CSPI02G23000 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G23000
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionE3 ubiquitin-protein ligase
LocationChr2: 19920912 .. 19926288 (-)
RNA-Seq ExpressionCSPI02G23000
SyntenyCSPI02G23000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGAAGAGAGAGAGAGAGAGAGAAATGTGATCTATAAATACAAAAAGGAAAAAAAAAAGAAAAGAAGGAAGATTCTCAGGCGAAGGTAGGCAAAGGAAAGCAATGGAGGTCTAGAGCTGGCTGACTCTATCCCTACCTCCCTGATAAAGCAATTTCTCCGATTCACTTCAATTCTTATTCTTCTTCAAACGCCGTCCTCTCTCCGCTCCTCCGATTTCCTTTCCTCCCACCTCTCATCGGCGTTGGCTCAGTCCGGTGAGGTTCTCTGTCTTCGACGAGAATTCGCTTTTTGTTTCTTTTCTTTTCCTTTTTCAATTCGCGAAATGCTTTGTGTGTTTCTGTATCTTTCAATGTAGAACTCTACTTAGAAACGGTTTTTTGTTTTCTTTGTCGTGGGTTTAGGGTTTGAATTGATGGTTATTTTGGTTGGATCATTTTAATTTACAACAGGATCAGTCAACGTTGTGTAGTGAGGGGTTTTGATCCGTGTTCTTTGGGAAATTCAACTCGTAATTTTTTAATTCCGTTTGAAAGAATGATTGGAGCTGTGCATTTAGTCTTTGTTTGATTCCGTATGATTCTGAGAAACTTAAGGGAAATTCTGATACTACGGGACATAGGAAACCTCATTTCTTTAACTATCTTATCTTTCTTTTTTCTGAAAATCGTGTTAAACCTCTTAATTCTGGTTGAAAAAGAACTTTGCAGGTTTTTCATTAACGTCTCGAGTAATGCATATATCTTGAGAAAGAAAGGGGTCCTTTATATCGGTTATGTGATTTTTTTCCTTCGTTCCTTCGTTCCTTCGTTCCTTCATTCCTCCTTTTCTTTCTTTCTTTCTTTTTTTTTTTTTGTTGTGGTGGTCATGGTGTGGAGGGGGTTATATCGGTGGAAAACCAAAGCTGTGTTTGCATGATATCGTGGAGTGAAGCTGTTATTAACTTATCGCATGAAGTCTTTATTTGATCTTCCATTTGTTGTAGGTGTCTTAAACTTTATTTGGATGTTTCGATAGTAGTTGTGTATGAATCTATGATATGCCTAAAGAACTTAGATACCAGTTAAATTAAATGCAAGATTTACTACTTGTACCAGTATGGTATTTCATAATTGTGTTAAAACTATGGCTAAACTTCTGGTGTTCATATACTACTTGTACCAGTATGGTATTTCATATTTGTGTTAAAACTATGGCTAAACTTCTGGAGTTCATTTACTACTTCTTTAATATGATTATTGATATATATATTTTTTTCAGATTGGCAGCTTTGTGGATTGACAGAGTTGCATCAAAGGGCTGCTGCAACATCAATTTAAGTTATCTGTAAGTGATATATTTACACATGAGATTTTTTTCTTCTTCTTCTTTTTCCTTTTTTGGTCTAATGTTTTATGTCTCTACTTAACGTGTTTTGCAGTGGTTGGGTGAAATGCTTTGTTCAATTCCTTTGTTGTTTACGTTATAAATATATTATGTCTTGTTTCATTCTTTTGTTGTTTATGTTATAAATATATTATGTTTTGTTTCATTCTTTTGTTGTTTTTCCCCATGGAAGGTTTAAATAATGTTTGAGCTAAATTCAGCAAACTTATGTCCCTGTTTGGTGGATGCTATGGTTTTCCCTTGCTTAAAATTGTAAAAGGAAATTGTAAAAAAATGTTATGCTTGATAATAAATTTCAACATTTTTCTGTTTCTTGCGAGCATATGTAATTGGTCTGTCTGAATTTATTGTTGTAATTATTTTCTTTTTTAGCAGATTGAACTTTGTTACTTTTCCGTCACTTTGTGGTGTCCGCCAAATTGGTTTTCTGCTTACACTTTGGACTGGCCTGGTATATCAAATTGGTGTTGAATTTGTGAGTAGTTGCAAATCAGAAGTGGCATGTAGTGGGACAAACACGGCAGCCTTGTTTTAGAGTTCTATGCTTTCTTTTTCCTTCATTGTGTTGCTGAACTTAGATGGACGTTCCCTCAGTGGAACTAAATTGTGATAGTGAAGGAGACTCTTACCCTTTATTAATGGCACGACCTGAACATTCTAACAGCAGTGAGCATATCATTGACATAACTGGTGCTGGTGATTCTGGCCCCTCTGGTCTGCCACATGGTAGGAGTTCAAATGGCTTAAATTCATCGCAGCCTGAAGATAGACCTTCAAGTAGTACACGAGTTCCCTTGTCCCAACCCTCAACTTCTTCTACTGGATCAAATTCTAGAAATTCTTCATTCATAAGAAGAGGAGATGCACGACGTCGTAGAAGTCCATTAAATTCTGGCTTATGGATATCTATTGAATTACTACTCACTATGAGCCAGATTATTGCAGCGATTATTGTCTTATCATTGTCAAAGAATGAAAAACCCCGTGCCCCTTTGTTTGCATGGATTGTGGGATATGCATCCGGATGTGGTGCCACCCTTCCTCTTCTATACTGGCGTTATCGACACCGCAATCAGGCTTCAGAACAAGATTCTCTTCAGTCCAGCCAGAGTTCTTCTCGCATTAATGTTCCTGCTGGGCCATTCTCTCTTTCAGTTTCTAGGGCATCAGAAGGGGAAGAACTTCAGCATCCTGCCCCATCTCCTAGAGGCAGTCAAGGGTCAGGAGTACTATCAGCAAGGTGTGTGTTTTTTGTGCTTGTACCAGAATACTTTAATAAATGGTTGATAACTCATCCTTTTAGATCATTAAAGTTGAATTGGGAGAGAGATAGAAACATGTCCATGTAATAATTCTGGAAATTTTTTGTACAAGATTTCTTACTTCTTACTTCAATTTTCAAATTATGCATGAGATCCCAATTCCTCTGCATTATTACGTTTATGTAGATCAATCATCAGAGCAGTTCATCTAACCTCATAAGGGTGTAGCAATCTCATTAGGCAGGTTGCCATTTTGACTGAATCACTCATTCATCATTCACGTTTATTGTATTCTTTTTCCTTGAACAAAGAGACTAACGTATGCCAGAACTGCCCTGGCCTTGACCTTGATTAACACCAAATACCTCTAAAAATCATGCTCCAAGGTGTGGCAGTTTCTTGCTTACTGTGGTTGCTCAATTCCTTATTTCTTAATCTTTTCTTGATGTTTTTTTTTTGTTCTCTCAAAGTTGAACAATAATTGCCCTTACATTCGAGTCTTTTTGTAATTTTGCGTGCAGATTAAAAGTACTGGTTGAATATTTTAAGATGGGTCTGGATTGCTTCTTTGCTGTTTGGTTTGTAGTTGGTAATGTATGGATCTTTGGAGGGCATTCATCAGCCTCTGAGGCTCCTAACTTGTACAGGTTTGTGCTCTTAGACTATTTGTATTTGTAGAGGCATAGCGGCATTCCTTGAATGGATTTTATTAATTTCTTTCTTTCTTTGCAGGCTATGTATAGTGTTTCTTACCTTCAGCTGTATTGGTTATGCAATGCCTTTCATTCTATGTGTAACCATCTGCTGTTGTCTTCCTTGTATAATTTCCATCCTTGGGTTTAGAGAGGACTTGTCACAGACACGAGGAGCCACATCGGAATCTATTAATGCATTACCAACTTATAAGTTTAAACTGAAGAAAAGTAGAAGTGGGGATGACAGAGAGAACAACTCCGGGGCTGGTGAAGGAGGAGGGGTTGTGGCTGCTGGAACTGAAAAGGAGCGAGTGATTTCTGGAGAAGATGCTGTAAGTACAGCACACACTGTCCTATCGAATACTAATTTTATCACTGTTTACGTTAGTATCTGTTTCTTTGGTGTCATTTGATAACCATTCTGTTCTTTTTTACTTTATTTTAATTTTTTAATATTGATTACCATTCACTGTTTGGATTTCATTTTTTAAATTTGAAAAGCTCTTTTGAAATCGTTGGGGAAGCTTTTATTTATTTATTTAATATTATCAATGTTTTTAAAAAGTTTTTCAGAATTTCACATTTTCCCTCTCCAACCTATACCTTCTCTTCTCACTTTAAAATATTTCCCGCTCCTCCTCACTTAAAAGTATTTCCATCTCCTTATCATTTTTTTTCTTTCTAAGCTTTTTCTTAACTTTATCCCTATACAATTCTTCCCTTTCTCTTTAACTTATCTTTTCCCTCTTCATAGTCTCTTCTTCTCCCTCTAACTTATCTTTATCATCTTAGCATTTTCTTCTCATACTTCTTTCTCAAGAATTTTCCCTCCATAAGTTTCTTGATTTTTCCCTCTTTTAACTTTCTTTCCCACCTAAGATGACTTACTTGTATTTTGAAAAAAGAAAATAGAAAGGACACACATACTTTGCCTACTTTTTCTCGTAAACAAAAAACCAAAAATTGTGAGATTAAAAAACCATTTTAGGAGTGTTTTTAATTCAAGCCAAAATTTGGATTTTTTTTAATCTAAAAACTCGTTTTTGGAATTTGGCTAAGTATTGAAACTTAAACGGCTGGAAGGGTTATGGCCTGAGATTTCTCTATGAATCTGCCAGAAACAAAAATCCCACATTACAAATTAAATGGATTACTGGAAATTGTACGATGGAAGTTTGTTGTGTTTTTGTAAGTCATTTTCATTAAAAAGAAAAAGGAAGATATTTATTGATCGCGTTCTGGTTAATACTTCTCCATTTTACAAATGATTCCACGAATGTTTCAATCAGAGTCTAGTTTCTGGTGCATTCATTCTCATAGAATTGATTGACCAAATCTAATTGTTTTAAAAATTATGTGCAGGTCTGTTGCATCTGCCTAGCAAAATACGCCAACAATGATGAACTGAGAGAGTTGCCATGTTCACACTTTTTCCACAAGGACTGCGTAGATAAGTGGTTGAAGATCAATGCTCTGTGCCCGCTTTGCAAAGCCGAGGTTGGTGAGAGTATCGTCGGCTCTCTCGAGGGGACAAACAGACAACAGGGTGATTAGCGAGGGGAGTGGGCTGACTACATTGGCGCCATAAGTTCTCTGGATGGGTTCTGCTTGAATTCAAAATTTCACGGATACAAGTGTGTAGTATGTGCAAGCTCATCCTCTCTTGAGCTGCTAAACCTTATTTTTTGGGTTCGTATGCGGCATTCATTAGAGCCTTGAACAAGCTCCTCCTGTAGCAAATCCATCCATTGTCACCAATATAGGCAGAGTTATATGGTGCACGCGGGACGTTTTGAATGAGGAGAGATGGTTATGCTTTTGACCTTTTTGATAGAAAGTGAAAGTTGCTGATGTTGCAAAGGCTTATACTTGTATAATCACACTCCTTGTTCTTGTATTGCCCTCTTCAATTTTGTGGTGTAATGGCTATGATAACGAATAATCATCCGTAGAGCATACTATGCAATTCTAGTATAATATACATACATACATATATATATATATATTATATGTATATATGTTTGTAAGTTCACAC

mRNA sequence

AAAGAAGAGAGAGAGAGAGAGAGAAATGTGATCTATAAATACAAAAAGGAAAAAAAAAAGAAAAGAAGGAAGATTCTCAGGCGAAGGTAGGCAAAGGAAAGCAATGGAGGTCTAGAGCTGGCTGACTCTATCCCTACCTCCCTGATAAAGCAATTTCTCCGATTCACTTCAATTCTTATTCTTCTTCAAACGCCGTCCTCTCTCCGCTCCTCCGATTTCCTTTCCTCCCACCTCTCATCGGCGTTGGCTCAGTCCGATTGGCAGCTTTGTGGATTGACAGAGTTGCATCAAAGGGCTGCTGCAACATCAATTTAAGTTATCTATTGAACTTTGTTACTTTTCCGTCACTTTGTGGTGTCCGCCAAATTGGTTTTCTGCTTACACTTTGGACTGGCCTGGTATATCAAATTGGTGTTGAATTTGTGAGTAGTTGCAAATCAGAAGTGGCATGTAGTGGGACAAACACGGCAGCCTTGTTTTAGAGTTCTATGCTTTCTTTTTCCTTCATTGTGTTGCTGAACTTAGATGGACGTTCCCTCAGTGGAACTAAATTGTGATAGTGAAGGAGACTCTTACCCTTTATTAATGGCACGACCTGAACATTCTAACAGCAGTGAGCATATCATTGACATAACTGGTGCTGGTGATTCTGGCCCCTCTGGTCTGCCACATGGTAGGAGTTCAAATGGCTTAAATTCATCGCAGCCTGAAGATAGACCTTCAAGTAGTACACGAGTTCCCTTGTCCCAACCCTCAACTTCTTCTACTGGATCAAATTCTAGAAATTCTTCATTCATAAGAAGAGGAGATGCACGACGTCGTAGAAGTCCATTAAATTCTGGCTTATGGATATCTATTGAATTACTACTCACTATGAGCCAGATTATTGCAGCGATTATTGTCTTATCATTGTCAAAGAATGAAAAACCCCGTGCCCCTTTGTTTGCATGGATTGTGGGATATGCATCCGGATGTGGTGCCACCCTTCCTCTTCTATACTGGCGTTATCGACACCGCAATCAGGCTTCAGAACAAGATTCTCTTCAGTCCAGCCAGAGTTCTTCTCGCATTAATGTTCCTGCTGGGCCATTCTCTCTTTCAGTTTCTAGGGCATCAGAAGGGGAAGAACTTCAGCATCCTGCCCCATCTCCTAGAGGCAGTCAAGGGTCAGGAGTACTATCAGCAAGATTAAAAGTACTGGTTGAATATTTTAAGATGGGTCTGGATTGCTTCTTTGCTGTTTGGTTTGTAGTTGGTAATGTATGGATCTTTGGAGGGCATTCATCAGCCTCTGAGGCTCCTAACTTGTACAGGCTATGTATAGTGTTTCTTACCTTCAGCTGTATTGGTTATGCAATGCCTTTCATTCTATGTGTAACCATCTGCTGTTGTCTTCCTTGTATAATTTCCATCCTTGGGTTTAGAGAGGACTTGTCACAGACACGAGGAGCCACATCGGAATCTATTAATGCATTACCAACTTATAAGTTTAAACTGAAGAAAAGTAGAAGTGGGGATGACAGAGAGAACAACTCCGGGGCTGGTGAAGGAGGAGGGGTTGTGGCTGCTGGAACTGAAAAGGAGCGAGTGATTTCTGGAGAAGATGCTGTCTGTTGCATCTGCCTAGCAAAATACGCCAACAATGATGAACTGAGAGAGTTGCCATGTTCACACTTTTTCCACAAGGACTGCGTAGATAAGTGGTTGAAGATCAATGCTCTGTGCCCGCTTTGCAAAGCCGAGGTTGGTGAGAGTATCGTCGGCTCTCTCGAGGGGACAAACAGACAACAGGGTGATTAGCGAGGGGAGTGGGCTGACTACATTGGCGCCATAAGTTCTCTGGATGGGTTCTGCTTGAATTCAAAATTTCACGGATACAAGTGTGTAGTATGTGCAAGCTCATCCTCTCTTGAGCTGCTAAACCTTATTTTTTGGGTTCGTATGCGGCATTCATTAGAGCCTTGAACAAGCTCCTCCTGTAGCAAATCCATCCATTGTCACCAATATAGGCAGAGTTATATGGTGCACGCGGGACGTTTTGAATGAGGAGAGATGGTTATGCTTTTGACCTTTTTGATAGAAAGTGAAAGTTGCTGATGTTGCAAAGGCTTATACTTGTATAATCACACTCCTTGTTCTTGTATTGCCCTCTTCAATTTTGTGGTGTAATGGCTATGATAACGAATAATCATCCGTAGAGCATACTATGCAATTCTAGTATAATATACATACATACATATATATATATATATTATATGTATATATGTTTGTAAGTTCACAC

Coding sequence (CDS)

ATGGACGTTCCCTCAGTGGAACTAAATTGTGATAGTGAAGGAGACTCTTACCCTTTATTAATGGCACGACCTGAACATTCTAACAGCAGTGAGCATATCATTGACATAACTGGTGCTGGTGATTCTGGCCCCTCTGGTCTGCCACATGGTAGGAGTTCAAATGGCTTAAATTCATCGCAGCCTGAAGATAGACCTTCAAGTAGTACACGAGTTCCCTTGTCCCAACCCTCAACTTCTTCTACTGGATCAAATTCTAGAAATTCTTCATTCATAAGAAGAGGAGATGCACGACGTCGTAGAAGTCCATTAAATTCTGGCTTATGGATATCTATTGAATTACTACTCACTATGAGCCAGATTATTGCAGCGATTATTGTCTTATCATTGTCAAAGAATGAAAAACCCCGTGCCCCTTTGTTTGCATGGATTGTGGGATATGCATCCGGATGTGGTGCCACCCTTCCTCTTCTATACTGGCGTTATCGACACCGCAATCAGGCTTCAGAACAAGATTCTCTTCAGTCCAGCCAGAGTTCTTCTCGCATTAATGTTCCTGCTGGGCCATTCTCTCTTTCAGTTTCTAGGGCATCAGAAGGGGAAGAACTTCAGCATCCTGCCCCATCTCCTAGAGGCAGTCAAGGGTCAGGAGTACTATCAGCAAGATTAAAAGTACTGGTTGAATATTTTAAGATGGGTCTGGATTGCTTCTTTGCTGTTTGGTTTGTAGTTGGTAATGTATGGATCTTTGGAGGGCATTCATCAGCCTCTGAGGCTCCTAACTTGTACAGGCTATGTATAGTGTTTCTTACCTTCAGCTGTATTGGTTATGCAATGCCTTTCATTCTATGTGTAACCATCTGCTGTTGTCTTCCTTGTATAATTTCCATCCTTGGGTTTAGAGAGGACTTGTCACAGACACGAGGAGCCACATCGGAATCTATTAATGCATTACCAACTTATAAGTTTAAACTGAAGAAAAGTAGAAGTGGGGATGACAGAGAGAACAACTCCGGGGCTGGTGAAGGAGGAGGGGTTGTGGCTGCTGGAACTGAAAAGGAGCGAGTGATTTCTGGAGAAGATGCTGTCTGTTGCATCTGCCTAGCAAAATACGCCAACAATGATGAACTGAGAGAGTTGCCATGTTCACACTTTTTCCACAAGGACTGCGTAGATAAGTGGTTGAAGATCAATGCTCTGTGCCCGCTTTGCAAAGCCGAGGTTGGTGAGAGTATCGTCGGCTCTCTCGAGGGGACAAACAGACAACAGGGTGATTAG

Protein sequence

MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSGPSGLPHGRSSNGLNSSQPEDRPSSSTRVPLSQPSTSSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQIIAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSSRINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVWFVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFREDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGEDAVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGESIVGSLEGTNRQQGD*
Homology
BLAST of CSPI02G23000 vs. ExPASy Swiss-Prot
Match: Q8LDB8 (E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g63170 PE=2 SV=2)

HSP 1 Score: 169.9 bits (429), Expect = 6.4e-41
Identity = 112/331 (33.84%), Postives = 166/331 (50.15%), Query Frame = 0

Query: 109 ISIELLLTMSQIIAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQAS 168
           + ++ +  ++ ++ A  VL LS +E P  PL  WI+GY   C   +  +   YR RN   
Sbjct: 75  VVLDFVWNLAFVVVATAVLVLSSDENPNMPLRVWIIGYGLQCMMHMVCVCVEYRRRNSRR 134

Query: 169 EQD-----SLQSSQSSSRINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLK 228
            +D     S  SS SSS ++   G   L +SR S+   L+            G L     
Sbjct: 135 RRDLSPRSSSSSSSSSSSMDEEEG---LGLSRNSDERYLE-----------LGQLENENN 194

Query: 229 VLVEYFKMGLDCFFAVWFVVGNVWI-FGGHSSASEAPNLYRLCIVFLTFS------CIGY 288
              ++ +        +W+V+G  W+  GG   A  +P LY LCIVFL F       C+  
Sbjct: 195 SFAKHLESANTMISFIWWVIGFYWVSSGGQELAQGSPQLYWLCIVFLGFDVFFVVFCV-- 254

Query: 289 AMPFILCVTICCCLPCIISILGFREDLSQTRGATSESINALPTYKFKLKKSRSGDDRENN 348
           A+  ++ + +CCCLPCII++L     +++  GA+ E I+ L  +KF+          +  
Sbjct: 255 ALACVIGIAVCCCLPCIIAVL---YAVAEQEGASKEDIDQLTKFKFRKVGDTMKHTVDEE 314

Query: 349 SGAGEGGGVVA-AGTEK--ERVISGEDAVCCICLAKYANNDELRELPCSHFFHKDCVDKW 408
            G G+ GGV+   GT+   E  +  EDA CCICL+ Y +  ELRELPC H FH  CVDKW
Sbjct: 315 QGQGDSGGVMTECGTDSPVEHALPHEDAECCICLSAYEDETELRELPCGHHFHCGCVDKW 374

Query: 409 LKINALCPLCKAEVGESIVGSLEGTNRQQGD 425
           L INA CPLCK  +       L+ +N ++G+
Sbjct: 375 LYINATCPLCKYNI-------LKSSNYEEGE 379

BLAST of CSPI02G23000 vs. ExPASy Swiss-Prot
Match: Q9LN71 (E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g12760 PE=1 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 4.3e-37
Identity = 113/355 (31.83%), Postives = 172/355 (48.45%), Query Frame = 0

Query: 77  STSSTGSNSRNSSFIRRGDARRRRSPLNSGLW------ISIELLLTMSQIIAAIIVLSLS 136
           S +S+G   R  S + R  A  +     S  W      + ++++  ++ +  A  +L +S
Sbjct: 64  SRASSGRVMREPSMLVREAAAEQLEERQSD-WAYSKPVVVLDIVWNLAFVSVATAILVMS 123

Query: 137 KNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSSRINVPAGPFS 196
           + E P  PL  W++GYA  C   +  +   YR RN+          + ++R      P S
Sbjct: 124 RKEHPIMPLRVWLLGYALQCVLHMVCVCVEYRRRNR----------RRTNRTTTTTPPRS 183

Query: 197 LSVSRASEGEELQHPAPSPRGSQG-----SGVLSARLKVLVEYFKMGLDCFFAVWFVVGN 256
            S S +S    L+  A   R + G      G L      + ++ +     F  +W+++G 
Sbjct: 184 RSSSSSSSSSSLEEEALGSRRNSGVQDLSLGHLDTESSSVAKHLESANTMFSFIWWIIGF 243

Query: 257 VWI-FGGHSSASEAPNLYRLCIVFLTFS------CIGYAMPFILCVTICCCLPCIISILG 316
            W+  GG   A E+P +Y L IVFL F       C+  A+  ++ + +CCCLPCII++L 
Sbjct: 244 YWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCV--ALACVIGIAVCCCLPCIIAVL- 303

Query: 317 FREDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVA-AGTEK--ERV 376
               ++   GA+ E I  L   KFK +K    +   N+   G   G++   GT+   E  
Sbjct: 304 --YAVADQEGASKEDIEQLT--KFKFRKLGDANKHTNDEAQGTTEGIMTECGTDSPIEHT 363

Query: 377 ISGEDAVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGES 411
           +  EDA CCICL+ Y +  ELRELPC H FH  CVDKWL INA CPLCK  + +S
Sbjct: 364 LLQEDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINATCPLCKYNILKS 400

BLAST of CSPI02G23000 vs. ExPASy Swiss-Prot
Match: Q93Z92 (E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g11680 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.4e-32
Identity = 120/416 (28.85%), Postives = 196/416 (47.12%), Query Frame = 0

Query: 27  SNSSEHIIDITGAGDSGPSGLPHGRS-SNGLNSSQPEDRPSSSTRVPLSQPSTSSTGSNS 86
           S+SS    + T   DS       GRS S+G+  + P   P+ +  + + + S     S  
Sbjct: 2   SSSSSTTTNTTTESDSSSLPTHIGRSNSDGIIDTTPFLPPTVTRTISVDEESNPIHRSAR 61

Query: 87  RN-----SSFIRRGDARR-RRSPL-------------NSGLW------ISIELLLTMSQI 146
           R      + F+R   +RR  R P                  W      + +++L  ++ +
Sbjct: 62  RQGLREAARFLRHAGSRRMMREPSMLVRETAAEQLEERQSDWAYSKPVVFLDILWNLAFV 121

Query: 147 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSS 206
              + VL LS++EKP  PL  W+VGY   C   +  +   YR R +    +        S
Sbjct: 122 AIGVAVLILSRDEKPNMPLRVWVVGYGIQCWLHMACVCVEYRRRRRRRHPE----DGGGS 181

Query: 207 RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW 266
            +   +    +S+++  +  E  +PA                    ++ +     F  +W
Sbjct: 182 GLTNSSSQQYVSLAQLEDRGETSNPA--------------------KHLESANTMFSFIW 241

Query: 267 FVVGNVWI-FGGHSSASEAPNLYRLCIVFLTFS------CIGYAMPFILCVTICCCLPCI 326
           +++G  W+  GG + +S++P LY LCI+FL F       C+  A+  ++ + +CCCLPCI
Sbjct: 242 WIIGFYWVSAGGQTLSSDSPQLYWLCIIFLGFDVFFVVFCV--ALACVIGLAVCCCLPCI 301

Query: 327 ISILGFREDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEK- 386
           I+IL     ++   GA+   I+ +P ++F    +++G + E  SG    G +   GT+  
Sbjct: 302 IAIL---YAVADQEGASKNDIDQMPKFRF----TKTG-NVEKLSGKAR-GIMTECGTDSP 361

Query: 387 -ERVISGEDAVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEV 408
            ER +S EDA CCICL +Y +  ELRELPC+H FH  C+DKWL IN+ CPLCK  +
Sbjct: 362 IERSLSPEDAECCICLCEYEDGVELRELPCNHHFHCTCIDKWLHINSRCPLCKFNI 382

BLAST of CSPI02G23000 vs. ExPASy Swiss-Prot
Match: Q8GUU2 (E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana OX=3702 GN=RIE1 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 2.1e-28
Identity = 87/320 (27.19%), Postives = 148/320 (46.25%), Query Frame = 0

Query: 109 ISIELLLTMSQIIAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQAS 168
           ++ ++L   + ++A+ ++L  +  E+P  P+  WI  Y   C   + L++  Y  RN   
Sbjct: 78  VAADILWNAALVLASAVMLVGTVEERPNEPIRVWICVYGLQCLFHVVLVWSEYWRRNSTR 137

Query: 169 EQDSLQSSQSSSRINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEY 228
               L+S             +++      + ++        +  +        +  ++ +
Sbjct: 138 RARDLESYDHED--------YNIEYDYEQDSDDNSTTYSFVKRCES-------INTVISF 197

Query: 229 FKMGLDCFFAVWFVVGNVWIF-GGHSSASEAPNLYRLCIVFLT----FSCIGYAMPFILC 288
                     +W+++G  W+  GG     EAPNLY L ++FL     F+     +  ++ 
Sbjct: 198 ----------IWWIIGFYWVVEGGDKLLGEAPNLYWLSVIFLAIDVFFAVFCVVLACLVG 257

Query: 289 VTICCCLPCIISILGFREDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGG 348
           + +CCCLPCII++L     ++ T G +   +  LP YKFK   S    + +N +G G+  
Sbjct: 258 IALCCCLPCIIALL---YAVAGTEGVSEAELGVLPLYKFKAFHS----NEKNITGPGKMV 317

Query: 349 GVVAAG--TEKERVISGEDAVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCP 408
            +   G     ER +  EDA CCICL+ Y +  EL  LPC+H FH  C+ KWLK+ A CP
Sbjct: 318 PIPINGLCLATERTLLAEDADCCICLSSYEDGAELHALPCNHHFHSTCIVKWLKMRATCP 358

Query: 409 LCKAEVGESIVGSLEGTNRQ 422
           LCK  +       L+GT  Q
Sbjct: 378 LCKYNI-------LKGTTDQ 358

BLAST of CSPI02G23000 vs. ExPASy Swiss-Prot
Match: Q08CG8 (RING finger protein 44 OS=Danio rerio OX=7955 GN=rnf44 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 3.7e-12
Identity = 37/117 (31.62%), Postives = 53/117 (45.30%), Query Frame = 0

Query: 295 SILGFREDLSQT--RGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEK 354
           ++L   E L +   RG T   I  LP+Y+F L+  +S                       
Sbjct: 355 ALLNLAERLGEAKPRGLTKADIEQLPSYRFNLENHQS----------------------- 414

Query: 355 ERVISGEDAVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGE 410
                 E  +C +C + + +   LR LPC+H FH  CVDKWLK N  CP+C+A+  E
Sbjct: 415 ------EQTLCVVCFSDFESRQLLRVLPCNHEFHAKCVDKWLKTNRTCPICRADASE 442

BLAST of CSPI02G23000 vs. ExPASy TrEMBL
Match: A0A0A0LPE6 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G379250 PE=4 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 6.9e-240
Identity = 424/424 (100.00%), Postives = 424/424 (100.00%), Query Frame = 0

Query: 1   MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSGPSGLPHGRSSNGLNSSQ 60
           MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSGPSGLPHGRSSNGLNSSQ
Sbjct: 1   MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSGPSGLPHGRSSNGLNSSQ 60

Query: 61  PEDRPSSSTRVPLSQPSTSSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120
           PEDRPSSSTRVPLSQPSTSSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDRPSSSTRVPLSQPSTSSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSS 180
           IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSS
Sbjct: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSS 180

Query: 181 RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW 240
           RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW
Sbjct: 181 RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED 360
           EDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED
Sbjct: 301 EDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED 360

Query: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGESIVGSLEGTNR 420
           AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGESIVGSLEGTNR
Sbjct: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGESIVGSLEGTNR 420

Query: 421 QQGD 425
           QQGD
Sbjct: 421 QQGD 424

BLAST of CSPI02G23000 vs. ExPASy TrEMBL
Match: A0A1S3BBX9 (E3 ubiquitin-protein ligase At1g63170 OS=Cucumis melo OX=3656 GN=LOC103488256 PE=4 SV=1)

HSP 1 Score: 814.3 bits (2102), Expect = 2.4e-232
Identity = 411/424 (96.93%), Postives = 415/424 (97.88%), Query Frame = 0

Query: 1   MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSGPSGLPHGRSSNGLNSSQ 60
           MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDS  S LP GRSSNGLNS Q
Sbjct: 1   MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSASSSLPRGRSSNGLNSLQ 60

Query: 61  PEDRPSSSTRVPLSQPSTSSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120
           PEDRPSS+TRVPLSQP  SSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDRPSSNTRVPLSQPPISSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSS 180
           IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQ+SS
Sbjct: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQNSS 180

Query: 181 RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW 240
           RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW
Sbjct: 181 RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED 360
           EDL+QTRGATSESINALPTYKFKLKKSRSGDDRENNSG GEGGGVVAAGTEKERVISGED
Sbjct: 301 EDLTQTRGATSESINALPTYKFKLKKSRSGDDRENNSGTGEGGGVVAAGTEKERVISGED 360

Query: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGESIVGSLEGTNR 420
           AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGESI+ SLEGTNR
Sbjct: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGESIISSLEGTNR 420

Query: 421 QQGD 425
           QQGD
Sbjct: 421 QQGD 424

BLAST of CSPI02G23000 vs. ExPASy TrEMBL
Match: A0A6J1EPN3 (E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita moschata OX=3662 GN=LOC111436240 PE=4 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 8.2e-225
Identity = 394/424 (92.92%), Postives = 408/424 (96.23%), Query Frame = 0

Query: 1   MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSGPSGLPHGRSSNGLNSSQ 60
           MDVPSVELNC+SEGDSYPLLMA+PE+SNSSEHIID+TG GDS PS LPHGR+SNGLN SQ
Sbjct: 1   MDVPSVELNCESEGDSYPLLMAQPENSNSSEHIIDVTGTGDSTPSSLPHGRNSNGLNLSQ 60

Query: 61  PEDRPSSSTRVPLSQPSTSSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120
           PEDRPSSST+VPLSQPS SSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDRPSSSTQVPLSQPSISSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSS 180
           IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRH NQASEQ SLQSSQ+SS
Sbjct: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHHNQASEQGSLQSSQNSS 180

Query: 181 RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW 240
           RIN PAGPFSLSVSRASEGEELQHPAPSPRGSQGSGV++ R KVLVEY KMGLDCFFAVW
Sbjct: 181 RINAPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVVTGRFKVLVEYLKMGLDCFFAVW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED 360
           E+ +QTRGAT+ESINALP YKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED
Sbjct: 301 EEFTQTRGATAESINALPAYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED 360

Query: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGESIVGSLEGTNR 420
           AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCK EVGESI+ SL+G NR
Sbjct: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKTEVGESIISSLDGANR 420

Query: 421 QQGD 425
           QQGD
Sbjct: 421 QQGD 424

BLAST of CSPI02G23000 vs. ExPASy TrEMBL
Match: A0A5A7VGF8 (E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002960 PE=4 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 1.7e-222
Identity = 395/406 (97.29%), Postives = 398/406 (98.03%), Query Frame = 0

Query: 1   MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSGPSGLPHGRSSNGLNSSQ 60
           MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDS  S LP GRSSNGLNS Q
Sbjct: 1   MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSASSSLPRGRSSNGLNSLQ 60

Query: 61  PEDRPSSSTRVPLSQPSTSSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120
           PEDRPSS+TRVPLSQP  SSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDRPSSNTRVPLSQPPISSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSS 180
           IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQ+SS
Sbjct: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQNSS 180

Query: 181 RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW 240
           RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW
Sbjct: 181 RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED 360
           EDL+QTRGATSESINALPTYKFKLKKSRSGDDRENNSG GEGGGVVAAGTEKERVISGED
Sbjct: 301 EDLTQTRGATSESINALPTYKFKLKKSRSGDDRENNSGTGEGGGVVAAGTEKERVISGED 360

Query: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAE 407
           AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAE
Sbjct: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAE 406

BLAST of CSPI02G23000 vs. ExPASy TrEMBL
Match: A0A6J1KBA5 (E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita maxima OX=3661 GN=LOC111492338 PE=4 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 6.5e-222
Identity = 390/424 (91.98%), Postives = 405/424 (95.52%), Query Frame = 0

Query: 1   MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSGPSGLPHGRSSNGLNSSQ 60
           MDVPSVELNC+SEGDSYPLLMA+PE+SNSSEHIID+TG GDS PS LPHGR+SNGLN SQ
Sbjct: 1   MDVPSVELNCESEGDSYPLLMAQPENSNSSEHIIDVTGTGDSTPSSLPHGRNSNGLNLSQ 60

Query: 61  PEDRPSSSTRVPLSQPSTSSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120
           PED PSSST+VP SQPS SST SNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDSPSSSTQVPSSQPSISSTESNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSS 180
           IAAIIVLSLSKNEKPRAPLFAWIVGYA GCGATLPLLYWRYRH NQASEQ SLQSSQ+SS
Sbjct: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYAFGCGATLPLLYWRYRHHNQASEQGSLQSSQNSS 180

Query: 181 RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW 240
           RIN PAGPFSLSVSRASEGEELQHPAPSPRGSQGSGV++ R K+LVEY KMGLDCFFAVW
Sbjct: 181 RINAPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVVTGRFKMLVEYLKMGLDCFFAVW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED 360
           E+L+QTRGAT+ESINALP YKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED
Sbjct: 301 EELTQTRGATAESINALPAYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED 360

Query: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGESIVGSLEGTNR 420
           AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCK EVGESI+ SL+G NR
Sbjct: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKTEVGESIISSLDGANR 420

Query: 421 QQGD 425
           QQGD
Sbjct: 421 QQGD 424

BLAST of CSPI02G23000 vs. NCBI nr
Match: XP_004138872.1 (E3 ubiquitin-protein ligase At1g63170 [Cucumis sativus] >XP_011649787.1 E3 ubiquitin-protein ligase At1g63170 [Cucumis sativus] >XP_031736373.1 E3 ubiquitin-protein ligase At1g63170 [Cucumis sativus] >XP_031736374.1 E3 ubiquitin-protein ligase At1g63170 [Cucumis sativus])

HSP 1 Score: 839.3 bits (2167), Expect = 1.4e-239
Identity = 424/424 (100.00%), Postives = 424/424 (100.00%), Query Frame = 0

Query: 1   MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSGPSGLPHGRSSNGLNSSQ 60
           MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSGPSGLPHGRSSNGLNSSQ
Sbjct: 1   MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSGPSGLPHGRSSNGLNSSQ 60

Query: 61  PEDRPSSSTRVPLSQPSTSSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120
           PEDRPSSSTRVPLSQPSTSSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDRPSSSTRVPLSQPSTSSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSS 180
           IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSS
Sbjct: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSS 180

Query: 181 RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW 240
           RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW
Sbjct: 181 RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED 360
           EDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED
Sbjct: 301 EDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED 360

Query: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGESIVGSLEGTNR 420
           AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGESIVGSLEGTNR
Sbjct: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGESIVGSLEGTNR 420

Query: 421 QQGD 425
           QQGD
Sbjct: 421 QQGD 424

BLAST of CSPI02G23000 vs. NCBI nr
Match: XP_008445126.1 (PREDICTED: E3 ubiquitin-protein ligase At1g63170 [Cucumis melo] >XP_008445127.1 PREDICTED: E3 ubiquitin-protein ligase At1g63170 [Cucumis melo])

HSP 1 Score: 814.3 bits (2102), Expect = 4.9e-232
Identity = 411/424 (96.93%), Postives = 415/424 (97.88%), Query Frame = 0

Query: 1   MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSGPSGLPHGRSSNGLNSSQ 60
           MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDS  S LP GRSSNGLNS Q
Sbjct: 1   MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSASSSLPRGRSSNGLNSLQ 60

Query: 61  PEDRPSSSTRVPLSQPSTSSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120
           PEDRPSS+TRVPLSQP  SSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDRPSSNTRVPLSQPPISSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSS 180
           IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQ+SS
Sbjct: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQNSS 180

Query: 181 RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW 240
           RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW
Sbjct: 181 RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED 360
           EDL+QTRGATSESINALPTYKFKLKKSRSGDDRENNSG GEGGGVVAAGTEKERVISGED
Sbjct: 301 EDLTQTRGATSESINALPTYKFKLKKSRSGDDRENNSGTGEGGGVVAAGTEKERVISGED 360

Query: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGESIVGSLEGTNR 420
           AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGESI+ SLEGTNR
Sbjct: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGESIISSLEGTNR 420

Query: 421 QQGD 425
           QQGD
Sbjct: 421 QQGD 424

BLAST of CSPI02G23000 vs. NCBI nr
Match: XP_038884345.1 (E3 ubiquitin-protein ligase At1g63170 [Benincasa hispida])

HSP 1 Score: 808.5 bits (2087), Expect = 2.7e-230
Identity = 408/422 (96.68%), Postives = 414/422 (98.10%), Query Frame = 0

Query: 1   MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSGPSGLPHGRSSNGLNSSQ 60
           MDVPSVELN +SEGDSYPLLMARPE+SNSSEHIIDITG GDS PSGLPHGRSSNGLNS Q
Sbjct: 1   MDVPSVELNRESEGDSYPLLMARPENSNSSEHIIDITGTGDSAPSGLPHGRSSNGLNSLQ 60

Query: 61  PEDRPSSSTRVPLSQPSTSSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120
           PEDRPSSSTRVPLSQPS SSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDRPSSSTRVPLSQPSISSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSS 180
           IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQ+SS
Sbjct: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQNSS 180

Query: 181 RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW 240
           RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW
Sbjct: 181 RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED 360
           EDL+QTRGATSESINALPTYKFKLKKSR GDDRENNSGAGEGGGVVAAGTEKERVISGED
Sbjct: 301 EDLTQTRGATSESINALPTYKFKLKKSRCGDDRENNSGAGEGGGVVAAGTEKERVISGED 360

Query: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGESIVGSLEGTNR 420
           AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCK EVGES++ SLEGTNR
Sbjct: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKTEVGESLISSLEGTNR 420

Query: 421 QQ 423
           QQ
Sbjct: 421 QQ 422

BLAST of CSPI02G23000 vs. NCBI nr
Match: XP_023545842.1 (E3 ubiquitin-protein ligase At1g63170-like [Cucurbita pepo subsp. pepo] >XP_023545843.1 E3 ubiquitin-protein ligase At1g63170-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 791.2 bits (2042), Expect = 4.5e-225
Identity = 395/424 (93.16%), Postives = 409/424 (96.46%), Query Frame = 0

Query: 1   MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSGPSGLPHGRSSNGLNSSQ 60
           MDVPSVELNC+SEGDSYPLLMA+PE+SNSSEHIID+TG GDS PS LPHGR+SNGLN SQ
Sbjct: 1   MDVPSVELNCESEGDSYPLLMAQPENSNSSEHIIDVTGTGDSTPSSLPHGRNSNGLNLSQ 60

Query: 61  PEDRPSSSTRVPLSQPSTSSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120
           PEDRPSSST+VPLSQPS SSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDRPSSSTQVPLSQPSISSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSS 180
           IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRH NQASEQ SLQSSQ+SS
Sbjct: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHHNQASEQSSLQSSQNSS 180

Query: 181 RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW 240
           RIN PAGPFSLSVSRASEGEELQHPAPSPRGSQGSGV++ R KVLVEY KMGLDCFFAVW
Sbjct: 181 RINAPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVVTGRFKVLVEYLKMGLDCFFAVW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED 360
           E+L+QTRGAT+ESINALP YKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED
Sbjct: 301 EELTQTRGATAESINALPAYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED 360

Query: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGESIVGSLEGTNR 420
           AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCK EVGESI+ SL+G NR
Sbjct: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKTEVGESIISSLDGANR 420

Query: 421 QQGD 425
           QQGD
Sbjct: 421 QQGD 424

BLAST of CSPI02G23000 vs. NCBI nr
Match: XP_022929744.1 (E3 ubiquitin-protein ligase At1g63170-like [Cucurbita moschata] >XP_022929745.1 E3 ubiquitin-protein ligase At1g63170-like [Cucurbita moschata])

HSP 1 Score: 789.3 bits (2037), Expect = 1.7e-224
Identity = 394/424 (92.92%), Postives = 408/424 (96.23%), Query Frame = 0

Query: 1   MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSGPSGLPHGRSSNGLNSSQ 60
           MDVPSVELNC+SEGDSYPLLMA+PE+SNSSEHIID+TG GDS PS LPHGR+SNGLN SQ
Sbjct: 1   MDVPSVELNCESEGDSYPLLMAQPENSNSSEHIIDVTGTGDSTPSSLPHGRNSNGLNLSQ 60

Query: 61  PEDRPSSSTRVPLSQPSTSSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120
           PEDRPSSST+VPLSQPS SSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDRPSSSTQVPLSQPSISSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSS 180
           IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRH NQASEQ SLQSSQ+SS
Sbjct: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHHNQASEQGSLQSSQNSS 180

Query: 181 RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW 240
           RIN PAGPFSLSVSRASEGEELQHPAPSPRGSQGSGV++ R KVLVEY KMGLDCFFAVW
Sbjct: 181 RINAPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVVTGRFKVLVEYLKMGLDCFFAVW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED 360
           E+ +QTRGAT+ESINALP YKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED
Sbjct: 301 EEFTQTRGATAESINALPAYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGED 360

Query: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGESIVGSLEGTNR 420
           AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCK EVGESI+ SL+G NR
Sbjct: 361 AVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKTEVGESIISSLDGANR 420

Query: 421 QQGD 425
           QQGD
Sbjct: 421 QQGD 424

BLAST of CSPI02G23000 vs. TAIR 10
Match: AT4G32600.1 (RING/U-box superfamily protein )

HSP 1 Score: 503.8 bits (1296), Expect = 1.3e-142
Identity = 277/403 (68.73%), Postives = 310/403 (76.92%), Query Frame = 0

Query: 12  SEGDSYPLLMARPEHSNSS--EH-IIDITGAGDSGPSGLPHGRSSNGLNSSQ-PEDRPSS 71
           ++ D++   M R   +N S  EH IIDI        S   H R SN L   Q  E+RPS+
Sbjct: 14  NQADTFSRFMERSPENNLSRNEHIIIDIPRNAAIASSSSSHDRISNVLEPLQHEEERPST 73

Query: 72  STRVPLSQPSTSSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQIIAAIIVL 131
            + +   QP+T++  S+S   S  R    RRRRSPLNSGLWISIEL LT+ QIIAAI+VL
Sbjct: 74  VSPMAAPQPATATASSSSSMRSNPR--SVRRRRSPLNSGLWISIELFLTVGQIIAAIVVL 133

Query: 132 SLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSSRINVPAG 191
           SLSK+E PRAPLF WIVGYA GC ATLPLLYWRY H NQASEQD   S Q    +NV AG
Sbjct: 134 SLSKHEHPRAPLFTWIVGYACGCVATLPLLYWRYYHSNQASEQD---SGQHRPNLNVAAG 193

Query: 192 PFSLSVSRASEGEELQHPAPSPRGSQGSGVLS-ARLKVLVEYFKMGLDCFFAVWFVVGNV 251
           PF+ S+SR SE +  Q    S RGS+  G +S ARLKV+VEYFKM LDCFFAVWFVVGNV
Sbjct: 194 PFAFSISRTSEADGRQTNTTSSRGSRYPGFISAARLKVIVEYFKMALDCFFAVWFVVGNV 253

Query: 252 WIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFREDLSQT 311
           WIFGGHSSA+EAPNLYRLC+VFLTFSCIGYAMPFILC TICCCLPCIISILG+REDL+Q 
Sbjct: 254 WIFGGHSSAAEAPNLYRLCLVFLTFSCIGYAMPFILCTTICCCLPCIISILGYREDLTQP 313

Query: 312 RGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGEDAVCCIC 371
           RGAT ESINALPT+KFKLKKSRS  D +N S   E GGVVAAGT+ ER ISGEDAVCCIC
Sbjct: 314 RGATPESINALPTHKFKLKKSRSNGD-DNGSSTSE-GGVVAAGTDNERAISGEDAVCCIC 373

Query: 372 LAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVGE 410
           LAKYANN+ELRELPCSHFFHK+CVDKWLKINA CPLCK+EVGE
Sbjct: 374 LAKYANNEELRELPCSHFFHKECVDKWLKINASCPLCKSEVGE 409

BLAST of CSPI02G23000 vs. TAIR 10
Match: AT1G80400.1 (RING/U-box superfamily protein )

HSP 1 Score: 414.8 bits (1065), Expect = 8.1e-116
Identity = 231/422 (54.74%), Postives = 297/422 (70.38%), Query Frame = 0

Query: 1   MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSGPSG-------LPHGRSS 60
           MDV S+  N  +  D  PLLM   +H N  EHI+DIT   +   S         P G S 
Sbjct: 1   MDVSSIVRN-TTLTDQDPLLM---DHGN-REHIVDITSNDEEDSSSRSSLDELTPQGNSH 60

Query: 61  NG----LNSSQPEDRPSSSTRVPLSQPSTSSTGSNSRNSSFIRRGDARRRRSPLNSGLWI 120
            G    L+SS    +  +    P  +P++S  G N         G+ RR RSPLNSGLWI
Sbjct: 61  QGEETVLSSSNATQQHPTHPSPPQQRPTSSRRGDN---------GNGRRTRSPLNSGLWI 120

Query: 121 SIELLLTMSQIIAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASE 180
           S+EL++T++QI+AAI+V+ L+K+E P APLF W++GY  GC ATLP+LYWR+R  N+A+ 
Sbjct: 121 SVELVVTVAQIVAAIVVMVLAKDEHPEAPLFTWVIGYTCGCLATLPILYWRFRTYNRATG 180

Query: 181 QDSLQSSQSSSRINVPAGPF-SLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEY 240
           QDS Q + +SS+ N  + P+ ++SV++A++ E     + +PR +Q    L  RL  LV++
Sbjct: 181 QDSSQRA-TSSQGNSESTPYTAVSVAQAADEENSTGVSAAPRNNQVGESLRTRLNGLVDH 240

Query: 241 FKMGLDCFFAVWFVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICC 300
           FKM +DCFFAVWFVVGNVWIFGGHSS S++P LYRLCI FLTFSCIGYAMPFILC TICC
Sbjct: 241 FKMAIDCFFAVWFVVGNVWIFGGHSSPSDSPKLYRLCIAFLTFSCIGYAMPFILCATICC 300

Query: 301 CLPCIISILGFREDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAA 360
           CLPC+IS+LGFRE+ SQTRGAT+E+INALP Y+F   KS+S +D E  S  GE GG +  
Sbjct: 301 CLPCLISVLGFRENFSQTRGATAEAINALPVYRF---KSKSRNDLE-FSEEGE-GGFLLL 360

Query: 361 GTEKERVISGEDAVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKINALCPLCKAEVG 411
           G++K+R+ISGEDA CCICL +Y +++++RELPCSH FH DCVDKWLKINA CPLCK EVG
Sbjct: 361 GSQKKRLISGEDASCCICLTRYGDDEQVRELPCSHVFHVDCVDKWLKINATCPLCKNEVG 402

BLAST of CSPI02G23000 vs. TAIR 10
Match: AT4G26580.1 (RING/U-box superfamily protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.6e-42
Identity = 125/371 (33.69%), Postives = 183/371 (49.33%), Query Frame = 0

Query: 52  SSNGLNSSQPEDRPSSSTRVPLSQPSTS------STGSNSRNSSFIRR----GDARRR-- 111
           SS+   S+Q +D   ++   P S PS+S         S +R   F+RR     +A R   
Sbjct: 21  SSSSFASTQLDDNTHTA---PESSPSSSFFMRLAMRVSRARWFIFLRRVFHYQNASRSDL 80

Query: 112 -RSPLNSGLWISIELLLTMSQIIAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLY 171
             +P NS  W+  EL+  + QI      L+LSKNE+P  P+  WI GY  GC   L LLY
Sbjct: 81  GTNPFNSIAWMVSELIALLLQITVITSTLALSKNERPVWPMRLWITGYNVGCLLNLMLLY 140

Query: 172 WRYRHRNQASEQDSLQSSQSSSRINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVL 231
            RYR ++ + E                        +  S G+  Q         Q S   
Sbjct: 141 GRYRQQDTSHE------------------------NAFSFGDIEQ--------QQRSREE 200

Query: 232 SARLKVLVEYFKMGLDCFFAVWFVVGNVWIFGGH-SSASEAPNLYRLCIVFLTFSCIGYA 291
           + R   L+   +  L+ FFA+WFV+GNVW+F     S   AP L+ LCI  L ++ + Y+
Sbjct: 201 TTRCSHLMNKCRTSLELFFAIWFVIGNVWVFDSRFGSFHYAPILHVLCISLLAWNALCYS 260

Query: 292 MPFILCVTICCCLPCIISILGFREDL-SQTRGATSESINALPTYKFKLKKSRSGDDRENN 351
            PF+L + +CC +P + S LG+  ++ S  +GA+ + I++LP++K+KL    S   + NN
Sbjct: 261 FPFLLFLLLCCVVPLVSSFLGYNMNVGSSEKGASDDQISSLPSWKYKLIDETSDSSQANN 320

Query: 352 SGAGEGGGVVAAGTEKERVISGEDAVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKI 408
                                  D  CCICLAKY   +E+R+LPCSH FH  CVD+WL+I
Sbjct: 321 -----------------------DPECCICLAKYKEKEEVRKLPCSHRFHLKCVDQWLRI 333

BLAST of CSPI02G23000 vs. TAIR 10
Match: AT4G26580.2 (RING/U-box superfamily protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.6e-42
Identity = 125/371 (33.69%), Postives = 183/371 (49.33%), Query Frame = 0

Query: 52  SSNGLNSSQPEDRPSSSTRVPLSQPSTS------STGSNSRNSSFIRR----GDARRR-- 111
           SS+   S+Q +D   ++   P S PS+S         S +R   F+RR     +A R   
Sbjct: 21  SSSSFASTQLDDNTHTA---PESSPSSSFFMRLAMRVSRARWFIFLRRVFHYQNASRSDL 80

Query: 112 -RSPLNSGLWISIELLLTMSQIIAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLY 171
             +P NS  W+  EL+  + QI      L+LSKNE+P  P+  WI GY  GC   L LLY
Sbjct: 81  GTNPFNSIAWMVSELIALLLQITVITSTLALSKNERPVWPMRLWITGYNVGCLLNLMLLY 140

Query: 172 WRYRHRNQASEQDSLQSSQSSSRINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVL 231
            RYR ++ + E                        +  S G+  Q         Q S   
Sbjct: 141 GRYRQQDTSHE------------------------NAFSFGDIEQ--------QQRSREE 200

Query: 232 SARLKVLVEYFKMGLDCFFAVWFVVGNVWIFGGH-SSASEAPNLYRLCIVFLTFSCIGYA 291
           + R   L+   +  L+ FFA+WFV+GNVW+F     S   AP L+ LCI  L ++ + Y+
Sbjct: 201 TTRCSHLMNKCRTSLELFFAIWFVIGNVWVFDSRFGSFHYAPILHVLCISLLAWNALCYS 260

Query: 292 MPFILCVTICCCLPCIISILGFREDL-SQTRGATSESINALPTYKFKLKKSRSGDDRENN 351
            PF+L + +CC +P + S LG+  ++ S  +GA+ + I++LP++K+KL    S   + NN
Sbjct: 261 FPFLLFLLLCCVVPLVSSFLGYNMNVGSSEKGASDDQISSLPSWKYKLIDETSDSSQANN 320

Query: 352 SGAGEGGGVVAAGTEKERVISGEDAVCCICLAKYANNDELRELPCSHFFHKDCVDKWLKI 408
                                  D  CCICLAKY   +E+R+LPCSH FH  CVD+WL+I
Sbjct: 321 -----------------------DPECCICLAKYKEKEEVRKLPCSHRFHLKCVDQWLRI 333

BLAST of CSPI02G23000 vs. TAIR 10
Match: AT5G55970.1 (RING/U-box superfamily protein )

HSP 1 Score: 171.0 bits (432), Expect = 2.0e-42
Identity = 126/394 (31.98%), Postives = 195/394 (49.49%), Query Frame = 0

Query: 23  RPEHSNSSEHIIDITGAGDSGPSGLPHGRSSNGLNSSQPEDRPSSSTRVPLSQPSTSSTG 82
           +PE S+S+   I IT +  +  S  P G +S+ + ++  ++R  SS  + L+        
Sbjct: 8   QPELSSSN---ITITISSSALLSSSPRGDNSH-VAAANGQERSPSSFYIRLAMKV----- 67

Query: 83  SNSRNSSFIRR-------GDARRRRSPLNSGLWISIELLLTMSQIIAAIIVLSLSKNEKP 142
           S +R   F+RR         +    +P NS  W+  EL+  + Q+      L++SK E+P
Sbjct: 68  SRARWFIFLRRVFHYQNGSRSDLGSNPFNSSTWMMSELIALLVQLTVITFTLAISKEERP 127

Query: 143 RAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSSRINVPAGPFSLSVSR 202
             P+  WI GY  GC   L LLY RYR  +                IN   G     V +
Sbjct: 128 IWPVRLWITGYDVGCLLNLMLLYGRYRQLD----------------INQGNGFVLGDVEQ 187

Query: 203 ASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVWFVVGNVWIFGGH-SS 262
              G E                   R   L+   +  L+ FFA+WFV+GNVW+F     S
Sbjct: 188 QQRGRE-----------------ETRSSHLMNKCRTSLELFFAIWFVIGNVWVFDSRFGS 247

Query: 263 ASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFREDL-SQTRGATSES 322
              AP L+ LC+  L ++ I Y+ PF+L + +CC +P I S+LG+  ++ S  R A+ + 
Sbjct: 248 FHHAPKLHVLCVSLLAWNAICYSFPFLLFLFLCCLVPLISSLLGYNMNMGSSDRAASDDQ 307

Query: 323 INALPTYKFKLKKSRSGDDRENNSGAGEGGGVVAAGTEKERVISGEDAVCCICLAKYANN 382
           I++LP++KFK         R ++S         A+ ++ +     +D  CCICLAKY + 
Sbjct: 308 ISSLPSWKFK---------RIDDS---------ASDSDSDSATVTDDPECCICLAKYKDK 341

Query: 383 DELRELPCSHFFHKDCVDKWLKINALCPLCKAEV 408
           +E+R+LPCSH FH  CVD+WL+I + CPLCK ++
Sbjct: 368 EEVRKLPCSHKFHSKCVDQWLRIISCCPLCKQDL 341

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LDB86.4e-4133.84E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g631... [more]
Q9LN714.3e-3731.83E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g127... [more]
Q93Z922.4e-3228.85E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g116... [more]
Q8GUU22.1e-2827.19E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana OX=3702 GN=RIE1 PE=2 SV... [more]
Q08CG83.7e-1231.62RING finger protein 44 OS=Danio rerio OX=7955 GN=rnf44 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LPE66.9e-240100.00RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G379250 P... [more]
A0A1S3BBX92.4e-23296.93E3 ubiquitin-protein ligase At1g63170 OS=Cucumis melo OX=3656 GN=LOC103488256 PE... [more]
A0A6J1EPN38.2e-22592.92E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A5A7VGF81.7e-22297.29E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A6J1KBA56.5e-22291.98E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
Match NameE-valueIdentityDescription
XP_004138872.11.4e-239100.00E3 ubiquitin-protein ligase At1g63170 [Cucumis sativus] >XP_011649787.1 E3 ubiqu... [more]
XP_008445126.14.9e-23296.93PREDICTED: E3 ubiquitin-protein ligase At1g63170 [Cucumis melo] >XP_008445127.1 ... [more]
XP_038884345.12.7e-23096.68E3 ubiquitin-protein ligase At1g63170 [Benincasa hispida][more]
XP_023545842.14.5e-22593.16E3 ubiquitin-protein ligase At1g63170-like [Cucurbita pepo subsp. pepo] >XP_0235... [more]
XP_022929744.11.7e-22492.92E3 ubiquitin-protein ligase At1g63170-like [Cucurbita moschata] >XP_022929745.1 ... [more]
Match NameE-valueIdentityDescription
AT4G32600.11.3e-14268.73RING/U-box superfamily protein [more]
AT1G80400.18.1e-11654.74RING/U-box superfamily protein [more]
AT4G26580.11.6e-4233.69RING/U-box superfamily protein [more]
AT4G26580.21.6e-4233.69RING/U-box superfamily protein [more]
AT5G55970.12.0e-4231.98RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 363..403
e-value: 5.4E-7
score: 39.2
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 363..404
e-value: 1.5E-12
score: 47.6
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 363..404
score: 12.326995
IPR011016Zinc finger, RING-CH-typeSMARTSM00744ringv_2coord: 362..404
e-value: 0.0075
score: 8.2
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 346..411
e-value: 5.6E-19
score: 69.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 47..90
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..97
NoneNo IPR availablePANTHERPTHR46225:SF2C3H4 TYPE ZINC FINGER PROTEINcoord: 1..421
NoneNo IPR availablePANTHERPTHR46225C3H4 TYPE ZINC FINGER PROTEINcoord: 1..421
NoneNo IPR availableCDDcd16474RING-H2_RNF111_likecoord: 362..404
e-value: 8.37562E-18
score: 74.6553
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 355..409

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G23000.1CSPI02G23000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0030247 polysaccharide binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0008270 zinc ion binding