CSPI02G10300 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G10300
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description11S globulin subunit beta-like
LocationChr2: 10356777 .. 10358644 (-)
RNA-Seq ExpressionCSPI02G10300
SyntenyCSPI02G10300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGTGGATCTTCTTGCTCCACAACAACGCTCAGAAACGCCATGGCAACTGGTGCGTCTCTTCATCAATCCCTCCACCACGTATTCTCTTACCATCGCCGCCCTCCACTTGTGTCACCACCTATAAATTCTCCGCCACCTTCCTCATCACTCTAACCAACCCAACCACCCTTCTCTTCTCTTCTCCCTCACCATGCCTCACTATTCTCCTCTTGCTTTCTTATGTTTTGCAGTTTTCATCCATGGCTGCCTCTCCCAGACCGACCAATTTCCCTGGAGTTGGGGCTTCCAAGGCAGTGAAGCTAGGCAACAGCACCGTTTCCACTCTCCCAAACCCTGCCATCTCGAGAATCTCAGAGCTCAAGACCCCGTTCGCCGGATTGAGGCGGAGGGTGGTTTCACTGAATTATGGGAGCCCAACAGTGAGGAGCTCCAGTGTGCCGGAGTAAATATGGTCCGCCATACAATCCGACCCAGAGGTCTGCTTTTACCTGGATTCACTAATGCTCCTAAACTTGTTTTCGTCGTCCAAGGTACTGGTATTCGAGGTGTTGCAATGCCTGGCTGTCCTGAGACTTACCAAACCGATTTACGAAGATCACAATCGTCTGGAAACTTCAGAGACCAGCACCAGAAGATCCGCCAGTTCAGAGAGGGAGATCTCCTCGTCGTCCCTGCCGGAGTTTCTCACTGGATGTATAATCGTGGACAGTCTGATCTCATCTTGATTGTGTTCGTTGATACACGCAATGTCGCCAATCAAATTGATCCATTCGCCAGAGTAAGTTGGGGTAAAAAAAAAAAAAAGAACAACAACAACAGAGTGTAACTATTTAATTACTGATTTGAACAAGAACCACTAAGATTATTGGGTGTGTTTGTTACAGAAATTTTACCTCGCCGGAAGACCAGAGATGATGCGAAGAGGGGAATGGGAAGGAAGAAGCTATAGAGGATCTTCTGGAGAGAAATCAGGCAATGTGTTCAGTGGATTTGCAGATGAATTTTTAGAAGAAGCTTTCCAAGTAGATAGTGGATTGGTTAGGAAATTGAAGGGAGAAGACGACGAGAGGGATAGAATTGTGCTTGCCGAAGAAGATTTCGATGTGCTTATGCCGGAGAGAGACGATCAAGAAAGATCAAGAGGAAGATACGTGGAATCAGAAACAGAAAATGGCTTCGAAGAAACCGTATGCACACTCCGATTGAAGCATAGCATTGGCCGATCTGAACACGCCGATGTGTTCAACACACGTGGCGGTCGCATCTCCACCGCCAACTTCAATAACCTCCCCTTCCTCCGCCAAGTCCGCCTCAGCGCCGAACGAGGAGTCCTCTACAGCGTAAGTGAAGACTCCAAAATATTTTTGCTCAATTTGAAAACCCTAACAAAAACAGTAACTGAATGATCATATCGCCACTTTCAGAAATCCTAACAAGCCAAAAAATCTTATAACACCTCTAATTTTCTTCAAATTTTCCATTGCAGAAAGCGATATCTGCACCGCACTACACAGTGAACGCTCATACATTGGCGTACGCTACGAGAGGTAGCGCAAGAGTGCAAGTGGTGGACAACTACGGTCAGTCGGTGTTTGACGGGGAGGTCCGGGAAGGACAGGTAGTGATGATTCCACAGAACTTCGTGGTGATGACAAAAGCAGGCGATAGAGGATTCGAGTGGATTGCATTCAAGACAAACGACAACGCAATCACGAACTTGCTGGCGGGAAGAGTTTCGCAGATGAGATTGCTTCCGTTGGGAGTTGTGTCGAACATGTACAAAATTTCAAGGGAGGAGGCGCAGCGGCTGAAGTACGGGCAACCGGAGATGAGGATTTTCAGTCCAGGAAGGACGCAGAGATGA

mRNA sequence

ATGAGGTGGATCTTCTTGCTCCACAACAACGCTCAGAAACGCCATGGCAACTGGTGCGTCTCTTCATCAATCCCTCCACCACTTTTCATCCATGGCTGCCTCTCCCAGACCGACCAATTTCCCTGGAGTTGGGGCTTCCAAGGCAGTGAAGCTAGGCAACAGCACCGTTTCCACTCTCCCAAACCCTGCCATCTCGAGAATCTCAGAGCTCAAGACCCCGTTCGCCGGATTGAGGCGGAGGGTGGTTTCACTGAATTATGGGAGCCCAACAGTGAGGAGCTCCAGTGTGCCGGAGTAAATATGGTCCGCCATACAATCCGACCCAGAGGTCTGCTTTTACCTGGATTCACTAATGCTCCTAAACTTGTTTTCGTCGTCCAAGGTACTGGTATTCGAGGTGTTGCAATGCCTGGCTGTCCTGAGACTTACCAAACCGATTTACGAAGATCACAATCGTCTGGAAACTTCAGAGACCAGCACCAGAAGATCCGCCAGTTCAGAGAGGGAGATCTCCTCGTCGTCCCTGCCGGAGTTTCTCACTGGATGTATAATCGTGGACAGTCTGATCTCATCTTGATTGTGTTCGTTGATACACGCAATGTCGCCAATCAAATTGATCCATTCGCCAGAAAATTTTACCTCGCCGGAAGACCAGAGATGATGCGAAGAGGGGAATGGGAAGGAAGAAGCTATAGAGGATCTTCTGGAGAGAAATCAGGCAATGTGTTCAGTGGATTTGCAGATGAATTTTTAGAAGAAGCTTTCCAAGTAGATAGTGGATTGGTTAGGAAATTGAAGGGAGAAGACGACGAGAGGGATAGAATTGTGCTTGCCGAAGAAGATTTCGATGTGCTTATGCCGGAGAGAGACGATCAAGAAAGATCAAGAGGAAGATACGTGGAATCAGAAACAGAAAATGGCTTCGAAGAAACCGTATGCACACTCCGATTGAAGCATAGCATTGGCCGATCTGAACACGCCGATGTGTTCAACACACGTGGCGGTCGCATCTCCACCGCCAACTTCAATAACCTCCCCTTCCTCCGCCAAGTCCGCCTCAGCGCCGAACGAGGAGTCCTCTACAGCAAAGCGATATCTGCACCGCACTACACAGTGAACGCTCATACATTGGCGTACGCTACGAGAGGTAGCGCAAGAGTGCAAGTGGTGGACAACTACGGTCAGTCGGTGTTTGACGGGGAGGTCCGGGAAGGACAGGTAGTGATGATTCCACAGAACTTCGTGGTGATGACAAAAGCAGGCGATAGAGGATTCGAGTGGATTGCATTCAAGACAAACGACAACGCAATCACGAACTTGCTGGCGGGAAGAGTTTCGCAGATGAGATTGCTTCCGTTGGGAGTTGTGTCGAACATGTACAAAATTTCAAGGGAGGAGGCGCAGCGGCTGAAGTACGGGCAACCGGAGATGAGGATTTTCAGTCCAGGAAGGACGCAGAGATGA

Coding sequence (CDS)

ATGAGGTGGATCTTCTTGCTCCACAACAACGCTCAGAAACGCCATGGCAACTGGTGCGTCTCTTCATCAATCCCTCCACCACTTTTCATCCATGGCTGCCTCTCCCAGACCGACCAATTTCCCTGGAGTTGGGGCTTCCAAGGCAGTGAAGCTAGGCAACAGCACCGTTTCCACTCTCCCAAACCCTGCCATCTCGAGAATCTCAGAGCTCAAGACCCCGTTCGCCGGATTGAGGCGGAGGGTGGTTTCACTGAATTATGGGAGCCCAACAGTGAGGAGCTCCAGTGTGCCGGAGTAAATATGGTCCGCCATACAATCCGACCCAGAGGTCTGCTTTTACCTGGATTCACTAATGCTCCTAAACTTGTTTTCGTCGTCCAAGGTACTGGTATTCGAGGTGTTGCAATGCCTGGCTGTCCTGAGACTTACCAAACCGATTTACGAAGATCACAATCGTCTGGAAACTTCAGAGACCAGCACCAGAAGATCCGCCAGTTCAGAGAGGGAGATCTCCTCGTCGTCCCTGCCGGAGTTTCTCACTGGATGTATAATCGTGGACAGTCTGATCTCATCTTGATTGTGTTCGTTGATACACGCAATGTCGCCAATCAAATTGATCCATTCGCCAGAAAATTTTACCTCGCCGGAAGACCAGAGATGATGCGAAGAGGGGAATGGGAAGGAAGAAGCTATAGAGGATCTTCTGGAGAGAAATCAGGCAATGTGTTCAGTGGATTTGCAGATGAATTTTTAGAAGAAGCTTTCCAAGTAGATAGTGGATTGGTTAGGAAATTGAAGGGAGAAGACGACGAGAGGGATAGAATTGTGCTTGCCGAAGAAGATTTCGATGTGCTTATGCCGGAGAGAGACGATCAAGAAAGATCAAGAGGAAGATACGTGGAATCAGAAACAGAAAATGGCTTCGAAGAAACCGTATGCACACTCCGATTGAAGCATAGCATTGGCCGATCTGAACACGCCGATGTGTTCAACACACGTGGCGGTCGCATCTCCACCGCCAACTTCAATAACCTCCCCTTCCTCCGCCAAGTCCGCCTCAGCGCCGAACGAGGAGTCCTCTACAGCAAAGCGATATCTGCACCGCACTACACAGTGAACGCTCATACATTGGCGTACGCTACGAGAGGTAGCGCAAGAGTGCAAGTGGTGGACAACTACGGTCAGTCGGTGTTTGACGGGGAGGTCCGGGAAGGACAGGTAGTGATGATTCCACAGAACTTCGTGGTGATGACAAAAGCAGGCGATAGAGGATTCGAGTGGATTGCATTCAAGACAAACGACAACGCAATCACGAACTTGCTGGCGGGAAGAGTTTCGCAGATGAGATTGCTTCCGTTGGGAGTTGTGTCGAACATGTACAAAATTTCAAGGGAGGAGGCGCAGCGGCTGAAGTACGGGCAACCGGAGATGAGGATTTTCAGTCCAGGAAGGACGCAGAGATGA

Protein sequence

MRWIFLLHNNAQKRHGNWCVSSSIPPPLFIHGCLSQTDQFPWSWGFQGSEARQQHRFHSPKPCHLENLRAQDPVRRIEAEGGFTELWEPNSEELQCAGVNMVRHTIRPRGLLLPGFTNAPKLVFVVQGTGIRGVAMPGCPETYQTDLRRSQSSGNFRDQHQKIRQFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQIDPFARKFYLAGRPEMMRRGEWEGRSYRGSSGEKSGNVFSGFADEFLEEAFQVDSGLVRKLKGEDDERDRIVLAEEDFDVLMPERDDQERSRGRYVESETENGFEETVCTLRLKHSIGRSEHADVFNTRGGRISTANFNNLPFLRQVRLSAERGVLYSKAISAPHYTVNAHTLAYATRGSARVQVVDNYGQSVFDGEVREGQVVMIPQNFVVMTKAGDRGFEWIAFKTNDNAITNLLAGRVSQMRLLPLGVVSNMYKISREEAQRLKYGQPEMRIFSPGRTQR*
Homology
BLAST of CSPI02G10300 vs. ExPASy Swiss-Prot
Match: P13744 (11S globulin subunit beta OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 7.2e-214
Identity = 364/464 (78.45%), Postives = 415/464 (89.44%), Query Frame = 0

Query: 28  LFIHGCLSQTDQFPWSWGFQGSEARQQHRFHSPKPCHLENLRAQDPVRRIEAEGGFTELW 87
           +FI+GCLSQ +Q    W FQGSE  QQHR+ SP+ C LENLRAQDPVRR EAE  FTE+W
Sbjct: 14  VFINGCLSQIEQ-QSPWEFQGSEVWQQHRYQSPRACRLENLRAQDPVRRAEAEAIFTEVW 73

Query: 88  EPNSEELQCAGVNMVRHTIRPRGLLLPGFTNAPKLVFVVQGTGIRGVAMPGCPETYQTDL 147
           + +++E QCAGVNM+RHTIRP+GLLLPGF+NAPKL+FV QG GIRG+A+PGC ETYQTDL
Sbjct: 74  DQDNDEFQCAGVNMIRHTIRPKGLLLPGFSNAPKLIFVAQGFGIRGIAIPGCAETYQTDL 133

Query: 148 RRSQSSGN-FRDQHQKIRQFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQID 207
           RRSQS+G+ F+DQHQKIR FREGDLLVVPAGVSHWMYNRGQSDL+LIVF DTRNVANQID
Sbjct: 134 RRSQSAGSAFKDQHQKIRPFREGDLLVVPAGVSHWMYNRGQSDLVLIVFADTRNVANQID 193

Query: 208 PFARKFYLAGRPEMMRRG--EWEGRSYRGSSGEKSGNVFSGFADEFLEEAFQVDSGLVRK 267
           P+ RKFYLAGRPE + RG  EWE  S +GSSGEKSGN+FSGFADEFLEEAFQ+D GLVRK
Sbjct: 194 PYLRKFYLAGRPEQVERGVEEWERSSRKGSSGEKSGNIFSGFADEFLEEAFQIDGGLVRK 253

Query: 268 LKGEDDERDRIVLAEEDFDVLMPERDDQERSRGRYV--ESETENGFEETVCTLRLKHSIG 327
           LKGEDDERDRIV  +EDF+VL+PE+D++ERSRGRY+  ESE+ENG EET+CTLRLK +IG
Sbjct: 254 LKGEDDERDRIVQVDEDFEVLLPEKDEEERSRGRYIESESESENGLEETICTLRLKQNIG 313

Query: 328 RSEHADVFNTRGGRISTANFNNLPFLRQVRLSAERGVLYSKAISAPHYTVNAHTLAYATR 387
           RS  ADVFN RGGRISTAN++ LP LRQVRLSAERGVLYS A+ APHYTVN+H++ YATR
Sbjct: 314 RSVRADVFNPRGGRISTANYHTLPILRQVRLSAERGVLYSNAMVAPHYTVNSHSVMYATR 373

Query: 388 GSARVQVVDNYGQSVFDGEVREGQVVMIPQNFVVMTKAGDRGFEWIAFKTNDNAITNLLA 447
           G+ARVQVVDN+GQSVFDGEVREGQV+MIPQNFVV+ +A DRGFEWIAFKTNDNAITNLLA
Sbjct: 374 GNARVQVVDNFGQSVFDGEVREGQVLMIPQNFVVIKRASDRGFEWIAFKTNDNAITNLLA 433

Query: 448 GRVSQMRLLPLGVVSNMYKISREEAQRLKYGQPEMRIFSPGRTQ 487
           GRVSQMR+LPLGV+SNMY+ISREEAQRLKYGQ EMR+ SPGR+Q
Sbjct: 434 GRVSQMRMLPLGVLSNMYRISREEAQRLKYGQQEMRVLSPGRSQ 476

BLAST of CSPI02G10300 vs. ExPASy Swiss-Prot
Match: B5KVH4 (11S globulin seed storage protein 1 OS=Carya illinoinensis OX=32201 PE=1 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 8.2e-117
Identity = 229/482 (47.51%), Postives = 303/482 (62.86%), Query Frame = 0

Query: 31  HGCLSQTDQFPWSWGFQGSEARQQHRFHSPKPCHLENLRAQDPVRRIEAEGGFTELWEPN 90
           +GCL+Q            S  RQQH+F     C L  L A +P  RIEAE G  E W+PN
Sbjct: 20  NGCLAQ------------SGGRQQHKFGQ---CQLNRLDALEPTNRIEAEAGVIESWDPN 79

Query: 91  SEELQCAGVNMVRHTIRPRGLLLPGFTNAPKLVFVVQGTGIRGVAMPGCPETYQTDLRRS 150
            ++LQCAGV +VR TI P GLLLP ++NAP+LV++ +G GI GV  PGCPET++   R+S
Sbjct: 80  HQQLQCAGVAVVRRTIEPNGLLLPHYSNAPQLVYIARGRGITGVLFPGCPETFEESQRQS 139

Query: 151 QSSGN---FRDQHQKIRQFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQIDP 210
           Q        +D+HQKIR FREGD++  PAGV+HW YN G S ++ I  +DT N ANQ+D 
Sbjct: 140 QQGQRREFQQDRHQKIRHFREGDIIAFPAGVAHWCYNDGSSPVVAIFLLDTHNNANQLDQ 199

Query: 211 FARKFYLAGRPE-----------MMRRGEWEGRSYRGSSGEK----SGNVFSGFADEFLE 270
             R FYLAG P+              R + + +  RG  GE+      NVFSGF  EFL 
Sbjct: 200 NPRNFYLAGNPDDEFRPQGQQEYEQHRRQQQHQQRRGEHGEQQRDLGNNVFSGFDAEFLA 259

Query: 271 EAFQVDSGLVRKLKGEDDERDRIVLAE-EDFDVLMP--ERDDQE----RSRGRYVESETE 330
           +AF VD+   R+L+ E+D R  IV  E     V+ P   R++QE    + R R  ESE+E
Sbjct: 260 DAFNVDTETARRLQSENDHRGSIVRVEGRQLQVIRPRWSREEQEHEERKERERERESESE 319

Query: 331 -----------NGFEETVCTLRLKHSIGRSEHADVFNTRGGRISTANFNNLPFLRQVRLS 390
                      NG EET+CTL L+ +IG    AD++    GRIST N +NLP LR ++LS
Sbjct: 320 RRQSRRGGRDDNGLEETICTLSLRENIGDPSRADIYTEEAGRISTVNSHNLPILRWLQLS 379

Query: 391 AERGVLYSKAISAPHYTVNAHTLAYATRGSARVQVVDNYGQSVFDGEVREGQVVMIPQNF 450
           AERG LYS A+  PH+ +NAH++ YA RG A VQVVDN+GQ+VFD E+REGQ++ IPQNF
Sbjct: 380 AERGALYSDALYVPHWNLNAHSVVYALRGRAEVQVVDNFGQTVFDDELREGQLLTIPQNF 439

Query: 451 VVMTKAGDRGFEWIAFKTNDNAITNLLAGRVSQMRLLPLGVVSNMYKISREEAQRLKYGQ 477
            V+ +A D GFEW++FKTN+NA+ + LAGR S +R LP  V+ N ++I RE+A+RLK+ +
Sbjct: 440 AVVKRARDEGFEWVSFKTNENAMVSPLAGRTSAIRALPEEVLVNAFQIPREDARRLKFNR 486

BLAST of CSPI02G10300 vs. ExPASy Swiss-Prot
Match: A0A1L6K371 (11S globulin OS=Juglans nigra OX=16719 PE=1 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 1.0e-114
Identity = 230/487 (47.23%), Postives = 310/487 (63.66%), Query Frame = 0

Query: 30  IHGCLSQTDQFPWSWGFQGSEARQQHRFHSPKPCHLENLRAQDPVRRIEAEGGFTELWEP 89
           ++GCL+Q            S  RQQ RF     C L+ L A +P  RIEAE G  E W+P
Sbjct: 17  VNGCLAQ------------SGGRQQPRFGE---CKLKRLVALEPSNRIEAEAGVIESWDP 76

Query: 90  NSEELQCAGVNMVRHTIRPRGLLLPGFTNAPKLVFVVQGTGIRGVAMPGCPETY------ 149
           N+++ QCAGV +VR TI P GLLLP ++NAP+L+++V+G GI GV  PGCPET+      
Sbjct: 77  NNQQFQCAGVAVVRRTIEPNGLLLPQYSNAPQLLYIVKGRGITGVLFPGCPETFEESQQG 136

Query: 150 QTDLRRSQSSGNF-RDQHQKIRQFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVA 209
           Q+ +R S  S +F RD+HQKIR FREGD++  PAGV+HW YN G + ++ +  +DT N A
Sbjct: 137 QSRIRPSLRSASFQRDRHQKIRHFREGDVIAFPAGVAHWCYNDGDTPVVAVALMDTTNNA 196

Query: 210 NQIDPFARKFYLAGRP--EMMRRGEWEGRSYR---------GSSGEK----SGNVFSGFA 269
           NQ+D   R FYLAG P  E   +G+ E   +R         G  G++      NVFSGF 
Sbjct: 197 NQLDQNPRNFYLAGNPDDEFRPQGQQEYEQHRRQQQHQQRHGEPGQQQRGSGNNVFSGFD 256

Query: 270 DEFLEEAFQVDSGLVRKLKGEDDERDRIVLAE-EDFDVLMP--ERDDQER----SRGRYV 329
            +FL +AF VD+   R+L+ E+D R  IV  E     V+ P   R++QER     R R  
Sbjct: 257 ADFLADAFNVDTETARRLQSENDHRRSIVRVEGRQLQVIRPRWSREEQEREERKERERER 316

Query: 330 ESETE-----------NGFEETVCTLRLKHSIGRSEHADVFNTRGGRISTANFNNLPFLR 389
           ESE+E           NG EET+CTLRL+ +IG    AD++    GRISTAN + LP LR
Sbjct: 317 ESESERRQSRRGGRDDNGLEETICTLRLRENIGDPSRADIYTEEAGRISTANSHTLPVLR 376

Query: 390 QVRLSAERGVLYSKAISAPHYTVNAHTLAYATRGSARVQVVDNYGQSVFDGEVREGQVVM 449
            ++LSAERG LYS A+  PH+ +NAH++ YA RG A VQVVDN+GQ+VFD E+REGQ++ 
Sbjct: 377 WLQLSAERGALYSDALYVPHWNLNAHSVVYALRGRAEVQVVDNFGQTVFDDELREGQLLT 436

Query: 450 IPQNFVVMTKAGDRGFEWIAFKTNDNAITNLLAGRVSQMRLLPLGVVSNMYKISREEAQR 477
           IPQNF V+ +A + GFEW++FKTN+NA+ + LAGR S +R LP  V++N  +I RE+A+R
Sbjct: 437 IPQNFAVVKRARNEGFEWVSFKTNENAMVSPLAGRTSAIRALPEEVLANALQIPREDARR 488

BLAST of CSPI02G10300 vs. ExPASy Swiss-Prot
Match: P09800 (Legumin B OS=Gossypium hirsutum OX=3635 GN=LEGB PE=2 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 6.5e-114
Identity = 230/513 (44.83%), Postives = 309/513 (60.23%), Query Frame = 0

Query: 20  VSSSIPPPLFIHGCLSQTD------QFPWSWGFQGSEARQQHRFHSPKPCHLENLRAQDP 79
           +S S+   +  HGC +Q D      Q P  WG Q  + + +H+      C L+NL A  P
Sbjct: 7   LSFSVCLLVLFHGCCAQIDLVTNHHQDP-PWG-QPQQPQPRHQ----SQCQLQNLNALQP 66

Query: 80  VRRIEAEGGFTELWEPNSEELQCAGVNMVRHTIRPRGLLLPGFTNAPKLVFVVQGTGIRG 139
             R  +E G TE W+ N ++ QCAGV  +RH I+ +GLLLP FT+AP L +V QG GI G
Sbjct: 67  KHRFRSEAGETEFWDQNEDQFQCAGVAFLRHKIQRKGLLLPSFTSAPMLFYVEQGEGIHG 126

Query: 140 VAMPGCPETYQTDLRRSQSSGNFRDQHQKIRQFREGDLLVVPAGVSHWMYNRGQSDLILI 199
              PGCPETYQ+  +++      RDQHQK+R+ +EGD++ +PAGV+HW++N G+S L+L+
Sbjct: 127 AVFPGCPETYQSQSQQNIQDRPQRDQHQKLRRLKEGDVVALPAGVAHWIFNNGRSQLVLV 186

Query: 200 VFVDTRNVANQIDPFARKFYLAGRPE--MMRRGEWEGRSYRGS-----------SGEKSG 259
             VD  N ANQ+D   RKF+LAG P+  ++R G+   R+ R S           S E  G
Sbjct: 187 ALVDVGNDANQLDENFRKFFLAGSPQGGVVRGGQSRDRNQRQSRTQRGEREEEESQESGG 246

Query: 260 -NVFSGFADEFLEEAFQVDSGLVRKLKGEDDERDRIVLAEEDFDVLMPERDDQERSRGRY 319
            NV SGF D  L +AF +D+ L RKL+ E D R  IV  E  F+   PE   + + R   
Sbjct: 247 NNVLSGFRDNLLAQAFGIDTRLARKLQNERDNRGAIVRMEHGFE--WPEEGQRRQGREEE 306

Query: 320 VESETE------------------------------NGFEETVCTLRLKHSIGRSEHADV 379
            E E E                              NG EET C++RLKH    S  ADV
Sbjct: 307 GEEEREPKWQRRQESQEEGSEEEEREERGRGRRRSGNGLEETFCSMRLKHRTPASS-ADV 366

Query: 380 FNTRGGRISTANFNNLPFLRQVRLSAERGVLYSKAISAPHYTVNAHTLAYATRGSARVQV 439
           FN RGGRI+T N  NLP L+ ++LSAERGVLY+ AI APH+ +NAH++ Y TRG+ R+Q+
Sbjct: 367 FNPRGGRITTVNSFNLPILQYLQLSAERGVLYNNAIYAPHWNMNAHSIVYITRGNGRIQI 426

Query: 440 VDNYGQSVFDGEVREGQVVMIPQNFVVMTKAGDRGFEWIAFKTNDNAITNLLAGRVSQMR 483
           V   G+++FD +V  GQV+ +PQN  V+ KAG RGFEWIAFKTN NA  + +AGRVS MR
Sbjct: 427 VSENGEAIFDEQVERGQVITVPQNHAVVKKAGRRGFEWIAFKTNANAKISQIAGRVSIMR 486

BLAST of CSPI02G10300 vs. ExPASy Swiss-Prot
Match: Q2TPW5 (11S globulin seed storage protein Jug r 4 OS=Juglans regia OX=51240 PE=1 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 8.5e-114
Identity = 227/482 (47.10%), Postives = 305/482 (63.28%), Query Frame = 0

Query: 31  HGCLSQTDQFPWSWGFQGSEARQQHRFHSPKPCHLENLRAQDPVRRIEAEGGFTELWEPN 90
           +GCL+Q            S  RQQ +F     C L  L A +P  RIEAE G  E W+PN
Sbjct: 19  NGCLAQ------------SGGRQQQQFGQ---CQLNRLDALEPTNRIEAEAGVIESWDPN 78

Query: 91  SEELQCAGVNMVRHTIRPRGLLLPGFTNAPKLVFVVQGTGIRGVAMPGCPETYQTDLRRS 150
           +++ QCAGV +VR TI P GLLLP ++NAP+LV++ +G GI GV  PGCPET++   R+S
Sbjct: 79  NQQFQCAGVAVVRRTIEPNGLLLPQYSNAPQLVYIARGRGITGVLFPGCPETFEESQRQS 138

Query: 151 Q--SSGNF-RDQHQKIRQFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQIDP 210
           Q   S  F +D+HQKIR FREGD++  PAGV+HW YN G + ++ I  +DT N ANQ+D 
Sbjct: 139 QQGQSREFQQDRHQKIRHFREGDIIAFPAGVAHWSYNDGSNPVVAISLLDTNNNANQLDQ 198

Query: 211 FARKFYLAGRP--EMMRRGEWEGRSYR---------GSSGEK----SGNVFSGFADEFLE 270
             R FYLAG P  E   +G+ E   +R         G  G++      NVFSGF  +FL 
Sbjct: 199 NPRNFYLAGNPDDEFRPQGQQEYEQHRRQQQRQQRPGEHGQQQRGLGNNVFSGFDADFLA 258

Query: 271 EAFQVDSGLVRKLKGEDDERDRIVLAE-EDFDVLMP--ERDDQER----SRGRYVESETE 330
           +AF VD+   R+L+ E+D R  IV  E     V+ P   R++QER     R R  ESE+E
Sbjct: 259 DAFNVDTETARRLQSENDHRRSIVRVEGRQLQVIRPRWSREEQEREERKERERERESESE 318

Query: 331 -----------NGFEETVCTLRLKHSIGRSEHADVFNTRGGRISTANFNNLPFLRQVRLS 390
                      NG EET+CTLRL+ +IG    AD++    GRIST N + LP LR ++LS
Sbjct: 319 RRQSRRGGRDDNGLEETICTLRLRENIGDPSRADIYTEEAGRISTVNSHTLPVLRWLQLS 378

Query: 391 AERGVLYSKAISAPHYTVNAHTLAYATRGSARVQVVDNYGQSVFDGEVREGQVVMIPQNF 450
           AERG LYS A+  PH+ +NAH++ YA RG A VQVVDN+GQ+VFD E+REGQ++ IPQNF
Sbjct: 379 AERGALYSDALYVPHWNLNAHSVVYALRGRAEVQVVDNFGQTVFDDELREGQLLTIPQNF 438

Query: 451 VVMTKAGDRGFEWIAFKTNDNAITNLLAGRVSQMRLLPLGVVSNMYKISREEAQRLKYGQ 477
            V+ +A + GFEW++FKTN+NA+ + LAGR S +R LP  V++  ++I RE+A+RLK+ +
Sbjct: 439 AVVKRARNEGFEWVSFKTNENAMVSPLAGRTSAIRALPEEVLATAFQIPREDARRLKFNR 485

BLAST of CSPI02G10300 vs. ExPASy TrEMBL
Match: A0A0A0LNN6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G174130 PE=3 SV=1)

HSP 1 Score: 994.6 bits (2570), Expect = 1.5e-286
Identity = 485/487 (99.59%), Postives = 486/487 (99.79%), Query Frame = 0

Query: 1   MRWIFLLHNNAQKRHGNWCVSSSIPPPLFIHGCLSQTDQFPWSWGFQGSEARQQHRFHSP 60
           MRWIFLLHNNAQKRHGNWCVSSSIPPPLFIHGCLSQTDQFPWSWGFQGSEARQQHRFHSP
Sbjct: 1   MRWIFLLHNNAQKRHGNWCVSSSIPPPLFIHGCLSQTDQFPWSWGFQGSEARQQHRFHSP 60

Query: 61  KPCHLENLRAQDPVRRIEAEGGFTELWEPNSEELQCAGVNMVRHTIRPRGLLLPGFTNAP 120
           KPCHLENLRAQDPVRRIEAEGGFTELWEPNSEELQCAGVNMVRHTIRPRGLLLPGFTNAP
Sbjct: 61  KPCHLENLRAQDPVRRIEAEGGFTELWEPNSEELQCAGVNMVRHTIRPRGLLLPGFTNAP 120

Query: 121 KLVFVVQGTGIRGVAMPGCPETYQTDLRRSQSSGNFRDQHQKIRQFREGDLLVVPAGVSH 180
           KLVFVVQGTGIRGVAMPGCPETYQTDLRRSQSSGNFRDQHQKIRQFREGDLLVVPAGVSH
Sbjct: 121 KLVFVVQGTGIRGVAMPGCPETYQTDLRRSQSSGNFRDQHQKIRQFREGDLLVVPAGVSH 180

Query: 181 WMYNRGQSDLILIVFVDTRNVANQIDPFARKFYLAGRPEMMRRGEWEGRSYRGSSGEKSG 240
           WMYNRGQSDLILIVFVDTRNVANQIDPFARKFYLAGRPEMMRRGEWEGRSYRGSSGEKSG
Sbjct: 181 WMYNRGQSDLILIVFVDTRNVANQIDPFARKFYLAGRPEMMRRGEWEGRSYRGSSGEKSG 240

Query: 241 NVFSGFADEFLEEAFQVDSGLVRKLKGEDDERDRIVLAEEDFDVLMPERDDQERSRGRYV 300
           NV SGFADEFLEEAFQVDSGLVRKLKGEDDERDRIVLAEEDFDVLMPERDDQERSRGRYV
Sbjct: 241 NVVSGFADEFLEEAFQVDSGLVRKLKGEDDERDRIVLAEEDFDVLMPERDDQERSRGRYV 300

Query: 301 ESETENGFEETVCTLRLKHSIGRSEHADVFNTRGGRISTANFNNLPFLRQVRLSAERGVL 360
           ESETENGFEETVCTLRLKHSIGRSEHADVFNTRGGRISTANFNNLPFLRQVRLSAERGVL
Sbjct: 301 ESETENGFEETVCTLRLKHSIGRSEHADVFNTRGGRISTANFNNLPFLRQVRLSAERGVL 360

Query: 361 YSKAISAPHYTVNAHTLAYATRGSARVQVVDNYGQSVFDGEVREGQVVMIPQNFVVMTKA 420
           Y+KAISAPHYTVNAHTLAYATRGSARVQVVDNYGQSVFDGEVREGQVVMIPQNFVVMTKA
Sbjct: 361 YNKAISAPHYTVNAHTLAYATRGSARVQVVDNYGQSVFDGEVREGQVVMIPQNFVVMTKA 420

Query: 421 GDRGFEWIAFKTNDNAITNLLAGRVSQMRLLPLGVVSNMYKISREEAQRLKYGQPEMRIF 480
           GDRGFEWIAFKTNDNAITNLLAGRVSQMRLLPLGVVSNMYKISREEAQRLKYGQPEMRIF
Sbjct: 421 GDRGFEWIAFKTNDNAITNLLAGRVSQMRLLPLGVVSNMYKISREEAQRLKYGQPEMRIF 480

Query: 481 SPGRTQR 488
           SPGRTQR
Sbjct: 481 SPGRTQR 487

BLAST of CSPI02G10300 vs. ExPASy TrEMBL
Match: A0A0A0K9P5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G452090 PE=3 SV=1)

HSP 1 Score: 867.8 bits (2241), Expect = 2.1e-248
Identity = 417/459 (90.85%), Postives = 446/459 (97.17%), Query Frame = 0

Query: 28  LFIHGCLSQTDQFPWSWGFQGSEARQQHRFHSPKPCHLENLRAQDPVRRIEAEGGFTELW 87
           +FI+GCLSQTDQFPWSWGFQGSEARQQHRFHSPK CHLENLRA++P RRIEAE G+TELW
Sbjct: 14  VFINGCLSQTDQFPWSWGFQGSEARQQHRFHSPKACHLENLRAEEPARRIEAEAGYTELW 73

Query: 88  EPNSEELQCAGVNMVRHTIRPRGLLLPGFTNAPKLVFVVQGTGIRGVAMPGCPETYQTDL 147
           EPN+EE QCAGVNMVRHTIRP+GLLLPGFTNAPKL+FVVQGTGIRGVAMPGCPETY+TDL
Sbjct: 74  EPNNEEFQCAGVNMVRHTIRPKGLLLPGFTNAPKLIFVVQGTGIRGVAMPGCPETYETDL 133

Query: 148 RRSQSSGNFRDQHQKIRQFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQIDP 207
           RRSQSSGNFRDQHQKIR+FREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQIDP
Sbjct: 134 RRSQSSGNFRDQHQKIREFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQIDP 193

Query: 208 FARKFYLAGRPEMMRRGEWEGRSYRGSSGEKSGNVFSGFADEFLEEAFQVDSGLVRKLKG 267
           FARKFYL+GRPEMMRRGE EGRS+R S+GEKSGNVFSGFADEFLEEA Q+DSGLVR+LKG
Sbjct: 194 FARKFYLSGRPEMMRRGEREGRSFRESTGEKSGNVFSGFADEFLEEALQIDSGLVRRLKG 253

Query: 268 EDDERDRIVLAEEDFDVLMPERDDQERSRGRYVESETENGFEETVCTLRLKHSIGRSEHA 327
           E DERDRIV AEEDFDVLMPE+DDQERSRGRY+E E+ENGFEETVCTLRLKH+IGRSEHA
Sbjct: 254 ESDERDRIVFAEEDFDVLMPEKDDQERSRGRYIEKESENGFEETVCTLRLKHNIGRSEHA 313

Query: 328 DVFNTRGGRISTANFNNLPFLRQVRLSAERGVLYSKAISAPHYTVNAHTLAYATRGSARV 387
           DVFN RGGR+STANFNNLPFLRQVRLSAERGVLYS AISAPHYTVNAHT+AYATRGSARV
Sbjct: 314 DVFNPRGGRLSTANFNNLPFLRQVRLSAERGVLYSNAISAPHYTVNAHTVAYATRGSARV 373

Query: 388 QVVDNYGQSVFDGEVREGQVVMIPQNFVVMTKAGDRGFEWIAFKTNDNAITNLLAGRVSQ 447
           QVVDNYGQ+VFDGEVREGQV++IPQNFVVMT+A +RGFEWIAFKTNDNAITNLLAGRVSQ
Sbjct: 374 QVVDNYGQAVFDGEVREGQVLVIPQNFVVMTRASERGFEWIAFKTNDNAITNLLAGRVSQ 433

Query: 448 MRLLPLGVVSNMYKISREEAQRLKYGQPEMRIFSPGRTQ 487
           MRLLPLGV+SNMY+ISREEAQRLKYGQPEMRIFSPGR+Q
Sbjct: 434 MRLLPLGVLSNMYRISREEAQRLKYGQPEMRIFSPGRSQ 472

BLAST of CSPI02G10300 vs. ExPASy TrEMBL
Match: A0A1S3CIE1 (11S globulin subunit beta-like OS=Cucumis melo OX=3656 GN=LOC103500763 PE=3 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 1.0e-247
Identity = 418/459 (91.07%), Postives = 444/459 (96.73%), Query Frame = 0

Query: 28  LFIHGCLSQTDQFPWSWGFQGSEARQQHRFHSPKPCHLENLRAQDPVRRIEAEGGFTELW 87
           +FI+GCLSQTDQFPWSWGFQGSEARQQHRFHSPK CHLENLRA++P RRIEAE G+TELW
Sbjct: 14  VFINGCLSQTDQFPWSWGFQGSEARQQHRFHSPKACHLENLRAEEPARRIEAEAGYTELW 73

Query: 88  EPNSEELQCAGVNMVRHTIRPRGLLLPGFTNAPKLVFVVQGTGIRGVAMPGCPETYQTDL 147
           EPN+EE QCAGVNMVRHTIRP+GLLLPGFTNAPKL+FVVQG GIRGVAMPGCPETY+TDL
Sbjct: 74  EPNNEEFQCAGVNMVRHTIRPKGLLLPGFTNAPKLIFVVQGAGIRGVAMPGCPETYETDL 133

Query: 148 RRSQSSGNFRDQHQKIRQFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQIDP 207
           RRSQSSGNFRDQHQKIR FREGDLLV+PAGVS W+YNRGQSDLIL+VFVDTRNVANQIDP
Sbjct: 134 RRSQSSGNFRDQHQKIRHFREGDLLVLPAGVSQWIYNRGQSDLILVVFVDTRNVANQIDP 193

Query: 208 FARKFYLAGRPEMMRRGEWEGRSYRGSSGEKSGNVFSGFADEFLEEAFQVDSGLVRKLKG 267
           FARKFYLAGRPEMMRRGE EGRS+R S+GEKSGNVFSGFADEFLEEAFQVDSGLVR+LKG
Sbjct: 194 FARKFYLAGRPEMMRRGEREGRSFRESTGEKSGNVFSGFADEFLEEAFQVDSGLVRRLKG 253

Query: 268 EDDERDRIVLAEEDFDVLMPERDDQERSRGRYVESETENGFEETVCTLRLKHSIGRSEHA 327
           E+D+RDRIV  EEDFDVLMPERDDQERSRGRYVE E+ENGFEETVCTLRLKH+IGRSEHA
Sbjct: 254 ENDDRDRIVFTEEDFDVLMPERDDQERSRGRYVEKESENGFEETVCTLRLKHNIGRSEHA 313

Query: 328 DVFNTRGGRISTANFNNLPFLRQVRLSAERGVLYSKAISAPHYTVNAHTLAYATRGSARV 387
           DVFN RGGRISTANFNNLPFLRQVRLSAERGVLYS AISAPHYTVNAHT+AYATRGSARV
Sbjct: 314 DVFNPRGGRISTANFNNLPFLRQVRLSAERGVLYSNAISAPHYTVNAHTVAYATRGSARV 373

Query: 388 QVVDNYGQSVFDGEVREGQVVMIPQNFVVMTKAGDRGFEWIAFKTNDNAITNLLAGRVSQ 447
           QVVDNYGQSVFDGEVREGQV++IPQNFVVMT+A DRGFEWIAFKTNDNAITNLLAGRVSQ
Sbjct: 374 QVVDNYGQSVFDGEVREGQVLVIPQNFVVMTRASDRGFEWIAFKTNDNAITNLLAGRVSQ 433

Query: 448 MRLLPLGVVSNMYKISREEAQRLKYGQPEMRIFSPGRTQ 487
           MRLLPLGV+SNMY+ISREEAQRLKYGQPEMRIFSPGR+Q
Sbjct: 434 MRLLPLGVLSNMYRISREEAQRLKYGQPEMRIFSPGRSQ 472

BLAST of CSPI02G10300 vs. ExPASy TrEMBL
Match: A0A5D3C0J2 (11S globulin subunit beta-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G00630 PE=3 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 3.0e-247
Identity = 417/459 (90.85%), Postives = 443/459 (96.51%), Query Frame = 0

Query: 28  LFIHGCLSQTDQFPWSWGFQGSEARQQHRFHSPKPCHLENLRAQDPVRRIEAEGGFTELW 87
           +FI+GCLSQTDQFPWSWGFQGSEARQQHRFHSPK CHLENLRA++P RRIEAE G+TELW
Sbjct: 22  VFINGCLSQTDQFPWSWGFQGSEARQQHRFHSPKACHLENLRAEEPARRIEAEAGYTELW 81

Query: 88  EPNSEELQCAGVNMVRHTIRPRGLLLPGFTNAPKLVFVVQGTGIRGVAMPGCPETYQTDL 147
           EPN+EE QCAGVNMVRHTIRP+GLLLPGFTNAPKL+FVVQG GIRGVAMPGCPETY+TDL
Sbjct: 82  EPNNEEFQCAGVNMVRHTIRPKGLLLPGFTNAPKLIFVVQGAGIRGVAMPGCPETYETDL 141

Query: 148 RRSQSSGNFRDQHQKIRQFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQIDP 207
           RRSQSSGNFRDQHQKIR FREGDLLV+PAGVS W+YNRGQSDLIL+VFVDTRNVANQIDP
Sbjct: 142 RRSQSSGNFRDQHQKIRHFREGDLLVLPAGVSQWIYNRGQSDLILVVFVDTRNVANQIDP 201

Query: 208 FARKFYLAGRPEMMRRGEWEGRSYRGSSGEKSGNVFSGFADEFLEEAFQVDSGLVRKLKG 267
           FARKFYLAGRPEMMRRGE EGRS+R S+GEKSGNVFSGFADEFLEEAFQVDSGLVR+LKG
Sbjct: 202 FARKFYLAGRPEMMRRGEREGRSFRESTGEKSGNVFSGFADEFLEEAFQVDSGLVRRLKG 261

Query: 268 EDDERDRIVLAEEDFDVLMPERDDQERSRGRYVESETENGFEETVCTLRLKHSIGRSEHA 327
           E+D+RDRIV  EEDFDVLMPERDDQERSRGRYVE E+ENGFEETVCTLRLKH+IGRSEHA
Sbjct: 262 ENDDRDRIVFTEEDFDVLMPERDDQERSRGRYVEKESENGFEETVCTLRLKHNIGRSEHA 321

Query: 328 DVFNTRGGRISTANFNNLPFLRQVRLSAERGVLYSKAISAPHYTVNAHTLAYATRGSARV 387
           DVFN RGGRISTANFNNLPFLRQVRLSAERGVLYS AISAPHYTVNAHT+AYATRGSARV
Sbjct: 322 DVFNPRGGRISTANFNNLPFLRQVRLSAERGVLYSNAISAPHYTVNAHTVAYATRGSARV 381

Query: 388 QVVDNYGQSVFDGEVREGQVVMIPQNFVVMTKAGDRGFEWIAFKTNDNAITNLLAGRVSQ 447
           QV DNYGQSVFDGEVREGQV++IPQNFVVMT+A DRGFEWIAFKTNDNAITNLLAGRVSQ
Sbjct: 382 QVADNYGQSVFDGEVREGQVLVIPQNFVVMTRASDRGFEWIAFKTNDNAITNLLAGRVSQ 441

Query: 448 MRLLPLGVVSNMYKISREEAQRLKYGQPEMRIFSPGRTQ 487
           MRLLPLGV+SNMY+ISREEAQRLKYGQPEMRIFSPGR+Q
Sbjct: 442 MRLLPLGVLSNMYRISREEAQRLKYGQPEMRIFSPGRSQ 480

BLAST of CSPI02G10300 vs. ExPASy TrEMBL
Match: A0A6J1KVP5 (11S globulin subunit beta-like OS=Cucurbita maxima OX=3661 GN=LOC111499130 PE=3 SV=1)

HSP 1 Score: 757.7 bits (1955), Expect = 3.0e-215
Identity = 374/462 (80.95%), Postives = 417/462 (90.26%), Query Frame = 0

Query: 28  LFIHGCLSQTDQFPWSWGFQGSEARQQHRFHSPKPCHLENLRAQDPVRRIEAEGGFTELW 87
           +FI+ C +Q + FP    FQG EA QQHRFHSP+ C LENLRAQ+PVRR EAE G TE+W
Sbjct: 76  VFINVCFAQFEHFPQE--FQGGEAMQQHRFHSPRACRLENLRAQEPVRRAEAEAGSTEVW 135

Query: 88  EPNSEELQCAGVNMVRHTIRPRGLLLPGFTNAPKLVFVVQGTGIRGVAMPGCPETYQTDL 147
           +  SEE QCAGVNM+RH IR RGLLLPGF+NAPKL+FVVQGTGIRGVA+PGC ETYQTDL
Sbjct: 136 DQYSEEFQCAGVNMLRHKIRSRGLLLPGFSNAPKLIFVVQGTGIRGVALPGCAETYQTDL 195

Query: 148 RRSQSSGN-FRDQHQKIRQFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQID 207
           RRSQS+G+ FRDQHQKIRQFREGDLLVVPAGVSHW+YNRGQSDLILIVF DTRNVANQID
Sbjct: 196 RRSQSAGSAFRDQHQKIRQFREGDLLVVPAGVSHWIYNRGQSDLILIVFTDTRNVANQID 255

Query: 208 PFARKFYLAGRPEMMRRG--EWEGRSYRGSSGEKSGNVFSGFADEFLEEAFQVDSGLVRK 267
           P+ RKF+LAGRPEM+ RG  E EGRS RGS GEKSGN+FSGFADE LEEAF+VDSGLVR+
Sbjct: 256 PYLRKFFLAGRPEMVERGAEEMEGRSRRGSVGEKSGNMFSGFADELLEEAFKVDSGLVRR 315

Query: 268 LKGEDDERDRIVLAEEDFDVLMPERDDQERSRGRYVESETENGFEETVCTLRLKHSIGRS 327
           LKGEDDERDRIV  +EDFDVL+PERD+QERSRGRY+ESE+ENGFEET+CTLRLKH+IGRS
Sbjct: 316 LKGEDDERDRIVQVDEDFDVLLPERDEQERSRGRYIESESENGFEETICTLRLKHNIGRS 375

Query: 328 EHADVFNTRGGRISTANFNNLPFLRQVRLSAERGVLYSKAISAPHYTVNAHTLAYATRGS 387
           E ADVFN RGGRISTAN++NLPFLRQVRLSAERGVLYS A+ APHYTVN+HT+ YATRGS
Sbjct: 376 ERADVFNPRGGRISTANYHNLPFLRQVRLSAERGVLYSNALVAPHYTVNSHTVVYATRGS 435

Query: 388 ARVQVVDNYGQSVFDGEVREGQVVMIPQNFVVMTKAGDRGFEWIAFKTNDNAITNLLAGR 447
           ARVQVVD+ GQSVF+GE+REGQV+MIPQNFVVM +A D+GFEWIAFKTNDNAITNLLAGR
Sbjct: 436 ARVQVVDDSGQSVFNGELREGQVLMIPQNFVVMKQASDKGFEWIAFKTNDNAITNLLAGR 495

Query: 448 VSQMRLLPLGVVSNMYKISREEAQRLKYGQPEMRIFSPGRTQ 487
            SQMR+ PLGV+SNMY+ISREEAQRLKYGQ EMRI SPGR+Q
Sbjct: 496 ASQMRMFPLGVLSNMYRISREEAQRLKYGQQEMRILSPGRSQ 535

BLAST of CSPI02G10300 vs. NCBI nr
Match: KAE8651811.1 (hypothetical protein Csa_006622 [Cucumis sativus])

HSP 1 Score: 933.3 bits (2411), Expect = 8.3e-268
Identity = 458/460 (99.57%), Postives = 460/460 (100.00%), Query Frame = 0

Query: 28  LFIHGCLSQTDQFPWSWGFQGSEARQQHRFHSPKPCHLENLRAQDPVRRIEAEGGFTELW 87
           +FIHGCLSQTDQFPWSWGFQGSEARQQHRFHSPKPCHLENLRAQDPVRRIEAEGGFTELW
Sbjct: 14  VFIHGCLSQTDQFPWSWGFQGSEARQQHRFHSPKPCHLENLRAQDPVRRIEAEGGFTELW 73

Query: 88  EPNSEELQCAGVNMVRHTIRPRGLLLPGFTNAPKLVFVVQGTGIRGVAMPGCPETYQTDL 147
           EPNSEELQCAGVNMVRHTIRPRGLLLPGFTNAPKLVFVVQGTGIRGVAMPGCPETYQTDL
Sbjct: 74  EPNSEELQCAGVNMVRHTIRPRGLLLPGFTNAPKLVFVVQGTGIRGVAMPGCPETYQTDL 133

Query: 148 RRSQSSGNFRDQHQKIRQFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQIDP 207
           RRSQSSGNFRDQHQKIRQFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQIDP
Sbjct: 134 RRSQSSGNFRDQHQKIRQFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQIDP 193

Query: 208 FARKFYLAGRPEMMRRGEWEGRSYRGSSGEKSGNVFSGFADEFLEEAFQVDSGLVRKLKG 267
           FARKFYLAGRPEMMRRGEWEGRSYRGSSGEKSGNVFSGFADEFLEEAFQVDSGLVRKLKG
Sbjct: 194 FARKFYLAGRPEMMRRGEWEGRSYRGSSGEKSGNVFSGFADEFLEEAFQVDSGLVRKLKG 253

Query: 268 EDDERDRIVLAEEDFDVLMPERDDQERSRGRYVESETENGFEETVCTLRLKHSIGRSEHA 327
           EDDERDRIVLAEEDFDVLMPERDDQERSRGRYVESETENGFEETVCTLRLKHSIGRSEHA
Sbjct: 254 EDDERDRIVLAEEDFDVLMPERDDQERSRGRYVESETENGFEETVCTLRLKHSIGRSEHA 313

Query: 328 DVFNTRGGRISTANFNNLPFLRQVRLSAERGVLYSKAISAPHYTVNAHTLAYATRGSARV 387
           DVFNTRGGRISTANFNNLPFLRQVRLSAERGVLY+KAISAPHYTVNAHTLAYATRGSARV
Sbjct: 314 DVFNTRGGRISTANFNNLPFLRQVRLSAERGVLYNKAISAPHYTVNAHTLAYATRGSARV 373

Query: 388 QVVDNYGQSVFDGEVREGQVVMIPQNFVVMTKAGDRGFEWIAFKTNDNAITNLLAGRVSQ 447
           QVVDNYGQSVFDGEVREGQVVMIPQNFVVMTKAGDRGFEWIAFKTNDNAITNLLAGRVSQ
Sbjct: 374 QVVDNYGQSVFDGEVREGQVVMIPQNFVVMTKAGDRGFEWIAFKTNDNAITNLLAGRVSQ 433

Query: 448 MRLLPLGVVSNMYKISREEAQRLKYGQPEMRIFSPGRTQR 488
           MRLLPLGVVSNMYKISREEAQRLKYGQPEMRIFSPGRTQR
Sbjct: 434 MRLLPLGVVSNMYKISREEAQRLKYGQPEMRIFSPGRTQR 473

BLAST of CSPI02G10300 vs. NCBI nr
Match: XP_004141669.3 (11S globulin subunit beta [Cucumis sativus])

HSP 1 Score: 869.0 bits (2244), Expect = 1.9e-248
Identity = 418/459 (91.07%), Postives = 446/459 (97.17%), Query Frame = 0

Query: 28  LFIHGCLSQTDQFPWSWGFQGSEARQQHRFHSPKPCHLENLRAQDPVRRIEAEGGFTELW 87
           +FI+GCLSQTDQFPWSWGFQGSEARQQHRFHSPK CHLENLRA++P RRIEAE G+TELW
Sbjct: 14  VFINGCLSQTDQFPWSWGFQGSEARQQHRFHSPKACHLENLRAEEPARRIEAEAGYTELW 73

Query: 88  EPNSEELQCAGVNMVRHTIRPRGLLLPGFTNAPKLVFVVQGTGIRGVAMPGCPETYQTDL 147
           EPN+EE QCAGVNMVRHTIRP+GLLLPGFTNAPKL+FVVQGTGIRGVAMPGCPETY+TDL
Sbjct: 74  EPNNEEFQCAGVNMVRHTIRPKGLLLPGFTNAPKLIFVVQGTGIRGVAMPGCPETYETDL 133

Query: 148 RRSQSSGNFRDQHQKIRQFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQIDP 207
           RRSQSSGNFRDQHQKIR+FREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQIDP
Sbjct: 134 RRSQSSGNFRDQHQKIREFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQIDP 193

Query: 208 FARKFYLAGRPEMMRRGEWEGRSYRGSSGEKSGNVFSGFADEFLEEAFQVDSGLVRKLKG 267
           FARKFYLAGRPEMMRRGE EGRS+R S+GEKSGNVFSGFADEFLEEA Q+DSGLVR+LKG
Sbjct: 194 FARKFYLAGRPEMMRRGEREGRSFRESTGEKSGNVFSGFADEFLEEALQIDSGLVRRLKG 253

Query: 268 EDDERDRIVLAEEDFDVLMPERDDQERSRGRYVESETENGFEETVCTLRLKHSIGRSEHA 327
           E DERDRIV AEEDFDVLMPE+DDQERSRGRY+E E+ENGFEETVCTLRLKH+IGRSEHA
Sbjct: 254 ESDERDRIVFAEEDFDVLMPEKDDQERSRGRYIEKESENGFEETVCTLRLKHNIGRSEHA 313

Query: 328 DVFNTRGGRISTANFNNLPFLRQVRLSAERGVLYSKAISAPHYTVNAHTLAYATRGSARV 387
           DVFN RGGR+STANFNNLPFLRQVRLSAERGVLYS AISAPHYTVNAHT+AYATRGSARV
Sbjct: 314 DVFNPRGGRLSTANFNNLPFLRQVRLSAERGVLYSNAISAPHYTVNAHTVAYATRGSARV 373

Query: 388 QVVDNYGQSVFDGEVREGQVVMIPQNFVVMTKAGDRGFEWIAFKTNDNAITNLLAGRVSQ 447
           QVVDNYGQ+VFDGEVREGQV++IPQNFVVMT+A +RGFEWIAFKTNDNAITNLLAGRVSQ
Sbjct: 374 QVVDNYGQAVFDGEVREGQVLVIPQNFVVMTRASERGFEWIAFKTNDNAITNLLAGRVSQ 433

Query: 448 MRLLPLGVVSNMYKISREEAQRLKYGQPEMRIFSPGRTQ 487
           MRLLPLGV+SNMY+ISREEAQRLKYGQPEMRIFSPGR+Q
Sbjct: 434 MRLLPLGVLSNMYRISREEAQRLKYGQPEMRIFSPGRSQ 472

BLAST of CSPI02G10300 vs. NCBI nr
Match: XP_008462393.1 (PREDICTED: 11S globulin subunit beta-like [Cucumis melo])

HSP 1 Score: 865.5 bits (2235), Expect = 2.1e-247
Identity = 418/459 (91.07%), Postives = 444/459 (96.73%), Query Frame = 0

Query: 28  LFIHGCLSQTDQFPWSWGFQGSEARQQHRFHSPKPCHLENLRAQDPVRRIEAEGGFTELW 87
           +FI+GCLSQTDQFPWSWGFQGSEARQQHRFHSPK CHLENLRA++P RRIEAE G+TELW
Sbjct: 14  VFINGCLSQTDQFPWSWGFQGSEARQQHRFHSPKACHLENLRAEEPARRIEAEAGYTELW 73

Query: 88  EPNSEELQCAGVNMVRHTIRPRGLLLPGFTNAPKLVFVVQGTGIRGVAMPGCPETYQTDL 147
           EPN+EE QCAGVNMVRHTIRP+GLLLPGFTNAPKL+FVVQG GIRGVAMPGCPETY+TDL
Sbjct: 74  EPNNEEFQCAGVNMVRHTIRPKGLLLPGFTNAPKLIFVVQGAGIRGVAMPGCPETYETDL 133

Query: 148 RRSQSSGNFRDQHQKIRQFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQIDP 207
           RRSQSSGNFRDQHQKIR FREGDLLV+PAGVS W+YNRGQSDLIL+VFVDTRNVANQIDP
Sbjct: 134 RRSQSSGNFRDQHQKIRHFREGDLLVLPAGVSQWIYNRGQSDLILVVFVDTRNVANQIDP 193

Query: 208 FARKFYLAGRPEMMRRGEWEGRSYRGSSGEKSGNVFSGFADEFLEEAFQVDSGLVRKLKG 267
           FARKFYLAGRPEMMRRGE EGRS+R S+GEKSGNVFSGFADEFLEEAFQVDSGLVR+LKG
Sbjct: 194 FARKFYLAGRPEMMRRGEREGRSFRESTGEKSGNVFSGFADEFLEEAFQVDSGLVRRLKG 253

Query: 268 EDDERDRIVLAEEDFDVLMPERDDQERSRGRYVESETENGFEETVCTLRLKHSIGRSEHA 327
           E+D+RDRIV  EEDFDVLMPERDDQERSRGRYVE E+ENGFEETVCTLRLKH+IGRSEHA
Sbjct: 254 ENDDRDRIVFTEEDFDVLMPERDDQERSRGRYVEKESENGFEETVCTLRLKHNIGRSEHA 313

Query: 328 DVFNTRGGRISTANFNNLPFLRQVRLSAERGVLYSKAISAPHYTVNAHTLAYATRGSARV 387
           DVFN RGGRISTANFNNLPFLRQVRLSAERGVLYS AISAPHYTVNAHT+AYATRGSARV
Sbjct: 314 DVFNPRGGRISTANFNNLPFLRQVRLSAERGVLYSNAISAPHYTVNAHTVAYATRGSARV 373

Query: 388 QVVDNYGQSVFDGEVREGQVVMIPQNFVVMTKAGDRGFEWIAFKTNDNAITNLLAGRVSQ 447
           QVVDNYGQSVFDGEVREGQV++IPQNFVVMT+A DRGFEWIAFKTNDNAITNLLAGRVSQ
Sbjct: 374 QVVDNYGQSVFDGEVREGQVLVIPQNFVVMTRASDRGFEWIAFKTNDNAITNLLAGRVSQ 433

Query: 448 MRLLPLGVVSNMYKISREEAQRLKYGQPEMRIFSPGRTQ 487
           MRLLPLGV+SNMY+ISREEAQRLKYGQPEMRIFSPGR+Q
Sbjct: 434 MRLLPLGVLSNMYRISREEAQRLKYGQPEMRIFSPGRSQ 472

BLAST of CSPI02G10300 vs. NCBI nr
Match: KAA0059455.1 (11S globulin subunit beta-like [Cucumis melo var. makuwa] >TYK03869.1 11S globulin subunit beta-like [Cucumis melo var. makuwa])

HSP 1 Score: 864.0 bits (2231), Expect = 6.2e-247
Identity = 417/459 (90.85%), Postives = 443/459 (96.51%), Query Frame = 0

Query: 28  LFIHGCLSQTDQFPWSWGFQGSEARQQHRFHSPKPCHLENLRAQDPVRRIEAEGGFTELW 87
           +FI+GCLSQTDQFPWSWGFQGSEARQQHRFHSPK CHLENLRA++P RRIEAE G+TELW
Sbjct: 22  VFINGCLSQTDQFPWSWGFQGSEARQQHRFHSPKACHLENLRAEEPARRIEAEAGYTELW 81

Query: 88  EPNSEELQCAGVNMVRHTIRPRGLLLPGFTNAPKLVFVVQGTGIRGVAMPGCPETYQTDL 147
           EPN+EE QCAGVNMVRHTIRP+GLLLPGFTNAPKL+FVVQG GIRGVAMPGCPETY+TDL
Sbjct: 82  EPNNEEFQCAGVNMVRHTIRPKGLLLPGFTNAPKLIFVVQGAGIRGVAMPGCPETYETDL 141

Query: 148 RRSQSSGNFRDQHQKIRQFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQIDP 207
           RRSQSSGNFRDQHQKIR FREGDLLV+PAGVS W+YNRGQSDLIL+VFVDTRNVANQIDP
Sbjct: 142 RRSQSSGNFRDQHQKIRHFREGDLLVLPAGVSQWIYNRGQSDLILVVFVDTRNVANQIDP 201

Query: 208 FARKFYLAGRPEMMRRGEWEGRSYRGSSGEKSGNVFSGFADEFLEEAFQVDSGLVRKLKG 267
           FARKFYLAGRPEMMRRGE EGRS+R S+GEKSGNVFSGFADEFLEEAFQVDSGLVR+LKG
Sbjct: 202 FARKFYLAGRPEMMRRGEREGRSFRESTGEKSGNVFSGFADEFLEEAFQVDSGLVRRLKG 261

Query: 268 EDDERDRIVLAEEDFDVLMPERDDQERSRGRYVESETENGFEETVCTLRLKHSIGRSEHA 327
           E+D+RDRIV  EEDFDVLMPERDDQERSRGRYVE E+ENGFEETVCTLRLKH+IGRSEHA
Sbjct: 262 ENDDRDRIVFTEEDFDVLMPERDDQERSRGRYVEKESENGFEETVCTLRLKHNIGRSEHA 321

Query: 328 DVFNTRGGRISTANFNNLPFLRQVRLSAERGVLYSKAISAPHYTVNAHTLAYATRGSARV 387
           DVFN RGGRISTANFNNLPFLRQVRLSAERGVLYS AISAPHYTVNAHT+AYATRGSARV
Sbjct: 322 DVFNPRGGRISTANFNNLPFLRQVRLSAERGVLYSNAISAPHYTVNAHTVAYATRGSARV 381

Query: 388 QVVDNYGQSVFDGEVREGQVVMIPQNFVVMTKAGDRGFEWIAFKTNDNAITNLLAGRVSQ 447
           QV DNYGQSVFDGEVREGQV++IPQNFVVMT+A DRGFEWIAFKTNDNAITNLLAGRVSQ
Sbjct: 382 QVADNYGQSVFDGEVREGQVLVIPQNFVVMTRASDRGFEWIAFKTNDNAITNLLAGRVSQ 441

Query: 448 MRLLPLGVVSNMYKISREEAQRLKYGQPEMRIFSPGRTQ 487
           MRLLPLGV+SNMY+ISREEAQRLKYGQPEMRIFSPGR+Q
Sbjct: 442 MRLLPLGVLSNMYRISREEAQRLKYGQPEMRIFSPGRSQ 480

BLAST of CSPI02G10300 vs. NCBI nr
Match: XP_038875294.1 (11S globulin subunit beta-like [Benincasa hispida])

HSP 1 Score: 853.6 bits (2204), Expect = 8.4e-244
Identity = 415/462 (89.83%), Postives = 443/462 (95.89%), Query Frame = 0

Query: 28  LFIHGCLSQTDQFPWSWGFQGSEARQQHRFHSPKPCHLENLRAQDPVRRIEAEGGFTELW 87
           +FI+GCLSQTDQFPWSWGFQGSE RQQHRFHSPK C LENLRAQ+P RRIEAE G TELW
Sbjct: 64  VFINGCLSQTDQFPWSWGFQGSEQRQQHRFHSPKACRLENLRAQEPARRIEAEAGCTELW 123

Query: 88  EPNSEELQCAGVNMVRHTIRPRGLLLPGFTNAPKLVFVVQGTGIRGVAMPGCPETYQTDL 147
           +PN+EE QCAGVNMVRHTIRPRGLLLPGFTNAPKL+FVVQG+GIR VA+PGCPETYQTDL
Sbjct: 124 DPNNEEFQCAGVNMVRHTIRPRGLLLPGFTNAPKLIFVVQGSGIRSVAIPGCPETYQTDL 183

Query: 148 RRSQSSG-NFRDQHQKIRQFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQID 207
           RRSQS+G +FRDQHQKIR+FREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQID
Sbjct: 184 RRSQSAGSSFRDQHQKIREFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQID 243

Query: 208 PFARKFYLAGRPEMMRR--GEWEGRSYRGSSGEKSGNVFSGFADEFLEEAFQVDSGLVRK 267
           PFARKFYLAGRPEMM+R  GEWEGRS RGS+GEKSGN+FSGFADEFLEEAFQVDSGLVR+
Sbjct: 244 PFARKFYLAGRPEMMQRGAGEWEGRSRRGSAGEKSGNIFSGFADEFLEEAFQVDSGLVRR 303

Query: 268 LKGEDDERDRIVLAEEDFDVLMPERDDQERSRGRYVESETENGFEETVCTLRLKHSIGRS 327
           LKGEDDERDRIV  ++DFDVLMPERDDQERSRGRY+ESE+ENGFEETVCTLRLKH+IGRS
Sbjct: 304 LKGEDDERDRIVQVDQDFDVLMPERDDQERSRGRYIESESENGFEETVCTLRLKHAIGRS 363

Query: 328 EHADVFNTRGGRISTANFNNLPFLRQVRLSAERGVLYSKAISAPHYTVNAHTLAYATRGS 387
           EHADVFN RGGRISTANFNNLPFLRQVRLSAERGVLYS AISAPHYTVNAHT+AY TRGS
Sbjct: 364 EHADVFNPRGGRISTANFNNLPFLRQVRLSAERGVLYSNAISAPHYTVNAHTVAYVTRGS 423

Query: 388 ARVQVVDNYGQSVFDGEVREGQVVMIPQNFVVMTKAGDRGFEWIAFKTNDNAITNLLAGR 447
           ARVQVVDNYGQ VFDGEVREGQV+M+PQNFVVMT+A DRGFEWIAFKTNDNAITNLLAGR
Sbjct: 424 ARVQVVDNYGQLVFDGEVREGQVLMVPQNFVVMTRASDRGFEWIAFKTNDNAITNLLAGR 483

Query: 448 VSQMRLLPLGVVSNMYKISREEAQRLKYGQPEMRIFSPGRTQ 487
           VSQMRLLP+GV+SNMY+ISREEAQRLKYGQPEMRIFSPGR+Q
Sbjct: 484 VSQMRLLPVGVLSNMYRISREEAQRLKYGQPEMRIFSPGRSQ 525

BLAST of CSPI02G10300 vs. TAIR 10
Match: AT1G03890.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 335.5 bits (859), Expect = 7.2e-92
Identity = 179/438 (40.87%), Postives = 253/438 (57.76%), Query Frame = 0

Query: 46  FQGSEARQQHRFHSPKPCHLENLRAQDPVRRIEAEGGFTELWEPNSEELQCAGVNMVRHT 105
           F G+EARQ+     P  CH   + +  P +  + E G  E+W+  S EL+CAGV + R T
Sbjct: 20  FHGAEARQREA-PFPNACHFSQINSLAPAQATKFEAGQMEVWDHMSPELRCAGVTVARIT 79

Query: 106 IRPRGLLLPGFTNAPKLVFVVQGTGIRGVAMPGCPETYQTDLRRSQSSG-------NFRD 165
           ++P  + LP F + P L +VVQG G+ G    GCPET+  ++  S   G        F D
Sbjct: 80  LQPNSIFLPAFFSPPALAYVVQGEGVMGTIASGCPETF-AEVEGSSGRGGGGDPGRRFED 139

Query: 166 QHQKIRQFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQIDPFARKFYLAGRP 225
            HQK+  FR GD+    AGVS W YNRG SD ++++ +D  N  NQ+D   R F LAG  
Sbjct: 140 MHQKLENFRRGDVFASLAGVSQWWYNRGDSDAVIVIVLDVTNRENQLDQVPRMFQLAG-- 199

Query: 226 EMMRRGEWEGRSYRGSSGEKSGNVFSGFADEFLEEAFQVDSGLVRKLKGEDDERDRIVLA 285
               R + E +     SG    N FSGF    + EAF+++    ++L+ + D R  I+ A
Sbjct: 200 ---SRTQEEEQPLTWPSG---NNAFSGFDPNIIAEAFKINIETAKQLQNQKDNRGNIIRA 259

Query: 286 EEDFDVLMPERDDQERSRGRYVESETENGFEETVCTLRLKHSIGRSEHADVFNTRGGRIS 345
                 ++P   +       + +    NG EET CT ++  +I   E +D F+TR GRIS
Sbjct: 260 NGPLHFVIPPPRE-------WQQDGIANGIEETYCTAKIHENIDDPERSDHFSTRAGRIS 319

Query: 346 TANFNNLPFLRQVRLSAERGVLYSKAISAPHYTVNAHTLAYATRGSARVQVVDNYGQSVF 405
           T N  NLP LR VRL+A RG LYS  +  P +T NAHT+ Y T G A++QVVD+ GQSVF
Sbjct: 320 TLNSLNLPVLRLVRLNALRGYLYSGGMVLPQWTANAHTVLYVTGGQAKIQVVDDNGQSVF 379

Query: 406 DGEVREGQVVMIPQNFVVMTKAGDRGFEWIAFKTNDNAITNLLAGRVSQMRLLPLGVVSN 465
           + +V +GQ+++IPQ F V   AG+ GFEWI+FKTNDNA  N L+G+ S +R +P+ V+  
Sbjct: 380 NEQVGQGQIIVIPQGFAVSKTAGETGFEWISFKTNDNAYINTLSGQTSYLRAVPVDVIKA 439

Query: 466 MYKISREEAQRLKYGQPE 477
            Y ++ EEA+R+K+ Q E
Sbjct: 440 SYGVNEEEAKRIKFSQQE 440

BLAST of CSPI02G10300 vs. TAIR 10
Match: AT1G03880.1 (cruciferin 2 )

HSP 1 Score: 318.9 bits (816), Expect = 6.9e-87
Identity = 177/436 (40.60%), Postives = 244/436 (55.96%), Query Frame = 0

Query: 46  FQGSEARQQHRFHSPKPCHLENLRAQDPVRRIEAEGGFTELWEPNSEELQCAGVNMVRHT 105
           F G  A+Q      P  C L+ L A +P + I++EGG  E+W+ ++ +L+C+G    R  
Sbjct: 18  FNGYTAQQW-----PNECQLDQLNALEPSQIIKSEGGRIEVWDHHAPQLRCSGFAFERFV 77

Query: 106 IRPRGLLLPGFTNAPKLVFVVQGTGIRGVAMPGCPETYQTDLRRSQSSG-----NFRDQH 165
           I P+GL LP F NA KL FVV G G+ G  +PGC ET+       +  G      FRD H
Sbjct: 78  IEPQGLFLPTFLNAGKLTFVVHGRGLMGRVIPGCAETFMESPVFGEGQGQGQSQGFRDMH 137

Query: 166 QKIRQFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQIDPFARKFYLAGRPEM 225
           QK+   R GD +  P+GV+ W YN G   LIL+   D  +  NQ+D   R F +AG    
Sbjct: 138 QKVEHLRCGDTIATPSGVAQWFYNNGNEPLILVAAADLASNQNQLDRNLRPFLIAGNNPQ 197

Query: 226 MRRGEWEGRSYRGSSGEKSGNVFSGFADEFLEEAFQVDSGLVRKLKGEDDERDRIVLAEE 285
            +  EW     +G   +K  N+F+GFA E L +AF+++    ++L+ + D R  IV    
Sbjct: 198 GQ--EW----LQGRKQQKQNNIFNGFAPEILAQAFKINVETAQQLQNQQDNRGNIVKVNG 257

Query: 286 DFDVLMPERDDQERSRGRYVESETENGFEETVCTLRLKHSIGRSEHADVFNTRGGRISTA 345
            F V+ P      R  G     E  NG EET+CT+R   ++     ADV+    G IST 
Sbjct: 258 PFGVIRP---PLRRGEGGQQPHEIANGLEETLCTMRCTENLDDPSDADVYKPSLGYISTL 317

Query: 346 NFNNLPFLRQVRLSAERGVLYSKAISAPHYTVNAHTLAYATRGSARVQVVDNYGQSVFDG 405
           N  NLP LR +RLSA RG +   A+  P + VNA+   Y T G A +Q+V++ G+ VFD 
Sbjct: 318 NSYNLPILRLLRLSALRGSIRKNAMVLPQWNVNANAALYVTNGKAHIQMVNDNGERVFDQ 377

Query: 406 EVREGQVVMIPQNFVVMTKAGDRGFEWIAFKTNDNAITNLLAGRVSQMRLLPLGVVSNMY 465
           E+  GQ++++PQ F VM  A    FEWI FKTN+NA  N LAGR S MR LPL V++N Y
Sbjct: 378 EISSGQLLVVPQGFSVMKHAIGEQFEWIEFKTNENAQVNTLAGRTSVMRGLPLEVITNGY 437

Query: 466 KISREEAQRLKYGQPE 477
           +IS EEA+R+K+   E
Sbjct: 438 QISPEEAKRVKFSTIE 439

BLAST of CSPI02G10300 vs. TAIR 10
Match: AT5G44120.3 (RmlC-like cupins superfamily protein )

HSP 1 Score: 306.6 bits (784), Expect = 3.6e-83
Identity = 164/447 (36.69%), Postives = 251/447 (56.15%), Query Frame = 0

Query: 45  GFQGSEARQQHRFHSPKPCHLENLRAQDPVRRIEAEGGFTELWEPNSEELQCAGVNMVRH 104
           G+   + +Q  +F  P  C L+ L A +P   +++E G  E+W+ ++ +L+C+GV+  R+
Sbjct: 20  GYAAQQGQQGQQF--PNECQLDQLNALEPSHVLKSEAGRIEVWDHHAPQLRCSGVSFARY 79

Query: 105 TIRPRGLLLPGFTNAPKLVFVVQGTGIRGVAMPGCPETYQTD------LRRSQSSGNFRD 164
            I  +GL LP F N  KL FV +G G+ G  +PGC ET+Q              S  FRD
Sbjct: 80  IIESKGLYLPSFFNTAKLSFVAKGRGLMGKVIPGCAETFQDSSEFQPRFEGQGQSQRFRD 139

Query: 165 QHQKIRQFREGDLLVVPAGVSHWMYNRGQSDLILIVFVDTRNVANQIDPFARKFYLAGRP 224
            HQK+   R GD +    GV+ W YN GQ  L+++   D  +  NQ+D   R FYLAG  
Sbjct: 140 MHQKVEHIRSGDTIATTPGVAQWFYNDGQEPLVIVSVFDLASHQNQLDRNPRPFYLAGNN 199

Query: 225 EMMRRGEWEGRSY-RGSSGEKSGNVFSGFADEFLEEAFQVDSGLVRKLKGEDDERDRIVL 284
                   +G+ + +G   +   N+F+GF  E + +A ++D    ++L+ +DD R  IV 
Sbjct: 200 P-------QGQVWLQGREQQPQKNIFNGFGPEVIAQALKIDLQTAQQLQNQDDNRGNIVR 259

Query: 285 AEEDFDVLMP--------ERDDQERSRGRYVESETENGFEETVCTLRLKHSIGRSEHADV 344
            +  F V+ P        E +++E   GR+      NG EET+C+ R   ++     ADV
Sbjct: 260 VQGPFGVIRPPLRGQRPQEEEEEEGRHGRH-----GNGLEETICSARCTDNLDDPSRADV 319

Query: 345 FNTRGGRISTANFNNLPFLRQVRLSAERGVLYSKAISAPHYTVNAHTLAYATRGSARVQV 404
           +  + G IST N  +LP LR +RLSA RG +   A+  P +  NA+ + Y T G A++Q+
Sbjct: 320 YKPQLGYISTLNSYDLPILRFIRLSALRGSIRQNAMVLPQWNANANAILYVTDGEAQIQI 379

Query: 405 VDNYGQSVFDGEVREGQVVMIPQNFVVMTKAGDRGFEWIAFKTNDNAITNLLAGRVSQMR 464
           V++ G  VFDG+V +GQ++ +PQ F V+ +A    F+W+ FKTN NA  N LAGR S +R
Sbjct: 380 VNDNGNRVFDGQVSQGQLIAVPQGFSVVKRATSNRFQWVEFKTNANAQINTLAGRTSVLR 439

Query: 465 LLPLGVVSNMYKISREEAQRLKYGQPE 477
            LPL V++N ++IS EEA+R+K+   E
Sbjct: 440 GLPLEVITNGFQISPEEARRVKFNTLE 452

BLAST of CSPI02G10300 vs. TAIR 10
Match: AT4G28520.1 (cruciferin 3 )

HSP 1 Score: 291.2 bits (744), Expect = 1.5e-78
Identity = 167/486 (34.36%), Postives = 253/486 (52.06%), Query Frame = 0

Query: 63  CHLENLRAQDPVRRIEAEGGFTELWEPNSEELQCAGVNMVRHTIRPRGLLLPGFTNAPKL 122
           C+L+NL        I++E G  E W+ N  +L+C GV++ R+ I   GL LP F  +PK+
Sbjct: 37  CNLDNLDVLQATETIKSEAGQIEYWDHNHPQLRCVGVSVARYVIEQGGLYLPTFFTSPKI 96

Query: 123 VFVVQGTGIRGVAMPGCPETYQT------------------------------------- 182
            +VVQGTGI G  +PGC ET+                                       
Sbjct: 97  SYVVQGTGISGRVVPGCAETFMDSQPMQGQQQGQPWQGRQGQQGQPWEGQGQQGQQGRQG 156

Query: 183 ----------------------DLRRSQSSGNFRDQHQKIRQFREGDLLVVPAGVSHWMY 242
                                 + +  Q    FRD HQK+   R GD+     G +HW+Y
Sbjct: 157 QPWEGQGQQGQQGRQGQQGQPWEGQGQQGQQGFRDMHQKVEHVRRGDVFANTPGSAHWIY 216

Query: 243 NRGQSDLILIVFVDTRNVANQIDPFARKFYLAGRPEMMRRGEWEGRSYRGSSGEKSGNVF 302
           N G+  L++I  +D  N  NQ+D   R F+LAG  +   +G + G   +    ++  N++
Sbjct: 217 NSGEQPLVIIALLDIANYQNQLDRNPRVFHLAGNNQ---QGGFGGSQQQ----QEQKNLW 276

Query: 303 SGFADEFLEEAFQVDSGLVRKLKGEDDERDRIVLAEEDFDVLMPE-RDDQERSRGRYVES 362
           SGF  + + +A ++D  L ++L+ + D R  IV  +  F V+ P  R   E    R+  S
Sbjct: 277 SGFDAQVIAQALKIDVQLAQQLQNQQDSRGNIVRVKGPFQVVRPPLRQPYESEEWRHPRS 336

Query: 363 ETENGFEETVCTLRLKHSIGRSEHADVFNTRGGRISTANFNNLPFLRQVRLSAERGVLYS 422
              NG EET+C++R   +I     ADV+    GR+++ N   LP L  VRLSA RGVL  
Sbjct: 337 PQGNGLEETICSMRSHENIDDPARADVYKPSLGRVTSVNSYTLPILEYVRLSATRGVLQG 396

Query: 423 KAISAPHYTVNAHTLAYATRGSARVQVVDNYGQSVFDGEVREGQVVMIPQNFVVMTKAGD 482
            A+  P Y +NA+ + Y T G  R+QVV++ GQ+V D +V++GQ+V+IPQ F  + ++  
Sbjct: 397 NAMVLPKYNMNANEILYCTGGQGRIQVVNDNGQNVLDQQVQKGQLVVIPQGFAYVVQSHG 456

Query: 483 RGFEWIAFKTNDNAITNLLAGRVSQMRLLPLGVVSNMYKISREEAQRLKYGQPEMRIF-S 488
             FEWI+FKTN+NA+ + LAGR S +R LPL V+SN ++IS EEA+++K+   E  +  +
Sbjct: 457 NKFEWISFKTNENAMISTLAGRTSLLRALPLEVISNGFQISPEEARKIKFNTLETTLTRA 515

BLAST of CSPI02G10300 vs. TAIR 10
Match: AT5G44120.2 (RmlC-like cupins superfamily protein )

HSP 1 Score: 240.4 bits (612), Expect = 3.1e-63
Identity = 133/359 (37.05%), Postives = 199/359 (55.43%), Query Frame = 0

Query: 133 GVAMPGCPETYQTD------LRRSQSSGNFRDQHQKIRQFREGDLLVVPAGVSHWMYNRG 192
           G  +PGC ET+Q              S  FRD HQK+   R GD +    GV+ W YN G
Sbjct: 2   GKVIPGCAETFQDSSEFQPRFEGQGQSQRFRDMHQKVEHIRSGDTIATTPGVAQWFYNDG 61

Query: 193 QSDLILIVFVDTRNVANQIDPFARKFYLAGRPEMMRRGEWEGRSY-RGSSGEKSGNVFSG 252
           Q  L+++   D  +  NQ+D   R FYLAG          +G+ + +G   +   N+F+G
Sbjct: 62  QEPLVIVSVFDLASHQNQLDRNPRPFYLAGNNP-------QGQVWLQGREQQPQKNIFNG 121

Query: 253 FADEFLEEAFQVDSGLVRKLKGEDDERDRIVLAEEDFDVLMP--------ERDDQERSRG 312
           F  E + +A ++D    ++L+ +DD R  IV  +  F V+ P        E +++E   G
Sbjct: 122 FGPEVIAQALKIDLQTAQQLQNQDDNRGNIVRVQGPFGVIRPPLRGQRPQEEEEEEGRHG 181

Query: 313 RYVESETENGFEETVCTLRLKHSIGRSEHADVFNTRGGRISTANFNNLPFLRQVRLSAER 372
           R+      NG EET+C+ R   ++     ADV+  + G IST N  +LP LR +RLSA R
Sbjct: 182 RH-----GNGLEETICSARCTDNLDDPSRADVYKPQLGYISTLNSYDLPILRFIRLSALR 241

Query: 373 GVLYSKAISAPHYTVNAHTLAYATRGSARVQVVDNYGQSVFDGEVREGQVVMIPQNFVVM 432
           G +   A+  P +  NA+ + Y T G A++Q+V++ G  VFDG+V +GQ++ +PQ F V+
Sbjct: 242 GSIRQNAMVLPQWNANANAILYVTDGEAQIQIVNDNGNRVFDGQVSQGQLIAVPQGFSVV 301

Query: 433 TKAGDRGFEWIAFKTNDNAITNLLAGRVSQMRLLPLGVVSNMYKISREEAQRLKYGQPE 477
            +A    F+W+ FKTN NA  N LAGR S +R LPL V++N ++IS EEA+R+K+   E
Sbjct: 302 KRATSNRFQWVEFKTNANAQINTLAGRTSVLRGLPLEVITNGFQISPEEARRVKFNTLE 348

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P137447.2e-21478.4511S globulin subunit beta OS=Cucurbita maxima OX=3661 PE=1 SV=1[more]
B5KVH48.2e-11747.5111S globulin seed storage protein 1 OS=Carya illinoinensis OX=32201 PE=1 SV=1[more]
A0A1L6K3711.0e-11447.2311S globulin OS=Juglans nigra OX=16719 PE=1 SV=1[more]
P098006.5e-11444.83Legumin B OS=Gossypium hirsutum OX=3635 GN=LEGB PE=2 SV=1[more]
Q2TPW58.5e-11447.1011S globulin seed storage protein Jug r 4 OS=Juglans regia OX=51240 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LNN61.5e-28699.59Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G174130 PE=3 SV=1[more]
A0A0A0K9P52.1e-24890.85Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G452090 PE=3 SV=1[more]
A0A1S3CIE11.0e-24791.0711S globulin subunit beta-like OS=Cucumis melo OX=3656 GN=LOC103500763 PE=3 SV=1[more]
A0A5D3C0J23.0e-24790.8511S globulin subunit beta-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A6J1KVP53.0e-21580.9511S globulin subunit beta-like OS=Cucurbita maxima OX=3661 GN=LOC111499130 PE=3 ... [more]
Match NameE-valueIdentityDescription
KAE8651811.18.3e-26899.57hypothetical protein Csa_006622 [Cucumis sativus][more]
XP_004141669.31.9e-24891.0711S globulin subunit beta [Cucumis sativus][more]
XP_008462393.12.1e-24791.07PREDICTED: 11S globulin subunit beta-like [Cucumis melo][more]
KAA0059455.16.2e-24790.8511S globulin subunit beta-like [Cucumis melo var. makuwa] >TYK03869.1 11S globul... [more]
XP_038875294.18.4e-24489.8311S globulin subunit beta-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G03890.17.2e-9240.87RmlC-like cupins superfamily protein [more]
AT1G03880.16.9e-8740.60cruciferin 2 [more]
AT5G44120.33.6e-8336.69RmlC-like cupins superfamily protein [more]
AT4G28520.11.5e-7834.36cruciferin 3 [more]
AT5G44120.23.1e-6337.05RmlC-like cupins superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR00604411-S seed storage protein, plantPRINTSPR0043911SGLOBULINcoord: 318..335
score: 35.28
coord: 445..462
score: 38.47
coord: 405..420
score: 52.6
coord: 387..403
score: 57.93
coord: 423..441
score: 48.97
coord: 341..361
score: 50.57
IPR006045Cupin 1SMARTSM00835Cupin_1_3coord: 66..263
e-value: 7.2E-35
score: 131.8
coord: 319..468
e-value: 4.3E-51
score: 185.7
IPR006045Cupin 1PFAMPF00190Cupin_1coord: 68..218
e-value: 3.4E-28
score: 98.1
coord: 326..467
e-value: 4.1E-30
score: 104.4
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 322..487
e-value: 6.1E-70
score: 235.8
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 62..321
e-value: 3.6E-104
score: 349.7
NoneNo IPR availablePANTHERPTHR31189OS03G0336100 PROTEIN-RELATEDcoord: 28..485
NoneNo IPR availablePANTHERPTHR31189:SF48LEGUMIN Bcoord: 28..485
NoneNo IPR availableCDDcd02242cupin_11S_legumin_Ncoord: 65..280
e-value: 9.52033E-102
score: 301.812
NoneNo IPR availableCDDcd02243cupin_11S_legumin_Ccoord: 328..482
e-value: 3.82843E-80
score: 244.306
IPR02237911-S seed storage protein, conserved sitePROSITEPS0030511S_SEED_STORAGEcoord: 306..328
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 28..476

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G10300.1CSPI02G10300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding
molecular_function GO:0045735 nutrient reservoir activity