CSPI02G21030 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G21030
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionAB hydrolase-1 domain-containing protein
LocationChr2: 18521409 .. 18523414 (+)
RNA-Seq ExpressionCSPI02G21030
SyntenyCSPI02G21030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTTATCACGTTCAAAAGCCCATAATTGCCTTGCAATTTCTCAAAACAGAGTCCCCATTAATTCAAACTCTGATTCTGAGAAGATTCTTTCATGTTCTTCAAATGACAAAGTGTTTCTTCTTCAGTATCGTAGAGACCAAAAATTGGTTCCATCGTTATAGCTTCACCAAATCCGGTCTCCGATCCACCATTACAGATCTCAAAGACGGCACCACAGTTCATTGTTGGGTTCCCAAAAACCCAATTCACACCAAACCCAATCTCCTTTTAATCCATGGTATTGGAGCCAATGCACTTTGGCAATGGGGTGATTTCATTCCAGCTCTCATACCTTATTTCAATCTCTACATCCCTGATTTGATCTTCTTCGGCGACTCGTTTACCACTCAGCCAGACCGGACTGAGTGGTTTCAAGCTCAGTGTCTAATACGAGTCATGGAGGCTAACACGGTGGGGAAATTCAGTTTGGTGGGGCTTAGTTATGGAGGATTTGTTGGGTACAGCATCGCGGCGCTGCGGCCTGAAATGGTGGAGAGAGTAGTGATTTGTTGTTCTGGGGTTTGTGTGGAAGAGAAGGATTTTAAAGATGGGTTGTTGAAAGTTTCTGCTTTGGAGGATGCTACAGCCATTTTGGTTCCCCAAAAGCCAGAGAAGCTTAAACAGCTAGTGGGTTACTCCTTTTTCCGGCCGCCGCCGTTGCGGCTGATACCTTCTTGTTTGCTCAATGATTTCATTGAAGTAAGGCTTGTCGTAGTATTTGTGATTCTTCCATCACATCTTTTTTATTTGAAAATTTCCTTCCTTTTTGTGTGTTAATTTGACTAGTTTATCTTTGTGTTTGTGTGGCAGTCAATGTGTTTAGATCATATTGAGGAAAAGAGAGAGTTGATTCGAACTATTCCCAGAGGAAGAAAGCTTTCCGACCTTCCAAAGATTCAACAGGTGGGATTGCTATTCAACATAACTTATCCATATTAGCTTTAAACAGATCATTAATTACTTGTCTTCATTGCCATTCCATTCTACTCTCTGTTCTTCATAATGTTCAAATAGCTTAGCATTTTTACACCCGAGTCTAAGTCCTTCGAATATTGTTAAAAATTTATTGATGCTATTAGACATTCTATTAGATACTTTTGAGATCGAATCAAACAGACAAAAAAACTCCAATCTCAAAAACTAAACTTGTAGTTTAACATTTTTCTGACTTGCTTGCAGCGGACGATGATAATGTGGGGAGAACATGATCAAGTATTTCCATTAGAATTGGGTCACAGGTTGAAAAGGTGAGTTAATCAAGCTCCTAAAAGCAAACAATGCATCAAATATTCTCATTTTGAACCAAATGATAAATGTGTTGGCCACTCCTTTGAAGTAGGCATTTGGGAGACAATGCTACGCTTGTTGTTATCAAGAATACAGGCCATGCCTTCAATTCAGAGGAGCCAAAGGAGTTTCTCAGCCATTTGATTTCATTCCTAGTTGATCCATAGCCTCCTCTCCTCATCAGTCAATGAACGTCAAGCAGTTCAAACGCACAAACAACATCATCAAGAAACAGATAATGAGCAACGTCAAAAGCTGGAGTGATTTAATGATCGTTAATAGTACAATAACAAACAAGAGAAGAAATATACCTCAGCCATAGCTCAATTGGTTCATTCGTCCAAATCTAATGTTGAAAATACATTAAAACAATTAAATTTGTTATAGAAATTTCTGACATTTTCCATTTTGACCCTTTTGTTCCGAGTTCTAGATTCAGAAATCTTTAACCATAAAATCTGTGTTCCTAAGTACTCCTCAACCAACAAG

mRNA sequence

GCTTATCACGTTCAAAAGCCCATAATTGCCTTGCAATTTCTCAAAACAGAGTCCCCATTAATTCAAACTCTGATTCTGAGAAGATTCTTTCATGTTCTTCAAATGACAAAGTGTTTCTTCTTCAGTATCGTAGAGACCAAAAATTGGTTCCATCGTTATAGCTTCACCAAATCCGGTCTCCGATCCACCATTACAGATCTCAAAGACGGCACCACAGTTCATTGTTGGGTTCCCAAAAACCCAATTCACACCAAACCCAATCTCCTTTTAATCCATGGTATTGGAGCCAATGCACTTTGGCAATGGGGTGATTTCATTCCAGCTCTCATACCTTATTTCAATCTCTACATCCCTGATTTGATCTTCTTCGGCGACTCGTTTACCACTCAGCCAGACCGGACTGAGTGGTTTCAAGCTCAGTGTCTAATACGAGTCATGGAGGCTAACACGGTGGGGAAATTCAGTTTGGTGGGGCTTAGTTATGGAGGATTTGTTGGGTACAGCATCGCGGCGCTGCGGCCTGAAATGGTGGAGAGAGTAGTGATTTGTTGTTCTGGGGTTTGTGTGGAAGAGAAGGATTTTAAAGATGGGTTGTTGAAAGTTTCTGCTTTGGAGGATGCTACAGCCATTTTGGTTCCCCAAAAGCCAGAGAAGCTTAAACAGCTAGTGGGTTACTCCTTTTTCCGGCCGCCGCCGTTGCGGCTGATACCTTCTTGTTTGCTCAATGATTTCATTGAATCAATGTGTTTAGATCATATTGAGGAAAAGAGAGAGTTGATTCGAACTATTCCCAGAGGAAGAAAGCTTTCCGACCTTCCAAAGATTCAACAGCGGACGATGATAATGTGGGGAGAACATGATCAAGTATTTCCATTAGAATTGGGTCACAGGTTGAAAAGGCATTTGGGAGACAATGCTACGCTTGTTGTTATCAAGAATACAGGCCATGCCTTCAATTCAGAGGAGCCAAAGGAGTTTCTCAGCCATTTGATTTCATTCCTAGTTGATCCATAGCCTCCTCTCCTCATCAGTCAATGAACGTCAAGCAGTTCAAACGCACAAACAACATCATCAAGAAACAGATAATGAGCAACGTCAAAAGCTGGAGTGATTTAATGATCGTTAATAGTACAATAACAAACAAGAGAAGAAATATACCTCAGCCATAGCTCAATTGGTTCATTCGTCCAAATCTAATGTTGAAAATACATTAAAACAATTAAATTTGTTATAGAAATTTCTGACATTTTCCATTTTGACCCTTTTGTTCCGAGTTCTAGATTCAGAAATCTTTAACCATAAAATCTGTGTTCCTAAGTACTCCTCAACCAACAAG

Coding sequence (CDS)

ATGACAAAGTGTTTCTTCTTCAGTATCGTAGAGACCAAAAATTGGTTCCATCGTTATAGCTTCACCAAATCCGGTCTCCGATCCACCATTACAGATCTCAAAGACGGCACCACAGTTCATTGTTGGGTTCCCAAAAACCCAATTCACACCAAACCCAATCTCCTTTTAATCCATGGTATTGGAGCCAATGCACTTTGGCAATGGGGTGATTTCATTCCAGCTCTCATACCTTATTTCAATCTCTACATCCCTGATTTGATCTTCTTCGGCGACTCGTTTACCACTCAGCCAGACCGGACTGAGTGGTTTCAAGCTCAGTGTCTAATACGAGTCATGGAGGCTAACACGGTGGGGAAATTCAGTTTGGTGGGGCTTAGTTATGGAGGATTTGTTGGGTACAGCATCGCGGCGCTGCGGCCTGAAATGGTGGAGAGAGTAGTGATTTGTTGTTCTGGGGTTTGTGTGGAAGAGAAGGATTTTAAAGATGGGTTGTTGAAAGTTTCTGCTTTGGAGGATGCTACAGCCATTTTGGTTCCCCAAAAGCCAGAGAAGCTTAAACAGCTAGTGGGTTACTCCTTTTTCCGGCCGCCGCCGTTGCGGCTGATACCTTCTTGTTTGCTCAATGATTTCATTGAATCAATGTGTTTAGATCATATTGAGGAAAAGAGAGAGTTGATTCGAACTATTCCCAGAGGAAGAAAGCTTTCCGACCTTCCAAAGATTCAACAGCGGACGATGATAATGTGGGGAGAACATGATCAAGTATTTCCATTAGAATTGGGTCACAGGTTGAAAAGGCATTTGGGAGACAATGCTACGCTTGTTGTTATCAAGAATACAGGCCATGCCTTCAATTCAGAGGAGCCAAAGGAGTTTCTCAGCCATTTGATTTCATTCCTAGTTGATCCATAG

Protein sequence

MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGIGANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKFSLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQKPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPKIQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFLVDP*
Homology
BLAST of CSPI02G21030 vs. ExPASy Swiss-Prot
Match: O05235 (Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yugF PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 2.6e-12
Identity = 70/277 (25.27%), Postives = 120/277 (43.32%), Query Frame = 0

Query: 35  DGTTVHCWVPKNPIHTKPNLLLIHGIGANALWQWGDFIPALIPYFNLYIPDLIFFGDSFT 94
           DG  V+    +NP   +  L+ +HG  ++A + +   IP L   +++   DL  FG S  
Sbjct: 13  DGVNVYYEHYQNP--GRQTLVCVHGFLSSA-FSFRKVIPLLRDKYDIIALDLPPFGQS-- 72

Query: 95  TQPDRTEWFQ----AQCLIRVMEANTVGKFSLVGLSYGGFVGYSIAALRPEMVERVVICC 154
            +  RT  +     A+ +I ++E   V +  LVG S GG +  S A  +PE+  +VV+ C
Sbjct: 73  -EKSRTFIYTYQNLAKLVIGILEHLQVKQAVLVGHSMGGQISLSAALQKPELFSKVVLLC 132

Query: 155 SGVCVEEKDFKDGLLKVSALEDATAILVPQKPEKLKQLVGYSFFRPPPLRLI--PSCLLN 214
           S           G LK S         +P     +K+ +         L ++   S +  
Sbjct: 133 S----------SGYLKRSHPTIIFGTHIPYFHLYIKRWLSKEGVMKNLLNVVHDKSLIDE 192

Query: 215 DFIESMCLDHIEEK-----RELIRTIPRGRKLSDLPKIQQRTMIMWGEHDQVFPLELGHR 274
           + I+       +E+        IR      +   L K+ +  +++WGE D++ P+E+G R
Sbjct: 193 EMIDGYGRPFQDEQIFKAMTRFIRHREGDLEPEQLKKMNKPALLIWGEEDRIVPMEIGKR 252

Query: 275 LKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFL 301
           L   L  N+ L  +  TGH    E P+    H+  F+
Sbjct: 253 LHADL-PNSVLYSLGQTGHLVPEERPELISEHIADFI 272

BLAST of CSPI02G21030 vs. ExPASy Swiss-Prot
Match: P91143 (Abhydrolase domain-containing protein abhd-5.1 OS=Caenorhabditis elegans OX=6239 GN=abhd-5.1 PE=3 SV=2)

HSP 1 Score: 62.0 bits (149), Expect = 1.3e-08
Identity = 36/115 (31.30%), Postives = 59/115 (51.30%), Query Frame = 0

Query: 38  TVHCWVPKNPIHTKPNLLLIHGIGANALWQWGDFIPALIPYFNLYIPDLIFFGDS----F 97
           TV+C    + + +K  ++LIHG GA     WG  I  L  + N+Y  DL  FG S    F
Sbjct: 58  TVNCENESSELKSKYPIVLIHGFGAGVA-LWGSAIKRLAQFQNVYAIDLPGFGRSSRTKF 117

Query: 98  TTQPDRTEWFQAQCLIRVMEANTVGKFSLVGLSYGGFVGYSIAALRPEMVERVVI 149
           +T P+  E    + + +      + K +LVG S+GG++  S A   P+ +E +++
Sbjct: 118 STDPETAEKEMIEAIEQWRVKMNLEKMNLVGHSFGGYLSTSYALKYPKRIENLIL 171

BLAST of CSPI02G21030 vs. ExPASy Swiss-Prot
Match: Q5F641 (Pimeloyl-[acyl-carrier protein] methyl ester esterase OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) OX=242231 GN=bioH PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 2.3e-08
Identity = 63/245 (25.71%), Postives = 96/245 (39.18%), Query Frame = 0

Query: 56  LIHGIGANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEAN 115
           LIHG GAN    + D +P L   + +   DL   GD+   QP     F  +     + A 
Sbjct: 19  LIHGWGANR-HAFDDLMPRLPATWPVSAVDLPGHGDAPFAQP-----FDIEAAADGIAAQ 78

Query: 116 TVGKFSLVGLSYGGFVGYSIAALRPEMVERVVICCS-GVCVEEKDFKDGLLKVSALEDAT 175
                 ++G S GG V   +AA  P+ V  + +  S       +D+ +G L   AL    
Sbjct: 79  IDTSADILGWSLGGLVALYLAARHPDKVRSLCLTASFARLTAAEDYPEG-LAAPALGKMV 138

Query: 176 AILVPQKPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRK 235
                   + +KQ +       P    I   +L D         ++   E +    R   
Sbjct: 139 GAFRTDYAKHIKQFLQLQLLHTPDAAEIIGRILPDLARCGTPQALQ---EALDAAERADA 198

Query: 236 LSDLPKIQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLS 295
              L KI    ++++G  D + PL +G  L RHL   + LVV++   HA        FLS
Sbjct: 199 RHLLDKIDVPVLLVFGGKDAITPLRMGEYLHRHL-KGSRLVVMEKAAHA-------PFLS 245

Query: 296 HLISF 300
           H  +F
Sbjct: 259 HAEAF 245

BLAST of CSPI02G21030 vs. ExPASy Swiss-Prot
Match: P9WNH2 (Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT2788 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 2.3e-08
Identity = 57/263 (21.67%), Postives = 110/263 (41.83%), Query Frame = 0

Query: 52  PNLLLIHGIGANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRV 111
           P +LLIHGIG N+   W      L   F +  PDL+  G S   + D +    A  +  +
Sbjct: 38  PAILLIHGIGDNST-TWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 112 MEANTVGKFSLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALE 171
           +    + + ++VG S GG V    A   P++V+R+++  +G   ++ +    L  +    
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVSAGGVTKDVNIVFRLASLPMGS 157

Query: 172 DATAI----LVPQKPEKLKQLVGYSFFRPPPLRLIPSCL--LNDFIESMCLDH------- 231
           +A A+    LV    +   ++VG +         +P+ L  L+D  E             
Sbjct: 158 EAMALLRLPLVLPAVQIAGRIVGKAIGTTSLGHDLPNVLRILDDLPEPTASAAFGRTLRA 217

Query: 232 -IEEKRELIRTIPRGRKLSDLPKIQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVI 291
            ++ + +++  + R      +P       I+WG  D V P+   H     +   + L + 
Sbjct: 218 VVDWRGQMVTMLDRCYLTEAIP-----VQIIWGTKDVVLPVRHAHMAHAAM-PGSQLEIF 277

Query: 292 KNTGHAFNSEEPKEFLSHLISFL 301
           + +GH    ++P  F+  +  F+
Sbjct: 278 EGSGHFPFHDDPARFIDIVERFM 293

BLAST of CSPI02G21030 vs. ExPASy Swiss-Prot
Match: P9WNH3 (Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv2715 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 2.3e-08
Identity = 57/263 (21.67%), Postives = 110/263 (41.83%), Query Frame = 0

Query: 52  PNLLLIHGIGANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRV 111
           P +LLIHGIG N+   W      L   F +  PDL+  G S   + D +    A  +  +
Sbjct: 38  PAILLIHGIGDNST-TWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 112 MEANTVGKFSLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALE 171
           +    + + ++VG S GG V    A   P++V+R+++  +G   ++ +    L  +    
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVSAGGVTKDVNIVFRLASLPMGS 157

Query: 172 DATAI----LVPQKPEKLKQLVGYSFFRPPPLRLIPSCL--LNDFIESMCLDH------- 231
           +A A+    LV    +   ++VG +         +P+ L  L+D  E             
Sbjct: 158 EAMALLRLPLVLPAVQIAGRIVGKAIGTTSLGHDLPNVLRILDDLPEPTASAAFGRTLRA 217

Query: 232 -IEEKRELIRTIPRGRKLSDLPKIQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVI 291
            ++ + +++  + R      +P       I+WG  D V P+   H     +   + L + 
Sbjct: 218 VVDWRGQMVTMLDRCYLTEAIP-----VQIIWGTKDVVLPVRHAHMAHAAM-PGSQLEIF 277

Query: 292 KNTGHAFNSEEPKEFLSHLISFL 301
           + +GH    ++P  F+  +  F+
Sbjct: 278 EGSGHFPFHDDPARFIDIVERFM 293

BLAST of CSPI02G21030 vs. ExPASy TrEMBL
Match: A0A0A0LLN7 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G368940 PE=4 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.2e-179
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0

Query: 1   MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 60
           MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI
Sbjct: 1   MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 60

Query: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 120
           GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF
Sbjct: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 120

Query: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 180
           SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 180

Query: 181 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240
           KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 241 IQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFL 300
           IQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFL
Sbjct: 241 IQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFL 300

Query: 301 VDP 304
           VDP
Sbjct: 301 VDP 303

BLAST of CSPI02G21030 vs. ExPASy TrEMBL
Match: A0A5A7VI51 (2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2, 4-dienoic acid hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004850 PE=4 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 2.8e-174
Identity = 293/303 (96.70%), Postives = 299/303 (98.68%), Query Frame = 0

Query: 1   MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 60
           MTKC FFSI ETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPI TKPNLLLIHGI
Sbjct: 1   MTKCLFFSITETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIRTKPNLLLIHGI 60

Query: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 120
           GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEA+TVGKF
Sbjct: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEAHTVGKF 120

Query: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 180
           SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVS LE+ATAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSDLEEATAILVPQ 180

Query: 181 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240
           KPEKLKQLVGYSFFRPPPLRL+PSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPLRLLPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 241 IQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFL 300
           IQQRT+IMWGEHDQVFP+ELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFL+HLISFL
Sbjct: 241 IQQRTLIMWGEHDQVFPVELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLNHLISFL 300

Query: 301 VDP 304
           VDP
Sbjct: 301 VDP 303

BLAST of CSPI02G21030 vs. ExPASy TrEMBL
Match: A0A1S3BC20 (LOW QUALITY PROTEIN: 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase OS=Cucumis melo OX=3656 GN=LOC103488063 PE=4 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 1.8e-173
Identity = 292/303 (96.37%), Postives = 298/303 (98.35%), Query Frame = 0

Query: 1   MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 60
           MTKC FFSI ETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPI TKPNLLLIHGI
Sbjct: 1   MTKCLFFSITETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIRTKPNLLLIHGI 60

Query: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 120
           GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEA+TVGKF
Sbjct: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEAHTVGKF 120

Query: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 180
           SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVS LE+ATAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSDLEEATAILVPQ 180

Query: 181 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240
           KPEKLKQLVGYSF RPPPLRL+PSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFXRPPPLRLLPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 241 IQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFL 300
           IQQRT+IMWGEHDQVFP+ELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFL+HLISFL
Sbjct: 241 IQQRTLIMWGEHDQVFPVELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLNHLISFL 300

Query: 301 VDP 304
           VDP
Sbjct: 301 VDP 303

BLAST of CSPI02G21030 vs. ExPASy TrEMBL
Match: A0A6J1HDR2 (uncharacterized protein LOC111462585 OS=Cucurbita moschata OX=3662 GN=LOC111462585 PE=4 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 1.1e-159
Identity = 260/301 (86.38%), Postives = 287/301 (95.35%), Query Frame = 0

Query: 1   MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 60
           MT+CFFFSI ETKNW+HR+ FTK GLRSTITDL DGTTVHCWVPKNP+H+KPNLLLIHGI
Sbjct: 1   MTRCFFFSITETKNWYHRHRFTKCGLRSTITDLNDGTTVHCWVPKNPLHSKPNLLLIHGI 60

Query: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 120
           GANALWQWGD I  L+P+FNLY+PDLIFFGDSF+T PDRTEWFQAQCLIRVMEAN+VGK 
Sbjct: 61  GANALWQWGDSIATLVPHFNLYVPDLIFFGDSFSTNPDRTEWFQAQCLIRVMEANSVGKL 120

Query: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 180
           SLVGLSYGGFVGYS+AAL P+MVE+VVICC+GVCVEEKDFKDGLL+VS LE+A AILVPQ
Sbjct: 121 SLVGLSYGGFVGYSMAALCPDMVEKVVICCAGVCVEEKDFKDGLLRVSDLEEAIAILVPQ 180

Query: 181 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240
           KPEKLKQLVGYSFFRPPP+RL+PSCLLNDFIESMCLDHI+EKRELIRTIP+GRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPMRLMPSCLLNDFIESMCLDHIDEKRELIRTIPKGRKLSDLPK 240

Query: 241 IQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFL 300
           I QRTMI+WGEHD+VFPLELGHRLKRHLGDNATLV++KN GHAFNSEEPKEFL+HL+SFL
Sbjct: 241 IHQRTMILWGEHDEVFPLELGHRLKRHLGDNATLVIVKNAGHAFNSEEPKEFLNHLVSFL 300

Query: 301 V 302
           +
Sbjct: 301 L 301

BLAST of CSPI02G21030 vs. ExPASy TrEMBL
Match: A0A6J1KAV5 (uncharacterized protein LOC111492191 OS=Cucurbita maxima OX=3661 GN=LOC111492191 PE=4 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 1.6e-158
Identity = 259/301 (86.05%), Postives = 285/301 (94.68%), Query Frame = 0

Query: 1   MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 60
           MT+CFFFSI ETKNW+HR+ FTK GLRSTITDL DGTTVHCWVPKNP+H+KPNLLLIHGI
Sbjct: 1   MTRCFFFSITETKNWYHRHRFTKCGLRSTITDLNDGTTVHCWVPKNPLHSKPNLLLIHGI 60

Query: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 120
           GANALWQWGD I  L+P+FNLY+PDLIFFGDS TT PDRTEWFQAQCLIRVMEAN+VGK 
Sbjct: 61  GANALWQWGDSIATLVPHFNLYVPDLIFFGDSSTTNPDRTEWFQAQCLIRVMEANSVGKL 120

Query: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 180
           SLVGLSYGGFVGYS+AAL P+MVE+VVICC+G+CVEEKDFKDGLL+VS LE+A AILVPQ
Sbjct: 121 SLVGLSYGGFVGYSMAALCPDMVEKVVICCAGICVEEKDFKDGLLRVSDLEEAIAILVPQ 180

Query: 181 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240
           KPEKLKQLVGYSFFRPPP+RL+PSCLLNDFIESMCLDHI+EKRELIRTIP+GRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPMRLMPSCLLNDFIESMCLDHIDEKRELIRTIPKGRKLSDLPK 240

Query: 241 IQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFL 300
           I  RTMI+WGEHD++FPLELGHRLKRHLGDNATLV+IKN GHAFNSEEPKEFL+HLISFL
Sbjct: 241 IHPRTMILWGEHDEIFPLELGHRLKRHLGDNATLVIIKNAGHAFNSEEPKEFLNHLISFL 300

Query: 301 V 302
           +
Sbjct: 301 L 301

BLAST of CSPI02G21030 vs. NCBI nr
Match: XP_004152639.1 (uncharacterized protein LOC101222914 isoform X2 [Cucumis sativus] >KGN62693.1 hypothetical protein Csa_022618 [Cucumis sativus])

HSP 1 Score: 637.9 bits (1644), Expect = 4.5e-179
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0

Query: 1   MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 60
           MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI
Sbjct: 1   MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 60

Query: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 120
           GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF
Sbjct: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 120

Query: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 180
           SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 180

Query: 181 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240
           KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 241 IQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFL 300
           IQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFL
Sbjct: 241 IQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFL 300

Query: 301 VDP 304
           VDP
Sbjct: 301 VDP 303

BLAST of CSPI02G21030 vs. NCBI nr
Match: XP_031737138.1 (uncharacterized protein LOC101222914 isoform X1 [Cucumis sativus])

HSP 1 Score: 633.3 bits (1632), Expect = 1.1e-177
Identity = 303/304 (99.67%), Postives = 303/304 (99.67%), Query Frame = 0

Query: 1   MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 60
           MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI
Sbjct: 1   MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 60

Query: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 120
           GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF
Sbjct: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 120

Query: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 180
           SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 180

Query: 181 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240
           KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 241 IQQRTMIMWGEHDQVFPLELGHRLK-RHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF 300
           IQQRTMIMWGEHDQVFPLELGHRLK RHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF
Sbjct: 241 IQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF 300

Query: 301 LVDP 304
           LVDP
Sbjct: 301 LVDP 304

BLAST of CSPI02G21030 vs. NCBI nr
Match: KAA0065149.1 (2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase [Cucumis melo var. makuwa])

HSP 1 Score: 620.9 bits (1600), Expect = 5.7e-174
Identity = 293/303 (96.70%), Postives = 299/303 (98.68%), Query Frame = 0

Query: 1   MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 60
           MTKC FFSI ETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPI TKPNLLLIHGI
Sbjct: 1   MTKCLFFSITETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIRTKPNLLLIHGI 60

Query: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 120
           GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEA+TVGKF
Sbjct: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEAHTVGKF 120

Query: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 180
           SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVS LE+ATAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSDLEEATAILVPQ 180

Query: 181 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240
           KPEKLKQLVGYSFFRPPPLRL+PSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPLRLLPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 241 IQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFL 300
           IQQRT+IMWGEHDQVFP+ELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFL+HLISFL
Sbjct: 241 IQQRTLIMWGEHDQVFPVELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLNHLISFL 300

Query: 301 VDP 304
           VDP
Sbjct: 301 VDP 303

BLAST of CSPI02G21030 vs. NCBI nr
Match: XP_008444836.2 (PREDICTED: LOW QUALITY PROTEIN: 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase [Cucumis melo])

HSP 1 Score: 618.2 bits (1593), Expect = 3.7e-173
Identity = 292/303 (96.37%), Postives = 298/303 (98.35%), Query Frame = 0

Query: 1   MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 60
           MTKC FFSI ETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPI TKPNLLLIHGI
Sbjct: 1   MTKCLFFSITETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIRTKPNLLLIHGI 60

Query: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 120
           GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEA+TVGKF
Sbjct: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEAHTVGKF 120

Query: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 180
           SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVS LE+ATAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSDLEEATAILVPQ 180

Query: 181 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240
           KPEKLKQLVGYSF RPPPLRL+PSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFXRPPPLRLLPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 241 IQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFL 300
           IQQRT+IMWGEHDQVFP+ELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFL+HLISFL
Sbjct: 241 IQQRTLIMWGEHDQVFPVELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLNHLISFL 300

Query: 301 VDP 304
           VDP
Sbjct: 301 VDP 303

BLAST of CSPI02G21030 vs. NCBI nr
Match: XP_038885546.1 (uncharacterized protein LOC120075885 [Benincasa hispida])

HSP 1 Score: 597.4 bits (1539), Expect = 6.8e-167
Identity = 279/302 (92.38%), Postives = 292/302 (96.69%), Query Frame = 0

Query: 1   MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 60
           MT+CFFFSI ETKNW HRYSF KSGLRST+TDLKDGTTVHCWVP NPIHTKPNLLLIHGI
Sbjct: 1   MTRCFFFSITETKNWCHRYSFIKSGLRSTVTDLKDGTTVHCWVPNNPIHTKPNLLLIHGI 60

Query: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 120
           GANALWQWGDFIPAL+P+FNLYIPDLIFFGDSFTT+PDRTEWFQAQCLIRVMEAN+V K 
Sbjct: 61  GANALWQWGDFIPALVPHFNLYIPDLIFFGDSFTTRPDRTEWFQAQCLIRVMEANSVVKL 120

Query: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 180
           SLVGLSYGGFVGYSIAALRPEMVE+VVICCSG+CVEEKDFKDGLLKVS LE+ATAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVEKVVICCSGICVEEKDFKDGLLKVSDLEEATAILVPQ 180

Query: 181 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240
           KP+KLKQLVGYSFFRPPPLRL+PSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK
Sbjct: 181 KPDKLKQLVGYSFFRPPPLRLMPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 241 IQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFL 300
           I QRTMIMWGEHDQVFPLELGHRLKRHLG NATLVV+KN GHAFNSEEPKEFL+HLISFL
Sbjct: 241 IHQRTMIMWGEHDQVFPLELGHRLKRHLGANATLVVVKNAGHAFNSEEPKEFLNHLISFL 300

Query: 301 VD 303
           VD
Sbjct: 301 VD 302

BLAST of CSPI02G21030 vs. TAIR 10
Match: AT5G09430.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 379.4 bits (973), Expect = 2.7e-105
Identity = 171/301 (56.81%), Postives = 237/301 (78.74%), Query Frame = 0

Query: 7   FSIVETKNWFHRYSFTKSGLRSTITDLKDG-----TTVHCWVPKNPIHTKPNLLLIHGIG 66
           FS   +++W  R SF  +GLRS  TDL  G     T +HCW+PK+P  +KPNLLL+HG G
Sbjct: 11  FSFTASRDWLFRQSFANAGLRSVTTDLSHGNSIASTAMHCWIPKSPNRSKPNLLLLHGFG 70

Query: 67  ANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKFS 126
           ANA+WQ+G+ + A    FN+Y+PDL+FFG S T++P+RTE FQA+CL+R+MEA+ V + +
Sbjct: 71  ANAMWQYGEHLRAFTGRFNVYVPDLLFFGLSSTSEPNRTESFQARCLMRLMEAHGVQRMN 130

Query: 127 LVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQK 186
           +VG+SYGGFVGYS+AA  PE VE++V+CC+GVC+EEKD +DGL KV  LE+AT IL+PQ 
Sbjct: 131 IVGISYGGFVGYSLAAQFPENVEKLVLCCAGVCLEEKDMEDGLFKVPNLEEATGILIPQT 190

Query: 187 PEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPKI 246
           PEKLK+L+ +SF +  P++ +PS  L DFI+ MC + +EEKR+LI++I + R+LSDLP+I
Sbjct: 191 PEKLKELIRFSFVK--PIKGVPSFFLWDFIDVMCTEFVEEKRDLIKSILKDRRLSDLPRI 250

Query: 247 QQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFLV 303
           +Q+++I+WGE DQ+FPLELG+RLKRH+G++A +VVIK  GHA N E+ KEF+ HL SFL+
Sbjct: 251 KQKSLIIWGEEDQIFPLELGYRLKRHIGESAEIVVIKKAGHAVNLEKSKEFVKHLKSFLI 309

BLAST of CSPI02G21030 vs. TAIR 10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 364.8 bits (935), Expect = 6.9e-101
Identity = 174/305 (57.05%), Postives = 233/305 (76.39%), Query Frame = 0

Query: 1   MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHT--KPNLLLIH 60
           MT C  FS+ E     ++  F +SGLR    DLKDGT V+ WV K    +  KPNLLLIH
Sbjct: 1   MTGC--FSLSEALERTYKSGFKRSGLRPVTIDLKDGTVVNFWVSKTKPESKPKPNLLLIH 60

Query: 61  GIGANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVG 120
           G+GA A+WQW D    L  YFNLYIPDL+FFG S TT+P+R++ FQAQ L+R +EA +V 
Sbjct: 61  GLGATAIWQWYDVARRLSRYFNLYIPDLVFFGGSSTTRPERSDIFQAQTLMRALEAQSVK 120

Query: 121 KFSLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILV 180
           KFSLVGLSYGGFVGY +A++  + VE+VVICC+ VCVEEKD K G+ KVS L++A+ ILV
Sbjct: 121 KFSLVGLSYGGFVGYRMASMYADAVEKVVICCAAVCVEEKDMKAGVFKVSDLDEASKILV 180

Query: 181 PQKPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIE-SMCLDHIEEKRELIRTIPRGRKLSD 240
           P+  +KL++L+GY F++P   RL+P+CLL+DFIE ++  D++EEKRELI+ IP+ R +S+
Sbjct: 181 PESVKKLRELMGYIFYKPALARLVPTCLLHDFIEHALTRDNMEEKRELIKAIPKDRIISE 240

Query: 241 LPKIQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLI 300
           +PK++Q T+I+WGEHDQVFPLE+G RL++H+GDN  LV+IK TGH FN E+PK+F+  L 
Sbjct: 241 IPKLKQPTLIIWGEHDQVFPLEMGKRLEKHVGDNGKLVIIKRTGHIFNFEKPKKFIKLLK 300

Query: 301 SFLVD 303
           SFL++
Sbjct: 301 SFLLE 303

BLAST of CSPI02G21030 vs. TAIR 10
Match: AT4G39955.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 360.1 bits (923), Expect = 1.7e-99
Identity = 165/301 (54.82%), Postives = 224/301 (74.42%), Query Frame = 0

Query: 1   MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 60
           M  C  FS V ++N  ++YSF+++GLRS+ +DL DGT  HCW+P   IHTKP LLL+HGI
Sbjct: 1   MASC--FSYVSSRNKCYQYSFSRAGLRSSTSDLGDGTVFHCWIPLTHIHTKPTLLLLHGI 60

Query: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 120
           GANA+WQW  FI   IP FN+Y+PDLIFFGDS+TT+PDR+E FQA C+++ M+A  V   
Sbjct: 61  GANAMWQWDRFIDRFIPRFNVYVPDLIFFGDSYTTRPDRSESFQATCVMKAMDAYGVRTM 120

Query: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 180
           ++ GLSYGGFV YS+AA   E V+RVV+ C+GV +EEKD +DG+ KV + E+A A+L PQ
Sbjct: 121 TVAGLSYGGFVAYSLAAQFKERVDRVVLICAGVALEEKDSEDGMFKVKSPEEAAAVLFPQ 180

Query: 181 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240
            P  L++L+  SF++PP    IPSC   D+I  MC D+++E++EL+  + +GR+ ++LPK
Sbjct: 181 SPSMLRRLLQLSFYKPP--IWIPSCFAMDYIHVMCKDYLQERKELVEALHKGRRFANLPK 240

Query: 241 IQQRTMIMWGEHDQVFPLELGHRLKRHLG-DNATLVVIKNTGHAFNSEEPKEFLSHLISF 300
           I Q T+++WGE DQVFP+EL HRLKR+LG D A LV++K TGHA N E+PKE   H+ SF
Sbjct: 241 ITQPTLMIWGEEDQVFPVELAHRLKRYLGEDRAQLVLLKKTGHAINEEKPKEMYKHMKSF 297

BLAST of CSPI02G21030 vs. TAIR 10
Match: AT5G21950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 209.9 bits (533), Expect = 2.8e-54
Identity = 113/303 (37.29%), Postives = 170/303 (56.11%), Query Frame = 0

Query: 6   FFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVP----KNPIHTKPNLLLIHGIG 65
           F S+        R  F+ +GL      +   TT+  W P     +    KP+LLL+HG G
Sbjct: 5   FLSVARFVEALLRRRFSSAGLSLQTLSIDSETTIQFWGPPPSSSSENTQKPSLLLLHGFG 64

Query: 66  ANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKFS 125
            +A+WQW   +  L  +F LY+PDL+FFG S ++  +R+E FQA C+ ++ME   V +FS
Sbjct: 65  PSAVWQWSHQVKPLSHFFRLYVPDLVFFGGSSSSGENRSEMFQALCMGKLMEKLEVERFS 124

Query: 126 LVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQK 185
           +VG SYGGFV Y++A + PE VE+VV+  SGV +   D  +  +  +       +++P  
Sbjct: 125 VVGTSYGGFVAYNMAKMFPEKVEKVVLASSGVNLRRSD-NEAFIARAKCHRIKEVMLPAS 184

Query: 186 PEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGR-KLSDLPK 245
              L++  G        L  +P  +LNDF + M  +  EEK EL+  +  G+   +++  
Sbjct: 185 ATDLRRFSG--MVSSKRLDYVPDFVLNDFCQKMYSEKREEKAELLEGLSIGKDDKTNVSP 244

Query: 246 IQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFL 304
           IQQ  M++WGE DQVFPL++ H LK  LG  ATL VI+ T H   +E+ KEF   ++SFL
Sbjct: 245 IQQDVMLIWGEQDQVFPLKMAHDLKEMLGTKATLKVIQKTSHIPQTEKSKEFNGFVMSFL 304

BLAST of CSPI02G21030 vs. TAIR 10
Match: AT4G33180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 189.9 bits (481), Expect = 3.0e-48
Identity = 102/284 (35.92%), Postives = 166/284 (58.45%), Query Frame = 0

Query: 24  SGLRSTITDLKDGTTVHCWVPKNPIH----TKPNLLLIHGIGANALWQWGDFIPALIP-Y 83
           +GL S    +   TT+H W P    H     +P +LL+HG G +++WQW   + A  P  
Sbjct: 24  AGLTSQTLSIDSETTIHFWGPPPLDHRSDDDRPVMLLLHGFGPSSMWQWRRQMQAFSPSA 83

Query: 84  FNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKFSLVGLSYGGFVGYSIAAL 143
           F +Y PDL+FFGDS ++  +RTE FQA+C+ ++M    +GK+++ G SYGGFV Y +A +
Sbjct: 84  FRVYSPDLVFFGDSTSSSTNRTEVFQAECMAKLMAKIGIGKYNVAGTSYGGFVAYHMAKM 143

Query: 144 RPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQKPEKLKQLVGY-SFFRPP 203
            PE VE+VVI  SG+ + + D  + LL+ S  E    +++P    + + L+   S +R  
Sbjct: 144 WPEKVEKVVIASSGINMRKCD-GESLLQRSNCECIEKVMLPSTATEFRTLMALASSWR-- 203

Query: 204 PLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLS-DLPKIQQRTMIMWGEHDQVF 263
            +R+ P  L ND I ++   + +EK EL++ +  GR  + ++  + Q  +I+WG+ DQ+F
Sbjct: 204 LVRMFPDALWNDVINNLYKKNRKEKIELLKGVTFGRSENLNIDSLSQEVLIVWGDKDQIF 263

Query: 264 PLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFL 301
           P+++ + LK  LGD   L +I NT H    E  +EF + ++ FL
Sbjct: 264 PVKMAYELKEILGDKTKLEIIDNTSHVPQIECAQEFNNIVLRFL 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O052352.6e-1225.27Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yu... [more]
P911431.3e-0831.30Abhydrolase domain-containing protein abhd-5.1 OS=Caenorhabditis elegans OX=6239... [more]
Q5F6412.3e-0825.71Pimeloyl-[acyl-carrier protein] methyl ester esterase OS=Neisseria gonorrhoeae (... [more]
P9WNH22.3e-0821.67Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / ... [more]
P9WNH32.3e-0821.67Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 ... [more]
Match NameE-valueIdentityDescription
A0A0A0LLN72.2e-179100.00AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G368... [more]
A0A5A7VI512.8e-17496.702-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2, 4-dienoic acid hydrolase OS=Cucumis me... [more]
A0A1S3BC201.8e-17396.37LOW QUALITY PROTEIN: 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hyd... [more]
A0A6J1HDR21.1e-15986.38uncharacterized protein LOC111462585 OS=Cucurbita moschata OX=3662 GN=LOC1114625... [more]
A0A6J1KAV51.6e-15886.05uncharacterized protein LOC111492191 OS=Cucurbita maxima OX=3661 GN=LOC111492191... [more]
Match NameE-valueIdentityDescription
XP_004152639.14.5e-179100.00uncharacterized protein LOC101222914 isoform X2 [Cucumis sativus] >KGN62693.1 hy... [more]
XP_031737138.11.1e-17799.67uncharacterized protein LOC101222914 isoform X1 [Cucumis sativus][more]
KAA0065149.15.7e-17496.702-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase [Cucumis melo ... [more]
XP_008444836.23.7e-17396.37PREDICTED: LOW QUALITY PROTEIN: 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dieno... [more]
XP_038885546.16.8e-16792.38uncharacterized protein LOC120075885 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G09430.12.7e-10556.81alpha/beta-Hydrolases superfamily protein [more]
AT1G78210.16.9e-10157.05alpha/beta-Hydrolases superfamily protein [more]
AT4G39955.11.7e-9954.82alpha/beta-Hydrolases superfamily protein [more]
AT5G21950.12.8e-5437.29alpha/beta-Hydrolases superfamily protein [more]
AT4G33180.13.0e-4835.92alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 52..151
e-value: 2.4E-10
score: 40.5
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 16..217
e-value: 7.0E-26
score: 93.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 25..196
NoneNo IPR availablePANTHERPTHR43139SI:DKEY-122A22.2coord: 6..213
NoneNo IPR availablePANTHERPTHR43139:SF54ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 6..213

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G21030.1CSPI02G21030.1mRNA
CSPI02G21030.2CSPI02G21030.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016787 hydrolase activity