CSPI02G21050 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G21050
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	MAGE domain-containing protein
Location	Chr2: 18527170 .. 18531853 (+)
RNA-Seq Expression	CSPI02G21050
Synteny	CSPI02G21050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTATATAGTGAATACGGTACGAGTGCTATTTAATATGTAAAGAGAAGAGAAGAAAGAACGAAAAAGAAAAAGCGCGCTCGCCGCCCGGAGTGCAAAACCCGGTCGGACTTCGAACTGCGCGGGAACATACCGATGACTGCAACGGAGAGACTTTAGAGGGGGGTTTCTGAAGATTCTCTAAAAAAATTTGCTCATTTTCCCCGGAAAATGCACCGAAGAAGATTATTCGTTTGAAACGTTCGTGTTCTCTCCCGTCACTGCTTGGACCGCTCGCATTCGAATTACTTCGGGTAAGCTATACGGATTCAGTCTCTTTGTCTATCAATTCGACCTAGTAGAATCATTTTTTGTCATTGGTTGAGTACGATTTTGTTGTTCACGTGTATACACATTTGTTGATTTTGGAAATCCACCGTTGTTTCTGTCATGACTTCAGTGGTTGACATTGTCTGTATATTTTTCTTTGATCTCGAGCTGATCGTTAATTTGTTATAGGAAGGAAAACTGTTTGTTATAGATGGCAAGTGCAGGAGAATATGAACTCGACATTTCAGAGGAGGTATGAGGTTATCCTCTCCGCTTTTTGCTGTGGAGTTGGTGTAATCCAGGGCTTGCTCTTCGTTTGGCAAAACAGACTGTTGATATTTCTGATTGCTAAAGCATTTCTAATAACAGGAAAAGGACAAGCTCGTAGCGGAAGCTATTCGATATATCATATTCAAAACTCATCAAAATTCTGGATGCCCAATCAAAAGGGAAGAACTCACTCAGCTCGTAACGAAAAATTATCGGAATCGTGGATTGCCAGCTATTGTGATTGACGAGGCTAAAAGGAAGCTGTCAAGCATTTTTGGTTACGAGTTGAGGGAACTTCAAAGAACGCGGCCTTCATCCACTGGCCAACCGCATTCTTCCCAACAAAGTAAGAAATGGTCGAACTTATAATATTGCTTCTCTGATAATTGCTGTTGAGATTTTCAAGCGTTGGTATGATTTCACTACTCTCATTATCAAATTTCTTTTTGACACTGTGGCTTTTCAAGTCACAACAGATTTCTTTTCACAAACAGGCCATTGGTTCAGTCTTGTAATGAGTCAAAGCTAAAGCCATACGGTTTTTTCAAAATGAGATATCTTTTTGCAACTTTAGCACAGAAGAAGTGTCTAACTAGTAACTAGTACGTTCAAGTAATGATCTTTTGAAATTATCCTTACGAGAACATGTTCACGTGGGTGAGAAGTGCCGCTTTCTTCTAAGTAATGATCTTGTTATCACTCCGGTCCGTGGGATATGCTTATGCTTCTCTACATTAAGTGTAGTTCAATGATCAGTTTATTACTCATGCTACACACCTTATCTTAAAAACCAAATATCTAAAAATTTAAGTATCCAAATTTAATCTCCTCCCAGATGGCCTAGGTTGCTGAAACAGTTTGTAAGCTCTTCAAAATGAACATCCGAGCTTCTTGACATACAATCCTCATTATTCAGTACATCGTAATACAATAACCGGAACATTGGGGTTGGAGTTTTTGAGCATGAAAGTCATTGTTATGGATTGTTAAAAGTCATAGTGAATAACAGTAGTTCTGTAGAATTTTGAATATGGTTATTAGATTACAAGTTTCCTTAGTTGATGAAGAAGCTCTTATTTCATCAAAGGAATAACTGTTATTTAAATCTTAAAAACGGGTGTTTTGGTTCCTCAAAACCCATGCCATTATTTACCTATAATTAAGGCTGCCAATCTTTCATCAACCTGCTGCCCATTTCCTGTCTTTTTAACATCTGAAATAGCCATCACAAGCTTTCAGCTGCTGGAATTACCCCGTACCCCCTCTAGTGGCTGTGTTTTATTACTTCGGTGCCTCTAGAAGGCTCACAACAGTATAGAAATCCTCTCCTCTAGGATTTCTGTAGCTTTTGTATACATGGACCTCGGAGGTGTTGTAGGCTTCATGGCTAGCCCGTGGTATCCTACTCTGTAGATTATTTCCATCCCTTATGGCTTGTATTGTAGTGTAGCTTTTGTCTTCATAGATCTTCACAGATCTTCATCTATTACTTTTTTATGCTCTATGGCTATTCATTAATATTCTAGCTTTCAGATTACTTCCATCTGGCATGGCTTGCCACAGCACTATTTCACTATTGGATCTCACTTTTACCTTGTCACTACACATTTTCTCAGACGTTGGAGATGCAAAATCCTATGTTCTCATAAGCAAGCTTCCTGCTGATATCTACAGAAAGTATGTTGAGGATGTAAATACTGCACACGTGACTGGTTTTACTTTTGTTATTATTAGTATTGTACATCTTGCTGGCGGCAAAATTCCTGAAGGTATGGCGTATGCTATCTTAACCCAATGCATGTAGCTTTGAGAGTGTAGGACAACTTGTGCTTGAAATGGATTTCATTCATGAACTTATTATTTCATGTAGTTCTGGCGATACCAATTTAGTAAAATGATGGGAAAATGTTACTTATCAGATAATGATAGTGCATTTATGTACTATCGATCAATTCAAAATATTTCACAGAATTTCTTAGGGCAAGAAATCCGATTTTTTGCTTCTATTTCACCCTTTGTTTTATAATGGCACCTGGCAATACCCTGCAGAAAGCCTTCGGCATCACTTGAAAAGAATGGGATTGTCTGAAAGTGATGAAAACCATCCAGTCCTTGGAAACATCAAGCATGCAATAGAGTTGGTTGTCCAGCAAAGGTCAGTAGACCCTGTACTATATTATGGAACTGCACTTGAAAATTTGTTAAGTAATTTGATGATCATTGAAGGTACAGGAAAAACTGGTTACTGGTTCCCTTTAACTTTTTCCTTGCTTTGTAGATATCTGCAGAAGGTCAAAGTGAACGCTCCTGAAGGCAATATTACCTTCTACGAGCTTGCTGAGAGAGCTACATATGGACCAGTTAGTGAAAAGATTAAAGAATATGTAGCACAGGTATGCAACTTGTAAATTGAAAATAAAGGTTTTTGAGTAGCAACGGTATAGTCATAGAAAAAATCCTCATTTTAATATATTTTTTGAAAATTTTTATCTGGGAAAAAGAAAAGGAAAAGAGAAGAAACTTAAACAATCATGAAATACAAAGTTCCCCTCGTTCCATTGCGCGGCCATTTTGGATTCTTGCGAAAATGTTGACAAGTTTTCTGCTGGTTTTGCTCAACAACGGGATTATGAGAATAGTAAACTTTGGAATATAAGAATAGGATGGTTGGAAGCAGGAATGGTCGTTGGTATATGAAATGATATTTGCAGAATTTGATTTAATTTTTTAATCATCTATTTCTAATTTAGATATTGAATTTTATTATTTTTATTAATATTATTTTGTATAATAAATTTAGAACTGAATAATCAAAACTTTTTTAACAGCAAGCATAGAAGTTTCACAAATTTTCTCGTTTAAATTTATTGTTCGTTTTTACTTTGAGGAAGACTTAAAAGAACATAAGGAATAAAATCTCTATAATCTTACTGCGGAGCATTTTAGATTGTCGGCCACTATATAGGATTTCAGTGTATGAAATTTTAGTTGTGCTTAAATCTATTTAATCATAGGTCAAGAATTGTACATAAGGTGGATGGTTGGATGGACGAATCAGCGCTATTACGAATGATAGAAAAGTTTTGCGCAAGTAGCACAAAGGTGTACAGTGTAGATGGTAGGATAGAAAAGTCAATGCTGCTATGAAATTATGATTTAATAATGATGCCCCCTCCCTTTTCCCCTCCCAAAGGATTTGGGGTTAATCTGTCTAATTTTTCTGATTTATGCTTAATTCAGTTTGCTCTTGGCACATCAGATCATAGCATTTGAAGCATGATTTGTCCTATTATAATTTTGTGTCAAGTAGCATGCTTCATGTTCTTGCCTTGTTGCTGTACGATTCTCAAAGCAAACAAATTTTTAAATCCTTGAGATGAGTTGCACCATTAACCATGGTTCTTCTTGTTTTTCTTTTACTAAATTTTATGAACACTACGCGGTACCATACCATCACCTATGATATTCATTTTATACTTGTATTTTCAAAAGTAAGTCAGGCATATAGGTTTTCCATTAACTTCCTGCCAATTATTTAGTTTAATTGATCTTCATGTTAGGATTTGAACTGCACTATAAAATTAGAAGATTTATAAGTTTATTCATGGATTATCTTTACCAGCATAGCTATGCTTTTCCTGACTAGAGATGTTCATCCTCTGTAGATTGTCAATTCAAATGTTACTGCCGCAGACACCTAAGCTAACCAGTGTCGCAACGATCTGAAATGGCCTCAAACTTTGCAGAGCCTCGTCGTTCACTGAATGTATTTAGTTAAAATATGTACCTAATACTTTAATGATTCCAATTAAATACGTTGTATTCATTGCATTAAATACTGTTAGTTACTTGCCTTTAATATTGTAGGTGAGTTACGGTAGTAGGGGGAAATAGCACATCACGGTAGGTTTCAGCTGAACGGTTTGGTTCTACATATTTAGGTGTGATTGTACATGGTTGGCCCGATTTCTCCATTCTCTTGACTTGACTTGACTTGCAATTTCTCCCATTTTATTGCAATCACAAAATCATTGACTTGTGGTAAGTTGCTGCGAAATTTGCAGTTGACACGAATAGCAAATTCATGAATGGTAGAGAACCATACAG

mRNA sequence

ATTATATAGTGAATACGGTACGAGTGCTATTTAATATGTAAAGAGAAGAGAAGAAAGAACGAAAAAGAAAAAGCGCGCTCGCCGCCCGGAGTGCAAAACCCGGTCGGACTTCGAACTGCGCGGGAACATACCGATGACTGCAACGGAGAGACTTTAGAGGGGGGTTTCTGAAGATTCTCTAAAAAAATTTGCTCATTTTCCCCGGAAAATGCACCGAAGAAGATTATTCGTTTGAAACGTTCGTGTTCTCTCCCGTCACTGCTTGGACCGCTCGCATTCGAATTACTTCGGGAAGGAAAACTGTTTGTTATAGATGGCAAGTGCAGGAGAATATGAACTCGACATTTCAGAGGAGGAAAAGGACAAGCTCGTAGCGGAAGCTATTCGATATATCATATTCAAAACTCATCAAAATTCTGGATGCCCAATCAAAAGGGAAGAACTCACTCAGCTCGTAACGAAAAATTATCGGAATCGTGGATTGCCAGCTATTGTGATTGACGAGGCTAAAAGGAAGCTGTCAAGCATTTTTGGTTACGAGTTGAGGGAACTTCAAAGAACGCGGCCTTCATCCACTGGCCAACCGCATTCTTCCCAACAAAACGTTGGAGATGCAAAATCCTATGTTCTCATAAGCAAGCTTCCTGCTGATATCTACAGAAAGTATGTTGAGGATGTAAATACTGCACACGTGACTGGTTTTACTTTTGTTATTATTAGTATTGTACATCTTGCTGGCGGCAAAATTCCTGAAGAAAGCCTTCGGCATCACTTGAAAAGAATGGGATTGTCTGAAAGTGATGAAAACCATCCAGTCCTTGGAAACATCAAGCATGCAATAGAGTTGGTTGTCCAGCAAAGATATCTGCAGAAGGTCAAAGTGAACGCTCCTGAAGGCAATATTACCTTCTACGAGCTTGCTGAGAGAGCTACATATGGACCAGTTAGTGAAAAGATTAAAGAATATGTAGCACAGATTGTCAATTCAAATGTTACTGCCGCAGACACCTAAGCTAACCAGTGTCGCAACGATCTGAAATGGCCTCAAACTTTGCAGAGCCTCGTCGTTCACTGAATGTATTTAGTTAAAATATGTACCTAATACTTTAATGATTCCAATTAAATACGTTGTATTCATTGCATTAAATACTGTTAGTTACTTGCCTTTAATATTGTAGGTGAGTTACGGTAGTAGGGGGAAATAGCACATCACGGTAGGTTTCAGCTGAACGGTTTGGTTCTACATATTTAGGTGTGATTGTACATGGTTGGCCCGATTTCTCCATTCTCTTGACTTGACTTGACTTGCAATTTCTCCCATTTTATTGCAATCACAAAATCATTGACTTGTGGTAAGTTGCTGCGAAATTTGCAGTTGACACGAATAGCAAATTCATGAATGGTAGAGAACCATACAG

Coding sequence (CDS)

ATGGCAAGTGCAGGAGAATATGAACTCGACATTTCAGAGGAGGAAAAGGACAAGCTCGTAGCGGAAGCTATTCGATATATCATATTCAAAACTCATCAAAATTCTGGATGCCCAATCAAAAGGGAAGAACTCACTCAGCTCGTAACGAAAAATTATCGGAATCGTGGATTGCCAGCTATTGTGATTGACGAGGCTAAAAGGAAGCTGTCAAGCATTTTTGGTTACGAGTTGAGGGAACTTCAAAGAACGCGGCCTTCATCCACTGGCCAACCGCATTCTTCCCAACAAAACGTTGGAGATGCAAAATCCTATGTTCTCATAAGCAAGCTTCCTGCTGATATCTACAGAAAGTATGTTGAGGATGTAAATACTGCACACGTGACTGGTTTTACTTTTGTTATTATTAGTATTGTACATCTTGCTGGCGGCAAAATTCCTGAAGAAAGCCTTCGGCATCACTTGAAAAGAATGGGATTGTCTGAAAGTGATGAAAACCATCCAGTCCTTGGAAACATCAAGCATGCAATAGAGTTGGTTGTCCAGCAAAGATATCTGCAGAAGGTCAAAGTGAACGCTCCTGAAGGCAATATTACCTTCTACGAGCTTGCTGAGAGAGCTACATATGGACCAGTTAGTGAAAAGATTAAAGAATATGTAGCACAGATTGTCAATTCAAATGTTACTGCCGCAGACACCTAA

Protein sequence

MASAGEYELDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLPAIVIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKYVEDVNTAHVTGFTFVIISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIELVVQQRYLQKVKVNAPEGNITFYELAERATYGPVSEKIKEYVAQIVNSNVTAADT*

Homology

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BLAST of CSPI02G21050 vs. ExPASy Swiss-Prot
Match: P43363 (Melanoma-associated antigen 10 OS=Homo sapiens OX=9606 GN=MAGEA10 PE=2 SV=2)

HSP 1 Score: 54.3 bits (129), Expect = 2.1e-06
Identity = 45/177 (25.42%), Postives = 82/177 (46.33%), Query Frame = 0


Query: 11  ISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLPAIVIDEAKRKLS 70
           +   E D+ V + +++++FK       PI + E+ + V +NY +     ++  EA   + 
Sbjct: 129 LPRSEIDEKVTDLVQFLLFKYQMKE--PITKAEILESVIRNYEDHF--PLLFSEASECML 188

Query: 71  SIFGYELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKYVEDVNTAHVTGF 130
            +FG +++E+  T                   S+VL++ L    Y   + DV +   TG 
Sbjct: 189 LVFGIDVKEVDPT-----------------GHSFVLVTSLGL-TYDGMLSDVQSMPKTGI 248

Query: 131 TFVIISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAI-ELVVQQRYLQ 187
             +I+SIV + G   PEE +   L  MGL +  E H + G  +  + +  VQ+ YL+
Sbjct: 249 LILILSIVFIEGYCTPEEVIWEALNMMGLYDGME-HLIYGEPRKLLTQDWVQENYLE 282

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BLAST of CSPI02G21050 vs. ExPASy Swiss-Prot
Match: P43355 (Melanoma-associated antigen 1 OS=Homo sapiens OX=9606 GN=MAGEA1 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 2.8e-06
Identity = 51/207 (24.64%), Postives = 91/207 (43.96%), Query Frame = 0


Query: 18  KLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLPAIVIDEAKRKLSSIFGYEL 77
           K VA+ + +++ K       P+ + E+ + V KNY++   P I   +A   L  +FG ++
Sbjct: 104 KKVADLVGFLLLKYRARE--PVTKAEMLESVIKNYKH-CFPEI-FGKASESLQLVFGIDV 163

Query: 78  RELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKYVEDVNTAHVTGFTFVIISI 137
           +E   T                   SYVL++ L    Y   + D      TGF  +++ +
Sbjct: 164 KEADPT-----------------GHSYVLVTCLGLS-YDGLLGDNQIMPKTGFLIIVLVM 223

Query: 138 VHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIELVVQQRYLQKVKVNAPEGNI 197
           + + GG  PEE +   L  M + +  E+       K   + +VQ++YL+  +V  P+ + 
Sbjct: 224 IAMEGGHAPEEEIWEELSVMEVYDGREHSAYGEPRKLLTQDLVQEKYLEYRQV--PDSDP 283

Query: 198 TFYEL--AERATYGPVSEKIKEYVAQI 223
             YE     RA       K+ EYV ++
Sbjct: 284 ARYEFLWGPRALAETSYVKVLEYVIKV 286

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BLAST of CSPI02G21050 vs. ExPASy Swiss-Prot
Match: P43361 (Melanoma-associated antigen 8 OS=Homo sapiens OX=9606 GN=MAGEA8 PE=1 SV=2)

HSP 1 Score: 53.9 bits (128), Expect = 2.8e-06
Identity = 53/212 (25.00%), Postives = 96/212 (45.28%), Query Frame = 0


Query: 14  EEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLPAIVIDEAKRKLSSIF 73
           E  D+ VAE +R+++ K       P+ + E+ + V KNY+N   P I   +A   +  IF
Sbjct: 110 EALDEKVAELVRFLLRKYQIKE--PVTKAEMLESVIKNYKNH-FPDI-FSKASECMQVIF 169

Query: 74  GYELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKYVEDVNTAHVTGFTFV 133
           G +++E+                      SY+L++ L    Y   + D  +   TG   +
Sbjct: 170 GIDVKEVDPA-----------------GHSYILVTCLGLS-YDGLLGDDQSTPKTGLLII 229

Query: 134 IISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAI-ELVVQQRYLQKVKVNA 193
           ++ ++ + G + PEE++   L  MGL +  E H V   ++  + +  VQ+ YL+  +  A
Sbjct: 230 VLGMILMEGSRAPEEAIWEALSVMGLYDGRE-HSVYWKLRKLLTQEWVQENYLEYRQ--A 289

Query: 194 PEGNITFYEL--AERATYGPVSEKIKEYVAQI 223
           P  +   YE     RA       K+ E+V ++
Sbjct: 290 PGSDPVRYEFLWGPRALAETSYVKVLEHVVRV 296

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BLAST of CSPI02G21050 vs. ExPASy TrEMBL
Match: A0A0A0LL83 (MAGE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G368960 PE=4 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.3e-123
Identity = 230/232 (99.14%), Postives = 231/232 (99.57%), Query Frame = 0


Query: 1   MASAGEYELDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLPAI 60
           MASAGEYELDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLPAI
Sbjct: 1   MASAGEYELDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLPAI 60

Query: 61  VIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKYVE 120
           VIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKYVE
Sbjct: 61  VIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKYVE 120

Query: 121 DVNTAHVTGFTFVIISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIELVV 180
           DVNTAHVTGFTFVIISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIELVV
Sbjct: 121 DVNTAHVTGFTFVIISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIELVV 180

Query: 181 QQRYLQKVKVNAPEGNITFYELAERATYGPVSEKIKEYVAQIVNSNVTAADT 233
           QQRYLQKVKVNAPEGNITFYELAERAT GPVSEKIKEYVAQIVN+NVTAADT
Sbjct: 181 QQRYLQKVKVNAPEGNITFYELAERATDGPVSEKIKEYVAQIVNTNVTAADT 232

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BLAST of CSPI02G21050 vs. ExPASy TrEMBL
Match: A0A1S3BBA9 (uncharacterized protein LOC103488065 OS=Cucumis melo OX=3656 GN=LOC103488065 PE=4 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 7.4e-119
Identity = 222/234 (94.87%), Postives = 229/234 (97.86%), Query Frame = 0


Query: 1   MASAGEY--ELDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP 60
           MASAGE+  +LDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP
Sbjct: 1   MASAGEHLSQLDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP 60

Query: 61  AIVIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKY 120
           AIVIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNV DAKSYVLISKLPADIYRKY
Sbjct: 61  AIVIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVADAKSYVLISKLPADIYRKY 120

Query: 121 VEDVNTAHVTGFTFVIISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIEL 180
           VEDV+TAHVTGFTFV++SIVHLAGGKIPEESLRHHL+RMGLSESDENHPVLGNIKHAIEL
Sbjct: 121 VEDVDTAHVTGFTFVVVSIVHLAGGKIPEESLRHHLRRMGLSESDENHPVLGNIKHAIEL 180

Query: 181 VVQQRYLQKVKVNAPEGNITFYELAERATYGPVSEKIKEYVAQIVNSNVTAADT 233
           VVQQRYLQKVKVNAPEGNITFYELAERAT GPVSEKIKEYVAQIVN+NVTA DT
Sbjct: 181 VVQQRYLQKVKVNAPEGNITFYELAERATDGPVSEKIKEYVAQIVNTNVTATDT 234

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BLAST of CSPI02G21050 vs. ExPASy TrEMBL
Match: A0A5A7VDL8 (Melanoma-associated antigen 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004830 PE=4 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 1.2e-113
Identity = 210/218 (96.33%), Postives = 215/218 (98.62%), Query Frame = 0


Query: 15  EKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLPAIVIDEAKRKLSSIFG 74
           EKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLPAIVIDEAKRKLSSIFG
Sbjct: 10  EKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLPAIVIDEAKRKLSSIFG 69

Query: 75  YELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKYVEDVNTAHVTGFTFVI 134
           YELRELQRTRPSSTGQPHSSQQNV DAKSYVLISKLPADIYRKYVEDV+TAHVTGFTFV+
Sbjct: 70  YELRELQRTRPSSTGQPHSSQQNVADAKSYVLISKLPADIYRKYVEDVDTAHVTGFTFVV 129

Query: 135 ISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIELVVQQRYLQKVKVNAPE 194
           +SIVHLAGGKIPEESLRHHL+RMGLSESDENHPVLGNIKHAIELVVQQRYLQKVKVNAPE
Sbjct: 130 VSIVHLAGGKIPEESLRHHLRRMGLSESDENHPVLGNIKHAIELVVQQRYLQKVKVNAPE 189

Query: 195 GNITFYELAERATYGPVSEKIKEYVAQIVNSNVTAADT 233
           GNITFYELAERAT GPVSEKIKEYVAQIVN+NVTA DT
Sbjct: 190 GNITFYELAERATDGPVSEKIKEYVAQIVNTNVTATDT 227

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BLAST of CSPI02G21050 vs. ExPASy TrEMBL
Match: A0A6J1BU01 (melanoma-associated antigen 1 OS=Momordica charantia OX=3673 GN=LOC111004771 PE=4 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 2.9e-107
Identity = 199/233 (85.41%), Postives = 213/233 (91.42%), Query Frame = 0


Query: 1   MASAGEY--ELDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP 60
           MA AG+Y  ++DIS EEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP
Sbjct: 1   MAGAGDYLSQIDISNEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP 60

Query: 61  AIVIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKY 120
           A+VI+EAK K+SSIFGYELRELQRTRPSST Q HSSQQNV D KSYVLISKLPADIYRKY
Sbjct: 61  AVVIEEAKEKMSSIFGYELRELQRTRPSSTSQGHSSQQNVADTKSYVLISKLPADIYRKY 120

Query: 121 VEDVNTAHVTGFTFVIISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIEL 180
           VEDVNTAHVTGFTFV++SIVHLAGGKIPEESL HHL+RMGLSESDE HPVLGNIK A+EL
Sbjct: 121 VEDVNTAHVTGFTFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVEL 180

Query: 181 VVQQRYLQKVKVNAPEGNITFYELAERATYGPVSEKIKEYVAQIVNSNVTAAD 232
           +VQ+RYLQK K+N PEGNITFYELAERA  GPVSE+IKEYVAQIVN NV   D
Sbjct: 181 LVQKRYLQKAKINGPEGNITFYELAERALDGPVSEEIKEYVAQIVNKNVDTVD 233

[Close]

BLAST of CSPI02G21050 vs. ExPASy TrEMBL
Match: A0A6J1KPM1 (melanoma-associated antigen 1 OS=Cucurbita maxima OX=3661 GN=LOC111496460 PE=4 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 2.0e-103
Identity = 192/233 (82.40%), Postives = 210/233 (90.13%), Query Frame = 0


Query: 1   MASAGEY--ELDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP 60
           MAS+G+Y  ++DIS EEKDKLVAE IRYIIFKTHQNSGCPIKREELTQ+VTKNYRNRGLP
Sbjct: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60

Query: 61  AIVIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKY 120
           AIVI+EAK KLSSIFGYELRELQR RPSST Q H  Q NV D+KSYVLISKLPA++YRKY
Sbjct: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120

Query: 121 VEDVNTAHVTGFTFVIISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIEL 180
           VEDV+TAHVTGF FV++SIVHLAGGKIPEESL HHL+RMGLSE+DE HPVLGNIK A+EL
Sbjct: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 180

Query: 181 VVQQRYLQKVKVNAPEGNITFYELAERATYGPVSEKIKEYVAQIVNSNVTAAD 232
           +VQQRYLQK K+N PEGNITFYELAERA  GPVSEKIKEYVAQIVN NVT  +
Sbjct: 181 LVQQRYLQKAKINGPEGNITFYELAERAIDGPVSEKIKEYVAQIVNKNVTTEE 233

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BLAST of CSPI02G21050 vs. NCBI nr
Match: XP_004152637.1 (uncharacterized protein LOC101222439 [Cucumis sativus] >KGN62695.1 hypothetical protein Csa_021862 [Cucumis sativus])

HSP 1 Score: 452.2 bits (1162), Expect = 2.7e-123
Identity = 230/232 (99.14%), Postives = 231/232 (99.57%), Query Frame = 0


Query: 1   MASAGEYELDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLPAI 60
           MASAGEYELDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLPAI
Sbjct: 1   MASAGEYELDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLPAI 60

Query: 61  VIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKYVE 120
           VIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKYVE
Sbjct: 61  VIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKYVE 120

Query: 121 DVNTAHVTGFTFVIISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIELVV 180
           DVNTAHVTGFTFVIISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIELVV
Sbjct: 121 DVNTAHVTGFTFVIISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIELVV 180

Query: 181 QQRYLQKVKVNAPEGNITFYELAERATYGPVSEKIKEYVAQIVNSNVTAADT 233
           QQRYLQKVKVNAPEGNITFYELAERAT GPVSEKIKEYVAQIVN+NVTAADT
Sbjct: 181 QQRYLQKVKVNAPEGNITFYELAERATDGPVSEKIKEYVAQIVNTNVTAADT 232

[Close]

BLAST of CSPI02G21050 vs. NCBI nr
Match: XP_008444839.1 (PREDICTED: uncharacterized protein LOC103488065 [Cucumis melo] >XP_008444840.1 PREDICTED: uncharacterized protein LOC103488065 [Cucumis melo])

HSP 1 Score: 436.4 bits (1121), Expect = 1.5e-118
Identity = 222/234 (94.87%), Postives = 229/234 (97.86%), Query Frame = 0


Query: 1   MASAGEY--ELDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP 60
           MASAGE+  +LDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP
Sbjct: 1   MASAGEHLSQLDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP 60

Query: 61  AIVIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKY 120
           AIVIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNV DAKSYVLISKLPADIYRKY
Sbjct: 61  AIVIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVADAKSYVLISKLPADIYRKY 120

Query: 121 VEDVNTAHVTGFTFVIISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIEL 180
           VEDV+TAHVTGFTFV++SIVHLAGGKIPEESLRHHL+RMGLSESDENHPVLGNIKHAIEL
Sbjct: 121 VEDVDTAHVTGFTFVVVSIVHLAGGKIPEESLRHHLRRMGLSESDENHPVLGNIKHAIEL 180

Query: 181 VVQQRYLQKVKVNAPEGNITFYELAERATYGPVSEKIKEYVAQIVNSNVTAADT 233
           VVQQRYLQKVKVNAPEGNITFYELAERAT GPVSEKIKEYVAQIVN+NVTA DT
Sbjct: 181 VVQQRYLQKVKVNAPEGNITFYELAERATDGPVSEKIKEYVAQIVNTNVTATDT 234

[Close]

BLAST of CSPI02G21050 vs. NCBI nr
Match: XP_038884588.1 (melanoma-associated antigen 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 419.5 bits (1077), Expect = 1.9e-113
Identity = 213/234 (91.03%), Postives = 225/234 (96.15%), Query Frame = 0


Query: 1   MASAGEY--ELDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP 60
           MASAGEY  ++DISEEEKDKLVAEAIRYIIFKTHQ+SGCPIKREELTQLVTKNYRNRGLP
Sbjct: 1   MASAGEYLSQMDISEEEKDKLVAEAIRYIIFKTHQSSGCPIKREELTQLVTKNYRNRGLP 60

Query: 61  AIVIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKY 120
           A+VIDEAK+KLSSIFGYELRELQRTR SSTGQ HSSQQNV DAKSYVLISKLPADIYR+Y
Sbjct: 61  AVVIDEAKKKLSSIFGYELRELQRTRLSSTGQAHSSQQNVADAKSYVLISKLPADIYRRY 120

Query: 121 VEDVNTAHVTGFTFVIISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIEL 180
           VEDVNTAH+TGFTFV++SIVHLAGGKIPEESL HHL+RMGLSESDENHPVLGNIKHA EL
Sbjct: 121 VEDVNTAHMTGFTFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDENHPVLGNIKHAAEL 180

Query: 181 VVQQRYLQKVKVNAPEGNITFYELAERATYGPVSEKIKEYVAQIVNSNVTAADT 233
           VVQQRYLQKVKVNAPEGNITFYELAERA  GPVSEKIKEYVAQIVN+NVTAAD+
Sbjct: 181 VVQQRYLQKVKVNAPEGNITFYELAERAIDGPVSEKIKEYVAQIVNTNVTAADS 234

[Close]

BLAST of CSPI02G21050 vs. NCBI nr
Match: KAA0065147.1 (melanoma-associated antigen 8 [Cucumis melo var. makuwa])

HSP 1 Score: 419.1 bits (1076), Expect = 2.5e-113
Identity = 210/218 (96.33%), Postives = 215/218 (98.62%), Query Frame = 0


Query: 15  EKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLPAIVIDEAKRKLSSIFG 74
           EKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLPAIVIDEAKRKLSSIFG
Sbjct: 10  EKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLPAIVIDEAKRKLSSIFG 69

Query: 75  YELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKYVEDVNTAHVTGFTFVI 134
           YELRELQRTRPSSTGQPHSSQQNV DAKSYVLISKLPADIYRKYVEDV+TAHVTGFTFV+
Sbjct: 70  YELRELQRTRPSSTGQPHSSQQNVADAKSYVLISKLPADIYRKYVEDVDTAHVTGFTFVV 129

Query: 135 ISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIELVVQQRYLQKVKVNAPE 194
           +SIVHLAGGKIPEESLRHHL+RMGLSESDENHPVLGNIKHAIELVVQQRYLQKVKVNAPE
Sbjct: 130 VSIVHLAGGKIPEESLRHHLRRMGLSESDENHPVLGNIKHAIELVVQQRYLQKVKVNAPE 189

Query: 195 GNITFYELAERATYGPVSEKIKEYVAQIVNSNVTAADT 233
           GNITFYELAERAT GPVSEKIKEYVAQIVN+NVTA DT
Sbjct: 190 GNITFYELAERATDGPVSEKIKEYVAQIVNTNVTATDT 227

[Close]

BLAST of CSPI02G21050 vs. NCBI nr
Match: XP_038884587.1 (uncharacterized protein LOC120075353 isoform X1 [Benincasa hispida])

HSP 1 Score: 411.4 bits (1056), Expect = 5.3e-111
Identity = 213/244 (87.30%), Postives = 225/244 (92.21%), Query Frame = 0


Query: 1   MASAGEY--ELDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP 60
           MASAGEY  ++DISEEEKDKLVAEAIRYIIFKTHQ+SGCPIKREELTQLVTKNYRNRGLP
Sbjct: 1   MASAGEYLSQMDISEEEKDKLVAEAIRYIIFKTHQSSGCPIKREELTQLVTKNYRNRGLP 60

Query: 61  AIVIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKY 120
           A+VIDEAK+KLSSIFGYELRELQRTR SSTGQ HSSQQNV DAKSYVLISKLPADIYR+Y
Sbjct: 61  AVVIDEAKKKLSSIFGYELRELQRTRLSSTGQAHSSQQNVADAKSYVLISKLPADIYRRY 120

Query: 121 VEDVNTAHVTGFTFVIISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIEL 180
           VEDVNTAH+TGFTFV++SIVHLAGGKIPEESL HHL+RMGLSESDENHPVLGNIKHA EL
Sbjct: 121 VEDVNTAHMTGFTFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDENHPVLGNIKHAAEL 180

Query: 181 VVQQ----------RYLQKVKVNAPEGNITFYELAERATYGPVSEKIKEYVAQIVNSNVT 233
           VVQQ          RYLQKVKVNAPEGNITFYELAERA  GPVSEKIKEYVAQIVN+NVT
Sbjct: 181 VVQQRCLFTLALLCRYLQKVKVNAPEGNITFYELAERAIDGPVSEKIKEYVAQIVNTNVT 240

[Close]

BLAST of CSPI02G21050 vs. TAIR 10
Match: AT1G34770.1 (CONTAINS InterPro DOMAIN/s: MAGE protein (InterPro:IPR002190); Has 1274 Blast hits to 1260 proteins in 85 species: Archae - 0; Bacteria - 0; Metazoa - 1104; Fungi - 45; Plants - 49; Viruses - 0; Other Eukaryotes - 76 (source: NCBI BLink). )

HSP 1 Score: 276.2 bits (705), Expect = 2.5e-74
Identity = 136/225 (60.44%), Postives = 181/225 (80.44%), Query Frame = 0


Query: 8   ELDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLPAIVIDEAKR 67
           + DIS+EE DKLV+E IR+I+FK HQ+SG PIKRE+LTQ+VTKNYR R L   VI+EAK+
Sbjct: 10  QFDISKEETDKLVSEVIRFILFKFHQSSGTPIKREDLTQIVTKNYRQRNLATHVINEAKK 69

Query: 68  KLSSIFGYELRELQRTRPSSTGQPHSSQ-QNVGDAKSYVLISKLPADIYRKYVEDVNTAH 127
           KLS++FGY+L+ELQR R SSTGQ    Q Q+  D+KSYVL+S+LP ++++K+V D  T+ 
Sbjct: 70  KLSNVFGYDLKELQRARSSSTGQSRLPQSQSSVDSKSYVLVSELPLEVFKKHVVDETTSP 129

Query: 128 VTGFTFVIISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIELVVQQRYLQ 187
           VTGFTFV+++IV LAGGKIPEE+L HHLKRMGL E+DE++PV GN K  +E +VQQR+LQ
Sbjct: 130 VTGFTFVVLAIVQLAGGKIPEETLWHHLKRMGLHENDEHNPVFGNNKQTLETLVQQRFLQ 189

Query: 188 KVKVNAPEGNITFYELAERATYGPVSEKIKEYVAQIVNSNVTAAD 232
           K KV+ PEG+  FY+LAERA    VSEK+K+Y++QI+ ++V   +
Sbjct: 190 KEKVSGPEGSTLFYDLAERALDPQVSEKVKDYISQILKNDVAVVE 234

The following BLAST results are available for this feature:

BLAST of CSPI02G21050 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43363	2.1e-06	25.42	Melanoma-associated antigen 10 OS=Homo sapiens OX=9606 GN=MAGEA10 PE=2 SV=2	[more]
P43355	2.8e-06	24.64	Melanoma-associated antigen 1 OS=Homo sapiens OX=9606 GN=MAGEA1 PE=1 SV=1	[more]
P43361	2.8e-06	25.00	Melanoma-associated antigen 8 OS=Homo sapiens OX=9606 GN=MAGEA8 PE=1 SV=2	[more]

BLAST of CSPI02G21050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LL83	1.3e-123	99.14	MAGE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G368960 PE=4 S...	[more]
A0A1S3BBA9	7.4e-119	94.87	uncharacterized protein LOC103488065 OS=Cucumis melo OX=3656 GN=LOC103488065 PE=...	[more]
A0A5A7VDL8	1.2e-113	96.33	Melanoma-associated antigen 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc...	[more]
A0A6J1BU01	2.9e-107	85.41	melanoma-associated antigen 1 OS=Momordica charantia OX=3673 GN=LOC111004771 PE=...	[more]
A0A6J1KPM1	2.0e-103	82.40	melanoma-associated antigen 1 OS=Cucurbita maxima OX=3661 GN=LOC111496460 PE=4 S...	[more]

BLAST of CSPI02G21050 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152637.1	2.7e-123	99.14	uncharacterized protein LOC101222439 [Cucumis sativus] >KGN62695.1 hypothetical ...	[more]
XP_008444839.1	1.5e-118	94.87	PREDICTED: uncharacterized protein LOC103488065 [Cucumis melo] >XP_008444840.1 P...	[more]
XP_038884588.1	1.9e-113	91.03	melanoma-associated antigen 1 isoform X2 [Benincasa hispida]	[more]
KAA0065147.1	2.5e-113	96.33	melanoma-associated antigen 8 [Cucumis melo var. makuwa]	[more]
XP_038884587.1	5.3e-111	87.30	uncharacterized protein LOC120075353 isoform X1 [Benincasa hispida]	[more]

BLAST of CSPI02G21050 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G34770.1	2.5e-74	60.44	CONTAINS InterPro DOMAIN/s: MAGE protein (InterPro:IPR002190); Has 1274 Blast hi...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002190	MAGE homology domain	SMART	SM01373	MAGE_2	coord: 23..215 e-value: 4.1E-24 score: 96.1
IPR002190	MAGE homology domain	PFAM	PF01454	MAGE	coord: 24..207 e-value: 2.5E-38 score: 131.9
IPR002190	MAGE homology domain	PROSITE	PS50838	MAGE	coord: 38..232 score: 13.528666
IPR041898	MAGE homology domain, winged helix WH1 motif	GENE3D	1.10.10.1200	MAGE homology domain, winged helix WH1 motif	coord: 10..112 e-value: 4.0E-22 score: 79.9
IPR041899	MAGE homology domain, winged helix WH2 motif	GENE3D	1.10.10.1210	MAGE homology domain, winged helix WH2 motif	coord: 116..232 e-value: 3.4E-20 score: 74.0
None	No IPR available	PANTHER	PTHR11736:SF14	MAGE PROTEIN	coord: 7..229
IPR037445	Melanoma-associated antigen	PANTHER	PTHR11736	MELANOMA-ASSOCIATED ANTIGEN MAGE ANTIGEN	coord: 7..229

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G21050.1	CSPI02G21050.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005634	nucleus