CSPI02G13120 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G13120
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionTransmembrane protein
LocationChr2: 13221401 .. 13221921 (-)
RNA-Seq ExpressionCSPI02G13120
SyntenyCSPI02G13120
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTATTTCATGACACTATAGAATTTTCAAGTGAGAAATGGATTGTTTTTATCCAAAAAGAAGAGGTCCAGAGTGGAAACGAGGATTGACAGGCACAGGCCAAACCCTAAGCTCTGTTTCTCCTCCACCAATTCAATTGCTCATCATTTTTGGCATTGTCATTGTGTTGCTATGGTTTTCTCAGTTCAATGCTGGCCATGAAGCTCAATTGAATAGAAGTTTTCAGCTGTTTCTTTTCTTGTTGCCCATCTTCGTTATTTTCTTCATGTATTCATATTCCTCCAGTCGACTTATCAACTTCGGATTTCGCCGATCACCAACCGGGTTGGCTCAACGAGGTGCCTCGCCTTGGACTATTGCCATTTTGGTCCTTCTCATTCTGGTTTTGCTTTATTATCAATCCTCATTCCATTGAAAGCAGACTAATCTTACAAAGCTTGTGATATGCATGGTATGAGTATTAATTTTCAACCTCATGGTGGAGTTTTTTAGCGTATGAATAATGTCATAATAATTTTAAA

mRNA sequence

CTCTATTTCATGACACTATAGAATTTTCAAGTGAGAAATGGATTGTTTTTATCCAAAAAGAAGAGGTCCAGAGTGGAAACGAGGATTGACAGGCACAGGCCAAACCCTAAGCTCTGTTTCTCCTCCACCAATTCAATTGCTCATCATTTTTGGCATTGTCATTGTGTTGCTATGGTTTTCTCAGTTCAATGCTGGCCATGAAGCTCAATTGAATAGAAGTTTTCAGCTGTTTCTTTTCTTGTTGCCCATCTTCGTTATTTTCTTCATGTATTCATATTCCTCCAGTCGACTTATCAACTTCGGATTTCGCCGATCACCAACCGGGTTGGCTCAACGAGGTGCCTCGCCTTGGACTATTGCCATTTTGGTCCTTCTCATTCTGGTTTTGCTTTATTATCAATCCTCATTCCATTGAAAGCAGACTAATCTTACAAAGCTTGTGATATGCATGGTATGAGTATTAATTTTCAACCTCATGGTGGAGTTTTTTAGCGTATGAATAATGTCATAATAATTTTAAA

Coding sequence (CDS)

ATGGATTGTTTTTATCCAAAAAGAAGAGGTCCAGAGTGGAAACGAGGATTGACAGGCACAGGCCAAACCCTAAGCTCTGTTTCTCCTCCACCAATTCAATTGCTCATCATTTTTGGCATTGTCATTGTGTTGCTATGGTTTTCTCAGTTCAATGCTGGCCATGAAGCTCAATTGAATAGAAGTTTTCAGCTGTTTCTTTTCTTGTTGCCCATCTTCGTTATTTTCTTCATGTATTCATATTCCTCCAGTCGACTTATCAACTTCGGATTTCGCCGATCACCAACCGGGTTGGCTCAACGAGGTGCCTCGCCTTGGACTATTGCCATTTTGGTCCTTCTCATTCTGGTTTTGCTTTATTATCAATCCTCATTCCATTGA

Protein sequence

MDCFYPKRRGPEWKRGLTGTGQTLSSVSPPPIQLLIIFGIVIVLLWFSQFNAGHEAQLNRSFQLFLFLLPIFVIFFMYSYSSSRLINFGFRRSPTGLAQRGASPWTIAILVLLILVLLYYQSSFH*
Homology
BLAST of CSPI02G13120 vs. ExPASy TrEMBL
Match: A0A0A0LLH8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G263890 PE=4 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 3.7e-64
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0

Query: 1   MDCFYPKRRGPEWKRGLTGTGQTLSSVSPPPIQLLIIFGIVIVLLWFSQFNAGHEAQLNR 60
           MDCFYPKRRGPEWKRGLTGTGQTLSSVSPPPIQLLIIFGIVIVLLWFSQFNAGHEAQLNR
Sbjct: 1   MDCFYPKRRGPEWKRGLTGTGQTLSSVSPPPIQLLIIFGIVIVLLWFSQFNAGHEAQLNR 60

Query: 61  SFQLFLFLLPIFVIFFMYSYSSSRLINFGFRRSPTGLAQRGASPWTIAILVLLILVLLYY 120
           SFQLFLFLLPIFVIFFMYSYSSSRLINFGFRRSPTGLAQRGASPWTIAILVLLILVLLYY
Sbjct: 61  SFQLFLFLLPIFVIFFMYSYSSSRLINFGFRRSPTGLAQRGASPWTIAILVLLILVLLYY 120

Query: 121 QSSFH 126
           QSSFH
Sbjct: 121 QSSFH 125

BLAST of CSPI02G13120 vs. ExPASy TrEMBL
Match: A0A2P5C233 (Transmembrane protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_190300 PE=4 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.9e-28
Identity = 75/130 (57.69%), Postives = 92/130 (70.77%), Query Frame = 0

Query: 1   MDCFYPKRRGPEWKRGLTGTGQTLSSVSPPPIQLLIIFGIVIVLLWFSQFNAGHEAQLNR 60
           M  FYPKRRGP+WK+G   T QT++SVS P   LL IF IV+VLLW SQ+  GH+AQL +
Sbjct: 1   MGWFYPKRRGPQWKQG--WTSQTVASVSAPSFHLLAIFSIVMVLLWLSQY-TGHKAQLQQ 60

Query: 61  ---SFQLFLFLLPIFVIFFMYSYSSSRLINFGFR--RSPTGLAQRGASPWTIAILVLLIL 120
              +FQLF+FLLP+ +I FM SYSS  L NF FR  R  T +   G+SPW +A+ V L+L
Sbjct: 61  TAVNFQLFVFLLPVLLIVFMASYSSCGLFNFRFRRPRPDTPMQPAGSSPWGVAVFVALLL 120

Query: 121 VLLYYQSSFH 126
           VLL YQSSFH
Sbjct: 121 VLLSYQSSFH 127

BLAST of CSPI02G13120 vs. ExPASy TrEMBL
Match: A0A2P5ED06 (Transmembrane protein OS=Trema orientale OX=63057 GN=TorRG33x02_208280 PE=4 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 3.3e-28
Identity = 74/130 (56.92%), Postives = 93/130 (71.54%), Query Frame = 0

Query: 1   MDCFYPKRRGPEWKRGLTGTGQTLSSVSPPPIQLLIIFGIVIVLLWFSQFNAGHEAQLNR 60
           M+ FYPKRRGP+WK+G   T QT++S+S P   LL IF IV+VLLW SQ+  G++AQL +
Sbjct: 1   MEWFYPKRRGPQWKQG--WTSQTVASISAPSFHLLAIFSIVMVLLWLSQY-TGYKAQLQQ 60

Query: 61  ---SFQLFLFLLPIFVIFFMYSYSSSRLINFGFR--RSPTGLAQRGASPWTIAILVLLIL 120
              +FQLF+FLLP+ +I FM SYSSS L NF FR  R  T +   G+SPW +A  V L+L
Sbjct: 61  TAVNFQLFMFLLPVLLIVFMASYSSSGLFNFRFRRPRPDTPMQLAGSSPWGVAFFVALLL 120

Query: 121 VLLYYQSSFH 126
           VLL YQSSFH
Sbjct: 121 VLLSYQSSFH 127

BLAST of CSPI02G13120 vs. ExPASy TrEMBL
Match: W9QKI2 (Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_024950 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 5.6e-28
Identity = 74/132 (56.06%), Postives = 93/132 (70.45%), Query Frame = 0

Query: 1   MDCFYPKRRGPEWKRGLTGTGQTLSSVSPPPIQLLIIFGIVIVLLWFSQFNAGHEAQLNR 60
           M+ FYPKRRGPEWK+G   T QT++S+SPPP  LL IF IV+VLLW SQ+  G++AQL +
Sbjct: 1   MEWFYPKRRGPEWKQG--WTSQTMASISPPPFHLLTIFAIVMVLLWLSQY-TGYKAQLQQ 60

Query: 61  ---SFQLFLFLLPIFVIFFMYSYSSSRLINFGFRRSPTGLAQR----GASPWTIAILVLL 120
              +FQ FLFL PI +I FM SYSSS   NF  RR     + +    G+SPW +AI V+L
Sbjct: 61  TTANFQFFLFLFPILLILFMASYSSSWWPNFQLRRVRQEASMQPVAAGSSPWGVAIFVVL 120

Query: 121 ILVLLYYQSSFH 126
           +L+LL YQSSFH
Sbjct: 121 LLILLSYQSSFH 129

BLAST of CSPI02G13120 vs. ExPASy TrEMBL
Match: B9H148 (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_004G167800 PE=4 SV=2)

HSP 1 Score: 133.3 bits (334), Expect = 7.2e-28
Identity = 75/130 (57.69%), Postives = 92/130 (70.77%), Query Frame = 0

Query: 1   MDCFYPKRRGPEWKRGLTGTGQTLSSVSPPPIQLLIIFGIVIVLLWFSQFNAGHEAQLNR 60
           M+ FYPKRRGPEWK+G   TGQT++S+S PP  LL IFGIVI LLW S +  G+EAQL  
Sbjct: 1   MEWFYPKRRGPEWKQG--WTGQTVASMSTPPFPLLAIFGIVISLLWLSHY-TGYEAQLRH 60

Query: 61  S---FQLFLFLLPIFVIFFMYSYSSSRLINFGFRRSPTGLAQRGAS--PWTIAILVLLIL 120
           S    Q+FLFLLPI +I FM SYS++ L+ +  R SP   A R  S  PW IA+ V+L+L
Sbjct: 61  SAINLQIFLFLLPILLILFMASYSTNWLLYYRLRDSPPDSAPRSTSSFPWGIAVFVVLLL 120

Query: 121 VLLYYQSSFH 126
           +LL YQSSFH
Sbjct: 121 MLLSYQSSFH 127

BLAST of CSPI02G13120 vs. NCBI nr
Match: KGN61894.1 (hypothetical protein Csa_005908 [Cucumis sativus])

HSP 1 Score: 253.8 bits (647), Expect = 7.6e-64
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0

Query: 1   MDCFYPKRRGPEWKRGLTGTGQTLSSVSPPPIQLLIIFGIVIVLLWFSQFNAGHEAQLNR 60
           MDCFYPKRRGPEWKRGLTGTGQTLSSVSPPPIQLLIIFGIVIVLLWFSQFNAGHEAQLNR
Sbjct: 1   MDCFYPKRRGPEWKRGLTGTGQTLSSVSPPPIQLLIIFGIVIVLLWFSQFNAGHEAQLNR 60

Query: 61  SFQLFLFLLPIFVIFFMYSYSSSRLINFGFRRSPTGLAQRGASPWTIAILVLLILVLLYY 120
           SFQLFLFLLPIFVIFFMYSYSSSRLINFGFRRSPTGLAQRGASPWTIAILVLLILVLLYY
Sbjct: 61  SFQLFLFLLPIFVIFFMYSYSSSRLINFGFRRSPTGLAQRGASPWTIAILVLLILVLLYY 120

Query: 121 QSSFH 126
           QSSFH
Sbjct: 121 QSSFH 125

BLAST of CSPI02G13120 vs. NCBI nr
Match: KAG7010871.1 (hypothetical protein SDJN02_27669, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 143.3 bits (360), Expect = 1.4e-30
Identity = 84/129 (65.12%), Postives = 93/129 (72.09%), Query Frame = 0

Query: 1   MDCFYPKRRGPEWKRGLTGTGQTL-SSVSPPPIQLLIIFGIVIVLLWFSQFNAGHEAQLN 60
           M+   PKRRGPEWK+    TGQTL  SVS PP QLL IFGIVIVLLWFSQ   G++ Q++
Sbjct: 1   MEWLEPKRRGPEWKQ--EWTGQTLGGSVSSPPPQLLTIFGIVIVLLWFSQSTTGYDTQMH 60

Query: 61  RS---FQLFLFLLPIFVIFFMYSYSSSRLINFGFRRSPTGLAQRGASPWTIAILVLLILV 120
           RS   FQL LFLLPI VIFFM SYS   LIN    RS TG  +RGASP TI ILV LILV
Sbjct: 61  RSASNFQLLLFLLPILVIFFMSSYSMCGLINLRSWRSATGSPRRGASPLTITILVSLILV 120

Query: 121 LLYYQSSFH 126
           LLY++ SFH
Sbjct: 121 LLYFRPSFH 127

BLAST of CSPI02G13120 vs. NCBI nr
Match: KAF3434655.1 (hypothetical protein FNV43_RR21740 [Rhamnella rubrinervis])

HSP 1 Score: 142.1 bits (357), Expect = 3.2e-30
Identity = 80/129 (62.02%), Postives = 97/129 (75.19%), Query Frame = 0

Query: 1   MDCFYPKRRGPEWKRGLTGTGQTLSSVSPPPIQLLIIFGIVIVLLWFSQFNAGHEAQLNR 60
           M+ FYPKRRGPEWK+G   TGQTL+S+S PP+ LL IFGIVIVLLWFSQ+  G++AQL +
Sbjct: 1   MEWFYPKRRGPEWKQG--WTGQTLTSISAPPLHLLTIFGIVIVLLWFSQY-TGYKAQLQQ 60

Query: 61  ---SFQLFLFLLPIFVIFFMYSYSSS-RLINFGFRRSPTGLAQRGASPWTIAILVLLILV 120
              +FQLFL +LPI +IFFM  YSSS    NF  RR P    + G SPW +AIL++L+LV
Sbjct: 61  TTVNFQLFLMVLPILLIFFMALYSSSGGWFNFQLRR-PEAFHRAGGSPWGVAILLVLLLV 120

Query: 121 LLYYQSSFH 126
           LL YQSSFH
Sbjct: 121 LLSYQSSFH 125

BLAST of CSPI02G13120 vs. NCBI nr
Match: PON55102.1 (Transmembrane protein [Parasponia andersonii])

HSP 1 Score: 135.2 bits (339), Expect = 3.9e-28
Identity = 75/130 (57.69%), Postives = 92/130 (70.77%), Query Frame = 0

Query: 1   MDCFYPKRRGPEWKRGLTGTGQTLSSVSPPPIQLLIIFGIVIVLLWFSQFNAGHEAQLNR 60
           M  FYPKRRGP+WK+G   T QT++SVS P   LL IF IV+VLLW SQ+  GH+AQL +
Sbjct: 1   MGWFYPKRRGPQWKQG--WTSQTVASVSAPSFHLLAIFSIVMVLLWLSQY-TGHKAQLQQ 60

Query: 61  ---SFQLFLFLLPIFVIFFMYSYSSSRLINFGFR--RSPTGLAQRGASPWTIAILVLLIL 120
              +FQLF+FLLP+ +I FM SYSS  L NF FR  R  T +   G+SPW +A+ V L+L
Sbjct: 61  TAVNFQLFVFLLPVLLIVFMASYSSCGLFNFRFRRPRPDTPMQPAGSSPWGVAVFVALLL 120

Query: 121 VLLYYQSSFH 126
           VLL YQSSFH
Sbjct: 121 VLLSYQSSFH 127

BLAST of CSPI02G13120 vs. NCBI nr
Match: PON83413.1 (Transmembrane protein [Trema orientale])

HSP 1 Score: 134.4 bits (337), Expect = 6.7e-28
Identity = 74/130 (56.92%), Postives = 93/130 (71.54%), Query Frame = 0

Query: 1   MDCFYPKRRGPEWKRGLTGTGQTLSSVSPPPIQLLIIFGIVIVLLWFSQFNAGHEAQLNR 60
           M+ FYPKRRGP+WK+G   T QT++S+S P   LL IF IV+VLLW SQ+  G++AQL +
Sbjct: 1   MEWFYPKRRGPQWKQG--WTSQTVASISAPSFHLLAIFSIVMVLLWLSQY-TGYKAQLQQ 60

Query: 61  ---SFQLFLFLLPIFVIFFMYSYSSSRLINFGFR--RSPTGLAQRGASPWTIAILVLLIL 120
              +FQLF+FLLP+ +I FM SYSSS L NF FR  R  T +   G+SPW +A  V L+L
Sbjct: 61  TAVNFQLFMFLLPVLLIVFMASYSSSGLFNFRFRRPRPDTPMQLAGSSPWGVAFFVALLL 120

Query: 121 VLLYYQSSFH 126
           VLL YQSSFH
Sbjct: 121 VLLSYQSSFH 127

BLAST of CSPI02G13120 vs. TAIR 10
Match: AT5G42146.1 (unknown protein; Has 98 Blast hits to 98 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 98; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 59.3 bits (142), Expect = 2.6e-09
Identity = 42/132 (31.82%), Postives = 67/132 (50.76%), Query Frame = 0

Query: 1   MDCFYPKRRGPEWKRGLTGTGQTLSSVSPPPIQLLIIFGIVIVLLWFSQF-NAGHEAQLN 60
           M  F  +RRG  WKRG     +TL + S PP+ LL +  I+ +LL+ S +    +E +  
Sbjct: 1   MGWFIKERRGGSWKRG--WLEETLLTSSAPPLTLLALLAIISLLLFLSSYPRYKYEVEKT 60

Query: 61  -RSFQLFLFLLPIFVIFFMYSYSSSRLINFGFRRS-----PTGLAQRGASPWTIAILVLL 120
             + +LF+  LPI  +F + S S    + F    S        L   G  PW + ++++L
Sbjct: 61  AANLKLFMLFLPILFVFVLVSLSFVHRLLFKSSYSVRANQAKSLFGEGNFPWGVLLMLIL 120

Query: 121 ILVLLYYQSSFH 126
           +L+L+  QS FH
Sbjct: 121 LLLLVSKQSYFH 130

BLAST of CSPI02G13120 vs. TAIR 10
Match: AT2G21180.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G19875.1); Has 124 Blast hits to 124 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 124; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 48.5 bits (114), Expect = 4.5e-06
Identity = 39/114 (34.21%), Postives = 58/114 (50.88%), Query Frame = 0

Query: 26  SVSPPPIQLLIIFGIVIVLLWFSQFNAGHEAQLNRSFQL--FLFLLPIFVIF-------- 85
           S+SP P  +L+I  +  V L  S + +  EA  +   Q+   L L+P+F+I         
Sbjct: 15  SLSPLPYPVLLILAVASVFLLSSWYFSLEEAAESAEEQINFALLLIPLFLIVLVRWLSSM 74

Query: 86  -----FMYSYSSSRLINFGFRRSPTGLAQRGASPWTIAILVLLILVLLYYQSSF 125
                 +  +SSSR   +    SP      G+SPW +A L++L+LVLL YQSSF
Sbjct: 75  ENPDALLGMFSSSRRTTY---VSPGAGGDGGSSPWGVAALIVLLLVLLQYQSSF 125

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0LLH83.7e-64100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G263890 PE=4 SV=1[more]
A0A2P5C2331.9e-2857.69Transmembrane protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_190300 PE=4... [more]
A0A2P5ED063.3e-2856.92Transmembrane protein OS=Trema orientale OX=63057 GN=TorRG33x02_208280 PE=4 SV=1[more]
W9QKI25.6e-2856.06Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_024950 PE=4 SV=1[more]
B9H1487.2e-2857.69Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_004G167800 PE=4 ... [more]
Match NameE-valueIdentityDescription
KGN61894.17.6e-64100.00hypothetical protein Csa_005908 [Cucumis sativus][more]
KAG7010871.11.4e-3065.12hypothetical protein SDJN02_27669, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAF3434655.13.2e-3062.02hypothetical protein FNV43_RR21740 [Rhamnella rubrinervis][more]
PON55102.13.9e-2857.69Transmembrane protein [Parasponia andersonii][more]
PON83413.16.7e-2856.92Transmembrane protein [Trema orientale][more]
Match NameE-valueIdentityDescription
AT5G42146.12.6e-0931.82unknown protein; Has 98 Blast hits to 98 proteins in 12 species: Archae - 0; Bac... [more]
AT2G21180.14.5e-0634.21unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR33306:SF21EXPRESSED PROTEINcoord: 1..125
NoneNo IPR availablePANTHERPTHR33306EXPRESSED PROTEIN-RELATED-RELATEDcoord: 1..125

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G13120.1CSPI02G13120.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016853 isomerase activity