CSPI02G24770 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G24770
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptioneukaryotic translation initiation factor 2D
LocationChr2: 21249598 .. 21255318 (+)
RNA-Seq ExpressionCSPI02G24770
SyntenyCSPI02G24770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAAAGAATCACTGTTTAGGTATATCTCATCACCGTTCCAGAACTATAGCGTCTCCGGAACTCATTAGAAAGGCTAAAGAACCTGGACGAAACCTTTGCACTGCACTTGCTTCCCCTCTCCTAAAGTAACATTGACGTCTCCTTCTCCCACAATCATCCCATCGCTCACGTCTACGGCCACGCTCCTCTCTCCCCCGCTGCGTTCATCACTCTCCATCGTCCTCCAGCTCCAGCGAGTAAACGATTTAGGAACAATATGTTCAAGAAGGCGTCAGAGGCGAAGTTGCAGCAGAGATTATCTGGTGCAGACAGGAAGAAACTGAAGAGGACTGTTAAAGAAAGGTTCCCTGCAGCTTCCGATGCCGATATTGATCTCTTGCTTCCTCCCAAGGTATTGCTATTTTCCAATCAATTTTGTCTGTCCCAATTTAACAATGGCAACAGCTTTCCAGATTACGTTGAGACTTTTTTTTTAATCGTTTTTTGTTTAAGTTACCGCTGAACATCACTTGTGTATAAGATCAATCCTGCACTCTAATTCTTATCTACCTGAAAAAAAGTTGTCTTGGTACTAGCATTGCGATGAACCTTGCGTGGGTAGCTACCAAACTAAACATAATTTTTTCATGCCTTCAATATTTACTCATTTCGTGGAGGAGGAGAATTTGTCACTAGATAGCTCAATTTTAAAGAAATGGCAGCAGATTGAGTAAAAGAGTGAAGAGGACCTTTGGGAGATTATGGTCTTCTTGACTTATTGGTATATTTTGTCAAATCTCCTTTGCAAGTATTATATTTATTCCATCACTGCCAACCATAGAGTTCACTTTTGTTTTCTTTAGTAATCATCATCTACCCCTGTACTTCATTCTATAAATCCTAAATTAATATGTTTTAGGTCGAGTTTTCTGTTGCCAAGTTCCAAAATCGCGTTCACATATATAGCGTTGAAGGAGGCTTTCCAATGTTTTTCGACGTTGATGGGAGAGGAAATGAAATATTCCCCACAGGTATTTGATCCTAAGAATCTATTAAAAATTTCACCACTGCGATTAGTTATTGTGAGGGTTCCTAAAGCTTTCCTTTGCAGAATTTTAGTTATTTTTGGATTGAACGATTGTCTTTCAGTCTTATTGGTTTTAGTAACATTCTCAATTCCATTGTAGTTTTTGCACTCTGGATGGTACCGGAATTGTTGCCTGCCTTTTTTTTGAAGGGGGGTGAAGTCTCTCGCTTTGTGATTGGAGGAGCAGACTTGATGTTCCCTGGTATTCACGTTCCTGCTGAAGGTCTTCCTTCATTTTTATCTGGGGAACCATGGGCTGTGAAAGTCCCTGGCAATGCATCTCCTATTGCTGTAAGTTTCACTGAAATTTGATCTTATGTTGCAATTTAAATTTGGCTGTGAAGACAAGTTAGCACTCTATTCAACCTGAAGATCTCATGCAGGTTGGTGTTACCACCATGAGTAGCACCGAAGCACTAAAAGCAGGTTTACGTGGAAAGGCTCTGAGAATTCTACACTACTACAGGGATACCCTTTGGTGAGATTGATCAAATTGGTTTAGAAACAGAGTGATCAATTATCGATGTGTGACACGATTTTCTCTCTTGAATTTATTTTTCAGGGGATTGGTAGAGGGTCATTATGTGCCCAATGCAGGATTTTTGGAAGATGCTGTATATGGAGATCCTGCTTTGGCATCATCTTCACAAATTGATGACTCTTATGAAGGTACTGAAACTGGTGATGCTACAAATTATGGACAGAATGACAGCGGCAATAAGGAAGTTGGGGGATCTGTTGATGAAACTACAACCGATGTTCTTGATGAGCCTGGCGTTGTTCCAACAGCACAGACAGATTCAACAACTGATGAAATCGTTTCTGGTGTGAATGATTTGAAGGTTGTAAATGATATTTCCACTGAGGAACCAAATGTGCAGCATACTCTATCAGCTGAGGACGTGGACAAGCTTCTGGATAAATGTCTTCTTCAAGCCCTGCACATAACAGTGAAAGACAAAGATTTTCCACTTCCTGGAAGCACGCTATGGTAAGAAATTCTGACCTGTCATAAAATTCTGACATAGGAGGGCTTTGTGTTGCTTCCCTCTGAAGGCTTAAGTTTTTAATATATTCCATGTAGTTTAATCATATGGAATATTTGATACAAATTGACAATTCTTTCACGTTCTAAGTGTTATTCTAAATTTATGTAGTCTAGGGTAGTAGTCTAGGGAATCAAACATCCATCGTTACCAGTGTTGGATTCATATCGTAGCTCGCAGCACTAGGAACATCTGTGTTTCTGACTGAATCTTCTTCTTGGCTGTCGGCCCTTTAGTATATCTTCTATGAAATTTGACTTTTTTTTAAACTATCTGTGGAAGAACTAATCTTTTATATTAAAAGAACTGTACTGACATAGAAGTAAGACAGACCACAATACTGGAAAAGTGGAAAAGAAATGATATAATACAACTTGAAAACCAAATCTAGGGGTGGTGTGTGTGGGAGAAAAAAGGGGGGTGGGCAGAGGGTGGAACATCCTCAAAATGTCTGTTGATTGATAGAAAAGCTCTTGACTTGAATGCTTACTATTGTGAATTTGAAGACCCATTGTTTAATTTTCTAGCAGATAACTATATTTCATTCACTTTTTGCCTGATGTAGGTCAAATCATGTATTGCCTTGTCGGCCTCCTGGGATGATGCTAGACATCAAGAAATCTTCCTACAAGAAATTGTCTAAGTGGTTGCAAGCTAAATCCTCCGCTGGACTGGTAATGCTACTTTTATTCAGCTTCTTAGTTTCAAAATGAACTGCTGACTTTGTATCAAACTTAAAACCTCATAGTCATGACCAAGCAAGACGGAATAAAACCACGCATAGTTCATCTTTATGCTTTGCTAGTTGATTTTTTAGAACAAGCAATGTCGTCTTCTTTGTCTTTGCCTTTTTAGTAAGAAATGGGTAGGTATATAGATTGATATTAGTGTAAAAATCAGGCGTATTGTATATAGATTGAAAAATAGGCATGAAGTTCAATTTATAATTCAGTTATTTTTATGATAGGGTTCAAATCTTAGTGACCACGTACATAAGGAGGCTTGTTCATGTGCTTCTTAATGATAAATGTTAGTATAGACAACAACCACCATTGCAATGAAAATTCCTATTTGTTGATTTTATTCATCATGGAGGCAAAATATAACTAACCAAATTCGAGTACCTTTTGGAGTTCTTCACTAGGATTGGAATTGGTCCCCCCCCCACTTTTTATTTACCATTTTATGCTGAACAGAATTTGTTTTTTATCAGTAGAAAAAAAGGTAGAGAGAGAATAAATGATGGAAAGAGTTTCCAATTTATATCCTCCCCTACAGAGGCAAAGTATTTAAAAAACAATGCTATCTCAAACTTTGTTCTGCCTTTATATGTCTCCTCAAACAAAGTACGCACAATTTTCAGTTTACTATCGGATGAAGGCTGGTTTTTGAAGTTGGATACATTACCTGAAGATCTTGATTCCTATCAGGGAAAAACAACAAAAATGAAATGCTTACTTATATCAGTTAATGTGCAAATGTGCATTGAATATATTGTTTATTTGTTTCAGATTACAGTGAAAGAAGACAAGCATAAAAAAGAGACGGTTTTGTACTCTGTTAACCGTAAGCATCCAGACTATACTTCCTTTAAACCAGAGAAGCAACAGGTAGAGAAAATTGATCAGAGCAATAATCATCACGGTCTGAAAGAAAGTTCATCCAATAAAATGCTTGAAGTAGCAGAGATATACAAGCCAAGTGTGCATGTCAATCCCATTTTTGTTGCTGTGGGGGCAGACACTAGCAAACTTTACACTTCAGCAGAGGCTACTGATATTGTCTTCCAATACGTCGAGAAAGAAAAATTGGAGAAACCAACAGATAAGTCTAGAGTGGTTTTGGATGCAATATTATGCGATTCACTGTTCAAGGGGGCCATTAAGAAAGGAACAACGTATCCTACAGAAATTCACAAAAGAGATATAGGCTCAACATTTATCAGCCGGATGCAACCTCATCACATTGTAACAAGAGGAAGTGAGTCTGTCGTTCGCAAAGGTGCACTAAAAACTATTCAAATAATGACAGAGCGACGCCAAGGAAATAAGAAAGTGACAAGACTTTCTGGGTTGGAAACGTTCTTGTTGGATGCTGAAGCATTGGCTTCAGAGCTTCAGAAAAAGTGTGCTTGCAGTACGACGGTGGCAGAGTTGCCTGGTAATATAACCTGTCTACATATCTTTTGTTCTTTTGTTTTTTTTGTTTTTTCTCAAAAGTAACAATTTTGAACGGTGGTATGAAATTACAAATGAGGAGTTAAATGAGACTTTTCTAATAGGAAAAGAGAGTGGAAAAGCTAGAGTTGTTTGTTTTTGCAGTAACATTATTTCTGACATCAAGGGAAATTCCCATGGATATCAGCAATTTTCATTTCAACATTTGGAAATGAATAAGTTACGAATTGCGGAGTATGGAAGGCTGTACTTTCTTCTTCTTTTCCTTTTGTTTCCAACTTTTTCTCGTTTCTTGATGGGGAAATTTTTTAAATGATGTTAGCAAATTTTCAATTCTTTTGGTTAAGATATTAACTTTCTAAGGATAGGATGCCAAAATTCCGAATGAACACAACATTCATTTGATAATTTTTATTGTTCTCTTTCTGAATATATGGATTCTCCTGATGCATTTTGTAAAAACAAATTCAGGTAAAAAAGGGTATGAAGTTGTGGTCCAAGGTGGAGTCATTGATGACTTGGCAAGACATCTGATCGAACAATATGGAATTCCAAAAAAGTACATTGAAGTACTTGATAAAACAAGGAAATGAGAGGCTAGAGCTGGTTAGTTGGTCTTGATGCTTACTGCTAATTTTGATGTATGTAGTCTTGTTAAAGAATAGTTGGTATTACCTTTAATCTTTCTTTCTCATTAATATTAAATGAACGGCCACCTTACCTCTTTTTGCTTCTTTCCATTTGTGAAGTAACGTTGATATGCTATATTATTCATCGACTGCCTTCCCAGTCTGATTTACCATGGAAATGACCACATGGGTCAACTTGATCTCATCACTGAATTGCATTGCATCAACACGATACAATTTCTACACATGTCCAAGCTACTACCAATTATTATAAACCAGAGTCTTATGGGACCGATCATGTTAAGAACTTAAGATTTAGAAAGGGGAATTAATAGAAAAAGAGGTTGTTAGTATTGTTGACTATTAACTATGTGTACACGGATATGTTAAAAACGTATATTCCATTATTATACTTTTGCTGTTGACTATTATATGCTGGCTGTGTATTGAAGCCTAAGCAGTTTGAGTTAATATTGACACTTGTTTTTACTTTCAGCTATATATCTTACAGGGAACTTACCAATCCCAGTCTTATGATATCAAGTCAAGATCTGCTGTTATCAGCGCCAGCATCATGCAAAGGAAAAAGCCTGAATCAAAAGATATGATAGAAGTTATTGATAATGAGGTATCAAAATTTGATTCATGCAACAAAGTAAAAAGAAGATTCCTCATTCGTCTCTTTAGGCTGGAAAATTGGTTCTAGTTTCATTGACACTGATTGTTTATTTACCTAAAATTTGTTCATCACGTGTTTTCGGCTGCCTTGTGTAAAAGAAAATGTTTGTAGTTAGGACAAACC

mRNA sequence

GAAAAAGAATCACTGTTTAGGTATATCTCATCACCGTTCCAGAACTATAGCGTCTCCGGAACTCATTAGAAAGGCTAAAGAACCTGGACGAAACCTTTGCACTGCACTTGCTTCCCCTCTCCTAAAGTAACATTGACGTCTCCTTCTCCCACAATCATCCCATCGCTCACGTCTACGGCCACGCTCCTCTCTCCCCCGCTGCGTTCATCACTCTCCATCGTCCTCCAGCTCCAGCGAGTAAACGATTTAGGAACAATATGTTCAAGAAGGCGTCAGAGGCGAAGTTGCAGCAGAGATTATCTGGTGCAGACAGGAAGAAACTGAAGAGGACTGTTAAAGAAAGGTTCCCTGCAGCTTCCGATGCCGATATTGATCTCTTGCTTCCTCCCAAGGTCGAGTTTTCTGTTGCCAAGTTCCAAAATCGCGTTCACATATATAGCGTTGAAGGAGGCTTTCCAATGTTTTTCGACGTTGATGGGAGAGGAAATGAAATATTCCCCACAGTTTTTGCACTCTGGATGGTACCGGAATTGTTGCCTGCCTTTTTTTTGAAGGGGGGTGAAGTCTCTCGCTTTGTGATTGGAGGAGCAGACTTGATGTTCCCTGGTATTCACGTTCCTGCTGAAGGTCTTCCTTCATTTTTATCTGGGGAACCATGGGCTGTGAAAGTCCCTGGCAATGCATCTCCTATTGCTGTTGGTGTTACCACCATGAGTAGCACCGAAGCACTAAAAGCAGGTTTACGTGGAAAGGCTCTGAGAATTCTACACTACTACAGGGATACCCTTTGGGGATTGGTAGAGGGTCATTATGTGCCCAATGCAGGATTTTTGGAAGATGCTGTATATGGAGATCCTGCTTTGGCATCATCTTCACAAATTGATGACTCTTATGAAGGTACTGAAACTGGTGATGCTACAAATTATGGACAGAATGACAGCGGCAATAAGGAAGTTGGGGGATCTGTTGATGAAACTACAACCGATGTTCTTGATGAGCCTGGCGTTGTTCCAACAGCACAGACAGATTCAACAACTGATGAAATCGTTTCTGGTGTGAATGATTTGAAGGTTGTAAATGATATTTCCACTGAGGAACCAAATGTGCAGCATACTCTATCAGCTGAGGACGTGGACAAGCTTCTGGATAAATGTCTTCTTCAAGCCCTGCACATAACAGTGAAAGACAAAGATTTTCCACTTCCTGGAAGCACGCTATGGTCAAATCATGTATTGCCTTGTCGGCCTCCTGGGATGATGCTAGACATCAAGAAATCTTCCTACAAGAAATTGTCTAAGTGGTTGCAAGCTAAATCCTCCGCTGGACTGATTACAGTGAAAGAAGACAAGCATAAAAAAGAGACGGTTTTGTACTCTGTTAACCGTAAGCATCCAGACTATACTTCCTTTAAACCAGAGAAGCAACAGGTAGAGAAAATTGATCAGAGCAATAATCATCACGGTCTGAAAGAAAGTTCATCCAATAAAATGCTTGAAGTAGCAGAGATATACAAGCCAAGTGTGCATGTCAATCCCATTTTTGTTGCTGTGGGGGCAGACACTAGCAAACTTTACACTTCAGCAGAGGCTACTGATATTGTCTTCCAATACGTCGAGAAAGAAAAATTGGAGAAACCAACAGATAAGTCTAGAGTGGTTTTGGATGCAATATTATGCGATTCACTGTTCAAGGGGGCCATTAAGAAAGGAACAACGTATCCTACAGAAATTCACAAAAGAGATATAGGCTCAACATTTATCAGCCGGATGCAACCTCATCACATTGTAACAAGAGGAAGTGAGTCTGTCGTTCGCAAAGGTGCACTAAAAACTATTCAAATAATGACAGAGCGACGCCAAGGAAATAAGAAAGTGACAAGACTTTCTGGGTTGGAAACGTTCTTGTTGGATGCTGAAGCATTGGCTTCAGAGCTTCAGAAAAAGTGTGCTTGCAGTACGACGGTGGCAGAGTTGCCTGGTAAAAAAGGGTATGAAGTTGTGGTCCAAGGTGGAGTCATTGATGACTTGGCAAGACATCTGATCGAACAATATGGAATTCCAAAAAAGTACATTGAAGTACTTGATAAAACAAGGAAATGAGAGGCTAGAGCTGCTATATATCTTACAGGGAACTTACCAATCCCAGTCTTATGATATCAAGTCAAGATCTGCTGTTATCAGCGCCAGCATCATGCAAAGGAAAAAGCCTGAATCAAAAGATATGATAGAAGTTATTGATAATGAGGTATCAAAATTTGATTCATGCAACAAAGTAAAAAGAAGATTCCTCATTCGTCTCTTTAGGCTGGAAAATTGGTTCTAGTTTCATTGACACTGATTGTTTATTTACCTAAAATTTGTTCATCACGTGTTTTCGGCTGCCTTGTGTAAAAGAAAATGTTTGTAGTTAGGACAAACC

Coding sequence (CDS)

ATGTTCAAGAAGGCGTCAGAGGCGAAGTTGCAGCAGAGATTATCTGGTGCAGACAGGAAGAAACTGAAGAGGACTGTTAAAGAAAGGTTCCCTGCAGCTTCCGATGCCGATATTGATCTCTTGCTTCCTCCCAAGGTCGAGTTTTCTGTTGCCAAGTTCCAAAATCGCGTTCACATATATAGCGTTGAAGGAGGCTTTCCAATGTTTTTCGACGTTGATGGGAGAGGAAATGAAATATTCCCCACAGTTTTTGCACTCTGGATGGTACCGGAATTGTTGCCTGCCTTTTTTTTGAAGGGGGGTGAAGTCTCTCGCTTTGTGATTGGAGGAGCAGACTTGATGTTCCCTGGTATTCACGTTCCTGCTGAAGGTCTTCCTTCATTTTTATCTGGGGAACCATGGGCTGTGAAAGTCCCTGGCAATGCATCTCCTATTGCTGTTGGTGTTACCACCATGAGTAGCACCGAAGCACTAAAAGCAGGTTTACGTGGAAAGGCTCTGAGAATTCTACACTACTACAGGGATACCCTTTGGGGATTGGTAGAGGGTCATTATGTGCCCAATGCAGGATTTTTGGAAGATGCTGTATATGGAGATCCTGCTTTGGCATCATCTTCACAAATTGATGACTCTTATGAAGGTACTGAAACTGGTGATGCTACAAATTATGGACAGAATGACAGCGGCAATAAGGAAGTTGGGGGATCTGTTGATGAAACTACAACCGATGTTCTTGATGAGCCTGGCGTTGTTCCAACAGCACAGACAGATTCAACAACTGATGAAATCGTTTCTGGTGTGAATGATTTGAAGGTTGTAAATGATATTTCCACTGAGGAACCAAATGTGCAGCATACTCTATCAGCTGAGGACGTGGACAAGCTTCTGGATAAATGTCTTCTTCAAGCCCTGCACATAACAGTGAAAGACAAAGATTTTCCACTTCCTGGAAGCACGCTATGGTCAAATCATGTATTGCCTTGTCGGCCTCCTGGGATGATGCTAGACATCAAGAAATCTTCCTACAAGAAATTGTCTAAGTGGTTGCAAGCTAAATCCTCCGCTGGACTGATTACAGTGAAAGAAGACAAGCATAAAAAAGAGACGGTTTTGTACTCTGTTAACCGTAAGCATCCAGACTATACTTCCTTTAAACCAGAGAAGCAACAGGTAGAGAAAATTGATCAGAGCAATAATCATCACGGTCTGAAAGAAAGTTCATCCAATAAAATGCTTGAAGTAGCAGAGATATACAAGCCAAGTGTGCATGTCAATCCCATTTTTGTTGCTGTGGGGGCAGACACTAGCAAACTTTACACTTCAGCAGAGGCTACTGATATTGTCTTCCAATACGTCGAGAAAGAAAAATTGGAGAAACCAACAGATAAGTCTAGAGTGGTTTTGGATGCAATATTATGCGATTCACTGTTCAAGGGGGCCATTAAGAAAGGAACAACGTATCCTACAGAAATTCACAAAAGAGATATAGGCTCAACATTTATCAGCCGGATGCAACCTCATCACATTGTAACAAGAGGAAGTGAGTCTGTCGTTCGCAAAGGTGCACTAAAAACTATTCAAATAATGACAGAGCGACGCCAAGGAAATAAGAAAGTGACAAGACTTTCTGGGTTGGAAACGTTCTTGTTGGATGCTGAAGCATTGGCTTCAGAGCTTCAGAAAAAGTGTGCTTGCAGTACGACGGTGGCAGAGTTGCCTGGTAAAAAAGGGTATGAAGTTGTGGTCCAAGGTGGAGTCATTGATGACTTGGCAAGACATCTGATCGAACAATATGGAATTCCAAAAAAGTACATTGAAGTACTTGATAAAACAAGGAAATGA

Protein sequence

MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQNRVHIYSVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHVPAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDTLWGLVEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETGDATNYGQNDSGNKEVGGSVDETTTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLLDKCLLQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITVKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHHGLKESSSNKMLEVAEIYKPSVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAILCDSLFKGAIKKGTTYPTEIHKRDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIMTERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGIPKKYIEVLDKTRK*
Homology
BLAST of CSPI02G24770 vs. ExPASy Swiss-Prot
Match: Q5RA63 (Eukaryotic translation initiation factor 2D OS=Pongo abelii OX=9601 GN=EIF2D PE=2 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 6.5e-71
Identity = 199/624 (31.89%), Postives = 308/624 (49.36%), Query Frame = 0

Query: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQ----NR 60
           MF KA   K    + G+DR+KL+  V   FP      +  L+P K E ++ K      + 
Sbjct: 1   MFAKAFRVKSNTAIKGSDRRKLRADVTTAFPTLGTDQVSELVPGKEELNIVKLYAHKGDA 60

Query: 61  VHIYSVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFP 120
           V +Y V GG P+ F+++     ++PTV+ LW  P+LLP  F     V   ++GGADLM P
Sbjct: 61  VTVY-VSGGNPILFELE---KNLYPTVYTLWSYPDLLPT-FTTWPLVLEKLVGGADLMLP 120

Query: 121 GIHVPAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDT 180
           G+ +P  GLP    G+  A+ + GN +P+A+GV  MS+ E L +GL+G+   +LH Y+D 
Sbjct: 121 GLVMPPAGLPQVQKGDLCAISLVGNRAPVAIGVAAMSTAEMLTSGLKGRGFSVLHTYQDH 180

Query: 181 LWGLVEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETGDATNYGQNDSGNKEVGGS 240
           LW        P+           P    S+ + +  +G+   D+T           + G 
Sbjct: 181 LWRSGNKSSPPSIA---------PLALDSADLSEE-KGSVQMDST-----------LQGD 240

Query: 241 VDETTTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLL 300
           +   T +  +E G V  A+ D +  E     +  + +N  ST+   +Q     E +D+LL
Sbjct: 241 MRHMTLEGEEENGEVHQAREDKSLSEAPEDTS-TRGLNQDSTDSKTLQ-----EQMDELL 300

Query: 301 DKCLLQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAG 360
            +C L AL   VK  D PL  ST   +H+  C P G  LDIKKSSYKKLSK+LQ      
Sbjct: 301 QQCFLHALKCRVKKADLPLLASTFLGSHMFSCCPEGRQLDIKKSSYKKLSKFLQQMQQEQ 360

Query: 361 LITVKEDKHKKETVLYSVNRKHPDYTSF-----KPEKQQVEKIDQSNNHHGLKESSSNKM 420
           +I VKE     E+++ +V+ KHP  TSF      P  Q +++  +   +H          
Sbjct: 361 IIQVKELSKGVESIV-AVDWKHPRITSFVIPEPSPTSQTIQEGSREQPYH---------P 420

Query: 421 LEVAEIYKPSVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAI 480
            ++  +Y     +  +F   G         +E   IV  Y +K  L    +K+ V LD I
Sbjct: 421 PDIKPLYCVPASMTLLFQESGHKKGSFLEGSEVRTIVINYAKKNDLVDADNKNLVRLDPI 480

Query: 481 LCDSLFKGAIKKGTTYPTEIHKRDIGSTFISRMQPHHIVT-RGSESVVRKGALKTIQIMT 540
           LCD + +   K       ++    + +  + ++QP + VT  G E +V+KG +  I I  
Sbjct: 481 LCDCILE---KNEQHTVMKLPWDSLLTRCLEKLQPAYQVTLPGQEPIVKKGRICPIDITL 540

Query: 541 ERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPG-KKGYEVVVQGGVIDD 600
            +R  NKKVT +  LE + LD  ++A+ LQ++C  STTV   PG K   +V +QG  +  
Sbjct: 541 AQRASNKKVTVVRNLEAYGLDPYSVAAILQQRCQASTTVNPAPGAKDSLQVQIQGNQVHH 579

Query: 601 LARHLIEQYGIPKKYIEVLDKTRK 614
           L   L+E+Y +P+K+I+ L+K  K
Sbjct: 601 LGWLLLEEYQLPRKHIQGLEKALK 579

BLAST of CSPI02G24770 vs. ExPASy Swiss-Prot
Match: P41214 (Eukaryotic translation initiation factor 2D OS=Homo sapiens OX=9606 GN=EIF2D PE=1 SV=3)

HSP 1 Score: 269.2 bits (687), Expect = 1.1e-70
Identity = 199/624 (31.89%), Postives = 308/624 (49.36%), Query Frame = 0

Query: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQ----NR 60
           MF KA   K    + G+DR+KL+  V   FP      +  L+P K E ++ K      + 
Sbjct: 1   MFAKAFRVKSNTAIKGSDRRKLRADVTTAFPTLGTDQVSELVPGKEELNIVKLYAHKGDA 60

Query: 61  VHIYSVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFP 120
           V +Y V GG P+ F+++     ++PTV+ LW  P+LLP  F     V   ++GGADLM P
Sbjct: 61  VTVY-VSGGNPILFELE---KNLYPTVYTLWSYPDLLPT-FTTWPLVLEKLVGGADLMLP 120

Query: 121 GIHVPAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDT 180
           G+ +P  GLP    G+  A+ + GN +P+A+GV  MS+ E L +GL+G+   +LH Y+D 
Sbjct: 121 GLVMPPAGLPQVQKGDLCAISLVGNRAPVAIGVAAMSTAEMLTSGLKGRGFSVLHTYQDH 180

Query: 181 LWGLVEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETGDATNYGQNDSGNKEVGGS 240
           LW        P+           P    S+ + +  +G+   D+T           + G 
Sbjct: 181 LWRSGNKSSPPSIA---------PLALDSADLSEE-KGSVQMDST-----------LQGD 240

Query: 241 VDETTTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLL 300
           +   T +  +E G V  A+ D +  E     +  + +N  ST+   +Q     E +D+LL
Sbjct: 241 MRHMTLEGEEENGEVHQAREDKSLSEAPEDTS-TRGLNQDSTDSKTLQ-----EQMDELL 300

Query: 301 DKCLLQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAG 360
            +C L AL   VK  D PL  ST   +H+  C P G  LDIKKSSYKKLSK+LQ      
Sbjct: 301 QQCFLHALKCRVKKADLPLLTSTFLGSHMFSCCPEGRQLDIKKSSYKKLSKFLQQMQQEQ 360

Query: 361 LITVKEDKHKKETVLYSVNRKHPDYTSF-----KPEKQQVEKIDQSNNHHGLKESSSNKM 420
           +I VKE     E+++ +V+ KHP  TSF      P  Q +++  +   +H          
Sbjct: 361 IIQVKELSKGVESIV-AVDWKHPRITSFVIPEPSPTSQTIQEGSREQPYH---------P 420

Query: 421 LEVAEIYKPSVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAI 480
            ++  +Y     +  +F   G         +E   IV  Y +K  L    +K+ V LD I
Sbjct: 421 PDIKPLYCVPASMTLLFQESGHKKGSFLEGSEVRTIVINYAKKNDLVDADNKNLVRLDPI 480

Query: 481 LCDSLFKGAIKKGTTYPTEIHKRDIGSTFISRMQPHHIVT-RGSESVVRKGALKTIQIMT 540
           LCD + +   K       ++    + +  + ++QP + VT  G E +V+KG +  I I  
Sbjct: 481 LCDCILE---KNEQHTVMKLPWDSLLTRCLEKLQPAYQVTLPGQEPIVKKGRICPIDITL 540

Query: 541 ERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPG-KKGYEVVVQGGVIDD 600
            +R  NKKVT +  LE + LD  ++A+ LQ++C  STTV   PG K   +V +QG  +  
Sbjct: 541 AQRASNKKVTVVRNLEAYGLDPYSVAAILQQRCQASTTVNPAPGAKDSLQVQIQGNQVHH 579

Query: 601 LARHLIEQYGIPKKYIEVLDKTRK 614
           L   L+E+Y +P+K+I+ L+K  K
Sbjct: 601 LGWLLLEEYQLPRKHIQGLEKALK 579

BLAST of CSPI02G24770 vs. ExPASy Swiss-Prot
Match: Q61211 (Eukaryotic translation initiation factor 2D OS=Mus musculus OX=10090 GN=Eif2d PE=1 SV=3)

HSP 1 Score: 264.2 bits (674), Expect = 3.6e-69
Identity = 199/620 (32.10%), Postives = 302/620 (48.71%), Query Frame = 0

Query: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQ----NR 60
           MF KA   K    + G+DR+KL+  V   FPA     I  L+P K E +V K      + 
Sbjct: 1   MFAKAFRVKSNTAIKGSDRRKLRADVTAAFPALGTDQISELIPGKEELNVVKLYVHKGDS 60

Query: 61  VHIYSVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFP 120
           V +Y+  GG P+ F+++     ++PTV+ LW  P++LP  F+    V   ++GGADLM P
Sbjct: 61  VTVYT-SGGNPILFELE---KNLYPTVYTLWAYPDILPT-FITWPLVLEKLVGGADLMLP 120

Query: 121 GIHVPAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDT 180
           G+ VP  GLP    G+  A+ + GN +P+A+GV  MS+ + L +GL+GK + +LH Y+D 
Sbjct: 121 GVVVPPTGLPQVQQGDLCAIALVGNRAPVAIGVAAMSTAQMLASGLKGKGVSVLHTYQDH 180

Query: 181 LWGLVEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETGDATNYGQNDSGNKEVGGS 240
           LW   +                 PA+A     D   E    G              + G+
Sbjct: 181 LWRSGDKS-------------SPPAIAPLDPTDSCEEKVHLG--------------LQGN 240

Query: 241 VDETTTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLL 300
           +   T D  +E G VP  +    T          +  +  S +   +Q     E +D LL
Sbjct: 241 LKSLTLDGEEENGQVPLREASEDTSS--------RAPSQDSLDGKPLQ-----EQMDDLL 300

Query: 301 DKCLLQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAG 360
            +C L AL   VK  D PL  STL  +H+  C P G  LDIKKSSYKKLSK+LQ      
Sbjct: 301 LRCFLHALKSRVKKADLPLLTSTLLGSHMFSCCPEGQQLDIKKSSYKKLSKFLQHMQQEQ 360

Query: 361 LITVKEDKHKKETVLYSVNRKHPDYTSFK-PEKQQVEKIDQSNNHHGLKESSSNKMLEVA 420
           ++ VKE     E+++ +V+ +HP  TSF  PE     +  Q      +         ++ 
Sbjct: 361 IVQVKELSKGVESIV-AVDWRHPRITSFVIPEPSLTSQTVQE-----VSREQPYLPPDIK 420

Query: 421 EIYKPSVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAILCDS 480
            +Y    ++  +F+  G         +E   I+  Y ++ +L    +++ V LD ILCD 
Sbjct: 421 SLYCVPANMTQLFLESGHKKGSTLEGSEVRKIITDYAKRNRLVDADNRNLVKLDPILCDC 480

Query: 481 LFKGAIKKGTTYPTEIHKRDIGSTFISRMQPHHIVT-RGSESVVRKGALKTIQIMTERRQ 540
           + +   K      T++    + +  +  MQP + VT  G E +++KG L  I I    + 
Sbjct: 481 ILE---KNEQHLVTKLPWDCLLTRCLKNMQPAYQVTFPGQEPILKKGKLCPIDITLALKT 540

Query: 541 GNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPG-KKGYEVVVQGGVIDDLARH 600
            NKKVT +  LET+ LD  ++A+ LQ++C  ST V+  PG K   +V VQG  I  L + 
Sbjct: 541 YNKKVTVVRNLETYGLDPCSVAAILQQRCQASTIVSPAPGAKDSLQVQVQGNQIHHLGQL 566

Query: 601 LIEQYGIPKKYIEVLDKTRK 614
           L+E+Y +P KYI+ L+K  K
Sbjct: 601 LLEEYRLPGKYIQGLEKAPK 566

BLAST of CSPI02G24770 vs. ExPASy Swiss-Prot
Match: P0CL18 (Eukaryotic translation initiation factor 2D OS=Oryctolagus cuniculus OX=9986 GN=EIF2D PE=1 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 3.6e-69
Identity = 197/620 (31.77%), Postives = 303/620 (48.87%), Query Frame = 0

Query: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQ----NR 60
           MF KA   K    + G+DR+KL+  V   FP      +  L+P K E +  K      + 
Sbjct: 1   MFAKAFRVKSNTAIKGSDRRKLRADVAAAFPTLGTDQVSELVPGKEELNTVKLYAHRGDA 60

Query: 61  VHIYSVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFP 120
           V +Y V GG P+ F+++     ++PTV+ LW  P+LLP  F     V   ++GGADLM P
Sbjct: 61  VTVY-VSGGNPILFELE---KNLYPTVYMLWSHPDLLPT-FTTWPLVLEKLVGGADLMLP 120

Query: 121 GIHVPAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDT 180
           G+ VP  GLP    G+  A+ + GN +P+A+GV  MS+ E L +GL+G+   +LH Y+D 
Sbjct: 121 GLVVPPAGLPQVQKGDLCAIALVGNRAPVAIGVAAMSTEEMLTSGLKGRGFSVLHTYQDH 180

Query: 181 LWGLVEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETGDATNYGQNDSGNKEVGGS 240
           LW   +    P+           P +   + ++D  E  +             N  + G 
Sbjct: 181 LWRSGDKSSPPSIA---------PLVVDPADLNDDKESVQV------------NPVLQG- 240

Query: 241 VDETTTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLL 300
                    +E G   TA +++T+    +G  D         ++P    TL  E +D+LL
Sbjct: 241 ---------EEEGNGETALSEATS----AGGQD---------QDPTDSRTLQ-EQMDELL 300

Query: 301 DKCLLQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAG 360
            +C L AL   ++  D PL  STL  +H+  C P G  LDIKKSSYKKLSK+LQ      
Sbjct: 301 QQCFLHALKCRIQKADLPLLTSTLLGSHMFSCCPEGHQLDIKKSSYKKLSKFLQHMQQEQ 360

Query: 361 LITVKEDKHKKETVLYSVNRKHPDYTSFK-PEKQQVEKIDQSNNHHGLKESSSNKMLEVA 420
           ++ VKE     E+V+ +V+ +HP  TSF  PE     +  Q  +          +  ++ 
Sbjct: 361 IVQVKELSRGVESVV-AVDWRHPRITSFVIPEPSLTSQSVQEGS-----REQPYRPPDIK 420

Query: 421 EIYKPSVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAILCDS 480
            +Y     +  +F   G     +   +E   I+  Y +K  L    +K+ V LD +LCD 
Sbjct: 421 PLYCVPASMTLLFQESGHKKGSVLEGSEVRAIIINYAKKNDLVDADNKNLVKLDPVLCDC 480

Query: 481 LFKGAIKKGTTYPTEIHKRDIGSTFISRMQPHHIVT-RGSESVVRKGALKTIQIMTERRQ 540
           L +   K       ++    + +  + ++QP + VT  G E VV+KG +  I I   +R 
Sbjct: 481 LLE---KHEQHSIMKLPWDSLVTRCLEKLQPAYQVTFPGQEPVVKKGKICPIDITLAQRA 540

Query: 541 GNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPG-KKGYEVVVQGGVIDDLARH 600
            NKKVT +  LE + LD  ++A+ LQ++C  STTV   PG K   +V +QG  +  L R 
Sbjct: 541 SNKKVTVVRNLEAYGLDPGSVAATLQQRCQASTTVTPAPGAKDSLQVQIQGNQVHHLGRL 561

Query: 601 LIEQYGIPKKYIEVLDKTRK 614
           L+E+Y +P+KYI+ L+   K
Sbjct: 601 LLEEYHLPRKYIQGLEHAPK 561

BLAST of CSPI02G24770 vs. ExPASy Swiss-Prot
Match: Q5PPG7 (Eukaryotic translation initiation factor 2D OS=Rattus norvegicus OX=10116 GN=Eif2d PE=1 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 4.7e-69
Identity = 197/623 (31.62%), Postives = 304/623 (48.80%), Query Frame = 0

Query: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQ----NR 60
           MF KA   K    + G+DR+KL+  V   FP      +  L+P K E +V K      + 
Sbjct: 1   MFAKAFRVKSNTAIKGSDRRKLRADVTVAFPTLGTDQVSELIPGKEELNVVKLYAHKGDA 60

Query: 61  VHIYSVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFP 120
           V +Y+  GG P+ F+++     ++PTV+ LW  P+LLPA F+    V   ++GGADLM P
Sbjct: 61  VTVYT-SGGNPILFELE---KNLYPTVYTLWSHPDLLPA-FITWPLVLEKLVGGADLMLP 120

Query: 121 GIHVPAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDT 180
           G+ VP  GLP    G+  A+ + GN +P+AVGV  MS+ + L +GL+GK + +LH Y+D 
Sbjct: 121 GVVVPPTGLPQVQQGDLCAIALVGNRAPVAVGVAAMSTAQMLASGLKGKGISVLHTYQDH 180

Query: 181 LWGLVEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETGDATNYGQNDSGNKEVGGS 240
           LW   +                 PA+A     D   E  + G              + G+
Sbjct: 181 LWRSGDKS-------------SPPAIAPLDPTDSCEEKADLG--------------LHGN 240

Query: 241 VDETTTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLL 300
           +   + +  +E G VP  +                  N  +  + +V      E +D+LL
Sbjct: 241 LRSLSLEGEEENGQVPNPEASDDP-------------NSRALSQDSVDGKPLQEQMDELL 300

Query: 301 DKCLLQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAG 360
           ++C L AL   VK  D PL  STL  +H+  C P G  LDIKKSSYKKLSK+LQ      
Sbjct: 301 EQCFLHALKSRVKKADLPLLTSTLLGSHMFSCCPEGQQLDIKKSSYKKLSKFLQHMQQEQ 360

Query: 361 LITVKEDKHKKETVLYSVNRKHPDYTSF-KPEKQQVEKIDQSNNHHGLKESSSNKML--- 420
           ++ VKE     E+++ +V+ +HP  TSF  PE     +         ++E S  K     
Sbjct: 361 IVQVKELSKGVESIV-AVDWRHPRITSFIVPEPSLASQT--------VQEGSREKPYLPP 420

Query: 421 EVAEIYKPSVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAIL 480
           ++  +Y    ++  +F+  G         +E   I+  Y ++  L    +++ V LD IL
Sbjct: 421 DIKSLYCVPANMTQLFLESGHKKGSTLEGSEVRRIITDYAKRNNLVDADNRNLVKLDPIL 480

Query: 481 CDSLFKGAIKKGTTYPTEIHKRDIGSTFISRMQPHHIVT-RGSESVVRKGALKTIQIMTE 540
           CD + +   K       ++    + +  +  +QP + VT  G E +++KG L  I I   
Sbjct: 481 CDCILE---KNEQHLVMKLPWDSLLTRCLKNLQPAYQVTFPGQEPIIKKGKLCPIDITLV 540

Query: 541 RRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPG-KKGYEVVVQGGVIDDL 600
            +  NKKVT +  LET+ LD  ++A+ LQ++C  ST V+  PG K   +V VQG  I  L
Sbjct: 541 LKTYNKKVTVVRNLETYGLDPCSVAAILQQRCQASTIVSPAPGAKDSLQVQVQGNQIHHL 566

Query: 601 ARHLIEQYGIPKKYIEVLDKTRK 614
            + L+E+Y +P KYI+ L+K  K
Sbjct: 601 GQLLLEEYRLPGKYIQGLEKAPK 566

BLAST of CSPI02G24770 vs. ExPASy TrEMBL
Match: A0A0A0LQM2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G401420 PE=3 SV=1)

HSP 1 Score: 1200.3 bits (3104), Expect = 0.0e+00
Identity = 611/613 (99.67%), Postives = 611/613 (99.67%), Query Frame = 0

Query: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQNRVHIY 60
           MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQNRVHIY
Sbjct: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQNRVHIY 60

Query: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHV 120
           SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHV
Sbjct: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHV 120

Query: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDTLWGL 180
           PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDTLWG 
Sbjct: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDTLWGS 180

Query: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETGDATNYGQNDSGNKEVGGSVDET 240
           VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYE TETGDATNYGQNDSGNKEVGGSVDET
Sbjct: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEDTETGDATNYGQNDSGNKEVGGSVDET 240

Query: 241 TTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLLDKCL 300
           TTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLLDKCL
Sbjct: 241 TTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLLDKCL 300

Query: 301 LQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV 360
           LQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV
Sbjct: 301 LQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV 360

Query: 361 KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHHGLKESSSNKMLEVAEIYKP 420
           KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHHGLKESSSNKMLEVAEIYKP
Sbjct: 361 KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHHGLKESSSNKMLEVAEIYKP 420

Query: 421 SVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAILCDSLFKGA 480
           SVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAILCDSLFKGA
Sbjct: 421 SVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAILCDSLFKGA 480

Query: 481 IKKGTTYPTEIHKRDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIMTERRQGNKKVT 540
           IKKGTTYPTEIHKRDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIMTERRQGNKKVT
Sbjct: 481 IKKGTTYPTEIHKRDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIMTERRQGNKKVT 540

Query: 541 RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI 600
           RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI
Sbjct: 541 RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI 600

Query: 601 PKKYIEVLDKTRK 614
           PKKYIEVLDKTRK
Sbjct: 601 PKKYIEVLDKTRK 613

BLAST of CSPI02G24770 vs. ExPASy TrEMBL
Match: A0A1S3B3L6 (eukaryotic translation initiation factor 2D isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485399 PE=3 SV=1)

HSP 1 Score: 1166.8 bits (3017), Expect = 0.0e+00
Identity = 595/613 (97.06%), Postives = 603/613 (98.37%), Query Frame = 0

Query: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQNRVHIY 60
           MFKKA+EAKLQQRLSGADRKKLKRTVKERFP  SDADIDLLLPPKVEFSVAKFQNRVHIY
Sbjct: 1   MFKKAAEAKLQQRLSGADRKKLKRTVKERFPGPSDADIDLLLPPKVEFSVAKFQNRVHIY 60

Query: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHV 120
           SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIH+
Sbjct: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHI 120

Query: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDTLWGL 180
           PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRD+LWG 
Sbjct: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGS 180

Query: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETGDATNYGQNDSGNKEVGGSVDET 240
           VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTET DA+NYGQNDSG+KEVGGSVDET
Sbjct: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETDDASNYGQNDSGDKEVGGSVDET 240

Query: 241 TTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLLDKCL 300
           TTDVLDEPGVV TAQTDST DEIVSGVNDLKVVNDISTEEP VQHTLSAEDVDKLLDKCL
Sbjct: 241 TTDVLDEPGVVLTAQTDSTIDEIVSGVNDLKVVNDISTEEPKVQHTLSAEDVDKLLDKCL 300

Query: 301 LQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV 360
           LQALH TVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV
Sbjct: 301 LQALHTTVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV 360

Query: 361 KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHHGLKESSSNKMLEVAEIYKP 420
           KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNN HG KESSSNKMLEVAEIYKP
Sbjct: 361 KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNN-HGPKESSSNKMLEVAEIYKP 420

Query: 421 SVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAILCDSLFKGA 480
           SVHVNPIFVAVGADTSKLYTSAEATDIVF+YVEKEKLEKPTDKSRVVLDAILCD+LFKGA
Sbjct: 421 SVHVNPIFVAVGADTSKLYTSAEATDIVFEYVEKEKLEKPTDKSRVVLDAILCDALFKGA 480

Query: 481 IKKGTTYPTEIHKRDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIMTERRQGNKKVT 540
           IKKGTTYPTEIHKRDIGSTFISRMQPHH+VTRGSESVVRKGALKTIQIMTERRQGNKKVT
Sbjct: 481 IKKGTTYPTEIHKRDIGSTFISRMQPHHVVTRGSESVVRKGALKTIQIMTERRQGNKKVT 540

Query: 541 RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI 600
           RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI
Sbjct: 541 RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI 600

Query: 601 PKKYIEVLDKTRK 614
           PKKYIEVLDKTRK
Sbjct: 601 PKKYIEVLDKTRK 612

BLAST of CSPI02G24770 vs. ExPASy TrEMBL
Match: A0A5D3C5R8 (Eukaryotic translation initiation factor 2D isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G00810 PE=3 SV=1)

HSP 1 Score: 1153.7 bits (2983), Expect = 0.0e+00
Identity = 588/607 (96.87%), Postives = 597/607 (98.35%), Query Frame = 0

Query: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQNRVHIY 60
           MFKKA+EAKLQQRLSGADRKKLKRTVKERFP  SDADIDLLLPPKVEFSVAKFQNRVHIY
Sbjct: 1   MFKKAAEAKLQQRLSGADRKKLKRTVKERFPGPSDADIDLLLPPKVEFSVAKFQNRVHIY 60

Query: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHV 120
           SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIH+
Sbjct: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHI 120

Query: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDTLWGL 180
           PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRD+LWG 
Sbjct: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGS 180

Query: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETGDATNYGQNDSGNKEVGGSVDET 240
           VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTET DA+NYGQNDSG+KEVGGSVDET
Sbjct: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETDDASNYGQNDSGDKEVGGSVDET 240

Query: 241 TTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLLDKCL 300
           TTDVLDEPGVV TAQTDST DEIVSGVNDLKVVNDISTEEP VQHTLSAEDVDKLLDKCL
Sbjct: 241 TTDVLDEPGVVLTAQTDSTIDEIVSGVNDLKVVNDISTEEPKVQHTLSAEDVDKLLDKCL 300

Query: 301 LQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV 360
           LQALH TVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV
Sbjct: 301 LQALHTTVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV 360

Query: 361 KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHHGLKESSSNKMLEVAEIYKP 420
           KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNN HG KESSSNKMLEVAEIYKP
Sbjct: 361 KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNN-HGPKESSSNKMLEVAEIYKP 420

Query: 421 SVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAILCDSLFKGA 480
           SVHVNPIFVAVGADTSKLYTSAEATDIVF+YVEKEKLEKPTDKSRVVLDAILCD+LFKGA
Sbjct: 421 SVHVNPIFVAVGADTSKLYTSAEATDIVFEYVEKEKLEKPTDKSRVVLDAILCDALFKGA 480

Query: 481 IKKGTTYPTEIHKRDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIMTERRQGNKKVT 540
           IKKGTTYPTEIHKRDIGSTFISRMQPHH+VTRGSESVVRKGALKTIQIMTERRQGNKKVT
Sbjct: 481 IKKGTTYPTEIHKRDIGSTFISRMQPHHVVTRGSESVVRKGALKTIQIMTERRQGNKKVT 540

Query: 541 RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI 600
           RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI
Sbjct: 541 RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI 600

Query: 601 PKKYIEV 608
           PKKYIE+
Sbjct: 601 PKKYIEL 606

BLAST of CSPI02G24770 vs. ExPASy TrEMBL
Match: A0A6J1DF37 (eukaryotic translation initiation factor 2D OS=Momordica charantia OX=3673 GN=LOC111020231 PE=3 SV=1)

HSP 1 Score: 1067.4 bits (2759), Expect = 2.3e-308
Identity = 547/613 (89.23%), Postives = 571/613 (93.15%), Query Frame = 0

Query: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQNRVHIY 60
           MFKKA+EAKL QRLSGADRKKLKRTVKERF  ASDADID+LLPPKVE +V+KFQNRV IY
Sbjct: 1   MFKKAAEAKLHQRLSGADRKKLKRTVKERFAGASDADIDVLLPPKVELTVSKFQNRVQIY 60

Query: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHV 120
           SVEGGFPMFFDVDGRG EIFPTVFALWMVPELLPAF LKGGEVSRFV+GGADLMFPGIHV
Sbjct: 61  SVEGGFPMFFDVDGRGAEIFPTVFALWMVPELLPAFVLKGGEVSRFVLGGADLMFPGIHV 120

Query: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDTLWGL 180
           P EGLPSFLSGEPWAV VPGNA+PIAVGVTTMSS EA KAGLRGKALRILHYYRD+LW  
Sbjct: 121 PTEGLPSFLSGEPWAVNVPGNAAPIAVGVTTMSSAEAFKAGLRGKALRILHYYRDSLWES 180

Query: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETGDATNYGQNDSGNKEVGGSVDET 240
           VEGHYVPNAGFLED VYGDP LASSSQ  DS EG +   A+NY QND  NKE G S DE 
Sbjct: 181 VEGHYVPNAGFLEDVVYGDPLLASSSQTGDSCEGID--GASNYKQNDIDNKE-GESTDEP 240

Query: 241 TTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLLDKCL 300
           T+DVL EPG V TAQTDSTTDEI+SG+NDLKVVND+ST EPNVQHTLSAEDVD LLDKCL
Sbjct: 241 TSDVLAEPGTVLTAQTDSTTDEIISGLNDLKVVNDVSTGEPNVQHTLSAEDVDTLLDKCL 300

Query: 301 LQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV 360
           LQALH TVKDKDFPLPGSTLWSNHVLPCRPPGM LDIKKSSYKKLSKWLQAKSS GLITV
Sbjct: 301 LQALHTTVKDKDFPLPGSTLWSNHVLPCRPPGMTLDIKKSSYKKLSKWLQAKSSTGLITV 360

Query: 361 KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHHGLKESSSNKMLEVAEIYKP 420
           KEDKHKKETVLYS NRKHPDYTSFKPEKQQVEKIDQS N HG +ESSSNKMLEVAEIYKP
Sbjct: 361 KEDKHKKETVLYSANRKHPDYTSFKPEKQQVEKIDQSGN-HGPRESSSNKMLEVAEIYKP 420

Query: 421 SVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAILCDSLFKGA 480
           SVHVNPIF AVGADTSKLYT+AEATDIVF+YVEKEKL+KPT+KSR+VLDAILCD+LFKG+
Sbjct: 421 SVHVNPIFAAVGADTSKLYTAAEATDIVFKYVEKEKLDKPTEKSRIVLDAILCDALFKGS 480

Query: 481 IKKGTTYPTEIHKRDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIMTERRQGNKKVT 540
           IKKGTTYPTEIHK+DIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIMTERRQGNKKVT
Sbjct: 481 IKKGTTYPTEIHKKDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIMTERRQGNKKVT 540

Query: 541 RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI 600
           RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEV+VQGGVIDDLARHL+EQYGI
Sbjct: 541 RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVLVQGGVIDDLARHLVEQYGI 600

Query: 601 PKKYIEVLDKTRK 614
           PKKYIEVLDKTRK
Sbjct: 601 PKKYIEVLDKTRK 609

BLAST of CSPI02G24770 vs. ExPASy TrEMBL
Match: A0A6J1FGK8 (eukaryotic translation initiation factor 2D OS=Cucurbita moschata OX=3662 GN=LOC111445306 PE=3 SV=1)

HSP 1 Score: 1066.6 bits (2757), Expect = 3.8e-308
Identity = 547/613 (89.23%), Postives = 573/613 (93.47%), Query Frame = 0

Query: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQNRVHIY 60
           MFKKA+EAKL QRLSGADRKKLKRTVK RFP ASDADID+LLPPKVE SVAKFQNRVHIY
Sbjct: 1   MFKKAAEAKLHQRLSGADRKKLKRTVKGRFPGASDADIDVLLPPKVELSVAKFQNRVHIY 60

Query: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHV 120
           S+EGGFPMFFDVDGRG E+FPTVFALWMVPELLPAF LKGGEVSRFVIGGADLMFPGIHV
Sbjct: 61  SLEGGFPMFFDVDGRGTEVFPTVFALWMVPELLPAFVLKGGEVSRFVIGGADLMFPGIHV 120

Query: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDTLWGL 180
           PAEGLPSFLSGEPWAVKVPGN SPIAVGVTTMSSTEALKAGLRGKALRILHYYRD+LW  
Sbjct: 121 PAEGLPSFLSGEPWAVKVPGNPSPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWES 180

Query: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETGDATNYGQNDSGNKEVGGSVDET 240
           VEGHYVPNAGFLED VYGDPALASSSQ D+S EGT+  +A+N  QND  NKEVGGSV+ET
Sbjct: 181 VEGHYVPNAGFLEDVVYGDPALASSSQTDNSCEGTD--EASNNEQNDINNKEVGGSVNET 240

Query: 241 TTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLLDKCL 300
           T+DVLDE G   TAQ DS+TD+IVSG+NDL VVNDI  EE NVQHTLS EDVD LLDKCL
Sbjct: 241 TSDVLDELGTPLTAQADSSTDKIVSGMNDLNVVNDIPPEESNVQHTLSTEDVDMLLDKCL 300

Query: 301 LQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV 360
           LQALH TVKDKDFPLPGSTLWSNHVLPCRPPGM LDIKKSSYKKLSKWLQAKSS GLITV
Sbjct: 301 LQALHTTVKDKDFPLPGSTLWSNHVLPCRPPGMTLDIKKSSYKKLSKWLQAKSSTGLITV 360

Query: 361 KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHHGLKESSSNKMLEVAEIYKP 420
           KEDKHKKETVLYSVNRKHPDYTSFKPEK QVEKIDQ +N H  KESSSNKMLEV EIYKP
Sbjct: 361 KEDKHKKETVLYSVNRKHPDYTSFKPEKPQVEKIDQIDN-HAPKESSSNKMLEVTEIYKP 420

Query: 421 SVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAILCDSLFKGA 480
           SVHVNPIFVAVGADTSKLYT+AEA+DIVF+YVEKEKL+K T+K +VVLDAILCD+LFKGA
Sbjct: 421 SVHVNPIFVAVGADTSKLYTAAEASDIVFKYVEKEKLDKLTEKFKVVLDAILCDALFKGA 480

Query: 481 IKKGTTYPTEIHKRDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIMTERRQGNKKVT 540
           IKKGTTYPTEIHK+DIGSTFISRMQPHH+VTRGSESVVRKGALKTIQIMTERRQGNKKVT
Sbjct: 481 IKKGTTYPTEIHKKDIGSTFISRMQPHHVVTRGSESVVRKGALKTIQIMTERRQGNKKVT 540

Query: 541 RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI 600
           RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEV+VQGGVIDDLARHL+EQYGI
Sbjct: 541 RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVLVQGGVIDDLARHLVEQYGI 600

Query: 601 PKKYIEVLDKTRK 614
           PKKYIEVLDKTRK
Sbjct: 601 PKKYIEVLDKTRK 610

BLAST of CSPI02G24770 vs. NCBI nr
Match: XP_011649889.1 (eukaryotic translation initiation factor 2D [Cucumis sativus] >XP_031737209.1 eukaryotic translation initiation factor 2D [Cucumis sativus] >KGN63082.1 hypothetical protein Csa_022079 [Cucumis sativus])

HSP 1 Score: 1200.3 bits (3104), Expect = 0.0e+00
Identity = 611/613 (99.67%), Postives = 611/613 (99.67%), Query Frame = 0

Query: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQNRVHIY 60
           MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQNRVHIY
Sbjct: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQNRVHIY 60

Query: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHV 120
           SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHV
Sbjct: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHV 120

Query: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDTLWGL 180
           PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDTLWG 
Sbjct: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDTLWGS 180

Query: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETGDATNYGQNDSGNKEVGGSVDET 240
           VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYE TETGDATNYGQNDSGNKEVGGSVDET
Sbjct: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEDTETGDATNYGQNDSGNKEVGGSVDET 240

Query: 241 TTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLLDKCL 300
           TTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLLDKCL
Sbjct: 241 TTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLLDKCL 300

Query: 301 LQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV 360
           LQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV
Sbjct: 301 LQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV 360

Query: 361 KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHHGLKESSSNKMLEVAEIYKP 420
           KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHHGLKESSSNKMLEVAEIYKP
Sbjct: 361 KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHHGLKESSSNKMLEVAEIYKP 420

Query: 421 SVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAILCDSLFKGA 480
           SVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAILCDSLFKGA
Sbjct: 421 SVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAILCDSLFKGA 480

Query: 481 IKKGTTYPTEIHKRDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIMTERRQGNKKVT 540
           IKKGTTYPTEIHKRDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIMTERRQGNKKVT
Sbjct: 481 IKKGTTYPTEIHKRDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIMTERRQGNKKVT 540

Query: 541 RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI 600
           RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI
Sbjct: 541 RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI 600

Query: 601 PKKYIEVLDKTRK 614
           PKKYIEVLDKTRK
Sbjct: 601 PKKYIEVLDKTRK 613

BLAST of CSPI02G24770 vs. NCBI nr
Match: XP_008441190.1 (PREDICTED: eukaryotic translation initiation factor 2D isoform X1 [Cucumis melo])

HSP 1 Score: 1166.8 bits (3017), Expect = 0.0e+00
Identity = 595/613 (97.06%), Postives = 603/613 (98.37%), Query Frame = 0

Query: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQNRVHIY 60
           MFKKA+EAKLQQRLSGADRKKLKRTVKERFP  SDADIDLLLPPKVEFSVAKFQNRVHIY
Sbjct: 1   MFKKAAEAKLQQRLSGADRKKLKRTVKERFPGPSDADIDLLLPPKVEFSVAKFQNRVHIY 60

Query: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHV 120
           SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIH+
Sbjct: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHI 120

Query: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDTLWGL 180
           PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRD+LWG 
Sbjct: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGS 180

Query: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETGDATNYGQNDSGNKEVGGSVDET 240
           VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTET DA+NYGQNDSG+KEVGGSVDET
Sbjct: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETDDASNYGQNDSGDKEVGGSVDET 240

Query: 241 TTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLLDKCL 300
           TTDVLDEPGVV TAQTDST DEIVSGVNDLKVVNDISTEEP VQHTLSAEDVDKLLDKCL
Sbjct: 241 TTDVLDEPGVVLTAQTDSTIDEIVSGVNDLKVVNDISTEEPKVQHTLSAEDVDKLLDKCL 300

Query: 301 LQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV 360
           LQALH TVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV
Sbjct: 301 LQALHTTVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV 360

Query: 361 KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHHGLKESSSNKMLEVAEIYKP 420
           KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNN HG KESSSNKMLEVAEIYKP
Sbjct: 361 KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNN-HGPKESSSNKMLEVAEIYKP 420

Query: 421 SVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAILCDSLFKGA 480
           SVHVNPIFVAVGADTSKLYTSAEATDIVF+YVEKEKLEKPTDKSRVVLDAILCD+LFKGA
Sbjct: 421 SVHVNPIFVAVGADTSKLYTSAEATDIVFEYVEKEKLEKPTDKSRVVLDAILCDALFKGA 480

Query: 481 IKKGTTYPTEIHKRDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIMTERRQGNKKVT 540
           IKKGTTYPTEIHKRDIGSTFISRMQPHH+VTRGSESVVRKGALKTIQIMTERRQGNKKVT
Sbjct: 481 IKKGTTYPTEIHKRDIGSTFISRMQPHHVVTRGSESVVRKGALKTIQIMTERRQGNKKVT 540

Query: 541 RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI 600
           RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI
Sbjct: 541 RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI 600

Query: 601 PKKYIEVLDKTRK 614
           PKKYIEVLDKTRK
Sbjct: 601 PKKYIEVLDKTRK 612

BLAST of CSPI02G24770 vs. NCBI nr
Match: KAA0037038.1 (eukaryotic translation initiation factor 2D isoform X1 [Cucumis melo var. makuwa] >TYK06612.1 eukaryotic translation initiation factor 2D isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1153.7 bits (2983), Expect = 0.0e+00
Identity = 588/607 (96.87%), Postives = 597/607 (98.35%), Query Frame = 0

Query: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQNRVHIY 60
           MFKKA+EAKLQQRLSGADRKKLKRTVKERFP  SDADIDLLLPPKVEFSVAKFQNRVHIY
Sbjct: 1   MFKKAAEAKLQQRLSGADRKKLKRTVKERFPGPSDADIDLLLPPKVEFSVAKFQNRVHIY 60

Query: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHV 120
           SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIH+
Sbjct: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHI 120

Query: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDTLWGL 180
           PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRD+LWG 
Sbjct: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGS 180

Query: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETGDATNYGQNDSGNKEVGGSVDET 240
           VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTET DA+NYGQNDSG+KEVGGSVDET
Sbjct: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETDDASNYGQNDSGDKEVGGSVDET 240

Query: 241 TTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLLDKCL 300
           TTDVLDEPGVV TAQTDST DEIVSGVNDLKVVNDISTEEP VQHTLSAEDVDKLLDKCL
Sbjct: 241 TTDVLDEPGVVLTAQTDSTIDEIVSGVNDLKVVNDISTEEPKVQHTLSAEDVDKLLDKCL 300

Query: 301 LQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV 360
           LQALH TVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV
Sbjct: 301 LQALHTTVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV 360

Query: 361 KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHHGLKESSSNKMLEVAEIYKP 420
           KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNN HG KESSSNKMLEVAEIYKP
Sbjct: 361 KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNN-HGPKESSSNKMLEVAEIYKP 420

Query: 421 SVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAILCDSLFKGA 480
           SVHVNPIFVAVGADTSKLYTSAEATDIVF+YVEKEKLEKPTDKSRVVLDAILCD+LFKGA
Sbjct: 421 SVHVNPIFVAVGADTSKLYTSAEATDIVFEYVEKEKLEKPTDKSRVVLDAILCDALFKGA 480

Query: 481 IKKGTTYPTEIHKRDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIMTERRQGNKKVT 540
           IKKGTTYPTEIHKRDIGSTFISRMQPHH+VTRGSESVVRKGALKTIQIMTERRQGNKKVT
Sbjct: 481 IKKGTTYPTEIHKRDIGSTFISRMQPHHVVTRGSESVVRKGALKTIQIMTERRQGNKKVT 540

Query: 541 RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI 600
           RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI
Sbjct: 541 RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI 600

Query: 601 PKKYIEV 608
           PKKYIE+
Sbjct: 601 PKKYIEL 606

BLAST of CSPI02G24770 vs. NCBI nr
Match: XP_038886638.1 (eukaryotic translation initiation factor 2D [Benincasa hispida] >XP_038886639.1 eukaryotic translation initiation factor 2D [Benincasa hispida])

HSP 1 Score: 1130.2 bits (2922), Expect = 0.0e+00
Identity = 577/613 (94.13%), Postives = 591/613 (96.41%), Query Frame = 0

Query: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQNRVHIY 60
           MFKKA+EAKL QRLSGADRKKLKRTVKERFP ASDADID+LLPPKVEFSVAKFQNRVHIY
Sbjct: 1   MFKKAAEAKLHQRLSGADRKKLKRTVKERFPGASDADIDVLLPPKVEFSVAKFQNRVHIY 60

Query: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHV 120
           S+EGGFPMFFDVDGRG +IFPTVFALWMVPELLPAF LKGGEVSR+VIGGADLMFPGIHV
Sbjct: 61  SLEGGFPMFFDVDGRGTDIFPTVFALWMVPELLPAFILKGGEVSRYVIGGADLMFPGIHV 120

Query: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDTLWGL 180
           PAEGLPSF SGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRD+LWG 
Sbjct: 121 PAEGLPSFSSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGS 180

Query: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETGDATNYGQNDSGNKEVGGSVDET 240
           VEGHYVPNAGFLEDAVYGDPALASSSQ D S EGTE  DA+NYGQNDSGNKEVGGSVDET
Sbjct: 181 VEGHYVPNAGFLEDAVYGDPALASSSQTDGSCEGTEADDASNYGQNDSGNKEVGGSVDET 240

Query: 241 TTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLLDKCL 300
           T+DVLDE GVV TAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLLDKCL
Sbjct: 241 TSDVLDESGVVLTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLLDKCL 300

Query: 301 LQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV 360
           LQALH TVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSS GLITV
Sbjct: 301 LQALHTTVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSTGLITV 360

Query: 361 KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHHGLKESSSNKMLEVAEIYKP 420
           +EDKHKKETVLYSVN KHPDY SFKPEKQQVEKIDQSNN HG KESSSNKMLEVAEIYKP
Sbjct: 361 REDKHKKETVLYSVNHKHPDYISFKPEKQQVEKIDQSNN-HGPKESSSNKMLEVAEIYKP 420

Query: 421 SVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAILCDSLFKGA 480
           SVHVNPIFV VGADTSKLYT+AEATDIVFQYVEKEKLEKPTDK+RVVLDAILCD+LFKGA
Sbjct: 421 SVHVNPIFVTVGADTSKLYTAAEATDIVFQYVEKEKLEKPTDKTRVVLDAILCDALFKGA 480

Query: 481 IKKGTTYPTEIHKRDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIMTERRQGNKKVT 540
           IKKGTTYPTEIHK+DIGSTFISRMQPHHIVTRGSESVV KGALKTIQIMTERRQGNKKVT
Sbjct: 481 IKKGTTYPTEIHKKDIGSTFISRMQPHHIVTRGSESVVCKGALKTIQIMTERRQGNKKVT 540

Query: 541 RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI 600
           RLSGL+TFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVID LARHLIEQYGI
Sbjct: 541 RLSGLQTFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDGLARHLIEQYGI 600

Query: 601 PKKYIEVLDKTRK 614
           PKKYIEVLDKTRK
Sbjct: 601 PKKYIEVLDKTRK 612

BLAST of CSPI02G24770 vs. NCBI nr
Match: XP_023550339.1 (eukaryotic translation initiation factor 2D [Cucurbita pepo subsp. pepo] >XP_023550340.1 eukaryotic translation initiation factor 2D [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1068.5 bits (2762), Expect = 2.1e-308
Identity = 547/613 (89.23%), Postives = 574/613 (93.64%), Query Frame = 0

Query: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQNRVHIY 60
           MFKKA+EAKL QRLSGADRKKLKRTVK RFP ASDADID+LLPPKVE SVAKFQNRVHIY
Sbjct: 1   MFKKAAEAKLHQRLSGADRKKLKRTVKGRFPGASDADIDVLLPPKVELSVAKFQNRVHIY 60

Query: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHV 120
           S+EGGFPMFFDVDGRG E+FPTVFALWMVPELLPAF LKGGEVSRFVIGGADLMFPGIHV
Sbjct: 61  SLEGGFPMFFDVDGRGTEVFPTVFALWMVPELLPAFVLKGGEVSRFVIGGADLMFPGIHV 120

Query: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDTLWGL 180
           PAEGLPSFLSGEPWAVKVPGN SPIAVGVTTMSSTEALKAGLRGKALRILHYYRD+LW  
Sbjct: 121 PAEGLPSFLSGEPWAVKVPGNPSPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWES 180

Query: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETGDATNYGQNDSGNKEVGGSVDET 240
           VEGHYVPNAGFLED VYGDPALASSSQ D+S EGT+  +A+N  QND  NKEVGGSV+ET
Sbjct: 181 VEGHYVPNAGFLEDVVYGDPALASSSQTDNSCEGTD--EASNNEQNDINNKEVGGSVNET 240

Query: 241 TTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLLDKCL 300
           T+DVLDE G   TAQ DS+TD+IVSG++DL VVNDI  EE NVQHTLS EDVD LLDKCL
Sbjct: 241 TSDVLDELGTPLTAQADSSTDKIVSGMSDLNVVNDIPPEESNVQHTLSTEDVDMLLDKCL 300

Query: 301 LQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLITV 360
           LQALH TVKDKDFPLPGSTLWSNHVLPCRPPGM LDIKKSSYKKLSKWLQAKSS GLITV
Sbjct: 301 LQALHTTVKDKDFPLPGSTLWSNHVLPCRPPGMTLDIKKSSYKKLSKWLQAKSSTGLITV 360

Query: 361 KEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHHGLKESSSNKMLEVAEIYKP 420
           KEDKHKKETVLYSVNRKHPDYTSFKPEK QVEKIDQ +N H  KESSSNKMLEV EIYKP
Sbjct: 361 KEDKHKKETVLYSVNRKHPDYTSFKPEKPQVEKIDQIDN-HAPKESSSNKMLEVTEIYKP 420

Query: 421 SVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAILCDSLFKGA 480
           SVHVNPIFVAVGADTSKLYT+AEA+DIVF+YVEKEKL+KPT+K +VVLDAILCD+LFKGA
Sbjct: 421 SVHVNPIFVAVGADTSKLYTAAEASDIVFKYVEKEKLDKPTEKFKVVLDAILCDALFKGA 480

Query: 481 IKKGTTYPTEIHKRDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIMTERRQGNKKVT 540
           IKKGTTYPTEIHK+DIGSTFISRMQPHH+VTRGSESVVRKGALKTIQIMTERRQGNKKVT
Sbjct: 481 IKKGTTYPTEIHKKDIGSTFISRMQPHHVVTRGSESVVRKGALKTIQIMTERRQGNKKVT 540

Query: 541 RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQYGI 600
           RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEV+VQGGVIDDLARHL+EQYGI
Sbjct: 541 RLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVLVQGGVIDDLARHLVEQYGI 600

Query: 601 PKKYIEVLDKTRK 614
           PKKYIEVLDKTRK
Sbjct: 601 PKKYIEVLDKTRK 610

BLAST of CSPI02G24770 vs. TAIR 10
Match: AT1G71350.1 (eukaryotic translation initiation factor SUI1 family protein )

HSP 1 Score: 767.3 bits (1980), Expect = 9.3e-222
Identity = 388/616 (62.99%), Postives = 479/616 (77.76%), Query Frame = 0

Query: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQNRVHIY 60
           MFKKA EAK  QRLSGADRKKL+RTV+ R P  +D  +D +LPPKVE +++KFQNRV +Y
Sbjct: 1   MFKKAVEAKSHQRLSGADRKKLRRTVRNRLPLLTDELLDAILPPKVEITLSKFQNRVIVY 60

Query: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHV 120
           S+EGG PMFFD+DGRG EIFPTVFALW  PE+LP+F LKGGEVSR++IGGADLMFPGI +
Sbjct: 61  SIEGGCPMFFDIDGRGTEIFPTVFALWEAPEMLPSFMLKGGEVSRYIIGGADLMFPGILI 120

Query: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDTLWGL 180
           P +G PSF +GE WAVKVPGN +PIAVG TTMSS EA KAGLRGKALRI HYYRD LW  
Sbjct: 121 PPQGYPSFSAGEIWAVKVPGNLAPIAVGCTTMSSEEARKAGLRGKALRITHYYRDFLWES 180

Query: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETGDATNYGQNDSGNKEVGGSVDET 240
            EGHYVPNAGF+ED V  DP          SY    +G+        +GN+E   +++  
Sbjct: 181 AEGHYVPNAGFMEDVVMEDP----------SYLAPGSGEDILDTSASTGNEEDESALETA 240

Query: 241 TTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNV--QHTLSAEDVDKLLDK 300
           +T   D       A+ D+    +V  +N+L +V+D+   E N   Q TLS E+VD LLD+
Sbjct: 241 STSAAD-------AKNDAEV-HVVGSMNELYLVDDVCATETNTDKQITLSPEEVDTLLDQ 300

Query: 301 CLLQALHITVKDKDFPLPGSTLWSNHVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGLI 360
           CLLQALH T+K+KD P+PGSTLW+NHVLPCRP G+ LDIKKSS+KKLSKWLQ+K+S G+I
Sbjct: 301 CLLQALHTTLKEKDLPIPGSTLWANHVLPCRPSGLTLDIKKSSHKKLSKWLQSKASGGMI 360

Query: 361 TVKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHHGLKESSSNKMLEVAEIY 420
           +VKEDKHKKE VL SVNR HPDY SFKPEK++ E + +S       ++ S K LE+ E+Y
Sbjct: 361 SVKEDKHKKEIVLISVNRSHPDYKSFKPEKKKAE-VSESPGERSTAQAQSEKGLEIIEVY 420

Query: 421 KPSVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLDAILCDSLFK 480
           K S+H + IF +VG D   LYT++EATD+VF+Y+EKE L KPT+KS VVLD ILCD+LFK
Sbjct: 421 KSSIHNSAIFASVGEDKGNLYTASEATDVVFKYIEKENLVKPTNKSMVVLDPILCDALFK 480

Query: 481 GAIKKGTTYPTEIHKRDIGSTFISRMQPHHIVTR-GSESVVRKGALKTIQIMTERRQGNK 540
           GAIKKG+ YP+EIHK+D+GSTF+ RMQP+H+V R G E VVRKGA+K +QIMTERRQGNK
Sbjct: 481 GAIKKGSAYPSEIHKKDVGSTFVGRMQPNHVVMRGGGEPVVRKGAVKPVQIMTERRQGNK 540

Query: 541 KVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQ 600
           KVT+++G+ETFL+D ++  SELQKK ACST+V ELPGKKGYEV++QGGVID+LAR+++E 
Sbjct: 541 KVTKVTGMETFLIDPDSFGSELQKKFACSTSVGELPGKKGYEVLIQGGVIDNLARYMVEH 597

Query: 601 YGIPKKYIEVLDKTRK 614
           YG+PK+YIEVLDKTRK
Sbjct: 601 YGVPKRYIEVLDKTRK 597

BLAST of CSPI02G24770 vs. TAIR 10
Match: AT1G09150.1 (pseudouridine synthase and archaeosine transglycosylase (PUA) domain-containing protein )

HSP 1 Score: 70.5 bits (171), Expect = 5.4e-12
Identity = 51/181 (28.18%), Postives = 91/181 (50.28%), Query Frame = 0

Query: 1   MFKK--ASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQNRVH 60
           MFKK    E   Q ++  + ++++++++++ +P       D LLP K+   V K  N + 
Sbjct: 1   MFKKFCLEEISSQNQVKASVQRRIRQSIQDEYPGLESVMED-LLPKKIPLIVVKCPNHLT 60

Query: 61  IYSVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGI 120
           +  V    P+FF +  R     PT+  L   P ++  F +  G + +FV+ GA++M PG+
Sbjct: 61  LV-VVNNVPLFFCI--RDGPYMPTLRLLHQYPNIMKRFQVDRGAI-KFVLSGANIMCPGL 120

Query: 121 HVPAEGLPSFLSGE-PWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDTL 179
             P   L   +  E P A+   G    +A+G T MS+ + +K+  +G  +  +HY  D L
Sbjct: 121 TSPGGVLDQEVEAERPVAIYAEGKQHALAIGFTKMSAKD-IKSINKGIGVDNMHYLNDGL 175

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5RA636.5e-7131.89Eukaryotic translation initiation factor 2D OS=Pongo abelii OX=9601 GN=EIF2D PE=... [more]
P412141.1e-7031.89Eukaryotic translation initiation factor 2D OS=Homo sapiens OX=9606 GN=EIF2D PE=... [more]
Q612113.6e-6932.10Eukaryotic translation initiation factor 2D OS=Mus musculus OX=10090 GN=Eif2d PE... [more]
P0CL183.6e-6931.77Eukaryotic translation initiation factor 2D OS=Oryctolagus cuniculus OX=9986 GN=... [more]
Q5PPG74.7e-6931.62Eukaryotic translation initiation factor 2D OS=Rattus norvegicus OX=10116 GN=Eif... [more]
Match NameE-valueIdentityDescription
A0A0A0LQM20.0e+0099.67Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G401420 PE=3 SV=1[more]
A0A1S3B3L60.0e+0097.06eukaryotic translation initiation factor 2D isoform X1 OS=Cucumis melo OX=3656 G... [more]
A0A5D3C5R80.0e+0096.87Eukaryotic translation initiation factor 2D isoform X1 OS=Cucumis melo var. maku... [more]
A0A6J1DF372.3e-30889.23eukaryotic translation initiation factor 2D OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1FGK83.8e-30889.23eukaryotic translation initiation factor 2D OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
XP_011649889.10.0e+0099.67eukaryotic translation initiation factor 2D [Cucumis sativus] >XP_031737209.1 eu... [more]
XP_008441190.10.0e+0097.06PREDICTED: eukaryotic translation initiation factor 2D isoform X1 [Cucumis melo][more]
KAA0037038.10.0e+0096.87eukaryotic translation initiation factor 2D isoform X1 [Cucumis melo var. makuwa... [more]
XP_038886638.10.0e+0094.13eukaryotic translation initiation factor 2D [Benincasa hispida] >XP_038886639.1 ... [more]
XP_023550339.12.1e-30889.23eukaryotic translation initiation factor 2D [Cucurbita pepo subsp. pepo] >XP_023... [more]
Match NameE-valueIdentityDescription
AT1G71350.19.3e-22262.99eukaryotic translation initiation factor SUI1 family protein [more]
AT1G09150.15.4e-1228.18pseudouridine synthase and archaeosine transglycosylase (PUA) domain-containing ... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001950SUI1 domainPFAMPF01253SUI1coord: 524..601
e-value: 1.3E-24
score: 86.4
IPR001950SUI1 domainPROSITEPS50296SUI1coord: 526..598
score: 19.194525
IPR041366Pre-PUA domainPFAMPF17832Pre-PUAcoord: 2..90
e-value: 2.6E-19
score: 69.7
NoneNo IPR availableGENE3D3.10.400.20coord: 1..186
e-value: 1.5E-49
score: 170.1
NoneNo IPR availableGENE3D3.30.780.10coord: 522..608
e-value: 4.7E-17
score: 63.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 205..237
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 205..260
NoneNo IPR availablePROSITEPS50890PUAcoord: 93..174
score: 12.839725
NoneNo IPR availableCDDcd11610eIF2D_Ncoord: 9..81
e-value: 8.37054E-19
score: 78.8534
IPR039757Eukaryotic translation initiation factor 2DPANTHERPTHR12217EUKARYOTIC TRANSLATION INITIATION FACTOR 2Dcoord: 1..613
IPR039759Eukaryotic translation initiation factor 2D, SUI1 domainCDDcd11608eIF2D_Ccoord: 526..608
e-value: 7.945E-37
score: 129.991
IPR036877SUI1 domain superfamilySUPERFAMILY55159eIF1-likecoord: 490..609
IPR036885SWIB/MDM2 domain superfamilySUPERFAMILY47592SWIB/MDM2 domaincoord: 407..508
IPR015947PUA-like superfamilySUPERFAMILY88697PUA domain-likecoord: 103..179

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G24770.1CSPI02G24770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001731 formation of translation preinitiation complex
biological_process GO:0006413 translational initiation
molecular_function GO:0003743 translation initiation factor activity