CSPI02G21320 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G21320
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionpurine permease 3
LocationChr2: 18697147 .. 18698685 (+)
RNA-Seq ExpressionCSPI02G21320
SyntenyCSPI02G21320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCTCCTTGAGTCATTCAGCCTCAAAAATTAACAGACTCTTTCCTTCCGGCGAAAGCGATGGACAAAGAACAGCAACAACAAAACACCCCCTCCGTGAAACGAACACTTCTAATTTTCAATTGCTGTTTATTGGCCGTCGGTAACTGTGGCGGTCCTCTGATTATGCGTTTGTATTTTGTCCACGGCGGAAAGCGTGTTTGGCTCTCTAGCTGTCTAGAAACCGGCGGATGGCCAATTATTTTCATCCCACTTCTAATCAGTTACATCCATCGCCGCCGTCTCGCCGCCTTGGACCCATCGGGATCTCCCAACTCCGCCCAGTTCATCTTCATGAAACCGCGGCTATTTTTAGCCTCCGCTTTCATCGGCATCCTCACCGGCTTCGACGACTACTTATACGCTTACGGCGTCGCTCGACTACCGGTTTCCACGTCGGCTTTAATCATTGCCTGCCAGCTGGCTTTCACAGCGGGATTCGCGTTCTTATTGGTCAAACAGAAATTCACATCCTATTCTATAAACGCCGTCGTTTTGCTTACTATTGGAGGCGCAGTTTTGGCATTACACACAAGCGGTGACCGTCCTGCCGGAGAATCCAATAAAGATTACATCGCTGGGTTTCTAATGACGGTTGCCGCCGCGGTTGTCTACGGATTCGTTCTGCCGCTTGTTGAATTAACATACAAGAAAGCTCGACAACAAATCACTTACACTTTAGTTCTTGAGTTTCAGTTGATTATGTCTTTGTTCGCCACGATTGTATGCGCCATTGGCATGTTAATCAACAACGATTTCCAGGTAAATTTTAAAGATAATCATACACCCGTTTGATAAAATTTTTGTTTATAGTTGATTGAATTAGTTTTTTCTTTTTGATCTATACAGGTAATCCCAAGGGAGGCAGAAACATTTGGATTGGGAAAGTTCAGATACTATTTGGTATTAGTATTAAGCGCCATACTTTGGCAGGGTTTCTTCTTAGGAGCTATTGGAGTTATTTTTAGCTCATCGTCTCTATTTTCAGGAATAGTAATTGCTGTTCTCTTGCCAGTCACTGAAATCCTTGCAGTTATCATTTTCAATGAGAGATTTCAAGCAGAAAAGGGTGTTTCTTTGGCACTAAATCTTTGGGGATTTTTGTCTTATTTTTACGGTGAAATTAAGCACAACAAAAGGAAGAAGCTAGAGCTCCAAAGATATGAAGAAACAACGAGTACTCAAGTTGCAAATGTCTAAATTAAACAACTTTTTTTTTTTTTTTTATTGATTTTAAATTTAAGATTGTTGTTGTATTGTTGTATTGTGAGATGAAGAGTAATATGGTGTAAGTTTGTGTTTAAGGGAATGGATGTATATGGTGTGTGTTAAGGATTTTGATATAGGTGGGTGGATATAAAGTTACTGTGGAAACATCCCATTCCCTTTTCTTTTCTTTTTTTCTTTTTAATTATTAATTGTCAAAAAAAGGTTTTAGAAGAATAATATATTTAAGTAGGGAGATATTTTTATTGCTTAATTTATTCGACTTTTAATACCCA

mRNA sequence

ATCTCCTTGAGTCATTCAGCCTCAAAAATTAACAGACTCTTTCCTTCCGGCGAAAGCGATGGACAAAGAACAGCAACAACAAAACACCCCCTCCGTGAAACGAACACTTCTAATTTTCAATTGCTGTTTATTGGCCGTCGGTAACTGTGGCGGTCCTCTGATTATGCGTTTGTATTTTGTCCACGGCGGAAAGCGTGTTTGGCTCTCTAGCTGTCTAGAAACCGGCGGATGGCCAATTATTTTCATCCCACTTCTAATCAGTTACATCCATCGCCGCCGTCTCGCCGCCTTGGACCCATCGGGATCTCCCAACTCCGCCCAGTTCATCTTCATGAAACCGCGGCTATTTTTAGCCTCCGCTTTCATCGGCATCCTCACCGGCTTCGACGACTACTTATACGCTTACGGCGTCGCTCGACTACCGGTTTCCACGTCGGCTTTAATCATTGCCTGCCAGCTGGCTTTCACAGCGGGATTCGCGTTCTTATTGGTCAAACAGAAATTCACATCCTATTCTATAAACGCCGTCGTTTTGCTTACTATTGGAGGCGCAGTTTTGGCATTACACACAAGCGGTGACCGTCCTGCCGGAGAATCCAATAAAGATTACATCGCTGGGTTTCTAATGACGGTTGCCGCCGCGGTTGTCTACGGATTCGTTCTGCCGCTTGTTGAATTAACATACAAGAAAGCTCGACAACAAATCACTTACACTTTAGTTCTTGAGTTTCAGTTGATTATGTCTTTGTTCGCCACGATTGTATGCGCCATTGGCATGTTAATCAACAACGATTTCCAGGTAATCCCAAGGGAGGCAGAAACATTTGGATTGGGAAAGTTCAGATACTATTTGGTATTAGTATTAAGCGCCATACTTTGGCAGGGTTTCTTCTTAGGAGCTATTGGAGTTATTTTTAGCTCATCGTCTCTATTTTCAGGAATAGTAATTGCTGTTCTCTTGCCAGTCACTGAAATCCTTGCAGTTATCATTTTCAATGAGAGATTTCAAGCAGAAAAGGGTGTTTCTTTGGCACTAAATCTTTGGGGATTTTTGTCTTATTTTTACGGTGAAATTAAGCACAACAAAAGGAAGAAGCTAGAGCTCCAAAGATATGAAGAAACAACGAGTACTCAAGTTGCAAATGTCTAAATTAAACAACTTTTTTTTTTTTTTTTATTGATTTTAAATTTAAGATTGTTGTTGTATTGTTGTATTGTGAGATGAAGAGTAATATGGTGTAAGTTTGTGTTTAAGGGAATGGATGTATATGGTGTGTGTTAAGGATTTTGATATAGGTGGGTGGATATAAAGTTACTGTGGAAACATCCCATTCCCTTTTCTTTTCTTTTTTTCTTTTTAATTATTAATTGTCAAAAAAAGGTTTTAGAAGAATAATATATTTAAGTAGGGAGATATTTTTATTGCTTAATTTATTCGACTTTTAATACCCA

Coding sequence (CDS)

ATGGACAAAGAACAGCAACAACAAAACACCCCCTCCGTGAAACGAACACTTCTAATTTTCAATTGCTGTTTATTGGCCGTCGGTAACTGTGGCGGTCCTCTGATTATGCGTTTGTATTTTGTCCACGGCGGAAAGCGTGTTTGGCTCTCTAGCTGTCTAGAAACCGGCGGATGGCCAATTATTTTCATCCCACTTCTAATCAGTTACATCCATCGCCGCCGTCTCGCCGCCTTGGACCCATCGGGATCTCCCAACTCCGCCCAGTTCATCTTCATGAAACCGCGGCTATTTTTAGCCTCCGCTTTCATCGGCATCCTCACCGGCTTCGACGACTACTTATACGCTTACGGCGTCGCTCGACTACCGGTTTCCACGTCGGCTTTAATCATTGCCTGCCAGCTGGCTTTCACAGCGGGATTCGCGTTCTTATTGGTCAAACAGAAATTCACATCCTATTCTATAAACGCCGTCGTTTTGCTTACTATTGGAGGCGCAGTTTTGGCATTACACACAAGCGGTGACCGTCCTGCCGGAGAATCCAATAAAGATTACATCGCTGGGTTTCTAATGACGGTTGCCGCCGCGGTTGTCTACGGATTCGTTCTGCCGCTTGTTGAATTAACATACAAGAAAGCTCGACAACAAATCACTTACACTTTAGTTCTTGAGTTTCAGTTGATTATGTCTTTGTTCGCCACGATTGTATGCGCCATTGGCATGTTAATCAACAACGATTTCCAGGTAATCCCAAGGGAGGCAGAAACATTTGGATTGGGAAAGTTCAGATACTATTTGGTATTAGTATTAAGCGCCATACTTTGGCAGGGTTTCTTCTTAGGAGCTATTGGAGTTATTTTTAGCTCATCGTCTCTATTTTCAGGAATAGTAATTGCTGTTCTCTTGCCAGTCACTGAAATCCTTGCAGTTATCATTTTCAATGAGAGATTTCAAGCAGAAAAGGGTGTTTCTTTGGCACTAAATCTTTGGGGATTTTTGTCTTATTTTTACGGTGAAATTAAGCACAACAAAAGGAAGAAGCTAGAGCTCCAAAGATATGAAGAAACAACGAGTACTCAAGTTGCAAATGTCTAA

Protein sequence

MDKEQQQQNTPSVKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPIIFIPLLISYIHRRRLAALDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVARLPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGESNKDYIAGFLMTVAAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGMLINNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVLLPVTEILAVIIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKRKKLELQRYEETTSTQVANV*
Homology
BLAST of CSPI02G21320 vs. ExPASy Swiss-Prot
Match: Q9FZ95 (Purine permease 3 OS=Arabidopsis thaliana OX=3702 GN=PUP3 PE=2 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 2.7e-117
Identity = 212/348 (60.92%), Postives = 279/348 (80.17%), Query Frame = 0

Query: 15  RTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPIIFIPLLISYIHRRR 74
           + L+I NC +LA+GNCGGPLIMRLYF +GGKR+W S+ LET G+P+IFIPLL SYI RRR
Sbjct: 3   KALVIINCIILAIGNCGGPLIMRLYFNNGGKRIWFSTFLETAGFPVIFIPLLFSYITRRR 62

Query: 75  LAALDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVARLPVSTSALIIACQL 134
                 +   +S  F  +KPRL +A+  +GIL+GFD+YLYAYG+A LPVST+ALIIA QL
Sbjct: 63  -----SNNVGDSTSFFLIKPRLLIAAVIVGILSGFDNYLYAYGIAYLPVSTAALIIASQL 122

Query: 135 AFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGESNKDYIAGFLMTVAA 194
           AF A F+F +VK KFT ++INAVVLLT+G AVL +HT  D+P  E++K YI GFL+TVAA
Sbjct: 123 AFIAIFSFFMVKHKFTPFTINAVVLLTVGAAVLGMHTETDKPVHETHKQYITGFLITVAA 182

Query: 195 AVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGMLINNDFQVIPREAE 254
           AV+Y F+LPLVEL Y+KA+Q ++YTLVLEFQLI+ L A+IV  IGM I  DF+ +P+EA 
Sbjct: 183 AVMYAFILPLVELAYQKAKQTMSYTLVLEFQLILCLLASIVSVIGMFIAGDFKALPKEAR 242

Query: 255 TFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVLLPVTEILAVIIFNE 314
            F LG+  +Y+V V SAI+WQGFFLGAIG+IFS+SSL SGI+I+VLLP+TE+LAVI ++E
Sbjct: 243 EFKLGEALFYVVAVFSAIIWQGFFLGAIGLIFSTSSLVSGIMISVLLPITEVLAVIFYHE 302

Query: 315 RFQAEKGVSLALNLWGFLSYFYGEIKHNK-RKKLELQRYEETTSTQVA 362
           +FQAEKG+SLAL+LWGF+SYFYGEIK  + +++++ +  +ET  + ++
Sbjct: 303 KFQAEKGLSLALSLWGFVSYFYGEIKSGEDKRRIQQEESQETEQSSLS 345

BLAST of CSPI02G21320 vs. ExPASy Swiss-Prot
Match: Q9FZ96 (Purine permease 1 OS=Arabidopsis thaliana OX=3702 GN=PUP1 PE=1 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 3.8e-111
Identity = 204/335 (60.90%), Postives = 260/335 (77.61%), Query Frame = 0

Query: 13  VKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPIIFIPLLISYIHR 72
           +K  L+I NC +L +G CGGPL+ RLYF +GGKR+W  S L T G+PII IPLL+S++ R
Sbjct: 1   MKNGLIIINCIILTIGTCGGPLLTRLYFTNGGKRIWFMSFLSTAGFPIILIPLLVSFLSR 60

Query: 73  RRLAALDPSGSPN--SAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVARLPVSTSALII 132
           RR +  +P+ + N    +   M+  LF+AS  IG+LTG D+YLY+YG+A LPVSTS+LII
Sbjct: 61  RR-SNRNPNNAENKRKTKLFLMETPLFIASIVIGLLTGLDNYLYSYGLAYLPVSTSSLII 120

Query: 133 ACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGESNKDYIAGFLM 192
             QLAF A FAFLLVKQKFT +SINAVVLLT+G  +LALH+ GD+PA ES K+Y+ GFLM
Sbjct: 121 GTQLAFNALFAFLLVKQKFTPFSINAVVLLTVGIGILALHSDGDKPAKESKKEYVVGFLM 180

Query: 193 TVAAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGMLINNDFQVIP 252
           TV AA++Y F+LPLVELTYKKARQ+IT+ LVLE Q++M L AT  C IGM I  DF+VI 
Sbjct: 181 TVVAALLYAFILPLVELTYKKARQEITFPLVLEIQMVMCLAATFFCVIGMFIVGDFKVIA 240

Query: 253 REAETFGL-GKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVLLPVTEILAV 312
           REA  F + G   YY ++V++ I+WQGFFLGAIG++F +SSL SG++I+VLLPVTE+ AV
Sbjct: 241 REAREFKIGGSVFYYALIVITGIIWQGFFLGAIGIVFCASSLASGVLISVLLPVTEVFAV 300

Query: 313 IIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKR 345
           + F E+FQAEKGVSL L+LWGF+SYFYGE K  K+
Sbjct: 301 VCFREKFQAEKGVSLLLSLWGFVSYFYGEFKSGKK 334

BLAST of CSPI02G21320 vs. ExPASy Swiss-Prot
Match: Q94GB1 (Purine permease 2 OS=Arabidopsis thaliana OX=3702 GN=PUP2 PE=1 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 2.9e-103
Identity = 190/347 (54.76%), Postives = 258/347 (74.35%), Query Frame = 0

Query: 13  VKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPIIFIPLLISYIHR 72
           +K  L+I NC  LA+GNCGGPL+MRLYF +GG+R+W  S L+T G P+IF PLL+S++ R
Sbjct: 3   MKTVLVIINCIFLAIGNCGGPLMMRLYFQNGGERIWFPSFLQTVGCPLIFFPLLLSFLRR 62

Query: 73  RRLAALDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVARLPVSTSALIIAC 132
           RR           +  F  MKP LF+A+  +G+L GFD+YLY+YG+A +PVST++LII+ 
Sbjct: 63  RRCLE-----EQETTPFFLMKPPLFIAAIVVGLLVGFDNYLYSYGLAYIPVSTASLIISA 122

Query: 133 QLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGESNKDYIAGFLMTV 192
           QL FTA FAF +VKQKFT ++INA+VLLT G  VLAL++  D+ A E++K+Y+ GF+MT+
Sbjct: 123 QLGFTALFAFFMVKQKFTPFTINAIVLLTGGAVVLALNSDSDKLANETHKEYVVGFIMTL 182

Query: 193 AAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGMLINNDF------ 252
            AA++YGF+LPLVEL+YKK+ Q+ITYTL LEFQ+++   AT VC +GML   DF      
Sbjct: 183 GAALLYGFILPLVELSYKKSGQRITYTLALEFQMVLCFAATCVCLVGMLAAGDFKVKHAL 242

Query: 253 -----QVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVLL 312
                +VI  EA  F LG+  YY+V+V +AI+WQ FF+GAIG+IF +SSL SGI+++ LL
Sbjct: 243 FIFKNRVIAGEARDFKLGESLYYVVIVFTAIIWQAFFVGAIGLIFCASSLVSGIMVSALL 302

Query: 313 PVTEILAVIIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKRKKLE 349
           PVT ILAVI F E+FQA KGV+LAL+LWG +SYFYG++K  ++ K +
Sbjct: 303 PVTVILAVICFQEKFQAGKGVALALSLWGSVSYFYGQVKSEEKTKAQ 344

BLAST of CSPI02G21320 vs. ExPASy Swiss-Prot
Match: Q8RY74 (Probable purine permease 22 OS=Arabidopsis thaliana OX=3702 GN=PUP22 PE=2 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 9.3e-49
Identity = 117/325 (36.00%), Postives = 185/325 (56.92%), Query Frame = 0

Query: 37  RLYFVHGGKRVWLSSCLETGGWPIIFIPLLISYIHRRRLAALDPSGSPNSAQFIFMKPRL 96
           RLY+ +GGK  ++ + L+  G+P++ +    S I + +    + S SP+           
Sbjct: 61  RLYYENGGKSTYVVTLLQLIGFPVLILFRFFSRIRQPKSTDTNFSQSPSFTT-------- 120

Query: 97  FLASAFI--GILTGFDDYLYAYGVARLPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSI 156
            LAS ++  G+L     YL A G+  LPVST +LI+A QLAFTA F++ L  QKFT   +
Sbjct: 121 -LASVYLCTGLLVSAYAYLSAVGLLYLPVSTFSLILASQLAFTAFFSYFLNSQKFTPLIV 180

Query: 157 NAVVLLTIGGAVLALHTSGDRPAGESNKDYIAGFLMTVAAAVVYGFVLPLVELTYKKARQ 216
           N++ LLT+  A+L ++T  +     S   Y+ GF+ T+ A+   G VL L++L ++K   
Sbjct: 181 NSLFLLTVSSALLVVNTDSENTTNVSRVQYVIGFICTIGASAGIGLVLSLIQLLFRKVFT 240

Query: 217 QITYTLVLEFQLIMSLFATIVCAIGMLINNDFQVIPREAETFGLGKFRYYLVLVLSAILW 276
           + T + VL+     SL AT V  IG+  + +++ +P E   + LGK  Y L L  +AI W
Sbjct: 241 KHTSSAVLDLANYQSLVATCVVLIGLFASGEWRTLPSEMRNYKLGKVSYILTLASAAIFW 300

Query: 277 QGFFLGAIGVIFSSSSLFSGIVIAVLLPVTEILAVIIFNERFQAEKGVSLALNLWGFLSY 336
           Q + +G +G+IF SSS+FS  + AV LP+  ++AVI+F+++  A K  S+ L +WGFLS+
Sbjct: 301 QVYTVGCVGLIFESSSVFSNSITAVGLPIVPVVAVIVFHDKMDASKIFSIILAIWGFLSF 360

Query: 337 FYGE-IKHNKRKKLELQRYEETTST 359
            Y   +   K K  + +  EE T T
Sbjct: 361 VYQHYLDEKKLKTCQTKPVEEETQT 376

BLAST of CSPI02G21320 vs. ExPASy Swiss-Prot
Match: Q9LPF6 (Probable purine permease 11 OS=Arabidopsis thaliana OX=3702 GN=PUP11 PE=1 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.2e-48
Identity = 108/320 (33.75%), Postives = 185/320 (57.81%), Query Frame = 0

Query: 17  LLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPIIFIPLLISYIHRRRLA 76
           L+  N   L  G     L+ R Y+  GG   W+++ ++T  +PI++IPLL+         
Sbjct: 45  LVSVNIFFLIGGQAASVLLGRFYYDEGGNSKWMATLVQTAAFPILYIPLLL--------- 104

Query: 77  ALDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVARLPVSTSALIIACQLAF 136
            L  S S  S++       + L    +G++   D+ LY+ G+  L  ST +LI A QLAF
Sbjct: 105 -LPSSASVESSESSCSLKYIVLIYVLLGVIIAGDNMLYSVGLLYLSASTYSLICATQLAF 164

Query: 137 TAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGESNKDYIAGFLMTVAAAV 196
            A F++ +  QKFT+  +N+VVLL+   A++AL+   D P+G S   YI GF+ T+AA+ 
Sbjct: 165 NAVFSYFINAQKFTALILNSVVLLSFSAALIALNDDADTPSGVSRSKYIVGFVCTLAASA 224

Query: 197 VYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGMLINNDFQVIPREAETF 256
           +Y  +L L++ +++K  ++ T+++VLE Q+  SL AT V  IG+  + +++ +  E E +
Sbjct: 225 LYSLLLSLMQFSFEKILKRETFSVVLEMQIYTSLVATCVSVIGLFASGEWRTLHGEMEGY 284

Query: 257 GLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVLLPVTEILAVIIFNERF 316
             G+  Y L LV +A+ WQ   +G +G+IF  +SLFS ++  + L VT + A+++F ++ 
Sbjct: 285 HKGQASYVLTLVWTAVTWQVCSVGVVGLIFLVTSLFSNVISTLSLAVTPLAALVVFRDKM 344

Query: 317 QAEKGVSLALNLWGFLSYFY 337
              K +++ + +WGF SY Y
Sbjct: 345 SGVKIMAMLIAIWGFASYVY 354

BLAST of CSPI02G21320 vs. ExPASy TrEMBL
Match: A0A0A0LPJ9 (Probable purine permease OS=Cucumis sativus OX=3659 GN=Csa_2G369730 PE=3 SV=1)

HSP 1 Score: 701.8 bits (1810), Expect = 1.5e-198
Identity = 362/363 (99.72%), Postives = 362/363 (99.72%), Query Frame = 0

Query: 1   MDKEQQQQNTPSVKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPI 60
           MDKEQQQQNTPSVKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPI
Sbjct: 1   MDKEQQQQNTPSVKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPI 60

Query: 61  IFIPLLISYIHRRRLAALDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVAR 120
           IFIPLLISYIHRRRLAALDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVAR
Sbjct: 61  IFIPLLISYIHRRRLAALDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVAR 120

Query: 121 LPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGES 180
           LPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGES
Sbjct: 121 LPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGES 180

Query: 181 NKDYIAGFLMTVAAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGM 240
           NKDYIAGFLMTVAAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGM
Sbjct: 181 NKDYIAGFLMTVAAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGM 240

Query: 241 LINNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVL 300
           LINNDFQVIPREAE FGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVL
Sbjct: 241 LINNDFQVIPREAEAFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVL 300

Query: 301 LPVTEILAVIIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKRKKLELQRYEETTSTQV 360
           LPVTEILAVIIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKRKKLELQRYEETTSTQV
Sbjct: 301 LPVTEILAVIIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKRKKLELQRYEETTSTQV 360

Query: 361 ANV 364
           ANV
Sbjct: 361 ANV 363

BLAST of CSPI02G21320 vs. ExPASy TrEMBL
Match: A0A5A7V9X1 (Probable purine permease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004570 PE=3 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 3.5e-184
Identity = 340/365 (93.15%), Postives = 351/365 (96.16%), Query Frame = 0

Query: 1   MDKEQQ--QQNTPSVKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGW 60
           MDKEQQ  QQNT +VKRTLLIFNC LLAVGNCGGPL+MRLYFVHGG RVWLSS LETGGW
Sbjct: 1   MDKEQQQHQQNTSAVKRTLLIFNCFLLAVGNCGGPLVMRLYFVHGGNRVWLSSFLETGGW 60

Query: 61  PIIFIPLLISYIHRRRLAALDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGV 120
           PIIFIPLLISYI RRRLAALDPSGS +S+Q IFMKPRLFLASA IGILTGFDDYLYAYGV
Sbjct: 61  PIIFIPLLISYIRRRRLAALDPSGSADSSQIIFMKPRLFLASAVIGILTGFDDYLYAYGV 120

Query: 121 ARLPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAG 180
           ARLPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAG
Sbjct: 121 ARLPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAG 180

Query: 181 ESNKDYIAGFLMTVAAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAI 240
           ESNKDYIAGFLMTVAAAVVYGF+LPLVELTYKKARQQITYTLVLE+QLIMSLFATIVC I
Sbjct: 181 ESNKDYIAGFLMTVAAAVVYGFILPLVELTYKKARQQITYTLVLEYQLIMSLFATIVCVI 240

Query: 241 GMLINNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIA 300
           GMLINNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIA
Sbjct: 241 GMLINNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIA 300

Query: 301 VLLPVTEILAVIIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKRKKLELQRYEETTST 360
           VLLPVTEILAVIIFNE+FQAEK VSLALNLWGF+SYFYGEIK+NK+KKLELQRYEET ST
Sbjct: 301 VLLPVTEILAVIIFNEKFQAEKAVSLALNLWGFVSYFYGEIKNNKKKKLELQRYEETAST 360

Query: 361 QVANV 364
           Q+ NV
Sbjct: 361 QITNV 365

BLAST of CSPI02G21320 vs. ExPASy TrEMBL
Match: A0A1S3BC92 (Probable purine permease OS=Cucumis melo OX=3656 GN=LOC103488091 PE=3 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 3.5e-184
Identity = 340/365 (93.15%), Postives = 351/365 (96.16%), Query Frame = 0

Query: 1   MDKEQQ--QQNTPSVKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGW 60
           MDKEQQ  QQNT +VKRTLLIFNC LLAVGNCGGPL+MRLYFVHGG RVWLSS LETGGW
Sbjct: 1   MDKEQQQHQQNTSAVKRTLLIFNCFLLAVGNCGGPLVMRLYFVHGGNRVWLSSFLETGGW 60

Query: 61  PIIFIPLLISYIHRRRLAALDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGV 120
           PIIFIPLLISYI RRRLAALDPSGS +S+Q IFMKPRLFLASA IGILTGFDDYLYAYGV
Sbjct: 61  PIIFIPLLISYIRRRRLAALDPSGSADSSQIIFMKPRLFLASAVIGILTGFDDYLYAYGV 120

Query: 121 ARLPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAG 180
           ARLPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAG
Sbjct: 121 ARLPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAG 180

Query: 181 ESNKDYIAGFLMTVAAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAI 240
           ESNKDYIAGFLMTVAAAVVYGF+LPLVELTYKKARQQITYTLVLE+QLIMSLFATIVC I
Sbjct: 181 ESNKDYIAGFLMTVAAAVVYGFILPLVELTYKKARQQITYTLVLEYQLIMSLFATIVCVI 240

Query: 241 GMLINNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIA 300
           GMLINNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIA
Sbjct: 241 GMLINNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIA 300

Query: 301 VLLPVTEILAVIIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKRKKLELQRYEETTST 360
           VLLPVTEILAVIIFNE+FQAEK VSLALNLWGF+SYFYGEIK+NK+KKLELQRYEET ST
Sbjct: 301 VLLPVTEILAVIIFNEKFQAEKAVSLALNLWGFVSYFYGEIKNNKKKKLELQRYEETAST 360

Query: 361 QVANV 364
           Q+ NV
Sbjct: 361 QITNV 365

BLAST of CSPI02G21320 vs. ExPASy TrEMBL
Match: A0A6J1BSV4 (Probable purine permease OS=Momordica charantia OX=3673 GN=LOC111004539 PE=3 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 1.1e-156
Identity = 291/363 (80.17%), Postives = 323/363 (88.98%), Query Frame = 0

Query: 1   MDKEQQQQNTPSVKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPI 60
           MDKEQQ+    ++KR LLIFNC LLA+GNCGGPL+MRLYFVHGGKRVWLSS LETGGWPI
Sbjct: 1   MDKEQQRD--AAMKRALLIFNCVLLAIGNCGGPLVMRLYFVHGGKRVWLSSWLETGGWPI 60

Query: 61  IFIPLLISYIHRRRLAALDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVAR 120
           IFIPL ISY HRRR AA        S   IFMKPRLFLA+  IG++TGFDDYLYAYGVAR
Sbjct: 61  IFIPLAISYFHRRRAAA------GESTTLIFMKPRLFLAATVIGVITGFDDYLYAYGVAR 120

Query: 121 LPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGES 180
           LPVSTSALIIACQLAFTA FA+LLVKQKFTSYSINAVVL+TIGGAVLALHTSGDRPAGES
Sbjct: 121 LPVSTSALIIACQLAFTAVFAYLLVKQKFTSYSINAVVLMTIGGAVLALHTSGDRPAGES 180

Query: 181 NKDYIAGFLMTVAAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGM 240
            K Y+AGFLMTVAAA++YGFVLPLVELTYKKA+QQITYTLVLE QL+MSLFAT+VC +GM
Sbjct: 181 TKAYVAGFLMTVAAALLYGFVLPLVELTYKKAKQQITYTLVLEIQLVMSLFATLVCTVGM 240

Query: 241 LINNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVL 300
           LINNDFQVIPREA  FGLG+ +YY+VL  SAI+WQGFFLGAIGVIF SSSLFSGIVIAVL
Sbjct: 241 LINNDFQVIPREAREFGLGETKYYVVLAFSAIIWQGFFLGAIGVIFCSSSLFSGIVIAVL 300

Query: 301 LPVTEILAVIIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKRKKLELQRYEETTSTQV 360
           LPVTEILAV+IF+E+FQAEKGVSLALNLWGF+SYFYGEIKH+K++KLELQ  E  ++ Q+
Sbjct: 301 LPVTEILAVVIFSEKFQAEKGVSLALNLWGFVSYFYGEIKHDKKRKLELQNAE--SACQI 353

Query: 361 ANV 364
            +V
Sbjct: 361 TSV 353

BLAST of CSPI02G21320 vs. ExPASy TrEMBL
Match: A0A0A0LRS6 (Probable purine permease OS=Cucumis sativus OX=3659 GN=Csa_1G071290 PE=3 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 3.4e-155
Identity = 286/364 (78.57%), Postives = 323/364 (88.74%), Query Frame = 0

Query: 4   EQQQQNTPSVKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPIIFI 63
           +QQQQ TP+++RTLL+FNC LLAVG CGGPL+MRLYF+HGG RVWLS+ LETGG PIIFI
Sbjct: 3   QQQQQTTPAMRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETGGCPIIFI 62

Query: 64  PLLISYIHRRRLAA--LDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVARL 123
           PLLISYIHRRR  A  L+PS S NS + IFMK RLFLAS  IGI+TGF D+LYAYGVAR+
Sbjct: 63  PLLISYIHRRRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARI 122

Query: 124 PVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGESN 183
           PVSTSALI ACQLAFTAGFAF LVKQKFT+YSIN+VVL+T GGA+LALHTSGDR AGESN
Sbjct: 123 PVSTSALIRACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESN 182

Query: 184 KDYIAGFLMTVAAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGML 243
           ++YI GFL TVAA+VVYGF+LPLVELTYKKARQQITYTLVLE QLIMSLFAT+VC+I ML
Sbjct: 183 REYIEGFLTTVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIAML 242

Query: 244 INNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVLL 303
           INNDFQ+I  EAE FGLGK +YY++LVLSAI+WQGFFLG IGVIFSSSS FSGI+IAVLL
Sbjct: 243 INNDFQMIAMEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAVLL 302

Query: 304 PVTEILAVIIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKR--KKLELQRYEETTSTQ 363
           PVTEILAV+ FNE+FQAEK +SL LNLWGF+SYFYGEIKHNK+  K LELQR  ETT+TQ
Sbjct: 303 PVTEILAVVTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAETTTTQ 362

BLAST of CSPI02G21320 vs. NCBI nr
Match: XP_004152618.1 (purine permease 3 [Cucumis sativus] >KGN62722.1 hypothetical protein Csa_022030 [Cucumis sativus])

HSP 1 Score: 701.8 bits (1810), Expect = 3.0e-198
Identity = 362/363 (99.72%), Postives = 362/363 (99.72%), Query Frame = 0

Query: 1   MDKEQQQQNTPSVKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPI 60
           MDKEQQQQNTPSVKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPI
Sbjct: 1   MDKEQQQQNTPSVKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPI 60

Query: 61  IFIPLLISYIHRRRLAALDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVAR 120
           IFIPLLISYIHRRRLAALDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVAR
Sbjct: 61  IFIPLLISYIHRRRLAALDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVAR 120

Query: 121 LPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGES 180
           LPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGES
Sbjct: 121 LPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGES 180

Query: 181 NKDYIAGFLMTVAAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGM 240
           NKDYIAGFLMTVAAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGM
Sbjct: 181 NKDYIAGFLMTVAAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGM 240

Query: 241 LINNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVL 300
           LINNDFQVIPREAE FGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVL
Sbjct: 241 LINNDFQVIPREAEAFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVL 300

Query: 301 LPVTEILAVIIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKRKKLELQRYEETTSTQV 360
           LPVTEILAVIIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKRKKLELQRYEETTSTQV
Sbjct: 301 LPVTEILAVIIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKRKKLELQRYEETTSTQV 360

Query: 361 ANV 364
           ANV
Sbjct: 361 ANV 363

BLAST of CSPI02G21320 vs. NCBI nr
Match: XP_008444875.1 (PREDICTED: purine permease 3-like [Cucumis melo] >KAA0065122.1 purine permease 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 654.1 bits (1686), Expect = 7.3e-184
Identity = 340/365 (93.15%), Postives = 351/365 (96.16%), Query Frame = 0

Query: 1   MDKEQQ--QQNTPSVKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGW 60
           MDKEQQ  QQNT +VKRTLLIFNC LLAVGNCGGPL+MRLYFVHGG RVWLSS LETGGW
Sbjct: 1   MDKEQQQHQQNTSAVKRTLLIFNCFLLAVGNCGGPLVMRLYFVHGGNRVWLSSFLETGGW 60

Query: 61  PIIFIPLLISYIHRRRLAALDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGV 120
           PIIFIPLLISYI RRRLAALDPSGS +S+Q IFMKPRLFLASA IGILTGFDDYLYAYGV
Sbjct: 61  PIIFIPLLISYIRRRRLAALDPSGSADSSQIIFMKPRLFLASAVIGILTGFDDYLYAYGV 120

Query: 121 ARLPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAG 180
           ARLPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAG
Sbjct: 121 ARLPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAG 180

Query: 181 ESNKDYIAGFLMTVAAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAI 240
           ESNKDYIAGFLMTVAAAVVYGF+LPLVELTYKKARQQITYTLVLE+QLIMSLFATIVC I
Sbjct: 181 ESNKDYIAGFLMTVAAAVVYGFILPLVELTYKKARQQITYTLVLEYQLIMSLFATIVCVI 240

Query: 241 GMLINNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIA 300
           GMLINNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIA
Sbjct: 241 GMLINNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIA 300

Query: 301 VLLPVTEILAVIIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKRKKLELQRYEETTST 360
           VLLPVTEILAVIIFNE+FQAEK VSLALNLWGF+SYFYGEIK+NK+KKLELQRYEET ST
Sbjct: 301 VLLPVTEILAVIIFNEKFQAEKAVSLALNLWGFVSYFYGEIKNNKKKKLELQRYEETAST 360

Query: 361 QVANV 364
           Q+ NV
Sbjct: 361 QITNV 365

BLAST of CSPI02G21320 vs. NCBI nr
Match: XP_038885544.1 (purine permease 3-like [Benincasa hispida])

HSP 1 Score: 637.1 bits (1642), Expect = 9.2e-179
Identity = 327/364 (89.84%), Postives = 350/364 (96.15%), Query Frame = 0

Query: 1   MDKEQQ-QQNTPSVKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWP 60
           MDKEQQ QQNT ++KRTLL+FNC LLAVGNCGGPL+MRLYFVHGGKRVWLSS LETGGWP
Sbjct: 1   MDKEQQPQQNTTAMKRTLLVFNCFLLAVGNCGGPLVMRLYFVHGGKRVWLSSFLETGGWP 60

Query: 61  IIFIPLLISYIHRRRLAALDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVA 120
           IIFIPL+ISYI+RRR+AAL+PS S +SA+ IFMKPRLFLA+A IGILTGFDDYLYAYGVA
Sbjct: 61  IIFIPLIISYINRRRVAALNPSVSTDSAKMIFMKPRLFLATAVIGILTGFDDYLYAYGVA 120

Query: 121 RLPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGE 180
           RLPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLT+GGAVLALHTSGDRPAGE
Sbjct: 121 RLPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTMGGAVLALHTSGDRPAGE 180

Query: 181 SNKDYIAGFLMTVAAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIG 240
           SN+DYIAGFLMTVAAA++YGFVLPLVELTY+KARQQITYTLVLEFQLIMSLFATIVC IG
Sbjct: 181 SNRDYIAGFLMTVAAAIMYGFVLPLVELTYRKARQQITYTLVLEFQLIMSLFATIVCTIG 240

Query: 241 MLINNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAV 300
           MLINNDFQVIPREAE FGLGKFRYYLVLVLSAI+WQ FFLGAIGVIFSSSSLFSGIVIAV
Sbjct: 241 MLINNDFQVIPREAEEFGLGKFRYYLVLVLSAIIWQAFFLGAIGVIFSSSSLFSGIVIAV 300

Query: 301 LLPVTEILAVIIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKRKKLELQRYEETTSTQ 360
           LLPVTEILAVIIFNE+FQAEK VSLALNLWGF+SYFYGEIKHNK+KKLELQ+YEET  TQ
Sbjct: 301 LLPVTEILAVIIFNEKFQAEKAVSLALNLWGFVSYFYGEIKHNKKKKLELQKYEETRPTQ 360

Query: 361 VANV 364
           ++NV
Sbjct: 361 ISNV 364

BLAST of CSPI02G21320 vs. NCBI nr
Match: XP_022131268.1 (purine permease 3-like [Momordica charantia])

HSP 1 Score: 562.8 bits (1449), Expect = 2.2e-156
Identity = 291/363 (80.17%), Postives = 323/363 (88.98%), Query Frame = 0

Query: 1   MDKEQQQQNTPSVKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPI 60
           MDKEQQ+    ++KR LLIFNC LLA+GNCGGPL+MRLYFVHGGKRVWLSS LETGGWPI
Sbjct: 1   MDKEQQRD--AAMKRALLIFNCVLLAIGNCGGPLVMRLYFVHGGKRVWLSSWLETGGWPI 60

Query: 61  IFIPLLISYIHRRRLAALDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVAR 120
           IFIPL ISY HRRR AA        S   IFMKPRLFLA+  IG++TGFDDYLYAYGVAR
Sbjct: 61  IFIPLAISYFHRRRAAA------GESTTLIFMKPRLFLAATVIGVITGFDDYLYAYGVAR 120

Query: 121 LPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGES 180
           LPVSTSALIIACQLAFTA FA+LLVKQKFTSYSINAVVL+TIGGAVLALHTSGDRPAGES
Sbjct: 121 LPVSTSALIIACQLAFTAVFAYLLVKQKFTSYSINAVVLMTIGGAVLALHTSGDRPAGES 180

Query: 181 NKDYIAGFLMTVAAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGM 240
            K Y+AGFLMTVAAA++YGFVLPLVELTYKKA+QQITYTLVLE QL+MSLFAT+VC +GM
Sbjct: 181 TKAYVAGFLMTVAAALLYGFVLPLVELTYKKAKQQITYTLVLEIQLVMSLFATLVCTVGM 240

Query: 241 LINNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVL 300
           LINNDFQVIPREA  FGLG+ +YY+VL  SAI+WQGFFLGAIGVIF SSSLFSGIVIAVL
Sbjct: 241 LINNDFQVIPREAREFGLGETKYYVVLAFSAIIWQGFFLGAIGVIFCSSSLFSGIVIAVL 300

Query: 301 LPVTEILAVIIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKRKKLELQRYEETTSTQV 360
           LPVTEILAV+IF+E+FQAEKGVSLALNLWGF+SYFYGEIKH+K++KLELQ  E  ++ Q+
Sbjct: 301 LPVTEILAVVIFSEKFQAEKGVSLALNLWGFVSYFYGEIKHDKKRKLELQNAE--SACQI 353

Query: 361 ANV 364
            +V
Sbjct: 361 TSV 353

BLAST of CSPI02G21320 vs. NCBI nr
Match: XP_004146629.2 (purine permease 3 [Cucumis sativus])

HSP 1 Score: 557.8 bits (1436), Expect = 7.1e-155
Identity = 286/364 (78.57%), Postives = 323/364 (88.74%), Query Frame = 0

Query: 4   EQQQQNTPSVKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPIIFI 63
           +QQQQ TP+++RTLL+FNC LLAVG CGGPL+MRLYF+HGG RVWLS+ LETGG PIIFI
Sbjct: 3   QQQQQTTPAMRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETGGCPIIFI 62

Query: 64  PLLISYIHRRRLAA--LDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVARL 123
           PLLISYIHRRR  A  L+PS S NS + IFMK RLFLAS  IGI+TGF D+LYAYGVAR+
Sbjct: 63  PLLISYIHRRRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARI 122

Query: 124 PVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGESN 183
           PVSTSALI ACQLAFTAGFAF LVKQKFT+YSIN+VVL+T GGA+LALHTSGDR AGESN
Sbjct: 123 PVSTSALIRACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESN 182

Query: 184 KDYIAGFLMTVAAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGML 243
           ++YI GFL TVAA+VVYGF+LPLVELTYKKARQQITYTLVLE QLIMSLFAT+VC+I ML
Sbjct: 183 REYIEGFLTTVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIAML 242

Query: 244 INNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVLL 303
           INNDFQ+I  EAE FGLGK +YY++LVLSAI+WQGFFLG IGVIFSSSS FSGI+IAVLL
Sbjct: 243 INNDFQMIAMEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAVLL 302

Query: 304 PVTEILAVIIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKR--KKLELQRYEETTSTQ 363
           PVTEILAV+ FNE+FQAEK +SL LNLWGF+SYFYGEIKHNK+  K LELQR  ETT+TQ
Sbjct: 303 PVTEILAVVTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAETTTTQ 362

BLAST of CSPI02G21320 vs. TAIR 10
Match: AT1G28220.1 (purine permease 3 )

HSP 1 Score: 423.3 bits (1087), Expect = 1.9e-118
Identity = 212/348 (60.92%), Postives = 279/348 (80.17%), Query Frame = 0

Query: 15  RTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPIIFIPLLISYIHRRR 74
           + L+I NC +LA+GNCGGPLIMRLYF +GGKR+W S+ LET G+P+IFIPLL SYI RRR
Sbjct: 3   KALVIINCIILAIGNCGGPLIMRLYFNNGGKRIWFSTFLETAGFPVIFIPLLFSYITRRR 62

Query: 75  LAALDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVARLPVSTSALIIACQL 134
                 +   +S  F  +KPRL +A+  +GIL+GFD+YLYAYG+A LPVST+ALIIA QL
Sbjct: 63  -----SNNVGDSTSFFLIKPRLLIAAVIVGILSGFDNYLYAYGIAYLPVSTAALIIASQL 122

Query: 135 AFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGESNKDYIAGFLMTVAA 194
           AF A F+F +VK KFT ++INAVVLLT+G AVL +HT  D+P  E++K YI GFL+TVAA
Sbjct: 123 AFIAIFSFFMVKHKFTPFTINAVVLLTVGAAVLGMHTETDKPVHETHKQYITGFLITVAA 182

Query: 195 AVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGMLINNDFQVIPREAE 254
           AV+Y F+LPLVEL Y+KA+Q ++YTLVLEFQLI+ L A+IV  IGM I  DF+ +P+EA 
Sbjct: 183 AVMYAFILPLVELAYQKAKQTMSYTLVLEFQLILCLLASIVSVIGMFIAGDFKALPKEAR 242

Query: 255 TFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVLLPVTEILAVIIFNE 314
            F LG+  +Y+V V SAI+WQGFFLGAIG+IFS+SSL SGI+I+VLLP+TE+LAVI ++E
Sbjct: 243 EFKLGEALFYVVAVFSAIIWQGFFLGAIGLIFSTSSLVSGIMISVLLPITEVLAVIFYHE 302

Query: 315 RFQAEKGVSLALNLWGFLSYFYGEIKHNK-RKKLELQRYEETTSTQVA 362
           +FQAEKG+SLAL+LWGF+SYFYGEIK  + +++++ +  +ET  + ++
Sbjct: 303 KFQAEKGLSLALSLWGFVSYFYGEIKSGEDKRRIQQEESQETEQSSLS 345

BLAST of CSPI02G21320 vs. TAIR 10
Match: AT1G28230.1 (purine permease 1 )

HSP 1 Score: 402.9 bits (1034), Expect = 2.7e-112
Identity = 204/335 (60.90%), Postives = 260/335 (77.61%), Query Frame = 0

Query: 13  VKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPIIFIPLLISYIHR 72
           +K  L+I NC +L +G CGGPL+ RLYF +GGKR+W  S L T G+PII IPLL+S++ R
Sbjct: 1   MKNGLIIINCIILTIGTCGGPLLTRLYFTNGGKRIWFMSFLSTAGFPIILIPLLVSFLSR 60

Query: 73  RRLAALDPSGSPN--SAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVARLPVSTSALII 132
           RR +  +P+ + N    +   M+  LF+AS  IG+LTG D+YLY+YG+A LPVSTS+LII
Sbjct: 61  RR-SNRNPNNAENKRKTKLFLMETPLFIASIVIGLLTGLDNYLYSYGLAYLPVSTSSLII 120

Query: 133 ACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGESNKDYIAGFLM 192
             QLAF A FAFLLVKQKFT +SINAVVLLT+G  +LALH+ GD+PA ES K+Y+ GFLM
Sbjct: 121 GTQLAFNALFAFLLVKQKFTPFSINAVVLLTVGIGILALHSDGDKPAKESKKEYVVGFLM 180

Query: 193 TVAAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGMLINNDFQVIP 252
           TV AA++Y F+LPLVELTYKKARQ+IT+ LVLE Q++M L AT  C IGM I  DF+VI 
Sbjct: 181 TVVAALLYAFILPLVELTYKKARQEITFPLVLEIQMVMCLAATFFCVIGMFIVGDFKVIA 240

Query: 253 REAETFGL-GKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVLLPVTEILAV 312
           REA  F + G   YY ++V++ I+WQGFFLGAIG++F +SSL SG++I+VLLPVTE+ AV
Sbjct: 241 REAREFKIGGSVFYYALIVITGIIWQGFFLGAIGIVFCASSLASGVLISVLLPVTEVFAV 300

Query: 313 IIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKR 345
           + F E+FQAEKGVSL L+LWGF+SYFYGE K  K+
Sbjct: 301 VCFREKFQAEKGVSLLLSLWGFVSYFYGEFKSGKK 334

BLAST of CSPI02G21320 vs. TAIR 10
Match: AT2G33750.2 (purine permease 2 )

HSP 1 Score: 385.2 bits (988), Expect = 5.9e-107
Identity = 190/336 (56.55%), Postives = 258/336 (76.79%), Query Frame = 0

Query: 13  VKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPIIFIPLLISYIHR 72
           +K  L+I NC  LA+GNCGGPL+MRLYF +GG+R+W  S L+T G P+IF PLL+S++ R
Sbjct: 3   MKTVLVIINCIFLAIGNCGGPLMMRLYFQNGGERIWFPSFLQTVGCPLIFFPLLLSFLRR 62

Query: 73  RRLAALDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVARLPVSTSALIIAC 132
           RR           +  F  MKP LF+A+  +G+L GFD+YLY+YG+A +PVST++LII+ 
Sbjct: 63  RRCLE-----EQETTPFFLMKPPLFIAAIVVGLLVGFDNYLYSYGLAYIPVSTASLIISA 122

Query: 133 QLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGESNKDYIAGFLMTV 192
           QL FTA FAF +VKQKFT ++INA+VLLT G  VLAL++  D+ A E++K+Y+ GF+MT+
Sbjct: 123 QLGFTALFAFFMVKQKFTPFTINAIVLLTGGAVVLALNSDSDKLANETHKEYVVGFIMTL 182

Query: 193 AAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGMLINNDFQVIPRE 252
            AA++YGF+LPLVEL+YKK+ Q+ITYTL LEFQ+++   AT VC +GML   DF+VI  E
Sbjct: 183 GAALLYGFILPLVELSYKKSGQRITYTLALEFQMVLCFAATCVCLVGMLAAGDFKVIAGE 242

Query: 253 AETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVLLPVTEILAVIIF 312
           A  F LG+  YY+V+V +AI+WQ FF+GAIG+IF +SSL SGI+++ LLPVT ILAVI F
Sbjct: 243 ARDFKLGESLYYVVIVFTAIIWQAFFVGAIGLIFCASSLVSGIMVSALLPVTVILAVICF 302

Query: 313 NERFQAEKGVSLALNLWGFLSYFYGEIKHNKRKKLE 349
            E+FQA KGV+LAL+LWG +SYFYG++K  ++ K +
Sbjct: 303 QEKFQAGKGVALALSLWGSVSYFYGQVKSEEKTKAQ 333

BLAST of CSPI02G21320 vs. TAIR 10
Match: AT2G33750.1 (purine permease 2 )

HSP 1 Score: 376.7 bits (966), Expect = 2.1e-104
Identity = 190/347 (54.76%), Postives = 258/347 (74.35%), Query Frame = 0

Query: 13  VKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPIIFIPLLISYIHR 72
           +K  L+I NC  LA+GNCGGPL+MRLYF +GG+R+W  S L+T G P+IF PLL+S++ R
Sbjct: 3   MKTVLVIINCIFLAIGNCGGPLMMRLYFQNGGERIWFPSFLQTVGCPLIFFPLLLSFLRR 62

Query: 73  RRLAALDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVARLPVSTSALIIAC 132
           RR           +  F  MKP LF+A+  +G+L GFD+YLY+YG+A +PVST++LII+ 
Sbjct: 63  RRCLE-----EQETTPFFLMKPPLFIAAIVVGLLVGFDNYLYSYGLAYIPVSTASLIISA 122

Query: 133 QLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGESNKDYIAGFLMTV 192
           QL FTA FAF +VKQKFT ++INA+VLLT G  VLAL++  D+ A E++K+Y+ GF+MT+
Sbjct: 123 QLGFTALFAFFMVKQKFTPFTINAIVLLTGGAVVLALNSDSDKLANETHKEYVVGFIMTL 182

Query: 193 AAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGMLINNDF------ 252
            AA++YGF+LPLVEL+YKK+ Q+ITYTL LEFQ+++   AT VC +GML   DF      
Sbjct: 183 GAALLYGFILPLVELSYKKSGQRITYTLALEFQMVLCFAATCVCLVGMLAAGDFKVKHAL 242

Query: 253 -----QVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVLL 312
                +VI  EA  F LG+  YY+V+V +AI+WQ FF+GAIG+IF +SSL SGI+++ LL
Sbjct: 243 FIFKNRVIAGEARDFKLGESLYYVVIVFTAIIWQAFFVGAIGLIFCASSLVSGIMVSALL 302

Query: 313 PVTEILAVIIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKRKKLE 349
           PVT ILAVI F E+FQA KGV+LAL+LWG +SYFYG++K  ++ K +
Sbjct: 303 PVTVILAVICFQEKFQAGKGVALALSLWGSVSYFYGQVKSEEKTKAQ 344

BLAST of CSPI02G21320 vs. TAIR 10
Match: AT4G18205.1 (Nucleotide-sugar transporter family protein )

HSP 1 Score: 195.7 bits (496), Expect = 6.6e-50
Identity = 117/325 (36.00%), Postives = 185/325 (56.92%), Query Frame = 0

Query: 37  RLYFVHGGKRVWLSSCLETGGWPIIFIPLLISYIHRRRLAALDPSGSPNSAQFIFMKPRL 96
           RLY+ +GGK  ++ + L+  G+P++ +    S I + +    + S SP+           
Sbjct: 61  RLYYENGGKSTYVVTLLQLIGFPVLILFRFFSRIRQPKSTDTNFSQSPSFTT-------- 120

Query: 97  FLASAFI--GILTGFDDYLYAYGVARLPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSI 156
            LAS ++  G+L     YL A G+  LPVST +LI+A QLAFTA F++ L  QKFT   +
Sbjct: 121 -LASVYLCTGLLVSAYAYLSAVGLLYLPVSTFSLILASQLAFTAFFSYFLNSQKFTPLIV 180

Query: 157 NAVVLLTIGGAVLALHTSGDRPAGESNKDYIAGFLMTVAAAVVYGFVLPLVELTYKKARQ 216
           N++ LLT+  A+L ++T  +     S   Y+ GF+ T+ A+   G VL L++L ++K   
Sbjct: 181 NSLFLLTVSSALLVVNTDSENTTNVSRVQYVIGFICTIGASAGIGLVLSLIQLLFRKVFT 240

Query: 217 QITYTLVLEFQLIMSLFATIVCAIGMLINNDFQVIPREAETFGLGKFRYYLVLVLSAILW 276
           + T + VL+     SL AT V  IG+  + +++ +P E   + LGK  Y L L  +AI W
Sbjct: 241 KHTSSAVLDLANYQSLVATCVVLIGLFASGEWRTLPSEMRNYKLGKVSYILTLASAAIFW 300

Query: 277 QGFFLGAIGVIFSSSSLFSGIVIAVLLPVTEILAVIIFNERFQAEKGVSLALNLWGFLSY 336
           Q + +G +G+IF SSS+FS  + AV LP+  ++AVI+F+++  A K  S+ L +WGFLS+
Sbjct: 301 QVYTVGCVGLIFESSSVFSNSITAVGLPIVPVVAVIVFHDKMDASKIFSIILAIWGFLSF 360

Query: 337 FYGE-IKHNKRKKLELQRYEETTST 359
            Y   +   K K  + +  EE T T
Sbjct: 361 VYQHYLDEKKLKTCQTKPVEEETQT 376

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FZ952.7e-11760.92Purine permease 3 OS=Arabidopsis thaliana OX=3702 GN=PUP3 PE=2 SV=1[more]
Q9FZ963.8e-11160.90Purine permease 1 OS=Arabidopsis thaliana OX=3702 GN=PUP1 PE=1 SV=1[more]
Q94GB12.9e-10354.76Purine permease 2 OS=Arabidopsis thaliana OX=3702 GN=PUP2 PE=1 SV=1[more]
Q8RY749.3e-4936.00Probable purine permease 22 OS=Arabidopsis thaliana OX=3702 GN=PUP22 PE=2 SV=1[more]
Q9LPF61.2e-4833.75Probable purine permease 11 OS=Arabidopsis thaliana OX=3702 GN=PUP11 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LPJ91.5e-19899.72Probable purine permease OS=Cucumis sativus OX=3659 GN=Csa_2G369730 PE=3 SV=1[more]
A0A5A7V9X13.5e-18493.15Probable purine permease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A1S3BC923.5e-18493.15Probable purine permease OS=Cucumis melo OX=3656 GN=LOC103488091 PE=3 SV=1[more]
A0A6J1BSV41.1e-15680.17Probable purine permease OS=Momordica charantia OX=3673 GN=LOC111004539 PE=3 SV=... [more]
A0A0A0LRS63.4e-15578.57Probable purine permease OS=Cucumis sativus OX=3659 GN=Csa_1G071290 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004152618.13.0e-19899.72purine permease 3 [Cucumis sativus] >KGN62722.1 hypothetical protein Csa_022030 ... [more]
XP_008444875.17.3e-18493.15PREDICTED: purine permease 3-like [Cucumis melo] >KAA0065122.1 purine permease 3... [more]
XP_038885544.19.2e-17989.84purine permease 3-like [Benincasa hispida][more]
XP_022131268.12.2e-15680.17purine permease 3-like [Momordica charantia][more]
XP_004146629.27.1e-15578.57purine permease 3 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT1G28220.11.9e-11860.92purine permease 3 [more]
AT1G28230.12.7e-11260.90purine permease 1 [more]
AT2G33750.25.9e-10756.55purine permease 2 [more]
AT2G33750.12.1e-10454.76purine permease 2 [more]
AT4G18205.16.6e-5036.00Nucleotide-sugar transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF16913PUNUTcoord: 17..343
e-value: 2.1E-119
score: 398.5
NoneNo IPR availablePANTHERPTHR31376:SF1PURINE PERMEASE 2coord: 3..353
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 92..171
IPR030182Purine permease, plantPANTHERPTHR31376OS09G0467300 PROTEIN-RELATEDcoord: 3..353

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G21320.1CSPI02G21320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1904823 purine nucleobase transmembrane transport
biological_process GO:0015860 purine nucleoside transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005345 purine nucleobase transmembrane transporter activity
molecular_function GO:0015211 purine nucleoside transmembrane transporter activity