CSPI02G19230 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G19230
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	EF-hand domain-containing protein
Location	Chr2: 17532987 .. 17533286 (+)
RNA-Seq Expression	CSPI02G19230
Synteny	CSPI02G19230

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAATGTCGAGGTCTCAATTGAAGTTGACTAGGGACGAGGTTAGGGAGATCTTAGAAGAACATGATGTGGACGGTGATGGTCGCCTGACTAAGCAAGAGGTGATGCAAGCCCTGAACTCCATGGGTGCTATGATGTCGTTTCAGAAAGCTCACTATGGTGTTGCTTTTGCTGACAAAGATGGAGATGGTGCAGTCGATCTAGAAGGTGGTGAGATTGATAAGCTTCTTGACTATGTCATGAGGTTTCAGGCCCCAAAGGACGTCCCTCAAGATGCCAGCCCAGAAGCCTTGCCATGA

mRNA sequence

ATGGCAATGTCGAGGTCTCAATTGAAGTTGACTAGGGACGAGGTTAGGGAGATCTTAGAAGAACATGATGTGGACGGTGATGGTCGCCTGACTAAGCAAGAGGTGATGCAAGCCCTGAACTCCATGGGTGCTATGATGTCGTTTCAGAAAGCTCACTATGGTGTTGCTTTTGCTGACAAAGATGGAGATGGTGCAGTCGATCTAGAAGGTGGTGAGATTGATAAGCTTCTTGACTATGTCATGAGGTTTCAGGCCCCAAAGGACGTCCCTCAAGATGCCAGCCCAGAAGCCTTGCCATGA

Coding sequence (CDS)

ATGGCAATGTCGAGGTCTCAATTGAAGTTGACTAGGGACGAGGTTAGGGAGATCTTAGAAGAACATGATGTGGACGGTGATGGTCGCCTGACTAAGCAAGAGGTGATGCAAGCCCTGAACTCCATGGGTGCTATGATGTCGTTTCAGAAAGCTCACTATGGTGTTGCTTTTGCTGACAAAGATGGAGATGGTGCAGTCGATCTAGAAGGTGGTGAGATTGATAAGCTTCTTGACTATGTCATGAGGTTTCAGGCCCCAAAGGACGTCCCTCAAGATGCCAGCCCAGAAGCCTTGCCATGA

Protein sequence

MAMSRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMGAMMSFQKAHYGVAFADKDGDGAVDLEGGEIDKLLDYVMRFQAPKDVPQDASPEALP*

Homology

[Close]

BLAST of CSPI02G19230 vs. ExPASy Swiss-Prot
Match: Q0DZP5 (Probable calcium-binding protein CML17 OS=Oryza sativa subsp. japonica OX=39947 GN=CML17 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 3.9e-05
Identity = 25/69 (36.23%), Postives = 43/69 (62.32%), Query Frame = 0


Query: 14 EVREILEEHDVDGDGRLTKQEVMQALNSMGAMMSFQKAHYGVAFADKDGDGAVDLEGGEI 73
          E+R + E  D DGDGR+T++E+ ++L  +G  +  ++    +A  D +GDG VD++  E 
Sbjct: 8  ELRRVFELFDRDGDGRITREELTESLERLGMPVHREELAATIARIDANGDGCVDMD--EF 67

Query: 74 DKLLDYVMR 83
           +L + VMR
Sbjct: 68 TQLYETVMR 74

[Close]

BLAST of CSPI02G19230 vs. ExPASy TrEMBL
Match: A0A0A0LRC3 (EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G359900 PE=4 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 7.5e-44
Identity = 93/99 (93.94%), Postives = 95/99 (95.96%), Query Frame = 0


Query: 1   MAMSRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMGAMMSFQKAHYGVAFADK 60
           MA SRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMG MMSFQKAHYGVAFADK
Sbjct: 1   MATSRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMGVMMSFQKAHYGVAFADK 60

Query: 61  DGDGAVDLEGGEIDKLLDYVMRFQAPKDVPQDASPEALP 100
           DGDGAVDLEGGEID L+DYVMRFQA KDVP+DASPEALP
Sbjct: 61  DGDGAVDLEGGEIDSLVDYVMRFQASKDVPKDASPEALP 99

[Close]

BLAST of CSPI02G19230 vs. ExPASy TrEMBL
Match: A0A0A0LNG3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357860 PE=4 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.3e-32
Identity = 73/87 (83.91%), Postives = 79/87 (90.80%), Query Frame = 0


Query: 1  MAMSRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMGAMMSFQKAHYGVAFADK 60
          MAMSRSQLKLTRDEVREILEEHDVDGDG LTKQEVMQALNSMG+MMSFQKAHYGV+ ADK
Sbjct: 1  MAMSRSQLKLTRDEVREILEEHDVDGDGSLTKQEVMQALNSMGSMMSFQKAHYGVSHADK 60

Query: 61 DGDGAVDLEGGEIDKLLDYVMRFQAPK 88
          DGDG VDL   E++ L+DYVMRFQ P+
Sbjct: 61 DGDGKVDLGEAEMENLIDYVMRFQTPR 87

[Close]

BLAST of CSPI02G19230 vs. ExPASy TrEMBL
Match: A0A5A7V4S6 (Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00280 PE=4 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 8.6e-32
Identity = 74/88 (84.09%), Postives = 80/88 (90.91%), Query Frame = 0


Query: 1  MAMSRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMGAMMSFQKAHYGVAFADK 60
          MAMSRSQLKLTRDEVREILEEHDVDGDG LTKQEVMQALNSMGA+MSFQKAHYGV+ ADK
Sbjct: 1  MAMSRSQLKLTRDEVREILEEHDVDGDGNLTKQEVMQALNSMGALMSFQKAHYGVSHADK 60

Query: 61 DGDGAVDL-EGGEIDKLLDYVMRFQAPK 88
          DGDG VDL    E++KL+DYVMRFQ P+
Sbjct: 61 DGDGKVDLTREDELEKLVDYVMRFQTPR 88

[Close]

BLAST of CSPI02G19230 vs. ExPASy TrEMBL
Match: A0A0A0LP50 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359910 PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 5.4e-18
Identity = 51/90 (56.67%), Postives = 71/90 (78.89%), Query Frame = 0


Query: 1  MAMSRSQLKLTRDE-VREILEEHDVDGDGRLTKQEVMQALNSMGAMMSFQKAHYGVAFAD 60
          M++SRSQL L+RDE +++I EE+DVDGDG LTKQEVMQA+++MG  + FQ   +G++FAD
Sbjct: 1  MSVSRSQLNLSRDEMIKKIFEEYDVDGDGTLTKQEVMQAMDAMGFKILFQMTQFGISFAD 60

Query: 61 KDGDGAVDLEGGEIDKLLDYVMRFQAPKDV 90
          +D DG VD +  E++KL+D V+RFQ  K V
Sbjct: 61 RDDDGKVDQD--ELEKLIDCVIRFQTRKRV 88

[Close]

BLAST of CSPI02G19230 vs. ExPASy TrEMBL
Match: A0A0A0LRC1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 5.6e-15
Identity = 39/84 (46.43%), Postives = 65/84 (77.38%), Query Frame = 0


Query: 1  MAMSRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMGAMMSFQKAHYGVAFADK 60
          M+ + ++L+L+R++++EI  EHD+DGDGRL+  E+++A    G+++ F KAHYG+A AD+
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DGDGAVDLEGGEIDKLLDYVMRFQ 85
          +GDG ++ E  E+DKL+DY  +FQ
Sbjct: 61 NGDGFIEEE--ELDKLVDYAQKFQ 82

[Close]

BLAST of CSPI02G19230 vs. NCBI nr
Match: KGN62521.1 (hypothetical protein Csa_021806 [Cucumis sativus])

HSP 1 Score: 186.0 bits (471), Expect = 1.5e-43
Identity = 93/99 (93.94%), Postives = 95/99 (95.96%), Query Frame = 0


Query: 1   MAMSRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMGAMMSFQKAHYGVAFADK 60
           MA SRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMG MMSFQKAHYGVAFADK
Sbjct: 1   MATSRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMGVMMSFQKAHYGVAFADK 60

Query: 61  DGDGAVDLEGGEIDKLLDYVMRFQAPKDVPQDASPEALP 100
           DGDGAVDLEGGEID L+DYVMRFQA KDVP+DASPEALP
Sbjct: 61  DGDGAVDLEGGEIDSLVDYVMRFQASKDVPKDASPEALP 99

[Close]

BLAST of CSPI02G19230 vs. NCBI nr
Match: KGN62514.1 (hypothetical protein Csa_021819 [Cucumis sativus])

HSP 1 Score: 147.9 bits (372), Expect = 4.7e-32
Identity = 73/87 (83.91%), Postives = 79/87 (90.80%), Query Frame = 0


Query: 1  MAMSRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMGAMMSFQKAHYGVAFADK 60
          MAMSRSQLKLTRDEVREILEEHDVDGDG LTKQEVMQALNSMG+MMSFQKAHYGV+ ADK
Sbjct: 1  MAMSRSQLKLTRDEVREILEEHDVDGDGSLTKQEVMQALNSMGSMMSFQKAHYGVSHADK 60

Query: 61 DGDGAVDLEGGEIDKLLDYVMRFQAPK 88
          DGDG VDL   E++ L+DYVMRFQ P+
Sbjct: 61 DGDGKVDLGEAEMENLIDYVMRFQTPR 87

[Close]

BLAST of CSPI02G19230 vs. NCBI nr
Match: KAA0060895.1 (putative calcium-binding protein CML10 [Cucumis melo var. makuwa])

HSP 1 Score: 146.0 bits (367), Expect = 1.8e-31
Identity = 74/88 (84.09%), Postives = 80/88 (90.91%), Query Frame = 0


Query: 1  MAMSRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMGAMMSFQKAHYGVAFADK 60
          MAMSRSQLKLTRDEVREILEEHDVDGDG LTKQEVMQALNSMGA+MSFQKAHYGV+ ADK
Sbjct: 1  MAMSRSQLKLTRDEVREILEEHDVDGDGNLTKQEVMQALNSMGALMSFQKAHYGVSHADK 60

Query: 61 DGDGAVDL-EGGEIDKLLDYVMRFQAPK 88
          DGDG VDL    E++KL+DYVMRFQ P+
Sbjct: 61 DGDGKVDLTREDELEKLVDYVMRFQTPR 88

[Close]

BLAST of CSPI02G19230 vs. NCBI nr
Match: KAE8652093.1 (hypothetical protein Csa_022146 [Cucumis sativus])

HSP 1 Score: 91.3 bits (225), Expect = 5.2e-15
Identity = 46/82 (56.10%), Postives = 66/82 (80.49%), Query Frame = 0


Query: 1  MAMSRSQLKLTRDE-VREILEEHDVDGDGRLTKQEVMQALNSMGAMMSFQKAHYGVAFAD 60
          M++SRSQL L+RDE +++I EE+DVDGDG LTKQEVMQA+++MG  + FQ   +G++FAD
Sbjct: 1  MSVSRSQLNLSRDEMIKKIFEEYDVDGDGTLTKQEVMQAMDAMGFKILFQMTQFGISFAD 60

Query: 61 KDGDGAVDLEGGEIDKLLDYVM 82
          +D DG VD +  E++KL+D V+
Sbjct: 61 RDDDGKVDQD--ELEKLIDCVI 80

[Close]

BLAST of CSPI02G19230 vs. NCBI nr
Match: XP_004152805.1 (probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 90.1 bits (222), Expect = 1.2e-14
Identity = 39/83 (46.99%), Postives = 64/83 (77.11%), Query Frame = 0


Query: 2   AMSRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMGAMMSFQKAHYGVAFADKD 61
           A+  ++L+L+R++++EI  EHD+DGDGRL+  E+++A    G+++ F KAHYG+A AD++
Sbjct: 83  AIKSTRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADEN 142

Query: 62  GDGAVDLEGGEIDKLLDYVMRFQ 85
           GDG ++ E  E+DKL+DY  +FQ
Sbjct: 143 GDGFIEEE--ELDKLVDYAQKFQ 163

The following BLAST results are available for this feature:

BLAST of CSPI02G19230 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0DZP5	3.9e-05	36.23	Probable calcium-binding protein CML17 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of CSPI02G19230 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRC3	7.5e-44	93.94	EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G359900 PE=...	[more]
A0A0A0LNG3	2.3e-32	83.91	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357860 PE=4 SV=1	[more]
A0A5A7V4S6	8.6e-32	84.09	Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0LP50	5.4e-18	56.67	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359910 PE=4 SV=1	[more]
A0A0A0LRC1	5.6e-15	46.43	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358870 PE=4 SV=1	[more]

BLAST of CSPI02G19230 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN62521.1	1.5e-43	93.94	hypothetical protein Csa_021806 [Cucumis sativus]	[more]
KGN62514.1	4.7e-32	83.91	hypothetical protein Csa_021819 [Cucumis sativus]	[more]
KAA0060895.1	1.8e-31	84.09	putative calcium-binding protein CML10 [Cucumis melo var. makuwa]	[more]
KAE8652093.1	5.2e-15	56.10	hypothetical protein Csa_022146 [Cucumis sativus]	[more]
XP_004152805.1	1.2e-14	46.99	probable calcium-binding protein CML10 [Cucumis sativus]	[more]

BLAST of CSPI02G19230 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 14..42 e-value: 3.1E-4 score: 30.1
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 14..42 e-value: 2.2E-6 score: 26.8
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 10..45 score: 13.592857
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 14..69 e-value: 6.98684E-9 score: 46.0017
None	No IPR available	GENE3D	1.10.238.10		coord: 1..91 e-value: 2.8E-11 score: 45.6
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 23..35
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 11..69

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G19230.1	CSPI02G19230.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding