CSPI02G22070 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G22070
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	histidine-containing phosphotransfer protein 4-like
Location	Chr2: 19279318 .. 19281651 (-)
RNA-Seq Expression	CSPI02G22070
Synteny	CSPI02G22070

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACTCAAACCTTTTGCACCGTCAAGTTGCCTACACGAAGAGGTGCCTTTTTGAGCAGGTTTATTTTCAAATACAGGAAGTTCTTTCACTTTTCTTGTGTTTTCTCATGGTGCAAGTACTCTAGAACAGTATAACTTATACTTTGCATCAAGCATGTTGGAGAATCCTTGGTTCATAGTTTTCCGTACAGTGCATAAAGTTAACTACCAAGCTTTGTGTCTAATTCGAAGGGCAGGTTTAGCCTTTCGATGCATTAGCGGATGAGGAACCATTTTTCCTTCGTATAGAAGCCAAACTGAGCCATCTGTGTCCAAGAAAATATTCATGCCCTTCATCTTTGGACATAATAGAAGATTTGGTTACTGAACTTGTACTTGAGAGTAATGTAAAACTAAAACCACAAGACTCTGAATATTTGGTTAAAAAACAAACTTTTATTTATTTTCTCAAGTTGTTCCTCCAGGGTTTAAACGGTGAGATGGGCTAAACATGAAGTGAAAGGTCTCAGCTGGTTCTTGGTTGATTGGTTCATAATAGTACTGGATGATTACAGTTGTGCTATATGTTTTGGGCCAGCATAAATTATCAGCAGACCAGGAATAAGACAAAACTAAATTTATAAAATGCTAGGCCAGTTATTATGATTCTAGTGAATCTTAGCATTCACGTCCATATTGGATATTCCTTCAGTTTTGAAAATTGTGAATTTTTAGTTATATTTATTTATTTCAAATGATTGGCAAATAACTTCGACATAAATGGTTGAACCAGACAGAATTCTTTGTTGCAGGGTTATCTTGATCGTGATCAATTCACTCAGCTTGAAGAACTGCAGGATGATACTAACCCAAATTTTGCTGAAGAAATTGTGTCTTTATTCTACAATGATTCAGCCAGACTCATACGAAGCATTGAACAAACCATGTGAGTATAGAGCTTTAAAACTGATCAAAATCATTCTGGTGACCATTTATTCGAGCCTATTGTTTTACTGGCTTGTACAGGGCCAGTAAGCCTACCGATTTTGAAAAGCTTGACAACGACATGCACCAGTTCAAGGGTAGTAGCTCAAGGTAAAACCCATTAGGGAGAATTTCCTATACTATGGTAGTATGGAAATCTTTTATACCACCTCAACTTATTGTATGCCACGTCAACACACTTTTAAGAATAGGATAGAACCCAGTAACATTAATGTGGAGTTCGTTAGTAATTAAGAAGACAAAATTTATAGTGTGAACATAGGGAGTATGGTACTTCCTCATAATCTCGTAGAAGATTAATTGATCTAACTTTAGAGTTGAATATTTTTGTATAATGCATTAGAGATGATTATAAACATGTTTCCTTTCAAGTTAATAGCAATGGGAAAAGAGTAATCGTGTTTTGGCAGAAACTATTCTTTGGTACTTAATTCGTTGTTGATAAATCTATGTGAGGATGCATTTGATTGTTCTGGAATATTGGTTCAACGATGACGTCATTTTATCTTTAGTGAAAAGTTGAATCCTCCATGTGTATGAAGGTCTATTTCATGACAATGAAATATGGTGGTAGTGTCTCTGTCTGTTTAAGAAAATTGTTTTAATTCGCTTTAATTTTGTTGCTAGCATTGGAGCCAATAGGGTGACAGATGCATGTTCACAATTCAGAGAATACTGCTTGGCAGGAAATCTTGAAGGGTAATGATAATAATTTCTATATTATAAAGTTCATATATTAAGTCTATATTATAAAGTTCATATATTAAGTTTACACAAGCAAATGATAACTAAACTTGGTAACTTCTTTTCTTTTCTCCTTTTGTTATATAGATGCACAAGGAGTTTCCAGCAAGTGAAACAAGAGCACGAAATTCTGAAGAAGAAGCTTGATACCTATTTCCAGGTTTCTTTTTTCTCTCATTAATAATATAAACTGGTTAAATTAGGCAAAATAACCCAAATAACTTTGATTATTTTCATTTTTACCGTTGAAGTGTAAAGAATAAAAAGCAGGTGTGACATAGATGTATTCAATTTAATTCTATAATGATTATAATGATATCTCTTCTGTGATGAAATAAATGGAAAACAATGATTTTAATCTTCTATATGGCATCTCTTAATTTTAAATTTTTATACATAACTATGACATCAGTTTTTGTCATCTATTCTAGTTGAACATATATATATTTCAGAACAATTTCAAAGTTCAAAGACAAAGATTAAACATATAAAGATCTAGGAGCATATCGAAATCTACCGATACTGTACATATGTTATATGTATTTTTCTCATGCATATGCATTTGTTTCTATTTTCAGCTAGTTAGACAAGCTGGGAAAGGTTGA

mRNA sequence

ATGGACTCAAACCTTTTGCACCGTCAAGTTGCCTACACGAAGAGGTGCCTTTTTGAGCAGGGTTATCTTGATCGTGATCAATTCACTCAGCTTGAAGAACTGCAGGATGATACTAACCCAAATTTTGCTGAAGAAATTGTGTCTTTATTCTACAATGATTCAGCCAGACTCATACGAAGCATTGAACAAACCATGGCCAGTAAGCCTACCGATTTTGAAAAGCTTGACAACGACATGCACCAGTTCAAGGGTAGTAGCTCAAGCATTGGAGCCAATAGGGTGACAGATGCATGTTCACAATTCAGAGAATACTGCTTGGCAGGAAATCTTGAAGGATGCACAAGGAGTTTCCAGCAAGTGAAACAAGAGCACGAAATTCTGAAGAAGAAGCTTGATACCTATTTCCAGCTAGTTAGACAAGCTGGGAAAGGTTGA

Coding sequence (CDS)

ATGGACTCAAACCTTTTGCACCGTCAAGTTGCCTACACGAAGAGGTGCCTTTTTGAGCAGGGTTATCTTGATCGTGATCAATTCACTCAGCTTGAAGAACTGCAGGATGATACTAACCCAAATTTTGCTGAAGAAATTGTGTCTTTATTCTACAATGATTCAGCCAGACTCATACGAAGCATTGAACAAACCATGGCCAGTAAGCCTACCGATTTTGAAAAGCTTGACAACGACATGCACCAGTTCAAGGGTAGTAGCTCAAGCATTGGAGCCAATAGGGTGACAGATGCATGTTCACAATTCAGAGAATACTGCTTGGCAGGAAATCTTGAAGGATGCACAAGGAGTTTCCAGCAAGTGAAACAAGAGCACGAAATTCTGAAGAAGAAGCTTGATACCTATTTCCAGCTAGTTAGACAAGCTGGGAAAGGTTGA

Protein sequence

MDSNLLHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRSIEQTMASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQVKQEHEILKKKLDTYFQLVRQAGKG*

Homology

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BLAST of CSPI02G22070 vs. ExPASy Swiss-Prot
Match: Q6F303 (Pseudo histidine-containing phosphotransfer protein 5 OS=Oryza sativa subsp. japonica OX=39947 GN=PHP5 PE=2 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 7.0e-40
Identity = 79/142 (55.63%), Postives = 105/142 (73.94%), Query Frame = 0


Query: 1   MDSNLLHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60
           M+   L RQ A  K+ LF+QGYLD +QF Q+E+LQD+ NPNFAEE+VSLF+ DS R++ +
Sbjct: 1   MEYGNLRRQAASLKKSLFDQGYLD-EQFCQVEDLQDEANPNFAEEVVSLFFKDSTRVMLN 60

Query: 61  IEQTMASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQV 120
            EQ +   P DF + D  M Q KGS SSIGA+RV + C+ FR +C   N EGCTRSFQ+V
Sbjct: 61  FEQAIEKHPKDFARWDTHMQQLKGSCSSIGASRVKNECTSFRNFCGEENAEGCTRSFQKV 120

Query: 121 KQEHEILKKKLDTYFQLVRQAG 143
           K+EH +L++K ++YFQL+RQAG
Sbjct: 121 KREHAVLRQKWESYFQLLRQAG 141

[Close]

BLAST of CSPI02G22070 vs. ExPASy Swiss-Prot
Match: Q0DK78 (Pseudo histidine-containing phosphotransfer protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PHP2 PE=2 SV=3)

HSP 1 Score: 156.0 bits (393), Expect = 3.3e-37
Identity = 76/142 (53.52%), Postives = 106/142 (74.65%), Query Frame = 0


Query: 1   MDSNLLHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60
           M+ + L RQ+ + K+ LF+QGYLD +QF QLEELQD+++PNF EE+ +LF+ DS+RL+ +
Sbjct: 1   MEYSNLRRQIIFMKKNLFDQGYLD-EQFNQLEELQDESSPNFVEEVAALFFKDSSRLLTN 60

Query: 61  IEQTMASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQV 120
           IEQ +   P DF +LD+ + Q KGS SSIGA R+ + CS F+  C   NLEGC RS Q++
Sbjct: 61  IEQAIDKYPQDFYRLDSLVQQLKGSGSSIGALRMKNECSVFKVNCNDRNLEGCRRSLQKM 120

Query: 121 KQEHEILKKKLDTYFQLVRQAG 143
           K+EH  LK+KL++YFQL+RQ G
Sbjct: 121 KREHATLKQKLESYFQLLRQVG 141

[Close]

BLAST of CSPI02G22070 vs. ExPASy Swiss-Prot
Match: Q9LU15 (Histidine-containing phosphotransfer protein 4 OS=Arabidopsis thaliana OX=3702 GN=AHP4 PE=1 SV=2)

HSP 1 Score: 151.4 bits (381), Expect = 8.0e-36
Identity = 72/117 (61.54%), Postives = 92/117 (78.63%), Query Frame = 0


Query: 20  QGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRSIEQTMASKPTDFEKLDNDM 79
           QGYLD +QF +LEELQDD NPNF EE+ +L++ DSARLI +I+Q +     DF +LD+ M
Sbjct: 9   QGYLD-EQFMELEELQDDANPNFVEEVSALYFKDSARLINNIDQALERGSFDFNRLDSYM 68

Query: 80  HQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQVKQEHEILKKKLDTYFQ 137
           HQFKGSS+SIGA++V   C+ FREYC AGN EGC R+FQQ+K+EH  L+KKL+ YFQ
Sbjct: 69  HQFKGSSTSIGASKVKAECTTFREYCRAGNAEGCLRTFQQLKKEHSTLRKKLEHYFQ 124

[Close]

BLAST of CSPI02G22070 vs. ExPASy Swiss-Prot
Match: Q0JJE3 (Pseudo histidine-containing phosphotransfer protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PHP1 PE=3 SV=2)

HSP 1 Score: 147.9 bits (372), Expect = 8.9e-35
Identity = 69/123 (56.10%), Postives = 95/123 (77.24%), Query Frame = 0


Query: 20  QGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRSIEQTMASKPTDFEKLDNDM 79
           +GYLD +QF Q+E+LQD+ +PNF EE+V+LF+ DS RL+ +IEQ +   P DF + D  M
Sbjct: 56  KGYLD-EQFCQVEDLQDEASPNFVEEVVTLFFKDSGRLMSNIEQALEKYPRDFNRWDTYM 115

Query: 80  HQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQVKQEHEILKKKLDTYFQLVR 139
            Q KGS SSIGA+R+ + C  FR+ C  GN+EGC RSFQ+VK+EH +L++KL++YFQL+R
Sbjct: 116 QQLKGSCSSIGASRMKNECMSFRDSCGQGNVEGCMRSFQKVKREHAVLRQKLESYFQLLR 175

Query: 140 QAG 143
           QAG
Sbjct: 176 QAG 177

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BLAST of CSPI02G22070 vs. ExPASy Swiss-Prot
Match: Q9ZNV9 (Histidine-containing phosphotransfer protein 1 OS=Arabidopsis thaliana OX=3702 GN=AHP1 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 6.2e-28
Identity = 62/129 (48.06%), Postives = 91/129 (70.54%), Query Frame = 0


Query: 12  YTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRSIEQTMASKPTD 71
           YTK  LF +G LD  QF QL++LQD++NP+F  ++V+LF+ DS R++  +  ++  +  D
Sbjct: 13  YTK-SLFLEGILD-SQFLQLQQLQDESNPDFVSQVVTLFFQDSDRILNDLSLSLDQQVVD 72

Query: 72  FEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQVKQEHEILKKKL 131
           F+K+D  +HQ KGSSSSIGA RV +AC  FR +C   N+E C R  QQVKQE+ ++K +L
Sbjct: 73  FKKVDPHVHQLKGSSSSIGAQRVKNACVVFRSFCEQQNVEACHRCLQQVKQEYYLVKNRL 132

Query: 132 DTYFQLVRQ 141
           +T F+L +Q
Sbjct: 133 ETLFKLEQQ 139

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BLAST of CSPI02G22070 vs. ExPASy TrEMBL
Match: A0A0A0LLW8 (HPt domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G373410 PE=4 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 4.8e-76
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 0


Query: 1   MDSNLLHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60
           MDSNLLHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS
Sbjct: 1   MDSNLLHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60

Query: 61  IEQTMASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQV 120
           IEQTMASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQV
Sbjct: 61  IEQTMASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQV 120

Query: 121 KQEHEILKKKLDTYFQLVRQAGKG 145
           KQEHEILKKKLDTYFQLVRQAGKG
Sbjct: 121 KQEHEILKKKLDTYFQLVRQAGKG 144

[Close]

BLAST of CSPI02G22070 vs. ExPASy TrEMBL
Match: A0A5A7VA52 (Histidine-containing phosphotransfer protein 4-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003820 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.7e-73
Identity = 140/144 (97.22%), Postives = 141/144 (97.92%), Query Frame = 0


Query: 1   MDSNLLHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60
           MDSNLLHRQVAYTKRC FEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS
Sbjct: 1   MDSNLLHRQVAYTKRCFFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60

Query: 61  IEQTMASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQV 120
           IEQT+ASKPTDFEKLDN MHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGC RSFQQV
Sbjct: 61  IEQTLASKPTDFEKLDNYMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCMRSFQQV 120

Query: 121 KQEHEILKKKLDTYFQLVRQAGKG 145
           KQEHEILKKKLDTYFQLVRQAGKG
Sbjct: 121 KQEHEILKKKLDTYFQLVRQAGKG 144

[Close]

BLAST of CSPI02G22070 vs. ExPASy TrEMBL
Match: A0A6J1KJT4 (histidine-containing phosphotransfer protein 4-like OS=Cucurbita maxima OX=3661 GN=LOC111496354 PE=4 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 3.3e-69
Identity = 133/142 (93.66%), Postives = 136/142 (95.77%), Query Frame = 0


Query: 1   MDSNLLHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60
           MDS+LL RQVAYTKRC FEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS
Sbjct: 1   MDSSLLQRQVAYTKRCFFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60

Query: 61  IEQTMASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQV 120
           IEQTM SKPTDFEKLDN MHQFKGSSSSIGANRVT+ACSQFREYCLAGNLEGC RSFQ+V
Sbjct: 61  IEQTMVSKPTDFEKLDNYMHQFKGSSSSIGANRVTEACSQFREYCLAGNLEGCIRSFQEV 120

Query: 121 KQEHEILKKKLDTYFQLVRQAG 143
           KQEHEILKKKLDTYFQL RQAG
Sbjct: 121 KQEHEILKKKLDTYFQLARQAG 142

[Close]

BLAST of CSPI02G22070 vs. ExPASy TrEMBL
Match: A0A1S3BD23 (histidine-containing phosphotransfer protein 4-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488557 PE=4 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 7.5e-69
Identity = 132/137 (96.35%), Postives = 134/137 (97.81%), Query Frame = 0


Query: 1   MDSNLLHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60
           MDSNLLHRQVAYTKRC FEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS
Sbjct: 1   MDSNLLHRQVAYTKRCFFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60

Query: 61  IEQTMASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQV 120
           IEQT+ASKPTDFEKLDN MHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGC RSFQQV
Sbjct: 61  IEQTLASKPTDFEKLDNYMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCMRSFQQV 120

Query: 121 KQEHEILKKKLDTYFQL 138
           KQEHEILKKKLDTYFQ+
Sbjct: 121 KQEHEILKKKLDTYFQV 137

[Close]

BLAST of CSPI02G22070 vs. ExPASy TrEMBL
Match: A0A6J1GGG6 (histidine-containing phosphotransfer protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC111453979 PE=4 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 9.7e-69
Identity = 132/142 (92.96%), Postives = 136/142 (95.77%), Query Frame = 0


Query: 1   MDSNLLHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60
           MDS+LL RQVAYTKRC FEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS
Sbjct: 1   MDSSLLQRQVAYTKRCFFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60

Query: 61  IEQTMASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQV 120
           IEQT+ SKPTDFEKLDN MHQFKGSSSSIGANRVT+ACSQFREYCLAGNLEGC RSFQ+V
Sbjct: 61  IEQTIVSKPTDFEKLDNYMHQFKGSSSSIGANRVTEACSQFREYCLAGNLEGCIRSFQEV 120

Query: 121 KQEHEILKKKLDTYFQLVRQAG 143
           KQEHEILKKKLDTYFQL RQAG
Sbjct: 121 KQEHEILKKKLDTYFQLARQAG 142

[Close]

BLAST of CSPI02G22070 vs. NCBI nr
Match: XP_004148397.1 (histidine-containing phosphotransfer protein 4 isoform X1 [Cucumis sativus] >XP_031737228.1 histidine-containing phosphotransfer protein 4 isoform X1 [Cucumis sativus] >KGN62798.1 hypothetical protein Csa_022235 [Cucumis sativus])

HSP 1 Score: 293.5 bits (750), Expect = 1.0e-75
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 0


Query: 1   MDSNLLHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60
           MDSNLLHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS
Sbjct: 1   MDSNLLHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60

Query: 61  IEQTMASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQV 120
           IEQTMASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQV
Sbjct: 61  IEQTMASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQV 120

Query: 121 KQEHEILKKKLDTYFQLVRQAGKG 145
           KQEHEILKKKLDTYFQLVRQAGKG
Sbjct: 121 KQEHEILKKKLDTYFQLVRQAGKG 144

[Close]

BLAST of CSPI02G22070 vs. NCBI nr
Match: KAA0065053.1 (histidine-containing phosphotransfer protein 4-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 285.0 bits (728), Expect = 3.5e-73
Identity = 140/144 (97.22%), Postives = 141/144 (97.92%), Query Frame = 0


Query: 1   MDSNLLHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60
           MDSNLLHRQVAYTKRC FEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS
Sbjct: 1   MDSNLLHRQVAYTKRCFFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60

Query: 61  IEQTMASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQV 120
           IEQT+ASKPTDFEKLDN MHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGC RSFQQV
Sbjct: 61  IEQTLASKPTDFEKLDNYMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCMRSFQQV 120

Query: 121 KQEHEILKKKLDTYFQLVRQAGKG 145
           KQEHEILKKKLDTYFQLVRQAGKG
Sbjct: 121 KQEHEILKKKLDTYFQLVRQAGKG 144

[Close]

BLAST of CSPI02G22070 vs. NCBI nr
Match: XP_011649724.1 (histidine-containing phosphotransfer protein 4 isoform X2 [Cucumis sativus] >XP_031737229.1 histidine-containing phosphotransfer protein 4 isoform X2 [Cucumis sativus])

HSP 1 Score: 278.1 bits (710), Expect = 4.3e-71
Identity = 136/137 (99.27%), Postives = 137/137 (100.00%), Query Frame = 0


Query: 1   MDSNLLHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60
           MDSNLLHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS
Sbjct: 1   MDSNLLHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60

Query: 61  IEQTMASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQV 120
           IEQTMASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQV
Sbjct: 61  IEQTMASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQV 120

Query: 121 KQEHEILKKKLDTYFQL 138
           KQEHEILKKKLDTYFQ+
Sbjct: 121 KQEHEILKKKLDTYFQV 137

[Close]

BLAST of CSPI02G22070 vs. NCBI nr
Match: XP_023537128.1 (histidine-containing phosphotransfer protein 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 272.7 bits (696), Expect = 1.8e-69
Identity = 134/143 (93.71%), Postives = 137/143 (95.80%), Query Frame = 0


Query: 1   MDSNLLHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60
           MDS+LL RQVAYTKRC FEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS
Sbjct: 1   MDSSLLQRQVAYTKRCFFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60

Query: 61  IEQTMASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQV 120
           IEQTM SKPTDFEKLDN MHQFKGSSSSIGANRVT+ACSQFREYCLAGNLEGC RSFQ+V
Sbjct: 61  IEQTMVSKPTDFEKLDNYMHQFKGSSSSIGANRVTEACSQFREYCLAGNLEGCIRSFQEV 120

Query: 121 KQEHEILKKKLDTYFQLVRQAGK 144
           KQEHEILKKKLDTYFQL RQAGK
Sbjct: 121 KQEHEILKKKLDTYFQLARQAGK 143

[Close]

BLAST of CSPI02G22070 vs. NCBI nr
Match: XP_023002542.1 (histidine-containing phosphotransfer protein 4-like [Cucurbita maxima])

HSP 1 Score: 270.8 bits (691), Expect = 6.9e-69
Identity = 133/142 (93.66%), Postives = 136/142 (95.77%), Query Frame = 0


Query: 1   MDSNLLHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60
           MDS+LL RQVAYTKRC FEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS
Sbjct: 1   MDSSLLQRQVAYTKRCFFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRS 60

Query: 61  IEQTMASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQV 120
           IEQTM SKPTDFEKLDN MHQFKGSSSSIGANRVT+ACSQFREYCLAGNLEGC RSFQ+V
Sbjct: 61  IEQTMVSKPTDFEKLDNYMHQFKGSSSSIGANRVTEACSQFREYCLAGNLEGCIRSFQEV 120

Query: 121 KQEHEILKKKLDTYFQLVRQAG 143
           KQEHEILKKKLDTYFQL RQAG
Sbjct: 121 KQEHEILKKKLDTYFQLARQAG 142

[Close]

BLAST of CSPI02G22070 vs. TAIR 10
Match: AT3G16360.2 (HPT phosphotransmitter 4 )

HSP 1 Score: 174.9 bits (442), Expect = 4.8e-44
Identity = 84/137 (61.31%), Postives = 107/137 (78.10%), Query Frame = 0


Query: 6   LHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRSIEQTM 65
           + RQVA  K+ LF+QGYLD +QF +LEELQDD NPNF EE+ +L++ DSARLI +I+Q +
Sbjct: 1   MQRQVALIKQSLFDQGYLD-EQFMELEELQDDANPNFVEEVSALYFKDSARLINNIDQAL 60

Query: 66  ASKPTDFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQVKQEHE 125
                DF +LD+ MHQFKGSS+SIGA++V   C+ FREYC AGN EGC R+FQQ+K+EH 
Sbjct: 61  ERGSFDFNRLDSYMHQFKGSSTSIGASKVKAECTTFREYCRAGNAEGCLRTFQQLKKEHS 120

Query: 126 ILKKKLDTYFQLVRQAG 143
            L+KKL+ YFQL RQAG
Sbjct: 121 TLRKKLEHYFQLARQAG 136

[Close]

BLAST of CSPI02G22070 vs. TAIR 10
Match: AT3G16360.1 (HPT phosphotransmitter 4 )

HSP 1 Score: 151.4 bits (381), Expect = 5.7e-37
Identity = 72/117 (61.54%), Postives = 92/117 (78.63%), Query Frame = 0


Query: 20  QGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRSIEQTMASKPTDFEKLDNDM 79
           QGYLD +QF +LEELQDD NPNF EE+ +L++ DSARLI +I+Q +     DF +LD+ M
Sbjct: 9   QGYLD-EQFMELEELQDDANPNFVEEVSALYFKDSARLINNIDQALERGSFDFNRLDSYM 68

Query: 80  HQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQVKQEHEILKKKLDTYFQ 137
           HQFKGSS+SIGA++V   C+ FREYC AGN EGC R+FQQ+K+EH  L+KKL+ YFQ
Sbjct: 69  HQFKGSSTSIGASKVKAECTTFREYCRAGNAEGCLRTFQQLKKEHSTLRKKLEHYFQ 124

[Close]

BLAST of CSPI02G22070 vs. TAIR 10
Match: AT3G21510.1 (histidine-containing phosphotransmitter 1 )

HSP 1 Score: 125.2 bits (313), Expect = 4.4e-29
Identity = 62/129 (48.06%), Postives = 91/129 (70.54%), Query Frame = 0


Query: 12  YTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRSIEQTMASKPTD 71
           YTK  LF +G LD  QF QL++LQD++NP+F  ++V+LF+ DS R++  +  ++  +  D
Sbjct: 13  YTK-SLFLEGILD-SQFLQLQQLQDESNPDFVSQVVTLFFQDSDRILNDLSLSLDQQVVD 72

Query: 72  FEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQVKQEHEILKKKL 131
           F+K+D  +HQ KGSSSSIGA RV +AC  FR +C   N+E C R  QQVKQE+ ++K +L
Sbjct: 73  FKKVDPHVHQLKGSSSSIGAQRVKNACVVFRSFCEQQNVEACHRCLQQVKQEYYLVKNRL 132

Query: 132 DTYFQLVRQ 141
           +T F+L +Q
Sbjct: 133 ETLFKLEQQ 139

[Close]

BLAST of CSPI02G22070 vs. TAIR 10
Match: AT3G29350.1 (histidine-containing phosphotransmitter 2 )

HSP 1 Score: 116.3 bits (290), Expect = 2.0e-26
Identity = 62/136 (45.59%), Postives = 87/136 (63.97%), Query Frame = 0


Query: 6   LHRQVAYTKRCLFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRSIEQTM 65
           L RQ       L++QG+LD DQFT+L++LQDD +P+F  E++SLF+ D  +LI ++ + +
Sbjct: 8   LQRQFRDYTISLYQQGFLD-DQFTELKKLQDDGSPDFVSEVLSLFFEDCVKLISNMARAL 67

Query: 66  ASKPT-DFEKLDNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQVKQEH 125
            +  T DF ++   +HQ KGSSSS+GA RV   C  F+E C A N EGC R  QQV  E+
Sbjct: 68  DTTGTVDFSQVGASVHQLKGSSSSVGAKRVKTLCVSFKECCEAKNYEGCVRCLQQVDIEY 127

Query: 126 EILKKKLDTYFQLVRQ 141
           + LK KL   F L +Q
Sbjct: 128 KALKTKLQDMFNLEKQ 142

[Close]

BLAST of CSPI02G22070 vs. TAIR 10
Match: AT1G03430.1 (histidine-containing phosphotransfer factor 5 )

HSP 1 Score: 112.5 bits (280), Expect = 2.9e-25
Identity = 56/125 (44.80%), Postives = 86/125 (68.80%), Query Frame = 0


Query: 17  LFEQGYLDRDQFTQLEELQDDTNPNFAEEIVSLFYNDSARLIRSIEQTMASKP-TDFEKL 76
           L++QG+LD +QF++L +LQD+  P+F  E+VSLF++D ++LI ++  ++      DF+++
Sbjct: 20  LYQQGFLD-NQFSELRKLQDEGTPDFVAEVVSLFFDDCSKLINTMSISLERPDNVDFKQV 79

Query: 77  DNDMHQFKGSSSSIGANRVTDACSQFREYCLAGNLEGCTRSFQQVKQEHEILKKKLDTYF 136
           D+ +HQ KGSSSS+GA RV + C  F+E C   N EGC R  QQV  E+++LK KL   F
Sbjct: 80  DSGVHQLKGSSSSVGARRVKNVCISFKECCDVQNREGCLRCLQQVDYEYKMLKTKLQDLF 139

Query: 137 QLVRQ 141
            L +Q
Sbjct: 140 NLEKQ 143

The following BLAST results are available for this feature:

BLAST of CSPI02G22070 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6F303	7.0e-40	55.63	Pseudo histidine-containing phosphotransfer protein 5 OS=Oryza sativa subsp. jap...	[more]
Q0DK78	3.3e-37	53.52	Pseudo histidine-containing phosphotransfer protein 2 OS=Oryza sativa subsp. jap...	[more]
Q9LU15	8.0e-36	61.54	Histidine-containing phosphotransfer protein 4 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q0JJE3	8.9e-35	56.10	Pseudo histidine-containing phosphotransfer protein 1 OS=Oryza sativa subsp. jap...	[more]
Q9ZNV9	6.2e-28	48.06	Histidine-containing phosphotransfer protein 1 OS=Arabidopsis thaliana OX=3702 G...	[more]

BLAST of CSPI02G22070 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LLW8	4.8e-76	100.00	HPt domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G373410 PE=4 SV...	[more]
A0A5A7VA52	1.7e-73	97.22	Histidine-containing phosphotransfer protein 4-like isoform X1 OS=Cucumis melo v...	[more]
A0A6J1KJT4	3.3e-69	93.66	histidine-containing phosphotransfer protein 4-like OS=Cucurbita maxima OX=3661 ...	[more]
A0A1S3BD23	7.5e-69	96.35	histidine-containing phosphotransfer protein 4-like isoform X1 OS=Cucumis melo O...	[more]
A0A6J1GGG6	9.7e-69	92.96	histidine-containing phosphotransfer protein 4-like OS=Cucurbita moschata OX=366...	[more]

BLAST of CSPI02G22070 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148397.1	1.0e-75	100.00	histidine-containing phosphotransfer protein 4 isoform X1 [Cucumis sativus] >XP_...	[more]
KAA0065053.1	3.5e-73	97.22	histidine-containing phosphotransfer protein 4-like isoform X1 [Cucumis melo var...	[more]
XP_011649724.1	4.3e-71	99.27	histidine-containing phosphotransfer protein 4 isoform X2 [Cucumis sativus] >XP_...	[more]
XP_023537128.1	1.8e-69	93.71	histidine-containing phosphotransfer protein 4-like [Cucurbita pepo subsp. pepo]	[more]
XP_023002542.1	6.9e-69	93.66	histidine-containing phosphotransfer protein 4-like [Cucurbita maxima]	[more]

BLAST of CSPI02G22070 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G16360.2	4.8e-44	61.31	HPT phosphotransmitter 4	[more]
AT3G16360.1	5.7e-37	61.54	HPT phosphotransmitter 4	[more]
AT3G21510.1	4.4e-29	48.06	histidine-containing phosphotransmitter 1	[more]
AT3G29350.1	2.0e-26	45.59	histidine-containing phosphotransmitter 2	[more]
AT1G03430.1	2.9e-25	44.80	histidine-containing phosphotransfer factor 5	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008207	Signal transduction histidine kinase, phosphotransfer (Hpt) domain	PFAM	PF01627	Hpt	coord: 45..127 e-value: 3.2E-10 score: 40.1
IPR008207	Signal transduction histidine kinase, phosphotransfer (Hpt) domain	PROSITE	PS50894	HPT	coord: 39..134 score: 13.587135
IPR008207	Signal transduction histidine kinase, phosphotransfer (Hpt) domain	CDD	cd00088	HPT	coord: 44..123 e-value: 1.67784E-7 score: 44.6798
IPR036641	HPT domain superfamily	GENE3D	1.20.120.160	HPT domain	coord: 2..143 e-value: 3.8E-48 score: 165.1
IPR036641	HPT domain superfamily	SUPERFAMILY	47226	Histidine-containing phosphotransfer domain, HPT domain	coord: 19..140
None	No IPR available	PANTHER	PTHR28242:SF51	PSEUDO HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN 2-LIKE	coord: 3..142
None	No IPR available	PANTHER	PTHR28242	FAMILY NOT NAMED	coord: 3..142

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G22070.1	CSPI02G22070.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009736	cytokinin-activated signaling pathway
biological_process	GO:0000160	phosphorelay signal transduction system
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005634	nucleus
molecular_function	GO:0009927	histidine phosphotransfer kinase activity
molecular_function	GO:0043424	protein histidine kinase binding