CSPI02G24450 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G24450
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionDEAD-box ATP-dependent RNA helicase FANCM isoform X2
LocationChr2: 20982415 .. 20983203 (-)
RNA-Seq ExpressionCSPI02G24450
SyntenyCSPI02G24450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGTACTAAAATTGTGATCTTATACAACAGGCGTTCTTTGCTAAGTCAATCACCATTTGGGAGGTTGACTTCACCCCTAGCGACCAGGGTAACTGAAAGTGAAACTTCTCCTGACAAGACATTGAATATTTTCCAGTCAACTGTAACAGACGACGTCAATCAATCTCATACACTGCATTCAAAACATGTGAAAATGAACTGCAGCCCAGAAGTTGTAATTGCTACAATTGGTGTCTGCCCAAGGACGACAGACGTGGAAAGCATGAATAGAAATTCGACATTTTGTACATCAGAACCTGTTCCTGTACTTAATTTGGATAAACAGTTTGAGTTGGTGGTGGCAGGGAGAGAATCCATAAGCGAAGTGGATTCAAATAGAAATGTATTCATTGATGATGATGAATTTTATGAAGGTCTTGACCTTGATGCAGTTGAGGCCCATGCAAAATTGCTCCTCCAGAAGAAAGTTGAGTTGCCACAGATTATGGTTACTCAGCAACAGAAGAATATTCCTATCGACACTTCTCCATCTTTTGATCTTGGGATATGACCGTTTTCTATGTGCATATGACTTGTTCAAATACTTAATTTCCGGAAAATATAATCTGGAAAGTTCGGTATTTGTTTTTGGACTCATACTCCTGATTTTCTTCGTGATTGGGGGATATCAAAGTGGCAAGTATGCTTAAATTATGATTTAGTGGTTGGGTGGGGAGGAGTATGTAAAAAACAAATACTACACTGCGTTGTACAAAAGTTATTAAAATAAGAATTTCAGTTTTGTTA

mRNA sequence

ATGTTGTACTAAAATTGTGATCTTATACAACAGGCGTTCTTTGCTAAGTCAATCACCATTTGGGAGGTTGACTTCACCCCTAGCGACCAGGGTAACTGAAAGTGAAACTTCTCCTGACAAGACATTGAATATTTTCCAGTCAACTGTAACAGACGACGTCAATCAATCTCATACACTGCATTCAAAACATGTGAAAATGAACTGCAGCCCAGAAGTTGTAATTGCTACAATTGGTGTCTGCCCAAGGACGACAGACGTGGAAAGCATGAATAGAAATTCGACATTTTGTACATCAGAACCTGTTCCTGTACTTAATTTGGATAAACAGTTTGAGTTGGTGGTGGCAGGGAGAGAATCCATAAGCGAAGTGGATTCAAATAGAAATGTATTCATTGATGATGATGAATTTTATGAAGGTCTTGACCTTGATGCAGTTGAGGCCCATGCAAAATTGCTCCTCCAGAAGAAAGTTGAGTTGCCACAGATTATGGTTACTCAGCAACAGAAGAATATTCCTATCGACACTTCTCCATCTTTTGATCTTGGGATATGACCGTTTTCTATGTGCATATGACTTGTTCAAATACTTAATTTCCGGAAAATATAATCTGGAAAGTTCGGTATTTGTTTTTGGACTCATACTCCTGATTTTCTTCGTGATTGGGGGATATCAAAGTGGCAAGTATGCTTAAATTATGATTTAGTGGTTGGGTGGGGAGGAGTATGTAAAAAACAAATACTACACTGCGTTGTACAAAAGTTATTAAAATAAGAATTTCAGTTTTGTTA

Coding sequence (CDS)

ATGAACTGCAGCCCAGAAGTTGTAATTGCTACAATTGGTGTCTGCCCAAGGACGACAGACGTGGAAAGCATGAATAGAAATTCGACATTTTGTACATCAGAACCTGTTCCTGTACTTAATTTGGATAAACAGTTTGAGTTGGTGGTGGCAGGGAGAGAATCCATAAGCGAAGTGGATTCAAATAGAAATGTATTCATTGATGATGATGAATTTTATGAAGGTCTTGACCTTGATGCAGTTGAGGCCCATGCAAAATTGCTCCTCCAGAAGAAAGTTGAGTTGCCACAGATTATGGTTACTCAGCAACAGAAGAATATTCCTATCGACACTTCTCCATCTTTTGATCTTGGGATATGA

Protein sequence

MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDSNRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI*
Homology
[Close]
BLAST of CSPI02G24450 vs. ExPASy Swiss-Prot
Match: I3XHK1 (DEAD-box ATP-dependent RNA helicase FANCM OS=Arabidopsis thaliana OX=3702 GN=FANCM PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 7.8e-05
Identity = 38/111 (34.23%), Postives = 53/111 (47.75%), Query Frame = 0 

Query: 22   ESMNRNSTFCTSEPVPVLNLDKQF--ELVVAGRESISEVDSNRNVF----IDDDEFYEGL 81
            +S  R  + C S   PV+NL+ +F        +ES   V SN         DDD F+  L
Sbjct: 1233 DSRKRKFSLCNSANAPVINLESKFAAHAQATEKESHEGVRSNAGALEYNDDDDDAFFATL 1292

Query: 82   DLDAVEAHAKLLLQK-------KVELPQIMVTQQQKNIPID-TSPSFDLGI 119
            D DA+EA A LLL K       K +   I     Q++  ++  +PSFDLG+
Sbjct: 1293 DFDAMEAQATLLLSKQRSEAKEKEDATVIPNPGMQRSDGMEKDAPSFDLGL 1343

[Close]
BLAST of CSPI02G24450 vs. ExPASy TrEMBL
Match: A0A0A0LQJ7 (Helicase C-terminal domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G393190 PE=3 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 9.8e-59
Identity = 118/118 (100.00%), Postives = 118/118 (100.00%), Query Frame = 0 

Query: 1   MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS 60
           MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS
Sbjct: 735 MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS 794

Query: 61  NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI 119
           NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI
Sbjct: 795 NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI 852

[Close]
BLAST of CSPI02G24450 vs. ExPASy TrEMBL
Match: A0A5D3C872 (Fanconi anemia group M protein isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G00170 PE=4 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 3.6e-53
Identity = 109/118 (92.37%), Postives = 111/118 (94.07%), Query Frame = 0 

Query: 1   MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS 60
           MNCSPEVVIAT GVCPR TDVES NRNSTFCTSE VPVLNLDKQFELVVAGRESISEVDS
Sbjct: 1   MNCSPEVVIATTGVCPRATDVESRNRNSTFCTSESVPVLNLDKQFELVVAGRESISEVDS 60

Query: 61  NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI 119
           NRNV+IDDDEFYEGLDLDAVEAHAKLLL+KKVELP  MVTQQQKNIPID SPSFDLGI
Sbjct: 61  NRNVYIDDDEFYEGLDLDAVEAHAKLLLEKKVELPLTMVTQQQKNIPIDISPSFDLGI 118

[Close]
BLAST of CSPI02G24450 vs. ExPASy TrEMBL
Match: A0A1S3B3H7 (Fanconi anemia group M protein isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485372 PE=3 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 3.6e-53
Identity = 109/118 (92.37%), Postives = 111/118 (94.07%), Query Frame = 0 

Query: 1    MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS 60
            MNCSPEVVIAT GVCPR TDVES NRNSTFCTSE VPVLNLDKQFELVVAGRESISEVDS
Sbjct: 1198 MNCSPEVVIATTGVCPRATDVESRNRNSTFCTSESVPVLNLDKQFELVVAGRESISEVDS 1257

Query: 61   NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI 119
            NRNV+IDDDEFYEGLDLDAVEAHAKLLL+KKVELP  MVTQQQKNIPID SPSFDLGI
Sbjct: 1258 NRNVYIDDDEFYEGLDLDAVEAHAKLLLEKKVELPLTMVTQQQKNIPIDISPSFDLGI 1315

[Close]
BLAST of CSPI02G24450 vs. ExPASy TrEMBL
Match: A0A1S3B3E0 (Fanconi anemia group M protein isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485372 PE=3 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 3.6e-53
Identity = 109/118 (92.37%), Postives = 111/118 (94.07%), Query Frame = 0 

Query: 1    MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS 60
            MNCSPEVVIAT GVCPR TDVES NRNSTFCTSE VPVLNLDKQFELVVAGRESISEVDS
Sbjct: 1212 MNCSPEVVIATTGVCPRATDVESRNRNSTFCTSESVPVLNLDKQFELVVAGRESISEVDS 1271

Query: 61   NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI 119
            NRNV+IDDDEFYEGLDLDAVEAHAKLLL+KKVELP  MVTQQQKNIPID SPSFDLGI
Sbjct: 1272 NRNVYIDDDEFYEGLDLDAVEAHAKLLLEKKVELPLTMVTQQQKNIPIDISPSFDLGI 1329

[Close]
BLAST of CSPI02G24450 vs. ExPASy TrEMBL
Match: A0A6J1FGG7 (DEAD-box ATP-dependent RNA helicase FANCM isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111445272 PE=3 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 4.0e-36
Identity = 85/118 (72.03%), Postives = 93/118 (78.81%), Query Frame = 0 

Query: 1    MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS 60
            M  SPEVVI+T G CP  T+VES NRN TFC SE VP LNLD+QFELVVAGRESIS+V  
Sbjct: 1227 MKRSPEVVISTTGGCPSATEVESRNRNLTFCASESVPKLNLDRQFELVVAGRESISDV-- 1286

Query: 61   NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI 119
                   DDEFYEGLDLDAVEA AKLLL+KKVELPQ+MVTQ  KN+ + TSPSFDLGI
Sbjct: 1287 -------DDEFYEGLDLDAVEAQAKLLLEKKVELPQMMVTQPHKNLDLHTSPSFDLGI 1335

[Close]
BLAST of CSPI02G24450 vs. NCBI nr
Match: XP_011649875.1 (DEAD-box ATP-dependent RNA helicase FANCM isoform X1 [Cucumis sativus])

HSP 1 Score: 235.7 bits (600), Expect = 2.0e-58
Identity = 118/118 (100.00%), Postives = 118/118 (100.00%), Query Frame = 0 

Query: 1    MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS 60
            MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS
Sbjct: 1211 MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS 1270

Query: 61   NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI 119
            NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI
Sbjct: 1271 NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI 1328

[Close]
BLAST of CSPI02G24450 vs. NCBI nr
Match: XP_031736810.1 (DEAD-box ATP-dependent RNA helicase FANCM isoform X5 [Cucumis sativus])

HSP 1 Score: 235.7 bits (600), Expect = 2.0e-58
Identity = 118/118 (100.00%), Postives = 118/118 (100.00%), Query Frame = 0 

Query: 1    MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS 60
            MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS
Sbjct: 1114 MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS 1173

Query: 61   NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI 119
            NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI
Sbjct: 1174 NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI 1231

[Close]
BLAST of CSPI02G24450 vs. NCBI nr
Match: XP_031736807.1 (DEAD-box ATP-dependent RNA helicase FANCM isoform X2 [Cucumis sativus])

HSP 1 Score: 235.7 bits (600), Expect = 2.0e-58
Identity = 118/118 (100.00%), Postives = 118/118 (100.00%), Query Frame = 0 

Query: 1    MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS 60
            MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS
Sbjct: 1182 MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS 1241

Query: 61   NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI 119
            NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI
Sbjct: 1242 NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI 1299

[Close]
BLAST of CSPI02G24450 vs. NCBI nr
Match: XP_031736808.1 (DEAD-box ATP-dependent RNA helicase FANCM isoform X3 [Cucumis sativus])

HSP 1 Score: 235.7 bits (600), Expect = 2.0e-58
Identity = 118/118 (100.00%), Postives = 118/118 (100.00%), Query Frame = 0 

Query: 1    MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS 60
            MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS
Sbjct: 1163 MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS 1222

Query: 61   NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI 119
            NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI
Sbjct: 1223 NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI 1280

[Close]
BLAST of CSPI02G24450 vs. NCBI nr
Match: XP_031736811.1 (DEAD-box ATP-dependent RNA helicase FANCM isoform X6 [Cucumis sativus])

HSP 1 Score: 235.7 bits (600), Expect = 2.0e-58
Identity = 118/118 (100.00%), Postives = 118/118 (100.00%), Query Frame = 0 

Query: 1    MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS 60
            MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS
Sbjct: 1082 MNCSPEVVIATIGVCPRTTDVESMNRNSTFCTSEPVPVLNLDKQFELVVAGRESISEVDS 1141

Query: 61   NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI 119
            NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI
Sbjct: 1142 NRNVFIDDDEFYEGLDLDAVEAHAKLLLQKKVELPQIMVTQQQKNIPIDTSPSFDLGI 1199

[Close]
BLAST of CSPI02G24450 vs. TAIR 10
Match: AT1G35530.1 (DEAD/DEAH box RNA helicase family protein )

HSP 1 Score: 48.1 bits (113), Expect = 5.6e-06
Identity = 38/111 (34.23%), Postives = 53/111 (47.75%), Query Frame = 0 

Query: 22   ESMNRNSTFCTSEPVPVLNLDKQF--ELVVAGRESISEVDSNRNVF----IDDDEFYEGL 81
            +S  R  + C S   PV+NL+ +F        +ES   V SN         DDD F+  L
Sbjct: 1213 DSRKRKFSLCNSANAPVINLESKFAAHAQATEKESHEGVRSNAGALEYNDDDDDAFFATL 1272

Query: 82   DLDAVEAHAKLLLQK-------KVELPQIMVTQQQKNIPID-TSPSFDLGI 119
            D DA+EA A LLL K       K +   I     Q++  ++  +PSFDLG+
Sbjct: 1273 DFDAMEAQATLLLSKQRSEAKEKEDATVIPNPGMQRSDGMEKDAPSFDLGL 1323

[Close]
BLAST of CSPI02G24450 vs. TAIR 10
Match: AT1G35530.2 (DEAD/DEAH box RNA helicase family protein )

HSP 1 Score: 48.1 bits (113), Expect = 5.6e-06
Identity = 38/111 (34.23%), Postives = 53/111 (47.75%), Query Frame = 0 

Query: 22   ESMNRNSTFCTSEPVPVLNLDKQF--ELVVAGRESISEVDSNRNVF----IDDDEFYEGL 81
            +S  R  + C S   PV+NL+ +F        +ES   V SN         DDD F+  L
Sbjct: 1279 DSRKRKFSLCNSANAPVINLESKFAAHAQATEKESHEGVRSNAGALEYNDDDDDAFFATL 1338

Query: 82   DLDAVEAHAKLLLQK-------KVELPQIMVTQQQKNIPID-TSPSFDLGI 119
            D DA+EA A LLL K       K +   I     Q++  ++  +PSFDLG+
Sbjct: 1339 DFDAMEAQATLLLSKQRSEAKEKEDATVIPNPGMQRSDGMEKDAPSFDLGL 1389

The following BLAST results are available for this feature:
BLAST of CSPI02G24450 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
I3XHK17.8e-0534.23DEAD-box ATP-dependent RNA helicase FANCM OS=Arabidopsis thaliana OX=3702 GN=FAN... [more]
BLAST of CSPI02G24450 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LQJ79.8e-59100.00Helicase C-terminal domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_... [more]
A0A5D3C8723.6e-5392.37Fanconi anemia group M protein isoform X2 OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3B3H73.6e-5392.37Fanconi anemia group M protein isoform X2 OS=Cucumis melo OX=3656 GN=LOC10348537... [more]
A0A1S3B3E03.6e-5392.37Fanconi anemia group M protein isoform X1 OS=Cucumis melo OX=3656 GN=LOC10348537... [more]
A0A6J1FGG74.0e-3672.03DEAD-box ATP-dependent RNA helicase FANCM isoform X4 OS=Cucurbita moschata OX=36... [more]
BLAST of CSPI02G24450 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_011649875.12.0e-58100.00DEAD-box ATP-dependent RNA helicase FANCM isoform X1 [Cucumis sativus][more]
XP_031736810.12.0e-58100.00DEAD-box ATP-dependent RNA helicase FANCM isoform X5 [Cucumis sativus][more]
XP_031736807.12.0e-58100.00DEAD-box ATP-dependent RNA helicase FANCM isoform X2 [Cucumis sativus][more]
XP_031736808.12.0e-58100.00DEAD-box ATP-dependent RNA helicase FANCM isoform X3 [Cucumis sativus][more]
XP_031736811.12.0e-58100.00DEAD-box ATP-dependent RNA helicase FANCM isoform X6 [Cucumis sativus][more]
BLAST of CSPI02G24450 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G35530.15.6e-0634.23DEAD/DEAH box RNA helicase family protein [more]
AT1G35530.25.6e-0634.23DEAD/DEAH box RNA helicase family protein [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G24450.1CSPI02G24450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032508 DNA duplex unwinding
biological_process GO:0006281 DNA repair
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0043138 3'-5' DNA helicase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0016787 hydrolase activity