CSPI02G19190 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G19190
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	calcium-binding protein-like
Location	Chr2: 17520393 .. 17522076 (+)
RNA-Seq Expression	CSPI02G19190
Synteny	CSPI02G19190

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGAAGTATATATTTTTTTACAGAAAAAAAATAATGTTACGTATGTACATGTTAATCTTTCTTCGTTTTCTTTATAAGAAACCAAAAGGCACAAAACACACAAAACACAATTACAAAAACTTTCTATAGCTGAAAGATGAAATGGGAATATGATTCCAGTGAAGAAATTTAGAGATAATACGACTTTTGATAATGCATTATAAATACCATTCGCTTTCACTACTTGATGATACATATCAAACAAAATCTTAAAGCCACGTCTAACTAAAACCCTAAAGCCAACCACAAGAATGGAAGAAAAAAGTCTTTTTGTTGCACTCCAAAAGCCTCTGTCAGAGAAGGAAGGAAACCTTCTGCTCGAGATGCGCGATGCCAACGGCGATGGAGTTCTCGATAAGCAGGAACTGATGGCTGACTTGACACACACTAACAAAAAACCCTCCACAAGCTTACAAGACATGAAACTCACAAGGGAAGAGATCAAAGAAATTTTTGTAGGTTTCGACATCGATGGCGATGGTTTTCTAAGTAAGAATGAGGTAATACAGGCCTTCGGTATGATGGGTTCATGCTCCCCGATTATGAAAGCTCACTACGCCATGGCTTGTGCTGATGAAGATGGCGACGGTCGCATTAGCGAGCCTGAACTTAATAAACTCATTGATTATGTTCAGAGAACCATTAACAGGAGGAGGTTAAATTAATTAGCTATGTGACATATTCATATGTATGTATGTACTGCTACATAATAAATTTAAAGTTTCAAAATAACTTTTGAATTTAATGTGGGTGGAGGATTGAACTCAAATTAAAACTATTAGATTATTGGCAGGTTAAAAATAGCTTCTAGATCCTAAACCTTTCTAAAAGAAATAATTGAGTTATATAAATGAAAATATAGTTTGAGACAATTTATTAGTTTGGTGTACTTTTGAATTTGAAGCAATTTGGTTCCCTATACTTATATTTGTAATAATTTATGAAAGAAGAGTTGTTGATATCCACTTTCCCTTCCTTTCAGCTCCCTCTCTTTTTCCCTTTTGGCACCATCACCACAGTTCACATTAGAGCATTCACCAATCGTTCACCCGTTGGAAGCGATCATGCGCCATTCGTCCTCATTTATCAGCCGTTTTTCATCACCATCAACTTTCGTAGGCCACTTATTGTTGGATAGAAAATTATTGCGAATATCACGTGACTATAAGAATATATTAGTTATAGTAGTTTATCGCTAATAAATTTCATTATATTTATCAAATTTTTGTAGAATATTGTCGTACACTTAAAGTCCCAAAGTTGCTGGGCTGCTGCTTTGTTATGGCCCAAGTGGGAATAAGGGTTTGATCCGATCTGAATTTTGGTGGCAGGTATAAAGGAGGGAGAAGAATAGGAAACATGGAGGAATTGCACATGGGTGATGTAAATTCAATAATCCTAATTTCAATTGTATATCCTTTGATTTGTTGAAATCAGAATTGAAAATCTCTCTCTAAACTCGCTCTTTCCATTGTGATTTTTGGAATAAAATTACGAACAACTCAAACATGGAAATAGAGGAAAGGCCATGATGTACGTGGTTGGGTTAAATGAACCCTAACCATAAGTGGAAGATTTATTCTTTTGAAGGCTACAAGAAGATATATGGTTTATTTATTTCATTTATTTATTTTTTTAATTTTAC

mRNA sequence

CTGAAGTATATATTTTTTTACAGAAAAAAAATAATGTTACGTATGTACATGTTAATCTTTCTTCGTTTTCTTTATAAGAAACCAAAAGGCACAAAACACACAAAACACAATTACAAAAACTTTCTATAGCTGAAAGATGAAATGGGAATATGATTCCAGTGAAGAAATTTAGAGATAATACGACTTTTGATAATGCATTATAAATACCATTCGCTTTCACTACTTGATGATACATATCAAACAAAATCTTAAAGCCACGTCTAACTAAAACCCTAAAGCCAACCACAAGAATGGAAGAAAAAAGTCTTTTTGTTGCACTCCAAAAGCCTCTGTCAGAGAAGGAAGGAAACCTTCTGCTCGAGATGCGCGATGCCAACGGCGATGGAGTTCTCGATAAGCAGGAACTGATGGCTGACTTGACACACACTAACAAAAAACCCTCCACAAGCTTACAAGACATGAAACTCACAAGGGAAGAGATCAAAGAAATTTTTGTAGGTTTCGACATCGATGGCGATGGTTTTCTAAGTAAGAATGAGGTAATACAGGCCTTCGGTATGATGGGTTCATGCTCCCCGATTATGAAAGCTCACTACGCCATGGCTTGTGCTGATGAAGATGGCGACGGTCGCATTAGCGAGCCTGAACTTAATAAACTCATTGATTATGTTCAGAGAACCATTAACAGGAGGAGGTTAAATTAATTAGCTATGTGACATATTCATATGTATAAAGGAGGGAGAAGAATAGGAAACATGGAGGAATTGCACATGGGTGATGTAAATTCAATAATCCTAATTTCAATTGTATATCCTTTGATTTGTTGAAATCAGAATTGAAAATCTCTCTCTAAACTCGCTCTTTCCATTGTGATTTTTGGAATAAAATTACGAACAACTCAAACATGGAAATAGAGGAAAGGCCATGATGTACGTGGTTGGGTTAAATGAACCCTAACCATAAGTGGAAGATTTATTCTTTTGAAGGCTACAAGAAGATATATGGTTTATTTATTTCATTTATTTATTTTTTTAATTTTAC

Coding sequence (CDS)

ATGGAAGAAAAAAGTCTTTTTGTTGCACTCCAAAAGCCTCTGTCAGAGAAGGAAGGAAACCTTCTGCTCGAGATGCGCGATGCCAACGGCGATGGAGTTCTCGATAAGCAGGAACTGATGGCTGACTTGACACACACTAACAAAAAACCCTCCACAAGCTTACAAGACATGAAACTCACAAGGGAAGAGATCAAAGAAATTTTTGTAGGTTTCGACATCGATGGCGATGGTTTTCTAAGTAAGAATGAGGTAATACAGGCCTTCGGTATGATGGGTTCATGCTCCCCGATTATGAAAGCTCACTACGCCATGGCTTGTGCTGATGAAGATGGCGACGGTCGCATTAGCGAGCCTGAACTTAATAAACTCATTGATTATGTTCAGAGAACCATTAACAGGAGGAGGTTAAATTAA

Protein sequence

MEEKSLFVALQKPLSEKEGNLLLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMKLTREEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEPELNKLIDYVQRTINRRRLN*

Homology

[Close]

BLAST of CSPI02G19190 vs. ExPASy Swiss-Prot
Match: Q7XJR9 (Calcium-dependent protein kinase 16 OS=Arabidopsis thaliana OX=3702 GN=CPK16 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 6.7e-08
Identity = 39/126 (30.95%), Postives = 63/126 (50.00%), Query Frame = 0


Query: 1   MEEKSLFVALQKPLSEKEGNL--LLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMK 60
           +EE    +A   P   K+  +  +L+  D+N DG +D  E +A   H N+       D +
Sbjct: 433 LEEMRQALAKDHPWKLKDARVAEILQAIDSNTDGFVDFGEFVAAALHVNQLEE---HDSE 492

Query: 61  LTREEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEP 120
             ++  +  F  FDIDGDGF++  E+    G+ GS  P+++       AD D DG+IS  
Sbjct: 493 KWQQRSRAAFEKFDIDGDGFITAEELRMHTGLKGSIEPLLEE------ADIDNDGKISLQ 549

Query: 121 ELNKLI 125
           E  +L+
Sbjct: 553 EFRRLL 549

[Close]

BLAST of CSPI02G19190 vs. ExPASy Swiss-Prot
Match: Q1PE17 (Calcium-dependent protein kinase 18 OS=Arabidopsis thaliana OX=3702 GN=CPK18 PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 6.7e-08
Identity = 39/126 (30.95%), Postives = 63/126 (50.00%), Query Frame = 0


Query: 1   MEEKSLFVALQKPLSEKEGNL--LLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMK 60
           +EE    +A   P   K+  +  +L+  D+N DG++D  E +    H N+       D +
Sbjct: 396 LEEMRQALAKDVPWKLKDARVAEILQANDSNTDGLVDFTEFVVAALHVNQLEE---HDSE 455

Query: 61  LTREEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEP 120
             ++  +  F  FDIDGDGF++  E+    G+ GS  P+++       AD D DGRIS  
Sbjct: 456 KWQQRSRAAFDKFDIDGDGFITPEELRLQTGLKGSIEPLLEE------ADVDEDGRISIN 512

Query: 121 ELNKLI 125
           E  +L+
Sbjct: 516 EFRRLL 512

[Close]

BLAST of CSPI02G19190 vs. ExPASy Swiss-Prot
Match: Q9SS31 (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.0e-07
Identity = 35/115 (30.43%), Postives = 63/115 (54.78%), Query Frame = 0


Query: 13  PLSEKEGNLLLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMKLTREEIKEIFVGFD 72
           PL+E+E N++L+  D +GDG +  +EL + +   +    ++         E+KE F  FD
Sbjct: 102 PLTEEEINVMLKEVDCDGDGTIRLEELASRVVSLDPARDST---------ELKETFEFFD 161

Query: 73  IDGDGFLSKNEVIQAFGMMGS--CSPIMKAHYAMACADEDGDGRISEPELNKLID 126
            D DG +S +E+++ F  +G   C+ +      +A  DEDGDG +   E ++++D
Sbjct: 162 ADRDGLISADELLRVFSTIGDERCT-LDDCKRMIADVDEDGDGFVCFTEFSRMMD 206

[Close]

BLAST of CSPI02G19190 vs. ExPASy Swiss-Prot
Match: Q0D715 (Calcium-dependent protein kinase 18 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK18 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.7e-06
Identity = 31/103 (30.10%), Postives = 56/103 (54.37%), Query Frame = 0


Query: 22  LLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMKLTREEIKEIFVGFDIDGDGFLSK 81
           ++E  D+N DG++D +E +A   H ++       D +  +   +  F  FD+DGDG+++ 
Sbjct: 400 IVEAIDSNTDGLVDFEEFVAATLHVHQLVE---HDTEKWKSLSQAAFDKFDVDGDGYITS 459

Query: 82  NEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEPELNKLI 125
           +E+    G+ GS  P+++       AD D DG+IS  E  +L+
Sbjct: 460 DELRMQTGLKGSIDPLLEE------ADIDRDGKISLDEFRRLL 493

[Close]

BLAST of CSPI02G19190 vs. ExPASy Swiss-Prot
Match: Q5ZD81 (Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 GN=CML12 PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 6.3e-06
Identity = 40/118 (33.90%), Postives = 61/118 (51.69%), Query Frame = 0


Query: 12  KPLSEKEGNLLLEMRDANGDGVLDKQEL---MADLTHTNKKPSTSLQDMKLTREEIKEIF 71
           +P +  E + L+   DA+G+G ++  EL   +ADL     +PS ++      + E+ E F
Sbjct: 126 RPAAGDEIHALIAAIDADGNGTVEFDELASSLADLILGPCRPSVAVD-----QAELAEAF 185

Query: 72  VGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYA--MACADEDGDGRISEPELNKLI 125
             FD DG+GF+S  E+ ++   MG   PI  A     M  AD DGDG IS  E   ++
Sbjct: 186 RAFDRDGNGFISAAELARSMARMG--HPICYAELTDMMREADTDGDGLISFEEFTAIM 236

[Close]

BLAST of CSPI02G19190 vs. ExPASy TrEMBL
Match: A0A0A0LP46 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358880 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 2.4e-69
Identity = 136/137 (99.27%), Postives = 136/137 (99.27%), Query Frame = 0


Query: 1   MEEKSLFVALQKPLSEKEGNLLLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMKLT 60
           MEEKSLFVALQKPLSEKE NLLLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMKLT
Sbjct: 1   MEEKSLFVALQKPLSEKEANLLLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMKLT 60

Query: 61  REEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEPEL 120
           REEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEPEL
Sbjct: 61  REEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEPEL 120

Query: 121 NKLIDYVQRTINRRRLN 138
           NKLIDYVQRTINRRRLN
Sbjct: 121 NKLIDYVQRTINRRRLN 137

[Close]

BLAST of CSPI02G19190 vs. ExPASy TrEMBL
Match: A0A5A7V530 (Putative calcium-binding protein CML36 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00240 PE=4 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 3.5e-60
Identity = 119/137 (86.86%), Postives = 129/137 (94.16%), Query Frame = 0


Query: 1   MEEKSLFVALQKPLSEKEGNLLLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMKLT 60
           ME+KSLF+ALQKPLSEKE NL+L+MRDANGDG+LDKQELM DLTHTNKKPST+L +MKLT
Sbjct: 1   MEDKSLFLALQKPLSEKEANLVLKMRDANGDGILDKQELMTDLTHTNKKPSTNLPNMKLT 60

Query: 61  REEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEPEL 120
           REEIKEIFVGFDIDGDGFLSKNEV+QAFGMMGS SP++K HYAMACADEDGDG ISE EL
Sbjct: 61  REEIKEIFVGFDIDGDGFLSKNEVMQAFGMMGSYSPLLKTHYAMACADEDGDGLISECEL 120

Query: 121 NKLIDYVQRTINRRRLN 138
           NKLIDYVQRT NRRRLN
Sbjct: 121 NKLIDYVQRTNNRRRLN 137

[Close]

BLAST of CSPI02G19190 vs. ExPASy TrEMBL
Match: A0A1S3BD12 (probable calcium-binding protein CML36 OS=Cucumis melo OX=3656 GN=LOC103488542 PE=4 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 3.9e-59
Identity = 117/137 (85.40%), Postives = 127/137 (92.70%), Query Frame = 0


Query: 1   MEEKSLFVALQKPLSEKEGNLLLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMKLT 60
           ME+KSLF+ALQKPLSEKE NL+ +MRDANGDG+LDKQELM DLTH NKKPST+L +MKLT
Sbjct: 1   MEDKSLFLALQKPLSEKEANLVFKMRDANGDGILDKQELMTDLTHANKKPSTNLPNMKLT 60

Query: 61  REEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEPEL 120
           REEIKEIFVGFDIDGDGFLSKNEV+QAFGMMGS SP++K HYAMACADEDGDG ISE EL
Sbjct: 61  REEIKEIFVGFDIDGDGFLSKNEVMQAFGMMGSYSPLLKTHYAMACADEDGDGLISECEL 120

Query: 121 NKLIDYVQRTINRRRLN 138
           NKLIDYVQRT NRRRLN
Sbjct: 121 NKLIDYVQRTNNRRRLN 137

[Close]

BLAST of CSPI02G19190 vs. ExPASy TrEMBL
Match: A0A6J1KQ84 (probable calcium-binding protein CML15 OS=Cucurbita maxima OX=3661 GN=LOC111496628 PE=4 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 6.3e-25
Identity = 69/127 (54.33%), Postives = 90/127 (70.87%), Query Frame = 0


Query: 4   KSLFVALQKPLSEKEGNLLLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMKLTREE 63
           +SL   LQ+PLSEKE + +L++RD N DGVL+K+ELM DL     +PS     ++LTRE+
Sbjct: 2   ESLQEILQRPLSEKEADCVLKLRDTNKDGVLEKKELMNDL-----RPS----KLQLTREQ 61

Query: 64  IKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEPELNKL 123
           IKEIF+  DIDGDG LS +E+ +AF  MGS     KA + MA AD DGDG ISE EL+KL
Sbjct: 62  IKEIFLEHDIDGDGCLSVSELSKAFNFMGSSLSFHKARHGMALADCDGDGLISEEELDKL 119

Query: 124 IDYVQRT 131
           +DY ++T
Sbjct: 122 VDYFEKT 119

[Close]

BLAST of CSPI02G19190 vs. ExPASy TrEMBL
Match: A0A6J1GIW2 (probable calcium-binding protein CML15 OS=Cucurbita moschata OX=3662 GN=LOC111454618 PE=4 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 6.3e-25
Identity = 69/127 (54.33%), Postives = 89/127 (70.08%), Query Frame = 0


Query: 4   KSLFVALQKPLSEKEGNLLLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMKLTREE 63
           +S+   LQ+PLSEKE + +L++RD N DGVL+K+ELM DL     +PS     ++LTREE
Sbjct: 2   ESIQEILQRPLSEKEADCVLKLRDTNKDGVLEKKELMNDL-----RPS----KLQLTREE 61

Query: 64  IKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEPELNKL 123
           IKEIF+  DIDGDG LS  EV +AF  MGS     KA + +A AD DGDG ISE EL+KL
Sbjct: 62  IKEIFLEHDIDGDGCLSVGEVTKAFNFMGSSLSFHKARHGLALADCDGDGVISEEELDKL 119

Query: 124 IDYVQRT 131
           +DY ++T
Sbjct: 122 VDYFEKT 119

[Close]

BLAST of CSPI02G19190 vs. NCBI nr
Match: XP_004152804.1 (probable calcium-binding protein CML36 [Cucumis sativus] >KGN62517.1 hypothetical protein Csa_022025 [Cucumis sativus])

HSP 1 Score: 271.2 bits (692), Expect = 5.0e-69
Identity = 136/137 (99.27%), Postives = 136/137 (99.27%), Query Frame = 0


Query: 1   MEEKSLFVALQKPLSEKEGNLLLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMKLT 60
           MEEKSLFVALQKPLSEKE NLLLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMKLT
Sbjct: 1   MEEKSLFVALQKPLSEKEANLLLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMKLT 60

Query: 61  REEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEPEL 120
           REEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEPEL
Sbjct: 61  REEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEPEL 120

Query: 121 NKLIDYVQRTINRRRLN 138
           NKLIDYVQRTINRRRLN
Sbjct: 121 NKLIDYVQRTINRRRLN 137

[Close]

BLAST of CSPI02G19190 vs. NCBI nr
Match: KAA0060891.1 (putative calcium-binding protein CML36 [Cucumis melo var. makuwa])

HSP 1 Score: 240.7 bits (613), Expect = 7.3e-60
Identity = 119/137 (86.86%), Postives = 129/137 (94.16%), Query Frame = 0


Query: 1   MEEKSLFVALQKPLSEKEGNLLLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMKLT 60
           ME+KSLF+ALQKPLSEKE NL+L+MRDANGDG+LDKQELM DLTHTNKKPST+L +MKLT
Sbjct: 1   MEDKSLFLALQKPLSEKEANLVLKMRDANGDGILDKQELMTDLTHTNKKPSTNLPNMKLT 60

Query: 61  REEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEPEL 120
           REEIKEIFVGFDIDGDGFLSKNEV+QAFGMMGS SP++K HYAMACADEDGDG ISE EL
Sbjct: 61  REEIKEIFVGFDIDGDGFLSKNEVMQAFGMMGSYSPLLKTHYAMACADEDGDGLISECEL 120

Query: 121 NKLIDYVQRTINRRRLN 138
           NKLIDYVQRT NRRRLN
Sbjct: 121 NKLIDYVQRTNNRRRLN 137

[Close]

BLAST of CSPI02G19190 vs. NCBI nr
Match: XP_008445562.1 (PREDICTED: probable calcium-binding protein CML36 [Cucumis melo])

HSP 1 Score: 237.3 bits (604), Expect = 8.1e-59
Identity = 117/137 (85.40%), Postives = 127/137 (92.70%), Query Frame = 0


Query: 1   MEEKSLFVALQKPLSEKEGNLLLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMKLT 60
           ME+KSLF+ALQKPLSEKE NL+ +MRDANGDG+LDKQELM DLTH NKKPST+L +MKLT
Sbjct: 1   MEDKSLFLALQKPLSEKEANLVFKMRDANGDGILDKQELMTDLTHANKKPSTNLPNMKLT 60

Query: 61  REEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEPEL 120
           REEIKEIFVGFDIDGDGFLSKNEV+QAFGMMGS SP++K HYAMACADEDGDG ISE EL
Sbjct: 61  REEIKEIFVGFDIDGDGFLSKNEVMQAFGMMGSYSPLLKTHYAMACADEDGDGLISECEL 120

Query: 121 NKLIDYVQRTINRRRLN 138
           NKLIDYVQRT NRRRLN
Sbjct: 121 NKLIDYVQRTNNRRRLN 137

[Close]

BLAST of CSPI02G19190 vs. NCBI nr
Match: XP_038885760.1 (probable calcium-binding protein CML36 [Benincasa hispida])

HSP 1 Score: 199.5 bits (506), Expect = 1.9e-47
Identity = 100/131 (76.34%), Postives = 114/131 (87.02%), Query Frame = 0


Query: 7   FVALQKPLSEKEGNLLLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMKLTREEIKE 66
           F+ALQ+PLSEKE NL+LEMRD NGDGVLDKQELMADLT  N KP T+L +M LTREEI+E
Sbjct: 5   FLALQRPLSEKEANLVLEMRDINGDGVLDKQELMADLTQINVKPCTTLPNMHLTREEIRE 64

Query: 67  IFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEPELNKLIDY 126
           IF GFDIDGDGFL+KNE+ QAFGM+GS +P+ KAHYAM  AD+DGDG ISE ELNKLIDY
Sbjct: 65  IFTGFDIDGDGFLNKNELTQAFGMVGSYAPLFKAHYAMVHADDDGDGLISESELNKLIDY 124

Query: 127 VQRTINRRRLN 138
           V++T NRRRLN
Sbjct: 125 VEKT-NRRRLN 134

[Close]

BLAST of CSPI02G19190 vs. NCBI nr
Match: XP_022951882.1 (probable calcium-binding protein CML15 [Cucurbita moschata] >XP_023537876.1 probable calcium-binding protein CML15 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 123.6 bits (309), Expect = 1.3e-24
Identity = 69/127 (54.33%), Postives = 89/127 (70.08%), Query Frame = 0


Query: 4   KSLFVALQKPLSEKEGNLLLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMKLTREE 63
           +S+   LQ+PLSEKE + +L++RD N DGVL+K+ELM DL     +PS     ++LTREE
Sbjct: 2   ESIQEILQRPLSEKEADCVLKLRDTNKDGVLEKKELMNDL-----RPS----KLQLTREE 61

Query: 64  IKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEPELNKL 123
           IKEIF+  DIDGDG LS  EV +AF  MGS     KA + +A AD DGDG ISE EL+KL
Sbjct: 62  IKEIFLEHDIDGDGCLSVGEVTKAFNFMGSSLSFHKARHGLALADCDGDGVISEEELDKL 119

Query: 124 IDYVQRT 131
           +DY ++T
Sbjct: 122 VDYFEKT 119

[Close]

BLAST of CSPI02G19190 vs. TAIR 10
Match: AT2G17890.1 (calcium-dependent protein kinase 16 )

HSP 1 Score: 58.5 bits (140), Expect = 4.8e-09
Identity = 39/126 (30.95%), Postives = 63/126 (50.00%), Query Frame = 0


Query: 1   MEEKSLFVALQKPLSEKEGNL--LLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMK 60
           +EE    +A   P   K+  +  +L+  D+N DG +D  E +A   H N+       D +
Sbjct: 433 LEEMRQALAKDHPWKLKDARVAEILQAIDSNTDGFVDFGEFVAAALHVNQLEE---HDSE 492

Query: 61  LTREEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEP 120
             ++  +  F  FDIDGDGF++  E+    G+ GS  P+++       AD D DG+IS  
Sbjct: 493 KWQQRSRAAFEKFDIDGDGFITAEELRMHTGLKGSIEPLLEE------ADIDNDGKISLQ 549

Query: 121 ELNKLI 125
           E  +L+
Sbjct: 553 EFRRLL 549

[Close]

BLAST of CSPI02G19190 vs. TAIR 10
Match: AT4G36070.1 (calcium-dependent protein kinase 18 )

HSP 1 Score: 58.5 bits (140), Expect = 4.8e-09
Identity = 39/126 (30.95%), Postives = 63/126 (50.00%), Query Frame = 0


Query: 1   MEEKSLFVALQKPLSEKEGNL--LLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMK 60
           +EE    +A   P   K+  +  +L+  D+N DG++D  E +    H N+       D +
Sbjct: 396 LEEMRQALAKDVPWKLKDARVAEILQANDSNTDGLVDFTEFVVAALHVNQLEE---HDSE 455

Query: 61  LTREEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEP 120
             ++  +  F  FDIDGDGF++  E+    G+ GS  P+++       AD D DGRIS  
Sbjct: 456 KWQQRSRAAFDKFDIDGDGFITPEELRLQTGLKGSIEPLLEE------ADVDEDGRISIN 512

Query: 121 ELNKLI 125
           E  +L+
Sbjct: 516 EFRRLL 512

[Close]

BLAST of CSPI02G19190 vs. TAIR 10
Match: AT4G36070.2 (calcium-dependent protein kinase 18 )

HSP 1 Score: 58.5 bits (140), Expect = 4.8e-09
Identity = 39/126 (30.95%), Postives = 63/126 (50.00%), Query Frame = 0


Query: 1   MEEKSLFVALQKPLSEKEGNL--LLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMK 60
           +EE    +A   P   K+  +  +L+  D+N DG++D  E +    H N+       D +
Sbjct: 396 LEEMRQALAKDVPWKLKDARVAEILQANDSNTDGLVDFTEFVVAALHVNQLEE---HDSE 455

Query: 61  LTREEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEP 120
             ++  +  F  FDIDGDGF++  E+    G+ GS  P+++       AD D DGRIS  
Sbjct: 456 KWQQRSRAAFDKFDIDGDGFITPEELRLQTGLKGSIEPLLEE------ADVDEDGRISIN 512

Query: 121 ELNKLI 125
           E  +L+
Sbjct: 516 EFRRLL 512

[Close]

BLAST of CSPI02G19190 vs. TAIR 10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 57.0 bits (136), Expect = 1.4e-08
Identity = 35/115 (30.43%), Postives = 63/115 (54.78%), Query Frame = 0


Query: 13  PLSEKEGNLLLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMKLTREEIKEIFVGFD 72
           PL+E+E N++L+  D +GDG +  +EL + +   +    ++         E+KE F  FD
Sbjct: 102 PLTEEEINVMLKEVDCDGDGTIRLEELASRVVSLDPARDST---------ELKETFEFFD 161

Query: 73  IDGDGFLSKNEVIQAFGMMGS--CSPIMKAHYAMACADEDGDGRISEPELNKLID 126
            D DG +S +E+++ F  +G   C+ +      +A  DEDGDG +   E ++++D
Sbjct: 162 ADRDGLISADELLRVFSTIGDERCT-LDDCKRMIADVDEDGDGFVCFTEFSRMMD 206

[Close]

BLAST of CSPI02G19190 vs. TAIR 10
Match: AT1G18530.1 (EF hand calcium-binding protein family )

HSP 1 Score: 50.8 bits (120), Expect = 1.0e-06
Identity = 40/137 (29.20%), Postives = 67/137 (48.91%), Query Frame = 0


Query: 1   MEEKSLFVALQKPLSEKEGNLLLEMRDANGDGVLDKQELMADLTHTNKKPSTSLQDMKLT 60
           +E  +L  +L    S  + ++LL   D+NG+G ++  EL+  +      P  + +++ + 
Sbjct: 26  LELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEFDELVGTIL-----PDLN-EEVLIN 85

Query: 61  REEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRISEPEL 120
            E++ EIF  FD DG+GF+S  E+  A   MG      +    +  AD +GDG IS  E 
Sbjct: 86  SEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEMIKEADTNGDGVISFGEF 145

Query: 121 NKL-----IDYVQRTIN 133
             +     +DY    IN
Sbjct: 146 ASIMAKSAVDYFGLKIN 156

The following BLAST results are available for this feature:

BLAST of CSPI02G19190 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7XJR9	6.7e-08	30.95	Calcium-dependent protein kinase 16 OS=Arabidopsis thaliana OX=3702 GN=CPK16 PE=...	[more]
Q1PE17	6.7e-08	30.95	Calcium-dependent protein kinase 18 OS=Arabidopsis thaliana OX=3702 GN=CPK18 PE=...	[more]
Q9SS31	2.0e-07	30.43	Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 ...	[more]
Q0D715	1.7e-06	30.10	Calcium-dependent protein kinase 18 OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]
Q5ZD81	6.3e-06	33.90	Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of CSPI02G19190 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LP46	2.4e-69	99.27	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358880 PE=4 SV=1	[more]
A0A5A7V530	3.5e-60	86.86	Putative calcium-binding protein CML36 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BD12	3.9e-59	85.40	probable calcium-binding protein CML36 OS=Cucumis melo OX=3656 GN=LOC103488542 P...	[more]
A0A6J1KQ84	6.3e-25	54.33	probable calcium-binding protein CML15 OS=Cucurbita maxima OX=3661 GN=LOC1114966...	[more]
A0A6J1GIW2	6.3e-25	54.33	probable calcium-binding protein CML15 OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]

BLAST of CSPI02G19190 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152804.1	5.0e-69	99.27	probable calcium-binding protein CML36 [Cucumis sativus] >KGN62517.1 hypothetica...	[more]
KAA0060891.1	7.3e-60	86.86	putative calcium-binding protein CML36 [Cucumis melo var. makuwa]	[more]
XP_008445562.1	8.1e-59	85.40	PREDICTED: probable calcium-binding protein CML36 [Cucumis melo]	[more]
XP_038885760.1	1.9e-47	76.34	probable calcium-binding protein CML36 [Benincasa hispida]	[more]
XP_022951882.1	1.3e-24	54.33	probable calcium-binding protein CML15 [Cucurbita moschata] >XP_023537876.1 prob...	[more]

BLAST of CSPI02G19190 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G17890.1	4.8e-09	30.95	calcium-dependent protein kinase 16	[more]
AT4G36070.1	4.8e-09	30.95	calcium-dependent protein kinase 18	[more]
AT4G36070.2	4.8e-09	30.95	calcium-dependent protein kinase 18	[more]
AT3G10190.1	1.4e-08	30.43	Calcium-binding EF-hand family protein	[more]
AT1G18530.1	1.0e-06	29.20	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 18..46 e-value: 13.0 score: 8.9 coord: 99..127 e-value: 11.0 score: 9.3 coord: 63..91 e-value: 5.1E-4 score: 29.3
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 62..123 e-value: 2.5E-8 score: 34.3
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 27..41 e-value: 0.0015 score: 18.0
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 95..130 score: 9.408546
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 14..49 score: 8.404312
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 59..94 score: 13.481276
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 27..88 e-value: 2.73885E-8 score: 45.6165
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 63..124 e-value: 7.39827E-9 score: 47.1573
None	No IPR available	GENE3D	1.10.238.10		coord: 2..125 e-value: 5.0E-18 score: 67.3
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 59..128
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 21..71
None	No IPR available	PANTHER	PTHR10891:SF861	CALCIUM-BINDING PROTEIN CML45-RELATED	coord: 59..128
None	No IPR available	PANTHER	PTHR10891:SF892	CALCIUM-BINDING PROTEIN CML28-RELATED	coord: 21..71
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 108..120
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 72..84
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 27..39
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 5..124

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G19190.1	CSPI02G19190.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding