CSPI02G23860 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G23860
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPoly(A)-specific ribonuclease
LocationChr2: 20470477 .. 20472323 (-)
RNA-Seq ExpressionCSPI02G23860
SyntenyCSPI02G23860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACAGCTGAAAGGATAAGAATTGATTTTTGAAAAAAAAAAACGAAAAGAAAAATGAAACAATTATTGTGGGAAGGGAAAAAAGAGTAAGAAGATAGCCCAAAGGGAAATGCGAACGGCGTCGTATAAGGGAAAACGACATGGTTTGGGAGGTGTGTGTGAATCCATTCATCATCGTCATGTTGGTGATGATACCGGGATGAAGCAAAATTTAGGGCGGGTCGAAGCGGAGCTCTCATCCCCATTTGTTTGTGTGCTCCATTGCCTCTCCGTCCAACACTTCTCTGCCTTTTAAGATCTCTCTGTTTCAATTCCTTCAAGGTTAGATCCCTTCGCCTCACTCTTGGAGGGGGTTTCTCCCCCCATTTCCCCCTCGATTCTTTTTCCTTTTCCTTTTCCTTTTCTTTATCATCTAGGGTTCTATCCAATCCTCACTCTCTTTCTTTTCTGCTTCACTCTCCCCCTGATCTTCTAGATCCAGGAAGATTTCAATTCTTTCTCACGGCCCCACGTTACCTTTCAAGGGCCAAACCCCGAACCCCTTTTCTTGTTTTTCTTTTATTTTCCCCATTTCTTGTTCTTGGGGGTTTTTTCTCTCTTTTGCCATTTTGTTGGTTTTGTTGACGTTCGAGGTATGTCGATTTTGCCGAAAGGTGAATCGATTAATATCCGTGAGGTGTGGAACGATAATTTGGAGGAAGAATTTGCTCTTATACGTGATATTGTCGACCAGTTTAACTACATAGCGATGGACACCGAGTTCCCTGGCGTGGTTGTTCGTCCTGTGGGTTCTTTTAAGAATATTAATGAGTATAACTACCGAACCCTCAGAGAGAATGTTGATACCTTGAAGTTGATTCAATTGGGTCTTACGTTTTCTGATGAGAATGGGAATCTCCCAACTTGTGGAACAGATAAGTTTTGTATTTGGCAGTTCAATTTCCGCGAGTTCAATATTTGTGATGATATTTATGCAAGTGATTCAATTGAGCTTTTGCGCGAGTGTGGTATTGATTTTCAGAAGACCCATGAGGAGGGAATTGATGTCAATCGCTTTGGTGAGCTTCTCATGTCATCTGGGATTGTGTTAAACGACAATATTTTTTGGGTGACGTTCCACAGCGGGTATGATTTTGGGTACTTGTTGAAATTGTTGACTTGCCGAAACTTACCAGAGACCCAGGCCGAATTTTTTGATTTGATCCATATGTACTTTCCGATGGTCTATGATATCAAGCATTTGATGAGGTTTTGCAACAGTTTCCATGGTGGGTTGAACAAGCTTGCAGAGTTACTAGAAGTGGAGAGGATTGGAGTGTGCCATCAAGCAGGATCAGATAGCCTCCTTACTGCCTGTACTTTCAGGAAGTTGAGGGATACTTTTTTCAATGGTTCTACACAGAAATATGCAGGTGTTTTATACGGTCTTGGTGTTGAAACTGGACAGACTACCAATTGAAGACACTTCAAGTGTTTGTAGCATTGTGAAACTTGGTTAAAGCATTGATGGTTGTATCTGTTTTTCTTTTTCGCTTGTAAATTATGGCAGTTGGCAGTTAGTATTTCAATAAAACCTTAATGGCACTCTTTCAGTTAGTTAACTTGTTTTGTTAGATGCAATGCTATATTTTACGTGCCTTTCCATTCAAATCTGTTATACGTCAAAGATAGATGGATGAATACAATAGTGGTGGCAATATAGAACATATTTCTTGAGATACTGTGTGCCTTATTATTTGATTTCTGAAATTAAATTTTCTTCAATTGGCCTGTTGCTATGACCATTCATTCTTCTTCCCGTTGATGCCGCCATATCTATACCAACTACTGACGATTGTTTGACAAGAC

mRNA sequence

AAACAGCTGAAAGGATAAGAATTGATTTTTGAAAAAAAAAAACGAAAAGAAAAATGAAACAATTATTGTGGGAAGGGAAAAAAGAGTAAGAAGATAGCCCAAAGGGAAATGCGAACGGCGTCGTATAAGGGAAAACGACATGGTTTGGGAGGTGTGTGTGAATCCATTCATCATCGTCATGTTGGTGATGATACCGGGATGAAGCAAAATTTAGGGCGGGTCGAAGCGGAGCTCTCATCCCCATTTGTTTGTGTGCTCCATTGCCTCTCCGTCCAACACTTCTCTGCCTTTTAAGATCTCTCTGTTTCAATTCCTTCAAGGTTAGATCCCTTCGCCTCACTCTTGGAGGGGGTTTCTCCCCCCATTTCCCCCTCGATTCTTTTTCCTTTTCCTTTTCCTTTTCTTTATCATCTAGGGTTCTATCCAATCCTCACTCTCTTTCTTTTCTGCTTCACTCTCCCCCTGATCTTCTAGATCCAGGAAGATTTCAATTCTTTCTCACGGCCCCACGTTACCTTTCAAGGGCCAAACCCCGAACCCCTTTTCTTGTTTTTCTTTTATTTTCCCCATTTCTTGTTCTTGGGGGTTTTTTCTCTCTTTTGCCATTTTGTTGGTTTTGTTGACGTTCGAGGTATGTCGATTTTGCCGAAAGGTGAATCGATTAATATCCGTGAGGTGTGGAACGATAATTTGGAGGAAGAATTTGCTCTTATACGTGATATTGTCGACCAGTTTAACTACATAGCGATGGACACCGAGTTCCCTGGCGTGGTTGTTCGTCCTGTGGGTTCTTTTAAGAATATTAATGAGTATAACTACCGAACCCTCAGAGAGAATGTTGATACCTTGAAGTTGATTCAATTGGGTCTTACGTTTTCTGATGAGAATGGGAATCTCCCAACTTGTGGAACAGATAAGTTTTGTATTTGGCAGTTCAATTTCCGCGAGTTCAATATTTGTGATGATATTTATGCAAGTGATTCAATTGAGCTTTTGCGCGAGTGTGGTATTGATTTTCAGAAGACCCATGAGGAGGGAATTGATGTCAATCGCTTTGGTGAGCTTCTCATGTCATCTGGGATTGTGTTAAACGACAATATTTTTTGGGTGACGTTCCACAGCGGGTATGATTTTGGGTACTTGTTGAAATTGTTGACTTGCCGAAACTTACCAGAGACCCAGGCCGAATTTTTTGATTTGATCCATATGTACTTTCCGATGGTCTATGATATCAAGCATTTGATGAGGTTTTGCAACAGTTTCCATGGTGGGTTGAACAAGCTTGCAGAGTTACTAGAAGTGGAGAGGATTGGAGTGTGCCATCAAGCAGGATCAGATAGCCTCCTTACTGCCTGTACTTTCAGGAAGTTGAGGGATACTTTTTTCAATGGTTCTACACAGAAATATGCAGGTGTTTTATACGGTCTTGGTGTTGAAACTGGACAGACTACCAATTGAAGACACTTCAAGTGTTTGTAGCATTGTGAAACTTGGTTAAAGCATTGATGGTTGTATCTGTTTTTCTTTTTCGCTTGTAAATTATGGCAGTTGGCAGTTAGTATTTCAATAAAACCTTAATGGCACTCTTTCAGTTAGTTAACTTGTTTTGTTAGATGCAATGCTATATTTTACGTGCCTTTCCATTCAAATCTGTTATACGTCAAAGATAGATGGATGAATACAATAGTGGTGGCAATATAGAACATATTTCTTGAGATACTGTGTGCCTTATTATTTGATTTCTGAAATTAAATTTTCTTCAATTGGCCTGTTGCTATGACCATTCATTCTTCTTCCCGTTGATGCCGCCATATCTATACCAACTACTGACGATTGTTTGACAAGAC

Coding sequence (CDS)

ATGTCGATTTTGCCGAAAGGTGAATCGATTAATATCCGTGAGGTGTGGAACGATAATTTGGAGGAAGAATTTGCTCTTATACGTGATATTGTCGACCAGTTTAACTACATAGCGATGGACACCGAGTTCCCTGGCGTGGTTGTTCGTCCTGTGGGTTCTTTTAAGAATATTAATGAGTATAACTACCGAACCCTCAGAGAGAATGTTGATACCTTGAAGTTGATTCAATTGGGTCTTACGTTTTCTGATGAGAATGGGAATCTCCCAACTTGTGGAACAGATAAGTTTTGTATTTGGCAGTTCAATTTCCGCGAGTTCAATATTTGTGATGATATTTATGCAAGTGATTCAATTGAGCTTTTGCGCGAGTGTGGTATTGATTTTCAGAAGACCCATGAGGAGGGAATTGATGTCAATCGCTTTGGTGAGCTTCTCATGTCATCTGGGATTGTGTTAAACGACAATATTTTTTGGGTGACGTTCCACAGCGGGTATGATTTTGGGTACTTGTTGAAATTGTTGACTTGCCGAAACTTACCAGAGACCCAGGCCGAATTTTTTGATTTGATCCATATGTACTTTCCGATGGTCTATGATATCAAGCATTTGATGAGGTTTTGCAACAGTTTCCATGGTGGGTTGAACAAGCTTGCAGAGTTACTAGAAGTGGAGAGGATTGGAGTGTGCCATCAAGCAGGATCAGATAGCCTCCTTACTGCCTGTACTTTCAGGAAGTTGAGGGATACTTTTTTCAATGGTTCTACACAGAAATATGCAGGTGTTTTATACGGTCTTGGTGTTGAAACTGGACAGACTACCAATTGA

Protein sequence

MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEYNYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIELLRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLPETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTACTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN*
Homology
BLAST of CSPI02G23860 vs. ExPASy Swiss-Prot
Match: Q9SKZ2 (Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=CAF1-7 PE=2 SV=2)

HSP 1 Score: 439.1 bits (1128), Expect = 3.7e-122
Identity = 203/272 (74.63%), Postives = 236/272 (86.76%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MS+  K +SI IREVWNDNLE E ALIR++VD F ++AMDTEFPG+V RPVG+FK   EY
Sbjct: 1   MSLFLKDDSIQIREVWNDNLESEMALIREVVDDFPFVAMDTEFPGIVCRPVGTFKTNTEY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTD-KFCIWQFNFREFNICDDIYASDSIE 120
           +Y TL+ NV+ LK+IQLGLTFSDE GNLPTCGTD K+CIWQFNFREF++  DIYA+DSIE
Sbjct: 61  HYETLKTNVNILKMIQLGLTFSDEKGNLPTCGTDNKYCIWQFNFREFDLESDIYATDSIE 120

Query: 121 LLRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNL 180
           LLR+ GIDF K +E GID  RF ELLMSSGIVLN+N+ WVTFHSGYDFGYLLKLLTC+NL
Sbjct: 121 LLRQSGIDFVKNNEFGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNL 180

Query: 181 PETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLT 240
           PETQ  FF++I +YFP VYDIKHLM+FCNS HGGLNKLAELL+VER+G+CHQAGSDSLLT
Sbjct: 181 PETQTGFFEMISVYFPRVYDIKHLMKFCNSLHGGLNKLAELLDVERVGICHQAGSDSLLT 240

Query: 241 ACTFRKLRDTFFNGSTQKYAGVLYGLGVETGQ 272
           +CTFRKL++ FF GS +KY+GVLYGLGVE GQ
Sbjct: 241 SCTFRKLQENFFIGSMEKYSGVLYGLGVENGQ 272

BLAST of CSPI02G23860 vs. ExPASy Swiss-Prot
Match: Q9SAI2 (Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=CAF1-6 PE=1 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 1.2e-120
Identity = 198/271 (73.06%), Postives = 234/271 (86.35%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MS+  K +SI IREVWNDNL+EE  LIRD+VD F Y+AMDTEFPG+VVRPVG+FK+  +Y
Sbjct: 1   MSLFLKDDSIQIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRPVGTFKSNADY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           +Y TL+ NV+ LK+IQLGLTFS+E GNLPTCGTDK+CIWQFNFREF++  DI+A DSIEL
Sbjct: 61  HYETLKTNVNILKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180
           L++ GID  K   +GID  RF ELLMSSGIVLN+N+ WVTFHSGYDFGYLLKLLTC+NLP
Sbjct: 121 LKQSGIDLAKNTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240
           ++Q +FF LI++YFP VYDIKHLM+FCNS HGGLNKLAELLEVER+G+CHQAGSDSLLT+
Sbjct: 181 DSQTDFFKLINVYFPTVYDIKHLMKFCNSLHGGLNKLAELLEVERVGICHQAGSDSLLTS 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQ 272
           CTFRKL++ FF G   KY+GVLYGLGVE GQ
Sbjct: 241 CTFRKLKENFFVGPLHKYSGVLYGLGVENGQ 271

BLAST of CSPI02G23860 vs. ExPASy Swiss-Prot
Match: Q9LEU4 (Probable CCR4-associated factor 1 homolog 10 OS=Arabidopsis thaliana OX=3702 GN=CAF1-10 PE=2 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 2.0e-120
Identity = 203/268 (75.75%), Postives = 235/268 (87.69%), Query Frame = 0

Query: 6   KGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEYNYRTL 65
           K +SI IREVW+ NL EEFALIR+IVD+F+YIAMDTEFPGVV++PV +FK  N+ NYRTL
Sbjct: 6   KEDSIMIREVWDYNLVEEFALIREIVDKFSYIAMDTEFPGVVLKPVATFKYNNDLNYRTL 65

Query: 66  RENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIELLRECG 125
           +ENVD LKLIQ+GLTFSDENGNLPTCGTDKFCIWQFNFREFNI +DIYAS+SIELLR+CG
Sbjct: 66  KENVDLLKLIQVGLTFSDENGNLPTCGTDKFCIWQFNFREFNIGEDIYASESIELLRQCG 125

Query: 126 IDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLPETQAE 185
           IDF+K  E+GIDV RFGEL+MSSGIVLND I WVTFH GYDFGYL+KLLTC+ LP  QA+
Sbjct: 126 IDFKKNIEKGIDVVRFGELMMSSGIVLNDAISWVTFHGGYDFGYLVKLLTCKELPLKQAD 185

Query: 186 FFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTACTFRK 245
           FF L+++YFP VYDIKHLM FCN   GGLN+LAEL+ VER+G+CHQAGSDSLLT  +FRK
Sbjct: 186 FFKLLYVYFPTVYDIKHLMTFCNGLFGGLNRLAELMGVERVGICHQAGSDSLLTLGSFRK 245

Query: 246 LRDTFFNGSTQKYAGVLYGLGVETGQTT 274
           L++ +F GST+KY GVLYGLGVE G TT
Sbjct: 246 LKERYFPGSTEKYTGVLYGLGVEDGTTT 273

BLAST of CSPI02G23860 vs. ExPASy Swiss-Prot
Match: Q9S9P2 (Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=CAF1-2 PE=1 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 2.5e-94
Identity = 164/264 (62.12%), Postives = 202/264 (76.52%), Query Frame = 0

Query: 8   ESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNIN------EYN 67
           ++I IREVWN NLE+E ALI   +D F Y+AMDTEFPG+V + V +  N N      EYN
Sbjct: 12  DTIEIREVWNHNLEQEMALIEQSIDDFPYVAMDTEFPGIVCKTVTANPNPNPYSIHYEYN 71

Query: 68  YRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIELL 127
           Y TL+ NV+ LKLIQLGLT SDE GNLPTCGT+K CIWQFNFREFN+  D++A DSIELL
Sbjct: 72  YDTLKANVNMLKLIQLGLTLSDEKGNLPTCGTNKQCIWQFNFREFNVISDMFALDSIELL 131

Query: 128 RECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLPE 187
           R+  ID +K +E G+D  RF ELLM SG+VLND I WVTFH GYDFGYLLKLL+ + LPE
Sbjct: 132 RKSAIDLEKNNECGVDAKRFAELLMGSGVVLNDKIHWVTFHCGYDFGYLLKLLSGKELPE 191

Query: 188 TQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTAC 247
             ++FFD +  +FP+VYDIK+LM FC + +GGL K+AELL V+R+G+ HQAGSDSLLT  
Sbjct: 192 EISDFFDQMEKFFPVVYDIKYLMGFCTNLYGGLEKIAELLGVKRVGISHQAGSDSLLTLR 251

Query: 248 TFRKLRDTFFNGSTQKYAGVLYGL 266
           TF K+++ FF GS  KY+G L+GL
Sbjct: 252 TFIKMKEFFFTGSLLKYSGFLFGL 275

BLAST of CSPI02G23860 vs. ExPASy Swiss-Prot
Match: Q3ZC01 (CCR4-NOT transcription complex subunit 7 OS=Bos taurus OX=9913 GN=CNOT7 PE=2 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 5.0e-79
Identity = 144/268 (53.73%), Postives = 190/268 (70.90%), Query Frame = 0

Query: 9   SINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEYNYRTLREN 68
           S  I EVW  NL+EE   IR ++ ++NY+AMDTEFPGVV RP+G F++  +Y Y+ LR N
Sbjct: 9   SQRICEVWACNLDEEMKKIRQVIRKYNYVAMDTEFPGVVARPIGEFRSNADYQYQLLRCN 68

Query: 69  VDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIELLRECGIDF 128
           VD LK+IQLGLTF +E G  P  GT     WQFNF+ FN+ +D+YA DSIELL   GI F
Sbjct: 69  VDLLKIIQLGLTFMNEQGEYPP-GTS---TWQFNFK-FNLTEDMYAQDSIELLTTSGIQF 128

Query: 129 QKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLPETQAEFFD 188
           +K  EEGI+   F ELLM+SG+VL + + W++FHSGYDFGYL+K+LT  NLPE + +FF+
Sbjct: 129 KKHEEEGIETQYFAELLMTSGVVLCEGVKWLSFHSGYDFGYLIKILTNSNLPEEELDFFE 188

Query: 189 LIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTACTFRKLRD 248
           ++ ++FP++YD+K+LM+ C +  GGL ++AE LE+ERIG  HQAGSDSLLT   F K+R+
Sbjct: 189 ILRLFFPVIYDVKYLMKSCKNLKGGLQEVAEQLELERIGPQHQAGSDSLLTGMAFFKMRE 248

Query: 249 TFFNG--STQKYAGVLYGLGVETGQTTN 275
            FF       KY G LYGLG  +    N
Sbjct: 249 MFFEDHIDDAKYCGHLYGLGSGSSYVQN 271

BLAST of CSPI02G23860 vs. ExPASy TrEMBL
Match: A0A0A0LSM4 (Poly(A)-specific ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_2G382590 PE=3 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 5.4e-161
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY
Sbjct: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL
Sbjct: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180
           LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP
Sbjct: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240
           ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA
Sbjct: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 275
           CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN
Sbjct: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 274

BLAST of CSPI02G23860 vs. ExPASy TrEMBL
Match: A0A5A7VC99 (Poly(A)-specific ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002090 PE=3 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 2.7e-160
Identity = 273/274 (99.64%), Postives = 273/274 (99.64%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY
Sbjct: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL
Sbjct: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180
           LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP
Sbjct: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240
           ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA
Sbjct: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 275
           CTFRKLRDTFFNGS QKYAGVLYGLGVETGQTTN
Sbjct: 241 CTFRKLRDTFFNGSIQKYAGVLYGLGVETGQTTN 274

BLAST of CSPI02G23860 vs. ExPASy TrEMBL
Match: A0A1S3BD02 (Poly(A)-specific ribonuclease OS=Cucumis melo OX=3656 GN=LOC103488343 PE=3 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 1.7e-159
Identity = 272/274 (99.27%), Postives = 272/274 (99.27%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY
Sbjct: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL
Sbjct: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180
           LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNI WVTFHSGYDFGYLLKLLTCRNLP
Sbjct: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIXWVTFHSGYDFGYLLKLLTCRNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240
           ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA
Sbjct: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 275
           CTFRKLRDTFFNGS QKYAGVLYGLGVETGQTTN
Sbjct: 241 CTFRKLRDTFFNGSIQKYAGVLYGLGVETGQTTN 274

BLAST of CSPI02G23860 vs. ExPASy TrEMBL
Match: A0A6J1KLE0 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111496296 PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.4e-148
Identity = 250/274 (91.24%), Postives = 268/274 (97.81%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESI+IREVWN N+EEEFAL+R+IVDQFNYIAMDTEFPGVVVRPVG+FKNIN+Y
Sbjct: 1   MSILPKGESIHIREVWNHNVEEEFALMREIVDQFNYIAMDTEFPGVVVRPVGAFKNINDY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NY+TL++NVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNI +DI+ASDSIEL
Sbjct: 61  NYQTLKDNVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNISNDIFASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180
           LR+CGIDFQK +EEGIDVN FGELLMSSGIVLNDN++WVTFHSGYDFGYLLKLLTCR+LP
Sbjct: 121 LRQCGIDFQKNNEEGIDVNHFGELLMSSGIVLNDNVYWVTFHSGYDFGYLLKLLTCRSLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240
            TQA FFDLI+MYFPMVYDIKHLM+FCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA
Sbjct: 181 GTQAAFFDLINMYFPMVYDIKHLMKFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 275
           CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN
Sbjct: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 274

BLAST of CSPI02G23860 vs. ExPASy TrEMBL
Match: A0A6J1GJJ8 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111454439 PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.4e-148
Identity = 250/274 (91.24%), Postives = 268/274 (97.81%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESI+IREVWN N+EEEFAL+R+IVDQFNYIAMDTEFPGVVVRPVG+FKNIN+Y
Sbjct: 1   MSILPKGESIHIREVWNHNVEEEFALMREIVDQFNYIAMDTEFPGVVVRPVGAFKNINDY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NY+TL++NVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNI +DI+ASDSIEL
Sbjct: 61  NYQTLKDNVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNISNDIFASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180
           LR+CGIDFQK +EEGIDVN FGELLMSSGIVLNDN++WVTFHSGYDFGYLLKLLTCR+LP
Sbjct: 121 LRQCGIDFQKNNEEGIDVNHFGELLMSSGIVLNDNVYWVTFHSGYDFGYLLKLLTCRSLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240
            TQA FFDLI+MYFPMVYDIKHLM+FCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA
Sbjct: 181 GTQAAFFDLINMYFPMVYDIKHLMKFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 275
           CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN
Sbjct: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 274

BLAST of CSPI02G23860 vs. NCBI nr
Match: XP_004138706.1 (probable CCR4-associated factor 1 homolog 7 [Cucumis sativus] >KGN62986.1 hypothetical protein Csa_021871 [Cucumis sativus])

HSP 1 Score: 576.6 bits (1485), Expect = 1.1e-160
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY
Sbjct: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL
Sbjct: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180
           LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP
Sbjct: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240
           ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA
Sbjct: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 275
           CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN
Sbjct: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 274

BLAST of CSPI02G23860 vs. NCBI nr
Match: KAA0064894.1 (putative CCR4-associated factor 1-like protein 7 [Cucumis melo var. makuwa])

HSP 1 Score: 574.3 bits (1479), Expect = 5.5e-160
Identity = 273/274 (99.64%), Postives = 273/274 (99.64%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY
Sbjct: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL
Sbjct: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180
           LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP
Sbjct: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240
           ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA
Sbjct: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 275
           CTFRKLRDTFFNGS QKYAGVLYGLGVETGQTTN
Sbjct: 241 CTFRKLRDTFFNGSIQKYAGVLYGLGVETGQTTN 274

BLAST of CSPI02G23860 vs. NCBI nr
Match: XP_008445257.1 (PREDICTED: LOW QUALITY PROTEIN: probable CCR4-associated factor 1 homolog 7 [Cucumis melo])

HSP 1 Score: 571.6 bits (1472), Expect = 3.6e-159
Identity = 272/274 (99.27%), Postives = 272/274 (99.27%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY
Sbjct: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL
Sbjct: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180
           LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNI WVTFHSGYDFGYLLKLLTCRNLP
Sbjct: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIXWVTFHSGYDFGYLLKLLTCRNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240
           ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA
Sbjct: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 275
           CTFRKLRDTFFNGS QKYAGVLYGLGVETGQTTN
Sbjct: 241 CTFRKLRDTFFNGSIQKYAGVLYGLGVETGQTTN 274

BLAST of CSPI02G23860 vs. NCBI nr
Match: XP_038886120.1 (probable CCR4-associated factor 1 homolog 7 [Benincasa hispida])

HSP 1 Score: 558.5 bits (1438), Expect = 3.1e-155
Identity = 264/274 (96.35%), Postives = 271/274 (98.91%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESINIREVWN NLEEEFALIR+IVDQFNYIAMDTEFPGVVVRPVGSFKNINEY
Sbjct: 1   MSILPKGESINIREVWNHNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NYRTL+ENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNIC+DIYASDSIEL
Sbjct: 61  NYRTLKENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICEDIYASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180
           LRECGIDF+KT+EEGIDVNRF ELLMSSGIVLNDNI+WVTFHSGYDFGYLLKLLTCRNLP
Sbjct: 121 LRECGIDFKKTNEEGIDVNRFAELLMSSGIVLNDNIYWVTFHSGYDFGYLLKLLTCRNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240
           +TQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA
Sbjct: 181 QTQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 275
           CTFRKLRDTFFNGSTQKYAGVLYGLGVE GQTTN
Sbjct: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVEPGQTTN 274

BLAST of CSPI02G23860 vs. NCBI nr
Match: XP_022951690.1 (probable CCR4-associated factor 1 homolog 7 [Cucurbita moschata] >XP_023002466.1 probable CCR4-associated factor 1 homolog 7 [Cucurbita maxima] >KAG6585496.1 putative CCR4-associated factor 1-like 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 534.6 bits (1376), Expect = 4.9e-148
Identity = 250/274 (91.24%), Postives = 268/274 (97.81%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESI+IREVWN N+EEEFAL+R+IVDQFNYIAMDTEFPGVVVRPVG+FKNIN+Y
Sbjct: 1   MSILPKGESIHIREVWNHNVEEEFALMREIVDQFNYIAMDTEFPGVVVRPVGAFKNINDY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NY+TL++NVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNI +DI+ASDSIEL
Sbjct: 61  NYQTLKDNVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNISNDIFASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180
           LR+CGIDFQK +EEGIDVN FGELLMSSGIVLNDN++WVTFHSGYDFGYLLKLLTCR+LP
Sbjct: 121 LRQCGIDFQKNNEEGIDVNHFGELLMSSGIVLNDNVYWVTFHSGYDFGYLLKLLTCRSLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240
            TQA FFDLI+MYFPMVYDIKHLM+FCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA
Sbjct: 181 GTQAAFFDLINMYFPMVYDIKHLMKFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 275
           CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN
Sbjct: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 274

BLAST of CSPI02G23860 vs. TAIR 10
Match: AT2G32070.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 439.1 bits (1128), Expect = 2.6e-123
Identity = 203/272 (74.63%), Postives = 236/272 (86.76%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MS+  K +SI IREVWNDNLE E ALIR++VD F ++AMDTEFPG+V RPVG+FK   EY
Sbjct: 1   MSLFLKDDSIQIREVWNDNLESEMALIREVVDDFPFVAMDTEFPGIVCRPVGTFKTNTEY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTD-KFCIWQFNFREFNICDDIYASDSIE 120
           +Y TL+ NV+ LK+IQLGLTFSDE GNLPTCGTD K+CIWQFNFREF++  DIYA+DSIE
Sbjct: 61  HYETLKTNVNILKMIQLGLTFSDEKGNLPTCGTDNKYCIWQFNFREFDLESDIYATDSIE 120

Query: 121 LLRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNL 180
           LLR+ GIDF K +E GID  RF ELLMSSGIVLN+N+ WVTFHSGYDFGYLLKLLTC+NL
Sbjct: 121 LLRQSGIDFVKNNEFGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNL 180

Query: 181 PETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLT 240
           PETQ  FF++I +YFP VYDIKHLM+FCNS HGGLNKLAELL+VER+G+CHQAGSDSLLT
Sbjct: 181 PETQTGFFEMISVYFPRVYDIKHLMKFCNSLHGGLNKLAELLDVERVGICHQAGSDSLLT 240

Query: 241 ACTFRKLRDTFFNGSTQKYAGVLYGLGVETGQ 272
           +CTFRKL++ FF GS +KY+GVLYGLGVE GQ
Sbjct: 241 SCTFRKLQENFFIGSMEKYSGVLYGLGVENGQ 272

BLAST of CSPI02G23860 vs. TAIR 10
Match: AT1G80780.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 434.1 bits (1115), Expect = 8.3e-122
Identity = 198/271 (73.06%), Postives = 234/271 (86.35%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MS+  K +SI IREVWNDNL+EE  LIRD+VD F Y+AMDTEFPG+VVRPVG+FK+  +Y
Sbjct: 1   MSLFLKDDSIQIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRPVGTFKSNADY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           +Y TL+ NV+ LK+IQLGLTFS+E GNLPTCGTDK+CIWQFNFREF++  DI+A DSIEL
Sbjct: 61  HYETLKTNVNILKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180
           L++ GID  K   +GID  RF ELLMSSGIVLN+N+ WVTFHSGYDFGYLLKLLTC+NLP
Sbjct: 121 LKQSGIDLAKNTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240
           ++Q +FF LI++YFP VYDIKHLM+FCNS HGGLNKLAELLEVER+G+CHQAGSDSLLT+
Sbjct: 181 DSQTDFFKLINVYFPTVYDIKHLMKFCNSLHGGLNKLAELLEVERVGICHQAGSDSLLTS 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQ 272
           CTFRKL++ FF G   KY+GVLYGLGVE GQ
Sbjct: 241 CTFRKLKENFFVGPLHKYSGVLYGLGVENGQ 271

BLAST of CSPI02G23860 vs. TAIR 10
Match: AT1G80780.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 434.1 bits (1115), Expect = 8.3e-122
Identity = 198/271 (73.06%), Postives = 234/271 (86.35%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MS+  K +SI IREVWNDNL+EE  LIRD+VD F Y+AMDTEFPG+VVRPVG+FK+  +Y
Sbjct: 1   MSLFLKDDSIQIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRPVGTFKSNADY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           +Y TL+ NV+ LK+IQLGLTFS+E GNLPTCGTDK+CIWQFNFREF++  DI+A DSIEL
Sbjct: 61  HYETLKTNVNILKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180
           L++ GID  K   +GID  RF ELLMSSGIVLN+N+ WVTFHSGYDFGYLLKLLTC+NLP
Sbjct: 121 LKQSGIDLAKNTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240
           ++Q +FF LI++YFP VYDIKHLM+FCNS HGGLNKLAELLEVER+G+CHQAGSDSLLT+
Sbjct: 181 DSQTDFFKLINVYFPTVYDIKHLMKFCNSLHGGLNKLAELLEVERVGICHQAGSDSLLTS 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQ 272
           CTFRKL++ FF G   KY+GVLYGLGVE GQ
Sbjct: 241 CTFRKLKENFFVGPLHKYSGVLYGLGVENGQ 271

BLAST of CSPI02G23860 vs. TAIR 10
Match: AT5G10960.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 433.3 bits (1113), Expect = 1.4e-121
Identity = 203/268 (75.75%), Postives = 235/268 (87.69%), Query Frame = 0

Query: 6   KGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEYNYRTL 65
           K +SI IREVW+ NL EEFALIR+IVD+F+YIAMDTEFPGVV++PV +FK  N+ NYRTL
Sbjct: 6   KEDSIMIREVWDYNLVEEFALIREIVDKFSYIAMDTEFPGVVLKPVATFKYNNDLNYRTL 65

Query: 66  RENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIELLRECG 125
           +ENVD LKLIQ+GLTFSDENGNLPTCGTDKFCIWQFNFREFNI +DIYAS+SIELLR+CG
Sbjct: 66  KENVDLLKLIQVGLTFSDENGNLPTCGTDKFCIWQFNFREFNIGEDIYASESIELLRQCG 125

Query: 126 IDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLPETQAE 185
           IDF+K  E+GIDV RFGEL+MSSGIVLND I WVTFH GYDFGYL+KLLTC+ LP  QA+
Sbjct: 126 IDFKKNIEKGIDVVRFGELMMSSGIVLNDAISWVTFHGGYDFGYLVKLLTCKELPLKQAD 185

Query: 186 FFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTACTFRK 245
           FF L+++YFP VYDIKHLM FCN   GGLN+LAEL+ VER+G+CHQAGSDSLLT  +FRK
Sbjct: 186 FFKLLYVYFPTVYDIKHLMTFCNGLFGGLNRLAELMGVERVGICHQAGSDSLLTLGSFRK 245

Query: 246 LRDTFFNGSTQKYAGVLYGLGVETGQTT 274
           L++ +F GST+KY GVLYGLGVE G TT
Sbjct: 246 LKERYFPGSTEKYTGVLYGLGVEDGTTT 273

BLAST of CSPI02G23860 vs. TAIR 10
Match: AT1G80780.3 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 429.9 bits (1104), Expect = 1.6e-120
Identity = 196/268 (73.13%), Postives = 232/268 (86.57%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MS+  K +SI IREVWNDNL+EE  LIRD+VD F Y+AMDTEFPG+VVRPVG+FK+  +Y
Sbjct: 1   MSLFLKDDSIQIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRPVGTFKSNADY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           +Y TL+ NV+ LK+IQLGLTFS+E GNLPTCGTDK+CIWQFNFREF++  DI+A DSIEL
Sbjct: 61  HYETLKTNVNILKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180
           L++ GID  K   +GID  RF ELLMSSGIVLN+N+ WVTFHSGYDFGYLLKLLTC+NLP
Sbjct: 121 LKQSGIDLAKNTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240
           ++Q +FF LI++YFP VYDIKHLM+FCNS HGGLNKLAELLEVER+G+CHQAGSDSLLT+
Sbjct: 181 DSQTDFFKLINVYFPTVYDIKHLMKFCNSLHGGLNKLAELLEVERVGICHQAGSDSLLTS 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVE 269
           CTFRKL++ FF G   KY+GVLYGLGVE
Sbjct: 241 CTFRKLKENFFVGPLHKYSGVLYGLGVE 268

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SKZ23.7e-12274.63Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9SAI21.2e-12073.06Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9LEU42.0e-12075.75Probable CCR4-associated factor 1 homolog 10 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9S9P22.5e-9462.12Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q3ZC015.0e-7953.73CCR4-NOT transcription complex subunit 7 OS=Bos taurus OX=9913 GN=CNOT7 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LSM45.4e-161100.00Poly(A)-specific ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_2G382590 PE=3 SV... [more]
A0A5A7VC992.7e-16099.64Poly(A)-specific ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A1S3BD021.7e-15999.27Poly(A)-specific ribonuclease OS=Cucumis melo OX=3656 GN=LOC103488343 PE=3 SV=1[more]
A0A6J1KLE02.4e-14891.24Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111496296 PE=3 S... [more]
A0A6J1GJJ82.4e-14891.24Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111454439 PE=3... [more]
Match NameE-valueIdentityDescription
XP_004138706.11.1e-160100.00probable CCR4-associated factor 1 homolog 7 [Cucumis sativus] >KGN62986.1 hypoth... [more]
KAA0064894.15.5e-16099.64putative CCR4-associated factor 1-like protein 7 [Cucumis melo var. makuwa][more]
XP_008445257.13.6e-15999.27PREDICTED: LOW QUALITY PROTEIN: probable CCR4-associated factor 1 homolog 7 [Cuc... [more]
XP_038886120.13.1e-15596.35probable CCR4-associated factor 1 homolog 7 [Benincasa hispida][more]
XP_022951690.14.9e-14891.24probable CCR4-associated factor 1 homolog 7 [Cucurbita moschata] >XP_023002466.1... [more]
Match NameE-valueIdentityDescription
AT2G32070.12.6e-12374.63Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G80780.18.3e-12273.06Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G80780.28.3e-12273.06Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G10960.11.4e-12175.75Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G80780.31.6e-12073.13Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006941Ribonuclease CAF1PFAMPF04857CAF1coord: 162..243
e-value: 2.7E-6
score: 26.9
IPR006941Ribonuclease CAF1PFAMPF04857CAF1coord: 15..136
e-value: 1.9E-12
score: 47.1
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 2..268
e-value: 1.8E-100
score: 337.6
IPR039637CCR4-NOT transcription complex subunit 7/8/Pop2PANTHERPTHR10797CCR4-NOT TRANSCRIPTION COMPLEX SUBUNITcoord: 1..274
NoneNo IPR availablePANTHERPTHR10797:SF68CCR4-ASSOCIATED FACTOR 1 HOMOLOG 10-RELATEDcoord: 1..274
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 12..265

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G23860.1CSPI02G23860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043928 exonucleolytic catabolism of deadenylated mRNA
biological_process GO:0090503 RNA phosphodiester bond hydrolysis, exonucleolytic
cellular_component GO:0030015 CCR4-NOT core complex
cellular_component GO:0005634 nucleus
cellular_component GO:0000932 P-body
cellular_component GO:0030014 CCR4-NOT complex
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004535 poly(A)-specific ribonuclease activity