CSPI02G18570 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G18570
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionU-box domain-containing protein 4
LocationChr2: 17147191 .. 17149498 (+)
RNA-Seq ExpressionCSPI02G18570
SyntenyCSPI02G18570
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACGCGTTTAAAGACACTATCCTCCCTCGCACGTGCTTCCTTGTGCACGTGTCAGCCCGCTGCCCGAGACCTAGTCCAGGTGTTCCCTCTCTGTTCTTCCTTTATAACCTTCCCCTTCCCCTTCCCCTTTTTTTTCCTTTCTCAAACAAACCCCTCCGAGTTCATTCCTGACTGACTCGGTCTCCTCTTTTTCTTCACTCCAACAACTCAAACCGCCAACTCTATTCAGTATTTATCATTCATTTCTATCCCTTATTGGATAACTGGATTTCCATACTTTCCAATTTTGATATCTTCCCCTTTCTTCTGTTTTCATTTCTTCTTCTTCTTATCTGTCTCCGTTTTTCTCTTTTCCCTTTTATGTGTTATAGAATTCAGAGACCTAAGGTGGGGATTTTGTTCTAATTTCGGAGATGGGGACGGAGAATCCCATGAATTTTACTTACATGGGGAGAACCTTTGATGATCTGAGCAACGGGGATAGTTCTGGGGCTTTTAGTGACTGTAATAGCGATAGATCCGGGGAGTTTCCGACGGCTTCATCGCAGAGTCGGCGGCTTTTGATTGCTTGTGCTTCTGATAACTCCGACGAGTTAATTCGGCATCTGGTTTTGGACCTTGAGTCCTGTTCGATTGAGGAGCAGAAACAGGCGGCTATGGAGATCAGACTTCTAGCCAAGAACAAACCGGAGAATCGATTGAAGATCGCAAAAGCTGGTGCCGTTAGACCGTTGATTTCGCTGATATCATGTACGGATCCTCAGCTTCAAGAGTACGGCGTGACGGCGATTTTGAATCTATCGCTTTGCGACGAGAATAAGGAGTTGATAGCGGCGTCTGGTGCGATTAAGCCACTTGTGAGGGCTCTCATGTCCGGAACTCCAACGGCGAAGGAGAACGCGGCTTGTGCTTTACTGAGGCTTTCGCAAATGGAAGAGAACAAGATAGCAATCGGACGGTCAGGAGCGATTCCACTTCTGGTGAATCTGTTGGAGAACGGCGGGTTTCGGGGAAAGAAGGACGCGTCGACGGCGCTGTATTCGTTGTGTTCGGTTAAAGAGAACAAAATCAGAGCGGTGAAAGCGGGAATCATGAGGCCGCTAGTGGAATTAATGGCGGATTTTGGGTCAAACATGGTGGACAAGTCAGCGTTCGTGCTAAGCGTGTTGGTATCGATGTCGGAAGCCAGGTCGGCTCTGGTGGAAGAAGGCGGAATTCCGGTACTGGTGGAATTAGTGGAGGACGGAACACAGCGGCAAAAGGAAATCGCGGCGGTGATTTTGCTGCAGATTTGCGAGGATAGTGTCCTTTACCGTACAATGGTGGCTCGTGAAGGAGCAATTCCGCCGCTAGTCGCTTTGTCACAATCCGGCACTAATCGCGCCAAACAAAAGGTACGGATTCCCATCTCAAACCTCCGTCTGTTATTCTCAGGCCGACCAATCGGCACAACTGACGTGGCATTTCATTTTCTTTTCCTTATTTAATAAATGAAACGATGGCTTCTTTCTTTATAATTAAAAAATGTGGGATTTGGTTTTTGAGGTGGAAGAGAAGGCTGTTCGGCCACGTCACTCTTTTTCTTGGCTCTATCTTTTATTTAATTTCATTTCCTTTTCTCTAAAGTAAATAAACAAAGACTTTAAAAAAATCTGAGTAATAATTAGTACGAGCGCCTCCCTCTTAACCTTATATTTCATTGCCTAAACTTTAGGTTTAAATTATAATTTAATCCCATCACCAAGAGTTAATGCTTAAAATGTTGGTATTTAAATTTGATTTTGCGTTTTATATACTTATAATTACATGAAATGGAATTGAAATTATTAGAGTACTGATTTTAGAATTGGTTTATTTTGTGGGTGAAAATGAGAACAGGCGGAGAAATTGATAGAGCTTCTACGGCAACCAAGATCCGGCAACTACGCTGCCACCACCTCAGATGTGTCAGTCTAATTTTTGTTCCGGCCACGTGGACGATCCAACCCCCCCACACAACAAGACATCTCAATTACATTACACCCCAAAAAGAGAGAGAGAGATAAAGTGGGGAGAGGTTTTCTTTGTTTTGTAAATATATTTGCGAACTTTTTCTACTTTTCTTTAATGTTTGTTATGATAAATTGATCCTTTTTTTTTCTTTTGTTGAGAACATTGGAAGGCATATTTATACTAATGTGTAAATGGAGAAAAAAAAAAAAAAAAGAGGTGGTCGACCCATTAATGGATTATTCGATCTCTTTTTTTCCTTTGAAATCAGGATAAAGAGATGAGAAAAGAGGGAAAGAGGGAAAGAGAAGTTTTGATA

mRNA sequence

CACGCGTTTAAAGACACTATCCTCCCTCGCACGTGCTTCCTTGTGCACGTGTCAGCCCGCTGCCCGAGACCTAGTCCAGGTGTTCCCTCTCTGTTCTTCCTTTATAACCTTCCCCTTCCCCTTCCCCTTTTTTTTCCTTTCTCAAACAAACCCCTCCGAGTTCATTCCTGACTGACTCGGTCTCCTCTTTTTCTTCACTCCAACAACTCAAACCGCCAACTCTATTCAGTATTTATCATTCATTTCTATCCCTTATTGGATAACTGGATTTCCATACTTTCCAATTTTGATATCTTCCCCTTTCTTCTGTTTTCATTTCTTCTTCTTCTTATCTGTCTCCGTTTTTCTCTTTTCCCTTTTATGTGTTATAGAATTCAGAGACCTAAGGTGGGGATTTTGTTCTAATTTCGGAGATGGGGACGGAGAATCCCATGAATTTTACTTACATGGGGAGAACCTTTGATGATCTGAGCAACGGGGATAGTTCTGGGGCTTTTAGTGACTGTAATAGCGATAGATCCGGGGAGTTTCCGACGGCTTCATCGCAGAGTCGGCGGCTTTTGATTGCTTGTGCTTCTGATAACTCCGACGAGTTAATTCGGCATCTGGTTTTGGACCTTGAGTCCTGTTCGATTGAGGAGCAGAAACAGGCGGCTATGGAGATCAGACTTCTAGCCAAGAACAAACCGGAGAATCGATTGAAGATCGCAAAAGCTGGTGCCGTTAGACCGTTGATTTCGCTGATATCATGTACGGATCCTCAGCTTCAAGAGTACGGCGTGACGGCGATTTTGAATCTATCGCTTTGCGACGAGAATAAGGAGTTGATAGCGGCGTCTGGTGCGATTAAGCCACTTGTGAGGGCTCTCATGTCCGGAACTCCAACGGCGAAGGAGAACGCGGCTTGTGCTTTACTGAGGCTTTCGCAAATGGAAGAGAACAAGATAGCAATCGGACGGTCAGGAGCGATTCCACTTCTGGTGAATCTGTTGGAGAACGGCGGGTTTCGGGGAAAGAAGGACGCGTCGACGGCGCTGTATTCGTTGTGTTCGGTTAAAGAGAACAAAATCAGAGCGGTGAAAGCGGGAATCATGAGGCCGCTAGTGGAATTAATGGCGGATTTTGGGTCAAACATGGTGGACAAGTCAGCGTTCGTGCTAAGCGTGTTGGTATCGATGTCGGAAGCCAGGTCGGCTCTGGTGGAAGAAGGCGGAATTCCGGTACTGGTGGAATTAGTGGAGGACGGAACACAGCGGCAAAAGGAAATCGCGGCGGTGATTTTGCTGCAGATTTGCGAGGATAGTGTCCTTTACCGTACAATGGTGGCTCGTGAAGGAGCAATTCCGCCGCTAGTCGCTTTGTCACAATCCGGCACTAATCGCGCCAAACAAAAGGCGGAGAAATTGATAGAGCTTCTACGGCAACCAAGATCCGGCAACTACGCTGCCACCACCTCAGATGTGTCAGTCTAATTTTTGTTCCGGCCACGTGGACGATCCAACCCCCCCACACAACAAGACATCTCAATTACATTACACCCCAAAAAGAGAGAGAGAGATAAAGTGGGGAGAGGTTTTCTTTGTTTTGTAAATATATTTGCGAACTTTTTCTACTTTTCTTTAATGTTTGTTATGATAAATTGATCCTTTTTTTTTCTTTTGTTGAGAACATTGGAAGGCATATTTATACTAATGTGTAAATGGAGAAAAAAAAAAAAAAAAGAGGTGGTCGACCCATTAATGGATTATTCGATCTCTTTTTTTCCTTTGAAATCAGGATAAAGAGATGAGAAAAGAGGGAAAGAGGGAAAGAGAAGTTTTGATA

Coding sequence (CDS)

ATGGGGACGGAGAATCCCATGAATTTTACTTACATGGGGAGAACCTTTGATGATCTGAGCAACGGGGATAGTTCTGGGGCTTTTAGTGACTGTAATAGCGATAGATCCGGGGAGTTTCCGACGGCTTCATCGCAGAGTCGGCGGCTTTTGATTGCTTGTGCTTCTGATAACTCCGACGAGTTAATTCGGCATCTGGTTTTGGACCTTGAGTCCTGTTCGATTGAGGAGCAGAAACAGGCGGCTATGGAGATCAGACTTCTAGCCAAGAACAAACCGGAGAATCGATTGAAGATCGCAAAAGCTGGTGCCGTTAGACCGTTGATTTCGCTGATATCATGTACGGATCCTCAGCTTCAAGAGTACGGCGTGACGGCGATTTTGAATCTATCGCTTTGCGACGAGAATAAGGAGTTGATAGCGGCGTCTGGTGCGATTAAGCCACTTGTGAGGGCTCTCATGTCCGGAACTCCAACGGCGAAGGAGAACGCGGCTTGTGCTTTACTGAGGCTTTCGCAAATGGAAGAGAACAAGATAGCAATCGGACGGTCAGGAGCGATTCCACTTCTGGTGAATCTGTTGGAGAACGGCGGGTTTCGGGGAAAGAAGGACGCGTCGACGGCGCTGTATTCGTTGTGTTCGGTTAAAGAGAACAAAATCAGAGCGGTGAAAGCGGGAATCATGAGGCCGCTAGTGGAATTAATGGCGGATTTTGGGTCAAACATGGTGGACAAGTCAGCGTTCGTGCTAAGCGTGTTGGTATCGATGTCGGAAGCCAGGTCGGCTCTGGTGGAAGAAGGCGGAATTCCGGTACTGGTGGAATTAGTGGAGGACGGAACACAGCGGCAAAAGGAAATCGCGGCGGTGATTTTGCTGCAGATTTGCGAGGATAGTGTCCTTTACCGTACAATGGTGGCTCGTGAAGGAGCAATTCCGCCGCTAGTCGCTTTGTCACAATCCGGCACTAATCGCGCCAAACAAAAGGCGGAGAAATTGATAGAGCTTCTACGGCAACCAAGATCCGGCAACTACGCTGCCACCACCTCAGATGTGTCAGTCTAA

Protein sequence

MGTENPMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDELIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQEYGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAIGRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSNMVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLYRTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV*
Homology
BLAST of CSPI02G18570 vs. ExPASy Swiss-Prot
Match: O22193 (U-box domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUB4 PE=1 SV=3)

HSP 1 Score: 248.4 bits (633), Expect = 1.2e-64
Identity = 160/345 (46.38%), Postives = 221/345 (64.06%), Query Frame = 0

Query: 6   PMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNS------- 65
           P+  T    T  DLS  D S  F D  +   G+F    S+     I  A  N        
Sbjct: 483 PLAATTSAATRRDLS--DFSPKFMDRRT--RGQFWRRPSERLGSRIVSAPSNETRRDLSE 542

Query: 66  -DELIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQ 125
            +  ++ LV +L+S S++ Q+QA  E+RLLAK+  +NR+ I  +GA+  L+ L+  TD  
Sbjct: 543 VETQVKKLVEELKSSSLDTQRQATAELRLLAKHNMDNRIVIGNSGAIVLLVELLYSTDSA 602

Query: 126 LQEYGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENK 185
            QE  VTA+LNLS+ D NK+ IA +GAI+PL+  L +G+  AKEN+A  L  LS +EENK
Sbjct: 603 TQENAVTALLNLSINDNNKKAIADAGAIEPLIHVLENGSSEAKENSAATLFSLSVIEENK 662

Query: 186 IAIGRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADF 245
           I IG+SGAI  LV+LL NG  RGKKDA+TAL++L   +ENK   V++G +R L++LM D 
Sbjct: 663 IKIGQSGAIGPLVDLLGNGTPRGKKDAATALFNLSIHQENKAMIVQSGAVRYLIDLM-DP 722

Query: 246 GSNMVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDS 305
            + MVDK+  VL+ L ++ E R+A+ +EGGIP+LVE+VE G+ R KE AA  LLQ+  +S
Sbjct: 723 AAGMVDKAVAVLANLATIPEGRNAIGQEGGIPLLVEVVELGSARGKENAAAALLQLSTNS 782

Query: 306 VLYRTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGN 343
             +  MV +EGA+PPLVALSQSGT RA++KA+ L+   R  R GN
Sbjct: 783 GRFCNMVLQEGAVPPLVALSQSGTPRAREKAQALLSYFRNQRHGN 822

BLAST of CSPI02G18570 vs. ExPASy Swiss-Prot
Match: Q5XEZ8 (U-box domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=PUB2 PE=2 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 4.3e-59
Identity = 136/290 (46.90%), Postives = 191/290 (65.86%), Query Frame = 0

Query: 54  ASDNSDELIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISC 113
           +S + +  ++ L+ DL+S S++ Q++A   IR+LA+N  +NR+ IA+  A+  L+SL+  
Sbjct: 415 SSSSIETEVKKLIDDLKSSSLDTQREATARIRILARNSTDNRIVIARCEAIPSLVSLLYS 474

Query: 114 TDPQLQEYGVTAILNLSLCDENKELIAASGAIKPLVRALMSG-TPTAKENAACALLRLSQ 173
           TD ++Q   VT +LNLS+ D NK LIA SGAI PL+  L +G    AK N+A  L  LS 
Sbjct: 475 TDERIQADAVTCLLNLSINDNNKSLIAESGAIVPLIHVLKTGYLEEAKANSAATLFSLSV 534

Query: 174 MEENKIAIGRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVE 233
           +EE K  IG +GAI  LV+LL +G   GKKDA+TAL++L    ENK + ++AG +R LVE
Sbjct: 535 IEEYKTEIGEAGAIEPLVDLLGSGSLSGKKDAATALFNLSIHHENKTKVIEAGAVRYLVE 594

Query: 234 LMADFGSNMVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQ 293
           LM D    MV+K+  VL+ L ++ E + A+ EEGGIPVLVE+VE G+ R KE A   LLQ
Sbjct: 595 LM-DPAFGMVEKAVVVLANLATVREGKIAIGEEGGIPVLVEVVELGSARGKENATAALLQ 654

Query: 294 ICEDSVLYRTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGN 343
           +C  S  +   V REG IPPLVAL++SGT R K+KA+ L++  +  R  N
Sbjct: 655 LCTHSPKFCNNVIREGVIPPLVALTKSGTARGKEKAQNLLKYFKAHRQSN 703

BLAST of CSPI02G18570 vs. ExPASy Swiss-Prot
Match: Q8GWV5 (U-box domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=PUB3 PE=2 SV=2)

HSP 1 Score: 229.6 bits (584), Expect = 5.6e-59
Identity = 139/276 (50.36%), Postives = 189/276 (68.48%), Query Frame = 0

Query: 65  LVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQEYGVT 124
           LV DL+S S + +  AA EIR L  N  ENR+ I + GA+ PL+SL+   +   QE+ VT
Sbjct: 477 LVEDLKSGSNKVKTAAAAEIRHLTINSIENRVHIGRCGAITPLLSLLYSEEKLTQEHAVT 536

Query: 125 AILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAIGRS- 184
           A+LNLS+ + NK +I   GAI+PLV  L +G   AKEN+A +L  LS ++ N+  IG+S 
Sbjct: 537 ALLNLSISELNKAMIVEVGAIEPLVHVLNTGNDRAKENSAASLFSLSVLQVNRERIGQSN 596

Query: 185 GAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSNMVD 244
            AI  LVNLL  G FRGKKDA++AL++L    +NK R V+A  ++ LVEL+ D    MVD
Sbjct: 597 AAIQALVNLLGKGTFRGKKDAASALFNLSITHDNKARIVQAKAVKYLVELL-DPDLEMVD 656

Query: 245 KSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLYRTM 304
           K+  +L+ L ++ E R A+V EGGIP+LVE V+ G+QR KE AA +LLQ+C +S  + T+
Sbjct: 657 KAVALLANLSAVGEGRQAIVREGGIPLLVETVDLGSQRGKENAASVLLQLCLNSPKFCTL 716

Query: 305 VAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPR 340
           V +EGAIPPLVALSQSGT RAK+KA++L+   R  R
Sbjct: 717 VLQEGAIPPLVALSQSGTQRAKEKAQQLLSHFRNQR 751

BLAST of CSPI02G18570 vs. ExPASy Swiss-Prot
Match: Q8VZ40 (U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.1e-57
Identity = 131/285 (45.96%), Postives = 192/285 (67.37%), Query Frame = 0

Query: 54  ASDNSDELIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISC 113
           +SD     +  L+  L + + E+Q+ AA E+RLLAK   +NR+ IA+AGA+  L+ L+S 
Sbjct: 339 SSDCDRTFVLSLLEKLANGTTEQQRAAAGELRLLAKRNVDNRVCIAEAGAIPLLVELLSS 398

Query: 114 TDPQLQEYGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQM 173
            DP+ QE+ VTA+LNLS+ + NK  I  +GAI  +V  L +G+  A+ENAA  L  LS +
Sbjct: 399 PDPRTQEHSVTALLNLSINEGNKGAIVDAGAITDIVEVLKNGSMEARENAAATLFSLSVI 458

Query: 174 EENKIAIGRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVEL 233
           +ENK+AIG +GAI  L++LLE G  RGKKDA+TA+++LC  + NK RAVK GI+ PL  L
Sbjct: 459 DENKVAIGAAGAIQALISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDPLTRL 518

Query: 234 MADFGSNMVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQI 293
           + D G  MVD++  +L++L +  E ++A+ E   IPVLVE++  G+ R +E AA IL  +
Sbjct: 519 LKDAGGGMVDEALAILAILSTNQEGKTAIAEAESIPVLVEIIRTGSPRNRENAAAILWYL 578

Query: 294 CEDSVLYRTMVARE-GAIPPLVALSQSGTNRAKQKAEKLIELLRQ 338
           C  ++  R  VARE GA   L  L+++GT+RAK+KA  L+EL++Q
Sbjct: 579 CIGNI-ERLNVAREVGADVALKELTENGTDRAKRKAASLLELIQQ 622

BLAST of CSPI02G18570 vs. ExPASy Swiss-Prot
Match: Q8GUG9 (U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2 SV=2)

HSP 1 Score: 224.6 bits (571), Expect = 1.8e-57
Identity = 129/278 (46.40%), Postives = 188/278 (67.63%), Query Frame = 0

Query: 61  LIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQE 120
           +IR LV  L S S E+++ A  EIR L+K   +NR+ IA+AGA+  L++L++  D   QE
Sbjct: 332 VIRALVQRLSSRSTEDRRNAVSEIRSLSKRSTDNRILIAEAGAIPVLVNLLTSEDVATQE 391

Query: 121 YGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180
             +T +LNLS+ + NKELI  +GA+  +V+ L +GT  A+ENAA  L  LS  +ENKI I
Sbjct: 392 NAITCVLNLSIYENNKELIMFAGAVTSIVQVLRAGTMEARENAAATLFSLSLADENKIII 451

Query: 181 GRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240
           G SGAIP LV+LLENG  RGKKDA+TAL++LC    NK RAV+AGI+  LV++++D   +
Sbjct: 452 GGSGAIPALVDLLENGTPRGKKDAATALFNLCIYHGNKGRAVRAGIVTALVKMLSDSTRH 511

Query: 241 -MVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVL 300
            MVD++  +LSVL +  +A+SA+V+   +P L+ +++    R +E AA ILL +C+    
Sbjct: 512 RMVDEALTILSVLANNQDAKSAIVKANTLPALIGILQTDQTRNRENAAAILLSLCKRDTE 571

Query: 301 YRTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQ 338
               + R GA+ PL+ LS++GT R K+KA  L+ELLR+
Sbjct: 572 KLITIGRLGAVVPLMDLSKNGTERGKRKAISLLELLRK 609

BLAST of CSPI02G18570 vs. ExPASy TrEMBL
Match: A0A0A0LR48 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G354770 PE=4 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 2.8e-186
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0

Query: 1   MGTENPMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDE 60
           MGTENPMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDE
Sbjct: 1   MGTENPMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDE 60

Query: 61  LIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQE 120
           LIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQE
Sbjct: 61  LIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQE 120

Query: 121 YGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180
           YGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI
Sbjct: 121 YGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180

Query: 181 GRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240
           GRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN
Sbjct: 181 GRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240

Query: 241 MVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLY 300
           MVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLY
Sbjct: 241 MVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLY 300

Query: 301 RTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV 353
           RTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV
Sbjct: 301 RTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV 352

BLAST of CSPI02G18570 vs. ExPASy TrEMBL
Match: A0A1S3BBF2 (U-box domain-containing protein 4 OS=Cucumis melo OX=3656 GN=LOC103487841 PE=4 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 5.1e-180
Identity = 341/346 (98.55%), Postives = 344/346 (99.42%), Query Frame = 0

Query: 7   MNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDELIRHLV 66
           MNFTYMGR+FDDLSNGDSSGAFSDCNSDRSGEFPT SSQSRRLLIACASDNSDELIRHLV
Sbjct: 1   MNFTYMGRSFDDLSNGDSSGAFSDCNSDRSGEFPTGSSQSRRLLIACASDNSDELIRHLV 60

Query: 67  LDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQEYGVTAI 126
           LDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQEYGVTAI
Sbjct: 61  LDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQEYGVTAI 120

Query: 127 LNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAIGRSGAI 186
           LNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAIGRSGAI
Sbjct: 121 LNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAIGRSGAI 180

Query: 187 PLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSNMVDKSA 246
           PLLVNLLE+GGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSNMVDKSA
Sbjct: 181 PLLVNLLESGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSNMVDKSA 240

Query: 247 FVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLYRTMVAR 306
           FVLSVLVSM EAR+ALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLYRTMVAR
Sbjct: 241 FVLSVLVSMPEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLYRTMVAR 300

Query: 307 EGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV 353
           EGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV
Sbjct: 301 EGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV 346

BLAST of CSPI02G18570 vs. ExPASy TrEMBL
Match: A0A6J1GGH6 (U-box domain-containing protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC111453995 PE=4 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 4.1e-177
Identity = 333/352 (94.60%), Postives = 345/352 (98.01%), Query Frame = 0

Query: 1   MGTENPMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDE 60
           MGT++P NFTYMGRTFDDL+NG SSGAFSDCNSDRSGEFPTA+SQ RRLLIACASDNSD+
Sbjct: 1   MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60

Query: 61  LIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQE 120
           LIR LV+DLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISC+DPQLQE
Sbjct: 61  LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE 120

Query: 121 YGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180
           YGVTAILNLSLCDENKE IAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI
Sbjct: 121 YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180

Query: 181 GRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240
           GR GAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN
Sbjct: 181 GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240

Query: 241 MVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLY 300
           MVDKSAFVLSVLVS+SEAR+ALVEEGGIPVLVELVEDGTQRQKEIAAVILLQ+CEDSVLY
Sbjct: 241 MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY 300

Query: 301 RTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV 353
           RTMVAREGAIPPL+AL+QSGTNRAKQKAEKLIELLRQPRSGN AATTSDVSV
Sbjct: 301 RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGNCAATTSDVSV 352

BLAST of CSPI02G18570 vs. ExPASy TrEMBL
Match: A0A6J1KKD4 (U-box domain-containing protein 4-like OS=Cucurbita maxima OX=3661 GN=LOC111496516 PE=4 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 1.5e-176
Identity = 332/352 (94.32%), Postives = 345/352 (98.01%), Query Frame = 0

Query: 1   MGTENPMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDE 60
           MGTE+P+NFTYMGRTFDDL+NG SSGAFSDCNSDRSGEFPTA+SQ RRLLIACASDNSD+
Sbjct: 1   MGTESPVNFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60

Query: 61  LIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQE 120
           LIR LV+DL+SCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISC+DPQLQE
Sbjct: 61  LIRQLVMDLQSCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE 120

Query: 121 YGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180
           YGVTAILNLSLCDENKE IAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI
Sbjct: 121 YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180

Query: 181 GRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240
           GR GAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN
Sbjct: 181 GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240

Query: 241 MVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLY 300
           MVDKSAFVLSVLVS+SEAR+ALVEEGGIPVLVELVEDGTQRQKEIAAVILLQ+CEDSVLY
Sbjct: 241 MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY 300

Query: 301 RTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV 353
           RTMVAREGAIPPL+AL+QSGTNRAKQKAEKLIELLR PRSGN AATTSDVSV
Sbjct: 301 RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRLPRSGNCAATTSDVSV 352

BLAST of CSPI02G18570 vs. ExPASy TrEMBL
Match: A0A6J1HEZ8 (U-box domain-containing protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC111462862 PE=4 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 5.9e-176
Identity = 334/352 (94.89%), Postives = 346/352 (98.30%), Query Frame = 0

Query: 1   MGTENPMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDE 60
           MGTENP+NF+YMGR+FDDL+NGDSSGAFSDCNSDRSGEFP ASSQSRRLLI+CASDNSDE
Sbjct: 1   MGTENPVNFSYMGRSFDDLTNGDSSGAFSDCNSDRSGEFPMASSQSRRLLISCASDNSDE 60

Query: 61  LIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQE 120
           LI+ LVLDLESCSI+EQKQAAMEIRLLAKNKPENRL+IAKAGAVRPLISLISCTDPQLQE
Sbjct: 61  LIQQLVLDLESCSIDEQKQAAMEIRLLAKNKPENRLRIAKAGAVRPLISLISCTDPQLQE 120

Query: 121 YGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180
           YGVTAILNLSL DENKELIAASGAIKPLVRALMSGT TAKENAACALLRLSQMEENKIAI
Sbjct: 121 YGVTAILNLSLNDENKELIAASGAIKPLVRALMSGTSTAKENAACALLRLSQMEENKIAI 180

Query: 181 GRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240
           GRSGAIPLLVNLL NGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN
Sbjct: 181 GRSGAIPLLVNLLGNGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240

Query: 241 MVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLY 300
           MVDKSAFVLSVLVSMSEAR+ALV+EGGIPVLVELVEDGTQRQKEIAAVILLQICE+SVLY
Sbjct: 241 MVDKSAFVLSVLVSMSEARTALVDEGGIPVLVELVEDGTQRQKEIAAVILLQICEESVLY 300

Query: 301 RTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV 353
           RTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTS+V V
Sbjct: 301 RTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSNVCV 352

BLAST of CSPI02G18570 vs. NCBI nr
Match: XP_004142891.1 (U-box domain-containing protein 4 [Cucumis sativus] >KGN62451.1 hypothetical protein Csa_018696 [Cucumis sativus])

HSP 1 Score: 661.0 bits (1704), Expect = 5.8e-186
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0

Query: 1   MGTENPMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDE 60
           MGTENPMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDE
Sbjct: 1   MGTENPMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDE 60

Query: 61  LIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQE 120
           LIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQE
Sbjct: 61  LIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQE 120

Query: 121 YGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180
           YGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI
Sbjct: 121 YGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180

Query: 181 GRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240
           GRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN
Sbjct: 181 GRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240

Query: 241 MVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLY 300
           MVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLY
Sbjct: 241 MVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLY 300

Query: 301 RTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV 353
           RTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV
Sbjct: 301 RTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV 352

BLAST of CSPI02G18570 vs. NCBI nr
Match: XP_038886361.1 (U-box domain-containing protein 4-like [Benincasa hispida])

HSP 1 Score: 653.3 bits (1684), Expect = 1.2e-183
Identity = 348/352 (98.86%), Postives = 350/352 (99.43%), Query Frame = 0

Query: 1   MGTENPMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDE 60
           MGTENPMNFTYMGR+FDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDE
Sbjct: 1   MGTENPMNFTYMGRSFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDE 60

Query: 61  LIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQE 120
           LIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQE
Sbjct: 61  LIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQE 120

Query: 121 YGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180
           YGVTAILNLSLCDENKELIAASGAIKPLVRALMSGT TAKENAACALLRLSQMEENKIAI
Sbjct: 121 YGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTSTAKENAACALLRLSQMEENKIAI 180

Query: 181 GRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240
           GRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN
Sbjct: 181 GRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240

Query: 241 MVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLY 300
           MVDKSAFVLSVLVSM EAR+ALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLY
Sbjct: 241 MVDKSAFVLSVLVSMPEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLY 300

Query: 301 RTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV 353
           RTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV
Sbjct: 301 RTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV 352

BLAST of CSPI02G18570 vs. NCBI nr
Match: XP_008444561.1 (PREDICTED: U-box domain-containing protein 4 [Cucumis melo])

HSP 1 Score: 640.2 bits (1650), Expect = 1.1e-179
Identity = 341/346 (98.55%), Postives = 344/346 (99.42%), Query Frame = 0

Query: 7   MNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDELIRHLV 66
           MNFTYMGR+FDDLSNGDSSGAFSDCNSDRSGEFPT SSQSRRLLIACASDNSDELIRHLV
Sbjct: 1   MNFTYMGRSFDDLSNGDSSGAFSDCNSDRSGEFPTGSSQSRRLLIACASDNSDELIRHLV 60

Query: 67  LDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQEYGVTAI 126
           LDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQEYGVTAI
Sbjct: 61  LDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQEYGVTAI 120

Query: 127 LNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAIGRSGAI 186
           LNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAIGRSGAI
Sbjct: 121 LNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAIGRSGAI 180

Query: 187 PLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSNMVDKSA 246
           PLLVNLLE+GGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSNMVDKSA
Sbjct: 181 PLLVNLLESGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSNMVDKSA 240

Query: 247 FVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLYRTMVAR 306
           FVLSVLVSM EAR+ALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLYRTMVAR
Sbjct: 241 FVLSVLVSMPEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLYRTMVAR 300

Query: 307 EGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV 353
           EGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV
Sbjct: 301 EGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV 346

BLAST of CSPI02G18570 vs. NCBI nr
Match: XP_022951022.1 (U-box domain-containing protein 4-like [Cucurbita moschata] >KAG7020606.1 U-box domain-containing protein 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 630.6 bits (1625), Expect = 8.4e-177
Identity = 333/352 (94.60%), Postives = 345/352 (98.01%), Query Frame = 0

Query: 1   MGTENPMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDE 60
           MGT++P NFTYMGRTFDDL+NG SSGAFSDCNSDRSGEFPTA+SQ RRLLIACASDNSD+
Sbjct: 1   MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60

Query: 61  LIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQE 120
           LIR LV+DLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISC+DPQLQE
Sbjct: 61  LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE 120

Query: 121 YGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180
           YGVTAILNLSLCDENKE IAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI
Sbjct: 121 YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180

Query: 181 GRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240
           GR GAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN
Sbjct: 181 GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240

Query: 241 MVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLY 300
           MVDKSAFVLSVLVS+SEAR+ALVEEGGIPVLVELVEDGTQRQKEIAAVILLQ+CEDSVLY
Sbjct: 241 MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY 300

Query: 301 RTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV 353
           RTMVAREGAIPPL+AL+QSGTNRAKQKAEKLIELLRQPRSGN AATTSDVSV
Sbjct: 301 RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGNCAATTSDVSV 352

BLAST of CSPI02G18570 vs. NCBI nr
Match: XP_023536752.1 (U-box domain-containing protein 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 629.4 bits (1622), Expect = 1.9e-176
Identity = 332/352 (94.32%), Postives = 345/352 (98.01%), Query Frame = 0

Query: 1   MGTENPMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDE 60
           MGT++P NFTYMGRTFDDL+NG SSGAFSDCNSDRSGEFPTA+SQ RRLLIACASDNSD+
Sbjct: 1   MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60

Query: 61  LIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQE 120
           LIR LV+DLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISC+DPQLQE
Sbjct: 61  LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE 120

Query: 121 YGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180
           YGVTAILNLSLCDENKE IAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI
Sbjct: 121 YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180

Query: 181 GRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240
           GR GAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN
Sbjct: 181 GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240

Query: 241 MVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLY 300
           MVDKSAFVLSVLVS+SEAR+ALVEEGGIPVLVELVEDGTQRQKEIAAVILLQ+CEDSVLY
Sbjct: 241 MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY 300

Query: 301 RTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV 353
           RTMVAREGAIPPL+AL+QSGTNRA+QKAEKLIELLRQPRSGN AATTSDVSV
Sbjct: 301 RTMVAREGAIPPLIALTQSGTNRARQKAEKLIELLRQPRSGNCAATTSDVSV 352

BLAST of CSPI02G18570 vs. TAIR 10
Match: AT3G01400.1 (ARM repeat superfamily protein )

HSP 1 Score: 523.5 bits (1347), Expect = 1.3e-148
Identity = 277/341 (81.23%), Postives = 311/341 (91.20%), Query Frame = 0

Query: 1   MGTENPMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDE 60
           M    P +FTYMGR F DLS  D S AFSDCNSDRSGEFPTASS+SRRLL++CAS+NSD+
Sbjct: 3   MENHRPGSFTYMGRKFSDLSLNDDSSAFSDCNSDRSGEFPTASSESRRLLLSCASENSDD 62

Query: 61  LIRHLVLDLESC-SIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQ 120
           LI HLV  L+S  SI+EQKQAAMEIRLL+KNKPENR+KIAKAGA++PLISLIS +D QLQ
Sbjct: 63  LINHLVSHLDSSYSIDEQKQAAMEIRLLSKNKPENRIKIAKAGAIKPLISLISSSDLQLQ 122

Query: 121 EYGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIA 180
           EYGVTAILNLSLCDENKE IA+SGAIKPLVRAL  GTPTAKENAACALLRLSQ+EENK+A
Sbjct: 123 EYGVTAILNLSLCDENKESIASSGAIKPLVRALKMGTPTAKENAACALLRLSQIEENKVA 182

Query: 181 IGRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGS 240
           IGRSGAIPLLVNLLE GGFR KKDASTALYSLCS KENKIRAV++GIM+PLVELMADFGS
Sbjct: 183 IGRSGAIPLLVNLLETGGFRAKKDASTALYSLCSAKENKIRAVQSGIMKPLVELMADFGS 242

Query: 241 NMVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVL 300
           NMVDKSAFV+S+L+S+ E++ A+VEEGG+PVLVE+VE GTQRQKE+A  ILLQ+CE+SV+
Sbjct: 243 NMVDKSAFVMSLLMSVPESKPAIVEEGGVPVLVEIVEVGTQRQKEMAVSILLQLCEESVV 302

Query: 301 YRTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRS 341
           YRTMVAREGAIPPLVALSQ+GT+RAKQKAE LIELLRQPRS
Sbjct: 303 YRTMVAREGAIPPLVALSQAGTSRAKQKAEALIELLRQPRS 343

BLAST of CSPI02G18570 vs. TAIR 10
Match: AT5G58680.1 (ARM repeat superfamily protein )

HSP 1 Score: 464.9 bits (1195), Expect = 5.7e-131
Identity = 249/339 (73.45%), Postives = 292/339 (86.14%), Query Frame = 0

Query: 5   NPMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQS--RRLLIACASDNSDELI 64
           N  +FTYM  +F D+S    S AFSDCNSD S EFPT +S+S  R+L ++CA DNSD++I
Sbjct: 3   NHNSFTYMDHSFSDISLNFDSSAFSDCNSDISCEFPTTTSESRQRKLFLSCAVDNSDDVI 62

Query: 65  RHLVLDLE-SCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQEY 124
           R+L+  LE S SIEEQKQAAMEIRLL+KNKPENR+K+AKAGA++PL+SLIS +D QLQEY
Sbjct: 63  RNLITHLESSSSIEEQKQAAMEIRLLSKNKPENRIKLAKAGAIKPLVSLISSSDLQLQEY 122

Query: 125 GVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAIG 184
           GVTA+LNLSLCDENKE+I +SGA+KPLV AL  GTPT KENAACALLRLSQ+EENKI IG
Sbjct: 123 GVTAVLNLSLCDENKEMIVSSGAVKPLVNALRLGTPTTKENAACALLRLSQVEENKITIG 182

Query: 185 RSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSNM 244
           RSGAIPLLVNLLENGGFR KKDASTALYSLCS  ENK RAV++GIM+PLVELM DF S+M
Sbjct: 183 RSGAIPLLVNLLENGGFRAKKDASTALYSLCSTNENKTRAVESGIMKPLVELMIDFESDM 242

Query: 245 VDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLYR 304
           VDKSAFV+++L+S  E++ A+VEEGG+PVLVE+VE GTQRQKEI+  ILLQ+CE+SV+YR
Sbjct: 243 VDKSAFVMNLLMSAPESKPAVVEEGGVPVLVEIVEAGTQRQKEISVSILLQLCEESVVYR 302

Query: 305 TMVAREGAIPPLVALSQSGTNR-AKQKAEKLIELLRQPR 340
           TMVAREGA+PPLVALSQ   +R AK KAE LIELLRQPR
Sbjct: 303 TMVAREGAVPPLVALSQGSASRGAKVKAEALIELLRQPR 341

BLAST of CSPI02G18570 vs. TAIR 10
Match: AT4G16490.1 (ARM repeat superfamily protein )

HSP 1 Score: 282.3 bits (721), Expect = 5.2e-76
Identity = 157/293 (53.58%), Postives = 213/293 (72.70%), Query Frame = 0

Query: 50  LIACASDNSDELIRHLVLD-LESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLI 109
           +I C S    +    L +D L S S+  ++ AA ++RLLAKN+ +NR+ I ++GA++ LI
Sbjct: 172 IIECISPEDLQPTVKLCIDGLRSSSVAIKRSAAAKLRLLAKNRADNRVLIGESGAIQALI 231

Query: 110 SLISCTDPQLQEYGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALL 169
            L+ C DP  QE+ VTA+LNLSL D+NK +IAA GAIK LV  L +GT T+K+NAACALL
Sbjct: 232 PLLRCNDPWTQEHAVTALLNLSLHDQNKAVIAAGGAIKSLVWVLKTGTETSKQNAACALL 291

Query: 170 RLSQMEENKIAIGRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMR 229
            L+ +EENK +IG  GAIP LV+LL NG  RGKKDA T LY LC++++NK RAV AG ++
Sbjct: 292 SLALLEENKGSIGACGAIPPLVSLLLNGSCRGKKDALTTLYKLCTLQQNKERAVTAGAVK 351

Query: 230 PLVELMADFGSNMVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAV 289
           PLV+L+A+ G+ M +K+  VLS L ++ + + A+VEEGGI  LVE +EDG+ + KE A +
Sbjct: 352 PLVDLVAEEGTGMAEKAMVVLSSLAAIDDGKEAIVEEGGIAALVEAIEDGSVKGKEFAIL 411

Query: 290 ILLQICEDSVLYRTMVAREGAIPPLVALSQSG--TNRAKQKAEKLIELLRQPR 340
            LLQ+C DSV  R ++ REGAIPPLV LSQSG  + RAK+KAE+L+  LR+PR
Sbjct: 412 TLLQLCSDSVRNRGLLVREGAIPPLVGLSQSGSVSVRAKRKAERLLGYLREPR 464

BLAST of CSPI02G18570 vs. TAIR 10
Match: AT2G23140.1 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 248.4 bits (633), Expect = 8.3e-66
Identity = 160/345 (46.38%), Postives = 221/345 (64.06%), Query Frame = 0

Query: 6   PMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNS------- 65
           P+  T    T  DLS  D S  F D  +   G+F    S+     I  A  N        
Sbjct: 486 PLAATTSAATRRDLS--DFSPKFMDRRT--RGQFWRRPSERLGSRIVSAPSNETRRDLSE 545

Query: 66  -DELIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQ 125
            +  ++ LV +L+S S++ Q+QA  E+RLLAK+  +NR+ I  +GA+  L+ L+  TD  
Sbjct: 546 VETQVKKLVEELKSSSLDTQRQATAELRLLAKHNMDNRIVIGNSGAIVLLVELLYSTDSA 605

Query: 126 LQEYGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENK 185
            QE  VTA+LNLS+ D NK+ IA +GAI+PL+  L +G+  AKEN+A  L  LS +EENK
Sbjct: 606 TQENAVTALLNLSINDNNKKAIADAGAIEPLIHVLENGSSEAKENSAATLFSLSVIEENK 665

Query: 186 IAIGRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADF 245
           I IG+SGAI  LV+LL NG  RGKKDA+TAL++L   +ENK   V++G +R L++LM D 
Sbjct: 666 IKIGQSGAIGPLVDLLGNGTPRGKKDAATALFNLSIHQENKAMIVQSGAVRYLIDLM-DP 725

Query: 246 GSNMVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDS 305
            + MVDK+  VL+ L ++ E R+A+ +EGGIP+LVE+VE G+ R KE AA  LLQ+  +S
Sbjct: 726 AAGMVDKAVAVLANLATIPEGRNAIGQEGGIPLLVEVVELGSARGKENAAAALLQLSTNS 785

Query: 306 VLYRTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGN 343
             +  MV +EGA+PPLVALSQSGT RA++KA+ L+   R  R GN
Sbjct: 786 GRFCNMVLQEGAVPPLVALSQSGTPRAREKAQALLSYFRNQRHGN 825

BLAST of CSPI02G18570 vs. TAIR 10
Match: AT2G23140.2 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 248.4 bits (633), Expect = 8.3e-66
Identity = 160/345 (46.38%), Postives = 221/345 (64.06%), Query Frame = 0

Query: 6   PMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNS------- 65
           P+  T    T  DLS  D S  F D  +   G+F    S+     I  A  N        
Sbjct: 483 PLAATTSAATRRDLS--DFSPKFMDRRT--RGQFWRRPSERLGSRIVSAPSNETRRDLSE 542

Query: 66  -DELIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQ 125
            +  ++ LV +L+S S++ Q+QA  E+RLLAK+  +NR+ I  +GA+  L+ L+  TD  
Sbjct: 543 VETQVKKLVEELKSSSLDTQRQATAELRLLAKHNMDNRIVIGNSGAIVLLVELLYSTDSA 602

Query: 126 LQEYGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENK 185
            QE  VTA+LNLS+ D NK+ IA +GAI+PL+  L +G+  AKEN+A  L  LS +EENK
Sbjct: 603 TQENAVTALLNLSINDNNKKAIADAGAIEPLIHVLENGSSEAKENSAATLFSLSVIEENK 662

Query: 186 IAIGRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADF 245
           I IG+SGAI  LV+LL NG  RGKKDA+TAL++L   +ENK   V++G +R L++LM D 
Sbjct: 663 IKIGQSGAIGPLVDLLGNGTPRGKKDAATALFNLSIHQENKAMIVQSGAVRYLIDLM-DP 722

Query: 246 GSNMVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDS 305
            + MVDK+  VL+ L ++ E R+A+ +EGGIP+LVE+VE G+ R KE AA  LLQ+  +S
Sbjct: 723 AAGMVDKAVAVLANLATIPEGRNAIGQEGGIPLLVEVVELGSARGKENAAAALLQLSTNS 782

Query: 306 VLYRTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGN 343
             +  MV +EGA+PPLVALSQSGT RA++KA+ L+   R  R GN
Sbjct: 783 GRFCNMVLQEGAVPPLVALSQSGTPRAREKAQALLSYFRNQRHGN 822

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O221931.2e-6446.38U-box domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUB4 PE=1 S... [more]
Q5XEZ84.3e-5946.90U-box domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=PUB2 PE=2 S... [more]
Q8GWV55.6e-5950.36U-box domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=PUB3 PE=2 S... [more]
Q8VZ401.1e-5745.96U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1... [more]
Q8GUG91.8e-5746.40U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2... [more]
Match NameE-valueIdentityDescription
A0A0A0LR482.8e-186100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G354770 PE=4 SV=1[more]
A0A1S3BBF25.1e-18098.55U-box domain-containing protein 4 OS=Cucumis melo OX=3656 GN=LOC103487841 PE=4 S... [more]
A0A6J1GGH64.1e-17794.60U-box domain-containing protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1KKD41.5e-17694.32U-box domain-containing protein 4-like OS=Cucurbita maxima OX=3661 GN=LOC1114965... [more]
A0A6J1HEZ85.9e-17694.89U-box domain-containing protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
Match NameE-valueIdentityDescription
XP_004142891.15.8e-186100.00U-box domain-containing protein 4 [Cucumis sativus] >KGN62451.1 hypothetical pro... [more]
XP_038886361.11.2e-18398.86U-box domain-containing protein 4-like [Benincasa hispida][more]
XP_008444561.11.1e-17998.55PREDICTED: U-box domain-containing protein 4 [Cucumis melo][more]
XP_022951022.18.4e-17794.60U-box domain-containing protein 4-like [Cucurbita moschata] >KAG7020606.1 U-box ... [more]
XP_023536752.11.9e-17694.32U-box domain-containing protein 4-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G01400.11.3e-14881.23ARM repeat superfamily protein [more]
AT5G58680.15.7e-13173.45ARM repeat superfamily protein [more]
AT4G16490.15.2e-7653.58ARM repeat superfamily protein [more]
AT2G23140.18.3e-6646.38RING/U-box superfamily protein with ARM repeat domain [more]
AT2G23140.28.3e-6646.38RING/U-box superfamily protein with ARM repeat domain [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000225ArmadilloSMARTSM00185arm_5coord: 255..295
e-value: 6.4
score: 14.8
coord: 214..254
e-value: 20.0
score: 10.8
coord: 297..337
e-value: 500.0
score: 0.1
coord: 91..131
e-value: 0.0019
score: 27.4
coord: 132..172
e-value: 0.0043
score: 26.3
coord: 173..213
e-value: 0.18
score: 20.9
IPR000225ArmadilloPFAMPF00514Armcoord: 174..213
e-value: 2.8E-6
score: 27.2
coord: 133..171
e-value: 3.4E-6
score: 26.9
coord: 92..130
e-value: 4.9E-6
score: 26.4
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 102..144
score: 10.2724
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 143..185
score: 8.6624
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 42..346
e-value: 5.5E-52
score: 178.8
NoneNo IPR availablePANTHERPTHR23315:SF313U-BOX DOMAIN-CONTAINING PROTEIN 4-LIKE ISOFORM X1coord: 1..345
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 1..345
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 59..333

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G18570.1CSPI02G18570.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding