CSPI02G17490 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G17490
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPectate lyase
LocationChr2: 16494368 .. 16496508 (-)
RNA-Seq ExpressionCSPI02G17490
SyntenyCSPI02G17490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAACTCTCCCAAATCTCAATCAATCCTTTTCTCTGCTAATATTCACACAAGAAAGAAAGAAAGAAAGAAAGAGAGAGAGAGAGAGAGCTATGGAAAATTCCAATTCTGGTGTGATTATGATGAGTATAAAGTTATTAGTATTGAGTATTGTTATGCTCCAAATGAGCTCCATGTCATCGTCTTCTCCAGTCCCCGATCCTGATTTAGTCGCCCAAGAAGTTCTTACGTAAGTCCACCCCCACATCAATTTTATTACTTCTTCTACATTTATAATTAATTCACATTGTAGTTACTTTTTTTTCTTTTTAAATACTTTTTCAAAATTATATTTCGTTAATATTGAATGACAGTAAAACGTACCTTTTGTTTTCCTAACAAATATATTCAAATGTTAAATAAGTAAAGTTTAGAGAATTTGTTCATTTCACAATCAAGTTTATTTATCTCAATTTCTTACTATAATTTGAAAGAGATGTATAAAAATGTAGTTTTTGAACTTAATTTTCAAAAACTAAAAATTGAAAAGCTACCTTTGGATAGTCAAGATTTTAGGCCTAAATTTGTGTTGGATGTTTATGTTTTATGGGTGATGGATTTGCAGGAGCATCAACAATGCAACAATAACAAGAAGGAATTTGGGGTTCCTATCCTGTAAAACTGGCAACCCGATAGACGACTGCTGGCGTTGCGATGCCAATTGGGAAAAGAACCGAAAGAAGCTAGCCGACTGCGCCATTGGGTTTGGAAAACGAGCCATCGGCGGCAAAAACGGCAAATACTACATCGTGACTGACCCATCCGACAATGACGTGGTGAACCCAAAACCGGGCACACTCCGGCACGCCGTAATCCAAAACGAACCCTTATGGATCACCTTCAAACGCGACATGGTCATCAAATTAAAAGCAGAGCTTCTGATGAACTCTTTCAAAACCATCGACGGGCGTGGCGTCTCCGTCCACATCGCCGGAGGGCCTTGCATTACCATTCAATTTGTGACCAACATTATAATCCACGGCATTAACATTCACGATTGCAAGCAAGGTGGGAACACTTACGTGAGAGACTCGCCGGAGCATTACGGTTGGAGGACGGTGTCAGACGGGGATGGGATATCTATATTTGGAGGAAGCCATGTTTGGATTGACCATTGCTCTTTGTCGAATTGCCGAGATGGGTTGATTGATGCGATTCATGGATCGACGGCTATTACTGTTTCAAACAATTACATGACTCATCATAACAAAGTTATGCTTTTAGGACACAGTGATTCCTATACTCAAGATAAGAACATGCAAGTCACCATTGCTTTTAACCATTTCGGTGAAGGCCTTGTTCAAAGAATGCCACGGTAAGTTCTATTTTTATAATCAAAGTTTGAGAATGTAAGGGTAGCTATACTTTGTTGAGAAGTCAACGAGTCCTCTAAATCAATTTGACATTTTTTGTATTGATGGACAGGTGTCGCCACGGATATTTCCATGTGGTAAATAATGACTATACTCATTGGATAATGTATGCCATTGGTGGAAGTGCATCTCCTACTATCAATAGCCAAGGAAATAGATACCTTGCTCCTAATAACCAAAATAGCAAAGAGGTGAGAGAATTGAATCAGTTAAGTTAAGAAGTTATCGTTTCCATTCAAATAGGTTATATTTGTTAAATTAACTTAAAGTTGATGGTTGGCCTTTTAGGTGACAAAACGAGAGGATACACCCCAAAGCAGGTGGAAGAATTGGAATTGGAGATCAGATGGAGATTTGATGTTGAATGGAGCATTCTTCGTAAGGTCCGGGGCTGGAGCATCGTCTAGCTATGCAAGAGCATCCAGCTTGAGTGCTAAGTCCTCGTCACTAATCAATTCGATCACGGCAGGAGCTGGAGTGTTGAAATGTCGAAAAGGGTCTCGATGCTAGTCCAACCACTGTTGGTGATATATATACATATTATGAACCAATATGACATTATATTAAAAGATAAATCAGCAAGGAGAGATTTGAGAATCTTCTTGTATAGTATAATTTTAATTTGATTGGTCACAACTAAAAGGCTTTAAGAAAAAGTCATATTTCAGAGTGAGTGACCCAACATTTGTAATTCCGTCTAATTACAATATTAAGATAAATATTATTATGC

mRNA sequence

CCAAACTCTCCCAAATCTCAATCAATCCTTTTCTCTGCTAATATTCACACAAGAAAGAAAGAAAGAAAGAAAGAGAGAGAGAGAGAGAGCTATGGAAAATTCCAATTCTGGTGTGATTATGATGAGTATAAAGTTATTAGTATTGAGTATTGTTATGCTCCAAATGAGCTCCATGTCATCGTCTTCTCCAGTCCCCGATCCTGATTTAGTCGCCCAAGAAGTTCTTACGAGCATCAACAATGCAACAATAACAAGAAGGAATTTGGGGTTCCTATCCTGTAAAACTGGCAACCCGATAGACGACTGCTGGCGTTGCGATGCCAATTGGGAAAAGAACCGAAAGAAGCTAGCCGACTGCGCCATTGGGTTTGGAAAACGAGCCATCGGCGGCAAAAACGGCAAATACTACATCGTGACTGACCCATCCGACAATGACGTGGTGAACCCAAAACCGGGCACACTCCGGCACGCCGTAATCCAAAACGAACCCTTATGGATCACCTTCAAACGCGACATGGTCATCAAATTAAAAGCAGAGCTTCTGATGAACTCTTTCAAAACCATCGACGGGCGTGGCGTCTCCGTCCACATCGCCGGAGGGCCTTGCATTACCATTCAATTTGTGACCAACATTATAATCCACGGCATTAACATTCACGATTGCAAGCAAGGTGGGAACACTTACGTGAGAGACTCGCCGGAGCATTACGGTTGGAGGACGGTGTCAGACGGGGATGGGATATCTATATTTGGAGGAAGCCATGTTTGGATTGACCATTGCTCTTTGTCGAATTGCCGAGATGGGTTGATTGATGCGATTCATGGATCGACGGCTATTACTGTTTCAAACAATTACATGACTCATCATAACAAAGTTATGCTTTTAGGACACAGTGATTCCTATACTCAAGATAAGAACATGCAAGTCACCATTGCTTTTAACCATTTCGGTGAAGGCCTTGTTCAAAGAATGCCACGGTGTCGCCACGGATATTTCCATGTGGTAAATAATGACTATACTCATTGGATAATGTATGCCATTGGTGGAAGTGCATCTCCTACTATCAATAGCCAAGGAAATAGATACCTTGCTCCTAATAACCAAAATAGCAAAGAGGTGACAAAACGAGAGGATACACCCCAAAGCAGGTGGAAGAATTGGAATTGGAGATCAGATGGAGATTTGATGTTGAATGGAGCATTCTTCGTAAGGTCCGGGGCTGGAGCATCGTCTAGCTATGCAAGAGCATCCAGCTTGAGTGCTAAGTCCTCGTCACTAATCAATTCGATCACGGCAGGAGCTGGAGTGTTGAAATGTCGAAAAGGGTCTCGATGCTAGTCCAACCACTGTTGGTGATATATATACATATTATGAACCAATATGACATTATATTAAAAGATAAATCAGCAAGGAGAGATTTGAGAATCTTCTTGTATAGTATAATTTTAATTTGATTGGTCACAACTAAAAGGCTTTAAGAAAAAGTCATATTTCAGAGTGAGTGACCCAACATTTGTAATTCCGTCTAATTACAATATTAAGATAAATATTATTATGC

Coding sequence (CDS)

ATGGAAAATTCCAATTCTGGTGTGATTATGATGAGTATAAAGTTATTAGTATTGAGTATTGTTATGCTCCAAATGAGCTCCATGTCATCGTCTTCTCCAGTCCCCGATCCTGATTTAGTCGCCCAAGAAGTTCTTACGAGCATCAACAATGCAACAATAACAAGAAGGAATTTGGGGTTCCTATCCTGTAAAACTGGCAACCCGATAGACGACTGCTGGCGTTGCGATGCCAATTGGGAAAAGAACCGAAAGAAGCTAGCCGACTGCGCCATTGGGTTTGGAAAACGAGCCATCGGCGGCAAAAACGGCAAATACTACATCGTGACTGACCCATCCGACAATGACGTGGTGAACCCAAAACCGGGCACACTCCGGCACGCCGTAATCCAAAACGAACCCTTATGGATCACCTTCAAACGCGACATGGTCATCAAATTAAAAGCAGAGCTTCTGATGAACTCTTTCAAAACCATCGACGGGCGTGGCGTCTCCGTCCACATCGCCGGAGGGCCTTGCATTACCATTCAATTTGTGACCAACATTATAATCCACGGCATTAACATTCACGATTGCAAGCAAGGTGGGAACACTTACGTGAGAGACTCGCCGGAGCATTACGGTTGGAGGACGGTGTCAGACGGGGATGGGATATCTATATTTGGAGGAAGCCATGTTTGGATTGACCATTGCTCTTTGTCGAATTGCCGAGATGGGTTGATTGATGCGATTCATGGATCGACGGCTATTACTGTTTCAAACAATTACATGACTCATCATAACAAAGTTATGCTTTTAGGACACAGTGATTCCTATACTCAAGATAAGAACATGCAAGTCACCATTGCTTTTAACCATTTCGGTGAAGGCCTTGTTCAAAGAATGCCACGGTGTCGCCACGGATATTTCCATGTGGTAAATAATGACTATACTCATTGGATAATGTATGCCATTGGTGGAAGTGCATCTCCTACTATCAATAGCCAAGGAAATAGATACCTTGCTCCTAATAACCAAAATAGCAAAGAGGTGACAAAACGAGAGGATACACCCCAAAGCAGGTGGAAGAATTGGAATTGGAGATCAGATGGAGATTTGATGTTGAATGGAGCATTCTTCGTAAGGTCCGGGGCTGGAGCATCGTCTAGCTATGCAAGAGCATCCAGCTTGAGTGCTAAGTCCTCGTCACTAATCAATTCGATCACGGCAGGAGCTGGAGTGTTGAAATGTCGAAAAGGGTCTCGATGCTAG

Protein sequence

MENSNSGVIMMSIKLLVLSIVMLQMSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGFLSCKTGNPIDDCWRCDANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPKPGTLRHAVIQNEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWRSDGDLMLNGAFFVRSGAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC*
Homology
BLAST of CSPI02G17490 vs. ExPASy Swiss-Prot
Match: Q9FXD8 (Probable pectate lyase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g67750 PE=2 SV=2)

HSP 1 Score: 658.7 bits (1698), Expect = 4.4e-188
Identity = 313/407 (76.90%), Postives = 354/407 (86.98%), Query Frame = 0

Query: 10  MMSIKLLVLSIVMLQMS-SMSSSSPVPDPDLVAQEVLTSINNATITRRNLGFLSCKTGNP 69
           ++ + L + S ++L +S +  +S+PV +P+LV QEV   IN    +RRNLG LSC TGNP
Sbjct: 5   LVHLSLSLFSCLLLVLSPTFIASTPVSEPELVVQEVNEKIN---ASRRNLGVLSCGTGNP 64

Query: 70  IDDCWRCDANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPKPGTLRHAV 129
           IDDCWRCD  WEKNR++LADCAIGFGK AIGG++GK Y+VTD SD DVVNPKPGTLRHAV
Sbjct: 65  IDDCWRCDPKWEKNRQRLADCAIGFGKHAIGGRDGKIYVVTDSSDKDVVNPKPGTLRHAV 124

Query: 130 IQNEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINI 189
           IQ+EPLWI F RDMVIKLK EL+MNSFKTIDGRG SVHIAGG CIT+Q+VTNIIIHG+NI
Sbjct: 125 IQDEPLWIIFARDMVIKLKEELIMNSFKTIDGRGASVHIAGGACITVQYVTNIIIHGVNI 184

Query: 190 HDCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTA 249
           HDCK+ GN YVRDSP HYGWRT SDGD +SIFGGSHVW+DHCSLSNC DGLIDAIHGSTA
Sbjct: 185 HDCKRKGNAYVRDSPSHYGWRTASDGDAVSIFGGSHVWVDHCSLSNCADGLIDAIHGSTA 244

Query: 250 ITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 309
           IT+SNNY++HHNKVMLLGHSDSYT+DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND
Sbjct: 245 ITISNNYLSHHNKVMLLGHSDSYTRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 304

Query: 310 YTHWIMYAIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWRSDGDLMLN 369
           YTHW MYAIGGSA+PTINSQGNR+LAPN+   KEVTK ED P+S+WK WNWRS+GDL LN
Sbjct: 305 YTHWQMYAIGGSAAPTINSQGNRFLAPNDHVFKEVTKYEDAPRSKWKKWNWRSEGDLFLN 364

Query: 370 GAFFVRSGAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC 416
           GAFF  SG GASSSYA+ASSLSA+ SSL+ S+T+ AG L CRKGSRC
Sbjct: 365 GAFFTPSGGGASSSYAKASSLSARPSSLVASVTSNAGALFCRKGSRC 408

BLAST of CSPI02G17490 vs. ExPASy Swiss-Prot
Match: Q93Z25 (Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 1.0e-184
Identity = 299/392 (76.28%), Postives = 348/392 (88.78%), Query Frame = 0

Query: 26  SSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGFLSCKTGNPIDDCWRCDANWEKNRKK 85
           S+++SS PV DP+LV +EV   IN  +I+RR LGF SC +GNPIDDCWRCD +WEKNRK+
Sbjct: 42  SALASSLPVSDPELVVEEVHRKINE-SISRRKLGFFSCGSGNPIDDCWRCDKDWEKNRKR 101

Query: 86  LADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPKPGTLRHAVIQNEPLWITFKRDMVIK 145
           LADC IGFGK AIGG++G+ Y+VTDP ++D VNP+PGTLR+AVIQ+EPLWI FKRDM I+
Sbjct: 102 LADCGIGFGKNAIGGRDGEIYVVTDPGNDDPVNPRPGTLRYAVIQDEPLWIIFKRDMTIQ 161

Query: 146 LKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNTYVRDSPEH 205
           LK EL+MNSFKT+DGRG SVHI+GGPCITIQ+VTNIIIHG++IHDCKQGGNTYVRDSPEH
Sbjct: 162 LKEELIMNSFKTLDGRGASVHISGGPCITIQYVTNIIIHGLHIHDCKQGGNTYVRDSPEH 221

Query: 206 YGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNYMTHHNKVMLL 265
           YG+RTVSDGDG+SIFGGSHVW+DHCSLSNC DGLIDAI GSTAIT+SNNY+THHNKVMLL
Sbjct: 222 YGYRTVSDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIRGSTAITISNNYLTHHNKVMLL 281

Query: 266 GHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAIGGSASPTI 325
           GHSD+Y QDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW MYAIGGSA+PTI
Sbjct: 282 GHSDTYEQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTI 341

Query: 326 NSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWRSDGDLMLNGAFFVRSGAG--ASSSY 385
           NSQGNR+LAP++ +SKEVTK ED P+  W+NWNWRS+GDL+LNGAFF  SGAG   SSSY
Sbjct: 342 NSQGNRFLAPDDSSSKEVTKHEDAPEDEWRNWNWRSEGDLLLNGAFFTYSGAGPAKSSSY 401

Query: 386 ARASSLSAKSSSLINSITAGAGVLKCRKGSRC 416
           ++ASSL+A+ SS +  IT  +G L C++GS C
Sbjct: 402 SKASSLAARPSSHVGEITIASGALSCKRGSHC 432

BLAST of CSPI02G17490 vs. ExPASy Swiss-Prot
Match: Q9C5M8 (Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2)

HSP 1 Score: 639.8 bits (1649), Expect = 2.1e-182
Identity = 298/406 (73.40%), Postives = 348/406 (85.71%), Query Frame = 0

Query: 10  MMSIKLLVLSIVMLQMSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGFLSCKTGNPI 69
           M + KL +  +  L  + +  SSPVPDP+ V +EV  SIN +   RR LG+LSC TGNPI
Sbjct: 3   MQTKKLFITIVSFLLYAPLFLSSPVPDPESVVEEVHKSINASVAGRRKLGYLSCTTGNPI 62

Query: 70  DDCWRCDANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPKPGTLRHAVI 129
           DDCWRCD +WE++R++LADCAIGFGK AIGG++G+ Y+VTD  +++ V+PKPGTLRHAV+
Sbjct: 63  DDCWRCDPHWEQHRQRLADCAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVV 122

Query: 130 QNEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIH 189
           Q+EPLWI F+RDM I+LK EL+MNSFKTIDGRG SVHI+GGPCITIQ+VTNIIIHGI+IH
Sbjct: 123 QDEPLWIIFQRDMTIQLKEELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIH 182

Query: 190 DCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAI 249
           DCKQGGN  VR SP H+GWRT+SDGDG+SIFGGSHVW+DHCS SNC DGLIDAI GSTAI
Sbjct: 183 DCKQGGNAMVRSSPRHFGWRTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAI 242

Query: 250 TVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDY 309
           T+SNN+MTHH+KVMLLGHSD+Y++DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDY
Sbjct: 243 TLSNNHMTHHDKVMLLGHSDTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDY 302

Query: 310 THWIMYAIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWRSDGDLMLNG 369
           THW MYAIGGSA+PTINSQGNR+LAPN + SKEVTK ED P+S WK WNWRS GDL+LNG
Sbjct: 303 THWEMYAIGGSANPTINSQGNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNG 362

Query: 370 AFFVRSGAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC 416
           AFF  SG  ASSSYA+ASSL AK SSL+  +T+ +G L CRKGSRC
Sbjct: 363 AFFTPSGGAASSSYAKASSLGAKPSSLVGPLTSTSGALNCRKGSRC 408

BLAST of CSPI02G17490 vs. ExPASy Swiss-Prot
Match: O24554 (Pectate lyase OS=Zinnia violacea OX=34245 PE=1 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 5.3e-173
Identity = 289/396 (72.98%), Postives = 331/396 (83.59%), Query Frame = 0

Query: 20  IVMLQMSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGFLSCKTGNPIDDCWRCDANW 79
           I+ +   +++SSSP   P  +  EV  SIN    +RRNLG+LSC TGNPIDDCWRCD NW
Sbjct: 9   ILFISSLAIASSSPSRTPHAIVNEVHKSIN---ASRRNLGYLSCGTGNPIDDCWRCDPNW 68

Query: 80  EKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPKPGTLRHAVIQNEPLWITFK 139
             NR++LADCAIGFGK A+GG+NG+ Y+VTDP ++D VNP PGTLR+AVIQ+EPLWI FK
Sbjct: 69  ANNRQRLADCAIGFGKNAMGGRNGRIYVVTDPGNDDPVNPVPGTLRYAVIQDEPLWIIFK 128

Query: 140 RDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNTYV 199
           RDMVI+L+ EL+MNS KTIDGRGV+VHI  GPCITI + +NIIIHGI+IHDCKQ GN  +
Sbjct: 129 RDMVIQLRQELVMNSHKTIDGRGVNVHIGNGPCITIHYASNIIIHGIHIHDCKQAGNGNI 188

Query: 200 RDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNYMTHH 259
           R+SP H GW T SDGDGISIF    +WIDH SLSNC DGLIDAIHGSTAIT+SNNYMTHH
Sbjct: 189 RNSPHHSGWWTQSDGDGISIFASKDIWIDHNSLSNCHDGLIDAIHGSTAITISNNYMTHH 248

Query: 260 NKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAIGG 319
           +KVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW MYAIGG
Sbjct: 249 DKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGG 308

Query: 320 SASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWRSDGDLMLNGAFFVRSGAGA 379
           SASPTI SQGNR+LAPN +  KEVTK E+ P+S WKNWNWRS+GDLMLNGA+F  SG  A
Sbjct: 309 SASPTIYSQGNRFLAPNTRFDKEVTKHENAPESEWKNWNWRSEGDLMLNGAYFRESGGRA 368

Query: 380 SSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC 416
           +SS+ARASSLS + S+L+ S+T  AG L CRKGSRC
Sbjct: 369 ASSFARASSLSGRPSTLVASMTRSAGALVCRKGSRC 401

BLAST of CSPI02G17490 vs. ExPASy Swiss-Prot
Match: Q9LTZ0 (Putative pectate lyase 11 OS=Arabidopsis thaliana OX=3702 GN=At3g27400 PE=3 SV=2)

HSP 1 Score: 606.7 bits (1563), Expect = 2.0e-172
Identity = 287/401 (71.57%), Postives = 333/401 (83.04%), Query Frame = 0

Query: 15  LLVLSIVMLQMSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGFLSCKTGNPIDDCWR 74
           LL+L+I      S S    V DP+LV  +V  S+ NA  +RR+L +LSC+TGNPIDDCWR
Sbjct: 14  LLLLTIGNTLAFSSSLPDHVQDPNLVVDDVNRSVFNA--SRRSLAYLSCRTGNPIDDCWR 73

Query: 75  CDANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPKPGTLRHAVIQNEPL 134
           CD NWE NR++LADCAIGFGK AIGG+ G+ Y+VTDP+++D VNP+PGTLR+AV Q EPL
Sbjct: 74  CDPNWETNRQRLADCAIGFGKNAIGGRKGRIYVVTDPANDDPVNPRPGTLRYAVTQEEPL 133

Query: 135 WITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQG 194
           WI FKRDMVI+LK EL++ SFKTIDGRG SVHI  GPC+ I + TNIIIHGINIHDCK G
Sbjct: 134 WIIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGPCLKIHYATNIIIHGINIHDCKPG 193

Query: 195 GNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNN 254
               ++D P H GW   SDGD ++IFGG HVWIDHCSLSNC DGLIDAIHGSTAIT+SNN
Sbjct: 194 SGGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCSLSNCDDGLIDAIHGSTAITISNN 253

Query: 255 YMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIM 314
           +MTHH+KVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW M
Sbjct: 254 HMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 313

Query: 315 YAIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWRSDGDLMLNGAFFVR 374
           YAIGGSASPTI SQGNR+LAPN + +KEVTK ED P+S+W++WNWRS+GD++LNGA+F  
Sbjct: 314 YAIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDAPESKWRDWNWRSEGDMLLNGAYFRE 373

Query: 375 SGAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC 416
           SGA A S+YARASSLSA+ SSL+ SIT  AG L CR+G RC
Sbjct: 374 SGAEAPSTYARASSLSARPSSLVGSITTTAGTLSCRRGRRC 412

BLAST of CSPI02G17490 vs. ExPASy TrEMBL
Match: A0A0A0LKL0 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_2G350210 PE=3 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 4.7e-241
Identity = 413/415 (99.52%), Postives = 413/415 (99.52%), Query Frame = 0

Query: 1   MENSNSGVIMMSIKLLVLSIVMLQMSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGF 60
           MENSNS VIMMSIKLLVLSIVMLQMSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGF
Sbjct: 1   MENSNSVVIMMSIKLLVLSIVMLQMSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGF 60

Query: 61  LSCKTGNPIDDCWRCDANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPK 120
           LSCKTGNPIDDCWRCDANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPK
Sbjct: 61  LSCKTGNPIDDCWRCDANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPK 120

Query: 121 PGTLRHAVIQNEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTN 180
           PGTLRHAVIQ EPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTN
Sbjct: 121 PGTLRHAVIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTN 180

Query: 181 IIIHGINIHDCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLI 240
           IIIHGINIHDCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLI
Sbjct: 181 IIIHGINIHDCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLI 240

Query: 241 DAIHGSTAITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHG 300
           DAIHGSTAITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHG
Sbjct: 241 DAIHGSTAITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHG 300

Query: 301 YFHVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWR 360
           YFHVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWR
Sbjct: 301 YFHVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWR 360

Query: 361 SDGDLMLNGAFFVRSGAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC 416
           SDGDLMLNGAFFVRSGAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC
Sbjct: 361 SDGDLMLNGAFFVRSGAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC 415

BLAST of CSPI02G17490 vs. ExPASy TrEMBL
Match: A0A1S3BB18 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103487738 PE=3 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 3.9e-227
Identity = 390/416 (93.75%), Postives = 403/416 (96.88%), Query Frame = 0

Query: 1   MENSNSGVIM-MSIKLLVLSIVMLQMSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLG 60
           MEN    VIM +SIK+LV S+VMLQM S+SSSSPVPDPDLVAQEVLTSINNAT TRRNLG
Sbjct: 1   MENPKFVVIMIISIKVLVFSMVMLQMCSISSSSPVPDPDLVAQEVLTSINNAT-TRRNLG 60

Query: 61  FLSCKTGNPIDDCWRCDANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNP 120
           FLSC+TGNPIDDCWRCDANWEKNRK+LA+CAIGFGKRAIGGKNG+YYIVTDPSDNDVVNP
Sbjct: 61  FLSCRTGNPIDDCWRCDANWEKNRKRLANCAIGFGKRAIGGKNGRYYIVTDPSDNDVVNP 120

Query: 121 KPGTLRHAVIQNEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVT 180
           KPGTLRHAVIQ EPLWI FKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVT
Sbjct: 121 KPGTLRHAVIQKEPLWIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVT 180

Query: 181 NIIIHGINIHDCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGL 240
           NIIIHGINIHDCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGL
Sbjct: 181 NIIIHGINIHDCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGL 240

Query: 241 IDAIHGSTAITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRH 300
           IDAIHGSTAITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRH
Sbjct: 241 IDAIHGSTAITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRH 300

Query: 301 GYFHVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNW 360
           GYFHVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNNQ+ KEVTKRED PQS+WKNWNW
Sbjct: 301 GYFHVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNW 360

Query: 361 RSDGDLMLNGAFFVRSGAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC 416
           RSDGDLMLNGAFFVRSGAGASS YARASSLSAKSSSL++SIT+GAGVLKCRKGSRC
Sbjct: 361 RSDGDLMLNGAFFVRSGAGASSRYARASSLSAKSSSLVSSITSGAGVLKCRKGSRC 415

BLAST of CSPI02G17490 vs. ExPASy TrEMBL
Match: A0A5A7V5E9 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001810 PE=3 SV=1)

HSP 1 Score: 780.8 bits (2015), Expect = 2.9e-222
Identity = 375/391 (95.91%), Postives = 384/391 (98.21%), Query Frame = 0

Query: 25  MSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGFLSCKTGNPIDDCWRCDANWEKNRK 84
           M S+SSSSPVPDPDLVAQEVLTSINNAT TRRNLGFLSC+TGNPIDDCWRCDANWEKNRK
Sbjct: 1   MCSISSSSPVPDPDLVAQEVLTSINNAT-TRRNLGFLSCRTGNPIDDCWRCDANWEKNRK 60

Query: 85  KLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPKPGTLRHAVIQNEPLWITFKRDMVI 144
           +LADCAIGFGKRAIGGKNG+YYIVTDPSDNDVVNPKPGTLRHAVIQ EPLWI FKRDMVI
Sbjct: 61  RLADCAIGFGKRAIGGKNGRYYIVTDPSDNDVVNPKPGTLRHAVIQKEPLWIIFKRDMVI 120

Query: 145 KLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNTYVRDSPE 204
           KLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNTYVRDSPE
Sbjct: 121 KLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNTYVRDSPE 180

Query: 205 HYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNYMTHHNKVML 264
           HYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNYMTHHNKVML
Sbjct: 181 HYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNYMTHHNKVML 240

Query: 265 LGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAIGGSASPT 324
           LGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAIGGSASPT
Sbjct: 241 LGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAIGGSASPT 300

Query: 325 INSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWRSDGDLMLNGAFFVRSGAGASSSYA 384
           INSQGNRYLAPNNQ+ KEVTKRED PQS+WKNWNWRSDGDLMLNGAFFVRSGAGASS YA
Sbjct: 301 INSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLNGAFFVRSGAGASSRYA 360

Query: 385 RASSLSAKSSSLINSITAGAGVLKCRKGSRC 416
           RASSLSAKSSSL++SIT+GAGVLKCRKGSRC
Sbjct: 361 RASSLSAKSSSLVSSITSGAGVLKCRKGSRC 390

BLAST of CSPI02G17490 vs. ExPASy TrEMBL
Match: A0A6J1K9C7 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111491836 PE=3 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 1.7e-214
Identity = 361/400 (90.25%), Postives = 383/400 (95.75%), Query Frame = 0

Query: 16  LVLSIVMLQMSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGFLSCKTGNPIDDCWRC 75
           ++   ++LQM S+ SSSPV DP+LV QEVL+SINNAT TRRNLGFLSC TGNPIDDCWRC
Sbjct: 9   IITFTLLLQMCSI-SSSPVADPELVVQEVLSSINNAT-TRRNLGFLSCGTGNPIDDCWRC 68

Query: 76  DANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPKPGTLRHAVIQNEPLW 135
           D NWEKNRK+LADC IGFGKRAIGGKNGK+Y+VTDPSD DVVNPKPGTLRHAVIQ EPLW
Sbjct: 69  DPNWEKNRKRLADCGIGFGKRAIGGKNGKFYVVTDPSDKDVVNPKPGTLRHAVIQKEPLW 128

Query: 136 ITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGG 195
           ITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQ+VTNIIIHGINIHDCKQGG
Sbjct: 129 ITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQYVTNIIIHGINIHDCKQGG 188

Query: 196 NTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNY 255
           NTYVRDSPEHYGWRT+SDGDG+SIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNY
Sbjct: 189 NTYVRDSPEHYGWRTLSDGDGVSIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNY 248

Query: 256 MTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMY 315
           MTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMY
Sbjct: 249 MTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMY 308

Query: 316 AIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWRSDGDLMLNGAFFVRS 375
           AIGGSASPTINSQGNRYLAPNN++ KEVTKRED PQS+WKNWNWRS GDLMLNGAFF RS
Sbjct: 309 AIGGSASPTINSQGNRYLAPNNRDRKEVTKREDAPQSKWKNWNWRSSGDLMLNGAFFTRS 368

Query: 376 GAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC 416
           GAG+SSSYARASSLSAKSSSL++++TAGAGVL+CRKGSRC
Sbjct: 369 GAGSSSSYARASSLSAKSSSLVSTMTAGAGVLRCRKGSRC 406

BLAST of CSPI02G17490 vs. ExPASy TrEMBL
Match: A0A6J1HD44 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111462874 PE=3 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 6.4e-214
Identity = 360/400 (90.00%), Postives = 382/400 (95.50%), Query Frame = 0

Query: 16  LVLSIVMLQMSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGFLSCKTGNPIDDCWRC 75
           ++   ++LQM ++ SSSPV DP LV QEVL+SINNAT TRRNLGFLSC TGNPIDDCWRC
Sbjct: 9   IITFTLLLQMCAI-SSSPVADPQLVVQEVLSSINNAT-TRRNLGFLSCGTGNPIDDCWRC 68

Query: 76  DANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPKPGTLRHAVIQNEPLW 135
           D NWEKNRK+LADC IGFGKRAIGGKNGK+Y+VTDPSD DVVNPKPGTLRHAVIQ EPLW
Sbjct: 69  DPNWEKNRKRLADCGIGFGKRAIGGKNGKFYVVTDPSDKDVVNPKPGTLRHAVIQKEPLW 128

Query: 136 ITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGG 195
           ITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQ+VTNIIIHGINIHDCKQGG
Sbjct: 129 ITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQYVTNIIIHGINIHDCKQGG 188

Query: 196 NTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNY 255
           NTYVRDSPEHYGWRT+SDGDG+SIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNY
Sbjct: 189 NTYVRDSPEHYGWRTLSDGDGVSIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNY 248

Query: 256 MTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMY 315
           MTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMY
Sbjct: 249 MTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMY 308

Query: 316 AIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWRSDGDLMLNGAFFVRS 375
           AIGGSASPTINSQGNRYLAPNN++ KEVTKRED PQS+WKNWNWRS GDLMLNGAFF RS
Sbjct: 309 AIGGSASPTINSQGNRYLAPNNRDRKEVTKREDAPQSKWKNWNWRSSGDLMLNGAFFTRS 368

Query: 376 GAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC 416
           GAG+SSSYARASSLSAKSSSL++++TAGAGVL+CRKGSRC
Sbjct: 369 GAGSSSSYARASSLSAKSSSLVSTMTAGAGVLRCRKGSRC 406

BLAST of CSPI02G17490 vs. NCBI nr
Match: XP_004143054.1 (probable pectate lyase 5 [Cucumis sativus] >KGN62338.1 hypothetical protein Csa_018558 [Cucumis sativus])

HSP 1 Score: 843.2 bits (2177), Expect = 9.7e-241
Identity = 413/415 (99.52%), Postives = 413/415 (99.52%), Query Frame = 0

Query: 1   MENSNSGVIMMSIKLLVLSIVMLQMSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGF 60
           MENSNS VIMMSIKLLVLSIVMLQMSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGF
Sbjct: 1   MENSNSVVIMMSIKLLVLSIVMLQMSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGF 60

Query: 61  LSCKTGNPIDDCWRCDANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPK 120
           LSCKTGNPIDDCWRCDANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPK
Sbjct: 61  LSCKTGNPIDDCWRCDANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPK 120

Query: 121 PGTLRHAVIQNEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTN 180
           PGTLRHAVIQ EPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTN
Sbjct: 121 PGTLRHAVIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTN 180

Query: 181 IIIHGINIHDCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLI 240
           IIIHGINIHDCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLI
Sbjct: 181 IIIHGINIHDCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLI 240

Query: 241 DAIHGSTAITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHG 300
           DAIHGSTAITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHG
Sbjct: 241 DAIHGSTAITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHG 300

Query: 301 YFHVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWR 360
           YFHVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWR
Sbjct: 301 YFHVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWR 360

Query: 361 SDGDLMLNGAFFVRSGAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC 416
           SDGDLMLNGAFFVRSGAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC
Sbjct: 361 SDGDLMLNGAFFVRSGAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC 415

BLAST of CSPI02G17490 vs. NCBI nr
Match: XP_008444399.1 (PREDICTED: probable pectate lyase 5 [Cucumis melo])

HSP 1 Score: 797.0 bits (2057), Expect = 8.0e-227
Identity = 390/416 (93.75%), Postives = 403/416 (96.88%), Query Frame = 0

Query: 1   MENSNSGVIM-MSIKLLVLSIVMLQMSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLG 60
           MEN    VIM +SIK+LV S+VMLQM S+SSSSPVPDPDLVAQEVLTSINNAT TRRNLG
Sbjct: 1   MENPKFVVIMIISIKVLVFSMVMLQMCSISSSSPVPDPDLVAQEVLTSINNAT-TRRNLG 60

Query: 61  FLSCKTGNPIDDCWRCDANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNP 120
           FLSC+TGNPIDDCWRCDANWEKNRK+LA+CAIGFGKRAIGGKNG+YYIVTDPSDNDVVNP
Sbjct: 61  FLSCRTGNPIDDCWRCDANWEKNRKRLANCAIGFGKRAIGGKNGRYYIVTDPSDNDVVNP 120

Query: 121 KPGTLRHAVIQNEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVT 180
           KPGTLRHAVIQ EPLWI FKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVT
Sbjct: 121 KPGTLRHAVIQKEPLWIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVT 180

Query: 181 NIIIHGINIHDCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGL 240
           NIIIHGINIHDCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGL
Sbjct: 181 NIIIHGINIHDCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGL 240

Query: 241 IDAIHGSTAITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRH 300
           IDAIHGSTAITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRH
Sbjct: 241 IDAIHGSTAITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRH 300

Query: 301 GYFHVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNW 360
           GYFHVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNNQ+ KEVTKRED PQS+WKNWNW
Sbjct: 301 GYFHVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNW 360

Query: 361 RSDGDLMLNGAFFVRSGAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC 416
           RSDGDLMLNGAFFVRSGAGASS YARASSLSAKSSSL++SIT+GAGVLKCRKGSRC
Sbjct: 361 RSDGDLMLNGAFFVRSGAGASSRYARASSLSAKSSSLVSSITSGAGVLKCRKGSRC 415

BLAST of CSPI02G17490 vs. NCBI nr
Match: KAA0061045.1 (putative pectate lyase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 780.8 bits (2015), Expect = 5.9e-222
Identity = 375/391 (95.91%), Postives = 384/391 (98.21%), Query Frame = 0

Query: 25  MSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGFLSCKTGNPIDDCWRCDANWEKNRK 84
           M S+SSSSPVPDPDLVAQEVLTSINNAT TRRNLGFLSC+TGNPIDDCWRCDANWEKNRK
Sbjct: 1   MCSISSSSPVPDPDLVAQEVLTSINNAT-TRRNLGFLSCRTGNPIDDCWRCDANWEKNRK 60

Query: 85  KLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPKPGTLRHAVIQNEPLWITFKRDMVI 144
           +LADCAIGFGKRAIGGKNG+YYIVTDPSDNDVVNPKPGTLRHAVIQ EPLWI FKRDMVI
Sbjct: 61  RLADCAIGFGKRAIGGKNGRYYIVTDPSDNDVVNPKPGTLRHAVIQKEPLWIIFKRDMVI 120

Query: 145 KLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNTYVRDSPE 204
           KLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNTYVRDSPE
Sbjct: 121 KLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNTYVRDSPE 180

Query: 205 HYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNYMTHHNKVML 264
           HYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNYMTHHNKVML
Sbjct: 181 HYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNYMTHHNKVML 240

Query: 265 LGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAIGGSASPT 324
           LGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAIGGSASPT
Sbjct: 241 LGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAIGGSASPT 300

Query: 325 INSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWRSDGDLMLNGAFFVRSGAGASSSYA 384
           INSQGNRYLAPNNQ+ KEVTKRED PQS+WKNWNWRSDGDLMLNGAFFVRSGAGASS YA
Sbjct: 301 INSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLNGAFFVRSGAGASSRYA 360

Query: 385 RASSLSAKSSSLINSITAGAGVLKCRKGSRC 416
           RASSLSAKSSSL++SIT+GAGVLKCRKGSRC
Sbjct: 361 RASSLSAKSSSLVSSITSGAGVLKCRKGSRC 390

BLAST of CSPI02G17490 vs. NCBI nr
Match: XP_022996659.1 (probable pectate lyase 5 [Cucurbita maxima])

HSP 1 Score: 755.0 bits (1948), Expect = 3.5e-214
Identity = 361/400 (90.25%), Postives = 383/400 (95.75%), Query Frame = 0

Query: 16  LVLSIVMLQMSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGFLSCKTGNPIDDCWRC 75
           ++   ++LQM S+ SSSPV DP+LV QEVL+SINNAT TRRNLGFLSC TGNPIDDCWRC
Sbjct: 9   IITFTLLLQMCSI-SSSPVADPELVVQEVLSSINNAT-TRRNLGFLSCGTGNPIDDCWRC 68

Query: 76  DANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPKPGTLRHAVIQNEPLW 135
           D NWEKNRK+LADC IGFGKRAIGGKNGK+Y+VTDPSD DVVNPKPGTLRHAVIQ EPLW
Sbjct: 69  DPNWEKNRKRLADCGIGFGKRAIGGKNGKFYVVTDPSDKDVVNPKPGTLRHAVIQKEPLW 128

Query: 136 ITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGG 195
           ITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQ+VTNIIIHGINIHDCKQGG
Sbjct: 129 ITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQYVTNIIIHGINIHDCKQGG 188

Query: 196 NTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNY 255
           NTYVRDSPEHYGWRT+SDGDG+SIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNY
Sbjct: 189 NTYVRDSPEHYGWRTLSDGDGVSIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNY 248

Query: 256 MTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMY 315
           MTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMY
Sbjct: 249 MTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMY 308

Query: 316 AIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWRSDGDLMLNGAFFVRS 375
           AIGGSASPTINSQGNRYLAPNN++ KEVTKRED PQS+WKNWNWRS GDLMLNGAFF RS
Sbjct: 309 AIGGSASPTINSQGNRYLAPNNRDRKEVTKREDAPQSKWKNWNWRSSGDLMLNGAFFTRS 368

Query: 376 GAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC 416
           GAG+SSSYARASSLSAKSSSL++++TAGAGVL+CRKGSRC
Sbjct: 369 GAGSSSSYARASSLSAKSSSLVSTMTAGAGVLRCRKGSRC 406

BLAST of CSPI02G17490 vs. NCBI nr
Match: KAG6598251.1 (putative pectate lyase 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 753.8 bits (1945), Expect = 7.7e-214
Identity = 357/386 (92.49%), Postives = 374/386 (96.89%), Query Frame = 0

Query: 30  SSSPVPDPDLVAQEVLTSINNATITRRNLGFLSCKTGNPIDDCWRCDANWEKNRKKLADC 89
           SSSPV DP+LV QEVL+SINNAT TRRNLGFLSC TGNPIDDCWRCD NWEKNRK+LADC
Sbjct: 5   SSSPVADPELVVQEVLSSINNAT-TRRNLGFLSCGTGNPIDDCWRCDPNWEKNRKRLADC 64

Query: 90  AIGFGKRAIGGKNGKYYIVTDPSDNDVVNPKPGTLRHAVIQNEPLWITFKRDMVIKLKAE 149
            IGFGKRAIGGKNGK+Y+VTDPSD DVVNPKPGTLRHAVIQ EPLWITFKRDMVIKLKAE
Sbjct: 65  GIGFGKRAIGGKNGKFYVVTDPSDKDVVNPKPGTLRHAVIQKEPLWITFKRDMVIKLKAE 124

Query: 150 LLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNTYVRDSPEHYGWR 209
           LLMNSFKTIDGRGVSVHIAGGPCITIQ+VTNIIIHGINIHDCKQGGNTYVRDSPEHYGWR
Sbjct: 125 LLMNSFKTIDGRGVSVHIAGGPCITIQYVTNIIIHGINIHDCKQGGNTYVRDSPEHYGWR 184

Query: 210 TVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNYMTHHNKVMLLGHSD 269
           T+SDGDG+SIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNYMTHHNKVMLLGHSD
Sbjct: 185 TLSDGDGVSIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNYMTHHNKVMLLGHSD 244

Query: 270 SYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAIGGSASPTINSQG 329
           SYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAIGGSASPTINSQG
Sbjct: 245 SYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAIGGSASPTINSQG 304

Query: 330 NRYLAPNNQNSKEVTKREDTPQSRWKNWNWRSDGDLMLNGAFFVRSGAGASSSYARASSL 389
           NRYLAPNN++ KEVTKRED PQS+WKNWNWRS GDLMLNGAFF RSGAG+SSSYARASSL
Sbjct: 305 NRYLAPNNRDRKEVTKREDAPQSKWKNWNWRSSGDLMLNGAFFTRSGAGSSSSYARASSL 364

Query: 390 SAKSSSLINSITAGAGVLKCRKGSRC 416
           SAKSSSL++++TAGAGVL+CRKGSRC
Sbjct: 365 SAKSSSLVSTMTAGAGVLRCRKGSRC 389

BLAST of CSPI02G17490 vs. TAIR 10
Match: AT1G67750.1 (Pectate lyase family protein )

HSP 1 Score: 658.7 bits (1698), Expect = 3.2e-189
Identity = 313/407 (76.90%), Postives = 354/407 (86.98%), Query Frame = 0

Query: 10  MMSIKLLVLSIVMLQMS-SMSSSSPVPDPDLVAQEVLTSINNATITRRNLGFLSCKTGNP 69
           ++ + L + S ++L +S +  +S+PV +P+LV QEV   IN    +RRNLG LSC TGNP
Sbjct: 5   LVHLSLSLFSCLLLVLSPTFIASTPVSEPELVVQEVNEKIN---ASRRNLGVLSCGTGNP 64

Query: 70  IDDCWRCDANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPKPGTLRHAV 129
           IDDCWRCD  WEKNR++LADCAIGFGK AIGG++GK Y+VTD SD DVVNPKPGTLRHAV
Sbjct: 65  IDDCWRCDPKWEKNRQRLADCAIGFGKHAIGGRDGKIYVVTDSSDKDVVNPKPGTLRHAV 124

Query: 130 IQNEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINI 189
           IQ+EPLWI F RDMVIKLK EL+MNSFKTIDGRG SVHIAGG CIT+Q+VTNIIIHG+NI
Sbjct: 125 IQDEPLWIIFARDMVIKLKEELIMNSFKTIDGRGASVHIAGGACITVQYVTNIIIHGVNI 184

Query: 190 HDCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTA 249
           HDCK+ GN YVRDSP HYGWRT SDGD +SIFGGSHVW+DHCSLSNC DGLIDAIHGSTA
Sbjct: 185 HDCKRKGNAYVRDSPSHYGWRTASDGDAVSIFGGSHVWVDHCSLSNCADGLIDAIHGSTA 244

Query: 250 ITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 309
           IT+SNNY++HHNKVMLLGHSDSYT+DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND
Sbjct: 245 ITISNNYLSHHNKVMLLGHSDSYTRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 304

Query: 310 YTHWIMYAIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWRSDGDLMLN 369
           YTHW MYAIGGSA+PTINSQGNR+LAPN+   KEVTK ED P+S+WK WNWRS+GDL LN
Sbjct: 305 YTHWQMYAIGGSAAPTINSQGNRFLAPNDHVFKEVTKYEDAPRSKWKKWNWRSEGDLFLN 364

Query: 370 GAFFVRSGAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC 416
           GAFF  SG GASSSYA+ASSLSA+ SSL+ S+T+ AG L CRKGSRC
Sbjct: 365 GAFFTPSGGGASSSYAKASSLSARPSSLVASVTSNAGALFCRKGSRC 408

BLAST of CSPI02G17490 vs. TAIR 10
Match: AT5G63180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 647.5 bits (1669), Expect = 7.3e-186
Identity = 299/392 (76.28%), Postives = 348/392 (88.78%), Query Frame = 0

Query: 26  SSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGFLSCKTGNPIDDCWRCDANWEKNRKK 85
           S+++SS PV DP+LV +EV   IN  +I+RR LGF SC +GNPIDDCWRCD +WEKNRK+
Sbjct: 42  SALASSLPVSDPELVVEEVHRKINE-SISRRKLGFFSCGSGNPIDDCWRCDKDWEKNRKR 101

Query: 86  LADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPKPGTLRHAVIQNEPLWITFKRDMVIK 145
           LADC IGFGK AIGG++G+ Y+VTDP ++D VNP+PGTLR+AVIQ+EPLWI FKRDM I+
Sbjct: 102 LADCGIGFGKNAIGGRDGEIYVVTDPGNDDPVNPRPGTLRYAVIQDEPLWIIFKRDMTIQ 161

Query: 146 LKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNTYVRDSPEH 205
           LK EL+MNSFKT+DGRG SVHI+GGPCITIQ+VTNIIIHG++IHDCKQGGNTYVRDSPEH
Sbjct: 162 LKEELIMNSFKTLDGRGASVHISGGPCITIQYVTNIIIHGLHIHDCKQGGNTYVRDSPEH 221

Query: 206 YGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNYMTHHNKVMLL 265
           YG+RTVSDGDG+SIFGGSHVW+DHCSLSNC DGLIDAI GSTAIT+SNNY+THHNKVMLL
Sbjct: 222 YGYRTVSDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIRGSTAITISNNYLTHHNKVMLL 281

Query: 266 GHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAIGGSASPTI 325
           GHSD+Y QDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW MYAIGGSA+PTI
Sbjct: 282 GHSDTYEQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTI 341

Query: 326 NSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWRSDGDLMLNGAFFVRSGAG--ASSSY 385
           NSQGNR+LAP++ +SKEVTK ED P+  W+NWNWRS+GDL+LNGAFF  SGAG   SSSY
Sbjct: 342 NSQGNRFLAPDDSSSKEVTKHEDAPEDEWRNWNWRSEGDLLLNGAFFTYSGAGPAKSSSY 401

Query: 386 ARASSLSAKSSSLINSITAGAGVLKCRKGSRC 416
           ++ASSL+A+ SS +  IT  +G L C++GS C
Sbjct: 402 SKASSLAARPSSHVGEITIASGALSCKRGSHC 432

BLAST of CSPI02G17490 vs. TAIR 10
Match: AT4G24780.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 639.8 bits (1649), Expect = 1.5e-183
Identity = 298/406 (73.40%), Postives = 348/406 (85.71%), Query Frame = 0

Query: 10  MMSIKLLVLSIVMLQMSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGFLSCKTGNPI 69
           M + KL +  +  L  + +  SSPVPDP+ V +EV  SIN +   RR LG+LSC TGNPI
Sbjct: 3   MQTKKLFITIVSFLLYAPLFLSSPVPDPESVVEEVHKSINASVAGRRKLGYLSCTTGNPI 62

Query: 70  DDCWRCDANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPKPGTLRHAVI 129
           DDCWRCD +WE++R++LADCAIGFGK AIGG++G+ Y+VTD  +++ V+PKPGTLRHAV+
Sbjct: 63  DDCWRCDPHWEQHRQRLADCAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVV 122

Query: 130 QNEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIH 189
           Q+EPLWI F+RDM I+LK EL+MNSFKTIDGRG SVHI+GGPCITIQ+VTNIIIHGI+IH
Sbjct: 123 QDEPLWIIFQRDMTIQLKEELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIH 182

Query: 190 DCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAI 249
           DCKQGGN  VR SP H+GWRT+SDGDG+SIFGGSHVW+DHCS SNC DGLIDAI GSTAI
Sbjct: 183 DCKQGGNAMVRSSPRHFGWRTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAI 242

Query: 250 TVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDY 309
           T+SNN+MTHH+KVMLLGHSD+Y++DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDY
Sbjct: 243 TLSNNHMTHHDKVMLLGHSDTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDY 302

Query: 310 THWIMYAIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWRSDGDLMLNG 369
           THW MYAIGGSA+PTINSQGNR+LAPN + SKEVTK ED P+S WK WNWRS GDL+LNG
Sbjct: 303 THWEMYAIGGSANPTINSQGNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNG 362

Query: 370 AFFVRSGAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC 416
           AFF  SG  ASSSYA+ASSL AK SSL+  +T+ +G L CRKGSRC
Sbjct: 363 AFFTPSGGAASSSYAKASSLGAKPSSLVGPLTSTSGALNCRKGSRC 408

BLAST of CSPI02G17490 vs. TAIR 10
Match: AT4G24780.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 639.8 bits (1649), Expect = 1.5e-183
Identity = 298/406 (73.40%), Postives = 348/406 (85.71%), Query Frame = 0

Query: 10  MMSIKLLVLSIVMLQMSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGFLSCKTGNPI 69
           M + KL +  +  L  + +  SSPVPDP+ V +EV  SIN +   RR LG+LSC TGNPI
Sbjct: 3   MQTKKLFITIVSFLLYAPLFLSSPVPDPESVVEEVHKSINASVAGRRKLGYLSCTTGNPI 62

Query: 70  DDCWRCDANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPKPGTLRHAVI 129
           DDCWRCD +WE++R++LADCAIGFGK AIGG++G+ Y+VTD  +++ V+PKPGTLRHAV+
Sbjct: 63  DDCWRCDPHWEQHRQRLADCAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVV 122

Query: 130 QNEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIH 189
           Q+EPLWI F+RDM I+LK EL+MNSFKTIDGRG SVHI+GGPCITIQ+VTNIIIHGI+IH
Sbjct: 123 QDEPLWIIFQRDMTIQLKEELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIH 182

Query: 190 DCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAI 249
           DCKQGGN  VR SP H+GWRT+SDGDG+SIFGGSHVW+DHCS SNC DGLIDAI GSTAI
Sbjct: 183 DCKQGGNAMVRSSPRHFGWRTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAI 242

Query: 250 TVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDY 309
           T+SNN+MTHH+KVMLLGHSD+Y++DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDY
Sbjct: 243 TLSNNHMTHHDKVMLLGHSDTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDY 302

Query: 310 THWIMYAIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWRSDGDLMLNG 369
           THW MYAIGGSA+PTINSQGNR+LAPN + SKEVTK ED P+S WK WNWRS GDL+LNG
Sbjct: 303 THWEMYAIGGSANPTINSQGNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNG 362

Query: 370 AFFVRSGAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC 416
           AFF  SG  ASSSYA+ASSL AK SSL+  +T+ +G L CRKGSRC
Sbjct: 363 AFFTPSGGAASSSYAKASSLGAKPSSLVGPLTSTSGALNCRKGSRC 408

BLAST of CSPI02G17490 vs. TAIR 10
Match: AT3G27400.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 606.7 bits (1563), Expect = 1.4e-173
Identity = 287/401 (71.57%), Postives = 333/401 (83.04%), Query Frame = 0

Query: 15  LLVLSIVMLQMSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGFLSCKTGNPIDDCWR 74
           LL+L+I      S S    V DP+LV  +V  S+ NA  +RR+L +LSC+TGNPIDDCWR
Sbjct: 14  LLLLTIGNTLAFSSSLPDHVQDPNLVVDDVNRSVFNA--SRRSLAYLSCRTGNPIDDCWR 73

Query: 75  CDANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPKPGTLRHAVIQNEPL 134
           CD NWE NR++LADCAIGFGK AIGG+ G+ Y+VTDP+++D VNP+PGTLR+AV Q EPL
Sbjct: 74  CDPNWETNRQRLADCAIGFGKNAIGGRKGRIYVVTDPANDDPVNPRPGTLRYAVTQEEPL 133

Query: 135 WITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQG 194
           WI FKRDMVI+LK EL++ SFKTIDGRG SVHI  GPC+ I + TNIIIHGINIHDCK G
Sbjct: 134 WIIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGPCLKIHYATNIIIHGINIHDCKPG 193

Query: 195 GNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNN 254
               ++D P H GW   SDGD ++IFGG HVWIDHCSLSNC DGLIDAIHGSTAIT+SNN
Sbjct: 194 SGGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCSLSNCDDGLIDAIHGSTAITISNN 253

Query: 255 YMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIM 314
           +MTHH+KVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW M
Sbjct: 254 HMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 313

Query: 315 YAIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWRSDGDLMLNGAFFVR 374
           YAIGGSASPTI SQGNR+LAPN + +KEVTK ED P+S+W++WNWRS+GD++LNGA+F  
Sbjct: 314 YAIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDAPESKWRDWNWRSEGDMLLNGAYFRE 373

Query: 375 SGAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC 416
           SGA A S+YARASSLSA+ SSL+ SIT  AG L CR+G RC
Sbjct: 374 SGAEAPSTYARASSLSARPSSLVGSITTTAGTLSCRRGRRC 412

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FXD84.4e-18876.90Probable pectate lyase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g67750 PE=2 SV=2[more]
Q93Z251.0e-18476.28Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1[more]
Q9C5M82.1e-18273.40Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2[more]
O245545.3e-17372.98Pectate lyase OS=Zinnia violacea OX=34245 PE=1 SV=1[more]
Q9LTZ02.0e-17271.57Putative pectate lyase 11 OS=Arabidopsis thaliana OX=3702 GN=At3g27400 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0LKL04.7e-24199.52Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_2G350210 PE=3 SV=1[more]
A0A1S3BB183.9e-22793.75Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103487738 PE=3 SV=1[more]
A0A5A7V5E92.9e-22295.91Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001810... [more]
A0A6J1K9C71.7e-21490.25Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111491836 PE=3 SV=1[more]
A0A6J1HD446.4e-21490.00Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111462874 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004143054.19.7e-24199.52probable pectate lyase 5 [Cucumis sativus] >KGN62338.1 hypothetical protein Csa_... [more]
XP_008444399.18.0e-22793.75PREDICTED: probable pectate lyase 5 [Cucumis melo][more]
KAA0061045.15.9e-22295.91putative pectate lyase 5 [Cucumis melo var. makuwa][more]
XP_022996659.13.5e-21490.25probable pectate lyase 5 [Cucurbita maxima][more]
KAG6598251.17.7e-21492.49putative pectate lyase 5, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT1G67750.13.2e-18976.90Pectate lyase family protein [more]
AT5G63180.17.3e-18676.28Pectin lyase-like superfamily protein [more]
AT4G24780.11.5e-18373.40Pectin lyase-like superfamily protein [more]
AT4G24780.21.5e-18373.40Pectin lyase-like superfamily protein [more]
AT3G27400.11.4e-17371.57Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 213..234
score: 64.72
coord: 357..381
score: 53.9
coord: 113..138
score: 67.03
coord: 386..409
score: 34.92
coord: 293..312
score: 78.1
coord: 89..106
score: 60.58
coord: 149..165
score: 66.67
coord: 315..334
score: 74.52
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 141..338
e-value: 1.2E-104
score: 363.6
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 176..330
e-value: 1.0E-18
score: 67.7
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 66..408
e-value: 8.8E-118
score: 395.2
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 16..412
NoneNo IPR availablePANTHERPTHR31683:SF151PECTATE LYASEcoord: 16..412
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 56..410

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G17490.1CSPI02G17490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity