CSPI02G18290 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G18290
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionSqualene monooxygenase
LocationChr2: 16954770 .. 16958589 (-)
RNA-Seq ExpressionCSPI02G18290
SyntenyCSPI02G18290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATCTCATTCAATAAATGTTCAAATGGCACAAATCACTCACTAACAAAATAGAAACGGGTATGTAATTTAATTAAGTTATGATTAGGCGTAACAATTTTGAAACAAATCCAACGCGAACTGTTCATGGAAGGATAGGAAGGAGAGGGAGTGAATGAATGAATGTAATGTATGGATGTGGTTCACTCTCCGCCTCACAATTTCTATAAAAACCATTACTCTCTTTTTCAATTTTCACTTCTCATAACCAATTCTTTTTTCGTCGTCGCCGCCTCGGGGATCTTCCCTGAGGTTGACGTGATTTGCCATTCTCTCCTACTACGAAACGATGGTGGATCACTGCACCTTCGGCTGGATCTTCTCCGCTTTCCTTGCCTTCGTCATTGCCTTCTCTTTCTTCCTCTCTCCTAGGAAGAATCGCCGAGGCCGGGGAACCAATTCCACGCCGAGGAGGGATTGTCTCAGCAGCTCTGCAACTACCAACGGAGAATGTAGATCCGTCGACGGCGACGCCGACGTCATCATTGTTGGAGCCGGTGTTGCCGGTTCCGCTCTTGCTCACACTCTCGGCAAGGTGTACAATCGGTTTCTTCTTCTTTTTTTCTTTTTTTTTTTTAATTCTTTTTCTTTCTTAGTAAGTATTCGATTATTAGTAGAAGTTGATATTTGATTCACAGAAACCCTTTGAATGATGAAGATTTATGGAAGTCATTGAAAACTTGGGGGTTGTAAATTGCTCTTCCATGTAACCAATGACATGACGGGATGATTGCTTTTAGTTTCTTTCTTTCTTATTGGTGCAAATAATTCTTTTTAAAAAATTATACAGCCACCTTTCGATTTTTATAATGGATAAAATCACTGCATCGTATTTTGATGCAAATTCAATTTGATTATTGTTGACCAAAAGAGAAGGTTGGAATTTATATTTTAAAACTTAACCAAAAGTTCAATTTGGTTTGTTTAGTTCATGGGATTGTTGTTCATCATTAATTGTCCTTTCAAAGGTCAATTATCATTTCAAAAGTTAGGTGGCTAAACTTAAAAAAAGGGAAAGAAAAAGGTTTGTACATATATGTATATTTTTTCTCTCTCTTTTCGTTATTTTTACCATGGAAGCAAAGCAAAGCTGATGACATAAAAATTGGTAGGATGGTCGTCGAGTTCACGTGATTGAAAGAGACTTGACAGAGCCTGATAGGATTGTTGGTGAATTATTGCAGCCTGGTGGCTATCTAAAATTGATTGAGTTAGGACTGCAAGGTTTGTAATTACTCATGGCAAATTATTTTACTGTCTTTTTTTTTTTTTTTCGTTTCCTAAAAGTAAAAAATATTCATCAATTAATTTAGGGAAAAATATGTTTGTAATACAGATTGCGTTGAGGAAATTGATGCACAGAAGGTGTATGGCTATGCTCTTTTCAAAGATGGAAAGAGCACTCGACTTTCTTACCCATTGGAAAATTTTCAATCTGATGTATCTGGAAGAAGCTTCCACAATGGACGCTTCATACAAAGAATGAGGGAGAAGGCTGCTTTTCTTCCCAAGTATGTTCTTTTCGTCTTTTTTTTTTTCTGCTTCATTTTATGAAATTAATTTTACTGTAATTCATCAATGTAAGCTTTTGGGTTGGCCATTATAACAATCACTCTTGAAGATCTTACAAAGTTTCTATGTTAAATGTTACATTTGTTCTCTTGAAGTACTGATTTTGTGTACACATTTTTGTATTTTGCAATTTGTGTCCTTCTGAATTGTTCTATGATACGAAGCTTTTGCTCTTGAAACCTTAGGGATTTGATTGTATTGTCAAGAAGATTTGGACAGTTTGGGGTAGAAATTATTGTTACTTCCATAAAATTTCAGACAAATTTCTTAGGTTTGGTTTCGCCCTATTGAATCTGGTTGAAGTCATAGGGAAAAACATTCACCATTGATACTCGTGTATTTCTTGTGATTGGATTAATGAATAACCACTCATGAACAAAACTTTTTCCATGCTCTGTTTCCGTTTCTTGTAGTGTGAGATTGGAGCAAGGGACAGTTACTTCGTTGCTGGAAGAAAAGGGGACGATCACAGGTGTGCAGTATAAGTCCAAAAATGGTGAACAAAAAACAGCATATGCCCCTCTGACCATTGTTTGTGATGGTTGCTTCTCAAATTTGCGCCGCTCTCTTTGCAACCCTATGGTAAGGTGGCTTCCATTATACGCAGTGGTACTCCAAAATAGACATTTACTCTCTCTTAGGATTTGTGATTGGCTGCAGAATGATTTGCCAACTGCATGAAAACAGTGAATAAGTTTTTCAACTTCTGAGCCCTTTTATCTGCTCTACTTTTGCAGGTTGATGTTCCATCTTGTTTTGTGGGATTGGTTCTGGAGAATTGCCAACTTCCTTACGCAAATCTTGGGCATGTTGTTCTTGGAGATCCTTCCCCTATTTTGTTCTATCCCATCAGCAGCACCGAGATCCGTTGTCTGGTTGATGTTCCTGGTCAGAAGGTTCCTTCTATATCAAATGGTGAAATGGAAAAGTATTTGAAGACCGTAGTCGCTCCCCAGGTACAATTTTTTCATCTCTCATTTCATGCCAGACCATGTGTTCTAGCGCTTTGAACTGATGAATTTACCATTACTTTTGTAGGTTCCTCCGCAAATCCACGATGCCTTTATAGCTGCTATTGAGAAGGGTAATATAAGGACAATGCCGAATAGAAGCATGCCTGCCGCTCCCCAGCCGACACCCGGTGCCTTACTGATGGGTGATGCATTCAACATGCGTCACCCTCTCACCGGTGGAGGAATGACAGTAGCTTTGTCCGATATTGTTGTGCTCAGGAACCTCCTCAAGCCATTGAAAGACTTGAATGATGCACCAACTCTCTGCAAATATCTAGAATCCTTTTACACTCTGAGAAAGGTATTAACTTTAGTTTATGTTGTTGCAATCCTACAAGCCTTTATAAGTAAAATCTGTAATATATTTGTCGTAAACTGCAGCCTGTGGCTTCTACAATCAACACATTGGCGGGGGCATTATACAAGGTGTTTTGTGCATCGTCAGATCAAGCCAGGAAGGAAATGAGACAAGCTTGCTTTGATTATTTGAGCCTTGGAGGAATATTCTCAAATGGGCCTGTCTCCTTGCTTTCGGGATTGAATCCTCGCCCATTGAGTCTCGTTTTCCATTTCTTTGCGGTTGCAATATATGGAGTTGGTCGCTTACTGCTTCCATTTCCTTCTCCGAAAGGCATCTGGATTGGAGCAAGATTGGTCTATGTGAGTTTTGAACTAACAAAACACATCTTTTCATATTCTCCTCTTGAACAAGTAATGAGTAATAAGAAAATGAATTGGGTTTGTTTTGTTTGTTGTAGAGCGCATCAGGAATCATATTTCCAATAATAAAAGCGGAAGGAGTTAGGCAAATGTTCTTCCCAGCAACTGTTCCTGCTTACTACAGAACCCCACCAGTTTTTAACTCATAGCATCAGAGGGATGACATACACAAAAGAAAGATCAAATTGGGCATTGTTTATATCATAATTCCCGAGGAAGAAGAGGCATAACATGGAAAAGACAAGTCTTTTTTCCTTTTTTTTTTTTGTGTGTACGTGTGTGTGTGTGTTAGATTTTCGTTTTAAATTGTGGGTTAATAAAACTTTGTACTTGTGATATGGAGTCAGGAGTGTAAATATTAATTCGTTTCTTTCAACAGCCCCCAAACATTAATGTATCGACAAATTGTTTTGAGAACACTTTAGTAGAGTAAACAGACTTCTCCTTGTTTAGTTTGGCAATATTTTAAATTCATGAT

mRNA sequence

AATCTCATTCAATAAATGTTCAAATGGCACAAATCACTCACTAACAAAATAGAAACGGGTATGTAATTTAATTAAGTTATGATTAGGCGTAACAATTTTGAAACAAATCCAACGCGAACTGTTCATGGAAGGATAGGAAGGAGAGGGAGTGAATGAATGAATGTAATGTATGGATGTGGTTCACTCTCCGCCTCACAATTTCTATAAAAACCATTACTCTCTTTTTCAATTTTCACTTCTCATAACCAATTCTTTTTTCGTCGTCGCCGCCTCGGGGATCTTCCCTGAGGTTGACGTGATTTGCCATTCTCTCCTACTACGAAACGATGGTGGATCACTGCACCTTCGGCTGGATCTTCTCCGCTTTCCTTGCCTTCGTCATTGCCTTCTCTTTCTTCCTCTCTCCTAGGAAGAATCGCCGAGGCCGGGGAACCAATTCCACGCCGAGGAGGGATTGTCTCAGCAGCTCTGCAACTACCAACGGAGAATGTAGATCCGTCGACGGCGACGCCGACGTCATCATTGTTGGAGCCGGTGTTGCCGGTTCCGCTCTTGCTCACACTCTCGGCAAGGATGGTCGTCGAGTTCACGTGATTGAAAGAGACTTGACAGAGCCTGATAGGATTGTTGGTGAATTATTGCAGCCTGGTGGCTATCTAAAATTGATTGAGTTAGGACTGCAAGATTGCGTTGAGGAAATTGATGCACAGAAGGTGTATGGCTATGCTCTTTTCAAAGATGGAAAGAGCACTCGACTTTCTTACCCATTGGAAAATTTTCAATCTGATGTATCTGGAAGAAGCTTCCACAATGGACGCTTCATACAAAGAATGAGGGAGAAGGCTGCTTTTCTTCCCAATGTGAGATTGGAGCAAGGGACAGTTACTTCGTTGCTGGAAGAAAAGGGGACGATCACAGGTGTGCAGTATAAGTCCAAAAATGGTGAACAAAAAACAGCATATGCCCCTCTGACCATTGTTTGTGATGGTTGCTTCTCAAATTTGCGCCGCTCTCTTTGCAACCCTATGGTTGATGTTCCATCTTGTTTTGTGGGATTGGTTCTGGAGAATTGCCAACTTCCTTACGCAAATCTTGGGCATGTTGTTCTTGGAGATCCTTCCCCTATTTTGTTCTATCCCATCAGCAGCACCGAGATCCGTTGTCTGGTTGATGTTCCTGGTCAGAAGGTTCCTTCTATATCAAATGGTGAAATGGAAAAGTATTTGAAGACCGTAGTCGCTCCCCAGGTTCCTCCGCAAATCCACGATGCCTTTATAGCTGCTATTGAGAAGGGTAATATAAGGACAATGCCGAATAGAAGCATGCCTGCCGCTCCCCAGCCGACACCCGGTGCCTTACTGATGGGTGATGCATTCAACATGCGTCACCCTCTCACCGGTGGAGGAATGACAGTAGCTTTGTCCGATATTGTTGTGCTCAGGAACCTCCTCAAGCCATTGAAAGACTTGAATGATGCACCAACTCTCTGCAAATATCTAGAATCCTTTTACACTCTGAGAAAGCCTGTGGCTTCTACAATCAACACATTGGCGGGGGCATTATACAAGGTGTTTTGTGCATCGTCAGATCAAGCCAGGAAGGAAATGAGACAAGCTTGCTTTGATTATTTGAGCCTTGGAGGAATATTCTCAAATGGGCCTGTCTCCTTGCTTTCGGGATTGAATCCTCGCCCATTGAGTCTCGTTTTCCATTTCTTTGCGGTTGCAATATATGGAGTTGGTCGCTTACTGCTTCCATTTCCTTCTCCGAAAGGCATCTGGATTGGAGCAAGATTGGTCTATAGCGCATCAGGAATCATATTTCCAATAATAAAAGCGGAAGGAGTTAGGCAAATGTTCTTCCCAGCAACTGTTCCTGCTTACTACAGAACCCCACCAGTTTTTAACTCATAGCATCAGAGGGATGACATACACAAAAGAAAGATCAAATTGGGCATTGTTTATATCATAATTCCCGAGGAAGAAGAGGCATAACATGGAAAAGACAAGTCTTTTTTCCTTTTTTTTTTTTGTGTGTACGTGTGTGTGTGTGTTAGATTTTCGTTTTAAATTGTGGGTTAATAAAACTTTGTACTTGTGATATGGAGTCAGGAGTGTAAATATTAATTCGTTTCTTTCAACAGCCCCCAAACATTAATGTATCGACAAATTGTTTTGAGAACACTTTAGTAGAGTAAACAGACTTCTCCTTGTTTAGTTTGGCAATATTTTAAATTCATGAT

Coding sequence (CDS)

ATGGTGGATCACTGCACCTTCGGCTGGATCTTCTCCGCTTTCCTTGCCTTCGTCATTGCCTTCTCTTTCTTCCTCTCTCCTAGGAAGAATCGCCGAGGCCGGGGAACCAATTCCACGCCGAGGAGGGATTGTCTCAGCAGCTCTGCAACTACCAACGGAGAATGTAGATCCGTCGACGGCGACGCCGACGTCATCATTGTTGGAGCCGGTGTTGCCGGTTCCGCTCTTGCTCACACTCTCGGCAAGGATGGTCGTCGAGTTCACGTGATTGAAAGAGACTTGACAGAGCCTGATAGGATTGTTGGTGAATTATTGCAGCCTGGTGGCTATCTAAAATTGATTGAGTTAGGACTGCAAGATTGCGTTGAGGAAATTGATGCACAGAAGGTGTATGGCTATGCTCTTTTCAAAGATGGAAAGAGCACTCGACTTTCTTACCCATTGGAAAATTTTCAATCTGATGTATCTGGAAGAAGCTTCCACAATGGACGCTTCATACAAAGAATGAGGGAGAAGGCTGCTTTTCTTCCCAATGTGAGATTGGAGCAAGGGACAGTTACTTCGTTGCTGGAAGAAAAGGGGACGATCACAGGTGTGCAGTATAAGTCCAAAAATGGTGAACAAAAAACAGCATATGCCCCTCTGACCATTGTTTGTGATGGTTGCTTCTCAAATTTGCGCCGCTCTCTTTGCAACCCTATGGTTGATGTTCCATCTTGTTTTGTGGGATTGGTTCTGGAGAATTGCCAACTTCCTTACGCAAATCTTGGGCATGTTGTTCTTGGAGATCCTTCCCCTATTTTGTTCTATCCCATCAGCAGCACCGAGATCCGTTGTCTGGTTGATGTTCCTGGTCAGAAGGTTCCTTCTATATCAAATGGTGAAATGGAAAAGTATTTGAAGACCGTAGTCGCTCCCCAGGTTCCTCCGCAAATCCACGATGCCTTTATAGCTGCTATTGAGAAGGGTAATATAAGGACAATGCCGAATAGAAGCATGCCTGCCGCTCCCCAGCCGACACCCGGTGCCTTACTGATGGGTGATGCATTCAACATGCGTCACCCTCTCACCGGTGGAGGAATGACAGTAGCTTTGTCCGATATTGTTGTGCTCAGGAACCTCCTCAAGCCATTGAAAGACTTGAATGATGCACCAACTCTCTGCAAATATCTAGAATCCTTTTACACTCTGAGAAAGCCTGTGGCTTCTACAATCAACACATTGGCGGGGGCATTATACAAGGTGTTTTGTGCATCGTCAGATCAAGCCAGGAAGGAAATGAGACAAGCTTGCTTTGATTATTTGAGCCTTGGAGGAATATTCTCAAATGGGCCTGTCTCCTTGCTTTCGGGATTGAATCCTCGCCCATTGAGTCTCGTTTTCCATTTCTTTGCGGTTGCAATATATGGAGTTGGTCGCTTACTGCTTCCATTTCCTTCTCCGAAAGGCATCTGGATTGGAGCAAGATTGGTCTATAGCGCATCAGGAATCATATTTCCAATAATAAAAGCGGAAGGAGTTAGGCAAATGTTCTTCCCAGCAACTGTTCCTGCTTACTACAGAACCCCACCAGTTTTTAACTCATAG

Protein sequence

MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDGDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDCVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVRLEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSCFVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASSDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLLLPFPSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFNS*
Homology
BLAST of CSPI02G18290 vs. ExPASy Swiss-Prot
Match: O48651 (Squalene monooxygenase SE1 OS=Panax ginseng OX=4054 GN=SQE1 PE=2 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 3.7e-232
Identity = 393/525 (74.86%), Postives = 452/525 (86.10%), Query Frame = 0

Query: 1   MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG 60
           ++D    GWIF+    F++  +F     KN      NST      S+   T     ++ G
Sbjct: 23  LIDQYFLGWIFAFLFGFLLLLNFKRKREKN------NSTEFGTDDSNGYYTP---ENIAG 82

Query: 61  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120
             DVIIVGAGVAGSALA+TL  DGRRVHVIERDLTE DRIVGELLQPGGYLKLIELGL+D
Sbjct: 83  STDVIIVGAGVAGSALAYTLANDGRRVHVIERDLTEQDRIVGELLQPGGYLKLIELGLED 142

Query: 121 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR 180
           CV EIDAQ+V+GYAL+ DGK+TRLSYPLE F SDV+GRSFHNGRF+QRMREKAA LPNVR
Sbjct: 143 CVNEIDAQRVFGYALYMDGKNTRLSYPLEKFHSDVAGRSFHNGRFVQRMREKAASLPNVR 202

Query: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 240
           +EQGTVTSL+E+KG++ GVQYK+K+G++ +A+APLTIVCDGCFSNLRRSLCNP V+VPSC
Sbjct: 203 MEQGTVTSLVEKKGSVKGVQYKTKDGQELSAFAPLTIVCDGCFSNLRRSLCNPKVEVPSC 262

Query: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300
           FVGL+LEN  LP+ N GHV+L DPSPILFY ISSTEIRCLVDVPGQKVP ISNGE+  YL
Sbjct: 263 FVGLILENIDLPHINHGHVILADPSPILFYKISSTEIRCLVDVPGQKVPCISNGELANYL 322

Query: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360
           KTVVAPQVP Q++++FIAA++KGNIRTMPNRSMPA P PTPGALL+GDAFNMRHPLTGGG
Sbjct: 323 KTVVAPQVPKQLYNSFIAAVDKGNIRTMPNRSMPADPHPTPGALLLGDAFNMRHPLTGGG 382

Query: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS 420
           MTVALSDIV++R+LL+PL+DL+D+ TLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 
Sbjct: 383 MTVALSDIVLIRDLLRPLRDLHDSSTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 442

Query: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLLLPFPS 480
           D+AR+EMR ACFDYLSLGGI S GP++LLSGLNPRP+SL  HFFAVAIYGVGRLL+PFPS
Sbjct: 443 DKARQEMRNACFDYLSLGGICSQGPIALLSGLNPRPISLFLHFFAVAIYGVGRLLIPFPS 502

Query: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPV 526
           PK +W+GARL+  ASGIIFPIIK+EG+RQMFFPA VPAYYR PP+
Sbjct: 503 PKRMWLGARLILGASGIIFPIIKSEGLRQMFFPAIVPAYYRAPPI 538

BLAST of CSPI02G18290 vs. ExPASy Swiss-Prot
Match: Q9SM02 (Squalene epoxidase 1 OS=Arabidopsis thaliana OX=3702 GN=SQE1 PE=1 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 1.4e-231
Identity = 396/517 (76.60%), Postives = 452/517 (87.43%), Query Frame = 0

Query: 10  IFSAFLAFVIAFSFFLSPRKN--RRGRGTNSTPRRDCLSSSATTNGECRSVDGDADVIIV 69
           I S  ++FV  + FF+ P++N  R  R T ST   D  S + T        D  ADVI+V
Sbjct: 14  ISSLLISFVAFYGFFVKPKRNGLRHDRKTVSTVTSDVGSVNITG-------DTVADVIVV 73

Query: 70  GAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDCVEEIDA 129
           GAGVAGSALA+TLGKD RRVHVIERDL+EPDRIVGELLQPGGYLKL+ELG++DCVEEIDA
Sbjct: 74  GAGVAGSALAYTLGKDKRRVHVIERDLSEPDRIVGELLQPGGYLKLLELGIEDCVEEIDA 133

Query: 130 QKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVRLEQGTVT 189
           Q+VYGYALFK+GK  RL+YPLE F  DVSGRSFHNGRFIQRMREKAA LPNV+LEQGTV 
Sbjct: 134 QRVYGYALFKNGKRIRLAYPLEKFHEDVSGRSFHNGRFIQRMREKAASLPNVQLEQGTVL 193

Query: 190 SLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSCFVGLVLE 249
           SLLEE GTI GV+YK+K GE++TA+A LTIVCDGCFSNLRRSLCNP V+VPSCFVGLVLE
Sbjct: 194 SLLEENGTIKGVRYKNKAGEEQTAFAALTIVCDGCFSNLRRSLCNPQVEVPSCFVGLVLE 253

Query: 250 NCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLKTVVAPQ 309
           NC LPYAN GHVVL DPSPIL YPISSTE+RCLVDVPGQKVPSI+NGEM+ YLKTVVAPQ
Sbjct: 254 NCNLPYANHGHVVLADPSPILMYPISSTEVRCLVDVPGQKVPSIANGEMKNYLKTVVAPQ 313

Query: 310 VPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGMTVALSD 369
           +P +++D+FIAA++KGNI++MPNRSMPA+P PTPGALLMGDAFNMRHPLTGGGMTVAL+D
Sbjct: 314 MPHEVYDSFIAAVDKGNIKSMPNRSMPASPYPTPGALLMGDAFNMRHPLTGGGMTVALAD 373

Query: 370 IVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASSDQARKEM 429
           IVVLRNLL+PL+DL+D  +LCKYLESFYTLRKPVA+TINTLA ALY+VFC+S ++AR EM
Sbjct: 374 IVVLRNLLRPLRDLSDGASLCKYLESFYTLRKPVAATINTLANALYQVFCSSENEARNEM 433

Query: 430 RQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLLLPFPSPKGIWIG 489
           R+ACFDYL LGG+ ++GPVSLLSGLNPRPL+LV HFFAVA+YGV RLL+PFPSPK IW+G
Sbjct: 434 REACFDYLGLGGMCTSGPVSLLSGLNPRPLTLVCHFFAVAVYGVIRLLIPFPSPKRIWLG 493

Query: 490 ARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPP 525
           A+L+  ASGIIFPIIKAEGVRQMFFPATVPAYY   P
Sbjct: 494 AKLISGASGIIFPIIKAEGVRQMFFPATVPAYYYKAP 523

BLAST of CSPI02G18290 vs. ExPASy Swiss-Prot
Match: B7TWW5 (Squalene monooxygenase SE2 OS=Panax ginseng OX=4054 GN=SQE2 PE=2 SV=2)

HSP 1 Score: 798.5 bits (2061), Expect = 4.6e-230
Identity = 393/526 (74.71%), Postives = 447/526 (84.98%), Query Frame = 0

Query: 10  IFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDC------LSSSATTNGECRSVD---G 69
           +F+A L F      F   R+ RR          +       ++SS+  NG C   D    
Sbjct: 16  MFAATLLFGFVLYLFTLRRRRRRREKKGGAGSMEIINGAYKMTSSSEVNGHCTPEDIAGS 75

Query: 70  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 129
             DVIIVGAGVAGSALA+TL KDGRRVHVIERDLTE DRIVGELLQPGGYLKL+ELGL+D
Sbjct: 76  SDDVIIVGAGVAGSALAYTLAKDGRRVHVIERDLTEQDRIVGELLQPGGYLKLVELGLED 135

Query: 130 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR 189
           CV EIDAQ+V+GYAL+ DGK+TRLSYPLE F +DV+GRSFHNGRFIQRMREKAA LPNVR
Sbjct: 136 CVNEIDAQRVFGYALYMDGKNTRLSYPLEKFHADVAGRSFHNGRFIQRMREKAASLPNVR 195

Query: 190 LEQGTVTSLLEEKGTITGVQYKSKNG-EQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPS 249
           +EQGTVTSL+E+KGT+ GV+YK+KNG E   AYAPLTIVCDGCFSNLR SLCNP VDVPS
Sbjct: 196 MEQGTVTSLVEQKGTVKGVRYKTKNGQEMSAAYAPLTIVCDGCFSNLRHSLCNPKVDVPS 255

Query: 250 CFVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 309
           CFVGL+LEN  LP+ N GHV+L DPSPILFY ISSTEIRCLVDVPGQKVPSI+NGE+  Y
Sbjct: 256 CFVGLILENIDLPHINHGHVILADPSPILFYKISSTEIRCLVDVPGQKVPSIANGELAHY 315

Query: 310 LKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 369
           LKT VAPQ+PP+++ +FIAAI+KG I+TMPNRSMPA P  TPGALL+GDAFNMRHPLTGG
Sbjct: 316 LKTSVAPQIPPELYKSFIAAIDKGKIKTMPNRSMPADPHSTPGALLLGDAFNMRHPLTGG 375

Query: 370 GMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 429
           GMTVALSDIV++R+LL+PL+DL+D+ TLCKYLESFYTLRKPVASTINTLAGALYKVFCAS
Sbjct: 376 GMTVALSDIVLIRDLLRPLRDLHDSSTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 435

Query: 430 SDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLLLPFP 489
            D+AR+EMR ACFDYLSLGGI S GP++LLSGLNPRP+SL FHFFAVAIYGVGRLL+PFP
Sbjct: 436 PDKARQEMRDACFDYLSLGGICSEGPIALLSGLNPRPMSLFFHFFAVAIYGVGRLLIPFP 495

Query: 490 SPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPV 526
           SP+ +W+GARL+  ASGIIFPIIK+EGVRQMFFPATVPAYYR PP+
Sbjct: 496 SPRKMWLGARLISGASGIIFPIIKSEGVRQMFFPATVPAYYRAPPI 541

BLAST of CSPI02G18290 vs. ExPASy Swiss-Prot
Match: Q8VYH2 (Squalene epoxidase 3 OS=Arabidopsis thaliana OX=3702 GN=SQE3 PE=1 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 6.4e-224
Identity = 389/528 (73.67%), Postives = 443/528 (83.90%), Query Frame = 0

Query: 2   VDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG- 61
           VDHC     F A L     F+F L     RR +  +         S    NG      G 
Sbjct: 7   VDHCILTTTFVASL-----FAFLLLYVLRRRSKTIH--------GSVNVRNGTLTVKSGT 66

Query: 62  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 121
           D D+IIVGAGVAG+ALAHTLGK+GRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGL+D
Sbjct: 67  DVDIIIVGAGVAGAALAHTLGKEGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED 126

Query: 122 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR 181
           CV++IDAQ+V GYALFKDGK T+LSYPL+ F SDV+GRSFHNGRF+QRMREKA+ LPNVR
Sbjct: 127 CVKDIDAQRVLGYALFKDGKHTKLSYPLDQFDSDVAGRSFHNGRFVQRMREKASLLPNVR 186

Query: 182 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 241
           +EQGTVTSL+EE G I GVQYK+K+G++  ++APLTIVCDGCFSNLRRSLC P V+VPS 
Sbjct: 187 MEQGTVTSLVEENGIIKGVQYKTKDGQELKSFAPLTIVCDGCFSNLRRSLCKPKVEVPSN 246

Query: 242 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 301
           FVGLVLENC+LP+ N GHVVLGDPSPILFYPISS+E+RCLVDVPG K+PS+++GEM  +L
Sbjct: 247 FVGLVLENCELPFPNHGHVVLGDPSPILFYPISSSEVRCLVDVPGSKLPSVASGEMAHHL 306

Query: 302 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 361
           KT+VAPQVPPQI DAFI+A+EKGNIRTMPNRSMPA P  TPGALL+GDAFNMRHPLTGGG
Sbjct: 307 KTMVAPQVPPQIRDAFISAVEKGNIRTMPNRSMPADPIHTPGALLLGDAFNMRHPLTGGG 366

Query: 362 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS 421
           MTVALSDIV+LR+LL PL DL +  +L KY+ESFYTLRKPVASTINTLAGALYKVF AS 
Sbjct: 367 MTVALSDIVILRDLLNPLVDLTNKESLSKYIESFYTLRKPVASTINTLAGALYKVFLASP 426

Query: 422 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLLLPFPS 481
           D AR EMR+ACFDYLSLGG+ S+GPV+LLSGLNPRP+SLV HFFAVAI+GVGRLL+P PS
Sbjct: 427 DDARSEMRRACFDYLSLGGVCSSGPVALLSGLNPRPMSLVLHFFAVAIFGVGRLLVPLPS 486

Query: 482 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFNS 529
            K +W+GARL+ SASGIIFPIIKAEGVRQMFFP T+PA YR PP  +S
Sbjct: 487 VKRLWLGARLISSASGIIFPIIKAEGVRQMFFPRTIPAIYRAPPTPSS 521

BLAST of CSPI02G18290 vs. ExPASy Swiss-Prot
Match: O81000 (Squalene epoxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SQE2 PE=1 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 1.6e-222
Identity = 381/513 (74.27%), Postives = 436/513 (84.99%), Query Frame = 0

Query: 12  SAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDGDADVIIVGAGV 71
           ++   F++ +    S  KN++ RG     + D +S +  T      VD   DVIIVGAGV
Sbjct: 79  ASLFTFLLLYILRRSSNKNKKNRGL-VVSQNDTVSKNLET-----EVDSGTDVIIVGAGV 138

Query: 72  AGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDCVEEIDAQKVY 131
           AGSALAHTLGK+GRRVHVIERD +E DRIVGELLQPGGYLKLIELGL+DCV++IDAQ+V 
Sbjct: 139 AGSALAHTLGKEGRRVHVIERDFSEQDRIVGELLQPGGYLKLIELGLEDCVKKIDAQRVL 198

Query: 132 GYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVRLEQGTVTSLLE 191
           GY LFKDGK T+L+YPLE F SDV+GRSFHNGRF+QRMREKA  L NVRLEQGTVTSLLE
Sbjct: 199 GYVLFKDGKHTKLAYPLETFDSDVAGRSFHNGRFVQRMREKALTLSNVRLEQGTVTSLLE 258

Query: 192 EKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSCFVGLVLENCQL 251
           E GTI GV+Y++K G +  ++APLTIVCDGCFSNLRRSLC P VDVPS FVGLVLENC+L
Sbjct: 259 EHGTIKGVRYRTKEGNEFRSFAPLTIVCDGCFSNLRRSLCKPKVDVPSTFVGLVLENCEL 318

Query: 252 PYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLKTVVAPQVPPQ 311
           P+AN GHVVLGDPSPIL YPISS+E+RCLVDVPGQK+P I+NGEM KYLKT VAPQVP +
Sbjct: 319 PFANHGHVVLGDPSPILMYPISSSEVRCLVDVPGQKLPPIANGEMAKYLKTRVAPQVPTK 378

Query: 312 IHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVL 371
           + +AFI A+EKGNIRTMPNRSMPA P PTPGALL+GDAFNMRHPLTGGGMTVAL+DIVVL
Sbjct: 379 VREAFITAVEKGNIRTMPNRSMPADPIPTPGALLLGDAFNMRHPLTGGGMTVALADIVVL 438

Query: 372 RNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASSDQARKEMRQAC 431
           R+LL+P+++LND   L KY+ESFYTLRKPVASTINTLA ALYKVF ASSD+AR EMR+AC
Sbjct: 439 RDLLRPIRNLNDKEALSKYIESFYTLRKPVASTINTLADALYKVFLASSDEARTEMREAC 498

Query: 432 FDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLLLPFPSPKGIWIGARLV 491
           FDYLSLGG+FS+GPV+LLSGLNPRPLSLV HFFAVAIY V RL+LPFPS +  W+GAR++
Sbjct: 499 FDYLSLGGVFSSGPVALLSGLNPRPLSLVLHFFAVAIYAVCRLMLPFPSIESFWLGARII 558

Query: 492 YSASGIIFPIIKAEGVRQMFFPATVPAYYRTPP 525
            SAS IIFPIIKAEGVRQMFFP T+PA YR PP
Sbjct: 559 SSASSIIFPIIKAEGVRQMFFPRTIPAIYRAPP 585

BLAST of CSPI02G18290 vs. ExPASy TrEMBL
Match: A0A0A0LKI1 (Squalene monooxygenase OS=Cucumis sativus OX=3659 GN=Csa_2G353480 PE=3 SV=1)

HSP 1 Score: 1066.6 bits (2757), Expect = 3.3e-308
Identity = 527/528 (99.81%), Postives = 527/528 (99.81%), Query Frame = 0

Query: 1   MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG 60
           MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG
Sbjct: 1   MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG 60

Query: 61  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120
           DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 121 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR 180
           CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR 180

Query: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 240
           LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC
Sbjct: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 240

Query: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300
           FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300

Query: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360
           KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS 420
           MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS 420

Query: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLLLPFPS 480
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLV HFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFNS 529
           PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFNS
Sbjct: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFNS 528

BLAST of CSPI02G18290 vs. ExPASy TrEMBL
Match: A0A5A7V0X5 (Squalene monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001090 PE=3 SV=1)

HSP 1 Score: 1014.2 bits (2621), Expect = 2.0e-292
Identity = 505/528 (95.64%), Postives = 510/528 (96.59%), Query Frame = 0

Query: 1   MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG 60
           MVDHC FGWIFSA LAF IA S FLSP +NRR RGT+STPR   +SSSATTNGECRSVDG
Sbjct: 1   MVDHCAFGWIFSALLAFPIALSLFLSPWRNRRVRGTDSTPRSASVSSSATTNGECRSVDG 60

Query: 61  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120
           DADV+IVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVVIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 121 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR 180
           CVEEIDAQKVYGYALFKDGK+TRLSYPLENF SDVSGRSFHNGRFIQRMREKAA LPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNVR 180

Query: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 240
           LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLC PMVDVPS 
Sbjct: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCTPMVDVPSF 240

Query: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300
           FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300

Query: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360
           KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS 420
           MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420

Query: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLLLPFPS 480
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLV HFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFNS 529
            KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPV NS
Sbjct: 481 LKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVLNS 528

BLAST of CSPI02G18290 vs. ExPASy TrEMBL
Match: A0A1S3BB88 (Squalene monooxygenase OS=Cucumis melo OX=3656 GN=LOC103487810 PE=3 SV=1)

HSP 1 Score: 1014.2 bits (2621), Expect = 2.0e-292
Identity = 505/528 (95.64%), Postives = 510/528 (96.59%), Query Frame = 0

Query: 1   MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG 60
           MVDHC FGWIFSA LAF IA S FLSP +NRR RGT+STPR   +SSSATTNGECRSVDG
Sbjct: 1   MVDHCAFGWIFSALLAFPIALSLFLSPWRNRRVRGTDSTPRSASVSSSATTNGECRSVDG 60

Query: 61  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120
           DADV+IVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVVIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 121 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR 180
           CVEEIDAQKVYGYALFKDGK+TRLSYPLENF SDVSGRSFHNGRFIQRMREKAA LPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNVR 180

Query: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 240
           LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLC PMVDVPS 
Sbjct: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCTPMVDVPSY 240

Query: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300
           FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300

Query: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360
           KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS 420
           MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420

Query: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLLLPFPS 480
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLV HFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFNS 529
            KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPV NS
Sbjct: 481 LKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVLNS 528

BLAST of CSPI02G18290 vs. ExPASy TrEMBL
Match: A0A6J1K5U9 (Squalene monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111491959 PE=3 SV=1)

HSP 1 Score: 991.9 bits (2563), Expect = 1.0e-285
Identity = 490/528 (92.80%), Postives = 506/528 (95.83%), Query Frame = 0

Query: 1   MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG 60
           MVD+C FGWI +A L   IA SFF+SPR+NRRG G +STPR + + SS+TTNGECRSVDG
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRG-GADSTPRSEGVRSSSTTNGECRSVDG 60

Query: 61  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120
           DADVIIVGAGVAGSALAHTLGKDGR VHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 121 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR 180
           CVEEIDAQKVYGYALFKDGK+T+LSYPLE FQSDVSGRSFHNGRFIQRMREKAA LPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVR 180

Query: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 240
           LEQGTVTSLLEEKGTI GVQYKSKNGE+KTAYAPLTIVCDGCFSNLRRSLC PMVDVPSC
Sbjct: 181 LEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSC 240

Query: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300
           FVGLVLENCQLP+AN GHVVLGDPSPILFYPISSTEIRCLVDVPGQK+PSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKIPSISNGEMEKYL 300

Query: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360
           KT+VAPQVPPQIHDAFIAAI+KGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS 420
           MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420

Query: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLLLPFPS 480
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLV HFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFNS 529
           PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYR+PPV  S
Sbjct: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVHKS 527

BLAST of CSPI02G18290 vs. ExPASy TrEMBL
Match: A0A6J1HEG7 (Squalene monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111462746 PE=3 SV=1)

HSP 1 Score: 986.1 bits (2548), Expect = 5.7e-284
Identity = 488/528 (92.42%), Postives = 505/528 (95.64%), Query Frame = 0

Query: 1   MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG 60
           MVD+C FGWI +A L   IA SFF+SPR+NRRG G +STPR + + SS+TTNGECRSVD 
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRG-GADSTPRSEGVRSSSTTNGECRSVDC 60

Query: 61  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120
           DADVIIVGAGVAGSALAHTLGKDGR VHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 121 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR 180
           CVEEIDAQKVYGYALFKDGK+T+LSYPLE FQSDVSGRSFHNGRFIQRMREKAA LPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVR 180

Query: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 240
           LEQGTVTSLLEEKGTI GVQYKSKNGE+KTA+APLTIVCDGCFSNLRRSLC PMVDVPSC
Sbjct: 181 LEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAHAPLTIVCDGCFSNLRRSLCKPMVDVPSC 240

Query: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300
           FVGLVLENCQLP+AN GHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300

Query: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360
           KT+VAPQVPPQIHDAFIAAI+KGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS 420
           MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420

Query: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLLLPFPS 480
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLV HFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFNS 529
           PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYR+PPV  +
Sbjct: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKT 527

BLAST of CSPI02G18290 vs. NCBI nr
Match: XP_004142907.1 (squalene monooxygenase SE1 [Cucumis sativus] >KGN62420.1 hypothetical protein Csa_018728 [Cucumis sativus])

HSP 1 Score: 1066.6 bits (2757), Expect = 6.8e-308
Identity = 527/528 (99.81%), Postives = 527/528 (99.81%), Query Frame = 0

Query: 1   MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG 60
           MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG
Sbjct: 1   MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG 60

Query: 61  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120
           DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 121 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR 180
           CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR 180

Query: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 240
           LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC
Sbjct: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 240

Query: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300
           FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300

Query: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360
           KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS 420
           MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS 420

Query: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLLLPFPS 480
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLV HFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFNS 529
           PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFNS
Sbjct: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFNS 528

BLAST of CSPI02G18290 vs. NCBI nr
Match: XP_008444517.1 (PREDICTED: squalene monooxygenase-like [Cucumis melo])

HSP 1 Score: 1014.2 bits (2621), Expect = 4.0e-292
Identity = 505/528 (95.64%), Postives = 510/528 (96.59%), Query Frame = 0

Query: 1   MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG 60
           MVDHC FGWIFSA LAF IA S FLSP +NRR RGT+STPR   +SSSATTNGECRSVDG
Sbjct: 1   MVDHCAFGWIFSALLAFPIALSLFLSPWRNRRVRGTDSTPRSASVSSSATTNGECRSVDG 60

Query: 61  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120
           DADV+IVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVVIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 121 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR 180
           CVEEIDAQKVYGYALFKDGK+TRLSYPLENF SDVSGRSFHNGRFIQRMREKAA LPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNVR 180

Query: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 240
           LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLC PMVDVPS 
Sbjct: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCTPMVDVPSY 240

Query: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300
           FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300

Query: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360
           KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS 420
           MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420

Query: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLLLPFPS 480
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLV HFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFNS 529
            KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPV NS
Sbjct: 481 LKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVLNS 528

BLAST of CSPI02G18290 vs. NCBI nr
Match: KAA0060974.1 (squalene monooxygenase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1014.2 bits (2621), Expect = 4.0e-292
Identity = 505/528 (95.64%), Postives = 510/528 (96.59%), Query Frame = 0

Query: 1   MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG 60
           MVDHC FGWIFSA LAF IA S FLSP +NRR RGT+STPR   +SSSATTNGECRSVDG
Sbjct: 1   MVDHCAFGWIFSALLAFPIALSLFLSPWRNRRVRGTDSTPRSASVSSSATTNGECRSVDG 60

Query: 61  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120
           DADV+IVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVVIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 121 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR 180
           CVEEIDAQKVYGYALFKDGK+TRLSYPLENF SDVSGRSFHNGRFIQRMREKAA LPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNVR 180

Query: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 240
           LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLC PMVDVPS 
Sbjct: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCTPMVDVPSF 240

Query: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300
           FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300

Query: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360
           KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS 420
           MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420

Query: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLLLPFPS 480
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLV HFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFNS 529
            KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPV NS
Sbjct: 481 LKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVLNS 528

BLAST of CSPI02G18290 vs. NCBI nr
Match: XP_038886247.1 (squalene monooxygenase SE1-like [Benincasa hispida])

HSP 1 Score: 1012.3 bits (2616), Expect = 1.5e-291
Identity = 498/526 (94.68%), Postives = 509/526 (96.77%), Query Frame = 0

Query: 1   MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG 60
           MVDHC FGWI SA  AFVIA SFFL+PR+NRR  GT+STPR DCL SS+TTNGECRS+DG
Sbjct: 1   MVDHCAFGWILSALFAFVIALSFFLAPRRNRRVEGTHSTPRSDCLRSSSTTNGECRSIDG 60

Query: 61  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120
           DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 121 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR 180
           CVEEIDAQKVYGYALFKDGK+TRLSYPLENF SDVSGRSFHNGRFIQRMREKAA LPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNVR 180

Query: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 240
           LEQGTVTSLLEEKGTI GVQYKSKNGE KTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC
Sbjct: 181 LEQGTVTSLLEEKGTIKGVQYKSKNGEVKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 240

Query: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300
           FVGLVLENCQLP+AN GHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300

Query: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360
           KTVVAPQVP +IHDAF+AAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTVVAPQVPREIHDAFVAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS 420
           MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420

Query: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLLLPFPS 480
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLV HFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVF 527
           PKGIWIGARL+YSASGIIFPIIKAEGVRQMFFPATVPAYYR+PPVF
Sbjct: 481 PKGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVF 526

BLAST of CSPI02G18290 vs. NCBI nr
Match: XP_022996831.1 (squalene monooxygenase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 991.9 bits (2563), Expect = 2.1e-285
Identity = 490/528 (92.80%), Postives = 506/528 (95.83%), Query Frame = 0

Query: 1   MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG 60
           MVD+C FGWI +A L   IA SFF+SPR+NRRG G +STPR + + SS+TTNGECRSVDG
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRG-GADSTPRSEGVRSSSTTNGECRSVDG 60

Query: 61  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120
           DADVIIVGAGVAGSALAHTLGKDGR VHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 121 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR 180
           CVEEIDAQKVYGYALFKDGK+T+LSYPLE FQSDVSGRSFHNGRFIQRMREKAA LPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVR 180

Query: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 240
           LEQGTVTSLLEEKGTI GVQYKSKNGE+KTAYAPLTIVCDGCFSNLRRSLC PMVDVPSC
Sbjct: 181 LEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSC 240

Query: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300
           FVGLVLENCQLP+AN GHVVLGDPSPILFYPISSTEIRCLVDVPGQK+PSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKIPSISNGEMEKYL 300

Query: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360
           KT+VAPQVPPQIHDAFIAAI+KGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS 420
           MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420

Query: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLLLPFPS 480
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLV HFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFNS 529
           PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYR+PPV  S
Sbjct: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVHKS 527

BLAST of CSPI02G18290 vs. TAIR 10
Match: AT1G58440.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 803.5 bits (2074), Expect = 1.0e-232
Identity = 396/517 (76.60%), Postives = 452/517 (87.43%), Query Frame = 0

Query: 10  IFSAFLAFVIAFSFFLSPRKN--RRGRGTNSTPRRDCLSSSATTNGECRSVDGDADVIIV 69
           I S  ++FV  + FF+ P++N  R  R T ST   D  S + T        D  ADVI+V
Sbjct: 14  ISSLLISFVAFYGFFVKPKRNGLRHDRKTVSTVTSDVGSVNITG-------DTVADVIVV 73

Query: 70  GAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDCVEEIDA 129
           GAGVAGSALA+TLGKD RRVHVIERDL+EPDRIVGELLQPGGYLKL+ELG++DCVEEIDA
Sbjct: 74  GAGVAGSALAYTLGKDKRRVHVIERDLSEPDRIVGELLQPGGYLKLLELGIEDCVEEIDA 133

Query: 130 QKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVRLEQGTVT 189
           Q+VYGYALFK+GK  RL+YPLE F  DVSGRSFHNGRFIQRMREKAA LPNV+LEQGTV 
Sbjct: 134 QRVYGYALFKNGKRIRLAYPLEKFHEDVSGRSFHNGRFIQRMREKAASLPNVQLEQGTVL 193

Query: 190 SLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSCFVGLVLE 249
           SLLEE GTI GV+YK+K GE++TA+A LTIVCDGCFSNLRRSLCNP V+VPSCFVGLVLE
Sbjct: 194 SLLEENGTIKGVRYKNKAGEEQTAFAALTIVCDGCFSNLRRSLCNPQVEVPSCFVGLVLE 253

Query: 250 NCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLKTVVAPQ 309
           NC LPYAN GHVVL DPSPIL YPISSTE+RCLVDVPGQKVPSI+NGEM+ YLKTVVAPQ
Sbjct: 254 NCNLPYANHGHVVLADPSPILMYPISSTEVRCLVDVPGQKVPSIANGEMKNYLKTVVAPQ 313

Query: 310 VPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGMTVALSD 369
           +P +++D+FIAA++KGNI++MPNRSMPA+P PTPGALLMGDAFNMRHPLTGGGMTVAL+D
Sbjct: 314 MPHEVYDSFIAAVDKGNIKSMPNRSMPASPYPTPGALLMGDAFNMRHPLTGGGMTVALAD 373

Query: 370 IVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASSDQARKEM 429
           IVVLRNLL+PL+DL+D  +LCKYLESFYTLRKPVA+TINTLA ALY+VFC+S ++AR EM
Sbjct: 374 IVVLRNLLRPLRDLSDGASLCKYLESFYTLRKPVAATINTLANALYQVFCSSENEARNEM 433

Query: 430 RQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLLLPFPSPKGIWIG 489
           R+ACFDYL LGG+ ++GPVSLLSGLNPRPL+LV HFFAVA+YGV RLL+PFPSPK IW+G
Sbjct: 434 REACFDYLGLGGMCTSGPVSLLSGLNPRPLTLVCHFFAVAVYGVIRLLIPFPSPKRIWLG 493

Query: 490 ARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPP 525
           A+L+  ASGIIFPIIKAEGVRQMFFPATVPAYY   P
Sbjct: 494 AKLISGASGIIFPIIKAEGVRQMFFPATVPAYYYKAP 523

BLAST of CSPI02G18290 vs. TAIR 10
Match: AT4G37760.1 (squalene epoxidase 3 )

HSP 1 Score: 778.1 bits (2008), Expect = 4.5e-225
Identity = 389/528 (73.67%), Postives = 443/528 (83.90%), Query Frame = 0

Query: 2   VDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG- 61
           VDHC     F A L     F+F L     RR +  +         S    NG      G 
Sbjct: 7   VDHCILTTTFVASL-----FAFLLLYVLRRRSKTIH--------GSVNVRNGTLTVKSGT 66

Query: 62  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 121
           D D+IIVGAGVAG+ALAHTLGK+GRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGL+D
Sbjct: 67  DVDIIIVGAGVAGAALAHTLGKEGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED 126

Query: 122 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR 181
           CV++IDAQ+V GYALFKDGK T+LSYPL+ F SDV+GRSFHNGRF+QRMREKA+ LPNVR
Sbjct: 127 CVKDIDAQRVLGYALFKDGKHTKLSYPLDQFDSDVAGRSFHNGRFVQRMREKASLLPNVR 186

Query: 182 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 241
           +EQGTVTSL+EE G I GVQYK+K+G++  ++APLTIVCDGCFSNLRRSLC P V+VPS 
Sbjct: 187 MEQGTVTSLVEENGIIKGVQYKTKDGQELKSFAPLTIVCDGCFSNLRRSLCKPKVEVPSN 246

Query: 242 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 301
           FVGLVLENC+LP+ N GHVVLGDPSPILFYPISS+E+RCLVDVPG K+PS+++GEM  +L
Sbjct: 247 FVGLVLENCELPFPNHGHVVLGDPSPILFYPISSSEVRCLVDVPGSKLPSVASGEMAHHL 306

Query: 302 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 361
           KT+VAPQVPPQI DAFI+A+EKGNIRTMPNRSMPA P  TPGALL+GDAFNMRHPLTGGG
Sbjct: 307 KTMVAPQVPPQIRDAFISAVEKGNIRTMPNRSMPADPIHTPGALLLGDAFNMRHPLTGGG 366

Query: 362 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS 421
           MTVALSDIV+LR+LL PL DL +  +L KY+ESFYTLRKPVASTINTLAGALYKVF AS 
Sbjct: 367 MTVALSDIVILRDLLNPLVDLTNKESLSKYIESFYTLRKPVASTINTLAGALYKVFLASP 426

Query: 422 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLLLPFPS 481
           D AR EMR+ACFDYLSLGG+ S+GPV+LLSGLNPRP+SLV HFFAVAI+GVGRLL+P PS
Sbjct: 427 DDARSEMRRACFDYLSLGGVCSSGPVALLSGLNPRPMSLVLHFFAVAIFGVGRLLVPLPS 486

Query: 482 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFNS 529
            K +W+GARL+ SASGIIFPIIKAEGVRQMFFP T+PA YR PP  +S
Sbjct: 487 VKRLWLGARLISSASGIIFPIIKAEGVRQMFFPRTIPAIYRAPPTPSS 521

BLAST of CSPI02G18290 vs. TAIR 10
Match: AT2G22830.1 (squalene epoxidase 2 )

HSP 1 Score: 773.5 bits (1996), Expect = 1.1e-223
Identity = 381/513 (74.27%), Postives = 436/513 (84.99%), Query Frame = 0

Query: 12  SAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDGDADVIIVGAGV 71
           ++   F++ +    S  KN++ RG     + D +S +  T      VD   DVIIVGAGV
Sbjct: 79  ASLFTFLLLYILRRSSNKNKKNRGL-VVSQNDTVSKNLET-----EVDSGTDVIIVGAGV 138

Query: 72  AGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDCVEEIDAQKVY 131
           AGSALAHTLGK+GRRVHVIERD +E DRIVGELLQPGGYLKLIELGL+DCV++IDAQ+V 
Sbjct: 139 AGSALAHTLGKEGRRVHVIERDFSEQDRIVGELLQPGGYLKLIELGLEDCVKKIDAQRVL 198

Query: 132 GYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVRLEQGTVTSLLE 191
           GY LFKDGK T+L+YPLE F SDV+GRSFHNGRF+QRMREKA  L NVRLEQGTVTSLLE
Sbjct: 199 GYVLFKDGKHTKLAYPLETFDSDVAGRSFHNGRFVQRMREKALTLSNVRLEQGTVTSLLE 258

Query: 192 EKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSCFVGLVLENCQL 251
           E GTI GV+Y++K G +  ++APLTIVCDGCFSNLRRSLC P VDVPS FVGLVLENC+L
Sbjct: 259 EHGTIKGVRYRTKEGNEFRSFAPLTIVCDGCFSNLRRSLCKPKVDVPSTFVGLVLENCEL 318

Query: 252 PYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLKTVVAPQVPPQ 311
           P+AN GHVVLGDPSPIL YPISS+E+RCLVDVPGQK+P I+NGEM KYLKT VAPQVP +
Sbjct: 319 PFANHGHVVLGDPSPILMYPISSSEVRCLVDVPGQKLPPIANGEMAKYLKTRVAPQVPTK 378

Query: 312 IHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVL 371
           + +AFI A+EKGNIRTMPNRSMPA P PTPGALL+GDAFNMRHPLTGGGMTVAL+DIVVL
Sbjct: 379 VREAFITAVEKGNIRTMPNRSMPADPIPTPGALLLGDAFNMRHPLTGGGMTVALADIVVL 438

Query: 372 RNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASSDQARKEMRQAC 431
           R+LL+P+++LND   L KY+ESFYTLRKPVASTINTLA ALYKVF ASSD+AR EMR+AC
Sbjct: 439 RDLLRPIRNLNDKEALSKYIESFYTLRKPVASTINTLADALYKVFLASSDEARTEMREAC 498

Query: 432 FDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLLLPFPSPKGIWIGARLV 491
           FDYLSLGG+FS+GPV+LLSGLNPRPLSLV HFFAVAIY V RL+LPFPS +  W+GAR++
Sbjct: 499 FDYLSLGGVFSSGPVALLSGLNPRPLSLVLHFFAVAIYAVCRLMLPFPSIESFWLGARII 558

Query: 492 YSASGIIFPIIKAEGVRQMFFPATVPAYYRTPP 525
            SAS IIFPIIKAEGVRQMFFP T+PA YR PP
Sbjct: 559 SSASSIIFPIIKAEGVRQMFFPRTIPAIYRAPP 585

BLAST of CSPI02G18290 vs. TAIR 10
Match: AT5G24150.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 505.8 bits (1301), Expect = 4.3e-143
Identity = 247/467 (52.89%), Postives = 331/467 (70.88%), Query Frame = 0

Query: 59  DGDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGL 118
           DG  DVIIVGAGV GSALA+ L KDGRRVHVIERDL EP+RI+GE +QPGG L L +LGL
Sbjct: 43  DGATDVIIVGAGVGGSALAYALAKDGRRVHVIERDLREPERIMGEFMQPGGRLMLSKLGL 102

Query: 119 QDCVEEIDAQKVYGYALFKDGKSTRLSYPLE--NFQSDVSGRSFHNGRFIQRMREKAAFL 178
           +DC+E IDAQK  G  ++KDGK    S+P++  NF  D S RSFHNGRF+QR+R+KA+ L
Sbjct: 103 EDCLEGIDAQKATGMTVYKDGKEAVASFPVDNNNFPFDPSARSFHNGRFVQRLRQKASSL 162

Query: 179 PNVRLEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVD 238
           PNVRLE+GTV SL+EEKG I GV YK+  GE+ TA APLT+VCDGC+SNLRRSL +   +
Sbjct: 163 PNVRLEEGTVKSLIEEKGVIKGVTYKNSAGEETTALAPLTVVCDGCYSNLRRSLNDNNAE 222

Query: 239 VPSCFVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEM 298
           V S  VG + +NCQL       +++  PS  + Y ISST++RC+ +V    +PSISNGEM
Sbjct: 223 VLSYQVGFISKNCQLEEPEKLKLIMSKPSFTMLYQISSTDVRCVFEVLPNNIPSISNGEM 282

Query: 299 EKYLKTVVAPQVPPQIHDAFIAAIEKG-NIRTMPNRSMPAAPQPTPGALLMGDAFNMRHP 358
             ++K  +APQVP ++   F+  I++G +I+ MP + M A      G +L+GDAFNMRHP
Sbjct: 283 ATFVKNTIAPQVPLKLRKIFLKGIDEGEHIKAMPTKKMTATLSEKKGVILLGDAFNMRHP 342

Query: 359 LTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKV 418
               GM V LSDI++LR LL+PL +L +A  + + ++SFY +RKP+++T+NTL  A  +V
Sbjct: 343 AIASGMMVLLSDILILRRLLQPLSNLGNAQKISQVIKSFYDIRKPMSATVNTLGNAFSQV 402

Query: 419 FCASSDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRLL 478
             AS+D+A++ MRQ C+DYLS GG  ++G ++LL G+NPRP+SL++H  A+ +  +G LL
Sbjct: 403 LVASTDEAKEAMRQGCYDYLSSGGFRTSGMMALLGGMNPRPISLIYHLCAITLSSIGHLL 462

Query: 479 LPFPSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRT 523
            PFPSP  IW   RL   A  ++ P +KAEGV QM FP    AY ++
Sbjct: 463 SPFPSPLRIWHSLRLFGLAMKMLVPHLKAEGVSQMLFPVNAAAYSKS 509

BLAST of CSPI02G18290 vs. TAIR 10
Match: AT5G24160.1 (squalene monoxygenase 6 )

HSP 1 Score: 494.6 bits (1272), Expect = 1.0e-139
Identity = 241/468 (51.50%), Postives = 331/468 (70.73%), Query Frame = 0

Query: 59  DGDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGL 118
           DG ADVIIVGAGV GSALA+ L KDGRRVHVIERD+ EP+R++GE +QPGG L L +LGL
Sbjct: 43  DGAADVIIVGAGVGGSALAYALAKDGRRVHVIERDMREPERMMGEFMQPGGRLMLSKLGL 102

Query: 119 QDCVEEIDAQKVYGYALFKDGKSTRLSYPLE--NFQSDVSGRSFHNGRFIQRMREKAAFL 178
           QDC+E+IDAQK  G A++KDGK     +P++  NF  + S RSFHNGRF+Q++R KA  L
Sbjct: 103 QDCLEDIDAQKATGLAVYKDGKEADAPFPVDNNNFSYEPSARSFHNGRFVQQLRRKAFSL 162

Query: 179 PNVRLEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNP-MV 238
            NVRLE+GTV SLLEEKG + GV YK+K GE+ TA APLT+VCDGC+SNLRRSL +    
Sbjct: 163 SNVRLEEGTVKSLLEEKGVVKGVTYKNKEGEETTALAPLTVVCDGCYSNLRRSLNDDNNA 222

Query: 239 DVPSCFVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGE 298
           ++ S  VG + +NC+L      H++L  PS  + Y ISST++RC  +V  +  PSI+NGE
Sbjct: 223 EIMSYIVGYISKNCRLEEPEKLHLILSKPSFTMVYQISSTDVRCGFEVLPENFPSIANGE 282

Query: 299 MEKYLKTVVAPQVPPQIHDAFIAAIEKG-NIRTMPNRSMPAAPQPTPGALLMGDAFNMRH 358
           M  ++K  + PQVPP++   F+  I++G +I+ +P + M +      G +++GDAFNMRH
Sbjct: 283 MSTFMKNTIVPQVPPKLRKIFLKGIDEGAHIKVVPAKRMTSTLSKKKGVIVLGDAFNMRH 342

Query: 359 PLTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYK 418
           P+   GM V LSDI++LR LL+PL +L DA  + + + SFY +RKP+++T+NTL  A  +
Sbjct: 343 PVVASGMMVLLSDILILRRLLQPLSNLGDANKVSEVINSFYDIRKPMSATVNTLGNAFSQ 402

Query: 419 VFCASSDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVFHFFAVAIYGVGRL 478
           V   S+D+A++ MRQ  +DYL  GG  ++G ++LL G+NPRPLSLV+H  A+ +  +G+L
Sbjct: 403 VLIGSTDEAKEAMRQGVYDYLCSGGFRTSGMMALLGGMNPRPLSLVYHLCAITLSSIGQL 462

Query: 479 LLPFPSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRT 523
           L PFPSP  IW   +L   A  ++ P +KAEGV QM FPA   AY+++
Sbjct: 463 LSPFPSPLRIWHSLKLFGLAMKMLVPNLKAEGVSQMLFPANAAAYHKS 510

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O486513.7e-23274.86Squalene monooxygenase SE1 OS=Panax ginseng OX=4054 GN=SQE1 PE=2 SV=1[more]
Q9SM021.4e-23176.60Squalene epoxidase 1 OS=Arabidopsis thaliana OX=3702 GN=SQE1 PE=1 SV=1[more]
B7TWW54.6e-23074.71Squalene monooxygenase SE2 OS=Panax ginseng OX=4054 GN=SQE2 PE=2 SV=2[more]
Q8VYH26.4e-22473.67Squalene epoxidase 3 OS=Arabidopsis thaliana OX=3702 GN=SQE3 PE=1 SV=1[more]
O810001.6e-22274.27Squalene epoxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SQE2 PE=1... [more]
Match NameE-valueIdentityDescription
A0A0A0LKI13.3e-30899.81Squalene monooxygenase OS=Cucumis sativus OX=3659 GN=Csa_2G353480 PE=3 SV=1[more]
A0A5A7V0X52.0e-29295.64Squalene monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold5... [more]
A0A1S3BB882.0e-29295.64Squalene monooxygenase OS=Cucumis melo OX=3656 GN=LOC103487810 PE=3 SV=1[more]
A0A6J1K5U91.0e-28592.80Squalene monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111491959 PE=3 SV=1[more]
A0A6J1HEG75.7e-28492.42Squalene monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111462746 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004142907.16.8e-30899.81squalene monooxygenase SE1 [Cucumis sativus] >KGN62420.1 hypothetical protein Cs... [more]
XP_008444517.14.0e-29295.64PREDICTED: squalene monooxygenase-like [Cucumis melo][more]
KAA0060974.14.0e-29295.64squalene monooxygenase-like [Cucumis melo var. makuwa][more]
XP_038886247.11.5e-29194.68squalene monooxygenase SE1-like [Benincasa hispida][more]
XP_022996831.12.1e-28592.80squalene monooxygenase-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G58440.11.0e-23276.60FAD/NAD(P)-binding oxidoreductase family protein [more]
AT4G37760.14.5e-22573.67squalene epoxidase 3 [more]
AT2G22830.11.1e-22374.27squalene epoxidase 2 [more]
AT5G24150.14.3e-14352.89FAD/NAD(P)-binding oxidoreductase family protein [more]
AT5G24160.11.0e-13951.50squalene monoxygenase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00420RNGMNOXGNASEcoord: 212..227
score: 31.25
coord: 63..85
score: 43.04
NoneNo IPR availablePFAMPF13450NAD_binding_8coord: 66..92
e-value: 1.7E-5
score: 24.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 30..49
NoneNo IPR availablePANTHERPTHR10835:SF15SQUALENE EPOXIDASE 2, MITOCHONDRIALcoord: 10..309
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 59..252
e-value: 1.8E-25
score: 91.9
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 62..233
IPR013698Squalene epoxidasePFAMPF08491SEcoord: 213..308
e-value: 2.7E-39
score: 134.9
IPR040125Squalene monooxygenasePANTHERPTHR10835SQUALENE MONOOXYGENASEcoord: 10..309

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G18290.1CSPI02G18290.1mRNA
CSPI02G18290.2CSPI02G18290.2mRNA
CSPI02G18290.3CSPI02G18290.3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016126 sterol biosynthetic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0004506 squalene monooxygenase activity