAST of CSPI02G27020 vs. NCBI nr
Match: XP_008462842.2 (PREDICTED: probable terpene synthase 2 isoform X1 [Cucumis melo])

HSP 1 Score: 1021.1 bits (2639), Expect = 3.5e-294
Identity = 503/566 (88.87%), Postives = 539/566 (95.23%), Query Frame = 0

Query: 1   MSFQITPSESEAANKLTLSDNDVLRRIEYHEPSAWGDYFLSNISNLSILLEEDSVLKKLE 60
           MSFQ   SESEA NKLTLSDN VLRR EYH+PSAWGDYFLSN+SN   +LEEDSVLKKLE
Sbjct: 1   MSFQTIASESEAPNKLTLSDNGVLRRTEYHQPSAWGDYFLSNLSN---ILEEDSVLKKLE 60

Query: 61  ELKEEARSMFVAAEKHSEKLSLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDL 120
           ELKE ARSMFVAAEKHS+KL LIDS+QCLGLSYHFEDEIN+ILDQIQNSAHVDEED EDL
Sbjct: 61  ELKEAARSMFVAAEKHSDKLCLIDSIQCLGLSYHFEDEINEILDQIQNSAHVDEEDEEDL 120

Query: 121 YIVALKFRLLRQRGFFVSCEMFKKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIM 180
           YI+ALKFRLLRQ+GF VSCE+F KFT+ESGDFKESITKDE+GLLSLYEASHLRMKGE+IM
Sbjct: 121 YIIALKFRLLRQQGFPVSCEIFNKFTTESGDFKESITKDEKGLLSLYEASHLRMKGESIM 180

Query: 181 DEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKN-SN 240
           DEALEFT+TQLEAMAMDSTSPFS+EAKYALKWPIYKTL RFMTR+YISLY NNP KN +N
Sbjct: 181 DEALEFTTTQLEAMAMDSTSPFSQEAKYALKWPIYKTLSRFMTRHYISLYHNNPTKNDNN 240

Query: 241 ILLTFAKLDYNSLQKLYQRELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKY 300
           ILLTFAKLDYNSLQKLYQRELGELSRWWND KLKEKLPFARDRVVESYIWALGIYYEPKY
Sbjct: 241 ILLTFAKLDYNSLQKLYQRELGELSRWWNDSKLKEKLPFARDRVVESYIWALGIYYEPKY 300

Query: 301 SSARTIVAKIIAIITVIDDMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAIL 360
           SSART++AKIIA+IT+IDDMYDCYGTLEELQLFT  I RWD+NCIEELPNYMKVLYEAIL
Sbjct: 301 SSARTLLAKIIALITLIDDMYDCYGTLEELQLFTNTIGRWDMNCIEELPNYMKVLYEAIL 360

Query: 361 EFYEGSIKDMCMDNNIPYAFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSI 420
           + YEG+I+DMCMDNNIPYAFDYAKEGIIRQCKLY+VEAKWFNEDYVPTIEEYMK+SATSI
Sbjct: 361 KIYEGTIQDMCMDNNIPYAFDYAKEGIIRQCKLYMVEAKWFNEDYVPTIEEYMKVSATSI 420

Query: 421 GVYAVASIAFLSLGNIVSEEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCS 480
           G+YAVASIAFLS+GNIVSEE+FQWVQGNPMLH+ASE ASRLVNDIVSHKFG+ERCHVTCS
Sbjct: 421 GLYAVASIAFLSIGNIVSEEIFQWVQGNPMLHKASEVASRLVNDIVSHKFGQERCHVTCS 480

Query: 481 IECYMEQYGVSKEIAVAELKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYK 540
           IECYMEQYGVSKE+A+AELKKQV EA KEIIEDYIKRGKFP VIHDYGPNFARVTDFYYK
Sbjct: 481 IECYMEQYGVSKEVAIAELKKQVDEARKEIIEDYIKRGKFPRVIHDYGPNFARVTDFYYK 540

Query: 541 ERDGFTFANTETKHLIALLLTEPVPI 566
           ERDGFTFANTETKHLIAL+LT+P+PI
Sbjct: 541 ERDGFTFANTETKHLIALILTDPMPI 563

BLAST of CSPI02G27020 vs. NCBI nr
Match: TYK00862.1 (putative terpene synthase 2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1020.4 bits (2637), Expect = 6.0e-294
Identity = 502/568 (88.38%), Postives = 540/568 (95.07%), Query Frame = 0

Query: 1   MSFQITPSESEAANKLTLSDNDVLRRIEYHEPSAWGDYFLSNISNLSI--LLEEDSVLKK 60
           MSFQ   SESEA NKLTLSDN VLRR EYH+PSAWGDYFLSN+SN+ +  + EEDSVLKK
Sbjct: 1   MSFQTIASESEAPNKLTLSDNGVLRRTEYHQPSAWGDYFLSNLSNILVMNIQEEDSVLKK 60

Query: 61  LEELKEEARSMFVAAEKHSEKLSLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVE 120
           LEELKE ARSMFVAAEKHS+KL LIDS+QCLGLSYHFEDEIN+ILDQIQNSAHVDEED E
Sbjct: 61  LEELKEAARSMFVAAEKHSDKLCLIDSIQCLGLSYHFEDEINEILDQIQNSAHVDEEDEE 120

Query: 121 DLYIVALKFRLLRQRGFFVSCEMFKKFTSESGDFKESITKDERGLLSLYEASHLRMKGEN 180
           DLYI+ALKFRLLRQ+GF VSCE+F KFT+ESGDFKESITKDE+GLLSLYEASHLRMKGE+
Sbjct: 121 DLYIIALKFRLLRQQGFPVSCEIFNKFTTESGDFKESITKDEKGLLSLYEASHLRMKGES 180

Query: 181 IMDEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKN- 240
           IMDEALEFT+TQLEAMAMDSTSPFS+EAKYALKWPIYKTL RFMTR+YISLY NNP KN 
Sbjct: 181 IMDEALEFTTTQLEAMAMDSTSPFSQEAKYALKWPIYKTLSRFMTRHYISLYHNNPTKND 240

Query: 241 SNILLTFAKLDYNSLQKLYQRELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEP 300
           +NILLTFAKLDYNSLQKLYQRELGELSRWWND KLKEKLPFARDRVVESYIWALGIYYEP
Sbjct: 241 NNILLTFAKLDYNSLQKLYQRELGELSRWWNDSKLKEKLPFARDRVVESYIWALGIYYEP 300

Query: 301 KYSSARTIVAKIIAIITVIDDMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEA 360
           KYSSART++AKIIA+IT+IDDMYDCYGTLEELQLFT  I RWD+NCIEELPNYMKVLYEA
Sbjct: 301 KYSSARTLLAKIIALITLIDDMYDCYGTLEELQLFTNTIGRWDMNCIEELPNYMKVLYEA 360

Query: 361 ILEFYEGSIKDMCMDNNIPYAFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISAT 420
           IL+ YEG+I+DMCMDNNIPYAFDYAKEGIIRQCKLY+VEAKWFNEDYVPTIEEYMK+SAT
Sbjct: 361 ILKIYEGTIQDMCMDNNIPYAFDYAKEGIIRQCKLYMVEAKWFNEDYVPTIEEYMKVSAT 420

Query: 421 SIGVYAVASIAFLSLGNIVSEEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVT 480
           SIG+YAVASIAFLS+GNIVSEE+FQWVQGNPMLH+ASE ASRLVNDIVSHKFG+ERCHVT
Sbjct: 421 SIGLYAVASIAFLSIGNIVSEEIFQWVQGNPMLHKASEVASRLVNDIVSHKFGQERCHVT 480

Query: 481 CSIECYMEQYGVSKEIAVAELKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFY 540
           CSIECYMEQYGVSKE+A+AELKKQV EA KEIIEDYIKRGKFP VIHDYGPNFARVTDFY
Sbjct: 481 CSIECYMEQYGVSKEVAIAELKKQVDEARKEIIEDYIKRGKFPRVIHDYGPNFARVTDFY 540

Query: 541 YKERDGFTFANTETKHLIALLLTEPVPI 566
           YKERDGFTFANTETKHLIAL+LT+P+PI
Sbjct: 541 YKERDGFTFANTETKHLIALILTDPMPI 568

BLAST of CSPI02G27020 vs. NCBI nr
Match: KAA0066422.1 (putative terpene synthase 2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1017.3 bits (2629), Expect = 5.1e-293
Identity = 502/566 (88.69%), Postives = 538/566 (95.05%), Query Frame = 0

Query: 1   MSFQITPSESEAANKLTLSDNDVLRRIEYHEPSAWGDYFLSNISNLSILLEEDSVLKKLE 60
           MSFQ   SESEA NKLTLSDN VLRR EYH+PSAWGDYFLSN+SN   +LEEDSVLKKLE
Sbjct: 1   MSFQTIASESEAPNKLTLSDNGVLRRTEYHQPSAWGDYFLSNLSN---ILEEDSVLKKLE 60

Query: 61  ELKEEARSMFVAAEKHSEKLSLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDL 120
           ELKE ARSMFVAAEKHS+KL LIDS+QCLGLSYHFEDEIN+ILDQIQNSAHVDEED EDL
Sbjct: 61  ELKEAARSMFVAAEKHSDKLCLIDSIQCLGLSYHFEDEINEILDQIQNSAHVDEEDEEDL 120

Query: 121 YIVALKFRLLRQRGFFVSCEMFKKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIM 180
           YI+ALKFRLLRQ+GF VSCE+F KFT+ESGDFKESITKDE+GLLSLYEASHLRMKGE+IM
Sbjct: 121 YIIALKFRLLRQQGFPVSCEIFNKFTTESGDFKESITKDEKGLLSLYEASHLRMKGESIM 180

Query: 181 DEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKN-SN 240
           DEALEFT+TQLEAMAMDSTSPFS+EAKYALKWPIYKTL RFMTR+YISLY NNP KN +N
Sbjct: 181 DEALEFTTTQLEAMAMDSTSPFSQEAKYALKWPIYKTLSRFMTRHYISLYHNNPTKNDNN 240

Query: 241 ILLTFAKLDYNSLQKLYQRELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKY 300
           ILLTFAKLDYNSLQKLYQRELGELSRWWND KLKEKLPFARDRVVESYIWALGIYYEPKY
Sbjct: 241 ILLTFAKLDYNSLQKLYQRELGELSRWWNDSKLKEKLPFARDRVVESYIWALGIYYEPKY 300

Query: 301 SSARTIVAKIIAIITVIDDMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAIL 360
           SSART++AKIIA+IT+IDDMYDCYGTLEELQLFT  I RWD+NCIEELPNYMKVLYEAIL
Sbjct: 301 SSARTLLAKIIALITLIDDMYDCYGTLEELQLFTNTIGRWDMNCIEELPNYMKVLYEAIL 360

Query: 361 EFYEGSIKDMCMDNNIPYAFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSI 420
           + YEG+I+DMCMDNNIPYAFDYAKEGIIRQCKLY+VEAKWFNEDYVPTIEEYMK+SATSI
Sbjct: 361 KIYEGTIQDMCMDNNIPYAFDYAKEGIIRQCKLYMVEAKWFNEDYVPTIEEYMKVSATSI 420

Query: 421 GVYAVASIAFLSLGNIVSEEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCS 480
           G+YAVASIAFLS+GNIVSEE+FQWVQGNPMLH+ASE ASRLVNDIVSHKFG+ERCHVTCS
Sbjct: 421 GLYAVASIAFLSIGNIVSEEIFQWVQGNPMLHKASEVASRLVNDIVSHKFGQERCHVTCS 480

Query: 481 IECYMEQYGVSKEIAVAELKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYK 540
           IECYMEQYGVSKE+A+AELKKQV EA KEIIEDYIKRGKFP VIHD GPNFARVTDFYYK
Sbjct: 481 IECYMEQYGVSKEVAIAELKKQVDEARKEIIEDYIKRGKFPRVIHDDGPNFARVTDFYYK 540

Query: 541 ERDGFTFANTETKHLIALLLTEPVPI 566
           ERDGFTFANTETKHLIAL+LT+P+PI
Sbjct: 541 ERDGFTFANTETKHLIALILTDPMPI 563

BLAST of CSPI02G27020 vs. NCBI nr
Match: XP_008462843.2 (PREDICTED: probable terpene synthase 2 isoform X2 [Cucumis melo])

HSP 1 Score: 1005.0 bits (2597), Expect = 2.6e-289
Identity = 498/566 (87.99%), Postives = 534/566 (94.35%), Query Frame = 0

Query: 1   MSFQITPSESEAANKLTLSDNDVLRRIEYHEPSAWGDYFLSNISNLSILLEEDSVLKKLE 60
           MSFQ   SESEA NKLTLSDN VLRR EYH+PSAWGDYFLSN+SN   +LEEDSVLKKLE
Sbjct: 1   MSFQTIASESEAPNKLTLSDNGVLRRTEYHQPSAWGDYFLSNLSN---ILEEDSVLKKLE 60

Query: 61  ELKEEARSMFVAAEKHSEKLSLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDL 120
           ELKE ARSMFVAAEKHS+KL LIDS+QCLGLSYHFEDEIN+ILDQIQNSAHVDEED EDL
Sbjct: 61  ELKEAARSMFVAAEKHSDKLCLIDSIQCLGLSYHFEDEINEILDQIQNSAHVDEEDEEDL 120

Query: 121 YIVALKFRLLRQRGFFVSCEMFKKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIM 180
           YI+ALKFRLLRQ+      E+F KFT+ESGDFKESITKDE+GLLSLYEASHLRMKGE+IM
Sbjct: 121 YIIALKFRLLRQQ------EIFNKFTTESGDFKESITKDEKGLLSLYEASHLRMKGESIM 180

Query: 181 DEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKN-SN 240
           DEALEFT+TQLEAMAMDSTSPFS+EAKYALKWPIYKTL RFMTR+YISLY NNP KN +N
Sbjct: 181 DEALEFTTTQLEAMAMDSTSPFSQEAKYALKWPIYKTLSRFMTRHYISLYHNNPTKNDNN 240

Query: 241 ILLTFAKLDYNSLQKLYQRELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKY 300
           ILLTFAKLDYNSLQKLYQRELGELSRWWND KLKEKLPFARDRVVESYIWALGIYYEPKY
Sbjct: 241 ILLTFAKLDYNSLQKLYQRELGELSRWWNDSKLKEKLPFARDRVVESYIWALGIYYEPKY 300

Query: 301 SSARTIVAKIIAIITVIDDMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAIL 360
           SSART++AKIIA+IT+IDDMYDCYGTLEELQLFT  I RWD+NCIEELPNYMKVLYEAIL
Sbjct: 301 SSARTLLAKIIALITLIDDMYDCYGTLEELQLFTNTIGRWDMNCIEELPNYMKVLYEAIL 360

Query: 361 EFYEGSIKDMCMDNNIPYAFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSI 420
           + YEG+I+DMCMDNNIPYAFDYAKEGIIRQCKLY+VEAKWFNEDYVPTIEEYMK+SATSI
Sbjct: 361 KIYEGTIQDMCMDNNIPYAFDYAKEGIIRQCKLYMVEAKWFNEDYVPTIEEYMKVSATSI 420

Query: 421 GVYAVASIAFLSLGNIVSEEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCS 480
           G+YAVASIAFLS+GNIVSEE+FQWVQGNPMLH+ASE ASRLVNDIVSHKFG+ERCHVTCS
Sbjct: 421 GLYAVASIAFLSIGNIVSEEIFQWVQGNPMLHKASEVASRLVNDIVSHKFGQERCHVTCS 480

Query: 481 IECYMEQYGVSKEIAVAELKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYK 540
           IECYMEQYGVSKE+A+AELKKQV EA KEIIEDYIKRGKFP VIHDYGPNFARVTDFYYK
Sbjct: 481 IECYMEQYGVSKEVAIAELKKQVDEARKEIIEDYIKRGKFPRVIHDYGPNFARVTDFYYK 540

Query: 541 ERDGFTFANTETKHLIALLLTEPVPI 566
           ERDGFTFANTETKHLIAL+LT+P+PI
Sbjct: 541 ERDGFTFANTETKHLIALILTDPMPI 557

BLAST of CSPI02G27020 vs. TAIR 10
Match: AT5G23960.2 (terpene synthase 21 )

HSP 1 Score: 347.8 bits (891), Expect = 1.6e-95
Identity = 188/521 (36.08%), Postives = 313/521 (60.08%), Query Frame = 0

Query: 50  LEEDSVLKKLEELKEEARSMFVAAEKHS-EKLSLIDSLQCLGLSYHFE----DEINKILD 109
           L  ++  +K   LKE  +  F++++ +  E +  ID+L  LG+SYHFE    ++++K  D
Sbjct: 29  LGTETFKEKHSTLKEAVKEAFMSSKANPIENIKFIDALCRLGVSYHFEKDIVEQLDKSFD 88

Query: 110 QIQNSAHVDEEDVEDLYIVALKFRLLRQRGFFVSCEMFKKFTSESGDFKESITKDERGLL 169
            +     V +E   DLY V + F++ RQ GF +S ++F+KF  E+G FK  +  D  G+L
Sbjct: 89  CLDFPQMVRQEGC-DLYTVGIIFQVFRQFGFKLSADVFEKFKDENGKFKGHLVTDAYGML 148

Query: 170 SLYEASHLRMKGENIMDEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTR 229
           SLYEA+     GE+I+DEAL F+ + LE ++  S+   +   K ALK P +K + R  TR
Sbjct: 149 SLYEAAQWGTHGEDIIDEALAFSRSHLEEISSRSSPHLAIRIKNALKHPYHKGISRIETR 208

Query: 230 NYISLYRNNPLKNSNILLTFAKLDYNSLQKLYQRELGELSRWWNDLKLKEKLPFARDRVV 289
            YIS Y      +   LL FAK+D+N LQ L++ EL  ++RW ++++ K K+ + R R+ 
Sbjct: 209 QYISYYEEEESCDPT-LLEFAKIDFNLLQILHREELACVTRWHHEMEFKSKVTYTRHRIT 268

Query: 290 ESYIWALGIYYEPKYSSARTIVAKIIAIITVIDDMYDCYGTLEELQLFTKAIERWDLNCI 349
           E+Y+W+LG Y+EP+YS AR I    + + T +DDMYD YGT+EEL+LFT A++ + +  I
Sbjct: 269 EAYLWSLGTYFEPQYSQARVITTMALILFTALDDMYDAYGTMEELELFTDAMDEYSICNI 328

Query: 350 EELPNYMKVLYEAILEFYEGSIKDMCMDNNIPYAFDYAKEGIIRQCKLYIVEAKWFNEDY 409
             +P+ MK +Y   +EFY+   +++  +      F + K+ + +    Y+ EAKW  +DY
Sbjct: 329 IPIPDSMKFIYNVTVEFYDKLDEELEKEGRSGCGF-HLKKSLQKTANGYMQEAKWLKKDY 388

Query: 410 VPTIEEYMKISATSIGVYAVASIAFLSLGNIVSEEVFQWVQGNPMLHQASEAASRLVNDI 469
           + T +EY + +  S G YA+ ++ F+ + ++   + F+W+  +P +  ASE  SR  +DI
Sbjct: 389 IATFDEYKENAILSSGYYALIAMTFVRMTDVAKLDAFEWLSSHPKIRVASEIISRFTDDI 448

Query: 470 VSHKFGEERCHVTCSIECYMEQYGVSKEIAVAELKKQVGEAWKEIIEDYIKRGKFPSVIH 529
            S++F  +R HV   I+CYM+Q+GVSKE AV  +   V +AWK++ ++ ++   FP  + 
Sbjct: 449 SSYEFEHKREHVATGIDCYMQQFGVSKERAVEVMGNIVSDAWKDLNQELMRPHVFPFPLL 508

Query: 530 DYGPNFARVTDFYYKERDGFTFANTETKHLIALLLTEPVPI 566
               N +RV D +Y+ +D +T      +H+++LL+ E +PI
Sbjct: 509 MRVLNLSRVIDVFYRYQDAYTNPKLLKEHIVSLLI-ETIPI 545

BLAST of CSPI02G27020 vs. TAIR 10
Match: AT5G23960.1 (terpene synthase 21 )

HSP 1 Score: 345.1 bits (884), Expect = 1.0e-94
Identity = 189/523 (36.14%), Postives = 313/523 (59.85%), Query Frame = 0

Query: 50  LEEDSVLKKLEELKEEARSMFVAAEKHS-EKLSLIDSLQCLGLSYHFE----DEINKILD 109
           L  ++  +K   LKE  +  F++++ +  E +  ID+L  LG+SYHFE    ++++K  D
Sbjct: 29  LGTETFKEKHSTLKEAVKEAFMSSKANPIENIKFIDALCRLGVSYHFEKDIVEQLDKSFD 88

Query: 110 QIQNSAHVDEEDVEDLYIVALKFRLLRQRGFFVSCEMFKKFTSESGDFKESITKDERGLL 169
            +     V +E   DLY V + F++ RQ GF +S ++F+KF  E+G FK  +  D  G+L
Sbjct: 89  CLDFPQMVRQEGC-DLYTVGIIFQVFRQFGFKLSADVFEKFKDENGKFKGHLVTDAYGML 148

Query: 170 SLYEASHLRMKGENIMDEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTR 229
           SLYEA+     GE+I+DEAL F+ + LE ++  S+   +   K ALK P +K + R  TR
Sbjct: 149 SLYEAAQWGTHGEDIIDEALAFSRSHLEEISSRSSPHLAIRIKNALKHPYHKGISRIETR 208

Query: 230 NYISLYRNNPLKNSNILLTFAKLDYNSLQKLYQRELGELSRWWNDLKLKEKLPFARDRVV 289
            YIS Y      +   LL FAK+D+N LQ L++ EL  ++RW ++++ K K+ + R R+ 
Sbjct: 209 QYISYYEEEESCDPT-LLEFAKIDFNLLQILHREELACVTRWHHEMEFKSKVTYTRHRIT 268

Query: 290 ESYIWALGIYYEPKYSSARTIVAKIIAIITVIDDMYDCYGTLEELQLFTKAIERWDLNCI 349
           E+Y+W+LG Y+EP+YS AR I    + + T +DDMYD YGT+EEL+LFT A++ W     
Sbjct: 269 EAYLWSLGTYFEPQYSQARVITTMALILFTALDDMYDAYGTMEELELFTDAMDEWLPVVP 328

Query: 350 EE--LPNYMKVLYEAILEFYEGSIKDMCMDNNIPYAFDYAKEGIIRQCKLYIVEAKWFNE 409
           +E  +P+ MK +Y   +EFY+   +++  +      F + K+ + +    Y+ EAKW  +
Sbjct: 329 DEIPIPDSMKFIYNVTVEFYDKLDEELEKEGRSGCGF-HLKKSLQKTANGYMQEAKWLKK 388

Query: 410 DYVPTIEEYMKISATSIGVYAVASIAFLSLGNIVSEEVFQWVQGNPMLHQASEAASRLVN 469
           DY+ T +EY + +  S G YA+ ++ F+ + ++   + F+W+  +P +  ASE  SR  +
Sbjct: 389 DYIATFDEYKENAILSSGYYALIAMTFVRMTDVAKLDAFEWLSSHPKIRVASEIISRFTD 448

Query: 470 DIVSHKFGEERCHVTCSIECYMEQYGVSKEIAVAELKKQVGEAWKEIIEDYIKRGKFPSV 529
           DI S++F  +R HV   I+CYM+Q+GVSKE AV  +   V +AWK++ ++ ++   FP  
Sbjct: 449 DISSYEFEHKREHVATGIDCYMQQFGVSKERAVEVMGNIVSDAWKDLNQELMRPHVFPFP 508

Query: 530 IHDYGPNFARVTDFYYKERDGFTFANTETKHLIALLLTEPVPI 566
           +     N +RV D +Y+ +D +T      +H+++LL+ E +PI
Sbjct: 509 LLMRVLNLSRVIDVFYRYQDAYTNPKLLKEHIVSLLI-ETIPI 547

BLAST of CSPI02G27020 vs. TAIR 10
Match: AT3G29410.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 337.0 bits (863), Expect = 2.9e-92
Identity = 203/557 (36.45%), Postives = 307/557 (55.12%), Query Frame = 0

Query: 17  TLSDNDVLRRIEYHEPSAWGDYFLSNISNLSILLEEDSVLKKLEE--LKEEARSMFVAA- 76
           T  D +  R + Y  PS WGD+FLS    +SI   E   L+K  E   K + R M ++  
Sbjct: 56  TSGDLESTRPLTYFSPSYWGDHFLS----VSIDDSEFEALEKEIETVFKPKVRDMLMSPH 115

Query: 77  EKHSEKLSLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDLYIVALKFRLLRQR 136
               E++ LI  L  LG++Y++E+EI +IL +          D +DL  +A+ F + R  
Sbjct: 116 SSDKERIRLIHLLISLGIAYYYENEIEEILHKAYGKLACLISDEDDLETIAIMFEVFRLY 175

Query: 137 GFFVSCEMFKKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIMDEALEFTSTQLEA 196
           G  + C++F++F SE G FKES+  D RGLL LYEA+HL    E+IMDEAL F    LE 
Sbjct: 176 GHKMPCDVFERFKSEDGKFKESLVGDVRGLLQLYEAAHLGAPSEDIMDEALSFARYHLEP 235

Query: 197 MA-MDSTSPFSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKNSNILLTFAKLDYNSL 256
           +A  +++S   +  +  L    Y ++   + R YIS Y +        LL F+KL++N  
Sbjct: 236 LAGTETSSNLFKHVENVLYRARYHSIEILVARQYISFY-DQEEDQDETLLRFSKLNFNFC 295

Query: 257 QKLYQRELGELSRWWNDLKLKEKLPFA-RDRVVESYIWALGIYYEPKYSSARTIVAKIIA 316
           Q  Y +EL  ++RWW +L +  KLP++ R+R VE+Y+  LG+ +EP+YS AR  +AK+  
Sbjct: 296 QMHYVKELKIVTRWWKELGIASKLPYSIRERNVETYLGGLGVLFEPRYSLARIFLAKLTL 355

Query: 317 IITVIDDMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAILEFYEGSIKDMC- 376
           I+TV+DD  D Y TL E+Q    A  RWDL  +EELP YM+++Y+++ E  E   ++M  
Sbjct: 356 IMTVVDDTCDAYATLPEVQSLHDAFHRWDLRAMEELPRYMRIIYQSVFETVEDIDREMIA 415

Query: 377 --MDNNIPYAFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSIGVYAVASIA 436
                 +    D  K  +I     Y+  AKW   D VP+ E+YM+I   S  +   AS  
Sbjct: 416 RGKHGRLQLTIDEIKSLMI----WYLGIAKWARSDQVPSFEDYMEIGTPSSALDDFASYG 475

Query: 437 FLSLGNIVSEEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCSIECYMEQYG 496
           F+++ +   +++ +W    P +  A  A  R+ NDIV+ +    R  V   + CYM+Q+G
Sbjct: 476 FIAMDDCDQKQLKEWFYSKPKIFHALNALFRIRNDIVTFEQEMSRGEVANGVNCYMKQHG 535

Query: 497 VSKEIAVAELKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYKERDGFTFAN 556
           V+KE AV EL+K   E++K +IE+++     P  I     N ARV D +YKE DGF   +
Sbjct: 536 VTKEAAVEELRKMERESYKIMIEEFMTSKAMPRQILVRPVNIARVMDLFYKEADGFGHPD 595

Query: 557 TETKHLIALLLTEPVPI 566
            +   LIA L   P+P+
Sbjct: 596 QKLLQLIASLFLHPIPL 603

BLAST of CSPI02G27020 vs. TAIR 10
Match: AT3G14490.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 318.9 bits (816), Expect = 8.1e-87
Identity = 197/549 (35.88%), Postives = 299/549 (54.46%), Query Frame = 0

Query: 25  RRIEYHEPSAWGDYFLSNISNLSILLEEDSVLKKLE-ELKEEARSMFVAAE-KHSEKLSL 84
           R + +  P+ WGD+FLS   +++   E D   +++E  +K + R M  +++   +E++ L
Sbjct: 60  RPLAHFSPTLWGDHFLSVPLHVA---EFDDFSREIEVTMKPKVRDMLKSSKNSDNERIRL 119

Query: 85  IDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDLYIVALKFRLLRQRGFFVSCEMF 144
           I  L  LG++YHFE EI++IL Q   +         DL  ++  F + R RG+++ C  F
Sbjct: 120 IHLLMNLGIAYHFEIEIDEILGQAFGNLDDIIAKENDLETISTMFEVFRLRGYYMPCYAF 179

Query: 145 KKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIMDEALEFTSTQLEAMAMDST--- 204
            +F  E G FKES+ +D RG+L LYEA+HL    E+IMDEAL FT  +LE++  + T   
Sbjct: 180 NRFKGEDGRFKESLAEDIRGMLQLYEAAHLGTPSEDIMDEALSFTRYRLESLTSNHTATA 239

Query: 205 SP-FSEEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKNSNILLTFAKLDYNSLQKLYQR 264
           SP  S+  + AL    Y  L   + R YIS Y      +   LL FAKL++N  Q  Y +
Sbjct: 240 SPHLSKHIQNALYRARYHNLEILVAREYISFYEQEE-DHDETLLKFAKLNFNYCQLHYIQ 299

Query: 265 ELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKYSSARTIVAKIIAIITVIDD 324
           EL +L++WW +L L  KLP+ RDR+VE Y  AL +Y+EP+YS  R IV KI  I+TV +D
Sbjct: 300 ELKDLTKWWKELDLASKLPYIRDRIVEVYFGALALYFEPRYSLGRIIVTKITMIVTVFND 359

Query: 325 MYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAILEFYEGSIKDMCMDNNIPYA 384
             D YGTL E+     + +RWDL  IE+LP+Y+K+++  + E  E  I+           
Sbjct: 360 TCDAYGTLPEVTSLVDSFQRWDLGDIEKLPSYVKIVFRGVFETLE-EIEQEMRPQGRSRI 419

Query: 385 FDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKIS-ATSIGVYAVASIAFLSLGNIVS 444
              A + I +  K Y+  +KW    +VPT EEYM+    TS+  +A  S  F+++ +   
Sbjct: 420 VQVAVDEIKKLGKAYLAISKWARASHVPTFEEYMEFGMQTSMDHFAAYS--FIAMEDCDE 479

Query: 445 EEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCSIECYMEQYGVSKEIAVAE 504
            +  +W +  P + +A     R+ NDI + +    R  V   + CYM+Q+GVSKE A+ E
Sbjct: 480 NQTCEWYKSRPKMMEALNGVFRIKNDINTFEQEMSRGEVAKGLNCYMKQHGVSKEEAIGE 539

Query: 505 LKKQVGEAWKEIIEDYIKRGKFPSVIHDYGPNFARVTDFYYKERDGFTFAN-TETKHLIA 564
           + K     +K I+E+Y+     P  I     N +R    +YKERD FT       K +I 
Sbjct: 540 MNKIYSNYYKIIMEEYLTTTAVPRPILVRCLNVSRPIHHFYKERDEFTDPYFGMLKEVIT 599

Query: 565 LLLTEPVPI 566
            L   P+P+
Sbjct: 600 SLFIHPIPL 601

BLAST of CSPI02G27020 vs. TAIR 10
Match: AT3G14540.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 315.1 bits (806), Expect = 1.2e-85
Identity = 188/552 (34.06%), Postives = 293/552 (53.08%), Query Frame = 0

Query: 20  DNDVLRRIEYHEPSAWGDYFLSNISNLSILLEEDSVLKKLEELKEEARSMFVAAE-KHSE 79
           D +  R   Y  PS WGD+FLS   + +  +E +   +++E +K   + M ++++    E
Sbjct: 55  DLETSRPSTYFSPSLWGDHFLSVSLDHAEFVELE---REIETMKPLVKDMLMSSQSSDKE 114

Query: 80  KLSLIDSLQCLGLSYHFEDEINKILDQIQNSAHVDEEDVEDLYIVALKFRLLRQRGFFVS 139
           K+ LI  L  LG SYHF+ EI  IL            + +DL  +++ F + R  G  +S
Sbjct: 115 KIRLIHLLVSLGSSYHFDKEIQDILKHSFTKLDGIIVEEDDLETISIMFEVFRLYGHKMS 174

Query: 140 CEMFKKFTSESGDFKESITKDERGLLSLYEASHLRMKGENIMDEALEFTSTQLEAMAMDS 199
           C+ F +F    G FKES+ KD RG+L L+E +HL    E+IMDEAL FT   LE++   +
Sbjct: 175 CDAFDRFRGGDGRFKESLAKDVRGMLQLFEVAHLGTLSEDIMDEALRFTRNHLESLTSGN 234

Query: 200 TSPFS----EEAKYALKWPIYKTLPRFMTRNYISLYRNNPLKNSNILLTFAKLDYNSLQK 259
            S  S    +  + +L  P Y  +   + R YIS Y      N  ILL FAKL++N  Q 
Sbjct: 235 VSSASPHILKHIQNSLYIPRYCNIEVLVAREYISYYEQEEGYN-EILLKFAKLNFNFCQC 294

Query: 260 LYQRELGELSRWWNDLKLKEKLPFARDRVVESYIWALGIYYEPKYSSARTIVAKIIAIIT 319
            Y +E+  L++WW DL L  KLP+ RDR VES++  LG Y+EP+YS  R IVAK I II 
Sbjct: 295 HYIQEIKTLTKWWKDLDLASKLPYIRDRSVESHLGGLGPYFEPQYSLGRIIVAKTIMIIV 354

Query: 320 VIDDMYDCYGTLEELQLFTKAIERWDLNCIEELPNYMKVLYEAILEFYEGSIKDMCMDNN 379
           V DD YD + T+ E  + T+  +R ++   ++L  Y++++ E++ E   G I+       
Sbjct: 355 VADDTYDAHATIPEATVLTEYFQRLNIGADDKLSGYLRIVLESVFEVM-GEIEQEMSPKG 414

Query: 380 IPYAFDYAKEGIIRQCKLYIVEAKWFNEDYVPTIEEYMKISATSIGVYAVASIAFLSLGN 439
             Y+     E      K Y    +W  + +VPT +EYMK+   + G+   A   F+ + +
Sbjct: 415 RSYSVKQVLERFKIIAKAYKQLTEWARKGHVPTFDEYMKVGLVTAGMGDYAGYCFIGMED 474

Query: 440 IVSEEVFQWVQGNPMLHQASEAASRLVNDIVSHKFGEERCHVTCSIECYMEQYGVSKEIA 499
           I  +E F+W+  NP+L  A     R+ ND+ +++    R  V   + CYM+QYGV+KE A
Sbjct: 475 INEKEAFEWLNSNPLLIDALNVLFRIANDVGTYETEINRGEVANGLNCYMKQYGVTKEEA 534

Query: 500 VAELKKQVGEAWKEIIEDYI-KRGKFPSVIHDYGPNFARVTDFYYKERDGFTFANTETKH 559
             EL+K      K ++E+++    + P  +     NFAR+ D  Y E DG++    + +H
Sbjct: 535 SRELRKMYIYNKKVVVEEFMNSHDRVPRQVLLRCLNFARLFDVIYTEGDGYSEPKGKIEH 594

Query: 560 LIALLLTEPVPI 566
            +  L   P+P+
Sbjct: 595 FMTSLYVHPIPL 601

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6Q3H34.2e-13344.59(-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 S... [more]
B9SCB63.0e-13143.87Probable terpene synthase 2 OS=Ricinus communis OX=3988 GN=TPS2 PE=3 SV=1[more]
B2KSJ57.5e-13045.13(+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1[more]
P936652.0e-12743.07(+)-delta-cadinene synthase OS=Gossypium hirsutum OX=3635 GN=CDN1 PE=1 SV=1[more]
Q397602.0e-12743.25(+)-delta-cadinene synthase isozyme XC14 OS=Gossypium arboreum OX=29729 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LN250.0e+0099.29Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G427840 PE=4 SV=1[more]
A0A1S3CHV01.7e-29488.87probable terpene synthase 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501122 P... [more]
A0A5D3BQ162.9e-29488.38Putative terpene synthase 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A5A7VDK32.5e-29388.69Putative terpene synthase 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CHV81.3e-28987.99probable terpene synthase 2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103501122 P... [more]
Match NameE-valueIdentityDescription
XP_004151916.20.0e+0099.29(-)-germacrene D synthase [Cucumis sativus] >KGN63310.1 hypothetical protein Csa... [more]
XP_008462842.23.5e-29488.87PREDICTED: probable terpene synthase 2 isoform X1 [Cucumis melo][more]
TYK00862.16.0e-29488.38putative terpene synthase 2 isoform X1 [Cucumis melo var. makuwa][more]
KAA0066422.15.1e-29388.69putative terpene synthase 2 isoform X1 [Cucumis melo var. makuwa][more]
XP_008462843.22.6e-28987.99PREDICTED: probable terpene synthase 2 isoform X2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT5G23960.21.6e-9536.08terpene synthase 21 [more]
AT5G23960.11.0e-9436.14terpene synthase 21 [more]
AT3G29410.12.9e-9236.45Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
AT3G14490.18.1e-8735.88Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
AT3G14540.11.2e-8534.06Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 56..79
NoneNo IPR availableSFLDSFLDS00005Isoprenoid_Synthase_Type_Icoord: 239..563
e-value: 0.0
score: 337.8
NoneNo IPR availablePANTHERPTHR31225OS04G0344100 PROTEIN-RELATEDcoord: 21..546
NoneNo IPR availablePANTHERPTHR31225:SF111TERPENE SYNTHASE 2-RELATEDcoord: 21..546
IPR036965Terpene synthase, N-terminal domain superfamilyGENE3D1.50.10.130coord: 75..236
e-value: 6.8E-185
score: 617.3
IPR005630Terpene synthase, metal-binding domainPFAMPF03936Terpene_synth_Ccoord: 242..508
e-value: 1.7E-97
score: 326.0
IPR001906Terpene synthase, N-terminal domainPFAMPF01397Terpene_synthcoord: 35..210
e-value: 1.1E-51
score: 175.2
IPR008949Isoprenoid synthase domain superfamilyGENE3D1.10.600.10Farnesyl Diphosphate Synthasecoord: 32..553
e-value: 6.8E-185
score: 617.3
IPR008949Isoprenoid synthase domain superfamilySUPERFAMILY48576Terpenoid synthasescoord: 239..563
IPR034741Terpene cyclase-like 1, C-terminal domainSFLDSFLDG01019Terpene_Cyclase_Like_1_C_Termcoord: 239..563
e-value: 0.0
score: 337.8
IPR044814Terpene cyclases, class 1, plantCDDcd00684Terpene_cyclase_plant_C1coord: 25..562
e-value: 0.0
score: 583.773
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidSUPERFAMILY48239Terpenoid cyclases/Protein prenyltransferasescoord: 32..233

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G27020.1CSPI02G27020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016102 diterpenoid biosynthetic process
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0043565 sequence-specific DNA binding
molecular_function GO:0010333 terpene synthase activity
molecular_function GO:0016829 lyase activity