CSPI02G20150 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G20150
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	isoflavone reductase-like protein
Location	Chr2: 17973035 .. 17975940 (-)
RNA-Seq Expression	CSPI02G20150
Synteny	CSPI02G20150

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGCATATAAATACCTTTGGTCTTAAGGGGAGGAAGACGATCCAAGCAATTTGCGTTTCTTCAGCGAAAGAAGATATTTGAAGTTATTGAAAATGACGGAGAATGGTCAGAAGAGCAAAGTTCTTATCATCGGCGGCACTGGATATATCGGAAAGTTTGTCGTGGAAGCGAGCGCTAAGGCCGCGCATCCTACGTTTGTTCTCGTCAGAGAGAGCACCATTGTCGATCCTGTAAAAGCAAAACTCTTGGAGAGTTTTAAGAATTTAGGCGTTAAATTAATCACTGTGAGTTACGTTTGATTTTAATGAATTGTGATTTGGTTGATTGATCTCTGCTTTTTCTATATTCTTCGAGAATAGTTGTGATTCGTGATTGAATTGAGGAATGTGAGTTGGTTTTTAATTGTGGAATAATGTAGGGGGATCTGTATGATCATGAAGGTTTAGTGAAAGCGATAAAGCAAGTGGATGTTGTGATCTCGACTGTTGGACAAATGCAGTTGGCAGATCAGAGCAAGATCGTTGATGCGATTAAAGAGGCCGGCAATGTCAAGGTAGCGAACTTAGTCACTCATTGATTCTCACGATTTGCTTTTATCCCTAATTCACAATATATCTGGTCGTGTTTGGCGAAATGAAATCATCTGCTCCACGGTTAGACACAGAGTTACAAATTTCTGAACTCTTTTTCAACTTTGATTCTTCCTGACAAATTATTTGCACTGAACTTTCGGGATTTTTTAATGGAAAAAACTAGTGTATAAATTGAAAGCAAAGATTAATATCACTTATGGAATAAATATTTGAAACACAACATTTAGGGACAAAACTTATGATTTACACGAATACAAATCTCTTAGTCGCTAAAATGTGCATACTATATGTCATTAACATTATTGATAACTGGATGCTGCAAATATAGCTATTATTTTTCATATCAATATATATTCAAGTTCACCGTCTTAAAAAGGTAAAAATTATGTTTAATTTTGATATTTAATAAAATGTTGATATTGATGGAAAAGATCTCAAGTTGGGAGAAATGTTGATGAAAAAATCGGTGAAATGAATATTTATAGAAAATAATAATAATAAACAAACAATTTACACAGGTAAATGTATTTATATTTGTTCCCATTATCACTGTCTTCTTCATTTTAAGTTATGGATCATTGATTCGGATATAATGAATATGTCGACTCAGTTCTTACGTTAATAATCAGATGACTACTACACATATGAGAATTTTCAAAAAACAAAAAACAAAAAAGAAACGTAGATATGAAATGCTAATATTTGAAATGAAAGAGAGGAAAAAAAGATGATAGTTGAGAAAGTGGTGTTGCTTAATTTGGAGTACGATGGATGCAGAGATTTTTCCCATCGGAGTTTGGAGTGGATGTGGATCGACTCCACGCAGTTGAGCCAGCAAAATCTGCACTGGCCGTAAAGGCTAATATCAGAAGGGCCATTGAGAAGGAAGGAATACCTTACACTTACGTCGTCTCCAACTGTTTTAATGGCTACTTCCTCCCCACTCTGATGCAGCCTGGACTCACCTCCCCTCCCACTCACAAGGTCGTCATTCCCGGCGACGGACACCCAAAAGGTCAGTCACCATTTACCAACCAAATTCAATTTCTATGTCATTAATTTTACTAATGATCTTTATACTCTTGATTTTGGTTCATTTTACTCGGTTTGTTTTAGTTCTTATAGTAAAAATGTTCACCGAAAATGGACCTTTCGATTATTTTCTTTAATTTTTATTTTGAAACACTCTTTTGTGTTTGTAAGTGATAATTTTGGTTGACAAATAAAACAAATACACAGCAATATTTAACTTGGAGGAAGACATCGGGACTTATACCATTAAAGCAGTGGATGATCCAAGAACTGAAAACAAAATATTGTATATCAAGCCTCCCAACAACACCTATTCTTTCAACGACCTAGTCGCTCTGTGGGAGAAGAAGATCGGCAAACCCTTGGAAAAGCTCTATGTTCCCGAACACCAAATCCTTAAGGACATTCAAGGTCAGTCTTTCTTAACTCACCGAAATATTAATCCATTCAATTCACTAAATATCTAAATGCTTACCACCATAGGGCTCTGTTCTTCTTTCTTTTGAACAATCTTCCCCTTGGACCATTTTTAAATTTATTAGCATAGTTATTATTATCATTACAAAATTATATATACCAATAAATTGATTAGTAATTATTTCGTTCTATCAGAAAATTTCTTTAAATTCAAACTGTCCAGGTGATATATGATTAATTATATTCTATAGAAAAAAAAAAGTAATCAACGATCATCACCTCACCAAACTATTGTAAGATTTCAGTCAAATTTCTAGCATAGATTCCAATGTTTTAACCGGATGTCTGTTTGAGTTCTATGTGATATAACAATAAGAACAGGTGAGGATTAATTTAGCTCTAAATTTCAACTTCTTAGAGACATACATATATATATCTAATACACAATGTGAATTAATTTGGCAGAGGCTCCACTTCCTTTGAATGTGATATTGGGTCTCAACCACTCAATATTTGTGAAAGGAGATGAGACCAATTTTGAAATTGAAGCATCTTTTGGTGTGGAGGCTTCAAAACTCTACCCTGATGTCCAATACACCTCTGTCGATCAGTATCTCTCTCGATTTGTTTGAATCAAAATATAATATACAATTGGATCGAAGAATTAAAGTGAATAATTTCTTGGTGATCTGCTGAATGCTCCCCTTTTTTCTTTTGTGTTTTTGGTCACTCTGCCTCCCTTGTAGTTGTCATTCTATGTATGCATATATACTATATGTATAATATGCTCACCATTCACCAACAGGTGTTTTATATGACTAATATAATATACAGCTTATTGATTTCCTTTTCTTTATATTGACG

mRNA sequence

CTGCATATAAATACCTTTGGTCTTAAGGGGAGGAAGACGATCCAAGCAATTTGCGTTTCTTCAGCGAAAGAAGATATTTGAAGTTATTGAAAATGACGGAGAATGGTCAGAAGAGCAAAGTTCTTATCATCGGCGGCACTGGATATATCGGAAAGTTTGTCGTGGAAGCGAGCGCTAAGGCCGCGCATCCTACGTTTGTTCTCGTCAGAGAGAGCACCATTGTCGATCCTGTAAAAGCAAAACTCTTGGAGAGTTTTAAGAATTTAGGCGTTAAATTAATCACTGGGGATCTGTATGATCATGAAGGTTTAGTGAAAGCGATAAAGCAAGTGGATGTTGTGATCTCGACTGTTGGACAAATGCAGTTGGCAGATCAGAGCAAGATCGTTGATGCGATTAAAGAGGCCGGCAATGTCAAGAGATTTTTCCCATCGGAGTTTGGAGTGGATGTGGATCGACTCCACGCAGTTGAGCCAGCAAAATCTGCACTGGCCGTAAAGGCTAATATCAGAAGGGCCATTGAGAAGGAAGGAATACCTTACACTTACGTCGTCTCCAACTGTTTTAATGGCTACTTCCTCCCCACTCTGATGCAGCCTGGACTCACCTCCCCTCCCACTCACAAGGTCGTCATTCCCGGCGACGGACACCCAAAAGCAATATTTAACTTGGAGGAAGACATCGGGACTTATACCATTAAAGCAGTGGATGATCCAAGAACTGAAAACAAAATATTGTATATCAAGCCTCCCAACAACACCTATTCTTTCAACGACCTAGTCGCTCTGTGGGAGAAGAAGATCGGCAAACCCTTGGAAAAGCTCTATGTTCCCGAACACCAAATCCTTAAGGACATTCAAGAGGCTCCACTTCCTTTGAATGTGATATTGGGTCTCAACCACTCAATATTTGTGAAAGGAGATGAGACCAATTTTGAAATTGAAGCATCTTTTGGTGTGGAGGCTTCAAAACTCTACCCTGATGTCCAATACACCTCTGTCGATCAGTATCTCTCTCGATTTGTTTGAATCAAAATATAATATACAATTGGATCGAAGAATTAAAGTGAATAATTTCTTGGTGATCTGCTGAATGCTCCCCTTTTTTCTTTTGTGTTTTTGGTCACTCTGCCTCCCTTGTAGTTGTCATTCTATGTATGCATATATACTATATGTATAATATGCTCACCATTCACCAACAGGTGTTTTATATGACTAATATAATATACAGCTTATTGATTTCCTTTTCTTTATATTGACG

Coding sequence (CDS)

ATGACGGAGAATGGTCAGAAGAGCAAAGTTCTTATCATCGGCGGCACTGGATATATCGGAAAGTTTGTCGTGGAAGCGAGCGCTAAGGCCGCGCATCCTACGTTTGTTCTCGTCAGAGAGAGCACCATTGTCGATCCTGTAAAAGCAAAACTCTTGGAGAGTTTTAAGAATTTAGGCGTTAAATTAATCACTGGGGATCTGTATGATCATGAAGGTTTAGTGAAAGCGATAAAGCAAGTGGATGTTGTGATCTCGACTGTTGGACAAATGCAGTTGGCAGATCAGAGCAAGATCGTTGATGCGATTAAAGAGGCCGGCAATGTCAAGAGATTTTTCCCATCGGAGTTTGGAGTGGATGTGGATCGACTCCACGCAGTTGAGCCAGCAAAATCTGCACTGGCCGTAAAGGCTAATATCAGAAGGGCCATTGAGAAGGAAGGAATACCTTACACTTACGTCGTCTCCAACTGTTTTAATGGCTACTTCCTCCCCACTCTGATGCAGCCTGGACTCACCTCCCCTCCCACTCACAAGGTCGTCATTCCCGGCGACGGACACCCAAAAGCAATATTTAACTTGGAGGAAGACATCGGGACTTATACCATTAAAGCAGTGGATGATCCAAGAACTGAAAACAAAATATTGTATATCAAGCCTCCCAACAACACCTATTCTTTCAACGACCTAGTCGCTCTGTGGGAGAAGAAGATCGGCAAACCCTTGGAAAAGCTCTATGTTCCCGAACACCAAATCCTTAAGGACATTCAAGAGGCTCCACTTCCTTTGAATGTGATATTGGGTCTCAACCACTCAATATTTGTGAAAGGAGATGAGACCAATTTTGAAATTGAAGCATCTTTTGGTGTGGAGGCTTCAAAACTCTACCCTGATGTCCAATACACCTCTGTCGATCAGTATCTCTCTCGATTTGTTTGA

Protein sequence

MTENGQKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGVKLITGDLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDVDRLHAVEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVVIPGDGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKPLEKLYVPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQYTSVDQYLSRFV*

Homology

[Close]

BLAST of CSPI02G20150 vs. ExPASy Swiss-Prot
Match: B2WSN0 (Eugenol synthase 2 OS=Clarkia breweri OX=36903 GN=EGS2 PE=1 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 3.6e-142
Identity = 242/308 (78.57%), Postives = 276/308 (89.61%), Query Frame = 0


Query: 5   GQKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGVKLIT 64
           G KSK+LIIGGTGYIGKF+VEAS K  HPTF LVRE+T+ DPVK KL+E F+NLGV L+ 
Sbjct: 2   GSKSKILIIGGTGYIGKFIVEASVKEGHPTFALVRETTVSDPVKGKLVEKFQNLGVSLLY 61

Query: 65  GDLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDVDRLH 124
           GDLYDH+ LVKAIKQVDVVISTVG MQ+ADQ+KI+ AIKEAGNVKRFFPSEFG DVD ++
Sbjct: 62  GDLYDHDSLVKAIKQVDVVISTVGFMQIADQTKIIAAIKEAGNVKRFFPSEFGNDVDHVN 121

Query: 125 AVEPAKS-ALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVVIPG 184
           AVEPAKS A AVKANIRRA+E EGIPYTYV SNCFNGYFLPTL+QPG T+PP  KV+IPG
Sbjct: 122 AVEPAKSVAFAVKANIRRAVEAEGIPYTYVASNCFNGYFLPTLVQPGATTPPRDKVIIPG 181

Query: 185 DGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKPLEK 244
           DG+PKAIFN EEDIGTYTIKAVDDPRT NKILY++P NN YSFN+LVALWEKKIGK LEK
Sbjct: 182 DGNPKAIFNKEEDIGTYTIKAVDDPRTLNKILYLRPSNNIYSFNELVALWEKKIGKTLEK 241

Query: 245 LYVPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQYTSV 304
           +YVPE QILKDIQEAP+P+N+ LG+NHS+FVKGD TNFEIE SFGVEAS+LYP+V+YT+V
Sbjct: 242 IYVPEEQILKDIQEAPIPINIFLGINHSVFVKGDHTNFEIEPSFGVEASELYPEVKYTTV 301

Query: 305 DQYLSRFV 312
           ++YL +FV
Sbjct: 302 EEYLDQFV 309

[Close]

BLAST of CSPI02G20150 vs. ExPASy Swiss-Prot
Match: Q9FUW6 (Phenylcoumaran benzylic ether reductase Betv6 OS=Betula pendula OX=3505 GN=BETV6 PE=1 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 2.3e-136
Identity = 234/305 (76.72%), Postives = 267/305 (87.54%), Query Frame = 0


Query: 7   KSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGVKLITGD 66
           KSK+LIIGGTGYIGKF+VEASAK+ HPTF LVREST+ DPVK KL+E FK LGV L+ GD
Sbjct: 4   KSKILIIGGTGYIGKFIVEASAKSGHPTFALVRESTVSDPVKGKLVEKFKGLGVTLLHGD 63

Query: 67  LYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDVDRLHAV 126
           LYDHE LVKA KQVDVVISTVG +QLADQ KI+ AIKEAGN+KRFFPSEFG DVDR+HAV
Sbjct: 64  LYDHESLVKAFKQVDVVISTVGHLQLADQVKIIAAIKEAGNIKRFFPSEFGNDVDRVHAV 123

Query: 127 EPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVVIPGDGH 186
           EPAK+A A KA IRR  E EGIPYTYV SN F GYFLPTL QPGLTSPP  KVVI GDG+
Sbjct: 124 EPAKTAFATKAEIRRKTEAEGIPYTYVSSNFFAGYFLPTLAQPGLTSPPREKVVIFGDGN 183

Query: 187 PKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKPLEKLYV 246
            +A+FN E+DIGTYTI+AVDDPRT NKI+YIKP  N YSFN++VALWEKKIGK LEK+YV
Sbjct: 184 ARAVFNKEDDIGTYTIRAVDDPRTLNKIVYIKPAKNIYSFNEIVALWEKKIGKTLEKIYV 243

Query: 247 PEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQYTSVDQY 306
           PE ++LKDIQE+P+P+NVIL +NHS+FVKGD TNFEIEASFGVEAS+LYPDV+YT+V++Y
Sbjct: 244 PEEKLLKDIQESPIPINVILAINHSVFVKGDHTNFEIEASFGVEASELYPDVKYTTVEEY 303

Query: 307 LSRFV 312
           L +FV
Sbjct: 304 LQQFV 308

[Close]

BLAST of CSPI02G20150 vs. ExPASy Swiss-Prot
Match: B2WSN1 (Eugenol synthase 1 OS=Petunia hybrida OX=4102 GN=EGS1 PE=1 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 2.3e-136
Identity = 232/305 (76.07%), Postives = 266/305 (87.21%), Query Frame = 0


Query: 6   QKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGVKLITG 65
           +KSK+LIIGGTGYIGKFVVEASAKA HPTFVLVREST+ DP K K++ESF N GV ++ G
Sbjct: 3   EKSKILIIGGTGYIGKFVVEASAKAGHPTFVLVRESTVSDPAKGKIVESFNNSGVTILYG 62

Query: 66  DLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDVDRLHA 125
           DLYDHE LVKAIKQVDVVISTVGQMQLADQ+KI+ AIKEAGN+KRFFPSEFG+DVD+++A
Sbjct: 63  DLYDHESLVKAIKQVDVVISTVGQMQLADQTKIIAAIKEAGNIKRFFPSEFGMDVDKVNA 122

Query: 126 VEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVVIPGDG 185
           VEPAKS  A+K  IRRAIE EGIPYTYV SNCF GYFLPTL+QPG T PP  KV+I GDG
Sbjct: 123 VEPAKSTFAIKVQIRRAIEAEGIPYTYVSSNCFAGYFLPTLVQPGATDPPRDKVIISGDG 182

Query: 186 HPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKPLEKLY 245
           + KA+FN E DIGTYTIKAVDDPRT NK LYIKPP NT SFN+LVA+WEK IGK LEK+Y
Sbjct: 183 NAKAVFNEEHDIGTYTIKAVDDPRTLNKTLYIKPPKNTLSFNELVAIWEKLIGKTLEKIY 242

Query: 246 VPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQYTSVDQ 305
           +PE QILKDI  +P+P+N+IL +NHS FVKGD+TNF IE SFGVEAS+LYPDV+YT+V++
Sbjct: 243 IPEEQILKDIATSPIPINIILAINHSTFVKGDQTNFVIEPSFGVEASELYPDVKYTTVEE 302

Query: 306 YLSRF 311
           YLS F
Sbjct: 303 YLSHF 307

[Close]

BLAST of CSPI02G20150 vs. ExPASy Swiss-Prot
Match: B6VRE8 (Phenylcoumaran benzylic ether reductase TP7 OS=Nicotiana tabacum OX=4097 GN=TP7 PE=1 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 7.3e-135
Identity = 230/306 (75.16%), Postives = 266/306 (86.93%), Query Frame = 0


Query: 6   QKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGVKLITG 65
           +KSKVLIIGGTGYIGKFVVEASAK+ HPTF LVREST+ DPVK+K++E+FKNLGV ++ G
Sbjct: 3   EKSKVLIIGGTGYIGKFVVEASAKSGHPTFALVRESTLSDPVKSKIVENFKNLGVTILHG 62

Query: 66  DLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDVDRLHA 125
           DLYDHE LVKAIKQVDVVIST+G MQL DQ K++ AIKEAGN+KRFFPSEFG+DVD+ +A
Sbjct: 63  DLYDHESLVKAIKQVDVVISTMGMMQLGDQVKLIAAIKEAGNIKRFFPSEFGMDVDKTNA 122

Query: 126 VEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVVIPGDG 185
           VEPAKSA AVK  IRRAIE EGIPYTYV  NCF GYFLPT++QPG T PP  KV+IPGDG
Sbjct: 123 VEPAKSAFAVKVQIRRAIEAEGIPYTYVSCNCFAGYFLPTMVQPGATVPPRDKVIIPGDG 182

Query: 186 HPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKPLEKLY 245
           + KA+FN E DIGTYTIKAVDDPRT NK LYIKPP NT SFN+LVA+WEK IGK LEK+Y
Sbjct: 183 NVKAVFNEEHDIGTYTIKAVDDPRTLNKTLYIKPPKNTLSFNELVAMWEKMIGKTLEKIY 242

Query: 246 VPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQYTSVDQ 305
           +PE QILKDI+ +P+PL VIL +NH+ FVKGD+TNF+IE SFGVEAS+LYPDV+YT+V+ 
Sbjct: 243 IPEEQILKDIETSPMPLPVILAINHATFVKGDQTNFKIEPSFGVEASELYPDVKYTTVED 302

Query: 306 YLSRFV 312
           YL  FV
Sbjct: 303 YLGHFV 308

[Close]

BLAST of CSPI02G20150 vs. ExPASy Swiss-Prot
Match: E1U332 (Isoflavone reductase-like protein OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 3.4e-132
Identity = 225/305 (73.77%), Postives = 264/305 (86.56%), Query Frame = 0


Query: 7   KSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGVKLITGD 66
           K+K+LIIGGTGYIGKF+VEASAK+ HPTF L RESTI DPVK K+++ FKN GV ++TGD
Sbjct: 4   KTKILIIGGTGYIGKFIVEASAKSEHPTFALARESTISDPVKGKIIQGFKNSGVTILTGD 63

Query: 67  LYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDVDRLHAV 126
           LYDHE LVKAIKQVDVVISTVGQ+QLADQ KI+ AIKEAGNVKRFFPS+FG DVDR HAV
Sbjct: 64  LYDHESLVKAIKQVDVVISTVGQLQLADQVKIIAAIKEAGNVKRFFPSDFGTDVDRCHAV 123

Query: 127 EPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVVIPGDGH 186
           EPAKS+  +K+ IRRAIE EGIPYT+V +N F GY LPTL+QP +T+PP  KV+I GDG+
Sbjct: 124 EPAKSSFEIKSQIRRAIEAEGIPYTFVSANYFAGYSLPTLVQPEVTAPPRDKVIILGDGN 183

Query: 187 PKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKPLEKLYV 246
            KA+FN E DIGTYTIKAVDD RT NKILYIKPP N YSFN+LVALWEKKIGK LEK+YV
Sbjct: 184 AKAVFNEENDIGTYTIKAVDDARTLNKILYIKPPKNIYSFNELVALWEKKIGKTLEKIYV 243

Query: 247 PEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQYTSVDQY 306
           PE Q+LK IQE+P P+N+++ +NHS FVKGD TNF+IE SFGVEAS+LYPDV+YT+V++Y
Sbjct: 244 PEEQVLKQIQESPFPINIVMAINHSAFVKGDLTNFKIEPSFGVEASELYPDVKYTTVEEY 303

Query: 307 LSRFV 312
           L +FV
Sbjct: 304 LDQFV 308

[Close]

BLAST of CSPI02G20150 vs. ExPASy TrEMBL
Match: A0A0A0LL27 (NmrA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361740 PE=4 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 1.8e-173
Identity = 307/311 (98.71%), Postives = 308/311 (99.04%), Query Frame = 0


Query: 1   MTENGQKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGV 60
           M ENGQKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTI DPVKAKLLESFKNLGV
Sbjct: 1   MAENGQKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIADPVKAKLLESFKNLGV 60

Query: 61  KLITGDLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDV 120
           K ITGDLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDV
Sbjct: 61  KFITGDLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDV 120

Query: 121 DRLHAVEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVV 180
           DRLHAVEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVV
Sbjct: 121 DRLHAVEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVV 180

Query: 181 IPGDGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKP 240
           IPGDGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKP
Sbjct: 181 IPGDGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKP 240

Query: 241 LEKLYVPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQY 300
           LEKLYVPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQY
Sbjct: 241 LEKLYVPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQY 300

Query: 301 TSVDQYLSRFV 312
           T+VDQYLSRFV
Sbjct: 301 TTVDQYLSRFV 311

[Close]

BLAST of CSPI02G20150 vs. ExPASy TrEMBL
Match: A0A5A7VHA2 (Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005640 PE=4 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.3e-166
Identity = 291/311 (93.57%), Postives = 303/311 (97.43%), Query Frame = 0


Query: 1   MTENGQKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGV 60
           M E+G+KS+VLIIGGTGYIGKFVVEASAKA HPTFVL+RESTI DP+KAKL+E+FK+LGV
Sbjct: 1   MAESGRKSRVLIIGGTGYIGKFVVEASAKAEHPTFVLIRESTIADPMKAKLVENFKSLGV 60

Query: 61  KLITGDLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDV 120
           K ITGDLYDHE LVKAIKQVDVVISTVGQMQLADQSKI+DAIKEAGNVKRFFPSEFGVDV
Sbjct: 61  KFITGDLYDHESLVKAIKQVDVVISTVGQMQLADQSKIIDAIKEAGNVKRFFPSEFGVDV 120

Query: 121 DRLHAVEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVV 180
           DRLHAVEPAKSALAVK+NIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKV+
Sbjct: 121 DRLHAVEPAKSALAVKSNIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVI 180

Query: 181 IPGDGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKP 240
           IPGDGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYI PPNNTYSFNDLVALWEKKIGKP
Sbjct: 181 IPGDGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYINPPNNTYSFNDLVALWEKKIGKP 240

Query: 241 LEKLYVPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQY 300
           LEKLYVPEHQILKDIQEAPLPLNVIL LNHSIFVKGDETNFEIE SFGVEASKLYPDVQY
Sbjct: 241 LEKLYVPEHQILKDIQEAPLPLNVILSLNHSIFVKGDETNFEIEPSFGVEASKLYPDVQY 300

Query: 301 TSVDQYLSRFV 312
           T+VDQYLSRFV
Sbjct: 301 TTVDQYLSRFV 311

[Close]

BLAST of CSPI02G20150 vs. ExPASy TrEMBL
Match: A0A1S3BB27 (isoflavone reductase-like protein OS=Cucumis melo OX=3656 GN=LOC103487978 PE=4 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.3e-166
Identity = 291/311 (93.57%), Postives = 303/311 (97.43%), Query Frame = 0


Query: 1   MTENGQKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGV 60
           M E+G+KS+VLIIGGTGYIGKFVVEASAKA HPTFVL+RESTI DP+KAKL+E+FK+LGV
Sbjct: 1   MAESGRKSRVLIIGGTGYIGKFVVEASAKAEHPTFVLIRESTIADPMKAKLVENFKSLGV 60

Query: 61  KLITGDLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDV 120
           K ITGDLYDHE LVKAIKQVDVVISTVGQMQLADQSKI+DAIKEAGNVKRFFPSEFGVDV
Sbjct: 61  KFITGDLYDHESLVKAIKQVDVVISTVGQMQLADQSKIIDAIKEAGNVKRFFPSEFGVDV 120

Query: 121 DRLHAVEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVV 180
           DRLHAVEPAKSALAVK+NIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKV+
Sbjct: 121 DRLHAVEPAKSALAVKSNIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVI 180

Query: 181 IPGDGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKP 240
           IPGDGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYI PPNNTYSFNDLVALWEKKIGKP
Sbjct: 181 IPGDGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYINPPNNTYSFNDLVALWEKKIGKP 240

Query: 241 LEKLYVPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQY 300
           LEKLYVPEHQILKDIQEAPLPLNVIL LNHSIFVKGDETNFEIE SFGVEASKLYPDVQY
Sbjct: 241 LEKLYVPEHQILKDIQEAPLPLNVILSLNHSIFVKGDETNFEIEPSFGVEASKLYPDVQY 300

Query: 301 TSVDQYLSRFV 312
           T+VDQYLSRFV
Sbjct: 301 TTVDQYLSRFV 311

[Close]

BLAST of CSPI02G20150 vs. ExPASy TrEMBL
Match: A0A6J1GJH4 (isoflavone reductase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111454417 PE=4 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 9.8e-151
Identity = 265/306 (86.60%), Postives = 286/306 (93.46%), Query Frame = 0


Query: 6   QKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGVKLITG 65
           +KSKVLIIGGTGYIGKFVVEASAKA HPTF L+REST+ DPVKAKL+E+FKNLGVKLITG
Sbjct: 3   EKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLITG 62

Query: 66  DLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDVDRLHA 125
           DLYDHE LVKAIK+VDVVIST+GQMQLADQ KI+ AIKEA NVKRFFPSEFG+DVDRLHA
Sbjct: 63  DLYDHESLVKAIKEVDVVISTLGQMQLADQVKIIAAIKEACNVKRFFPSEFGMDVDRLHA 122

Query: 126 VEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVVIPGDG 185
           VEPAKSALA+KA IRRAIE+EGIPYTYV SNCFNGYFLPTL QPGLT+PP  KVVI GDG
Sbjct: 123 VEPAKSALAIKAQIRRAIEREGIPYTYVSSNCFNGYFLPTLSQPGLTAPPRDKVVISGDG 182

Query: 186 HPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKPLEKLY 245
           HPK IFNLEEDIGTYTIKAVDDP+TENKILYI+PP NTYSFNDLVALWEKKI K LEK+Y
Sbjct: 183 HPKVIFNLEEDIGTYTIKAVDDPKTENKILYIRPPKNTYSFNDLVALWEKKIDKVLEKIY 242

Query: 246 VPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQYTSVDQ 305
           VPE QILKDIQEAPLPLNVIL LNHS+FVKGD+TNFEIEASFGVEAS LYP+V+YT+VD+
Sbjct: 243 VPEDQILKDIQEAPLPLNVILALNHSVFVKGDQTNFEIEASFGVEASALYPEVRYTTVDE 302

Query: 306 YLSRFV 312
           YLSRFV
Sbjct: 303 YLSRFV 308

[Close]

BLAST of CSPI02G20150 vs. ExPASy TrEMBL
Match: A0A6J1KTK3 (isoflavone reductase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111496281 PE=4 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 3.7e-150
Identity = 265/306 (86.60%), Postives = 285/306 (93.14%), Query Frame = 0


Query: 6   QKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGVKLITG 65
           +KSKVLIIGGTGYIGKFVVEASAKA HPTF LVREST+ DPVKAKL+E+FKNLGVKLITG
Sbjct: 3   EKSKVLIIGGTGYIGKFVVEASAKAGHPTFALVRESTVADPVKAKLVENFKNLGVKLITG 62

Query: 66  DLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDVDRLHA 125
           DLYDHE LVKAIK+VDVVIST+GQMQLADQ KI+ AIKEAGNVKRFFPSEFG+DVDRLHA
Sbjct: 63  DLYDHESLVKAIKEVDVVISTLGQMQLADQIKIIAAIKEAGNVKRFFPSEFGMDVDRLHA 122

Query: 126 VEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVVIPGDG 185
           VEPAKS LAVKA IRRAIE+EGIPYTYV SN FNGYFLPTL QPGLT+PP  KVVIPGDG
Sbjct: 123 VEPAKSVLAVKAQIRRAIEREGIPYTYVSSNYFNGYFLPTLSQPGLTAPPRDKVVIPGDG 182

Query: 186 HPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKPLEKLY 245
           HPKA+FNLEEDIGTYTIKAVDDP+TENKILYI+PP NTYSFNDLVALWEKKI K LEK+Y
Sbjct: 183 HPKAVFNLEEDIGTYTIKAVDDPKTENKILYIRPPKNTYSFNDLVALWEKKIDKVLEKIY 242

Query: 246 VPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQYTSVDQ 305
           VPE QILKDIQ+APLPLNVIL LNHS FVKGD+TNFEIE SFGVEAS LYP+V+YT+VD+
Sbjct: 243 VPEDQILKDIQDAPLPLNVILALNHSAFVKGDQTNFEIEPSFGVEASALYPEVRYTTVDE 302

Query: 306 YLSRFV 312
           YLSRFV
Sbjct: 303 YLSRFV 308

[Close]

BLAST of CSPI02G20150 vs. NCBI nr
Match: XP_004152692.1 (phenylcoumaran benzylic ether reductase Betv6 [Cucumis sativus] >KGN62610.1 hypothetical protein Csa_022559 [Cucumis sativus])

HSP 1 Score: 618.2 bits (1593), Expect = 3.8e-173
Identity = 307/311 (98.71%), Postives = 308/311 (99.04%), Query Frame = 0


Query: 1   MTENGQKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGV 60
           M ENGQKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTI DPVKAKLLESFKNLGV
Sbjct: 1   MAENGQKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIADPVKAKLLESFKNLGV 60

Query: 61  KLITGDLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDV 120
           K ITGDLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDV
Sbjct: 61  KFITGDLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDV 120

Query: 121 DRLHAVEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVV 180
           DRLHAVEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVV
Sbjct: 121 DRLHAVEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVV 180

Query: 181 IPGDGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKP 240
           IPGDGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKP
Sbjct: 181 IPGDGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKP 240

Query: 241 LEKLYVPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQY 300
           LEKLYVPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQY
Sbjct: 241 LEKLYVPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQY 300

Query: 301 TSVDQYLSRFV 312
           T+VDQYLSRFV
Sbjct: 301 TTVDQYLSRFV 311

[Close]

BLAST of CSPI02G20150 vs. NCBI nr
Match: XP_008444728.1 (PREDICTED: isoflavone reductase-like protein [Cucumis melo] >KAA0065225.1 isoflavone reductase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 595.5 bits (1534), Expect = 2.6e-166
Identity = 291/311 (93.57%), Postives = 303/311 (97.43%), Query Frame = 0


Query: 1   MTENGQKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGV 60
           M E+G+KS+VLIIGGTGYIGKFVVEASAKA HPTFVL+RESTI DP+KAKL+E+FK+LGV
Sbjct: 1   MAESGRKSRVLIIGGTGYIGKFVVEASAKAEHPTFVLIRESTIADPMKAKLVENFKSLGV 60

Query: 61  KLITGDLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDV 120
           K ITGDLYDHE LVKAIKQVDVVISTVGQMQLADQSKI+DAIKEAGNVKRFFPSEFGVDV
Sbjct: 61  KFITGDLYDHESLVKAIKQVDVVISTVGQMQLADQSKIIDAIKEAGNVKRFFPSEFGVDV 120

Query: 121 DRLHAVEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVV 180
           DRLHAVEPAKSALAVK+NIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKV+
Sbjct: 121 DRLHAVEPAKSALAVKSNIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVI 180

Query: 181 IPGDGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKP 240
           IPGDGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYI PPNNTYSFNDLVALWEKKIGKP
Sbjct: 181 IPGDGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYINPPNNTYSFNDLVALWEKKIGKP 240

Query: 241 LEKLYVPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQY 300
           LEKLYVPEHQILKDIQEAPLPLNVIL LNHSIFVKGDETNFEIE SFGVEASKLYPDVQY
Sbjct: 241 LEKLYVPEHQILKDIQEAPLPLNVILSLNHSIFVKGDETNFEIEPSFGVEASKLYPDVQY 300

Query: 301 TSVDQYLSRFV 312
           T+VDQYLSRFV
Sbjct: 301 TTVDQYLSRFV 311

[Close]

BLAST of CSPI02G20150 vs. NCBI nr
Match: XP_038885595.1 (eugenol synthase 2-like [Benincasa hispida])

HSP 1 Score: 573.5 bits (1477), Expect = 1.1e-159
Identity = 280/311 (90.03%), Postives = 298/311 (95.82%), Query Frame = 0


Query: 1   MTENGQKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGV 60
           M E+ +KS+VLIIGGTGYIGKFVVEASAKA HPTFVLVRESTI DPVKAKL+E+FKNLGV
Sbjct: 1   MAEDNRKSRVLIIGGTGYIGKFVVEASAKAGHPTFVLVRESTIADPVKAKLVENFKNLGV 60

Query: 61  KLITGDLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDV 120
           KLITGDLYD E LVKAIK+VDVVISTVGQMQLADQSKI+DAIKEAGNVKRFFPSEFG+DV
Sbjct: 61  KLITGDLYDRESLVKAIKEVDVVISTVGQMQLADQSKIIDAIKEAGNVKRFFPSEFGLDV 120

Query: 121 DRLHAVEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVV 180
           DRL+AVEPAKSALA+KA IRRAIE+EGIPYTYV SNCFNGYFLPTLMQPGLTSPPT KV+
Sbjct: 121 DRLNAVEPAKSALAIKAEIRRAIEREGIPYTYVSSNCFNGYFLPTLMQPGLTSPPTDKVI 180

Query: 181 IPGDGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKP 240
           IPGDGHPKAIFNLEEDIG+YTIKAVDDPRTENKILYI PPNNTYSFNDLVALWEKKIGKP
Sbjct: 181 IPGDGHPKAIFNLEEDIGSYTIKAVDDPRTENKILYINPPNNTYSFNDLVALWEKKIGKP 240

Query: 241 LEKLYVPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQY 300
           LEKLYVPEHQIL+DIQEAPLP+NVIL LNHSIFVKGDETNFEIE SFGVEAS LYPDV+Y
Sbjct: 241 LEKLYVPEHQILRDIQEAPLPINVILALNHSIFVKGDETNFEIEPSFGVEASTLYPDVKY 300

Query: 301 TSVDQYLSRFV 312
           T+VD+YLSRFV
Sbjct: 301 TTVDEYLSRFV 311

[Close]

BLAST of CSPI02G20150 vs. NCBI nr
Match: KAG7020505.1 (hypothetical protein SDJN02_17190 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 543.5 bits (1399), Expect = 1.2e-150
Identity = 266/306 (86.93%), Postives = 286/306 (93.46%), Query Frame = 0


Query: 6   QKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGVKLITG 65
           +KSKVLIIGGTGYIGKFVVEASAKA H TF L+REST+ DPVKAKL+E+FKNLGVKLITG
Sbjct: 3   EKSKVLIIGGTGYIGKFVVEASAKAGHTTFALIRESTVADPVKAKLVENFKNLGVKLITG 62

Query: 66  DLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDVDRLHA 125
           DLYDHE LVKAIK+VDVVIST+GQMQLADQ KI+ AIKEAGNVKRFFPSEFG+DVDRLHA
Sbjct: 63  DLYDHESLVKAIKEVDVVISTLGQMQLADQVKIIAAIKEAGNVKRFFPSEFGMDVDRLHA 122

Query: 126 VEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVVIPGDG 185
           VEPAKSALAVKA IRRAIE+EGIPYTYV SNCFNGYFLPTL QPGLT+PP  KVVI GDG
Sbjct: 123 VEPAKSALAVKAQIRRAIEREGIPYTYVSSNCFNGYFLPTLSQPGLTAPPRDKVVISGDG 182

Query: 186 HPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKPLEKLY 245
           HPK IFNLEEDIGTYTIKAVDDP+TENKILYI+PP NTYSFNDLVALWEKKI K LEK+Y
Sbjct: 183 HPKVIFNLEEDIGTYTIKAVDDPKTENKILYIRPPKNTYSFNDLVALWEKKIDKVLEKIY 242

Query: 246 VPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQYTSVDQ 305
           VPE QILKDIQEAPLPLNVIL LNHS+FVKGD+TNFEIEASFGVEAS LYP+V+YT+VD+
Sbjct: 243 VPEDQILKDIQEAPLPLNVILALNHSVFVKGDQTNFEIEASFGVEASALYPEVRYTTVDE 302

Query: 306 YLSRFV 312
           YLSRFV
Sbjct: 303 YLSRFV 308

[Close]

BLAST of CSPI02G20150 vs. NCBI nr
Match: XP_022951674.1 (isoflavone reductase-like protein [Cucurbita moschata])

HSP 1 Score: 542.7 bits (1397), Expect = 2.0e-150
Identity = 265/306 (86.60%), Postives = 286/306 (93.46%), Query Frame = 0


Query: 6   QKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGVKLITG 65
           +KSKVLIIGGTGYIGKFVVEASAKA HPTF L+REST+ DPVKAKL+E+FKNLGVKLITG
Sbjct: 3   EKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLITG 62

Query: 66  DLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDVDRLHA 125
           DLYDHE LVKAIK+VDVVIST+GQMQLADQ KI+ AIKEA NVKRFFPSEFG+DVDRLHA
Sbjct: 63  DLYDHESLVKAIKEVDVVISTLGQMQLADQVKIIAAIKEACNVKRFFPSEFGMDVDRLHA 122

Query: 126 VEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVVIPGDG 185
           VEPAKSALA+KA IRRAIE+EGIPYTYV SNCFNGYFLPTL QPGLT+PP  KVVI GDG
Sbjct: 123 VEPAKSALAIKAQIRRAIEREGIPYTYVSSNCFNGYFLPTLSQPGLTAPPRDKVVISGDG 182

Query: 186 HPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKPLEKLY 245
           HPK IFNLEEDIGTYTIKAVDDP+TENKILYI+PP NTYSFNDLVALWEKKI K LEK+Y
Sbjct: 183 HPKVIFNLEEDIGTYTIKAVDDPKTENKILYIRPPKNTYSFNDLVALWEKKIDKVLEKIY 242

Query: 246 VPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQYTSVDQ 305
           VPE QILKDIQEAPLPLNVIL LNHS+FVKGD+TNFEIEASFGVEAS LYP+V+YT+VD+
Sbjct: 243 VPEDQILKDIQEAPLPLNVILALNHSVFVKGDQTNFEIEASFGVEASALYPEVRYTTVDE 302

Query: 306 YLSRFV 312
           YLSRFV
Sbjct: 303 YLSRFV 308

[Close]

BLAST of CSPI02G20150 vs. TAIR 10
Match: AT1G75280.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 449.5 bits (1155), Expect = 2.2e-126
Identity = 221/307 (71.99%), Postives = 255/307 (83.06%), Query Frame = 0


Query: 6   QKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGVKLITG 65
           +KSK+L+IGGTGYIGKF+VEASAKA H TF LVRE+T+ DPVK K ++SFK+LGV ++ G
Sbjct: 4   EKSKILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHG 63

Query: 66  DLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDVDRLHA 125
           DL DHE LVKAIKQVDVVISTVG MQ+ DQ+KI+ AIKEAGNVKRF PSEFGVDVDR  A
Sbjct: 64  DLNDHESLVKAIKQVDVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDRTSA 123

Query: 126 VEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQ--PGLTSPPTHKVVIPG 185
           VEPAKSA A K  IRR IE EGIPYTY V+ CF GY+LPTL+Q  PGLTSPP  KV I G
Sbjct: 124 VEPAKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVTILG 183

Query: 186 DGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKPLEK 245
           DG+ KA+ N EEDI  YTIKAVDDPRT NKILYIKP NNT S N++V LWEKKIGK LEK
Sbjct: 184 DGNAKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIGKSLEK 243

Query: 246 LYVPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQYTSV 305
            ++PE Q+LK IQE+P+P+NV+L +NH++FV GD TN  IE SFGVEAS+LYPDV+YTSV
Sbjct: 244 THLPEEQLLKSIQESPIPINVVLSINHAVFVNGD-TNISIEPSFGVEASELYPDVKYTSV 303

Query: 306 DQYLSRF 311
           D+YLS F
Sbjct: 304 DEYLSYF 309

[Close]

BLAST of CSPI02G20150 vs. TAIR 10
Match: AT4G39230.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 432.6 bits (1111), Expect = 2.8e-121
Identity = 211/305 (69.18%), Postives = 246/305 (80.66%), Query Frame = 0


Query: 7   KSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGVKLITGD 66
           KSK+L IGGTGYIGK++VEASA++ HPT VLVR ST+  P ++  +E+FKNLGV+ + GD
Sbjct: 4   KSKILFIGGTGYIGKYIVEASARSGHPTLVLVRNSTLTSPSRSSTIENFKNLGVQFLLGD 63

Query: 67  LYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDVDRLHAV 126
           L DH  LV +IKQ DVVISTVG   L  Q KI+ AIKEAGNVKRFFPSEFG DVDR+  V
Sbjct: 64  LDDHTSLVNSIKQADVVISTVGHSLLGHQYKIISAIKEAGNVKRFFPSEFGNDVDRVFTV 123

Query: 127 EPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVVIPGDGH 186
           EPAKSA A KA IRR IE EGIPYTYV  N F GYFLPTL QPG TS P  KV++ GDG+
Sbjct: 124 EPAKSAYATKAKIRRTIEAEGIPYTYVSCNFFAGYFLPTLAQPGATSAPRDKVIVLGDGN 183

Query: 187 PKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKPLEKLYV 246
           PKA+FN EEDIGTYTI AVDDPRT NKILYI+PP NTYSFNDLV+LWE KIGK LE++YV
Sbjct: 184 PKAVFNKEEDIGTYTINAVDDPRTLNKILYIRPPMNTYSFNDLVSLWENKIGKTLERIYV 243

Query: 247 PEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQYTSVDQY 306
           PE Q+LK I E+  PLNV+L L H +FVKG  T+FEIE SFGVEAS+LYPDV+YT+VD+ 
Sbjct: 244 PEEQLLKQIIESSPPLNVMLSLCHCVFVKGGHTSFEIEPSFGVEASELYPDVKYTTVDEI 303

Query: 307 LSRFV 312
           L+++V
Sbjct: 304 LNQYV 308

[Close]

BLAST of CSPI02G20150 vs. TAIR 10
Match: AT1G75290.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 425.6 bits (1093), Expect = 3.4e-119
Identity = 205/307 (66.78%), Postives = 247/307 (80.46%), Query Frame = 0


Query: 6   QKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGVKLITG 65
           +KSK+L+IGGTG+IGK ++EAS KA H T  LVRE+++ DP K K +++FK+ GV L+ G
Sbjct: 4   EKSKILVIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLHG 63

Query: 66  DLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDVDRLHA 125
           DL DHE LVKAIKQ DVVISTVG MQ+ DQ+KI+ AIKEAGNVKRF PSEFG+DVD+  A
Sbjct: 64  DLNDHESLVKAIKQADVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGMDVDKSSA 123

Query: 126 VEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQ--PGLTSPPTHKVVIPG 185
           VEPAKSA   K   RR IE EGIPYTY+V+N F GY+LPTL+Q  PGLTSPP  KV I G
Sbjct: 124 VEPAKSAFGRKLQTRRDIEAEGIPYTYLVTNYFAGYYLPTLVQLEPGLTSPPRDKVKIFG 183

Query: 186 DGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKPLEK 245
           DG+ KA+ N EEDI  YTIKAVDDPRT NK LYI PPNNT S N++V LWEKKIGK +EK
Sbjct: 184 DGNVKAVINKEEDIAAYTIKAVDDPRTLNKTLYINPPNNTLSMNEIVTLWEKKIGKSVEK 243

Query: 246 LYVPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQYTSV 305
           +Y+ E QI K IQE+P+P NV+L +NH++FVKGD+TNF IE SFG EAS+LYPD++YTS+
Sbjct: 244 IYMSEEQIFKSIQESPVPFNVLLSINHAVFVKGDQTNFTIEPSFGFEASELYPDIKYTSI 303

Query: 306 DQYLSRF 311
           D+YLS F
Sbjct: 304 DEYLSYF 310

[Close]

BLAST of CSPI02G20150 vs. TAIR 10
Match: AT1G75300.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 394.0 bits (1011), Expect = 1.1e-109
Identity = 198/321 (61.68%), Postives = 243/321 (75.70%), Query Frame = 0


Query: 6   QKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGVKLITG 65
           +KSK+L+IGGTGY+G+F+VE SAKA +PTF LVRE+++ DPVK+K ++SFK+LGV ++ G
Sbjct: 4   EKSKILVIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLGVTILHG 63

Query: 66  DLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDVDRLHA 125
           DL DHE LVKAIKQVDVVIST+G  Q+ DQ+KI+ AIKEAGNVKRF P+EFG+DV+R  A
Sbjct: 64  DLNDHESLVKAIKQVDVVISTIGHKQIFDQTKIISAIKEAGNVKRFLPAEFGIDVERTSA 123

Query: 126 VEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQ--PGLTS---------- 185
           VEPAKS  A K  IRRAIE EGIPYTYVVSNC  G++L TL+Q   GL S          
Sbjct: 124 VEPAKSLFAGKVQIRRAIEAEGIPYTYVVSNCSAGFYLRTLLQFESGLISHTRDKAIIFG 183

Query: 186 ----PPTHKVVIPGDGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDL 245
               PP  KV I GDG+ K + N EED+  Y IKAVDD RT NK LYI PPNN  S N++
Sbjct: 184 DKNVPPRDKVTILGDGNAKVVINKEEDVAAYMIKAVDDLRTLNKTLYISPPNNILSMNEM 243

Query: 246 VALWEKKIGKPLEKLYVPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGV 305
           V LWEKKIGK LEK ++ E QILK IQ   +P++V   +NH++FVKGD+T+F IE  FG 
Sbjct: 244 VTLWEKKIGKSLEKTHISEEQILKSIQ---VPIDVFKSINHAVFVKGDQTSFTIEPWFGE 303

Query: 306 EASKLYPDVQYTSVDQYLSRF 311
           EAS LYPDV+YTS+D+YLS+F
Sbjct: 304 EASVLYPDVKYTSIDEYLSQF 321

[Close]

BLAST of CSPI02G20150 vs. TAIR 10
Match: AT1G19540.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 364.8 bits (935), Expect = 7.1e-101
Identity = 190/308 (61.69%), Postives = 231/308 (75.00%), Query Frame = 0


Query: 8   SKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIVDPVKAKLLESFKNLGVKLITGDL 67
           SK+L+IG TG IGK +VE SAK+ H TF LVRE+++ DPVKA+L+E FK+LGV ++ G L
Sbjct: 3   SKILVIGATGLIGKVLVEESAKSGHATFALVREASLSDPVKAQLVERFKDLGVTILYGSL 62

Query: 68  YDHEGLVKAIKQVDVVISTVG--QMQLADQSKIVDAIKEAGNVKRFFPSEFGVDVDRLHA 127
            D E LVKAIKQVDVVIS VG  Q ++ +Q+ I+DAIKE+GNVKRF PSEFG DVDR  A
Sbjct: 63  SDKESLVKAIKQVDVVISAVGRFQTEILNQTNIIDAIKESGNVKRFLPSEFGNDVDRTVA 122

Query: 128 VEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQ--PGLTSPPTHKVVIPG 187
           +EP  S    KA IRRAIE   IPYTYVVS CF G F+P L Q    L SPP  KV I  
Sbjct: 123 IEPTLSEFITKAQIRRAIEAAKIPYTYVVSGCFAGLFVPCLGQCHLRLRSPPRDKVSIYD 182

Query: 188 DGHPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKPLEK 247
            G+ KAI N EEDI  YT+KAVDDPRT NKILYI PPN   S ND+V LWE+KIGK LEK
Sbjct: 183 TGNGKAIVNTEEDIVAYTLKAVDDPRTLNKILYIHPPNYIVSQNDMVGLWEEKIGKTLEK 242

Query: 248 LYVPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQYTSV 307
            YV E ++LK IQE+  P++ ++GL H+I VK D T+F I+ SFGVEAS+LYP+V+YTSV
Sbjct: 243 TYVSEEELLKTIQESKPPMDFLVGLIHTILVKSDFTSFTIDPSFGVEASELYPEVKYTSV 302

Query: 308 DQYLSRFV 312
           D++L+RF+
Sbjct: 303 DEFLNRFI 310

The following BLAST results are available for this feature:

BLAST of CSPI02G20150 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B2WSN0	3.6e-142	78.57	Eugenol synthase 2 OS=Clarkia breweri OX=36903 GN=EGS2 PE=1 SV=1	[more]
Q9FUW6	2.3e-136	76.72	Phenylcoumaran benzylic ether reductase Betv6 OS=Betula pendula OX=3505 GN=BETV6...	[more]
B2WSN1	2.3e-136	76.07	Eugenol synthase 1 OS=Petunia hybrida OX=4102 GN=EGS1 PE=1 SV=1	[more]
B6VRE8	7.3e-135	75.16	Phenylcoumaran benzylic ether reductase TP7 OS=Nicotiana tabacum OX=4097 GN=TP7 ...	[more]
E1U332	3.4e-132	73.77	Isoflavone reductase-like protein OS=Olea europaea OX=4146 PE=1 SV=1	[more]

BLAST of CSPI02G20150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LL27	1.8e-173	98.71	NmrA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361740 PE=4 S...	[more]
A0A5A7VHA2	1.3e-166	93.57	Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A1S3BB27	1.3e-166	93.57	isoflavone reductase-like protein OS=Cucumis melo OX=3656 GN=LOC103487978 PE=4 S...	[more]
A0A6J1GJH4	9.8e-151	86.60	isoflavone reductase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111454417 ...	[more]
A0A6J1KTK3	3.7e-150	86.60	isoflavone reductase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111496281 PE...	[more]

BLAST of CSPI02G20150 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152692.1	3.8e-173	98.71	phenylcoumaran benzylic ether reductase Betv6 [Cucumis sativus] >KGN62610.1 hypo...	[more]
XP_008444728.1	2.6e-166	93.57	PREDICTED: isoflavone reductase-like protein [Cucumis melo] >KAA0065225.1 isofla...	[more]
XP_038885595.1	1.1e-159	90.03	eugenol synthase 2-like [Benincasa hispida]	[more]
KAG7020505.1	1.2e-150	86.93	hypothetical protein SDJN02_17190 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022951674.1	2.0e-150	86.60	isoflavone reductase-like protein [Cucurbita moschata]	[more]

BLAST of CSPI02G20150 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G75280.1	2.2e-126	71.99	NmrA-like negative transcriptional regulator family protein	[more]
AT4G39230.1	2.8e-121	69.18	NmrA-like negative transcriptional regulator family protein	[more]
AT1G75290.1	3.4e-119	66.78	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G75300.1	1.1e-109	61.68	NmrA-like negative transcriptional regulator family protein	[more]
AT1G19540.1	7.1e-101	61.69	NmrA-like negative transcriptional regulator family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.720		coord: 2..208 e-value: 1.5E-97 score: 328.3
None	No IPR available	GENE3D	3.90.25.10		coord: 68..218 e-value: 1.5E-97 score: 328.3
None	No IPR available	PANTHER	PTHR43349	PINORESINOL REDUCTASE-RELATED	coord: 1..222
None	No IPR available	PANTHER	PTHR43349:SF76	ISOFLAVONE REDUCTASE HOMOLOG P3-RELATED	coord: 1..222
IPR008030	NmrA-like domain	PFAM	PF05368	NmrA	coord: 1..185 e-value: 9.5E-55 score: 185.6
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 4..181

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G20150.1	CSPI02G20150.1	mRNA
CSPI02G20150.2	CSPI02G20150.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009807	lignan biosynthetic process
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0016491	oxidoreductase activity