CSPI02G21740 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G21740
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionMitochondrial uncoupling protein 5
LocationChr2: 19035248 .. 19036585 (+)
RNA-Seq ExpressionCSPI02G21740
SyntenyCSPI02G21740
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGAAGGGCTTAATATAAATAAAGTCAGAGTCAAAGGAAGTCAAGAAGTGGGCAGTCGCATTAGGGCATCCCCTTCTTCCTTGGACCCACAGCCGTCTCCGGTCGCCATTTATTCTTCAACGCGTAAATGTAAGCTTCTCCGATCCCACATTCTTTCTCCTTCCTATATAATGAATTTAATCTTCTTCTCTTCACCATTACCAATTACTTCCCTTCTCAATTCTTCTAATCACTTTTCTTCTTTCTCAAATCCTCAATTTCAGTCTCAGAATGGGTTTCAAGGGATTTGCCGAAGGAGGAATCGCTTCCATTGTTGCCGGTTGCTCTACTCACCCACTTGATCTAATCAAAGTTCGGATGCAATTAGATGGCGAGAAACCGCCACTTCCAAATCTCCGCCCTGCCCTAGCTTTCAATGCTTCTCGTTCCCTTGTTGCGCCAGAATCTTTTCACATTCCGCCGCCCCAACCTCCGCGTGTCGGACCCATATCGGTCGGCGTGCGGATTGTCCAGTCCGAAGGCGTTGCCGCTCTGTTTTCCGGTGTCTCTGCAACTGTTCTCCGACAAACTCTTTACTCCACCACTCGGATGGGTCTTTACGATATCCTCAAGACTAAATGGTCCGATCCAGATTCCGGAAGTATGCCTCTGACTCGGAAAATCACCGCGGGGTTAATCGCCGGTGGGATTGGCGCAGCGGTCGGGAACCCGGCCGATGTCGCAATGGTGAGAATGCAGGCCGATGGACGACTTCCAGTGGCTCAGCGGCGGAATTACGCCGGAGTGGTGGACGCGATCACGAGAATGTCGAAGCAAGAGGGTATTACTAGTCTATGGCGCGGGTCGGCGCTTACGGTGAACCGAGCGATGATCGTGACGGCGGCGCAGTTGGCGTCGTACGATCAGATAAAAGAGACGATATTGGAGAAGGGTGTGATGAAGGACGGACTGGGGACACACGTGACAGCAAGTTTCGCGGCGGGGTTTGTGGCGGCGGTGGCATCGAATCCGGTGGATGTGATAAAGACGAGAGTCATGAATATGAAGGTGGAGGCAGGGGAGACGGCACCGTACAGTGGGGCGTTGGACTGTGCAATGAAGACGGTGAAGGCGGAAGGGCCGATGGCTCTTTACAAAGGATTTATTCCGACGATTTCACGGCAAGGGCCTTTCACGGTGGTCTTGTTTGTCACACTTGAACAAGTCAGAAAAATTTTCAACCAATTTTGATTTGACGATGGGTACTTTATATTATAAACTGTTCCCAAATTCTTTATATTCAATTTTAATTAATACATTTCAGATACACTTTTACATCTATCCTGCTTCTTCTTTAC

mRNA sequence

GAAGAAGGGCTTAATATAAATAAAGTCAGAGTCAAAGGAAGTCAAGAAGTGGGCAGTCGCATTAGGGCATCCCCTTCTTCCTTGGACCCACAGCCGTCTCCGGTCGCCATTTATTCTTCAACGCGTAAATTCTCAGAATGGGTTTCAAGGGATTTGCCGAAGGAGGAATCGCTTCCATTGTTGCCGGTTGCTCTACTCACCCACTTGATCTAATCAAAGTTCGGATGCAATTAGATGGCGAGAAACCGCCACTTCCAAATCTCCGCCCTGCCCTAGCTTTCAATGCTTCTCGTTCCCTTGTTGCGCCAGAATCTTTTCACATTCCGCCGCCCCAACCTCCGCGTGTCGGACCCATATCGGTCGGCGTGCGGATTGTCCAGTCCGAAGGCGTTGCCGCTCTGTTTTCCGGTGTCTCTGCAACTGTTCTCCGACAAACTCTTTACTCCACCACTCGGATGGGTCTTTACGATATCCTCAAGACTAAATGGTCCGATCCAGATTCCGGAAGTATGCCTCTGACTCGGAAAATCACCGCGGGGTTAATCGCCGGTGGGATTGGCGCAGCGGTCGGGAACCCGGCCGATGTCGCAATGGTGAGAATGCAGGCCGATGGACGACTTCCAGTGGCTCAGCGGCGGAATTACGCCGGAGTGGTGGACGCGATCACGAGAATGTCGAAGCAAGAGGGTATTACTAGTCTATGGCGCGGGTCGGCGCTTACGGTGAACCGAGCGATGATCGTGACGGCGGCGCAGTTGGCGTCGTACGATCAGATAAAAGAGACGATATTGGAGAAGGGTGTGATGAAGGACGGACTGGGGACACACGTGACAGCAAGTTTCGCGGCGGGGTTTGTGGCGGCGGTGGCATCGAATCCGGTGGATGTGATAAAGACGAGAGTCATGAATATGAAGGTGGAGGCAGGGGAGACGGCACCGTACAGTGGGGCGTTGGACTGTGCAATGAAGACGGTGAAGGCGGAAGGGCCGATGGCTCTTTACAAAGGATTTATTCCGACGATTTCACGGCAAGGGCCTTTCACGGTGGTCTTGTTTGTCACACTTGAACAAGTCAGAAAAATTTTCAACCAATTTTGATTTGACGATGGGTACTTTATATTATAAACTGTTCCCAAATTCTTTATATTCAATTTTAATTAATACATTTCAGATACACTTTTACATCTATCCTGCTTCTTCTTTAC

Coding sequence (CDS)

ATGGGTTTCAAGGGATTTGCCGAAGGAGGAATCGCTTCCATTGTTGCCGGTTGCTCTACTCACCCACTTGATCTAATCAAAGTTCGGATGCAATTAGATGGCGAGAAACCGCCACTTCCAAATCTCCGCCCTGCCCTAGCTTTCAATGCTTCTCGTTCCCTTGTTGCGCCAGAATCTTTTCACATTCCGCCGCCCCAACCTCCGCGTGTCGGACCCATATCGGTCGGCGTGCGGATTGTCCAGTCCGAAGGCGTTGCCGCTCTGTTTTCCGGTGTCTCTGCAACTGTTCTCCGACAAACTCTTTACTCCACCACTCGGATGGGTCTTTACGATATCCTCAAGACTAAATGGTCCGATCCAGATTCCGGAAGTATGCCTCTGACTCGGAAAATCACCGCGGGGTTAATCGCCGGTGGGATTGGCGCAGCGGTCGGGAACCCGGCCGATGTCGCAATGGTGAGAATGCAGGCCGATGGACGACTTCCAGTGGCTCAGCGGCGGAATTACGCCGGAGTGGTGGACGCGATCACGAGAATGTCGAAGCAAGAGGGTATTACTAGTCTATGGCGCGGGTCGGCGCTTACGGTGAACCGAGCGATGATCGTGACGGCGGCGCAGTTGGCGTCGTACGATCAGATAAAAGAGACGATATTGGAGAAGGGTGTGATGAAGGACGGACTGGGGACACACGTGACAGCAAGTTTCGCGGCGGGGTTTGTGGCGGCGGTGGCATCGAATCCGGTGGATGTGATAAAGACGAGAGTCATGAATATGAAGGTGGAGGCAGGGGAGACGGCACCGTACAGTGGGGCGTTGGACTGTGCAATGAAGACGGTGAAGGCGGAAGGGCCGATGGCTCTTTACAAAGGATTTATTCCGACGATTTCACGGCAAGGGCCTTTCACGGTGGTCTTGTTTGTCACACTTGAACAAGTCAGAAAAATTTTCAACCAATTTTGA

Protein sequence

MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESFHIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDPDSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMSKQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFVAAVASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGPFTVVLFVTLEQVRKIFNQF*
Homology
BLAST of CSPI02G21740 vs. ExPASy Swiss-Prot
Match: Q9SJY5 (Mitochondrial uncoupling protein 5 OS=Arabidopsis thaliana OX=3702 GN=PUMP5 PE=2 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 3.6e-129
Identity = 237/320 (74.06%), Postives = 273/320 (85.31%), Query Frame = 0

Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPL-PNLRPALAFNASRSLVAPES 60
           MG KGFAEGGIASIVAGCSTHPLDLIKVRMQL GE  P+  NLRPALAF  S ++ AP  
Sbjct: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLQGESAPIQTNLRPALAFQTSTTVNAP-- 60

Query: 61  FHIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSD 120
                  P RVG I VG R+++ EG+ ALFSGVSATVLRQTLYSTTRMGLYDI+K +W+D
Sbjct: 61  -------PLRVGVIGVGSRLIREEGMRALFSGVSATVLRQTLYSTTRMGLYDIIKGEWTD 120

Query: 121 PDSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRM 180
           P++ +MPL +KI AG IAG IGAAVGNPADVAMVRMQADGRLP+  RRNY  V+DAIT+M
Sbjct: 121 PETKTMPLMKKIGAGAIAGAIGAAVGNPADVAMVRMQADGRLPLTDRRNYKSVLDAITQM 180

Query: 181 SKQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGF 240
            + EG+TSLWRGS+LT+NRAM+VT++QLASYD +KETILEKG++KDGLGTHV+ASFAAGF
Sbjct: 181 IRGEGVTSLWRGSSLTINRAMLVTSSQLASYDSVKETILEKGLLKDGLGTHVSASFAAGF 240

Query: 241 VAAVASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQG 300
           VA+VASNPVDVIKTRVMNMKV AG   PY GA+DCA+KTVKAEG M+LYKGFIPT+SRQ 
Sbjct: 241 VASVASNPVDVIKTRVMNMKVVAGVAPPYKGAVDCALKTVKAEGIMSLYKGFIPTVSRQA 300

Query: 301 PFTVVLFVTLEQVRKIFNQF 320
           PFTVVLFVTLEQV+K+F  +
Sbjct: 301 PFTVVLFVTLEQVKKLFKDY 311

BLAST of CSPI02G21740 vs. ExPASy Swiss-Prot
Match: Q9SB52 (Mitochondrial uncoupling protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUMP4 PE=2 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 2.0e-127
Identity = 242/322 (75.16%), Postives = 267/322 (82.92%), Query Frame = 0

Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPN---LRPALAFNASRSLVAP 60
           MG K F EGGIAS++AGCSTHPLDLIKVR+QL GE P       LRPALAF  S    +P
Sbjct: 1   MGVKSFVEGGIASVIAGCSTHPLDLIKVRLQLHGEAPSTTTVTLLRPALAFPNS----SP 60

Query: 61  ESFHIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKW 120
            +F       P+VGPIS+G+ IV+SEG AALFSGVSAT+LRQTLYSTTRMGLY++LK KW
Sbjct: 61  AAFLETTSSVPKVGPISLGINIVKSEGAAALFSGVSATLLRQTLYSTTRMGLYEVLKNKW 120

Query: 121 SDPDSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAIT 180
           +DP+SG + L+RKI AGL+AGGIGAAVGNPADVAMVRMQADGRLP+AQRRNYAGV DAI 
Sbjct: 121 TDPESGKLNLSRKIGAGLVAGGIGAAVGNPADVAMVRMQADGRLPLAQRRNYAGVGDAIR 180

Query: 181 RMSKQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAA 240
            M K EG+TSLWRGSALT+NRAMIVTAAQLASYDQ KE ILE GVM DGLGTHV ASFAA
Sbjct: 181 SMVKGEGVTSLWRGSALTINRAMIVTAAQLASYDQFKEGILENGVMNDGLGTHVVASFAA 240

Query: 241 GFVAAVASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTVKAEGPMALYKGFIPTISR 300
           GFVA+VASNPVDVIKTRVMNMKV A     Y GA DCA+KTVKAEG MALYKGF+PT+ R
Sbjct: 241 GFVASVASNPVDVIKTRVMNMKVGA-----YDGAWDCAVKTVKAEGAMALYKGFVPTVCR 300

Query: 301 QGPFTVVLFVTLEQVRKIFNQF 320
           QGPFTVVLFVTLEQVRK+   F
Sbjct: 301 QGPFTVVLFVTLEQVRKLLRDF 313

BLAST of CSPI02G21740 vs. ExPASy Swiss-Prot
Match: Q9FY68 (Mitochondrial uncoupling protein 6 OS=Arabidopsis thaliana OX=3702 GN=PUMP6 PE=2 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 3.5e-100
Identity = 200/334 (59.88%), Postives = 241/334 (72.16%), Query Frame = 0

Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGE------KPPLPNL-----------R 60
           MGFK F EGGIA+I+AG  THPLDLIKVRMQL GE      + P PNL           R
Sbjct: 1   MGFKPFLEGGIAAIIAGALTHPLDLIKVRMQLQGEHSFSLDQNPNPNLSLDHNLPVKPYR 60

Query: 61  PALAFNASRSLVAPESFHIPPPQPPR---VGPISVGVRIVQSEGVAALFSGVSATVLRQT 120
           P  A ++    ++    HI  P       + P +VG  IV++EG AALFSGVSAT+LRQ 
Sbjct: 61  PVFALDSLIGSISLLPLHIHAPSSSTRSVMTPFAVGAHIVKTEGPAALFSGVSATILRQM 120

Query: 121 LYSTTRMGLYDILKTKWSDPDSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGR 180
           LYS TRMG+YD LK +W+D  +G+ PL  KITAGLIAG +G+ VGNPADVAMVRMQADG 
Sbjct: 121 LYSATRMGIYDFLKRRWTDQLTGNFPLVTKITAGLIAGAVGSVVGNPADVAMVRMQADGS 180

Query: 181 LPVAQRRNYAGVVDAITRMSKQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEK 240
           LP+ +RRNY  VVDAI R+++QEG++SLWRGS LTVNRAMIVTA+QLA+YD +KE ++  
Sbjct: 181 LPLNRRRNYKSVVDAIDRIARQEGVSSLWRGSWLTVNRAMIVTASQLATYDHVKEILVAG 240

Query: 241 G-VMKDGLGTHVTASFAAGFVAAVASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTV 300
           G     G+GTHV ASFAAG VAAVASNP+DV+KTR+MN   E      Y G LDCA+K V
Sbjct: 241 GRGTPGGIGTHVAASFAAGIVAAVASNPIDVVKTRMMNADKEI-----YGGPLDCAVKMV 300

Query: 301 KAEGPMALYKGFIPTISRQGPFTVVLFVTLEQVR 314
             EGPMALYKG +PT +RQGPFT++LF+TLEQVR
Sbjct: 301 AEEGPMALYKGLVPTATRQGPFTMILFLTLEQVR 329

BLAST of CSPI02G21740 vs. ExPASy Swiss-Prot
Match: Q54PY7 (Probable mitochondrial 2-oxoglutarate/malate carrier protein OS=Dictyostelium discoideum OX=44689 GN=ucpC PE=3 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.4e-64
Identity = 137/315 (43.49%), Postives = 189/315 (60.00%), Query Frame = 0

Query: 4   KGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESFHIP 63
           K F  GG+A +++   THP+D +KVRMQL GE   +                        
Sbjct: 26  KQFVIGGLAGMLSSAFTHPIDSLKVRMQLQGEGTGV------------------------ 85

Query: 64  PPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDPDSG 123
               P+ G + + V I Q+EG   L+ G+SA++LRQ  Y+TTR GLYD++K   +  D  
Sbjct: 86  ---GPKRGALKMLVHINQTEGFFTLYKGLSASLLRQATYTTTRFGLYDLIKDIVAKDDK- 145

Query: 124 SMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMSKQE 183
            +P T+KI  G+++G  GA VG PAD+ MVRMQADG+LP   RRNY  V D I R+SK+E
Sbjct: 146 PLPFTQKIMVGMLSGAGGAIVGTPADLTMVRMQADGKLPFNLRRNYKNVFDGIFRISKEE 205

Query: 184 GITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFVAAV 243
           GI SLW+G +  + RAM +TA Q++SYDQ K+ +L  G   D + TH+ AS  A FVAAV
Sbjct: 206 GIISLWKGCSPNLIRAMFMTAGQVSSYDQTKQLMLASGYFHDDIKTHLIASTTAAFVAAV 265

Query: 244 ASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGPFTV 303
           A++P+DVIKTR+MN          Y G  DC  KT++AEG  A YKGF P   R GP T+
Sbjct: 266 ATSPLDVIKTRIMNSPKTVTGELQYKGTFDCLSKTLRAEGFKAFYKGFNPYFMRLGPQTI 312

Query: 304 VLFVTLEQVRKIFNQ 319
           + F+ +EQ+  ++ +
Sbjct: 326 LTFIFVEQLNILWKK 312

BLAST of CSPI02G21740 vs. ExPASy Swiss-Prot
Match: P90992 (Mitochondrial 2-oxoglutarate/malate carrier protein OS=Caenorhabditis elegans OX=6239 GN=misc-1 PE=1 SV=2)

HSP 1 Score: 229.2 bits (583), Expect = 6.7e-59
Identity = 132/314 (42.04%), Postives = 183/314 (58.28%), Query Frame = 0

Query: 6   FAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESFHIPPP 65
           FA GG A + A     PLDL+K RMQL G                +       S H    
Sbjct: 13  FAFGGTAGMGATLVVQPLDLVKNRMQLSG---------------TTGKKEYRSSMH---- 72

Query: 66  QPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDPDSGSM 125
                        I+++EGV A+++G+SA +LRQ  Y+TTR+G Y  L  ++++ D   +
Sbjct: 73  ---------ALTSIMKNEGVFAVYNGLSAGLLRQATYTTTRLGTYAFLLERFTEKDK-PL 132

Query: 126 PLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMSKQEGI 185
               K   G+ AGGIG+ VG PA++A++RM  DGRLPV QRRNY GVV+A+TR++K+EG+
Sbjct: 133 SFGMKAVLGMTAGGIGSFVGTPAEIALIRMTGDGRLPVEQRRNYTGVVNALTRITKEEGV 192

Query: 186 TSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFVAAVAS 245
            +LWRG   TV RAM+V AAQLA+Y Q K+ +L  G ++DG+  H  AS  +G    +AS
Sbjct: 193 LTLWRGCTPTVLRAMVVNAAQLATYSQAKQALLASGKVQDGIFCHFLASMISGLATTIAS 252

Query: 246 NPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGPFTVVL 305
            PVD+ KTR+ +MKV  G+   Y  A D   K +K EG  AL+KGF P   R GP TV+ 
Sbjct: 253 MPVDIAKTRIQSMKVIDGK-PEYKNAFDVWGKVIKNEGIFALWKGFTPYYMRLGPHTVLT 296

Query: 306 FVTLEQVRKIFNQF 320
           F+ LEQ+   + Q+
Sbjct: 313 FIILEQMNAAYFQY 296

BLAST of CSPI02G21740 vs. ExPASy TrEMBL
Match: A0A5A7V9T0 (Mitochondrial uncoupling protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004130 PE=3 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 2.3e-171
Identity = 315/319 (98.75%), Postives = 316/319 (99.06%), Query Frame = 0

Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF 60
           MGFKGF EGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRS+VA ESF
Sbjct: 1   MGFKGFVEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSVVAQESF 60

Query: 61  HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP 120
           HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP
Sbjct: 61  HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP 120

Query: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180
           DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS
Sbjct: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180

Query: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240
           KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV
Sbjct: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240

Query: 241 AAVASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300
           AAVASNPVDVIKTRVMNMKVEAG TAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP
Sbjct: 241 AAVASNPVDVIKTRVMNMKVEAGATAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300

Query: 301 FTVVLFVTLEQVRKIFNQF 320
           FTVVLFVTLEQVRKIFNQF
Sbjct: 301 FTVVLFVTLEQVRKIFNQF 319

BLAST of CSPI02G21740 vs. ExPASy TrEMBL
Match: A0A0A0LLU3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G371610 PE=3 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 2.3e-171
Identity = 315/318 (99.06%), Postives = 316/318 (99.37%), Query Frame = 0

Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF 60
           MG KGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF
Sbjct: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF 60

Query: 61  HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP 120
           HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWS+P
Sbjct: 61  HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSNP 120

Query: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180
           DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS
Sbjct: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180

Query: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240
           KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV
Sbjct: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240

Query: 241 AAVASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300
           AAVASNPVDVIKTRVMNMKVEAGE APYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP
Sbjct: 241 AAVASNPVDVIKTRVMNMKVEAGEAAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300

Query: 301 FTVVLFVTLEQVRKIFNQ 319
           FTVVLFVTLEQVRKIFNQ
Sbjct: 301 FTVVLFVTLEQVRKIFNQ 318

BLAST of CSPI02G21740 vs. ExPASy TrEMBL
Match: A0A0A0LPN1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G371110 PE=3 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 9.1e-168
Identity = 308/319 (96.55%), Postives = 314/319 (98.43%), Query Frame = 0

Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF 60
           MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQL GEKP LPNL PALAFNASRS+VAP+ +
Sbjct: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLAGEKPALPNLPPALAFNASRSVVAPDYY 60

Query: 61  HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP 120
           HIPPPQPPRVGPISVG+RIVQSEGV+ALFSGVSATVLRQTLYSTTRMGLYDILKT+WSDP
Sbjct: 61  HIPPPQPPRVGPISVGMRIVQSEGVSALFSGVSATVLRQTLYSTTRMGLYDILKTRWSDP 120

Query: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180
           DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS
Sbjct: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180

Query: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240
           KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV
Sbjct: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240

Query: 241 AAVASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300
           AAVASNPVDVIKTRVMNMKVEAGE APYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP
Sbjct: 241 AAVASNPVDVIKTRVMNMKVEAGEAAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300

Query: 301 FTVVLFVTLEQVRKIFNQF 320
           FTVVLFVTLEQVRKIFNQF
Sbjct: 301 FTVVLFVTLEQVRKIFNQF 319

BLAST of CSPI02G21740 vs. ExPASy TrEMBL
Match: A0A5A7VA82 (Mitochondrial uncoupling protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004140 PE=3 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 8.5e-166
Identity = 306/319 (95.92%), Postives = 309/319 (96.87%), Query Frame = 0

Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF 60
           MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQL GEKP LPNLRPAL FN SRS+V PE F
Sbjct: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLAGEKPALPNLRPALHFNVSRSVVVPEYF 60

Query: 61  HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP 120
           H PP QPPRVGPIS+G+RIVQSEGV ALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP
Sbjct: 61  HPPPTQPPRVGPISIGMRIVQSEGVPALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP 120

Query: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180
           DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS
Sbjct: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180

Query: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240
           KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV
Sbjct: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240

Query: 241 AAVASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300
           AAVASNPVDVIKTRVMNMKVEAG TAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP
Sbjct: 241 AAVASNPVDVIKTRVMNMKVEAGATAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300

Query: 301 FTVVLFVTLEQVRKIFNQF 320
           FTVVLFVTLEQVRKIFNQF
Sbjct: 301 FTVVLFVTLEQVRKIFNQF 319

BLAST of CSPI02G21740 vs. ExPASy TrEMBL
Match: A0A6J1KQ28 (mitochondrial uncoupling protein 5-like OS=Cucurbita maxima OX=3661 GN=LOC111496577 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 1.4e-165
Identity = 305/321 (95.02%), Postives = 313/321 (97.51%), Query Frame = 0

Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRS--LVAPE 60
           MG KGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLP+LRPA+AFNASRS  +V PE
Sbjct: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPSLRPAVAFNASRSAAVVTPE 60

Query: 61  SFHIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWS 120
            FHIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYD+LKTKWS
Sbjct: 61  YFHIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWS 120

Query: 121 DPDSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITR 180
           DP+SG+MPL+RKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITR
Sbjct: 121 DPNSGNMPLSRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITR 180

Query: 181 MSKQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAG 240
           MSKQEGITSLWRGS+LTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASF AG
Sbjct: 181 MSKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFTAG 240

Query: 241 FVAAVASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQ 300
           FVAAVASNPVDVIKTRVMNMKVEAG  APYSGALDCAMKTVKAEGPMALYKGFIPTISRQ
Sbjct: 241 FVAAVASNPVDVIKTRVMNMKVEAGAAAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQ 300

Query: 301 GPFTVVLFVTLEQVRKIFNQF 320
           GPFTVVLFVTLEQVRKIFNQF
Sbjct: 301 GPFTVVLFVTLEQVRKIFNQF 321

BLAST of CSPI02G21740 vs. NCBI nr
Match: KAA0065082.1 (mitochondrial uncoupling protein 5 [Cucumis melo var. makuwa])

HSP 1 Score: 611.3 bits (1575), Expect = 4.8e-171
Identity = 315/319 (98.75%), Postives = 316/319 (99.06%), Query Frame = 0

Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF 60
           MGFKGF EGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRS+VA ESF
Sbjct: 1   MGFKGFVEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSVVAQESF 60

Query: 61  HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP 120
           HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP
Sbjct: 61  HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP 120

Query: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180
           DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS
Sbjct: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180

Query: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240
           KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV
Sbjct: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240

Query: 241 AAVASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300
           AAVASNPVDVIKTRVMNMKVEAG TAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP
Sbjct: 241 AAVASNPVDVIKTRVMNMKVEAGATAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300

Query: 301 FTVVLFVTLEQVRKIFNQF 320
           FTVVLFVTLEQVRKIFNQF
Sbjct: 301 FTVVLFVTLEQVRKIFNQF 319

BLAST of CSPI02G21740 vs. NCBI nr
Match: XP_031736495.1 (mitochondrial uncoupling protein 5 [Cucumis sativus] >XP_031736496.1 mitochondrial uncoupling protein 5 [Cucumis sativus] >KAE8652145.1 hypothetical protein Csa_022491 [Cucumis sativus])

HSP 1 Score: 610.1 bits (1572), Expect = 1.1e-170
Identity = 314/318 (98.74%), Postives = 316/318 (99.37%), Query Frame = 0

Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF 60
           MG KGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF
Sbjct: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF 60

Query: 61  HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP 120
           HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWS+P
Sbjct: 61  HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSNP 120

Query: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180
           DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPV+QRRNYAGVVDAITRMS
Sbjct: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVSQRRNYAGVVDAITRMS 180

Query: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240
           KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV
Sbjct: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240

Query: 241 AAVASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300
           AAVASNPVDVIKTRVMNMKVEAGE APYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP
Sbjct: 241 AAVASNPVDVIKTRVMNMKVEAGEAAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300

Query: 301 FTVVLFVTLEQVRKIFNQ 319
           FTVVLFVTLEQVRKIFNQ
Sbjct: 301 FTVVLFVTLEQVRKIFNQ 318

BLAST of CSPI02G21740 vs. NCBI nr
Match: XP_038884031.1 (mitochondrial uncoupling protein 5-like [Benincasa hispida])

HSP 1 Score: 603.6 bits (1555), Expect = 9.9e-169
Identity = 309/319 (96.87%), Postives = 314/319 (98.43%), Query Frame = 0

Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF 60
           MGFKGF EGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRS+VAPESF
Sbjct: 1   MGFKGFVEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSVVAPESF 60

Query: 61  HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP 120
           HIPPPQPPRVGPISVGVRIVQSEGV ALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP
Sbjct: 61  HIPPPQPPRVGPISVGVRIVQSEGVTALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP 120

Query: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180
           +SGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLP+AQRRNYAGVVDAITRMS
Sbjct: 121 NSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPMAQRRNYAGVVDAITRMS 180

Query: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240
           KQEGITSLWRGS+LTVNRAMIVTAAQLASYDQ+KETILEKGVMKDGLGTHVTASFAAGFV
Sbjct: 181 KQEGITSLWRGSSLTVNRAMIVTAAQLASYDQMKETILEKGVMKDGLGTHVTASFAAGFV 240

Query: 241 AAVASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300
           AAVASNPVDVIKTRVMNMKVEAG   PYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP
Sbjct: 241 AAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300

Query: 301 FTVVLFVTLEQVRKIFNQF 320
           FTVVLFVTLEQVRKIFNQF
Sbjct: 301 FTVVLFVTLEQVRKIFNQF 319

BLAST of CSPI02G21740 vs. NCBI nr
Match: XP_004148417.2 (mitochondrial uncoupling protein 5 [Cucumis sativus])

HSP 1 Score: 599.4 bits (1544), Expect = 1.9e-167
Identity = 308/319 (96.55%), Postives = 314/319 (98.43%), Query Frame = 0

Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF 60
           MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQL GEKP LPNL PALAFNASRS+VAP+ +
Sbjct: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLAGEKPALPNLPPALAFNASRSVVAPDYY 60

Query: 61  HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP 120
           HIPPPQPPRVGPISVG+RIVQSEGV+ALFSGVSATVLRQTLYSTTRMGLYDILKT+WSDP
Sbjct: 61  HIPPPQPPRVGPISVGMRIVQSEGVSALFSGVSATVLRQTLYSTTRMGLYDILKTRWSDP 120

Query: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180
           DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS
Sbjct: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180

Query: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240
           KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV
Sbjct: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240

Query: 241 AAVASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300
           AAVASNPVDVIKTRVMNMKVEAGE APYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP
Sbjct: 241 AAVASNPVDVIKTRVMNMKVEAGEAAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300

Query: 301 FTVVLFVTLEQVRKIFNQF 320
           FTVVLFVTLEQVRKIFNQF
Sbjct: 301 FTVVLFVTLEQVRKIFNQF 319

BLAST of CSPI02G21740 vs. NCBI nr
Match: KAA0065083.1 (mitochondrial uncoupling protein 5 [Cucumis melo var. makuwa])

HSP 1 Score: 592.8 bits (1527), Expect = 1.8e-165
Identity = 306/319 (95.92%), Postives = 309/319 (96.87%), Query Frame = 0

Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF 60
           MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQL GEKP LPNLRPAL FN SRS+V PE F
Sbjct: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLAGEKPALPNLRPALHFNVSRSVVVPEYF 60

Query: 61  HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP 120
           H PP QPPRVGPIS+G+RIVQSEGV ALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP
Sbjct: 61  HPPPTQPPRVGPISIGMRIVQSEGVPALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP 120

Query: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180
           DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS
Sbjct: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180

Query: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240
           KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV
Sbjct: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240

Query: 241 AAVASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300
           AAVASNPVDVIKTRVMNMKVEAG TAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP
Sbjct: 241 AAVASNPVDVIKTRVMNMKVEAGATAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300

Query: 301 FTVVLFVTLEQVRKIFNQF 320
           FTVVLFVTLEQVRKIFNQF
Sbjct: 301 FTVVLFVTLEQVRKIFNQF 319

BLAST of CSPI02G21740 vs. TAIR 10
Match: AT2G22500.1 (uncoupling protein 5 )

HSP 1 Score: 462.6 bits (1189), Expect = 2.5e-130
Identity = 237/320 (74.06%), Postives = 273/320 (85.31%), Query Frame = 0

Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPL-PNLRPALAFNASRSLVAPES 60
           MG KGFAEGGIASIVAGCSTHPLDLIKVRMQL GE  P+  NLRPALAF  S ++ AP  
Sbjct: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLQGESAPIQTNLRPALAFQTSTTVNAP-- 60

Query: 61  FHIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSD 120
                  P RVG I VG R+++ EG+ ALFSGVSATVLRQTLYSTTRMGLYDI+K +W+D
Sbjct: 61  -------PLRVGVIGVGSRLIREEGMRALFSGVSATVLRQTLYSTTRMGLYDIIKGEWTD 120

Query: 121 PDSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRM 180
           P++ +MPL +KI AG IAG IGAAVGNPADVAMVRMQADGRLP+  RRNY  V+DAIT+M
Sbjct: 121 PETKTMPLMKKIGAGAIAGAIGAAVGNPADVAMVRMQADGRLPLTDRRNYKSVLDAITQM 180

Query: 181 SKQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGF 240
            + EG+TSLWRGS+LT+NRAM+VT++QLASYD +KETILEKG++KDGLGTHV+ASFAAGF
Sbjct: 181 IRGEGVTSLWRGSSLTINRAMLVTSSQLASYDSVKETILEKGLLKDGLGTHVSASFAAGF 240

Query: 241 VAAVASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQG 300
           VA+VASNPVDVIKTRVMNMKV AG   PY GA+DCA+KTVKAEG M+LYKGFIPT+SRQ 
Sbjct: 241 VASVASNPVDVIKTRVMNMKVVAGVAPPYKGAVDCALKTVKAEGIMSLYKGFIPTVSRQA 300

Query: 301 PFTVVLFVTLEQVRKIFNQF 320
           PFTVVLFVTLEQV+K+F  +
Sbjct: 301 PFTVVLFVTLEQVKKLFKDY 311

BLAST of CSPI02G21740 vs. TAIR 10
Match: AT4G24570.1 (dicarboxylate carrier 2 )

HSP 1 Score: 456.8 bits (1174), Expect = 1.4e-128
Identity = 242/322 (75.16%), Postives = 267/322 (82.92%), Query Frame = 0

Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPN---LRPALAFNASRSLVAP 60
           MG K F EGGIAS++AGCSTHPLDLIKVR+QL GE P       LRPALAF  S    +P
Sbjct: 1   MGVKSFVEGGIASVIAGCSTHPLDLIKVRLQLHGEAPSTTTVTLLRPALAFPNS----SP 60

Query: 61  ESFHIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKW 120
            +F       P+VGPIS+G+ IV+SEG AALFSGVSAT+LRQTLYSTTRMGLY++LK KW
Sbjct: 61  AAFLETTSSVPKVGPISLGINIVKSEGAAALFSGVSATLLRQTLYSTTRMGLYEVLKNKW 120

Query: 121 SDPDSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAIT 180
           +DP+SG + L+RKI AGL+AGGIGAAVGNPADVAMVRMQADGRLP+AQRRNYAGV DAI 
Sbjct: 121 TDPESGKLNLSRKIGAGLVAGGIGAAVGNPADVAMVRMQADGRLPLAQRRNYAGVGDAIR 180

Query: 181 RMSKQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAA 240
            M K EG+TSLWRGSALT+NRAMIVTAAQLASYDQ KE ILE GVM DGLGTHV ASFAA
Sbjct: 181 SMVKGEGVTSLWRGSALTINRAMIVTAAQLASYDQFKEGILENGVMNDGLGTHVVASFAA 240

Query: 241 GFVAAVASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTVKAEGPMALYKGFIPTISR 300
           GFVA+VASNPVDVIKTRVMNMKV A     Y GA DCA+KTVKAEG MALYKGF+PT+ R
Sbjct: 241 GFVASVASNPVDVIKTRVMNMKVGA-----YDGAWDCAVKTVKAEGAMALYKGFVPTVCR 300

Query: 301 QGPFTVVLFVTLEQVRKIFNQF 320
           QGPFTVVLFVTLEQVRK+   F
Sbjct: 301 QGPFTVVLFVTLEQVRKLLRDF 313

BLAST of CSPI02G21740 vs. TAIR 10
Match: AT5G09470.1 (dicarboxylate carrier 3 )

HSP 1 Score: 366.3 bits (939), Expect = 2.5e-101
Identity = 200/334 (59.88%), Postives = 241/334 (72.16%), Query Frame = 0

Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGE------KPPLPNL-----------R 60
           MGFK F EGGIA+I+AG  THPLDLIKVRMQL GE      + P PNL           R
Sbjct: 1   MGFKPFLEGGIAAIIAGALTHPLDLIKVRMQLQGEHSFSLDQNPNPNLSLDHNLPVKPYR 60

Query: 61  PALAFNASRSLVAPESFHIPPPQPPR---VGPISVGVRIVQSEGVAALFSGVSATVLRQT 120
           P  A ++    ++    HI  P       + P +VG  IV++EG AALFSGVSAT+LRQ 
Sbjct: 61  PVFALDSLIGSISLLPLHIHAPSSSTRSVMTPFAVGAHIVKTEGPAALFSGVSATILRQM 120

Query: 121 LYSTTRMGLYDILKTKWSDPDSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGR 180
           LYS TRMG+YD LK +W+D  +G+ PL  KITAGLIAG +G+ VGNPADVAMVRMQADG 
Sbjct: 121 LYSATRMGIYDFLKRRWTDQLTGNFPLVTKITAGLIAGAVGSVVGNPADVAMVRMQADGS 180

Query: 181 LPVAQRRNYAGVVDAITRMSKQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEK 240
           LP+ +RRNY  VVDAI R+++QEG++SLWRGS LTVNRAMIVTA+QLA+YD +KE ++  
Sbjct: 181 LPLNRRRNYKSVVDAIDRIARQEGVSSLWRGSWLTVNRAMIVTASQLATYDHVKEILVAG 240

Query: 241 G-VMKDGLGTHVTASFAAGFVAAVASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTV 300
           G     G+GTHV ASFAAG VAAVASNP+DV+KTR+MN   E      Y G LDCA+K V
Sbjct: 241 GRGTPGGIGTHVAASFAAGIVAAVASNPIDVVKTRMMNADKEI-----YGGPLDCAVKMV 300

Query: 301 KAEGPMALYKGFIPTISRQGPFTVVLFVTLEQVR 314
             EGPMALYKG +PT +RQGPFT++LF+TLEQVR
Sbjct: 301 AEEGPMALYKGLVPTATRQGPFTMILFLTLEQVR 329

BLAST of CSPI02G21740 vs. TAIR 10
Match: AT3G54110.1 (plant uncoupling mitochondrial protein 1 )

HSP 1 Score: 206.5 bits (524), Expect = 3.3e-53
Identity = 120/312 (38.46%), Postives = 176/312 (56.41%), Query Frame = 0

Query: 4   KGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESFHIP 63
           K FA    A+ V    T PLD  KVR+QL          + ALA + +            
Sbjct: 13  KTFACSAFAACVGEVCTIPLDTAKVRLQLQ---------KSALAGDVT------------ 72

Query: 64  PPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDPD-S 123
              P   G +     I + EG+ +L+ GV   + RQ L+   R+G+Y+ +K  +   D  
Sbjct: 73  --LPKYRGLLGTVGTIAREEGLRSLWKGVVPGLHRQCLFGGLRIGMYEPVKNLYVGKDFV 132

Query: 124 GSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMSKQ 183
           G +PL++KI AGL  G +G  V NP D+  VR+QA+G+L     R Y+G ++A + + +Q
Sbjct: 133 GDVPLSKKILAGLTTGALGIMVANPTDLVKVRLQAEGKLAAGAPRRYSGALNAYSTIVRQ 192

Query: 184 EGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFVAA 243
           EG+ +LW G    V R  I+ AA+LASYDQ+KETIL+     D + TH+ +   AGF A 
Sbjct: 193 EGVRALWTGLGPNVARNAIINAAELASYDQVKETILKIPGFTDNVVTHILSGLGAGFFAV 252

Query: 244 VASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGPFT 303
              +PVDV+K+R+M      G++  Y G +DC +KT+K++GPMA YKGFIP   R G + 
Sbjct: 253 CIGSPVDVVKSRMM------GDSGAYKGTIDCFVKTLKSDGPMAFYKGFIPNFGRLGSWN 295

Query: 304 VVLFVTLEQVRK 315
           V++F+TLEQ +K
Sbjct: 313 VIMFLTLEQAKK 295

BLAST of CSPI02G21740 vs. TAIR 10
Match: AT5G58970.1 (uncoupling protein 2 )

HSP 1 Score: 206.1 bits (523), Expect = 4.3e-53
Identity = 124/316 (39.24%), Postives = 175/316 (55.38%), Query Frame = 0

Query: 6   FAEGGIASIVAGC----STHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESFH 65
           F E  I S  A C     T PLD  KVR+QL                   R +   +  +
Sbjct: 12  FLETFICSAFAACFAELCTIPLDTAKVRLQL------------------QRKIPTGDGEN 71

Query: 66  IPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDPD 125
           +P  +    G I     I + EG++ L+ GV A + RQ +Y   R+GLY+ +KT     D
Sbjct: 72  LPKYR----GSIGTLATIAREEGISGLWKGVIAGLHRQCIYGGLRIGLYEPVKTLLVGSD 131

Query: 126 -SGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 185
             G +PL +KI A L+ G I   V NP D+  VR+Q++G+LP    R YAG VDA   + 
Sbjct: 132 FIGDIPLYQKILAALLTGAIAIIVANPTDLVKVRLQSEGKLPAGVPRRYAGAVDAYFTIV 191

Query: 186 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 245
           K EG+++LW G    + R  IV AA+LASYDQIKETI++    +D + TH+ A  AAGF 
Sbjct: 192 KLEGVSALWTGLGPNIARNAIVNAAELASYDQIKETIMKIPFFRDSVLTHLLAGLAAGFF 251

Query: 246 AAVASNPVDVIKTRVMNMKVEAGETAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 305
           A    +P+DV+K+R+M         + Y   +DC +KT+K EG MA YKGF+P  +R G 
Sbjct: 252 AVCIGSPIDVVKSRMMG-------DSTYRNTVDCFIKTMKTEGIMAFYKGFLPNFTRLGT 298

Query: 306 FTVVLFVTLEQVRKIF 317
           +  ++F+TLEQV+K+F
Sbjct: 312 WNAIMFLTLEQVKKVF 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SJY53.6e-12974.06Mitochondrial uncoupling protein 5 OS=Arabidopsis thaliana OX=3702 GN=PUMP5 PE=2... [more]
Q9SB522.0e-12775.16Mitochondrial uncoupling protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUMP4 PE=2... [more]
Q9FY683.5e-10059.88Mitochondrial uncoupling protein 6 OS=Arabidopsis thaliana OX=3702 GN=PUMP6 PE=2... [more]
Q54PY71.4e-6443.49Probable mitochondrial 2-oxoglutarate/malate carrier protein OS=Dictyostelium di... [more]
P909926.7e-5942.04Mitochondrial 2-oxoglutarate/malate carrier protein OS=Caenorhabditis elegans OX... [more]
Match NameE-valueIdentityDescription
A0A5A7V9T02.3e-17198.75Mitochondrial uncoupling protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A0A0LLU32.3e-17199.06Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G371610 PE=3 SV=1[more]
A0A0A0LPN19.1e-16896.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G371110 PE=3 SV=1[more]
A0A5A7VA828.5e-16695.92Mitochondrial uncoupling protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A6J1KQ281.4e-16595.02mitochondrial uncoupling protein 5-like OS=Cucurbita maxima OX=3661 GN=LOC111496... [more]
Match NameE-valueIdentityDescription
KAA0065082.14.8e-17198.75mitochondrial uncoupling protein 5 [Cucumis melo var. makuwa][more]
XP_031736495.11.1e-17098.74mitochondrial uncoupling protein 5 [Cucumis sativus] >XP_031736496.1 mitochondri... [more]
XP_038884031.19.9e-16996.87mitochondrial uncoupling protein 5-like [Benincasa hispida][more]
XP_004148417.21.9e-16796.55mitochondrial uncoupling protein 5 [Cucumis sativus][more]
KAA0065083.11.8e-16595.92mitochondrial uncoupling protein 5 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT2G22500.12.5e-13074.06uncoupling protein 5 [more]
AT4G24570.11.4e-12875.16dicarboxylate carrier 2 [more]
AT5G09470.12.5e-10159.88dicarboxylate carrier 3 [more]
AT3G54110.13.3e-5338.46plant uncoupling mitochondrial protein 1 [more]
AT5G58970.14.3e-5339.24uncoupling protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002067Mitochondrial carrier proteinPRINTSPR00926MITOCARRIERcoord: 5..18
score: 30.68
coord: 18..32
score: 24.26
coord: 140..158
score: 25.19
coord: 234..256
score: 28.65
IPR018108Mitochondrial substrate/solute carrierPFAMPF00153Mito_carrcoord: 4..116
e-value: 4.5E-16
score: 58.5
coord: 228..318
e-value: 1.5E-19
score: 69.7
coord: 125..219
e-value: 1.4E-18
score: 66.6
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 225..315
score: 26.160038
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 4..116
score: 17.66461
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 125..216
score: 26.5891
IPR023395Mitochondrial carrier domain superfamilyGENE3D1.50.40.10Mitochondrial carrier domaincoord: 4..318
e-value: 1.8E-83
score: 281.8
IPR023395Mitochondrial carrier domain superfamilySUPERFAMILY103506Mitochondrial carriercoord: 4..312
NoneNo IPR availablePANTHERPTHR45618:SF45MITOCHONDRIAL UNCOUPLING PROTEIN 5coord: 1..319
NoneNo IPR availablePANTHERPTHR45618MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATEDcoord: 1..319

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G21740.2CSPI02G21740.2mRNA
CSPI02G21740.1CSPI02G21740.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071423 malate transmembrane transport
biological_process GO:0006839 mitochondrial transport
biological_process GO:1902356 oxaloacetate(2-) transmembrane transport
biological_process GO:0035435 phosphate ion transmembrane transport
biological_process GO:0071422 succinate transmembrane transport
biological_process GO:1902358 sulfate transmembrane transport
biological_process GO:0015709 thiosulfate transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0031966 mitochondrial membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0015140 malate transmembrane transporter activity
molecular_function GO:0015131 oxaloacetate transmembrane transporter activity
molecular_function GO:0015141 succinate transmembrane transporter activity
molecular_function GO:0015116 sulfate transmembrane transporter activity
molecular_function GO:0015117 thiosulfate transmembrane transporter activity