CSPI02G13250 (gene) Cucumber (PI 183967) v1

BLAST of CSPI02G13250 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 5.9e-158
Identity = 301/504 (59.72%), Postives = 387/504 (76.79%), Query Frame = 0

Query: 12  MLLGLMAILSSCGSCS---FHTELSLEEATLKDLQRAFYQNKLTSRQLVEFYLEQVRRFN 71
           +L+   A++ S GS S     +  S++EAT++D++ AF + +LTS+QLVE YLE + + N
Sbjct: 15  LLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLEAISKLN 74

Query: 72  PILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFA 131
           PIL  +IE NPDAL QA  AD ER   +   L  LHG+PVL+KD+I+TKDKLNTTAGSFA
Sbjct: 75  PILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNTTAGSFA 134

Query: 132 LLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGKNPYTM-GE 191
           LLGSVV RDAGVV +LR +GA+I GKASLSEW++FRS ++P GWSARG QGKNPY +   
Sbjct: 135 LLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSARGLQGKNPYVLSAN 194

Query: 192 PCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISSRQ 251
           P GSSSGSAISV AN+V VSLGTETDGSIL P++ NSVVGIKP+VGLTSRAGVVPIS RQ
Sbjct: 195 PSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVGLTSRAGVVPISLRQ 254

Query: 252 DTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKGKRIGIVR 311
           D++GPICRTVSDA ++L+AIVG D  D +T  AS++IP+GGY QFL   GLKGKR+GIV 
Sbjct: 255 DSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGKRLGIVM 314

Query: 312 EFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGS-SGEQIALLAEFKI 371
           +          ++   +  +KTL++ GAI+++NL I N+E I  G+ SGE+IALLAEFK+
Sbjct: 315 K----------HSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLAEFKM 374

Query: 372 SLNAYLKELVASPIRSLSDAIEFNKKNSELENL-EYGQEEFLKAEATNGIGDAEKAALAR 431
           SLNAYLKELV SP+RSL+D I +N++ +E E + E+GQE FL AEAT+G+G+ EK AL +
Sbjct: 375 SLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKTALQK 434

Query: 432 LAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGICFGGL 491
           + +LS++G E+L+ +NKLDA+   GS +S V AIGG+PG++VPAGYD  G PYGI FGGL
Sbjct: 435 MKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGISFGGL 494

Query: 492 KGFEPRLIEIAYGFERLTKSRKPP 510
           +  EP+LIEIA+ FE+ T  RKPP
Sbjct: 495 RFSEPKLIEIAFAFEQATLIRKPP 508

BLAST of CSPI02G13250 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 5.5e-79
Identity = 204/513 (39.77%), Postives = 288/513 (56.14%), Query Frame = 0

Query: 32  LSLEEATLKDLQRAFYQNKLTSRQLVEFYLEQVRRFNPILKGIIEVNPDALDQASRADLE 91
           ++LE+AT+  LQ       LTS  +V  YL++  + NP + GI+++NPD L  AS  D E
Sbjct: 64  ITLEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELDDE 123

Query: 92  RKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRMAGAII 151
           R     R   PLHGIP +VKDN ATKDK++TTAGS+ALLGS+VPRDA VV +LR AGA++
Sbjct: 124 RANGIIR--GPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAGAVL 183

Query: 152 FGKASLSEWSYFRSNALPSGWSARGGQGKNPYTMG-EPCGSSSGSAISVAANMVTVSLGT 211
           FG A+LSEW+  RSN    G+SARGGQ + P+ +   P GSSSGSAISVA+NM+  +LGT
Sbjct: 184 FGHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFALGT 243

Query: 212 ETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISSRQDTVGPICRTVSDAAYVLEAIVGA 271
           ETDGSI+ P+  N VVG+KPTVGLTSR GV+P S  QDT GPI RTV DA YV +++ G 
Sbjct: 244 ETDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSMWGI 303

Query: 272 DRYDNSTIEASKYIPK-GGYGQFL-RAGGLKGKRIGI--VREFYDFGPDDTFYTQAYEKV 331
           D  D  T+  +   P+ G Y +FL     L+G R G+   R + +   D+        +V
Sbjct: 304 DENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGLPWKRLWQNAKADE---IDRLLEV 363

Query: 332 VKTLKKGGAILVDNLMIDNLEQIF-DG--------SSGEQIALLAEFKISLNAYLKELVA 391
           VK +++ GAI+ +N    NL+ I  DG        +  E   +  +F  ++ +YL E+  
Sbjct: 364 VKQIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEVKN 423

Query: 392 SPIRSLSDAIEFNKKNSELEN--------LEYGQEEFLKAEATNGI-GDAEKAALARLAK 451
           + I SL D +E+N K    E            GQ+ FL +    G+  +    A+  + +
Sbjct: 424 TEIHSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVEYVRR 483

Query: 452 LSKD-GFERLM-----------IKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGN 510
            S+D G +  +           I N L  V +  S+     A  G+P +++P G    G 
Sbjct: 484 TSQDEGIDYALNYTDPKTNDSFILNGL-LVPSGTSITYQQAAKAGYPMITLPIGVKTNGR 543

BLAST of CSPI02G13250 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 4.4e-68
Identity = 181/495 (36.57%), Postives = 263/495 (53.13%), Query Frame = 0

Query: 42  LQRAFYQNKLTSRQLVEFYLEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLS 101
           LQ  + Q  +    +V+ Y+ ++   N  ++ + E+NPDAL  A + D ERK    R   
Sbjct: 94  LQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNERKMGKLR--G 153

Query: 102 PLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWS 161
           PLHG+P+++K+NI T DK+++TAGS+A+ G+    DA V TKLR AG +I GK+  S+W+
Sbjct: 154 PLHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQWA 213

Query: 162 YFRSNALPSGWSARGGQGKNPYTMG-EPCGSSSGSAISVAANMVTVSLGTETDGSILCPS 221
            FRS    +GWSA GGQ    Y    +P GSSSGS ++    +   +LGTET GSI+ P+
Sbjct: 214 NFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSPA 273

Query: 222 TLNSVVGIKPTVGLTSRAGVVPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEA 281
             +++VG+KPTVGLTSR  VVPIS RQDTVGP+ R+V DAAY+L+ I G D  DN T  A
Sbjct: 274 DKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYT-SA 333

Query: 282 SKYIPKGGYGQFLRAGGLKGKRIGIVREFYD-FGPDDTFYTQAYEKVVKTLKKGGAILVD 341
             +     Y +      LKGKRIG+ R     FG   T   Q + + +  +KK GAI+V+
Sbjct: 334 IPFDTIPDYVKACDINALKGKRIGVPRNVIKIFGSPQTVVDQ-FNQALAVMKKAGAIIVE 393

Query: 342 NLMIDNLEQIFDGSSGEQIALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSELENL 401
           N    +  +       + I L A+   +L A+ K+L  +P  +++D     +        
Sbjct: 394 NTDFTSFAEFAQSPIPDDI-LYADSLTNLPAFFKQLKVNP-HNITDLESLRRFTQHHRLE 453

Query: 402 EYGQEEFLKAEAT--NGIGDAEKAALARLAKLSKDGFERLMI----KNKLDAVAAPGSLI 461
           EY   +  + +     GI + +        K  K G E  ++    ++KLDA   P  L 
Sbjct: 454 EYPSRDTARWDIALQKGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAVLPTDLS 513

Query: 462 SPVFAIGGFPGVSVPAGYDPQGN---------------PYGICFGGLKGFEPRLIEIAYG 514
             + A+ G P ++VP G  P G                P GI F G    E +LI +AY 
Sbjct: 514 PYIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWSEEKLIGLAYA 573

BLAST of CSPI02G13250 vs. ExPASy Swiss-Prot
Match: Q8RC40 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=gatA PE=3 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 9.1e-42
Identity = 148/506 (29.25%), Postives = 251/506 (49.60%), Query Frame = 0

Query: 32  LSLEEATLKDLQRAFYQNKLTSRQLVEFYLEQVRRFNPILKGIIEVNPD-ALDQASRADL 91
           + L   T+ +L+   ++ +++++++ + YLE+++   P +  ++ +  + AL++A  AD 
Sbjct: 1   MELYSLTVHELKELLHKREVSAQEVTKSYLERIKEVEPKIDALVTITEEFALERAKVADE 60

Query: 92  ERKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRMAGAI 151
             K    + L+   GIPV++KDNI+T + + TT  S  L   + P +A VV +L   G +
Sbjct: 61  MIKNGEAKDLT---GIPVIIKDNIST-EGIRTTCSSKMLENYIPPYNATVVERLLNEGVV 120

Query: 152 IFGKASLSEWSYFRSNALPSGWSARGGQGKNPYTMGE-PCGSSSGSAISVAANMVTVSLG 211
           I GK++L E++   S       ++     KNP+ +   P GSS GSA +VAA+    +LG
Sbjct: 121 ILGKSNLDEFAMGSSTE-----NSAFKTTKNPWDLSRVPGGSSGGSAAAVAADEAAFALG 180

Query: 212 TETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISSRQDTVGPICRTVSDAAYVLEAIVG 271
           ++T GSI  P++L  VVG+KPT GL SR G+V  +S  D +GP  + V+D A VL AI G
Sbjct: 181 SDTGGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNAIAG 240

Query: 272 ADRYDNSTIEASKYIPKGGYGQFLRAGGLKGKRIGIVREFYDFGPDDTFYTQAYEKVVKT 331
            D  D+++++    I K  Y  +L+   +KG RIG+ +EF+  G ++    +  EK +K 
Sbjct: 241 HDPMDSTSVK----IEKPDYTSYLKE-DIKGLRIGVAKEFFGAGIEEG-VKETVEKAIKV 300

Query: 332 LKKGGAILVDNLMIDNLEQIFDGSSGEQIALLAEFKISLNAYLKELVASPIRSLSDAIE- 391
            +  GA ++D + +  +E          I   AE   +L  Y         ++  D ++ 
Sbjct: 301 FEDLGAEIID-ISVPYVEYAL---PAYYIIASAEASSNLARYDGIRYGHIAKNYEDLVDM 360

Query: 392 FNKKNSELENLEYGQEEFLKAEA-TNGIGDAEKAALARLAKLSKDGFERLMIKNKLDAVA 451
           +    SE    E  +   L   A ++G  DA      ++  L K+ FER     K D + 
Sbjct: 361 YMTSRSEGFGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFERAF--EKCDVIV 420

Query: 452 APGS-----------------LISPVFA----IGGFPGVSVPAGYDPQGNPYGICFGGLK 511
            P S                  ++ ++     I G P +S+P G   +G P G+   G  
Sbjct: 421 GPTSPTVAFKIGERTNDPLAMYLADIYTVSVNIAGLPAISIPCGLS-EGLPVGLQIIGKH 480

BLAST of CSPI02G13250 vs. ExPASy Swiss-Prot
Match: B0K3S3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain X514) OX=399726 GN=gatA PE=3 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.6e-41
Identity = 151/506 (29.84%), Postives = 250/506 (49.41%), Query Frame = 0

Query: 32  LSLEEATLKDLQRAFYQNKLTSRQLVEFYLEQVRRFNPILKGIIEVNPD-ALDQASRADL 91
           + L   T+ +L+    + ++++ ++ + YLE+++   P +  ++ +  D AL +A  AD 
Sbjct: 1   MELYSLTIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQKAKEADE 60

Query: 92  ERKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRMAGAI 151
           + KK    +L+   GIPV++KDNI+T + + TT  S  L   + P +A VV KL   G I
Sbjct: 61  KIKKGEDTALT---GIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVI 120

Query: 152 IFGKASLSEWSYFRSNALPSGWSARGGQGKNPYTMGE-PCGSSSGSAISVAANMVTVSLG 211
           I GK++L E++   S       ++     KNP+ +   P GSS GSA ++AA+    +LG
Sbjct: 121 ILGKSNLDEFAMGSSTE-----NSAFKTTKNPWDLSRVPGGSSGGSAAAIAADEAAFALG 180

Query: 212 TETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISSRQDTVGPICRTVSDAAYVLEAIVG 271
           ++T GSI  P++L  VVG+KPT GL SR G+V  +S  D +GP  + V+D A VL  I+G
Sbjct: 181 SDTGGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIG 240

Query: 272 ADRYDNSTIEASKYIPKGGYGQFLRAGGLKGKRIGIVREFYDFGPDDTFYTQAYEKVVKT 331
            D  D+++++    I K  Y  +L+   +KG RIG+ +EF+  G ++    +  ++ +K 
Sbjct: 241 HDPKDSTSLK----IDKPDYTSYLKE-DIKGLRIGVAKEFFGEGIEEG-VKETVQESIKV 300

Query: 332 LKKGGAILVDNLMIDNLEQIFDGSSGEQIALLAEFKISLNAYLKELVASPIRSLSDAIE- 391
           L+  GA ++D + I  +E          I   AE   +L  Y             D I+ 
Sbjct: 301 LQDLGAEIID-ISIPYVEYAL---PAYYIIASAEASSNLARYDGIRYGHIAEKYEDLIDM 360

Query: 392 FNKKNSELENLEYGQEEFLKAEA-TNGIGDAEKAALARLAKLSKDGFERLMIKNKLDAVA 451
           +    SE    E  +   L   A ++G  DA      ++  L K+ FE+     K D + 
Sbjct: 361 YMVTRSEGFGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAF--EKCDVII 420

Query: 452 APGS-----------------LISPVFA----IGGFPGVSVPAGYDPQGNPYGICFGGLK 511
            P S                  ++ ++     I G PG+S+P G    G P G+   G  
Sbjct: 421 GPTSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGLS-DGLPVGLQIIGRH 480

BLAST of CSPI02G13250 vs. ExPASy TrEMBL
Match: A0A0A0LM97 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264020 PE=4 SV=1)

HSP 1 Score: 989.2 bits (2556), Expect = 6.5e-285
Identity = 512/514 (99.61%), Postives = 513/514 (99.81%), Query Frame = 0

Query: 1   MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY 60
           MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY
Sbjct: 1   MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY 60

Query: 61  LEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120
           LEQVRR NPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRLNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 121 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK 180
           NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK 180

Query: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300
           VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG
Sbjct: 241 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 301 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL 360
           KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL
Sbjct: 301 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL 360

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSELENLEYGQEEFLKAEATNGIGDAEK 420
           LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNS+LENLEYGQEEFLKAEATNGIGDAEK
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQEEFLKAEATNGIGDAEK 420

Query: 421 AALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGI 480
           AALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGI 480

Query: 481 CFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKRQ 515
           CFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKRQ
Sbjct: 481 CFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKRQ 514

BLAST of CSPI02G13250 vs. ExPASy TrEMBL
Match: A0A5D3BKT1 (Putative amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001390 PE=4 SV=1)

HSP 1 Score: 926.8 bits (2394), Expect = 4.0e-266
Identity = 480/511 (93.93%), Postives = 494/511 (96.67%), Query Frame = 0

Query: 1   MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY 60
           MAGQSFLL +SMLLGLMAILSS GSCSFHT+LS+EEATL DLQ AFYQNKLTSRQLVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 61  LEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120
           LEQVRRFNPILKGIIEVNPDALDQASRAD+ERKKSSPRSLSPLHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 121 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK 180
           NTTAGSFALLGS+VPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSN LPSGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPY +GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300
           +PISSRQD+VGPICRTVSDA YVL+AIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 301 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL 360
           KRIGIVREFYDFG D+TFY QAYEKV+KTLKKGGAILVDN  IDNL+ IFDGSSGEQIAL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSELENLEYGQEEFLKAEATNGIGDAEK 420
           LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNS+LENLEYGQE FLKAEATNGIGDAEK
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420

Query: 421 AALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGI 480
           AALARLAKLSKDGFERLMIKNKLDA+AAPGSLIS VFAIGGFPGVSVPAGYDPQG PYGI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYGI 480

Query: 481 CFGGLKGFEPRLIEIAYGFERLTKSRKPPSI 512
            FGGLKGFEPRLIEIAYGFE LTKSRKPPSI
Sbjct: 481 TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSI 511

BLAST of CSPI02G13250 vs. ExPASy TrEMBL
Match: E5GC09 (Amidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 926.8 bits (2394), Expect = 4.0e-266
Identity = 480/511 (93.93%), Postives = 494/511 (96.67%), Query Frame = 0

Query: 1   MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY 60
           MAGQSFLL +SMLLGLMAILSS GSCSFHT+LS+EEATL DLQ AFYQNKLTSRQLVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 61  LEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120
           LEQVRRFNPILKGIIEVNPDALDQASRAD+ERKKSSPRSLSPLHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 121 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK 180
           NTTAGSFALLGS+VPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSN LPSGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPY +GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300
           +PISSRQD+VGPICRTVSDA YVL+AIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 301 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL 360
           KRIGIVREFYDFG D+TFY QAYEKV+KTLKKGGAILVDN  IDNL+ IFDGSSGEQIAL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSELENLEYGQEEFLKAEATNGIGDAEK 420
           LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNS+LENLEYGQE FLKAEATNGIGDAEK
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420

Query: 421 AALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGI 480
           AALARLAKLSKDGFERLMIKNKLDA+AAPGSLIS VFAIGGFPGVSVPAGYDPQG PYGI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYGI 480

Query: 481 CFGGLKGFEPRLIEIAYGFERLTKSRKPPSI 512
            FGGLKGFEPRLIEIAYGFE LTKSRKPPSI
Sbjct: 481 TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSI 511

BLAST of CSPI02G13250 vs. ExPASy TrEMBL
Match: A0A1S3C5K8 (putative amidase C869.01 OS=Cucumis melo OX=3656 GN=LOC103497307 PE=4 SV=1)

HSP 1 Score: 926.8 bits (2394), Expect = 4.0e-266
Identity = 480/511 (93.93%), Postives = 494/511 (96.67%), Query Frame = 0

Query: 1   MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY 60
           MAGQSFLL +SMLLGLMAILSS GSCSFHT+LS+EEATL DLQ AFYQNKLTSRQLVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 61  LEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120
           LEQVRRFNPILKGIIEVNPDALDQASRAD+ERKKSSPRSLSPLHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 121 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK 180
           NTTAGSFALLGS+VPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSN LPSGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPY +GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300
           +PISSRQD+VGPICRTVSDA YVL+AIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 301 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL 360
           KRIGIVREFYDFG D+TFY QAYEKV+KTLKKGGAILVDN  IDNL+ IFDGSSGEQIAL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSELENLEYGQEEFLKAEATNGIGDAEK 420
           LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNS+LENLEYGQE FLKAEATNGIGDAEK
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420

Query: 421 AALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGI 480
           AALARLAKLSKDGFERLMIKNKLDA+AAPGSLIS VFAIGGFPGVSVPAGYDPQG PYGI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYGI 480

Query: 481 CFGGLKGFEPRLIEIAYGFERLTKSRKPPSI 512
            FGGLKGFEPRLIEIAYGFE LTKSRKPPSI
Sbjct: 481 TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSI 511

BLAST of CSPI02G13250 vs. ExPASy TrEMBL
Match: A0A5D3BN38 (Putative amidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001380 PE=4 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 6.2e-235
Identity = 438/516 (84.88%), Postives = 464/516 (89.92%), Query Frame = 0

Query: 1   MAGQSFLLDISMLLGLMAILS--SCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVE 60
           MA QSF + ISMLLGL+AILS    GSCSF T  S+EEATLKD Q AFYQNKLTSRQLVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKD 120
           FYLEQVRR NPILKGIIEVNPDAL+QAS+ADL+RK+SS RSLSPLHGIPVLVKDNIATKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQ 180
           KLNTTAGSFALLGS+VPRDAGVVTKLR AGAIIFGKASLSEWS FRS   P+GWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240
           GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGL 300
           GVVPIS RQDTVGPICRTV+DAAYVL+AI GADRYDNSTIEASKYIP+GGYGQFLRA GL
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 KGKRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQI 360
           KGKRIGIVR+ YDFG DD FY  A+EKV KTLK+GGAILVDNL I+  + I   SSGE  
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSELENL-EYGQEEFLKAEATNGIGD 420
           ALLAEFKISLNAYLK+LVASPIRSLSDAIEFNKKNS+LE L EYGQE FL+AEAT GIG 
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGG 420

Query: 421 AEKAALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNP 480
           AEKAALARLAKLSK+GFERLMIKNKLDA+AAPG LISP  AIGGFPGVSVPAGY+PQG P
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480

Query: 481 YGICFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKR 514
           +GI FGGLKGF+PRLIEIAYGFE LT  RK PS+ R
Sbjct: 481 FGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of CSPI02G13250 vs. NCBI nr
Match: XP_004147023.1 (probable amidase At4g34880 [Cucumis sativus] >KGN61907.1 hypothetical protein Csa_006678 [Cucumis sativus])

HSP 1 Score: 989.2 bits (2556), Expect = 1.4e-284
Identity = 512/514 (99.61%), Postives = 513/514 (99.81%), Query Frame = 0

Query: 1   MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY 60
           MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY
Sbjct: 1   MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY 60

Query: 61  LEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120
           LEQVRR NPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRLNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 121 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK 180
           NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK 180

Query: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300
           VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG
Sbjct: 241 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 301 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL 360
           KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL
Sbjct: 301 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL 360

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSELENLEYGQEEFLKAEATNGIGDAEK 420
           LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNS+LENLEYGQEEFLKAEATNGIGDAEK
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQEEFLKAEATNGIGDAEK 420

Query: 421 AALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGI 480
           AALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGI 480

Query: 481 CFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKRQ 515
           CFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKRQ
Sbjct: 481 CFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKRQ 514

BLAST of CSPI02G13250 vs. NCBI nr
Match: XP_008457661.1 (PREDICTED: putative amidase C869.01 [Cucumis melo] >ADN34008.1 amidase [Cucumis melo subsp. melo] >KAA0045711.1 putative amidase [Cucumis melo var. makuwa] >TYJ99569.1 putative amidase [Cucumis melo var. makuwa])

HSP 1 Score: 926.8 bits (2394), Expect = 8.2e-266
Identity = 480/511 (93.93%), Postives = 494/511 (96.67%), Query Frame = 0

Query: 1   MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY 60
           MAGQSFLL +SMLLGLMAILSS GSCSFHT+LS+EEATL DLQ AFYQNKLTSRQLVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 61  LEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120
           LEQVRRFNPILKGIIEVNPDALDQASRAD+ERKKSSPRSLSPLHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 121 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK 180
           NTTAGSFALLGS+VPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSN LPSGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPY +GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300
           +PISSRQD+VGPICRTVSDA YVL+AIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 301 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL 360
           KRIGIVREFYDFG D+TFY QAYEKV+KTLKKGGAILVDN  IDNL+ IFDGSSGEQIAL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSELENLEYGQEEFLKAEATNGIGDAEK 420
           LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNS+LENLEYGQE FLKAEATNGIGDAEK
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420

Query: 421 AALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGI 480
           AALARLAKLSKDGFERLMIKNKLDA+AAPGSLIS VFAIGGFPGVSVPAGYDPQG PYGI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYGI 480

Query: 481 CFGGLKGFEPRLIEIAYGFERLTKSRKPPSI 512
            FGGLKGFEPRLIEIAYGFE LTKSRKPPSI
Sbjct: 481 TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSI 511

BLAST of CSPI02G13250 vs. NCBI nr
Match: XP_038900912.1 (LOW QUALITY PROTEIN: probable amidase At4g34880 [Benincasa hispida])

HSP 1 Score: 825.1 bits (2130), Expect = 3.4e-235
Identity = 430/515 (83.50%), Postives = 466/515 (90.49%), Query Frame = 0

Query: 1   MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY 60
           MA QSF L ISMLL L+AILSS GSCSF T LS+EEAT+KDLQ AFYQNKL SRQLV+FY
Sbjct: 6   MAAQSFWLYISMLLSLLAILSSYGSCSFDTHLSIEEATVKDLQLAFYQNKLISRQLVKFY 65

Query: 61  LEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120
           L  VRRFNPILKGIIEVNPDALDQAS+ADLERK++SP SLSPLHGIPVLVKDNIAT+DKL
Sbjct: 66  LNXVRRFNPILKGIIEVNPDALDQASQADLERKRNSPSSLSPLHGIPVLVKDNIATRDKL 125

Query: 121 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK 180
           NTTAGSFALLGS+VPRDAGVVTKLR AGAIIFGKASLS+WSYFRS  +PSGWSARGGQGK
Sbjct: 126 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSKWSYFRSYXIPSGWSARGGQGK 185

Query: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPYTMGEPCGSS GSAIS+AANMV VSLGTETDGSILCPS LNSVVGIKPTVGLTSRAGV
Sbjct: 186 NPYTMGEPCGSSGGSAISIAANMVAVSLGTETDGSILCPSILNSVVGIKPTVGLTSRAGV 245

Query: 241 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300
           +PIS RQDTVGPICRTVSD AYVL AIVGAD+ DNSTIEASKYIP+GGY QFLRA GLKG
Sbjct: 246 IPISLRQDTVGPICRTVSDDAYVLGAIVGADKNDNSTIEASKYIPRGGYAQFLRADGLKG 305

Query: 301 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL 360
           KRIGIVR+F+DFG DD FY +AYEKV KTL++GGA+ VDNL ID+L+ I   SSGE+ AL
Sbjct: 306 KRIGIVRKFFDFGHDDAFYPRAYEKVFKTLRQGGAVFVDNLTIDSLQVIMGSSSGEETAL 365

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSELENL-EYGQEEFLKAEATNGIGDAE 420
           LAEFKISLNAYLK+LVASPIRSLSDAIEFN+KNS+LE L EYGQ  F++AEAT GIGDAE
Sbjct: 366 LAEFKISLNAYLKQLVASPIRSLSDAIEFNEKNSKLEKLREYGQNLFIEAEATKGIGDAE 425

Query: 421 KAALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYG 480
           KAALARLAKLSKDGFER+M+KNKLDA+AAPG  IS V AIGGFPG+SVPAGY+ QG P+G
Sbjct: 426 KAALARLAKLSKDGFERVMLKNKLDAIAAPGWEISSVLAIGGFPGISVPAGYNSQGVPFG 485

Query: 481 ICFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKRQ 515
           I FGGLKGFEPRLIEIAYGFE LTKSRK PSIKR+
Sbjct: 486 ISFGGLKGFEPRLIEIAYGFEHLTKSRKSPSIKRR 520

BLAST of CSPI02G13250 vs. NCBI nr
Match: XP_008457659.1 (PREDICTED: putative amidase C869.01 isoform X1 [Cucumis melo] >KAA0045712.1 putative amidase isoform X1 [Cucumis melo var. makuwa] >TYJ99568.1 putative amidase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 823.2 bits (2125), Expect = 1.3e-234
Identity = 438/516 (84.88%), Postives = 464/516 (89.92%), Query Frame = 0

Query: 1   MAGQSFLLDISMLLGLMAILS--SCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVE 60
           MA QSF + ISMLLGL+AILS    GSCSF T  S+EEATLKD Q AFYQNKLTSRQLVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKD 120
           FYLEQVRR NPILKGIIEVNPDAL+QAS+ADL+RK+SS RSLSPLHGIPVLVKDNIATKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQ 180
           KLNTTAGSFALLGS+VPRDAGVVTKLR AGAIIFGKASLSEWS FRS   P+GWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240
           GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGL 300
           GVVPIS RQDTVGPICRTV+DAAYVL+AI GADRYDNSTIEASKYIP+GGYGQFLRA GL
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 KGKRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQI 360
           KGKRIGIVR+ YDFG DD FY  A+EKV KTLK+GGAILVDNL I+  + I   SSGE  
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSELENL-EYGQEEFLKAEATNGIGD 420
           ALLAEFKISLNAYLK+LVASPIRSLSDAIEFNKKNS+LE L EYGQE FL+AEAT GIG 
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGG 420

Query: 421 AEKAALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNP 480
           AEKAALARLAKLSK+GFERLMIKNKLDA+AAPG LISP  AIGGFPGVSVPAGY+PQG P
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480

Query: 481 YGICFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKR 514
           +GI FGGLKGF+PRLIEIAYGFE LT  RK PS+ R
Sbjct: 481 FGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of CSPI02G13250 vs. NCBI nr
Match: XP_004147024.3 (probable amidase At4g34880 [Cucumis sativus])

HSP 1 Score: 822.8 bits (2124), Expect = 1.7e-234
Identity = 433/514 (84.24%), Postives = 462/514 (89.88%), Query Frame = 0

Query: 1   MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY 60
           MA QSF + ISMLLGL+AILSS GSCSF T  S+EEATLKDLQ AFYQNKLTS QLVEFY
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 61  LEQVRRFNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120
           LEQVRRFNPIL GIIEVNPDAL+QAS+ADLERK+SSPRSLSPLHGIPVLVKDNIATKD+L
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDQL 120

Query: 121 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK 180
           NTTAGSFALLGS+VPRDAGVVTKLR AGAIIFGKASLSEWS FRS   P+GWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQGK 180

Query: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300
           VPIS RQDTVGPICRTV+DAAYVL+AI G DRYDNSTIEASKY+PKGGYGQFL+  GLKG
Sbjct: 241 VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGLKG 300

Query: 301 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL 360
           KRIGIVR+ YDFG DD FY  A+EKV KTLK+GGAILVDNL I++   I   SSGE  A+
Sbjct: 301 KRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGSSSGEWTAV 360

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSELENL-EYGQEEFLKAEATNGIGDAE 420
           LAEFKIS+N YLK+LVASPIRSLSDAIEFN+KNS+LE L EYGQE FL+AEAT GIG AE
Sbjct: 361 LAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATKGIGGAE 420

Query: 421 KAALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYG 480
           KAALARLAKLSK+GFERLMIKNKLDA+AAPG LIS   AIGGFPGVSVPAGY+PQG P+G
Sbjct: 421 KAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNPQGLPFG 480

Query: 481 ICFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKR 514
           I FGGLKGFEPRLIEIAYGFE LT  RK PS+ R
Sbjct: 481 IGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSLGR 514

BLAST of CSPI02G13250 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 479.2 bits (1232), Expect = 4.2e-135
Identity = 268/503 (53.28%), Postives = 350/503 (69.58%), Query Frame = 0

Query: 12  MLLGLMAILSSCGSCS---FHTELSLEEATLKDLQRAFYQNKLTSRQLVEFYLEQVRRFN 71
           +L+   A++ S GS S     +  S++EAT++D++ AF + +LTS+QLVE YLE + + N
Sbjct: 15  LLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLEAISKLN 74

Query: 72  PILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFA 131
           PIL  +IE NPDAL QA  AD ER   +   L  LHG+PVL+KD+I+TKDKLNTTAGSFA
Sbjct: 75  PILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNTTAGSFA 134

Query: 132 LLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGKNPYTMGEP 191
           LLGSVV RDAGVV +LR +GA+I GKASLSEW++FRS ++P GWSA              
Sbjct: 135 LLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA-------------- 194

Query: 192 CGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISSRQD 251
                                         PS  NSVVGIKP+VGLTSRAGVVPIS RQD
Sbjct: 195 ------------------------------PSQ-NSVVGIKPSVGLTSRAGVVPISLRQD 254

Query: 252 TVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKGKRIGIVRE 311
           ++GPICRTVSDA ++L+AIVG D  D +T  AS++IP+GGY QFL   GLKGKR+GIV +
Sbjct: 255 SIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGKRLGIVMK 314

Query: 312 FYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGS-SGEQIALLAEFKIS 371
                     ++   +  +KTL++ GAI+++NL I N+E I  G+ SGE+IALLAEFK+S
Sbjct: 315 ----------HSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLAEFKMS 374

Query: 372 LNAYLKELVASPIRSLSDAIEFNKKNSELENL-EYGQEEFLKAEATNGIGDAEKAALARL 431
           LNAYLKELV SP+RSL+D I +N++ +E E + E+GQE FL AEAT+G+G+ EK AL ++
Sbjct: 375 LNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKTALQKM 434

Query: 432 AKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGICFGGLK 491
            +LS++G E+L+ +NKLDA+   GS +S V AIGG+PG++VPAGYD  G PYGI FGGL+
Sbjct: 435 KELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGISFGGLR 462

Query: 492 GFEPRLIEIAYGFERLTKSRKPP 510
             EP+LIEIA+ FE+ T  RKPP
Sbjct: 495 FSEPKLIEIAFAFEQATLIRKPP 462

BLAST of CSPI02G13250 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 130.2 bits (326), Expect = 4.8e-30
Identity = 140/520 (26.92%), Postives = 237/520 (45.58%), Query Frame = 0

Query: 19  ILSSCGSCSFHTE-LSLEEATLKDLQRAFYQNKLTSRQLVEFYLEQVRRFNPILKGIIEV 78
           I+S+  S +  T  +S  ++ +   +R+    + T+ ++ + YL ++R   P LK  + V
Sbjct: 25  IVSAASSKTIDTSVISPPQSQILTTRRSLLSGETTAVEIAKSYLSRIRLTEPQLKCFLHV 84

Query: 79  NPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRD 138
           + + L  A   +++++ +    L PL G+ + VKDNI T+  + +TA S  L     P D
Sbjct: 85  SENVLKDAQ--EIDQRIAKGEELGPLAGVLIGVKDNICTQG-MPSTAASRILEHYRPPFD 144

Query: 139 AGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGKNPYTMGE-PCGSSSGSA 198
           A  V K++  G I+ GK ++ E+    S    S +        NP+ +   P GSS GSA
Sbjct: 145 ATAVKKIKELGGIVVGKTNMDEFG-MGSTTEASAFQVTA----NPWDLSRVPGGSSGGSA 204

Query: 199 ISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISSRQDTVGPICRT 258
            +VAA    VSLG++T GS+  P++   VVG+KPT G  SR G++  +S  D +G    T
Sbjct: 205 AAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCFGST 264

Query: 259 VSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFL-----RAGGLKGKRIGIVREFYD 318
           V+DA  +L AI G DR+D++   +SK        QFL      +  L G ++GI+RE  +
Sbjct: 265 VADAGMLLHAISGYDRFDST---SSKQDVPEFQSQFLSVDHFESKPLNGVKVGIIRETLE 324

Query: 319 FGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIALLAEFKISLNAY 378
            G  D+    A ++    L+  G IL +     +L     G     +   +E   +L+ Y
Sbjct: 325 DGV-DSGVRSATQEAASHLEALGCILTE----VSLPSFSLGLPAYYVIASSESSSNLSRY 384

Query: 379 LKELVASPIRSLSDAIEFNKKNSELENLEYGQEEFLKA-----EATNGIGDAEKAALARL 438
                 + +     A E NK         +G E  ++        + G  DA      ++
Sbjct: 385 DGVRYGNQVM----AEELNKLYECSRGEGFGGEVKMRILMGTYALSAGYYDAYYKRAQQV 444

Query: 439 AKLSKDGFERLMIKNK-LDAVAAP------------------GSLISPVFAIGGFPGVSV 498
             L +  F+  + +N  L + AAP                  G +++    + G P + +
Sbjct: 445 RTLIRKDFKAALEQNDILISPAAPSAAYKIGEKKDDPLAMYAGDIMTVNVNLAGLPAMVL 504

Query: 499 PAGY---DPQGNPYGICFGGLKGFEPRLIEIAYGFERLTK 505
           P G     P G P G+   G    E +L+++ + FE+  K
Sbjct: 505 PCGLVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIFEQTLK 524

BLAST of CSPI02G13250 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 120.9 bits (302), Expect = 2.9e-27
Identity = 82/232 (35.34%), Postives = 126/232 (54.31%), Query Frame = 0

Query: 50  KLTSRQLVEFYLEQVRRFNPILKGIIEVNPD-ALDQASRADLERKKSSPRSLSPLHGIPV 109
           ++TS++LV  YL+Q++R+N +L+ ++    + A  QA  AD     S    L PLHGIP 
Sbjct: 216 QITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEAD--DLLSQGTYLGPLHGIPY 275

Query: 110 LVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNAL 169
            +KD +A      TT GS +     +  +A V  +L+ +GA++  K      +Y      
Sbjct: 276 GLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSMAY------ 335

Query: 170 PSGWSARGGQGKNPYTMGE-PCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVG 229
              W   GG+ +NP+ + E   GSS+G A S +A MV  ++G+ET GS+  P+    +  
Sbjct: 336 DDIWF--GGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAGSMTYPAARCGITA 395

Query: 230 IKPTVGLTSRAGVVPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIE 280
           ++PT G   R GV+ IS   D +GP CRT +D A +L+AI G D  D S+ E
Sbjct: 396 LRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSRE 436

BLAST of CSPI02G13250 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 110.5 bits (275), Expect = 4.0e-24
Identity = 80/232 (34.48%), Postives = 122/232 (52.59%), Query Frame = 0

Query: 50  KLTSRQLVEFYLEQVRRFNPILKGIIEVNPD-ALDQASRADLERKKSSPRSLSPLHGIPV 109
           ++TS++LV  YL+Q++R+N +L+ ++    + A  QA  AD     S    L PLHGIP 
Sbjct: 216 QITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEAD--DLLSQGTYLGPLHGIPY 275

Query: 110 LVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNAL 169
            +KD +A      TT GS +     +  +A V  +L+ +GA++  K      +Y      
Sbjct: 276 GLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSMAY------ 335

Query: 170 PSGWSARGGQGKNPYTMGE-PCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVG 229
              W   GG+ +NP+ + E   GSS+G A S +A       G+ET GS+  P+    +  
Sbjct: 336 DDIWF--GGRTRNPWNIEEFSTGSSAGPAASTSA-------GSETAGSMTYPAARCGITA 395

Query: 230 IKPTVGLTSRAGVVPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIE 280
           ++PT G   R GV+ IS   D +GP CRT +D A +L+AI G D  D S+ E
Sbjct: 396 LRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSRE 429

BLAST of CSPI02G13250 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 96.7 bits (239), Expect = 5.9e-20
Identity = 114/493 (23.12%), Postives = 202/493 (40.97%), Query Frame = 0

Query: 39  LKDLQRAFYQNKLTSRQLVEFYLEQVRRF---NPILKGIIEVNPDALDQASRADLERKKS 98
           ++D   A+     T  Q+ +  +  +  F    P    +I  + + + + + A   R + 
Sbjct: 131 IRDYAYAYRSKLTTPLQVAKRIISIIEEFGYDKPPTPFLIRFDANEVIKQAEASTRRFEQ 190

Query: 99  SPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKA 158
               +S L GI V +KD+I           ++      V +D+ VV+KLR  GAI+ GKA
Sbjct: 191 G-NPISVLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKA 250

Query: 159 SLSEWSYFRSNALPSGWSARGGQGKNPYTMGE-PCGSSSGSAISVAANMVTVSLGTETDG 218
           ++ E          +G ++  G  +NP+       GSSSGSA  VAA + + +LGT+  G
Sbjct: 251 NMHELGMG-----TTGNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAAGLCSAALGTDGGG 310

Query: 219 SILCPSTLNSVVGIKPTVGLTSRAGVVPISSRQDTVGPICRTVSDAAYVLEAIVGADRYD 278
           S+  PS L  + G+K T G T   G +      + +GP+  ++ DA  V  AI+G+   D
Sbjct: 311 SVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAFLVYAAILGSSSAD 370

Query: 279 NSTIEAS-----KYIPKGGYGQFLRAGGLKGKRIG-IVREFYDFGPDDTFYTQAYEKVVK 338
              ++ S     K +   G      +  +   R+G   + F D    D   +   E ++K
Sbjct: 371 RYNLKPSPPCFPKLLSHNG------SNAIGSLRLGKYTKWFNDVSSSD--ISDKCEDILK 430

Query: 339 TLKKGGAILVDNLMIDNLEQIFDGSSGEQIALLAEFKISLNAYLKELVASPIRSLSDAIE 398
            L       V  +++  LE++    +   I++ +    SL  Y +          +  + 
Sbjct: 431 LLSNNHGCKVVEIVVPELEEM---RAAHVISIGSPTLSSLTPYCE-------AGKNSKLS 490

Query: 399 FNKKNSELENLEYGQEEFLKAEATN--------GIGDAEKAALARLAKLSKDGFERLMIK 458
           ++ + S      +   +++ A+            I       +     ++        +K
Sbjct: 491 YDTRTSFAIFRSFSASDYIAAQCLRRRLMEYHLNIFKDVDVIVTPTTGMTAPVIPPDALK 550

Query: 459 NKLDAVAAPGSLISPVFAIG--GFPGVSVPAGYDPQGNPYGICFGGLKGFEPRLIEIAYG 512
           N    +     L+  V A    GFP +SVP GYD +G P G+   G    E  ++ +A  
Sbjct: 551 NGETNIQVTTDLMRFVLAANLLGFPAISVPVGYDKEGLPIGLQIMGRPWAEATVLGLAAA 599