CSPI02G20470 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G20470
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Cysteine protease
Location	Chr2: 18133292 .. 18134745 (-)
RNA-Seq Expression	CSPI02G20470
Synteny	CSPI02G20470

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAAAGATTGCTTGCTGACTTGCATTTTATGTTTATTGAGCTTGCAGCTGAAGAGGAATGTTGTTACCATTGATGGTTATACTTATATTCCTTCAAATGATGAGGGAAAATTACTGCAAGCAGTAGCAACTCAACCTGTGAGTGTTAGCATATGTGGCAGTGATAGAGCCTTTCAATTATACTCAAAGGTTGGTCCTACTCGAAACTGCTAATTGAAAGAGGTTCCATCATATAAGAAATTATAACTAAAAATCATGAGTTGTATTTACTTGCTTGGTAGGGAATTTTCTCCGGTCCGTGTTCAACTTCTTTGGATCATGGTGTGTTGATTGTAGGATATGGCTCAGAAATGGAGTTGATTATTGGATTGTGAAGAACTCATGGGGTAGAAGTTGGGGAATGGATGGTTACATCCACATGCAGCGCAACAGCGGCAATGCGGAAGGCCTTTGTGGAATTAACAAGCTTGCTTCATATCCAACTACAACAAATCCTAACCCTCCTCCAGGTCCAACAAAATGTAGTGCTATTTGTTGGTGATATATAATTAAATTTGCCTTCAACCACTAGCTTAAACTTTTGGATAAATTGGTGGTTCAATATGTTGGAATTCTTTCCTTTTTTTCTAGGCTTTTTCCTTGTTAGAATCCTAGAATAATTATGGAAAGATTATGGGAATGTATTCCTTATTTTCCTAAATCTTTTCCTTTTTTATTCCATTGTGTACTCTATTTATTCTCCCTTGTACCTATTGCTTTATTCATTAGAAAATAATAACAACACAAACAATCGTGGTTTTTCTCCCGGTACTCGGGTTTCCACGTAAATTGATGTGAACTCGTTGTCTCTCTTTTCAATATATTTATTATGGGTTTGATCTCAAAGGCTTCTTTTCGGTCAAGAGTGCTTACCATTTGGCTATTAGTCTCAACGAGCCTAAAGAGGCTTTGGTTTTGGATGAGTTTAATATGAAAAAGTGTTGGAACGCTTATGGAAGACTAAGGTCTTTCCTAAAGTGAAGATTTGTATGTGGCAACTGTTAAATGATGTTATCCCTTCAAAGGGCTCAACTTCGAAAGAATGGAATCGAGATAGGCACTTTTTGAATTTTCTGTATAATTGAGAAGGAGTCTACCATCCATCTCTTTTTCACATTGCTGGTTTGTTAAACAAACATGGTCCTATTTTTCTTCCTTATTTATGTTTGCCTCTTTTCTTGTGTAGGGACAGTTTAATAAGTTAAACTTATGAGATTGGTTAGTTGTAAAGCTATTTAGGAGTTTGGTACCACTATTTTGATTTAGTCGTGTAGAAACTAATGGAATGCCACGAGTTCTATCCCTCATGTATTGAAAGTTGTGTTGTGCAGGCATCTCGTCTGATTGGTCGTGTCACTTTTGAGGTCACTTGTACAGGGCAGTTTGATATTACTCTTTTAGTGAAGAGTTAG

mRNA sequence

ATGGGAAAAGATTGCTTGCTGACTTGCATTTTATGTTTATTGAGCTTGCAGCTGAAGAGGAATGTTGTTACCATTGATGGTTATACTTATATTCCTTCAAATGATGAGGGAAAATTACTGCAAGCAGTAGCAACTCAACCTGTGAGTGTTAGCATATGTGGCAGTGATAGAGCCTTTCAATTATACTCAAAGGTTGGGAATTTTCTCCGGTCCGTGTTCAACTTCTTTGGATCATGGTGTGTTGATTGTAGGATATGGCTCAGAAATGGAGTTGATTATTGGATTGTGAAGAACTCATGGGGTAGAAGTTGGGGAATGGATGGTTACATCCACATGCAGCGCAACAGCGGCAATGCGGAAGGCCTTTGTGGAATTAACAAGCTTGCTTCATATCCAACTACAACAAATCCTAACCCTCCTCCAGGCTTCTTTTCGGTCAAGAGTGCTTACCATTTGGCTATTAGTCTCAACGAGCCTAAAGAGGCTTTGGCATCTCGTCTGATTGGTCGTGTCACTTTTGAGGTCACTTGTACAGGGCAGTTTGATATTACTCTTTTAGTGAAGAGTTAG

Coding sequence (CDS)

ATGGGAAAAGATTGCTTGCTGACTTGCATTTTATGTTTATTGAGCTTGCAGCTGAAGAGGAATGTTGTTACCATTGATGGTTATACTTATATTCCTTCAAATGATGAGGGAAAATTACTGCAAGCAGTAGCAACTCAACCTGTGAGTGTTAGCATATGTGGCAGTGATAGAGCCTTTCAATTATACTCAAAGGTTGGGAATTTTCTCCGGTCCGTGTTCAACTTCTTTGGATCATGGTGTGTTGATTGTAGGATATGGCTCAGAAATGGAGTTGATTATTGGATTGTGAAGAACTCATGGGGTAGAAGTTGGGGAATGGATGGTTACATCCACATGCAGCGCAACAGCGGCAATGCGGAAGGCCTTTGTGGAATTAACAAGCTTGCTTCATATCCAACTACAACAAATCCTAACCCTCCTCCAGGCTTCTTTTCGGTCAAGAGTGCTTACCATTTGGCTATTAGTCTCAACGAGCCTAAAGAGGCTTTGGCATCTCGTCTGATTGGTCGTGTCACTTTTGAGGTCACTTGTACAGGGCAGTTTGATATTACTCTTTTAGTGAAGAGTTAG

Protein sequence

MGKDCLLTCILCLLSLQLKRNVVTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFNFFGSWCVDCRIWLRNGVDYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLASYPTTTNPNPPPGFFSVKSAYHLAISLNEPKEALASRLIGRVTFEVTCTGQFDITLLVKS*

Homology

[Close]

BLAST of CSPI02G20470 vs. ExPASy Swiss-Prot
Match: P25251 (Cysteine proteinase COT44 (Fragment) OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 1.9e-24
Identity = 65/132 (49.24%), Postives = 73/132 (55.30%), Query Frame = 0


Query: 15  SLQLKRNVVTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFN 74
           SL     VVTIDGY  +PS DE  L +AV+ QPVSV+I    RAFQ Y   G F      
Sbjct: 198 SLLKNSRVVTIDGYEDVPSKDETALKRAVSYQPVSVAIDAGGRAFQHYQS-GIFTGKCGT 257

Query: 75  FFGSWCVDCRIWLRNGVDYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLASYPTT 134
                 V       NGVDYWIV+NSWG  WG DGYI M+RN  +  G CGI   ASYP  
Sbjct: 258 NMDHAVVAVGYGSENGVDYWIVRNSWGTRWGEDGYIRMERNVASKSGKCGIAIEASYPVK 317

Query: 135 TNPNPPPGFFSV 147
            +PNP  G  SV
Sbjct: 318 YSPNPVRGTSSV 328

[Close]

BLAST of CSPI02G20470 vs. ExPASy Swiss-Prot
Match: P25777 (Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1 SV=2)

HSP 1 Score: 107.5 bits (267), Expect = 1.7e-22
Identity = 57/119 (47.90%), Postives = 67/119 (56.30%), Query Frame = 0


Query: 22  VVTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFNFFGSWCV 81
           VV+IDG+  +P NDE  L +AVA QPVSV+I    R FQLY   G F            V
Sbjct: 247 VVSIDGFEDVPQNDEKSLQKAVAHQPVSVAIEAGGREFQLYHS-GVFSGRCGTSLDHGVV 306

Query: 82  DCRIWLRNGVDYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLASYPTTTNPNPP 141
                  NG DYWIV+NSWG  WG  GY+ M+RN     G CGI  +ASYPT +  NPP
Sbjct: 307 AVGYGTDNGKDYWIVRNSWGPKWGESGYVRMERNINVTTGKCGIAMMASYPTKSGANPP 364

[Close]

BLAST of CSPI02G20470 vs. ExPASy Swiss-Prot
Match: Q9LT78 (Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.7e-22
Identity = 54/120 (45.00%), Postives = 71/120 (59.17%), Query Frame = 0


Query: 22  VVTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFNFFGSWCV 81
           VVTIDGY  +P NDE  L +A+A QP+SV+I    RAFQLY+  G F  +         V
Sbjct: 235 VVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQLYTS-GVFTGTCGTSLDHGVV 294

Query: 82  DCRIWLRNGVDYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLASYPTTTNPNPPP 141
                   G DYWIV+NSWG +WG  GY  ++RN   + G CG+  +ASYPT ++ + PP
Sbjct: 295 AVGYGSEGGQDYWIVRNSWGSNWGESGYFKLERNIKESSGKCGVAMMASYPTKSSGSNPP 353

[Close]

BLAST of CSPI02G20470 vs. ExPASy Swiss-Prot
Match: P25803 (Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 107.1 bits (266), Expect = 2.3e-22
Identity = 60/135 (44.44%), Postives = 73/135 (54.07%), Query Frame = 0


Query: 23  VTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFNFFGSWCVD 82
           V+IDG+  +P+NDE  LL+AVA QPVSV+I      FQ YS+            G +  D
Sbjct: 234 VSIDGHENVPANDEDALLKAVANQPVSVAIDAGGSDFQFYSE------------GVFTGD 293

Query: 83  CRIWLRNGV------------DYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLAS 142
           C   L +GV            +YWIV+NSWG  WG  GYI MQRN    EGLCGI  L S
Sbjct: 294 CSTDLNHGVAIVGYGTTVDGTNYWIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPS 353

Query: 143 YPTTTNPNPPPGFFS 146
           YP   + + P G FS
Sbjct: 354 YPIKNSSDNPTGSFS 356

[Close]

BLAST of CSPI02G20470 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 3.0e-22
Identity = 63/153 (41.18%), Postives = 78/153 (50.98%), Query Frame = 0


Query: 23  VTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFNFFGSWCVD 82
           V+IDG+  +P NDE  LL+AVA QPVSV+I      FQ YS+            G +  D
Sbjct: 234 VSIDGHENVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSE------------GVFTGD 293

Query: 83  CRIWLRNGV------------DYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLAS 142
           C   L +GV            +YWIV+NSWG  WG  GYI MQRN    EGLCGI  +AS
Sbjct: 294 CNTDLNHGVAIVGYGTTVDGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMAS 353

Query: 143 YPTTTNPNPPPGFFSVKSAYHLAISLNEPKEAL 164
           YP   + + P G            SL+ PK+ L
Sbjct: 354 YPIKNSSDNPTG------------SLSSPKDEL 362

[Close]

BLAST of CSPI02G20470 vs. ExPASy TrEMBL
Match: A0A0A0LNP7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G362510 PE=3 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 6.4e-44
Identity = 92/130 (70.77%), Postives = 104/130 (80.00%), Query Frame = 0


Query: 17  QLKRNVVTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFNFF 76
           +L+RNVVTIDGY  IPSNDEGKLLQAVA QPVSV ICGS+RAFQLYSK       +F+  
Sbjct: 214 KLQRNVVTIDGYADIPSNDEGKLLQAVAAQPVSVGICGSERAFQLYSK------GIFSGP 273

Query: 77  GSWCVDCRIWL-----RNGVDYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLASY 136
            S  +D  + +      NGVDYWIVKNSWG+SWGMDGY+HMQRNSGN+EG+CGINKLASY
Sbjct: 274 CSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMDGYMHMQRNSGNSEGVCGINKLASY 333

Query: 137 PTTTNPNPPP 142
           PT TNPNPPP
Sbjct: 334 PTKTNPNPPP 337

[Close]

BLAST of CSPI02G20470 vs. ExPASy TrEMBL
Match: A0A5A7VC45 (Zingipain-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005350 PE=3 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 2.4e-43
Identity = 91/130 (70.00%), Postives = 104/130 (80.00%), Query Frame = 0


Query: 17  QLKRNVVTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFNFF 76
           +L+RNVVTIDGYT IP NDEGKLLQAVA QPVSV ICGS+RAFQLYSK       +F+  
Sbjct: 213 KLQRNVVTIDGYTDIPPNDEGKLLQAVAAQPVSVGICGSERAFQLYSK------GIFSGP 272

Query: 77  GSWCVDCRIWL-----RNGVDYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLASY 136
            S  +D  + +      NGVDYWIVKNSWG+SWGMDGY+HMQRNSGN+EG+CGINKLASY
Sbjct: 273 CSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMDGYMHMQRNSGNSEGVCGINKLASY 332

Query: 137 PTTTNPNPPP 142
           PT T+PNPPP
Sbjct: 333 PTKTSPNPPP 336

[Close]

BLAST of CSPI02G20470 vs. ExPASy TrEMBL
Match: A0A1S3BBY8 (zingipain-2 OS=Cucumis melo OX=3656 GN=LOC103488009 PE=3 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 2.4e-43
Identity = 91/130 (70.00%), Postives = 104/130 (80.00%), Query Frame = 0


Query: 17  QLKRNVVTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFNFF 76
           +L+RNVVTIDGYT IP NDEGKLLQAVA QPVSV ICGS+RAFQLYSK       +F+  
Sbjct: 214 KLQRNVVTIDGYTDIPPNDEGKLLQAVAAQPVSVGICGSERAFQLYSK------GIFSGP 273

Query: 77  GSWCVDCRIWL-----RNGVDYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLASY 136
            S  +D  + +      NGVDYWIVKNSWG+SWGMDGY+HMQRNSGN+EG+CGINKLASY
Sbjct: 274 CSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMDGYMHMQRNSGNSEGVCGINKLASY 333

Query: 137 PTTTNPNPPP 142
           PT T+PNPPP
Sbjct: 334 PTKTSPNPPP 337

[Close]

BLAST of CSPI02G20470 vs. ExPASy TrEMBL
Match: A0A6J1GH92 (zingipain-2 OS=Cucurbita moschata OX=3662 GN=LOC111454188 PE=3 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 2.1e-39
Identity = 85/130 (65.38%), Postives = 99/130 (76.15%), Query Frame = 0


Query: 17  QLKRNVVTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFNFF 76
           +L R VVTIDGY+ +P N+E KLLQAVA QPVSV ICGS+RAFQLYSK       +F+  
Sbjct: 214 KLNRKVVTIDGYSDVPPNNEEKLLQAVAIQPVSVGICGSERAFQLYSK------GIFSGP 273

Query: 77  GSWCVDCRIWL-----RNGVDYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLASY 136
            S  +D  + +      NGVDYWIVKNSWG+ WGMDGYIHMQRNSGN+EG+CGIN LASY
Sbjct: 274 CSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRWGMDGYIHMQRNSGNSEGVCGINMLASY 333

Query: 137 PTTTNPNPPP 142
           PT T+PNPPP
Sbjct: 334 PTKTSPNPPP 337

[Close]

BLAST of CSPI02G20470 vs. ExPASy TrEMBL
Match: A0A6P6AW24 (low-temperature-induced cysteine proteinase OS=Durio zibethinus OX=66656 GN=LOC111312764 PE=3 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 4.7e-39
Identity = 84/125 (67.20%), Postives = 95/125 (76.00%), Query Frame = 0


Query: 17  QLKRNVVTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFNFF 76
           +LKR VVTIDGYT IP+N+E +LLQAVATQPVSV ICGS+RAFQLYSK G F     N  
Sbjct: 218 KLKRRVVTIDGYTDIPANNEKQLLQAVATQPVSVGICGSERAFQLYSK-GIFTGPCSNSL 277

Query: 77  GSWCVDCRIWLRNGVDYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLASYPTTTN 136
               +       NG+DYWIVKNSWG  WGM+GYIHM RN GN+EG+CGIN LASYPT T+
Sbjct: 278 DHAVLIVGYGSENGMDYWIVKNSWGTRWGMNGYIHMIRNGGNSEGICGINMLASYPTKTS 337

Query: 137 PNPPP 142
           PNPPP
Sbjct: 338 PNPPP 341

[Close]

BLAST of CSPI02G20470 vs. NCBI nr
Match: XP_004152671.1 (zingipain-2 [Cucumis sativus] >KGN62639.1 hypothetical protein Csa_022234 [Cucumis sativus])

HSP 1 Score: 187.2 bits (474), Expect = 1.3e-43
Identity = 92/130 (70.77%), Postives = 104/130 (80.00%), Query Frame = 0


Query: 17  QLKRNVVTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFNFF 76
           +L+RNVVTIDGY  IPSNDEGKLLQAVA QPVSV ICGS+RAFQLYSK       +F+  
Sbjct: 214 KLQRNVVTIDGYADIPSNDEGKLLQAVAAQPVSVGICGSERAFQLYSK------GIFSGP 273

Query: 77  GSWCVDCRIWL-----RNGVDYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLASY 136
            S  +D  + +      NGVDYWIVKNSWG+SWGMDGY+HMQRNSGN+EG+CGINKLASY
Sbjct: 274 CSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMDGYMHMQRNSGNSEGVCGINKLASY 333

Query: 137 PTTTNPNPPP 142
           PT TNPNPPP
Sbjct: 334 PTKTNPNPPP 337

[Close]

BLAST of CSPI02G20470 vs. NCBI nr
Match: KAA0065198.1 (zingipain-2 [Cucumis melo var. makuwa])

HSP 1 Score: 185.3 bits (469), Expect = 5.0e-43
Identity = 91/130 (70.00%), Postives = 104/130 (80.00%), Query Frame = 0


Query: 17  QLKRNVVTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFNFF 76
           +L+RNVVTIDGYT IP NDEGKLLQAVA QPVSV ICGS+RAFQLYSK       +F+  
Sbjct: 213 KLQRNVVTIDGYTDIPPNDEGKLLQAVAAQPVSVGICGSERAFQLYSK------GIFSGP 272

Query: 77  GSWCVDCRIWL-----RNGVDYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLASY 136
            S  +D  + +      NGVDYWIVKNSWG+SWGMDGY+HMQRNSGN+EG+CGINKLASY
Sbjct: 273 CSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMDGYMHMQRNSGNSEGVCGINKLASY 332

Query: 137 PTTTNPNPPP 142
           PT T+PNPPP
Sbjct: 333 PTKTSPNPPP 336

[Close]

BLAST of CSPI02G20470 vs. NCBI nr
Match: XP_008444761.1 (PREDICTED: zingipain-2 [Cucumis melo])

HSP 1 Score: 185.3 bits (469), Expect = 5.0e-43
Identity = 91/130 (70.00%), Postives = 104/130 (80.00%), Query Frame = 0


Query: 17  QLKRNVVTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFNFF 76
           +L+RNVVTIDGYT IP NDEGKLLQAVA QPVSV ICGS+RAFQLYSK       +F+  
Sbjct: 214 KLQRNVVTIDGYTDIPPNDEGKLLQAVAAQPVSVGICGSERAFQLYSK------GIFSGP 273

Query: 77  GSWCVDCRIWL-----RNGVDYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLASY 136
            S  +D  + +      NGVDYWIVKNSWG+SWGMDGY+HMQRNSGN+EG+CGINKLASY
Sbjct: 274 CSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMDGYMHMQRNSGNSEGVCGINKLASY 333

Query: 137 PTTTNPNPPP 142
           PT T+PNPPP
Sbjct: 334 PTKTSPNPPP 337

[Close]

BLAST of CSPI02G20470 vs. NCBI nr
Match: XP_038885598.1 (zingipain-2 [Benincasa hispida])

HSP 1 Score: 174.9 bits (442), Expect = 6.8e-40
Identity = 87/130 (66.92%), Postives = 101/130 (77.69%), Query Frame = 0


Query: 17  QLKRNVVTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFNFF 76
           +LKRN VTIDGYT IP N+EGKLLQAVA QPVSV ICGS+RAFQLYSK       +F+  
Sbjct: 214 KLKRNAVTIDGYTDIPPNNEGKLLQAVAAQPVSVGICGSERAFQLYSK------GIFSGP 273

Query: 77  GSWCVDCRIWL-----RNGVDYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLASY 136
            S  +D  + +      NGVDYWIVKNSWG+SWGMDGY+ MQRNSG++EG+CGIN LASY
Sbjct: 274 CSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMDGYMLMQRNSGSSEGVCGINMLASY 333

Query: 137 PTTTNPNPPP 142
           PT T+PNPPP
Sbjct: 334 PTKTSPNPPP 337

[Close]

BLAST of CSPI02G20470 vs. NCBI nr
Match: XP_023538152.1 (zingipain-2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 172.2 bits (435), Expect = 4.4e-39
Identity = 85/130 (65.38%), Postives = 99/130 (76.15%), Query Frame = 0


Query: 17  QLKRNVVTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFNFF 76
           +L R VVTIDGY+ +P N+E KLLQAVA QPVSV ICGS+RAFQLYSK       +F+  
Sbjct: 214 KLNRKVVTIDGYSDVPPNNEEKLLQAVAIQPVSVGICGSERAFQLYSK------GIFSGP 273

Query: 77  GSWCVDCRIWL-----RNGVDYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLASY 136
            S  +D  + +      NGVDYWIVKNSWG+ WGMDGYIHMQRNSGN+EG+CGIN LASY
Sbjct: 274 CSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRWGMDGYIHMQRNSGNSEGVCGINMLASY 333

Query: 137 PTTTNPNPPP 142
           PT T+PNPPP
Sbjct: 334 PTKTSPNPPP 337

[Close]

BLAST of CSPI02G20470 vs. TAIR 10
Match: AT1G09850.1 (xylem bark cysteine peptidase 3 )

HSP 1 Score: 156.8 bits (395), Expect = 1.8e-38
Identity = 76/130 (58.46%), Postives = 96/130 (73.85%), Query Frame = 0


Query: 17  QLKRNVVTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFNFF 76
           +LK+ VVTID Y  + SNDE  L++AVA QPVSV ICGS+RAFQLYS        +F+  
Sbjct: 218 KLKQKVVTIDSYAGVKSNDEKALMEAVAAQPVSVGICGSERAFQLYSS------GIFSGP 277

Query: 77  GSWCVDCRIWL-----RNGVDYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLASY 136
            S  +D  + +     +NGVDYWIVKNSWG+SWGMDG++HMQRN+ N++G+CGIN LASY
Sbjct: 278 CSTSLDHAVLIVGYGSQNGVDYWIVKNSWGKSWGMDGFMHMQRNTENSDGVCGINMLASY 337

Query: 137 PTTTNPNPPP 142
           P  T+PNPPP
Sbjct: 338 PIKTHPNPPP 341

[Close]

BLAST of CSPI02G20470 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 107.5 bits (267), Expect = 1.2e-23
Identity = 54/120 (45.00%), Postives = 71/120 (59.17%), Query Frame = 0


Query: 22  VVTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFNFFGSWCV 81
           VVTIDGY  +P NDE  L +A+A QP+SV+I    RAFQLY+  G F  +         V
Sbjct: 235 VVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQLYTS-GVFTGTCGTSLDHGVV 294

Query: 82  DCRIWLRNGVDYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLASYPTTTNPNPPP 141
                   G DYWIV+NSWG +WG  GY  ++RN   + G CG+  +ASYPT ++ + PP
Sbjct: 295 AVGYGSEGGQDYWIVRNSWGSNWGESGYFKLERNIKESSGKCGVAMMASYPTKSSGSNPP 353

[Close]

BLAST of CSPI02G20470 vs. TAIR 10
Match: AT1G47128.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 105.1 bits (261), Expect = 6.1e-23
Identity = 59/129 (45.74%), Postives = 73/129 (56.59%), Query Frame = 0


Query: 17  QLKRN--VVTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFN 76
           Q+++N  VVTID Y  +P+  E  L +AVA QP+S++I    RAFQLY   G F  S   
Sbjct: 235 QIRKNAKVVTIDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDS-GIFDGSCGT 294

Query: 77  FFGSWCVDCRIWLRNGVDYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLASYPTT 136
                 V       NG DYWIV+NSWG+SWG  GY+ M RN  ++ G CGI    SYP  
Sbjct: 295 QLDHGVVAVGYGTENGKDYWIVRNSWGKSWGESGYLRMARNIASSSGKCGIAIEPSYPIK 354

Query: 137 TNPNPP-PG 143
              NPP PG
Sbjct: 355 NGENPPNPG 362

[Close]

BLAST of CSPI02G20470 vs. TAIR 10
Match: AT4G35350.1 (xylem cysteine peptidase 1 )

HSP 1 Score: 104.8 bits (260), Expect = 8.0e-23
Identity = 57/113 (50.44%), Postives = 67/113 (59.29%), Query Frame = 0


Query: 23  VTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFNFFGSWCVD 82
           VTI GY  +P ND+  L++A+A QPVSV+I  S R FQ Y K G F              
Sbjct: 243 VTISGYEDVPENDDESLVKALAHQPVSVAIEASGRDFQFY-KGGVFNGKCGTDLDHGVAA 302

Query: 83  CRIWLRNGVDYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLASYPTTT 136
                  G DY IVKNSWG  WG  G+I M+RN+G  EGLCGINK+ASYPT T
Sbjct: 303 VGYGSSKGSDYVIVKNSWGPRWGEKGFIRMKRNTGKPEGLCGINKMASYPTKT 354

[Close]

BLAST of CSPI02G20470 vs. TAIR 10
Match: AT1G20850.1 (xylem cysteine peptidase 2 )

HSP 1 Score: 104.4 bits (259), Expect = 1.0e-22
Identity = 58/124 (46.77%), Postives = 74/124 (59.68%), Query Frame = 0


Query: 23  VTIDGYTYIPSNDEGKLLQAVATQPVSVSICGSDRAFQLYSKVGNFLRSVFNFFGSWCVD 82
           VTI+G+  +P+NDE  LL+A+A QP+SV+I  S R FQ YS             G +   
Sbjct: 244 VTINGHQDVPTNDEKSLLKALAHQPLSVAIDASGREFQFYSG------------GVFDGR 303

Query: 83  CRIWLRNGV-----------DYWIVKNSWGRSWGMDGYIHMQRNSGNAEGLCGINKLASY 136
           C + L +GV           DY IVKNSWG  WG  GYI ++RN+G  EGLCGINK+AS+
Sbjct: 304 CGVDLDHGVAAVGYGSSKGSDYIIVKNSWGPKWGEKGYIRLKRNTGKPEGLCGINKMASF 355

The following BLAST results are available for this feature:

BLAST of CSPI02G20470 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P25251	1.9e-24	49.24	Cysteine proteinase COT44 (Fragment) OS=Brassica napus OX=3708 PE=2 SV=1	[more]
P25777	1.7e-22	47.90	Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1...	[more]
Q9LT78	1.7e-22	45.00	Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 S...	[more]
P25803	2.3e-22	44.44	Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2	[more]
P12412	3.0e-22	41.18	Vignain OS=Vigna mungo OX=3915 PE=1 SV=1	[more]

BLAST of CSPI02G20470 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LNP7	6.4e-44	70.77	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G362510 PE=3 SV=1	[more]
A0A5A7VC45	2.4e-43	70.00	Zingipain-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005350 PE...	[more]
A0A1S3BBY8	2.4e-43	70.00	zingipain-2 OS=Cucumis melo OX=3656 GN=LOC103488009 PE=3 SV=1	[more]
A0A6J1GH92	2.1e-39	65.38	zingipain-2 OS=Cucurbita moschata OX=3662 GN=LOC111454188 PE=3 SV=1	[more]
A0A6P6AW24	4.7e-39	67.20	low-temperature-induced cysteine proteinase OS=Durio zibethinus OX=66656 GN=LOC1...	[more]

BLAST of CSPI02G20470 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152671.1	1.3e-43	70.77	zingipain-2 [Cucumis sativus] >KGN62639.1 hypothetical protein Csa_022234 [Cucum...	[more]
KAA0065198.1	5.0e-43	70.00	zingipain-2 [Cucumis melo var. makuwa]	[more]
XP_008444761.1	5.0e-43	70.00	PREDICTED: zingipain-2 [Cucumis melo]	[more]
XP_038885598.1	6.8e-40	66.92	zingipain-2 [Benincasa hispida]	[more]
XP_023538152.1	4.4e-39	65.38	zingipain-2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI02G20470 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G09850.1	1.8e-38	58.46	xylem bark cysteine peptidase 3	[more]
AT3G19390.1	1.2e-23	45.00	Granulin repeat cysteine protease family protein	[more]
AT1G47128.1	6.1e-23	45.74	Granulin repeat cysteine protease family protein	[more]
AT4G35350.1	8.0e-23	50.44	xylem cysteine peptidase 1	[more]
AT1G20850.1	1.0e-22	46.77	xylem cysteine peptidase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 1..133 e-value: 7.3E-4 score: -43.7
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 17..132 e-value: 8.3E-20 score: 71.8
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 4..135 e-value: 2.4E-25 score: 91.7
None	No IPR available	PANTHER	PTHR12411:SF414	OS05G0508300 PROTEIN	coord: 16..155
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 16..155
IPR025661	Cysteine peptidase, asparagine active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 93..112
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 19..133

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G20470.1	CSPI02G20470.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008234	cysteine-type peptidase activity