CSPI02G20010 (gene) Cucumber (PI 183967) v1

BLAST of CSPI02G20010 vs. ExPASy Swiss-Prot
Match: Q8RXN5 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Arabidopsis thaliana OX=3702 GN=TYW1 PE=2 SV=1)

HSP 1 Score: 965.7 bits (2495), Expect = 2.6e-280
Identity = 468/640 (73.12%), Postives = 536/640 (83.75%), Query Frame = 0

Query: 4   SSRSAKLTILALLSASTLYCFYKSRRLRKLK-LSLNP--TLPSRKPKLFFISQTGTSKAL 63
           SS   +L  +ALLSA+T YC +K RRL+ LK LSLNP  TL + + K+FFISQTGT+KAL
Sbjct: 5   SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64

Query: 64  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 123
           A RL  +  SN + FD+VDP  YEPEDL KETLVLF+ASTW+ G PP + +FL NWL ES
Sbjct: 65  AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124

Query: 124 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 183
           AEDFRVGSLLLS CKF+VFGVGSR YGE++NAVA++ S R+  LG  E++P+GEGDVD G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184

Query: 184 DIDKCFDDWSRKLLKVMKFDA--EGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVD 243
           ++D+ F DW   +++V+K  +  E NGV    G   D        DEE++D+  +  IVD
Sbjct: 185 ELDRAFQDWCDGVIRVLKGGSAQETNGVSQQIGAVEDDLEYYDSTDEEDEDNDADGGIVD 244

Query: 244 LEDIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKS 303
           LEDIAGK PS+++  +  T  K++GKK MVTPVIRASL KQGYKIIGSHSGVKICRWTKS
Sbjct: 245 LEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICRWTKS 304

Query: 304 QLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIV 363
           QLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MD+P  IV
Sbjct: 305 QLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEPSVIV 364

Query: 364 NSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRI 423
             A+DLH  MIKQMKGVPGVT EKL+EGL+PRHCALSLVGEPIMYPEIN LVDELH RRI
Sbjct: 365 KGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELHGRRI 424

Query: 424 STFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEK 483
           STFLVTNAQFP+KI ++KP+TQLYVSVDAATKESLKAIDRPLF DFWERFIDSLKAL EK
Sbjct: 425 STFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDSLKALQEK 484

Query: 484 QQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSD 543
           QQRTVYRLTLVKGWNTE++DAY NLFSIG+PDF+EIKGVTYCGSS TSKLTMENVPWH+D
Sbjct: 485 QQRTVYRLTLVKGWNTEELDAYFNLFSIGKPDFIEIKGVTYCGSSATSKLTMENVPWHTD 544

Query: 544 VKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKP 603
           VK+FSEAL+ KS GEYEVACEH HSCCVLL +T+KF+VDG+WFTWIDY+KFHDLVASG+P
Sbjct: 545 VKAFSEALSLKSNGEYEVACEHAHSCCVLLGRTEKFKVDGKWFTWIDYEKFHDLVASGEP 604

Query: 604 FDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKP 639
           F S DYMA TPSWAVYG+ EGGFDP Q RY+KER+H PKP
Sbjct: 605 FTSTDYMAQTPSWAVYGAQEGGFDPGQLRYKKERNHPPKP 642

BLAST of CSPI02G20010 vs. ExPASy Swiss-Prot
Match: Q8H8N3 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Oryza sativa subsp. japonica OX=39947 GN=TYW1 PE=2 SV=1)

HSP 1 Score: 891.0 bits (2301), Expect = 8.2e-258
Identity = 438/641 (68.33%), Postives = 511/641 (79.72%), Query Frame = 0

Query: 4   SSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNP--TLPSRKPKLFFISQTGTSKALA 63
           +S ++ L +L LLS+S+++  YKS RLR+      P    P+  P L + S TGTSKALA
Sbjct: 12  ASTTSHLALLLLLSSSSVFFLYKSLRLRRNNPPSPPPGQGPAPTPTLLYASATGTSKALA 71

Query: 64  HRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSESA 123
             L   L   G+     D   ++P+DL    L+L V  T + G PPP A FLA WL ESA
Sbjct: 72  AGLSRRLAEAGVTAHPADAAAFDPDDLPSLPLLLLVLPTHDGGAPPPAAAFLARWLEESA 131

Query: 124 EDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGGD 183
            DFR G+ LLS  +F+VFGVGSR YGETFNA AR FS+ L+ALGA E++ +GEGDVDGGD
Sbjct: 132 ADFRAGAALLSGLRFAVFGVGSRAYGETFNAAARSFSRWLRALGAAEVVAVGEGDVDGGD 191

Query: 184 IDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDI----V 243
           ++  F++W  ++++V+K      G E+  G  G+SD     E+EE DDD  EE++    V
Sbjct: 192 LEVVFEEWCGRVVRVVK------GEEIGEGHNGESDGFDELEEEESDDDDDEEEVDGGEV 251

Query: 244 DLEDIAGKGP-SRKSTNIVE---TNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKIC 303
           D+EDIAGK P +R+    VE   +NG  NG + MVTP+IR SLEKQGYKIIGSHSGVKIC
Sbjct: 252 DMEDIAGKAPAARRRNGKVEGALSNGGENGVRDMVTPIIRTSLEKQGYKIIGSHSGVKIC 311

Query: 304 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDD 363
           RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+W+MDD
Sbjct: 312 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDD 371

Query: 364 PLEIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDEL 423
           PL+IVN+AID HTKM+KQMKGVPGV  E+L EGLSPRHCALSLVGEPIMYPEIN L+DEL
Sbjct: 372 PLDIVNAAIDQHTKMVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEINVLIDEL 431

Query: 424 HRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLK 483
           HRR ISTFLVTNAQFPDKIK LKP+TQLYVSVDAATKESLKA+DRPLF DFWERF+DSLK
Sbjct: 432 HRRHISTFLVTNAQFPDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLK 491

Query: 484 ALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENV 543
           +L++K QRTVYRLTLVKGWN E+ID Y+ L S+G+PDF+EIKGVTYCGSS TSKLTMENV
Sbjct: 492 SLHDKDQRTVYRLTLVKGWNAEEIDGYAKLLSLGQPDFIEIKGVTYCGSSATSKLTMENV 551

Query: 544 PWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLV 603
           PWHSDVK FSEALA KS G YEVACEH HSCCVLLAK DKF+++G+W TWIDYD+FH+LV
Sbjct: 552 PWHSDVKDFSEALALKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHELV 611

Query: 604 ASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHH 635
            SGKPF S+DYMA TPSWAVYG++EGGFDPDQSRY+KER H
Sbjct: 612 TSGKPFRSQDYMALTPSWAVYGAEEGGFDPDQSRYKKERRH 646

BLAST of CSPI02G20010 vs. ExPASy Swiss-Prot
Match: Q2KHP8 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Xenopus laevis OX=8355 GN=tyw1 PE=2 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 1.3e-178
Identity = 326/652 (50.00%), Postives = 429/652 (65.80%), Query Frame = 0

Query: 47  KLFFISQTGTSKALAHRLLNVLNSNGLVFDLVDPKDYEPED-LFKET----LVLFVASTW 106
           K+F+ SQTGTSK  AH L   +   GL  +L++ K+Y+P+D L +ET    + +F+ +T+
Sbjct: 84  KIFYGSQTGTSKGFAHLLAEEVTLLGLPVELINMKEYDPDDNLVEETTSKNICVFLVATY 143

Query: 107 EDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRL 166
            DG PP  A++   WL E++ DFR G   L   +++VFG+G+ VY   +N V ++  K L
Sbjct: 144 TDGKPPESAEWFCKWLEEASNDFRFGKTYLKGMRYAVFGLGNSVYSTHYNTVGKNIDKWL 203

Query: 167 KALGAKEILPIGEGDVD-----GGDIDKCFDDWSRKLLKVMKFDAEGNGVELCSG----- 226
             L A  ++   EGD +      G I+  F+ W RK L  +K    G   + CSG     
Sbjct: 204 WMLSANRVMTRAEGDCNVVKSKHGSIESDFEAWKRKFLNRLKALLSGE-KKPCSGKCKKG 263

Query: 227 ---IAGDSDAVSVEEDEEEDDDLGEED------------------------IVDLEDIAG 286
                  S   SVEE+EEE++   E +                        ++D+ED+ G
Sbjct: 264 KCKSKKKSSIESVEEEEEEEEKHSEHEDTEDDTFETSSDSEPEEHGEPGSGLIDVEDL-G 323

Query: 287 KGPS--RKSTNIVETNGKLNGKKV-------------MVTPVIRASLEKQGYKIIGSHSG 346
           K  S  +KS    ++N +L GK +             M+TP ++ +L KQGY++IGSHSG
Sbjct: 324 KAMSNMKKSKREHDSNTEL-GKSLQGDEAKEKEEPREMITPALQEALTKQGYRLIGSHSG 383

Query: 347 VKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQW 406
           VK+CRWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W
Sbjct: 384 VKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRW 443

Query: 407 EMDDPLEIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTL 466
           +MD P  I+  AI  H  MIKQ KGVPGV  E+ EEGL+ +HCALSLVGEPIMYPEINT 
Sbjct: 444 KMDQPEMILEEAILNHQNMIKQFKGVPGVKPERFEEGLAVKHCALSLVGEPIMYPEINTF 503

Query: 467 VDELHRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI 526
           +  LH + IS+FLVTNAQFP++I+ L+PVTQLYVSVDA+TK+SLK IDRPLF DFW+RFI
Sbjct: 504 LRLLHNQHISSFLVTNAQFPEEIRSLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFI 563

Query: 527 DSLKALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLT 586
           DSLKAL EKQQRTVYRLTLVK WN +++ AY++L ++G PDF+E+KGVTYCG S+ S LT
Sbjct: 564 DSLKALAEKQQRTVYRLTLVKAWNVDELKAYADLVALGLPDFIEVKGVTYCGESSASNLT 623

Query: 587 MENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKF 636
           M NVPWH +V  F E L      +YEVACEH HS C+L+A   KF+++G+W TWIDY++F
Sbjct: 624 MANVPWHEEVIRFVEEL-VDLLPDYEVACEHEHSNCMLIAH-KKFKINGEWCTWIDYERF 683

BLAST of CSPI02G20010 vs. ExPASy Swiss-Prot
Match: Q08C92 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Danio rerio OX=7955 GN=tyw1 PE=2 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 2.1e-176
Identity = 316/649 (48.69%), Postives = 428/649 (65.95%), Query Frame = 0

Query: 47  KLFFISQTGTSKALAHRLLNVLNSNGLVFDLVDPKDYEPEDLFKE-----TLVLFVASTW 106
           K+F+ SQTGT+K  A  L   + + G+  +++D KD++PED   E      + +F+ +T+
Sbjct: 80  KVFYGSQTGTAKGFAKELAEDVIAQGIQCEVIDMKDFDPEDRLAEECTSKIICVFLVATY 139

Query: 107 EDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRL 166
            DG P   A++   WL E++ DFR G   L   +++VFG+G+ VY   FN V++   K L
Sbjct: 140 TDGQPTESAEWFCKWLEEASTDFRYGKTYLKGMRYAVFGLGNSVYVGHFNTVSKSIDKWL 199

Query: 167 KALGAKEILPIGEGDVD-----GGDIDKCFDDWSRKLLKVMKFDAEGNGVELCSG----- 226
             L A  I+  GEGD +      G +   F  W  K L  ++  A+G   + CSG     
Sbjct: 200 WMLSAARIMTRGEGDCNVVKSRHGSVQADFQVWKGKFLNRLQALAKGE-KKACSGNCKKA 259

Query: 227 ----------IAGDSDAVSVEEDEEE-------DDDLGEED------IVDLEDIAG---- 286
                      A D+ +++ + + EE       D++   ED      ++D+ED+      
Sbjct: 260 SCKNKKKHKEEAEDNHSLAEKNNSEEELMESSSDEESSSEDEKSHGSVIDMEDLGNVMNH 319

Query: 287 --KGPSR-----KSTNIVETNGKLNG-----KKVMVTPVIRASLEKQGYKIIGSHSGVKI 346
             K   R     + +  V+ NG+        ++ M+TP +R SL KQGYK+IGSHSGVK+
Sbjct: 320 MKKAKQRMEEDEEDSQRVKQNGERKSECEEERREMITPALRDSLTKQGYKLIGSHSGVKL 379

Query: 347 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMD 406
            RWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W+MD
Sbjct: 380 RRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMD 439

Query: 407 DPLEIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDE 466
              +I+  A++ H  MI+Q +GVPGV  E+ EEGL+ +HCALSLVGEPIMYPEIN+ +  
Sbjct: 440 PAEKIIQEAMENHRNMIRQFRGVPGVRPERFEEGLTVKHCALSLVGEPIMYPEINSFLKL 499

Query: 467 LHRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSL 526
           LH++ IS+FLVTNAQFP++I+ L PVTQLYVSVDA+TK+SLK IDRPLF DFW+RF+DSL
Sbjct: 500 LHQQNISSFLVTNAQFPEEIRSLVPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSL 559

Query: 527 KALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMEN 586
           +AL EKQQRTVYRLTLVK WN +++ AY++L ++G+PDF+E+KGVTYCG S+ S LTM N
Sbjct: 560 RALGEKQQRTVYRLTLVKAWNVDELKAYADLIALGQPDFIEVKGVTYCGESSASSLTMAN 619

Query: 587 VPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDL 636
           VPWH +V  F + L A    +YE+ACEH HS C+LLA   KF+VDG+W+TWIDY++F +L
Sbjct: 620 VPWHEEVIYFVQQL-ANLLPDYEIACEHEHSNCLLLA-NHKFKVDGEWWTWIDYERFQEL 679

BLAST of CSPI02G20010 vs. ExPASy Swiss-Prot
Match: Q9NV66 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Homo sapiens OX=9606 GN=TYW1 PE=2 SV=2)

HSP 1 Score: 612.8 bits (1579), Expect = 4.3e-174
Identity = 311/650 (47.85%), Postives = 416/650 (64.00%), Query Frame = 0

Query: 47  KLFFISQTGTSKALAHRLLNVLNSNGLVFDLVDPKDYEPED-----LFKETLVLFVASTW 106
           K+F+ SQTGT+K  A  L   + S  L   +++ K+Y+P+D     +  + + +F+ +T+
Sbjct: 80  KIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATY 139

Query: 107 EDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRL 166
            DG P   A++   WL E++ DFR G   L   +++VFG+G+  Y   FN V ++  K L
Sbjct: 140 TDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWL 199

Query: 167 KALGAKEILPIGEGDVD-----GGDIDKCFDDWSRKLLKVMKFDAEGNGVELCSGI---- 226
             LGA  ++  GEGD D      G I+  F  W  K +  ++   +G   + C G     
Sbjct: 200 WMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKG 259

Query: 227 ---------------AGDSDAVSVEEDEEE-------DDDLGEED------IVDLEDIAG 286
                          + + D +   + EEE       +++ G ED      IVD+ED+  
Sbjct: 260 KCESHQHGSEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGK 319

Query: 287 -----KGPSRKSTNIVETNGKLNG--------KKVMVTPVIRASLEKQGYKIIGSHSGVK 346
                K   R+     E +G            ++ M+TP +R +L KQGY++IGSHSGVK
Sbjct: 320 IMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQGYQLIGSHSGVK 379

Query: 347 ICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEM 406
           +CRWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W+M
Sbjct: 380 LCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKM 439

Query: 407 DDPLEIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVD 466
           D P  I+  AI+ H  MIKQ KGVPGV  E+ EEG++ +HCALSLVGEPIMYPEIN  + 
Sbjct: 440 DQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLK 499

Query: 467 ELHRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDS 526
            LH+ +IS+FLVTNAQFP +I+ L+PVTQLYVSVDA+TK+SLK IDRPLF DFW+RF+DS
Sbjct: 500 LLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDS 559

Query: 527 LKALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTME 586
           LKAL  KQQRTVYRLTLVK WN +++ AY+ L S+G PDF+E+KGVTYCG S+ S LTM 
Sbjct: 560 LKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMA 619

Query: 587 NVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHD 636
           +VPWH +V  F   L      EYE+ACEH HS C+L+A   KF++ G+W+TWIDY++F +
Sbjct: 620 HVPWHEEVVQFVHELVDLIP-EYEIACEHEHSNCLLIAHR-KFKIGGEWWTWIDYNRFQE 679

BLAST of CSPI02G20010 vs. ExPASy TrEMBL
Match: A0A0A0LPA4 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis sativus OX=3659 GN=Csa_2G361620 PE=3 SV=1)

HSP 1 Score: 1310.0 bits (3389), Expect = 0.0e+00
Identity = 640/640 (100.00%), Postives = 640/640 (100.00%), Query Frame = 0

Query: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60
           MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE 240
           DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE
Sbjct: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480
           FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ
Sbjct: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480

Query: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD 600
           SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD
Sbjct: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 640

BLAST of CSPI02G20010 vs. ExPASy TrEMBL
Match: A0A5A7VC83 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005790 PE=3 SV=1)

HSP 1 Score: 1285.0 bits (3324), Expect = 0.0e+00
Identity = 627/641 (97.82%), Postives = 635/641 (99.06%), Query Frame = 0

Query: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60
           MS SSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTL SRKPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGET+NAVARDFSKR+KALGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEED-EEEDDDLGEEDIVDL 240
           DIDKCFDDWSRKLLKV+K DAEGNGVELCSGIAGDSDAVSVEE+ EEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCA SLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCAFSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
           TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSS TSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDY+KFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641

BLAST of CSPI02G20010 vs. ExPASy TrEMBL
Match: A0A1S3BAY2 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo OX=3656 GN=LOC103487965 PE=3 SV=1)

HSP 1 Score: 1283.9 bits (3321), Expect = 0.0e+00
Identity = 626/641 (97.66%), Postives = 636/641 (99.22%), Query Frame = 0

Query: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60
           MS SSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTL SRKPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGET+NAVARDFSKR+K+LGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEED-EEEDDDLGEEDIVDL 240
           DIDKCFDDWSRKLLKV+K DAEGNGVELCSGIAGDSDAVSVEE+ EEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
           TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSS TSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDY+KFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           DSKDY+APTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641

BLAST of CSPI02G20010 vs. ExPASy TrEMBL
Match: A0A6J1K9S7 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita maxima OX=3661 GN=LOC111492394 PE=3 SV=1)

HSP 1 Score: 1225.3 bits (3169), Expect = 0.0e+00
Identity = 597/640 (93.28%), Postives = 616/640 (96.25%), Query Frame = 0

Query: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60
           MS SSRSAKL +LALLSASTLYCFYKSRRLRKLKLSLNPT  SR+PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSN L FD+VDPKD+EPEDLFKE LVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKEALVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGETFNAVARDFSKR+KALGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE 240
           DIDKCFDDWSR+LLKV+K DA G G +L SGI  D DA SVEED +ED D GEEDIVDLE
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEED-DEDADPGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480
           FLVTNAQFPDKIK LKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALN+KQQ
Sbjct: 421 FLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480

Query: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWNTEDIDAYSNLFS+G+PDFVEIKGVTYCGSS TSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD 600
           SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDY+KFHDLVASGKPFD
Sbjct: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           SKDYMAPTPSWAVYG+DEGGFDP QSRYRKERHH+PKPTS
Sbjct: 601 SKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPKPTS 639

BLAST of CSPI02G20010 vs. ExPASy TrEMBL
Match: A0A6J1HDZ5 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita moschata OX=3662 GN=LOC111462691 PE=3 SV=1)

HSP 1 Score: 1219.9 bits (3155), Expect = 0.0e+00
Identity = 594/640 (92.81%), Postives = 616/640 (96.25%), Query Frame = 0

Query: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60
           MSLSSRSAKL +LALLSASTLYCFYKSRRLRKLKLSLNPT  SR+PKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSN L FD+VDPKD+EPEDLFKETLVLFVASTWEDGGPPPHAKFLA+WLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGETFNAVARDFSKR+KALGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE 240
           DIDKCFDDWSR+LLKV+K DA G G +L SGI  D DA SVEED +ED D GEEDIVDLE
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEED-DEDADPGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLH KMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480
           FLVTNAQFPDKIK LKPVTQLYVSVDAATK+SLKAIDRPLFGDFWERFIDSLKALN+KQQ
Sbjct: 421 FLVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIDSLKALNDKQQ 480

Query: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWNTEDIDAYSNLFS+G+PDFVEIKGVTYCGSS TSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD 600
           SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFR+DGQWFTWIDY+KFH LVASGKPFD
Sbjct: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRIDGQWFTWIDYEKFHALVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           SKDYMAPTPSWAVYG+DEGGFDP QSRYRKERHH+PKPTS
Sbjct: 601 SKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPKPTS 639

BLAST of CSPI02G20010 vs. NCBI nr
Match: XP_004152698.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis sativus] >KGN62597.1 hypothetical protein Csa_021988 [Cucumis sativus])

HSP 1 Score: 1310.0 bits (3389), Expect = 0.0e+00
Identity = 640/640 (100.00%), Postives = 640/640 (100.00%), Query Frame = 0

Query: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60
           MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE 240
           DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE
Sbjct: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480
           FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ
Sbjct: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480

Query: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD 600
           SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD
Sbjct: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 640

BLAST of CSPI02G20010 vs. NCBI nr
Match: KAA0065238.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis melo var. makuwa])

HSP 1 Score: 1285.0 bits (3324), Expect = 0.0e+00
Identity = 627/641 (97.82%), Postives = 635/641 (99.06%), Query Frame = 0

Query: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60
           MS SSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTL SRKPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGET+NAVARDFSKR+KALGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEED-EEEDDDLGEEDIVDL 240
           DIDKCFDDWSRKLLKV+K DAEGNGVELCSGIAGDSDAVSVEE+ EEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCA SLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCAFSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
           TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSS TSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDY+KFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641

BLAST of CSPI02G20010 vs. NCBI nr
Match: XP_008444711.1 (PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis melo])

HSP 1 Score: 1283.9 bits (3321), Expect = 0.0e+00
Identity = 626/641 (97.66%), Postives = 636/641 (99.22%), Query Frame = 0

Query: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60
           MS SSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTL SRKPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGET+NAVARDFSKR+K+LGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEED-EEEDDDLGEEDIVDL 240
           DIDKCFDDWSRKLLKV+K DAEGNGVELCSGIAGDSDAVSVEE+ EEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
           TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSS TSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDY+KFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           DSKDY+APTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641

BLAST of CSPI02G20010 vs. NCBI nr
Match: XP_038884961.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Benincasa hispida] >XP_038884963.1 S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Benincasa hispida])

HSP 1 Score: 1256.9 bits (3251), Expect = 0.0e+00
Identity = 609/640 (95.16%), Postives = 628/640 (98.12%), Query Frame = 0

Query: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60
           MS SSRSAKLT+LALLSASTLYCFYKSRRLRKLKLSLNPTL SR+PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTLSSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSN LVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLS+CKF+VFGVGSR YGETFNAVAR+FSKR+KALGAKEIL +G+GDVDGG
Sbjct: 121 AEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGKGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE 240
           DID+CFDDWSR+LLKV+K DA GNGV+L SGI GDSDA SVEEDEEED DLGEEDIVDLE
Sbjct: 181 DIDRCFDDWSRQLLKVLKVDAVGNGVDLYSGIGGDSDAESVEEDEEEDADLGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLHTKMIKQMKGVPGVTQEKL+EGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480
           FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALN+KQQ
Sbjct: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480

Query: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSS TSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD 600
           SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDY+KFHDLVASGKPFD
Sbjct: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           SKDYMAPTPSWAVYGSDEGGFDP+QSRYRKERHH+PKPTS
Sbjct: 601 SKDYMAPTPSWAVYGSDEGGFDPNQSRYRKERHHRPKPTS 640

BLAST of CSPI02G20010 vs. NCBI nr
Match: XP_022997485.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita maxima])

HSP 1 Score: 1225.3 bits (3169), Expect = 0.0e+00
Identity = 597/640 (93.28%), Postives = 616/640 (96.25%), Query Frame = 0

Query: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60
           MS SSRSAKL +LALLSASTLYCFYKSRRLRKLKLSLNPT  SR+PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSN L FD+VDPKD+EPEDLFKE LVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKEALVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGETFNAVARDFSKR+KALGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE 240
           DIDKCFDDWSR+LLKV+K DA G G +L SGI  D DA SVEED +ED D GEEDIVDLE
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEED-DEDADPGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480
           FLVTNAQFPDKIK LKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALN+KQQ
Sbjct: 421 FLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480

Query: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWNTEDIDAYSNLFS+G+PDFVEIKGVTYCGSS TSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD 600
           SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDY+KFHDLVASGKPFD
Sbjct: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           SKDYMAPTPSWAVYG+DEGGFDP QSRYRKERHH+PKPTS
Sbjct: 601 SKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPKPTS 639

BLAST of CSPI02G20010 vs. TAIR 10
Match: AT1G75200.1 (flavodoxin family protein / radical SAM domain-containing protein )

HSP 1 Score: 965.7 bits (2495), Expect = 1.9e-281
Identity = 468/640 (73.12%), Postives = 536/640 (83.75%), Query Frame = 0

Query: 4   SSRSAKLTILALLSASTLYCFYKSRRLRKLK-LSLNP--TLPSRKPKLFFISQTGTSKAL 63
           SS   +L  +ALLSA+T YC +K RRL+ LK LSLNP  TL + + K+FFISQTGT+KAL
Sbjct: 5   SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64

Query: 64  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 123
           A RL  +  SN + FD+VDP  YEPEDL KETLVLF+ASTW+ G PP + +FL NWL ES
Sbjct: 65  AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124

Query: 124 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 183
           AEDFRVGSLLLS CKF+VFGVGSR YGE++NAVA++ S R+  LG  E++P+GEGDVD G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184

Query: 184 DIDKCFDDWSRKLLKVMKFDA--EGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVD 243
           ++D+ F DW   +++V+K  +  E NGV    G   D        DEE++D+  +  IVD
Sbjct: 185 ELDRAFQDWCDGVIRVLKGGSAQETNGVSQQIGAVEDDLEYYDSTDEEDEDNDADGGIVD 244

Query: 244 LEDIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKS 303
           LEDIAGK PS+++  +  T  K++GKK MVTPVIRASL KQGYKIIGSHSGVKICRWTKS
Sbjct: 245 LEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICRWTKS 304

Query: 304 QLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIV 363
           QLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MD+P  IV
Sbjct: 305 QLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEPSVIV 364

Query: 364 NSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRI 423
             A+DLH  MIKQMKGVPGVT EKL+EGL+PRHCALSLVGEPIMYPEIN LVDELH RRI
Sbjct: 365 KGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELHGRRI 424

Query: 424 STFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEK 483
           STFLVTNAQFP+KI ++KP+TQLYVSVDAATKESLKAIDRPLF DFWERFIDSLKAL EK
Sbjct: 425 STFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDSLKALQEK 484

Query: 484 QQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSD 543
           QQRTVYRLTLVKGWNTE++DAY NLFSIG+PDF+EIKGVTYCGSS TSKLTMENVPWH+D
Sbjct: 485 QQRTVYRLTLVKGWNTEELDAYFNLFSIGKPDFIEIKGVTYCGSSATSKLTMENVPWHTD 544

Query: 544 VKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKP 603
           VK+FSEAL+ KS GEYEVACEH HSCCVLL +T+KF+VDG+WFTWIDY+KFHDLVASG+P
Sbjct: 545 VKAFSEALSLKSNGEYEVACEHAHSCCVLLGRTEKFKVDGKWFTWIDYEKFHDLVASGEP 604

Query: 604 FDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKP 639
           F S DYMA TPSWAVYG+ EGGFDP Q RY+KER+H PKP
Sbjct: 605 FTSTDYMAQTPSWAVYGAQEGGFDPGQLRYKKERNHPPKP 642