CSPI02G12590 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G12590
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCysteine protease
LocationChr2: 12808342 .. 12810864 (+)
RNA-Seq ExpressionCSPI02G12590
SyntenyCSPI02G12590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAAGAATAAGCATAAAAGGGTATTCTTTTGATACTTTCATTCATAGTTCTTCTTCTTCTCCATTTTTCTCACCATCGATTCTTAGCGGTGAAAATGGTGAAGTGTTTCTCTCTCATCGTCGTTCTCTCTCTCCTCGCAGCATCGGCGATCGGCAGCGAGGTCATTTCCGGAGAATCGGATGGAGATTTCATAATCCGTCAGGTTGTGGATGACGGAGGAGTCAACGAAGGAAGTAACGGCGACGATCTGCTACTCGGAGCCGACCATCACTTTTCGGTCTTTAAGCAAAAGTTTGGGAAGTCGTATGCTTCCAAGGAGGAGCATGATCACCGATTCAGGGTATTCAAAGCCAATCTGAAGCGAGCTCAACGCCACCAGGCTCTTGATCCCTCTGCTACTCATGGCGTCACTCAGTTCTCTGATTTGACTCCTTCGGAGTTCCGAAGATCGTTTCTAGGGCTTAGAAGTCGTCGTCTTGGACTCCCTGCAGATGCTAATAAGGCCCCTATTCTTCCTACTGATGGTCTTCCGACTGATTTCGATTGGAGAGATAAAGGTGCTGTCTCGGAAGTCAAGAATCAGGTTTATGTCTGCTACTTTGAACTCGGGTCTCCGTATATTTTGTTTCTTATGTTAGTGATGGCATCATATTAATTCTTTTAGGAACAAGTAACTTTTATTTATTGAACCGAGGTTTTGATCGTTGTGATTGTTTCTGTTTTTGTTTTCTTATAAGGGTTCGTGCGGATCCTGCTGGAGTTTCAGTGCAACAGGTGCTCTTGAAGGCGCGAACTTCCTTGCTACCGGAAAGCTTGTTAGCCTTAGCGAACAACAGCTTGTAGATTGTGATCACGAGGTTTGAATTTTTTTACTTGTCTTTGATTTGCTCTTCTGTAGTGAATTTGCAGTTTTTTCGTGTAGAAAATTATGATCATTTATAGTTTTCTATTGGGTGGGATATGTTCTTCGCACATGATTCCAGTGTTCTAGAAAGCAAAAAACCTATGTACGACTCTTGCTTATGTTGATGGTTTTCCACTATGAGATGCTGTGAATATGTTTACCACTACTGTTGCAAAATATGTGACAATTCATGAATCTTTGCGTGTGTTTAGTATATCGTATGTAAATAGAAGATCTGTACTAGCAAATTTGGCAGACTCGATAAGGATTAAAGTGCACGTTTTGGCCTTGCAGGTTGCTTGAACTACTTGATAAAGAACTTTTCAGTACCCTATGAATGATTTGGTTCATTTGAACTTGTGGGAATTAGGAATATGTACTTTTGATGTTGCTTATTGTTTATGATCATTTGTGGAGTTATAAGTTTCTGTTTGTCAATTATGTGTCAGTTTAGTGATTCTTGCATATGTAGCATTCATGTCTATCTGAGCACTTAGGAGGGTGTTTTCTCTTACTTTCATGCAAAATTAAAGCACATCTATACTTTTTCCAGTGTGATCCAGAAGAAAAAGGTTCCTGTGATTCTGGCTGTAATGGAGGGCTAATGAATAGTGCATTCGAGTACACATTGAAATCCGGTGGACTTATGAAAGAGCAAGACTATCCATACACTGGTACAGATCGAGGAACCTGCAAATTTGACAAGAGCAAAATTGCTGCATCAGTGGCCAACTTTAGTGTTGTCTCCCTTGATGAAGAACAAATTGCTGCCAACCTCGTGAAGAATGGCCCTCTTGCAGGTATTACACTCAGCCATCCTTTCTTAAATCCCATTATTTTCTTTAGCCTAGCATAGTCTGACTGCTTACTTTCTTCTTAAATGCAGTTGCTATCAACGCTGTGTTCATGCAAACATACATAAAAGGGGTTTCTTGCCCTTACATTTGCTCAAAGCATTTGGATCATGGAGTTCTGTTGGTTGGTTATGGATCTGATGGATATGCTCCCATCCGGTTGAAAGATAAACCATACTGGATCATTAAGAACTCTTGGGGAGCCAACTGGGGGGAGAATGGATACTACAAGATCTGTAGGGGTCGCAATATTTGTGGAGTGGATTCCATGGTGTCGACAGTCGCTGCAGTTCATACCGCTGCAAACTAAAATCATTCTCACATCCATAATTCCTGTATATATTGCATGTGCTGTGGCAGGAAGTTGTCAAGTGCCATTTCAAGCATAGGATGTACCTCTTAAAAATGGAATTTAAACTCTGCTTTGTGTTGGTTCGGAAACTTGATCATAAGTAGCTTTATATATGCATACTTCATATATTTAGTTTAAGTTATTGTGGCGACGAAATGCTTGTAAGCAAAAAGCAAGAATCTGTTTATGGATTATGGATTGGTTCACCTACTCATTTTTGATATGGTGATGCCAATGAAAAAAATATCCCAATGTAAATGTCAAACTTTGATAGAGAATACTTGCTTTTCTGATGTTTTATCTCAATCTTTTCTAATGCTAAATGTCAACAATGAGATAATGATAATTAGAATCTTTGTTGTTTAAGCTTACTTAAAGTAAAACCATTTGGAAACATTCATTGTTTTCTGTTT

mRNA sequence

TGAAGAATAAGCATAAAAGGGTATTCTTTTGATACTTTCATTCATAGTTCTTCTTCTTCTCCATTTTTCTCACCATCGATTCTTAGCGGTGAAAATGGTGAAGTGTTTCTCTCTCATCGTCGTTCTCTCTCTCCTCGCAGCATCGGCGATCGGCAGCGAGGTCATTTCCGGAGAATCGGATGGAGATTTCATAATCCGTCAGGTTGTGGATGACGGAGGAGTCAACGAAGGAAGTAACGGCGACGATCTGCTACTCGGAGCCGACCATCACTTTTCGGTCTTTAAGCAAAAGTTTGGGAAGTCGTATGCTTCCAAGGAGGAGCATGATCACCGATTCAGGGTATTCAAAGCCAATCTGAAGCGAGCTCAACGCCACCAGGCTCTTGATCCCTCTGCTACTCATGGCGTCACTCAGTTCTCTGATTTGACTCCTTCGGAGTTCCGAAGATCGTTTCTAGGGCTTAGAAGTCGTCGTCTTGGACTCCCTGCAGATGCTAATAAGGCCCCTATTCTTCCTACTGATGGTCTTCCGACTGATTTCGATTGGAGAGATAAAGGTGCTGTCTCGGAAGTCAAGAATCAGGGTTCGTGCGGATCCTGCTGGAGTTTCAGTGCAACAGGTGCTCTTGAAGGCGCGAACTTCCTTGCTACCGGAAAGCTTGTTAGCCTTAGCGAACAACAGCTTGTAGATTGTGATCACGAGTGTGATCCAGAAGAAAAAGGTTCCTGTGATTCTGGCTGTAATGGAGGGCTAATGAATAGTGCATTCGAGTACACATTGAAATCCGGTGGACTTATGAAAGAGCAAGACTATCCATACACTGGTACAGATCGAGGAACCTGCAAATTTGACAAGAGCAAAATTGCTGCATCAGTGGCCAACTTTAGTGTTGTCTCCCTTGATGAAGAACAAATTGCTGCCAACCTCGTGAAGAATGGCCCTCTTGCAGTTGCTATCAACGCTGTGTTCATGCAAACATACATAAAAGGGGTTTCTTGCCCTTACATTTGCTCAAAGCATTTGGATCATGGAGTTCTGTTGGTTGGTTATGGATCTGATGGATATGCTCCCATCCGGTTGAAAGATAAACCATACTGGATCATTAAGAACTCTTGGGGAGCCAACTGGGGGGAGAATGGATACTACAAGATCTGTAGGGGTCGCAATATTTGTGGAGTGGATTCCATGGTGTCGACAGTCGCTGCAGTTCATACCGCTGCAAACTAAAATCATTCTCACATCCATAATTCCTGTATATATTGCATGTGCTGTGGCAGGAAGTTGTCAAGTGCCATTTCAAGCATAGGATGTACCTCTTAAAAATGGAATTTAAACTCTGCTTTGTGTTGGTTCGGAAACTTGATCATAAGTAGCTTTATATATGCATACTTCATATATTTAGTTTAAGTTATTGTGGCGACGAAATGCTTGTAAGCAAAAAGCAAGAATCTGTTTATGGATTATGGATTGGTTCACCTACTCATTTTTGATATGGTGATGCCAATGAAAAAAATATCCCAATGTAAATGTCAAACTTTGATAGAGAATACTTGCTTTTCTGATGTTTTATCTCAATCTTTTCTAATGCTAAATGTCAACAATGAGATAATGATAATTAGAATCTTTGTTGTTTAAGCTTACTTAAAGTAAAACCATTTGGAAACATTCATTGTTTTCTGTTT

Coding sequence (CDS)

ATGGTGAAGTGTTTCTCTCTCATCGTCGTTCTCTCTCTCCTCGCAGCATCGGCGATCGGCAGCGAGGTCATTTCCGGAGAATCGGATGGAGATTTCATAATCCGTCAGGTTGTGGATGACGGAGGAGTCAACGAAGGAAGTAACGGCGACGATCTGCTACTCGGAGCCGACCATCACTTTTCGGTCTTTAAGCAAAAGTTTGGGAAGTCGTATGCTTCCAAGGAGGAGCATGATCACCGATTCAGGGTATTCAAAGCCAATCTGAAGCGAGCTCAACGCCACCAGGCTCTTGATCCCTCTGCTACTCATGGCGTCACTCAGTTCTCTGATTTGACTCCTTCGGAGTTCCGAAGATCGTTTCTAGGGCTTAGAAGTCGTCGTCTTGGACTCCCTGCAGATGCTAATAAGGCCCCTATTCTTCCTACTGATGGTCTTCCGACTGATTTCGATTGGAGAGATAAAGGTGCTGTCTCGGAAGTCAAGAATCAGGGTTCGTGCGGATCCTGCTGGAGTTTCAGTGCAACAGGTGCTCTTGAAGGCGCGAACTTCCTTGCTACCGGAAAGCTTGTTAGCCTTAGCGAACAACAGCTTGTAGATTGTGATCACGAGTGTGATCCAGAAGAAAAAGGTTCCTGTGATTCTGGCTGTAATGGAGGGCTAATGAATAGTGCATTCGAGTACACATTGAAATCCGGTGGACTTATGAAAGAGCAAGACTATCCATACACTGGTACAGATCGAGGAACCTGCAAATTTGACAAGAGCAAAATTGCTGCATCAGTGGCCAACTTTAGTGTTGTCTCCCTTGATGAAGAACAAATTGCTGCCAACCTCGTGAAGAATGGCCCTCTTGCAGTTGCTATCAACGCTGTGTTCATGCAAACATACATAAAAGGGGTTTCTTGCCCTTACATTTGCTCAAAGCATTTGGATCATGGAGTTCTGTTGGTTGGTTATGGATCTGATGGATATGCTCCCATCCGGTTGAAAGATAAACCATACTGGATCATTAAGAACTCTTGGGGAGCCAACTGGGGGGAGAATGGATACTACAAGATCTGTAGGGGTCGCAATATTTGTGGAGTGGATTCCATGGTGTCGACAGTCGCTGCAGTTCATACCGCTGCAAACTAA

Protein sequence

MVKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHFSVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSFLGLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEGANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDYPYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGVSCPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNICGVDSMVSTVAAVHTAAN*
Homology
BLAST of CSPI02G12590 vs. ExPASy Swiss-Prot
Match: P43296 (Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 2.2e-157
Identity = 280/377 (74.27%), Postives = 313/377 (83.02%), Query Frame = 0

Query: 2   VKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHFS 61
           +K +  + VLS    S   S+V  G+   D +IRQVV  GG       +  +L ++ HFS
Sbjct: 4   LKLYFSVFVLSFFIVSVSSSDVNDGD---DLVIRQVV--GG------AEPQVLTSEDHFS 63

Query: 62  VFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSFL 121
           +FK+KFGK YAS EEHD+RF VFKANL+RA+RHQ LDPSATHGVTQFSDLT SEFR+  L
Sbjct: 64  LFKRKFGKVYASNEEHDYRFSVFKANLRRARRHQKLDPSATHGVTQFSDLTRSEFRKKHL 123

Query: 122 GLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEGA 181
           G+RS    LP DANKAPILPT+ LP DFDWRD GAV+ VKNQGSCGSCWSFSATGALEGA
Sbjct: 124 GVRS-GFKLPKDANKAPILPTENLPEDFDWRDHGAVTPVKNQGSCGSCWSFSATGALEGA 183

Query: 182 NFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDYP 241
           NFLATGKLVSLSEQQLVDCDHECDPEE  SCDSGCNGGLMNSAFEYTLK+GGLMKE+DYP
Sbjct: 184 NFLATGKLVSLSEQQLVDCDHECDPEEADSCDSGCNGGLMNSAFEYTLKTGGLMKEEDYP 243

Query: 242 YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGVS 301
           YTG D  TCK DKSKI ASV+NFSV+S+DEEQIAANLVKNGPLAVAINA +MQTYI GVS
Sbjct: 244 YTGKDGKTCKLDKSKIVASVSNFSVISIDEEQIAANLVKNGPLAVAINAGYMQTYIGGVS 303

Query: 302 CPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNICG 361
           CPYIC++ L+HGVLLVGYG+ GYAP R K+KPYWIIKNSWG  WGENG+YKIC+GRNICG
Sbjct: 304 CPYICTRRLNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFYKICKGRNICG 363

Query: 362 VDSMVSTVAA-VHTAAN 378
           VDSMVSTVAA V T A+
Sbjct: 364 VDSMVSTVAATVSTTAH 368

BLAST of CSPI02G12590 vs. ExPASy Swiss-Prot
Match: Q9SUL1 (Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 6.5e-154
Identity = 267/374 (71.39%), Postives = 311/374 (83.16%), Query Frame = 0

Query: 5   FSLIVVLSLLAASAIGSEVISGESDGDFI--IRQVVDDGGVNEGSNGDDLLLGADHHFSV 64
           F  ++  +LLA S +GS VISGE    F+  IRQVV +         D+ LL A+HHF++
Sbjct: 6   FFFLIAATLLAGS-LGSTVISGEVTDGFVNPIRQVVPE-------ENDEQLLNAEHHFTL 65

Query: 65  FKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSFLG 124
           FK K+ K+YA++ EHDHRFRVFKANL+RA+R+Q LDPSA HGVTQFSDLTP EFRR FLG
Sbjct: 66  FKSKYEKTYATQVEHDHRFRVFKANLRRARRNQLLDPSAVHGVTQFSDLTPKEFRRKFLG 125

Query: 125 LRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEGAN 184
           L+ R   LP D   APILPT  LPT+FDWR++GAV+ VKNQG CGSCWSFSA GALEGA+
Sbjct: 126 LKRRGFRLPTDTQTAPILPTSDLPTEFDWREQGAVTPVKNQGMCGSCWSFSAIGALEGAH 185

Query: 185 FLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDYPY 244
           FLAT +LVSLSEQQLVDCDHECDP +  SCDSGC+GGLMN+AFEY LK+GGLMKE+DYPY
Sbjct: 186 FLATKELVSLSEQQLVDCDHECDPAQANSCDSGCSGGLMNNAFEYALKAGGLMKEEDYPY 245

Query: 245 TGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGVSC 304
           TG D   CKFDKSKI ASV+NFSVVS DE+QIAANLV++GPLA+AINA++MQTYI GVSC
Sbjct: 246 TGRDHTACKFDKSKIVASVSNFSVVSSDEDQIAANLVQHGPLAIAINAMWMQTYIGGVSC 305

Query: 305 PYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRG-RNICG 364
           PY+CSK  DHGVLLVG+GS GYAPIRLK+KPYWIIKNSWGA WGE+GYYKICRG  N+CG
Sbjct: 306 PYVCSKSQDHGVLLVGFGSSGYAPIRLKEKPYWIIKNSWGAMWGEHGYYKICRGPHNMCG 365

Query: 365 VDSMVSTVAAVHTA 376
           +D+MVSTVAAVHT+
Sbjct: 366 MDTMVSTVAAVHTS 371

BLAST of CSPI02G12590 vs. ExPASy Swiss-Prot
Match: P25804 (Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 6.7e-151
Identity = 260/366 (71.04%), Postives = 306/366 (83.61%), Query Frame = 0

Query: 10  VLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHFSVFKQKFGK 69
           + +L   +A+ + V    ++ DFIIRQVVD        N +D LL A+HHF+ FK KF K
Sbjct: 6   LFALFLFAAVATAVTDDTNNDDFIIRQVVD--------NEEDHLLNAEHHFTSFKSKFSK 65

Query: 70  SYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSFLGLRSRRLG 129
           SYA+KEEHD+RF VFK+NL +A+ HQ  DP+A HG+T+FSDLT SEFRR FLGL+ +RL 
Sbjct: 66  SYATKEEHDYRFGVFKSNLIKAKLHQNRDPTAEHGITKFSDLTASEFRRQFLGLK-KRLR 125

Query: 130 LPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEGANFLATGKL 189
           LPA A KAPILPT  LP DFDWR+KGAV+ VK+QGSCGSCW+FS TGALEGA++LATGKL
Sbjct: 126 LPAHAQKAPILPTTNLPEDFDWREKGAVTPVKDQGSCGSCWAFSTTGALEGAHYLATGKL 185

Query: 190 VSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDYPYTGTDRGT 249
           VSLSEQQLVDCDH CDPE+ GSCDSGCNGGLMN+AFEY L+SGG+++E+DY YTG D G+
Sbjct: 186 VSLSEQQLVDCDHVCDPEQAGSCDSGCNGGLMNNAFEYLLESGGVVQEKDYAYTGRD-GS 245

Query: 250 CKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGVSCPYICSK- 309
           CKFDKSK+ ASV+NFSVV+LDE+QIAANLVKNGPLAVAINA +MQTY+ GVSCPY+C+K 
Sbjct: 246 CKFDKSKVVASVSNFSVVTLDEDQIAANLVKNGPLAVAINAAWMQTYMSGVSCPYVCAKS 305

Query: 310 HLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNICGVDSMVST 369
            LDHGVLLVG+G   YAPIRLK+KPYWIIKNSWG NWGE GYYKICRGRN+CGVDSMVST
Sbjct: 306 RLDHGVLLVGFGKGAYAPIRLKEKPYWIIKNSWGQNWGEQGYYKICRGRNVCGVDSMVST 361

Query: 370 VAAVHT 375
           VAA  +
Sbjct: 366 VAAAQS 361

BLAST of CSPI02G12590 vs. ExPASy Swiss-Prot
Match: P43295 (Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 SV=2)

HSP 1 Score: 534.3 bits (1375), Expect = 1.1e-150
Identity = 260/367 (70.84%), Postives = 304/367 (82.83%), Query Frame = 0

Query: 5   FSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHFSVFK 64
           FS+ ++   ++ S  G E        D +IRQVVD+         +  +L ++ HF++FK
Sbjct: 9   FSVSLIFVFVSVSVCGDE--------DVLIRQVVDE--------TEPKVLSSEDHFTLFK 68

Query: 65  QKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSFLGLR 124
           +KFGK Y S EEH +RF VFKANL RA RHQ +DPSA HGVTQFSDLT SEFRR  LG++
Sbjct: 69  KKFGKVYGSIEEHYYRFSVFKANLLRAMRHQKMDPSARHGVTQFSDLTRSEFRRKHLGVK 128

Query: 125 SRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEGANFL 184
                LP DAN+APILPT  LP +FDWRD+GAV+ VKNQGSCGSCWSFS TGALEGA+FL
Sbjct: 129 G-GFKLPKDANQAPILPTQNLPEEFDWRDRGAVTPVKNQGSCGSCWSFSTTGALEGAHFL 188

Query: 185 ATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDYPYTG 244
           ATGKLVSLSEQQLVDCDHECDPEE+GSCDSGCNGGLMNSAFEYTLK+GGLM+E+DYPYTG
Sbjct: 189 ATGKLVSLSEQQLVDCDHECDPEEEGSCDSGCNGGLMNSAFEYTLKTGGLMREKDYPYTG 248

Query: 245 TDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGVSCPY 304
           TD G+CK D+SKI ASV+NFSVVS++E+QIAANL+KNGPLAVAINA +MQTYI GVSCPY
Sbjct: 249 TDGGSCKLDRSKIVASVSNFSVVSINEDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPY 308

Query: 305 ICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNICGVDS 364
           ICS+ L+HGVLLVGYGS G++  RLK+KPYWIIKNSWG +WGENG+YKIC+GRNICGVDS
Sbjct: 309 ICSRRLNHGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGENGFYKICKGRNICGVDS 358

Query: 365 MVSTVAA 372
           +VSTVAA
Sbjct: 369 LVSTVAA 358

BLAST of CSPI02G12590 vs. ExPASy Swiss-Prot
Match: Q10716 (Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 8.0e-144
Identity = 255/376 (67.82%), Postives = 298/376 (79.26%), Query Frame = 0

Query: 7   LIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHFSVFKQK 66
           ++++LSL +A+A+ + V       D +IRQVV       G + +DL L A+ HF  F Q+
Sbjct: 5   VLLLLSLASAAAVAAAV----DAEDPLIRQVV------PGGDDNDLELNAESHFLSFVQR 64

Query: 67  FGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSFLGLRSR 126
           FGKSY   +EH +R  VFK NL+RA+RHQ LDPSA HGVT+FSDLTP+EFRR++LGLR  
Sbjct: 65  FGKSYKDADEHAYRLSVFKDNLRRARRHQLLDPSAEHGVTKFSDLTPAEFRRTYLGLRKS 124

Query: 127 RLGLPAD----ANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEGAN 186
           R  L  +    A++AP+LPTDGLP DFDWRD GAV  VKNQGSCGSCWSFSA+GALEGA+
Sbjct: 125 RRALLRELGESAHEAPVLPTDGLPDDFDWRDHGAVGPVKNQGSCGSCWSFSASGALEGAH 184

Query: 187 FLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDYPY 246
           +LATGKL  LSEQQ VDCDHECD  E  SCDSGCNGGLM +AF Y  K+GGL  E+DYPY
Sbjct: 185 YLATGKLEVLSEQQFVDCDHECDSSEPDSCDSGCNGGLMTTAFSYLQKAGGLESEKDYPY 244

Query: 247 TGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGVSC 306
           TG+D G CKFDKSKI ASV NFSVVS+DE QI+ANL+K+GPLA+ INA +MQTYI GVSC
Sbjct: 245 TGSD-GKCKFDKSKIVASVQNFSVVSVDEAQISANLIKHGPLAIGINAAYMQTYIGGVSC 304

Query: 307 PYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRG---RNI 366
           PYIC +HLDHGVLLVGYG+ G+APIRLKDKPYWIIKNSWG NWGENGYYKICRG   RN 
Sbjct: 305 PYICGRHLDHGVLLVGYGASGFAPIRLKDKPYWIIKNSWGENWGENGYYKICRGSNVRNK 364

Query: 367 CGVDSMVSTVAAVHTA 376
           CGVDSMVSTV+AVH +
Sbjct: 365 CGVDSMVSTVSAVHAS 369

BLAST of CSPI02G12590 vs. ExPASy TrEMBL
Match: A0A0A0LPD0 (Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_2G249900 PE=3 SV=1)

HSP 1 Score: 767.7 bits (1981), Expect = 2.3e-218
Identity = 377/377 (100.00%), Postives = 377/377 (100.00%), Query Frame = 0

Query: 1   MVKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHF 60
           MVKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHF
Sbjct: 1   MVKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHF 60

Query: 61  SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120
           SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 121 LGLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEG 180
           LGLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEG
Sbjct: 121 LGLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEG 180

Query: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY 240
           ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY
Sbjct: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY 240

Query: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGV 300
           PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGV
Sbjct: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGV 300

Query: 301 SCPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNIC 360
           SCPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNIC
Sbjct: 301 SCPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNIC 360

Query: 361 GVDSMVSTVAAVHTAAN 378
           GVDSMVSTVAAVHTAAN
Sbjct: 361 GVDSMVSTVAAVHTAAN 377

BLAST of CSPI02G12590 vs. ExPASy TrEMBL
Match: A0A5D3BWN5 (Cysteine proteinase RD19a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold280G00440 PE=3 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 1.9e-212
Identity = 364/377 (96.55%), Postives = 371/377 (98.41%), Query Frame = 0

Query: 1   MVKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHF 60
           MVKCFSLI+ LSLLAASAIG EVISGESDGDFIIRQVVDDGGVN  SNGDDLLLGA+HHF
Sbjct: 1   MVKCFSLIIALSLLAASAIGKEVISGESDGDFIIRQVVDDGGVNGASNGDDLLLGAEHHF 60

Query: 61  SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120
           SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 121 LGLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEG 180
           LGLRSRRLGLP DANKAPILPTDGLP DFDWRDKGAV+EVKNQGSCGSCWSFSATGALEG
Sbjct: 121 LGLRSRRLGLPEDANKAPILPTDGLPADFDWRDKGAVTEVKNQGSCGSCWSFSATGALEG 180

Query: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY 240
           ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY
Sbjct: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY 240

Query: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGV 300
           PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYI+GV
Sbjct: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIRGV 300

Query: 301 SCPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNIC 360
           SCPYICS+HLDHGVLLVGYGS+GYAPIR+KDKPYWIIKNSWGANWGENGYYKICRGRNIC
Sbjct: 301 SCPYICSRHLDHGVLLVGYGSEGYAPIRMKDKPYWIIKNSWGANWGENGYYKICRGRNIC 360

Query: 361 GVDSMVSTVAAVHTAAN 378
           GVDSMVSTVAAVHTAAN
Sbjct: 361 GVDSMVSTVAAVHTAAN 377

BLAST of CSPI02G12590 vs. ExPASy TrEMBL
Match: A0A1S3CE63 (cysteine proteinase RD19a-like OS=Cucumis melo OX=3656 GN=LOC103499710 PE=3 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 1.9e-212
Identity = 364/377 (96.55%), Postives = 371/377 (98.41%), Query Frame = 0

Query: 1   MVKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHF 60
           MVKCFSLI+ LSLLAASAIG EVISGESDGDFIIRQVVDDGGVN  SNGDDLLLGA+HHF
Sbjct: 1   MVKCFSLIIALSLLAASAIGKEVISGESDGDFIIRQVVDDGGVNGASNGDDLLLGAEHHF 60

Query: 61  SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120
           SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 121 LGLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEG 180
           LGLRSRRLGLP DANKAPILPTDGLP DFDWRDKGAV+EVKNQGSCGSCWSFSATGALEG
Sbjct: 121 LGLRSRRLGLPEDANKAPILPTDGLPADFDWRDKGAVTEVKNQGSCGSCWSFSATGALEG 180

Query: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY 240
           ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY
Sbjct: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY 240

Query: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGV 300
           PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYI+GV
Sbjct: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIRGV 300

Query: 301 SCPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNIC 360
           SCPYICS+HLDHGVLLVGYGS+GYAPIR+KDKPYWIIKNSWGANWGENGYYKICRGRNIC
Sbjct: 301 SCPYICSRHLDHGVLLVGYGSEGYAPIRMKDKPYWIIKNSWGANWGENGYYKICRGRNIC 360

Query: 361 GVDSMVSTVAAVHTAAN 378
           GVDSMVSTVAAVHTAAN
Sbjct: 361 GVDSMVSTVAAVHTAAN 377

BLAST of CSPI02G12590 vs. ExPASy TrEMBL
Match: A0A7R6B0V1 (Cysteine proteinase 2 OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 731.5 bits (1887), Expect = 1.8e-207
Identity = 355/377 (94.16%), Postives = 365/377 (96.82%), Query Frame = 0

Query: 1   MVKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHF 60
           MVKCFSLIV LSLLA SAIG+EV SGESDGDF+IRQVVDDGGVN GSNGDDLLLGA+HHF
Sbjct: 1   MVKCFSLIVALSLLAVSAIGAEVFSGESDGDFVIRQVVDDGGVNGGSNGDDLLLGAEHHF 60

Query: 61  SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120
           S+FK+KFGKSYASKEEHDHRFRVFKANL+RAQRHQALDPSATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SLFKRKFGKSYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 121 LGLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEG 180
           LGLR RRLGLPADANKAPILPTDGLPTDFDWRD GAV+EVKNQGSCGSCWSFS TGALEG
Sbjct: 121 LGLRGRRLGLPADANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG 180

Query: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY 240
           ANFLATGKLVSLSEQQLVDCDHECDPEEK SCDSGCNGGLMNSAFEYTLK+GGLMKE DY
Sbjct: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMKENDY 240

Query: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGV 300
           PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLA+AINAVFMQTYI GV
Sbjct: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGV 300

Query: 301 SCPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNIC 360
           SCPYICSKHLDHGVLLVGYGS  YAPIRLK+KPYWIIKNSWGANWGENGYYKICRGRNIC
Sbjct: 301 SCPYICSKHLDHGVLLVGYGSGAYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNIC 360

Query: 361 GVDSMVSTVAAVHTAAN 378
           GVDSMVSTVAAVHTAAN
Sbjct: 361 GVDSMVSTVAAVHTAAN 377

BLAST of CSPI02G12590 vs. ExPASy TrEMBL
Match: A0A6J1FW38 (cysteine protease RD19A-like OS=Cucurbita moschata OX=3662 GN=LOC111448673 PE=3 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 1.2e-198
Identity = 340/377 (90.19%), Postives = 361/377 (95.76%), Query Frame = 0

Query: 1   MVKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHF 60
           MVK FS +VVLSL+ A+AIGSEV+SGESDGDFIIRQVVDDGGVN GSNGDDLLLGA+HHF
Sbjct: 1   MVKGFSFLVVLSLVVATAIGSEVLSGESDGDFIIRQVVDDGGVNRGSNGDDLLLGAEHHF 60

Query: 61  SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120
           S+FK++FGKSYAS EEHDHRFR+FKANL+RA+RHQALDPSATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 121 LGLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEG 180
           LGLR  RL LPADANKAPILPTD LPTDFDWRD GAV+EVKNQGSCGSCWSFS TGALEG
Sbjct: 121 LGLRD-RLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG 180

Query: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY 240
           ANFLATGKLVSLSEQQLVDCDHECDPEEK SCDSGCNGGLMNSAFEYTLK+GGLM+E+DY
Sbjct: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDY 240

Query: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGV 300
           PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANL+KNGPLAVAINAV+MQTY+ GV
Sbjct: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGV 300

Query: 301 SCPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNIC 360
           SCPYICSK LDHGVLLVGYGS GYAPIR+K+KPYWIIKNSWGA WGENGYYKICRGRN+C
Sbjct: 301 SCPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGYYKICRGRNVC 360

Query: 361 GVDSMVSTVAAVHTAAN 378
           GVDSMVSTVAAVHTAAN
Sbjct: 361 GVDSMVSTVAAVHTAAN 376

BLAST of CSPI02G12590 vs. NCBI nr
Match: XP_004150061.1 (cysteine protease RD19A [Cucumis sativus] >KGN61836.1 hypothetical protein Csa_006176 [Cucumis sativus])

HSP 1 Score: 767.7 bits (1981), Expect = 4.7e-218
Identity = 377/377 (100.00%), Postives = 377/377 (100.00%), Query Frame = 0

Query: 1   MVKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHF 60
           MVKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHF
Sbjct: 1   MVKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHF 60

Query: 61  SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120
           SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 121 LGLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEG 180
           LGLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEG
Sbjct: 121 LGLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEG 180

Query: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY 240
           ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY
Sbjct: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY 240

Query: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGV 300
           PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGV
Sbjct: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGV 300

Query: 301 SCPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNIC 360
           SCPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNIC
Sbjct: 301 SCPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNIC 360

Query: 361 GVDSMVSTVAAVHTAAN 378
           GVDSMVSTVAAVHTAAN
Sbjct: 361 GVDSMVSTVAAVHTAAN 377

BLAST of CSPI02G12590 vs. NCBI nr
Match: XP_008460996.1 (PREDICTED: cysteine proteinase RD19a-like [Cucumis melo] >KAA0045619.1 cysteine proteinase RD19a-like [Cucumis melo var. makuwa] >TYK02636.1 cysteine proteinase RD19a-like [Cucumis melo var. makuwa])

HSP 1 Score: 748.0 bits (1930), Expect = 3.9e-212
Identity = 364/377 (96.55%), Postives = 371/377 (98.41%), Query Frame = 0

Query: 1   MVKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHF 60
           MVKCFSLI+ LSLLAASAIG EVISGESDGDFIIRQVVDDGGVN  SNGDDLLLGA+HHF
Sbjct: 1   MVKCFSLIIALSLLAASAIGKEVISGESDGDFIIRQVVDDGGVNGASNGDDLLLGAEHHF 60

Query: 61  SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120
           SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 121 LGLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEG 180
           LGLRSRRLGLP DANKAPILPTDGLP DFDWRDKGAV+EVKNQGSCGSCWSFSATGALEG
Sbjct: 121 LGLRSRRLGLPEDANKAPILPTDGLPADFDWRDKGAVTEVKNQGSCGSCWSFSATGALEG 180

Query: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY 240
           ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY
Sbjct: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY 240

Query: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGV 300
           PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYI+GV
Sbjct: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIRGV 300

Query: 301 SCPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNIC 360
           SCPYICS+HLDHGVLLVGYGS+GYAPIR+KDKPYWIIKNSWGANWGENGYYKICRGRNIC
Sbjct: 301 SCPYICSRHLDHGVLLVGYGSEGYAPIRMKDKPYWIIKNSWGANWGENGYYKICRGRNIC 360

Query: 361 GVDSMVSTVAAVHTAAN 378
           GVDSMVSTVAAVHTAAN
Sbjct: 361 GVDSMVSTVAAVHTAAN 377

BLAST of CSPI02G12590 vs. NCBI nr
Match: ARA15252.1 (cysteine proteinase 2 [Citrullus lanatus])

HSP 1 Score: 731.5 bits (1887), Expect = 3.7e-207
Identity = 355/377 (94.16%), Postives = 365/377 (96.82%), Query Frame = 0

Query: 1   MVKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHF 60
           MVKCFSLIV LSLLA SAIG+EV SGESDGDF+IRQVVDDGGVN GSNGDDLLLGA+HHF
Sbjct: 1   MVKCFSLIVALSLLAVSAIGAEVFSGESDGDFVIRQVVDDGGVNGGSNGDDLLLGAEHHF 60

Query: 61  SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120
           S+FK+KFGKSYASKEEHDHRFRVFKANL+RAQRHQALDPSATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SLFKRKFGKSYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 121 LGLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEG 180
           LGLR RRLGLPADANKAPILPTDGLPTDFDWRD GAV+EVKNQGSCGSCWSFS TGALEG
Sbjct: 121 LGLRGRRLGLPADANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG 180

Query: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY 240
           ANFLATGKLVSLSEQQLVDCDHECDPEEK SCDSGCNGGLMNSAFEYTLK+GGLMKE DY
Sbjct: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMKENDY 240

Query: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGV 300
           PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLA+AINAVFMQTYI GV
Sbjct: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGV 300

Query: 301 SCPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNIC 360
           SCPYICSKHLDHGVLLVGYGS  YAPIRLK+KPYWIIKNSWGANWGENGYYKICRGRNIC
Sbjct: 301 SCPYICSKHLDHGVLLVGYGSGAYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNIC 360

Query: 361 GVDSMVSTVAAVHTAAN 378
           GVDSMVSTVAAVHTAAN
Sbjct: 361 GVDSMVSTVAAVHTAAN 377

BLAST of CSPI02G12590 vs. NCBI nr
Match: XP_038901827.1 (cysteine protease RD19A-like [Benincasa hispida])

HSP 1 Score: 721.5 bits (1861), Expect = 3.9e-204
Identity = 348/377 (92.31%), Postives = 364/377 (96.55%), Query Frame = 0

Query: 1   MVKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHF 60
           MVKCFSLIV LSLLA +AIG+EV SGESDGDFIIRQVVDDG VN GSNGDDLLLGA+HHF
Sbjct: 1   MVKCFSLIVALSLLAVAAIGAEVFSGESDGDFIIRQVVDDGVVNGGSNGDDLLLGAEHHF 60

Query: 61  SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120
           S+FK+KFGKSYASKEEHDHRFRVFKANL+RAQRHQALDPSATHGVTQFSDLTP EFR SF
Sbjct: 61  SLFKRKFGKSYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPLEFRSSF 120

Query: 121 LGLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEG 180
           LGLR RRL LPADANKAP+LPTD LPTDFDWRD GAV+EVKNQGSCGSCWSFS TGALEG
Sbjct: 121 LGLRGRRLRLPADANKAPVLPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG 180

Query: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY 240
           ANFLATGKLVSLSEQQLVDCDHECDPEEK SCDSGC+GGLMNSAFEYTLK+GGLMKE+DY
Sbjct: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCSGGLMNSAFEYTLKAGGLMKEKDY 240

Query: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGV 300
           PYTGTDRGTCKFDKSKIAASVANFSV+SLDEEQIAANLVKNGPLA+AINAVFMQTYI+GV
Sbjct: 241 PYTGTDRGTCKFDKSKIAASVANFSVISLDEEQIAANLVKNGPLAIAINAVFMQTYIRGV 300

Query: 301 SCPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNIC 360
           SCPYICSKHLDHGVLLVGYGSDGYAPIR+KDKPYWIIKNSWGANWGENGYYKICRGRN+C
Sbjct: 301 SCPYICSKHLDHGVLLVGYGSDGYAPIRMKDKPYWIIKNSWGANWGENGYYKICRGRNVC 360

Query: 361 GVDSMVSTVAAVHTAAN 378
           GVDSMVSTVAAVHTAAN
Sbjct: 361 GVDSMVSTVAAVHTAAN 377

BLAST of CSPI02G12590 vs. NCBI nr
Match: XP_022944124.1 (cysteine protease RD19A-like [Cucurbita moschata] >KAG7010902.1 Cysteine protease RD19A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 702.2 bits (1811), Expect = 2.4e-198
Identity = 340/377 (90.19%), Postives = 361/377 (95.76%), Query Frame = 0

Query: 1   MVKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHF 60
           MVK FS +VVLSL+ A+AIGSEV+SGESDGDFIIRQVVDDGGVN GSNGDDLLLGA+HHF
Sbjct: 1   MVKGFSFLVVLSLVVATAIGSEVLSGESDGDFIIRQVVDDGGVNRGSNGDDLLLGAEHHF 60

Query: 61  SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120
           S+FK++FGKSYAS EEHDHRFR+FKANL+RA+RHQALDPSATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 121 LGLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEG 180
           LGLR  RL LPADANKAPILPTD LPTDFDWRD GAV+EVKNQGSCGSCWSFS TGALEG
Sbjct: 121 LGLRD-RLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG 180

Query: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY 240
           ANFLATGKLVSLSEQQLVDCDHECDPEEK SCDSGCNGGLMNSAFEYTLK+GGLM+E+DY
Sbjct: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDY 240

Query: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGV 300
           PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANL+KNGPLAVAINAV+MQTY+ GV
Sbjct: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGV 300

Query: 301 SCPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNIC 360
           SCPYICSK LDHGVLLVGYGS GYAPIR+K+KPYWIIKNSWGA WGENGYYKICRGRN+C
Sbjct: 301 SCPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGYYKICRGRNVC 360

Query: 361 GVDSMVSTVAAVHTAAN 378
           GVDSMVSTVAAVHTAAN
Sbjct: 361 GVDSMVSTVAAVHTAAN 376

BLAST of CSPI02G12590 vs. TAIR 10
Match: AT4G39090.1 (Papain family cysteine protease )

HSP 1 Score: 556.6 bits (1433), Expect = 1.5e-158
Identity = 280/377 (74.27%), Postives = 313/377 (83.02%), Query Frame = 0

Query: 2   VKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHFS 61
           +K +  + VLS    S   S+V  G+   D +IRQVV  GG       +  +L ++ HFS
Sbjct: 4   LKLYFSVFVLSFFIVSVSSSDVNDGD---DLVIRQVV--GG------AEPQVLTSEDHFS 63

Query: 62  VFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSFL 121
           +FK+KFGK YAS EEHD+RF VFKANL+RA+RHQ LDPSATHGVTQFSDLT SEFR+  L
Sbjct: 64  LFKRKFGKVYASNEEHDYRFSVFKANLRRARRHQKLDPSATHGVTQFSDLTRSEFRKKHL 123

Query: 122 GLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEGA 181
           G+RS    LP DANKAPILPT+ LP DFDWRD GAV+ VKNQGSCGSCWSFSATGALEGA
Sbjct: 124 GVRS-GFKLPKDANKAPILPTENLPEDFDWRDHGAVTPVKNQGSCGSCWSFSATGALEGA 183

Query: 182 NFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDYP 241
           NFLATGKLVSLSEQQLVDCDHECDPEE  SCDSGCNGGLMNSAFEYTLK+GGLMKE+DYP
Sbjct: 184 NFLATGKLVSLSEQQLVDCDHECDPEEADSCDSGCNGGLMNSAFEYTLKTGGLMKEEDYP 243

Query: 242 YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGVS 301
           YTG D  TCK DKSKI ASV+NFSV+S+DEEQIAANLVKNGPLAVAINA +MQTYI GVS
Sbjct: 244 YTGKDGKTCKLDKSKIVASVSNFSVISIDEEQIAANLVKNGPLAVAINAGYMQTYIGGVS 303

Query: 302 CPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNICG 361
           CPYIC++ L+HGVLLVGYG+ GYAP R K+KPYWIIKNSWG  WGENG+YKIC+GRNICG
Sbjct: 304 CPYICTRRLNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFYKICKGRNICG 363

Query: 362 VDSMVSTVAA-VHTAAN 378
           VDSMVSTVAA V T A+
Sbjct: 364 VDSMVSTVAATVSTTAH 368

BLAST of CSPI02G12590 vs. TAIR 10
Match: AT4G16190.1 (Papain family cysteine protease )

HSP 1 Score: 545.0 bits (1403), Expect = 4.6e-155
Identity = 267/374 (71.39%), Postives = 311/374 (83.16%), Query Frame = 0

Query: 5   FSLIVVLSLLAASAIGSEVISGESDGDFI--IRQVVDDGGVNEGSNGDDLLLGADHHFSV 64
           F  ++  +LLA S +GS VISGE    F+  IRQVV +         D+ LL A+HHF++
Sbjct: 6   FFFLIAATLLAGS-LGSTVISGEVTDGFVNPIRQVVPE-------ENDEQLLNAEHHFTL 65

Query: 65  FKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSFLG 124
           FK K+ K+YA++ EHDHRFRVFKANL+RA+R+Q LDPSA HGVTQFSDLTP EFRR FLG
Sbjct: 66  FKSKYEKTYATQVEHDHRFRVFKANLRRARRNQLLDPSAVHGVTQFSDLTPKEFRRKFLG 125

Query: 125 LRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEGAN 184
           L+ R   LP D   APILPT  LPT+FDWR++GAV+ VKNQG CGSCWSFSA GALEGA+
Sbjct: 126 LKRRGFRLPTDTQTAPILPTSDLPTEFDWREQGAVTPVKNQGMCGSCWSFSAIGALEGAH 185

Query: 185 FLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDYPY 244
           FLAT +LVSLSEQQLVDCDHECDP +  SCDSGC+GGLMN+AFEY LK+GGLMKE+DYPY
Sbjct: 186 FLATKELVSLSEQQLVDCDHECDPAQANSCDSGCSGGLMNNAFEYALKAGGLMKEEDYPY 245

Query: 245 TGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGVSC 304
           TG D   CKFDKSKI ASV+NFSVVS DE+QIAANLV++GPLA+AINA++MQTYI GVSC
Sbjct: 246 TGRDHTACKFDKSKIVASVSNFSVVSSDEDQIAANLVQHGPLAIAINAMWMQTYIGGVSC 305

Query: 305 PYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRG-RNICG 364
           PY+CSK  DHGVLLVG+GS GYAPIRLK+KPYWIIKNSWGA WGE+GYYKICRG  N+CG
Sbjct: 306 PYVCSKSQDHGVLLVGFGSSGYAPIRLKEKPYWIIKNSWGAMWGEHGYYKICRGPHNMCG 365

Query: 365 VDSMVSTVAAVHTA 376
           +D+MVSTVAAVHT+
Sbjct: 366 MDTMVSTVAAVHTS 371

BLAST of CSPI02G12590 vs. TAIR 10
Match: AT2G21430.1 (Papain family cysteine protease )

HSP 1 Score: 534.3 bits (1375), Expect = 8.1e-152
Identity = 260/367 (70.84%), Postives = 304/367 (82.83%), Query Frame = 0

Query: 5   FSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHFSVFK 64
           FS+ ++   ++ S  G E        D +IRQVVD+         +  +L ++ HF++FK
Sbjct: 9   FSVSLIFVFVSVSVCGDE--------DVLIRQVVDE--------TEPKVLSSEDHFTLFK 68

Query: 65  QKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSFLGLR 124
           +KFGK Y S EEH +RF VFKANL RA RHQ +DPSA HGVTQFSDLT SEFRR  LG++
Sbjct: 69  KKFGKVYGSIEEHYYRFSVFKANLLRAMRHQKMDPSARHGVTQFSDLTRSEFRRKHLGVK 128

Query: 125 SRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEGANFL 184
                LP DAN+APILPT  LP +FDWRD+GAV+ VKNQGSCGSCWSFS TGALEGA+FL
Sbjct: 129 G-GFKLPKDANQAPILPTQNLPEEFDWRDRGAVTPVKNQGSCGSCWSFSTTGALEGAHFL 188

Query: 185 ATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDYPYTG 244
           ATGKLVSLSEQQLVDCDHECDPEE+GSCDSGCNGGLMNSAFEYTLK+GGLM+E+DYPYTG
Sbjct: 189 ATGKLVSLSEQQLVDCDHECDPEEEGSCDSGCNGGLMNSAFEYTLKTGGLMREKDYPYTG 248

Query: 245 TDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGVSCPY 304
           TD G+CK D+SKI ASV+NFSVVS++E+QIAANL+KNGPLAVAINA +MQTYI GVSCPY
Sbjct: 249 TDGGSCKLDRSKIVASVSNFSVVSINEDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPY 308

Query: 305 ICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNICGVDS 364
           ICS+ L+HGVLLVGYGS G++  RLK+KPYWIIKNSWG +WGENG+YKIC+GRNICGVDS
Sbjct: 309 ICSRRLNHGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGENGFYKICKGRNICGVDS 358

Query: 365 MVSTVAA 372
           +VSTVAA
Sbjct: 369 LVSTVAA 358

BLAST of CSPI02G12590 vs. TAIR 10
Match: AT3G54940.2 (Papain family cysteine protease )

HSP 1 Score: 420.6 bits (1080), Expect = 1.3e-117
Identity = 203/347 (58.50%), Postives = 259/347 (74.64%), Query Frame = 0

Query: 31  DFIIRQVVDDGGVNEGSNGDDLLLGADHHFSVFKQKFGKSYASKEEHDHRFRVFKANLKR 90
           D  IRQV  D   N     + L    +  F +F   +GK+Y+++EE+ HR  +F  N+ +
Sbjct: 25  DLTIRQVTAD---NRRIRPNLLGTHTESKFRLFMSDYGKNYSTREEYIHRLGIFAKNVLK 84

Query: 91  AQRHQALDPSATHGVTQFSDLTPSEFRRSFLGL------RSRRLGLPADANKAPILPTDG 150
           A  HQ +DPSA HGVTQFSDLT  EF+R + G+      R   +G      +AP++  DG
Sbjct: 85  AAEHQMMDPSAVHGVTQFSDLTEEEFKRMYTGVADVGGSRGGTVGA-----EAPMVEVDG 144

Query: 151 LPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEGANFLATGKLVSLSEQQLVDCDHEC 210
           LP DFDWR+KG V+EVKNQG+CGSCW+FS TGA EGA+F++TGKL+SLSEQQLVDCD  C
Sbjct: 145 LPEDFDWREKGGVTEVKNQGACGSCWAFSTTGAAEGAHFVSTGKLLSLSEQQLVDCDQAC 204

Query: 211 DPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDYPYTGTDRGTCKFDKSKIAASVANF 270
           DP++K +CD+GC GGLM +A+EY +++GGL +E+ YPYTG  RG CKFD  K+A  V NF
Sbjct: 205 DPKDKKACDNGCGGGLMTNAYEYLMEAGGLEEERSYPYTG-KRGHCKFDPEKVAVRVLNF 264

Query: 271 SVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGVSCPYICSK-HLDHGVLLVGYGSDG 330
           + + LDE QIAANLV++GPLAV +NAVFMQTYI GVSCP ICSK +++HGVLLVGYGS G
Sbjct: 265 TTIPLDENQIAANLVRHGPLAVGLNAVFMQTYIGGVSCPLICSKRNVNHGVLLVGYGSKG 324

Query: 331 YAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNICGVDSMVSTVA 371
           ++ +RL +KPYWIIKNSWG  WGENGYYK+CRG +ICG++SMVS VA
Sbjct: 325 FSILRLSNKPYWIIKNSWGKKWGENGYYKLCRGHDICGINSMVSAVA 362

BLAST of CSPI02G12590 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 237.7 bits (605), Expect = 1.6e-62
Identity = 134/310 (43.23%), Postives = 183/310 (59.03%), Query Frame = 0

Query: 69  KSYASKEEHDHRFRVFKANLKRAQRHQALDPSATH--GVTQFSDLTPSEFRRSFLGLRSR 128
           K+Y    E + RF +FK NLK  + H ++ P+ T+  G+T+F+DLT  EFR  +L  +  
Sbjct: 52  KNYNGLGEKERRFEIFKDNLKFVEEHSSI-PNRTYEVGLTRFADLTNDEFRAIYLRSKME 111

Query: 129 RLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEGANFLAT 188
           R  +P    K      D LP   DWR KGAV+ VK+QGSCGSCW+FSA GA+EG N + T
Sbjct: 112 RTRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSCWAFSAIGAVEGINQIKT 171

Query: 189 GKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDYPYTGTD 248
           G+L+SLSEQ+LVDCD         S + GC GGLM+ AF++ +++GG+  E+DYPY  TD
Sbjct: 172 GELISLSEQELVDCD--------TSYNDGCGGGLMDYAFKFIIENGGIDTEEDYPYIATD 231

Query: 249 RGTCKFDKSKI-AASVANFSVVSLDEEQIAANLVKNGPLAVAINA--VFMQTYIKGVSCP 308
              C  DK      ++  +  V  ++E+     + N P++VAI A     Q Y  GV   
Sbjct: 232 VNVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQLYTSGVFTG 291

Query: 309 YICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNI---- 368
             C   LDHGV+ VGYGS+G        + YWI++NSWG+NWGE+GY+K+   RNI    
Sbjct: 292 -TCGTSLDHGVVAVGYGSEG-------GQDYWIVRNSWGSNWGESGYFKL--ERNIKESS 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P432962.2e-15774.27Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1[more]
Q9SUL16.5e-15471.39Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 S... [more]
P258046.7e-15171.04Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1[more]
P432951.1e-15070.84Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 S... [more]
Q107168.0e-14467.82Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LPD02.3e-218100.00Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_2G249900 PE=3 SV=1[more]
A0A5D3BWN51.9e-21296.55Cysteine proteinase RD19a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3CE631.9e-21296.55cysteine proteinase RD19a-like OS=Cucumis melo OX=3656 GN=LOC103499710 PE=3 SV=1[more]
A0A7R6B0V11.8e-20794.16Cysteine proteinase 2 OS=Citrullus lanatus OX=3654 PE=2 SV=1[more]
A0A6J1FW381.2e-19890.19cysteine protease RD19A-like OS=Cucurbita moschata OX=3662 GN=LOC111448673 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_004150061.14.7e-218100.00cysteine protease RD19A [Cucumis sativus] >KGN61836.1 hypothetical protein Csa_0... [more]
XP_008460996.13.9e-21296.55PREDICTED: cysteine proteinase RD19a-like [Cucumis melo] >KAA0045619.1 cysteine ... [more]
ARA15252.13.7e-20794.16cysteine proteinase 2 [Citrullus lanatus][more]
XP_038901827.13.9e-20492.31cysteine protease RD19A-like [Benincasa hispida][more]
XP_022944124.12.4e-19890.19cysteine protease RD19A-like [Cucurbita moschata] >KAG7010902.1 Cysteine proteas... [more]
Match NameE-valueIdentityDescription
AT4G39090.11.5e-15874.27Papain family cysteine protease [more]
AT4G16190.14.6e-15571.39Papain family cysteine protease [more]
AT2G21430.18.1e-15270.84Papain family cysteine protease [more]
AT3G54940.21.3e-11758.50Papain family cysteine protease [more]
AT3G19390.11.6e-6243.23Granulin repeat cysteine protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 163..178
score: 64.35
coord: 312..322
score: 60.13
coord: 334..340
score: 75.14
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 145..370
e-value: 4.1E-112
score: 388.4
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 145..368
e-value: 2.4E-75
score: 253.4
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 60..116
e-value: 6.9E-23
score: 92.0
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 60..116
e-value: 1.9E-12
score: 47.4
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 44..370
e-value: 3.2E-101
score: 341.3
NoneNo IPR availablePANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 38..371
NoneNo IPR availablePANTHERPTHR12411:SF783CYSTEINE PROTEASE RD19C-RELATEDcoord: 38..371
IPR025660Cysteine peptidase, histidine active sitePROSITEPS00639THIOL_PROTEASE_HIScoord: 310..320
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 163..174
IPR025661Cysteine peptidase, asparagine active sitePROSITEPS00640THIOL_PROTEASE_ASNcoord: 334..353
IPR039417Papain-like cysteine endopeptidaseCDDcd02248Peptidase_C1Acoord: 146..369
e-value: 4.12031E-104
score: 303.776
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 57..369

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G12590.1CSPI02G12590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0005615 extracellular space
cellular_component GO:0005764 lysosome
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0008234 cysteine-type peptidase activity