CSPI02G08330 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G08330
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	protein PHYTOCHROME KINASE SUBSTRATE 1
Location	Chr2: 7822847 .. 7824669 (+)
RNA-Seq Expression	CSPI02G08330
Synteny	CSPI02G08330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAATGGTCCTGCCTTCATAATGTCTTGACAATCTCTCCCAACTTTTCTTCTTATAGAACCAAACTTTGTTCCTCATTTCTGAATTCTCAAATCTTATTATTTACCACTCTTTGATCAGCTCTCTGTCTTCTCTTTGGTGTCTCCTTTTGTTTTGGGATCCAATTTTCGAATTCAATGGACATCTTCACCTCCATATCATCTAAAACATTGCCTTTTGATACCCATATTGATAGCAATAATAGTTTGGGAGTTTATGGCGACACCTCCTTCTCGTCTTACTTGTCTGCAAAAGAAGACCATGACTTCATCCGCAAACTCGCTGAGTCAACTCGTTACCTAAAAAGCCCCAACATGATTCCTTGTAGCAGAGGCGGTGAAGATGGAGAGATTGGAATATTTGGAGCTGAAAAGTACTTCAATGACGGAATGGAAGATGAGAGCACTCAAAGATCAAGTAACAATCATCCATCGTCTCAAAAGCTTGACAAACTTATTGTTGCTCATATGGAGGAAGCCTTGAAATTGCCAAAGCCGAGGCTTGGAACTCCAAGTGTTGGATCGGAATCCAGTAGCGTCAACAGCCAAAGACCTTTGCTGAAGATCGTTAAAAGCACAACAACAACAAGTGCAATAGCAACAACAATTGCCAGTAACAGTTATTCGCTCCAGAAGAGGAGTAGTAACAACAACAAGAGCTTTCTATCCAATACACTTGGCTATTGCATGTGCTGTACAAGCAACAAAAAAAGTACTGCAGTTGAAGACGTGGGTGAAATCAGTTTCAGCAATGCAATCACAACAAACCCAACACGAAATAATATTAATAATATATTGGATCGAGAAGCGCCTTCGTTTCGAGGCTTTCCAACTGCAGCATCATCACTGAAGATGGTCCATTTACAAGAACCTGAAGAAGTAGTTGAAAGAAAGTCGTTAGAGGTTTTCGGTTCGCCTATGACGGGAAGGCTCAGAAACAATAAACCAATGAGCCTGGAAAAAAGGCTAACCATGCTTTCTTGGGATAAAACAAATAACCACAGCACCACCTTAGGATCAGGAATGTTTTACAACGAGGACGAGGTGAATAGCGATTGTAGCTCCGACTTGTTTGAGATTGAAAGCCTTACCAAGCAAACCAACCCATTCCACTCTCCAACTGCCTCTTGTTATGCGCCCAGTGAAGCTAGCGTTGACTGGAGCGTCGTCACTGCCAGTGCTCTAGACTTCGACGAACGGCGCCCCTCCACTACCAGTCCAGCCAGAGTGGTGCCACCACCACCAATGAGGGTTAATGTGCACAAAGAGGTTGTCGTTGTCCCGAAGAGGCGACCGAGTAGTATTTTAGGGTGTAAAAGTGAGAAAGCAGTAAGAGTTGCTGAAGGTAATAATAAGTATGAACGCAAAATGGGTGGTAACAAGTCTAACAACTCTGACTATCTTCTCATGGAACAACGTAACTCCGAGTCGTTGATGGCAGTTAAAAGGTTTGAAGATGAGACCAAAGTCGGAGGGTTCAGTTTCAAATCGCAAGGGTCGTCGTCGATGTTGCCTATTCCCCTGCCTCTGCCACATCATGGTCTCGCCACACGCTCGCTTCCTCGCCCTTATTCCCCAAGGCTTACCAATATAACCTTCAATATGCAGTGAGATGATTGCATGTTATCTTCATATTTCCATAAGTACATTAAACATTTGAACTATACTTATTTACTCTACTCTACTATAAAATAGGATATTTTCCTATTCACTGCTATTCCAAATTTGAATATACCTTTGGTACGAGTGAGGGGTATGTATACATTATTATTCTAAACTCTGGTGAC

mRNA sequence

GAATGGTCCTGCCTTCATAATGTCTTGACAATCTCTCCCAACTTTTCTTCTTATAGAACCAAACTTTGTTCCTCATTTCTGAATTCTCAAATCTTATTATTTACCACTCTTTGATCAGCTCTCTGTCTTCTCTTTGGTGTCTCCTTTTGTTTTGGGATCCAATTTTCGAATTCAATGGACATCTTCACCTCCATATCATCTAAAACATTGCCTTTTGATACCCATATTGATAGCAATAATAGTTTGGGAGTTTATGGCGACACCTCCTTCTCGTCTTACTTGTCTGCAAAAGAAGACCATGACTTCATCCGCAAACTCGCTGAGTCAACTCGTTACCTAAAAAGCCCCAACATGATTCCTTGTAGCAGAGGCGGTGAAGATGGAGAGATTGGAATATTTGGAGCTGAAAAGTACTTCAATGACGGAATGGAAGATGAGAGCACTCAAAGATCAAGTAACAATCATCCATCGTCTCAAAAGCTTGACAAACTTATTGTTGCTCATATGGAGGAAGCCTTGAAATTGCCAAAGCCGAGGCTTGGAACTCCAAGTGTTGGATCGGAATCCAGTAGCGTCAACAGCCAAAGACCTTTGCTGAAGATCGTTAAAAGCACAACAACAACAAGTGCAATAGCAACAACAATTGCCAGTAACAGTTATTCGCTCCAGAAGAGGAGTAGTAACAACAACAAGAGCTTTCTATCCAATACACTTGGCTATTGCATGTGCTGTACAAGCAACAAAAAAAGTACTGCAGTTGAAGACGTGGGTGAAATCAGTTTCAGCAATGCAATCACAACAAACCCAACACGAAATAATATTAATAATATATTGGATCGAGAAGCGCCTTCGTTTCGAGGCTTTCCAACTGCAGCATCATCACTGAAGATGGTCCATTTACAAGAACCTGAAGAAGTAGTTGAAAGAAAGTCGTTAGAGGTTTTCGGTTCGCCTATGACGGGAAGGCTCAGAAACAATAAACCAATGAGCCTGGAAAAAAGGCTAACCATGCTTTCTTGGGATAAAACAAATAACCACAGCACCACCTTAGGATCAGGAATGTTTTACAACGAGGACGAGGTGAATAGCGATTGTAGCTCCGACTTGTTTGAGATTGAAAGCCTTACCAAGCAAACCAACCCATTCCACTCTCCAACTGCCTCTTGTTATGCGCCCAGTGAAGCTAGCGTTGACTGGAGCGTCGTCACTGCCAGTGCTCTAGACTTCGACGAACGGCGCCCCTCCACTACCAGTCCAGCCAGAGTGGTGCCACCACCACCAATGAGGGTTAATGTGCACAAAGAGGTTGTCGTTGTCCCGAAGAGGCGACCGAGTAGTATTTTAGGGTGTAAAAGTGAGAAAGCAGTAAGAGTTGCTGAAGGTAATAATAAGTATGAACGCAAAATGGGTGGTAACAAGTCTAACAACTCTGACTATCTTCTCATGGAACAACGTAACTCCGAGTCGTTGATGGCAGTTAAAAGGTTTGAAGATGAGACCAAAGTCGGAGGGTTCAGTTTCAAATCGCAAGGGTCGTCGTCGATGTTGCCTATTCCCCTGCCTCTGCCACATCATGGTCTCGCCACACGCTCGCTTCCTCGCCCTTATTCCCCAAGGCTTACCAATATAACCTTCAATATGCAGTGAGATGATTGCATGTTATCTTCATATTTCCATAAGTACATTAAACATTTGAACTATACTTATTTACTCTACTCTACTATAAAATAGGATATTTTCCTATTCACTGCTATTCCAAATTTGAATATACCTTTGGTACGAGTGAGGGGTATGTATACATTATTATTCTAAACTCTGGTGAC

Coding sequence (CDS)

ATGGACATCTTCACCTCCATATCATCTAAAACATTGCCTTTTGATACCCATATTGATAGCAATAATAGTTTGGGAGTTTATGGCGACACCTCCTTCTCGTCTTACTTGTCTGCAAAAGAAGACCATGACTTCATCCGCAAACTCGCTGAGTCAACTCGTTACCTAAAAAGCCCCAACATGATTCCTTGTAGCAGAGGCGGTGAAGATGGAGAGATTGGAATATTTGGAGCTGAAAAGTACTTCAATGACGGAATGGAAGATGAGAGCACTCAAAGATCAAGTAACAATCATCCATCGTCTCAAAAGCTTGACAAACTTATTGTTGCTCATATGGAGGAAGCCTTGAAATTGCCAAAGCCGAGGCTTGGAACTCCAAGTGTTGGATCGGAATCCAGTAGCGTCAACAGCCAAAGACCTTTGCTGAAGATCGTTAAAAGCACAACAACAACAAGTGCAATAGCAACAACAATTGCCAGTAACAGTTATTCGCTCCAGAAGAGGAGTAGTAACAACAACAAGAGCTTTCTATCCAATACACTTGGCTATTGCATGTGCTGTACAAGCAACAAAAAAAGTACTGCAGTTGAAGACGTGGGTGAAATCAGTTTCAGCAATGCAATCACAACAAACCCAACACGAAATAATATTAATAATATATTGGATCGAGAAGCGCCTTCGTTTCGAGGCTTTCCAACTGCAGCATCATCACTGAAGATGGTCCATTTACAAGAACCTGAAGAAGTAGTTGAAAGAAAGTCGTTAGAGGTTTTCGGTTCGCCTATGACGGGAAGGCTCAGAAACAATAAACCAATGAGCCTGGAAAAAAGGCTAACCATGCTTTCTTGGGATAAAACAAATAACCACAGCACCACCTTAGGATCAGGAATGTTTTACAACGAGGACGAGGTGAATAGCGATTGTAGCTCCGACTTGTTTGAGATTGAAAGCCTTACCAAGCAAACCAACCCATTCCACTCTCCAACTGCCTCTTGTTATGCGCCCAGTGAAGCTAGCGTTGACTGGAGCGTCGTCACTGCCAGTGCTCTAGACTTCGACGAACGGCGCCCCTCCACTACCAGTCCAGCCAGAGTGGTGCCACCACCACCAATGAGGGTTAATGTGCACAAAGAGGTTGTCGTTGTCCCGAAGAGGCGACCGAGTAGTATTTTAGGGTGTAAAAGTGAGAAAGCAGTAAGAGTTGCTGAAGGTAATAATAAGTATGAACGCAAAATGGGTGGTAACAAGTCTAACAACTCTGACTATCTTCTCATGGAACAACGTAACTCCGAGTCGTTGATGGCAGTTAAAAGGTTTGAAGATGAGACCAAAGTCGGAGGGTTCAGTTTCAAATCGCAAGGGTCGTCGTCGATGTTGCCTATTCCCCTGCCTCTGCCACATCATGGTCTCGCCACACGCTCGCTTCCTCGCCCTTATTCCCCAAGGCTTACCAATATAACCTTCAATATGCAGTGA

Protein sequence

MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNMIPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKPRLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMVHLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNEDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTSPARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSDYLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSPRLTNITFNMQ*

Homology

[Close]

BLAST of CSPI02G08330 vs. ExPASy Swiss-Prot
Match: Q9SWI1 (Protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Arabidopsis thaliana OX=3702 GN=PKS1 PE=1 SV=2)

HSP 1 Score: 139.4 bits (350), Expect = 1.1e-31
Identity = 155/445 (34.83%), Postives = 216/445 (48.54%), Query Frame = 0


Query: 5   TSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDH-DFIRKLAESTRYLK------- 64
           +S S+    FD   ++N+   +Y   S SSYLS+KED     +KL E ++ L        
Sbjct: 7   SSASTPKTSFDFMKNNNSHSSLY--VSSSSYLSSKEDALVTTKKLMEPSKTLNMSINPKQ 66

Query: 65  ---SPNMIPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEE 124
                      +  ED EIG+FGAEKYFN  M  +S Q SS    ++ ++++ +V   + 
Sbjct: 67  EEFGDEKKMVKKAPEDPEIGVFGAEKYFNGDM--DSDQGSSVLSLTNPEVERTVVDSKQS 126

Query: 125 ALKLPKPRLGTPSVGSESSSVNSQRPLL--KIVKSTTTTSAIATTIASNSYSLQKRSSNN 184
           A    K   GTPSV SE SS NSQ  LL  K+V S  +    +     NS    ++ +NN
Sbjct: 127 A----KKSTGTPSVRSE-SSWNSQSVLLQNKLVNSCNS----SFKEKKNSNGQIQKVTNN 186

Query: 185 NKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFP 244
            KSFL+N LG C C  S+  S  VE+   +  S     +P   NI+ I  R         
Sbjct: 187 KKSFLAN-LG-CKCACSDGDSVDVEEKTSVKRS----ADP---NISVITMRS-------- 246

Query: 245 TAASSLKMVHLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTT 304
           +A  + +++ +Q+ EE+ +RKSLEVFGSP+       K   ++K+L +  W        T
Sbjct: 247 SADMNTELIKIQKQEELSQRKSLEVFGSPVA---IEKKSSVVQKKLPLPPWKSRTEEDDT 306

Query: 305 LGSGMFYNEDEVNSDCSSDLFEIESLTKQTNPF--------HSPTASCYAPSEASVDWSV 364
              G         SD SSDLFEIE LT    PF         SPT  CYAPSE SV+WS+
Sbjct: 307 KSEG---------SDSSSDLFEIEGLTGNPKPFLTRQGSDPASPT--CYAPSEVSVEWSI 366

Query: 365 VTASALDFDERRPSTTSPARVVPPPPM-RVNVHKEVVVVPKRRPSS--------ILGCKS 420
           VTASA DF       TSP R   P  + R+ +  +    P+RR SS        ++ CKS
Sbjct: 367 VTASAADFSVMSECATSPVRRNRPTQIPRIPITAK--SAPQRRKSSSSSGGNGFLMSCKS 402

[Close]

BLAST of CSPI02G08330 vs. ExPASy Swiss-Prot
Match: Q9M9T4 (Protein PHYTOCHROME KINASE SUBSTRATE 2 OS=Arabidopsis thaliana OX=3702 GN=PKS2 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 3.2e-28
Identity = 148/432 (34.26%), Postives = 209/432 (48.38%), Query Frame = 0


Query: 5   TSISSKTLPFDTHI-DSNNSLGVYGDTSFS----SYLSAKEDHDFIRKLAES-TRYLKSP 64
           +S S+  + FD  + ++NNS  +YG  S S    SYL++KED    + L    T  +   
Sbjct: 6   SSSSTPNVSFDFMMNNNNNSNNLYGPFSSSSTSFSYLTSKEDALTQKNLMSGITNDVLGI 65

Query: 65  NMIPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQ-KLDKLIVAHMEEALKL 124
           N     +  ED EI +FGAEKYFN  M+ + + R  +  P  +  ++++ V   + +   
Sbjct: 66  N----KKASEDLEISVFGAEKYFNGDMDSDHSPRLVSPLPDPEVPIERIFVGPKQSS--- 125

Query: 125 PKPRLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNN----- 184
            K    TPS+ SE SS NSQ  LL+  K       I    + NSY  +K  S+N+     
Sbjct: 126 -KNSSETPSLRSE-SSWNSQSLLLQ-SKYVEKKKNIKKNSSCNSYFQEKDMSSNHKVSNK 185

Query: 185 KSFLSNTLGYCMCCTSNKKST-AVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFP 244
           KSFL+ TLG C C  SN  S   V+D    S    I T                    F 
Sbjct: 186 KSFLA-TLG-CRCVCSNWSSVDVVDDKRRSSGLKKIKTQ-----------------LSFS 245

Query: 245 TAASSLKMVHLQEPEEVVE-RKSLEVFGSPMTGRLRNNKPMSLEKRLTM--LSWDKTNN- 304
              SS   +H Q+ E ++E RKSLE+FGSP+           +EKR+      W+ +++ 
Sbjct: 246 GDLSSEMKIHQQQQEAMLEQRKSLEIFGSPL-----------IEKRIIQKKFPWEYSSSA 305

Query: 305 HSTTLGSGMFYNEDEVN--SDCSSDLFEIESLTKQTNPFHSPTASC-------YAPSEAS 364
                G  + Y E+E    SD S+DLFEIESLT + NPF +   S        YAPSE S
Sbjct: 306 KKEEHGFSVKYEEEEDGSVSDVSTDLFEIESLTGKANPFLARQGSSDPDSPDGYAPSEVS 365

Query: 365 VDWSVVTASALDFDERRPSTTSPAR-----VVPPPPMRVNVHKEVVVVPKRRPSS----I 402
           + WSVVTAS  DF       TSP +      +P  P+    ++E  + P+RR SS    +
Sbjct: 366 IQWSVVTASVADFSVMSECATSPVKKNRSFQIPRIPIMAKSNRE--IAPQRRKSSSSGLL 395

[Close]

BLAST of CSPI02G08330 vs. ExPASy Swiss-Prot
Match: Q8GXS8 (Protein PHYTOCHROME KINASE SUBSTRATE 3 OS=Arabidopsis thaliana OX=3702 GN=PKS3 PE=2 SV=2)

HSP 1 Score: 72.4 bits (176), Expect = 1.6e-11
Identity = 112/382 (29.32%), Postives = 158/382 (41.36%), Query Frame = 0


Query: 65  RGGEDGEIGIFGAEKYFN------DGMEDESTQRSSNN---HPSSQKLDKLIVAHMEEAL 124
           +   D EIG+FGAEKYF+      D   D + Q    N   HP++   +     H     
Sbjct: 41  KADNDAEIGVFGAEKYFSMKLDHVDSTADITKQHEKENTHDHPNANP-NPNPNPHPHPHP 100

Query: 125 KLPK--------PRLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKR 184
           +L K         R GTPSV SE SS NSQ  L++I                N+++  K+
Sbjct: 101 QLTKTTSSRSKTSRHGTPSVRSE-SSCNSQTFLMRI----------------NNHNENKQ 160

Query: 185 SSNNNKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAITTNP-----TRNNINNILDR 244
              N+ S    + G   C             G  S    + T+P     +RN+  + +  
Sbjct: 161 RKMNDTSI---SFGGFRC------------YGPCSGVKTVHTDPKNSCKSRNSDRDFVAY 220

Query: 245 EAPSFRGFPTAASSLKMVHLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSW 304
           +A      P      K       E++    SL +         R++  M+LE++L++L+W
Sbjct: 221 DARKHNDKPRLHFEAKKADFHAQEKI----SLPI--------QRSDIAMNLERKLSLLTW 280

Query: 305 D--------KTNNHSTT-LGSGMFYN--EDEVNSDCSSDLFEIESLTKQTNPFHSPTASC 364
           D        K NNH+     S M  N  E+E  S  SSDLFEIE++          T+S 
Sbjct: 281 DAIPNQLSTKNNNHNNNGNNSSMSSNTQEEETASVASSDLFEIENI----------TSSV 340

Query: 365 YAPSEASVDWSVVTASAL------DFDERRPSTTSPARVVPPPPMRVNVHKEVVVVPKRR 407
           Y PSEAS+ WSVVT S        DFD  +  T +   V   P          V+  K R
Sbjct: 341 YEPSEASIGWSVVTGSMADQSVISDFDMMKRVTRNGPVVKTKP----------VIGEKVR 357

[Close]

BLAST of CSPI02G08330 vs. ExPASy TrEMBL
Match: A0A0A0LKV2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G108600 PE=4 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 1.7e-274
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0


Query: 1   MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60
           MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60

Query: 61  IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP
Sbjct: 61  IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120

Query: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTL 180
           RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTL
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTL 180

Query: 181 GYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMV 240
           GYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMV
Sbjct: 181 GYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMV 240

Query: 241 HLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE 300
           HLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE
Sbjct: 241 HLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE 300

Query: 301 DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS 360
           DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS
Sbjct: 301 DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS 360

Query: 361 PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSD 420
           PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSD
Sbjct: 361 PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSD 420

Query: 421 YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSP 480
           YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSP
Sbjct: 421 YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSP 480

Query: 481 RLTNITFNMQ 491
           RLTNITFNMQ
Sbjct: 481 RLTNITFNMQ 490

[Close]

BLAST of CSPI02G08330 vs. ExPASy TrEMBL
Match: A0A5A7SNC2 (Protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold745G00080 PE=4 SV=1)

HSP 1 Score: 876.7 bits (2264), Expect = 4.5e-251
Identity = 456/491 (92.87%), Postives = 468/491 (95.32%), Query Frame = 0


Query: 1   MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60
           MDIFTSISSKTLPFDTHIDSNN+LGVYGDTSFSSYL+AKEDHDFIRKL ESTRYLKSP M
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLTAKEDHDFIRKLTESTRYLKSPIM 60

Query: 61  IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IP SRGGEDGEIGIFGAEKYFN GMEDESTQRS NNHPSSQK DKLIVAHMEEALKLPKP
Sbjct: 61  IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQRSGNNHPSSQKFDKLIVAHMEEALKLPKP 120

Query: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQK-RSSNNNKSFLSNT 180
           RLGTPSVGSESSSVNSQRPLLKIVKSTTTT+AIATTIA+NSYSLQK RSSNNNKSFLSNT
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTTAIATTIANNSYSLQKRRSSNNNKSFLSNT 180

Query: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKM 240
           LGYCMCCTSNKKSTAVEDVGEISFSNA+TTNPTRNNINNILDRE PSFRGFPTAASS+KM
Sbjct: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSMKM 240

Query: 241 VHLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYN 300
           VHLQEPEEVVERKSLEVFGSP+TGRLR+NKP+SLEKRLTMLSWDKTNNHSTTLGSGMFYN
Sbjct: 241 VHLQEPEEVVERKSLEVFGSPVTGRLRSNKPISLEKRLTMLSWDKTNNHSTTLGSGMFYN 300

Query: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTT 360
           EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPS T
Sbjct: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSIT 360

Query: 361 SPARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNS 420
           SPARVVPPPPMRVNVHKEV VVPKRRPSSILGC SEKAVRVAEGNNKY+ KM GNKSNNS
Sbjct: 361 SPARVVPPPPMRVNVHKEVFVVPKRRPSSILGCNSEKAVRVAEGNNKYDHKMSGNKSNNS 420

Query: 421 DYLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYS 480
           DYL MEQR+SESLMAVKRFEDETKVGG SFKSQGSSSML  PL  P   L TRSLPRPYS
Sbjct: 421 DYLHMEQRDSESLMAVKRFEDETKVGGLSFKSQGSSSML--PLAPPARALGTRSLPRPYS 480

Query: 481 PRLTNITFNMQ 491
           PRLTN+TFNMQ
Sbjct: 481 PRLTNMTFNMQ 489

[Close]

BLAST of CSPI02G08330 vs. ExPASy TrEMBL
Match: A0A1S3B4P0 (protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Cucumis melo OX=3656 GN=LOC103485779 PE=4 SV=1)

HSP 1 Score: 876.7 bits (2264), Expect = 4.5e-251
Identity = 456/491 (92.87%), Postives = 468/491 (95.32%), Query Frame = 0


Query: 1   MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60
           MDIFTSISSKTLPFDTHIDSNN+LGVYGDTSFSSYL+AKEDHDFIRKL ESTRYLKSP M
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLTAKEDHDFIRKLTESTRYLKSPIM 60

Query: 61  IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IP SRGGEDGEIGIFGAEKYFN GMEDESTQRS NNHPSSQK DKLIVAHMEEALKLPKP
Sbjct: 61  IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQRSGNNHPSSQKFDKLIVAHMEEALKLPKP 120

Query: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQK-RSSNNNKSFLSNT 180
           RLGTPSVGSESSSVNSQRPLLKIVKSTTTT+AIATTIA+NSYSLQK RSSNNNKSFLSNT
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTTAIATTIANNSYSLQKRRSSNNNKSFLSNT 180

Query: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKM 240
           LGYCMCCTSNKKSTAVEDVGEISFSNA+TTNPTRNNINNILDRE PSFRGFPTAASS+KM
Sbjct: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSMKM 240

Query: 241 VHLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYN 300
           VHLQEPEEVVERKSLEVFGSP+TGRLR+NKP+SLEKRLTMLSWDKTNNHSTTLGSGMFYN
Sbjct: 241 VHLQEPEEVVERKSLEVFGSPVTGRLRSNKPISLEKRLTMLSWDKTNNHSTTLGSGMFYN 300

Query: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTT 360
           EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPS T
Sbjct: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSIT 360

Query: 361 SPARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNS 420
           SPARVVPPPPMRVNVHKEV VVPKRRPSSILGC SEKAVRVAEGNNKY+ KM GNKSNNS
Sbjct: 361 SPARVVPPPPMRVNVHKEVFVVPKRRPSSILGCNSEKAVRVAEGNNKYDHKMSGNKSNNS 420

Query: 421 DYLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYS 480
           DYL MEQR+SESLMAVKRFEDETKVGG SFKSQGSSSML  PL  P   L TRSLPRPYS
Sbjct: 421 DYLHMEQRDSESLMAVKRFEDETKVGGLSFKSQGSSSML--PLAPPARALGTRSLPRPYS 480

Query: 481 PRLTNITFNMQ 491
           PRLTN+TFNMQ
Sbjct: 481 PRLTNMTFNMQ 489

[Close]

BLAST of CSPI02G08330 vs. ExPASy TrEMBL
Match: A0A6J1K8F0 (protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493179 PE=4 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 2.3e-90
Identity = 231/430 (53.72%), Postives = 271/430 (63.02%), Query Frame = 0


Query: 70  GEIGIFGAEKYFNDGME---DESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKPRLGTPS 129
           GEIG+F AEKYFN G++   D       NN P S+ LD        +    P P L TPS
Sbjct: 16  GEIGVFRAEKYFNGGLDIHVDSPRINPPNNKP-SKNLD-------PDGPFTPNPWLATPS 75

Query: 130 VGSES--SSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTLG-YC 189
           V SES  ++ N+Q+PLL   K                       ++N K FLS+ LG YC
Sbjct: 76  VASESCTTTTNTQKPLLNTHK-----------------------THNTKGFLSHALGYYC 135

Query: 190 MCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMVHLQ 249
           MC + + K T  +D+GEISFS  +TT PT +  +N           F     SLK+VH Q
Sbjct: 136 MCYSRSDKPT--DDIGEISFSKPVTTAPTASFRDN----------SFTFQNGSLKLVHFQ 195

Query: 250 EPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNEDEV 309
           EPE  VERKSLEVFGSP+ GR R NKP SLEK+L MLSWD TN+ S         N DE 
Sbjct: 196 EPE--VERKSLEVFGSPVIGRHR-NKPFSLEKKLAMLSWDHTNSSSNN-------NNDEA 255

Query: 310 NSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTSPAR 369
           NSDCSSDLFEIESLTKQTNPF SPT S YAPSEASV+WSVVTASALDFDERR STTSPAR
Sbjct: 256 NSDCSSDLFEIESLTKQTNPFQSPTPSYYAPSEASVEWSVVTASALDFDERRISTTSPAR 315

Query: 370 VV-PPPPMRVNVHKEV-VVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSDY 429
           V  PPPP RV V+++V VVV KRR  S+LGCKSEKAVRVAE N+   RK+ G  + NS  
Sbjct: 316 VAPPPPPRRVGVNEQVAVVVQKRRSGSLLGCKSEKAVRVAEDNSN-GRKISGKVNFNSSS 375

Query: 430 LLMEQRNSESL-MAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSP 489
             +      S+ ++V R EDET+V  FSF+SQ    +    LP  HH LATRSLPRPYSP
Sbjct: 376 SSVSVSVPVSVPVSVNRLEDETRVRSFSFRSQAPQPL----LPSSHHALATRSLPRPYSP 387

Query: 490 RLTNITFNMQ 491
           RL+NITFN+Q
Sbjct: 436 RLSNITFNIQ 387

[Close]

BLAST of CSPI02G08330 vs. ExPASy TrEMBL
Match: A0A6J1GCF6 (protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452711 PE=4 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 8.3e-88
Identity = 224/428 (52.34%), Postives = 262/428 (61.21%), Query Frame = 0


Query: 70  GEIGIFGAEKYFNDGME---DESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKPRLGTPS 129
           GEIG+F AEKYFN G++   D       NN PS        +    +    P P L TPS
Sbjct: 16  GEIGVFRAEKYFNGGLDIHVDSPRTNPPNNKPS--------INLAPDEPFTPNPWLATPS 75

Query: 130 VGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTLG-YCMC 189
           V SES               TTTT+     +      L    ++N K FLS+ LG YCMC
Sbjct: 76  VASES--------------CTTTTTTTQKPL------LNTHKTHNTKGFLSHALGYYCMC 135

Query: 190 CTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMVHLQEP 249
            + + K +  +D+GEISFS  +TT PT +  +N           F     SLK+VH QEP
Sbjct: 136 YSRSDKPS--DDIGEISFSRPVTTAPTASFRDN----------SFTFQNGSLKLVHFQEP 195

Query: 250 EEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNEDEVNS 309
           E  VERKSLEVFGSP+ GR R NKP SLEK+L MLSWD TNN++        YN DE NS
Sbjct: 196 E--VERKSLEVFGSPVIGRHR-NKPFSLEKKLAMLSWDHTNNNN--------YNNDEANS 255

Query: 310 DCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTSPARVV 369
           DCSSDLFEIESLTKQTNPF SPT S YAPSEASV+WSVVTASALDFDERR STTSPARV 
Sbjct: 256 DCSSDLFEIESLTKQTNPFQSPTPSYYAPSEASVEWSVVTASALDFDERRISTTSPARVA 315

Query: 370 ---PPPPMRVNVHKEV-VVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSDY 429
              PPPP RV V+++V VVV KRR  S+LGCKSEKAVRVAE N+   +  G    N+S  
Sbjct: 316 APPPPPPRRVGVNEQVAVVVQKRRSGSLLGCKSEKAVRVAEDNSNGRKISGKVNFNSSSS 375

Query: 430 LLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSPR 489
            +         ++V RFEDET+V  FSF+SQ      P  LP   H LATRSLPRPYSPR
Sbjct: 376 SVSVSVPVPVSVSVNRFEDETRVRSFSFRSQAP----PPLLPSSRHSLATRSLPRPYSPR 388

[Close]

BLAST of CSPI02G08330 vs. NCBI nr
Match: XP_004138989.1 (protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis sativus] >KGN61382.1 hypothetical protein Csa_006559 [Cucumis sativus])

HSP 1 Score: 954.5 bits (2466), Expect = 3.5e-274
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0


Query: 1   MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60
           MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60

Query: 61  IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP
Sbjct: 61  IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120

Query: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTL 180
           RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTL
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTL 180

Query: 181 GYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMV 240
           GYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMV
Sbjct: 181 GYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMV 240

Query: 241 HLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE 300
           HLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE
Sbjct: 241 HLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE 300

Query: 301 DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS 360
           DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS
Sbjct: 301 DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS 360

Query: 361 PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSD 420
           PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSD
Sbjct: 361 PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSD 420

Query: 421 YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSP 480
           YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSP
Sbjct: 421 YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSP 480

Query: 481 RLTNITFNMQ 491
           RLTNITFNMQ
Sbjct: 481 RLTNITFNMQ 490

[Close]

BLAST of CSPI02G08330 vs. NCBI nr
Match: XP_008441702.1 (PREDICTED: protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis melo] >KAA0032290.1 protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis melo var. makuwa] >TYK13512.1 protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis melo var. makuwa])

HSP 1 Score: 876.7 bits (2264), Expect = 9.3e-251
Identity = 456/491 (92.87%), Postives = 468/491 (95.32%), Query Frame = 0


Query: 1   MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60
           MDIFTSISSKTLPFDTHIDSNN+LGVYGDTSFSSYL+AKEDHDFIRKL ESTRYLKSP M
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLTAKEDHDFIRKLTESTRYLKSPIM 60

Query: 61  IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IP SRGGEDGEIGIFGAEKYFN GMEDESTQRS NNHPSSQK DKLIVAHMEEALKLPKP
Sbjct: 61  IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQRSGNNHPSSQKFDKLIVAHMEEALKLPKP 120

Query: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQK-RSSNNNKSFLSNT 180
           RLGTPSVGSESSSVNSQRPLLKIVKSTTTT+AIATTIA+NSYSLQK RSSNNNKSFLSNT
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTTAIATTIANNSYSLQKRRSSNNNKSFLSNT 180

Query: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKM 240
           LGYCMCCTSNKKSTAVEDVGEISFSNA+TTNPTRNNINNILDRE PSFRGFPTAASS+KM
Sbjct: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSMKM 240

Query: 241 VHLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYN 300
           VHLQEPEEVVERKSLEVFGSP+TGRLR+NKP+SLEKRLTMLSWDKTNNHSTTLGSGMFYN
Sbjct: 241 VHLQEPEEVVERKSLEVFGSPVTGRLRSNKPISLEKRLTMLSWDKTNNHSTTLGSGMFYN 300

Query: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTT 360
           EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPS T
Sbjct: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSIT 360

Query: 361 SPARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNS 420
           SPARVVPPPPMRVNVHKEV VVPKRRPSSILGC SEKAVRVAEGNNKY+ KM GNKSNNS
Sbjct: 361 SPARVVPPPPMRVNVHKEVFVVPKRRPSSILGCNSEKAVRVAEGNNKYDHKMSGNKSNNS 420

Query: 421 DYLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYS 480
           DYL MEQR+SESLMAVKRFEDETKVGG SFKSQGSSSML  PL  P   L TRSLPRPYS
Sbjct: 421 DYLHMEQRDSESLMAVKRFEDETKVGGLSFKSQGSSSML--PLAPPARALGTRSLPRPYS 480

Query: 481 PRLTNITFNMQ 491
           PRLTN+TFNMQ
Sbjct: 481 PRLTNMTFNMQ 489

[Close]

BLAST of CSPI02G08330 vs. NCBI nr
Match: XP_038889450.1 (protein PHYTOCHROME KINASE SUBSTRATE 1-like [Benincasa hispida])

HSP 1 Score: 661.0 bits (1704), Expect = 8.0e-186
Identity = 381/510 (74.71%), Postives = 420/510 (82.35%), Query Frame = 0


Query: 1   MDIFTSISSKTLPFDTHID-SNNSLGVYGDTSFSSYLSAKEDHDF-IRKLAESTRYLKSP 60
           MDIF+SIS+KTLPFDTHID +NN++GVYGD S     S KEDH F I KL ESTRYLKS 
Sbjct: 1   MDIFSSISTKTLPFDTHIDNNNNNIGVYGDPS-----SYKEDHTFIIHKLTESTRYLKSS 60

Query: 61  NMIPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIV--AH-----M 120
           N IP S G EDGEIGIFGAEKYFN G+E++ T+       S+QKLDKL+V  AH     +
Sbjct: 61  NTIPGSGGHEDGEIGIFGAEKYFNGGIENDGTR----TERSAQKLDKLLVLAAHQQHNLL 120

Query: 121 EEALKLPKPRLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKR--SS 180
           EE+LKLPKPRLGTPSVGSESSS+NSQRPLL +VK+       +TTIA+N+YSLQKR  SS
Sbjct: 121 EESLKLPKPRLGTPSVGSESSSINSQRPLLNVVKN-------STTIANNNYSLQKRSSSS 180

Query: 181 NNNKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAITTNPTR-----NNINNILDREA 240
           N+NKSFLSNT GYCMCC+S++KS AVEDVGEISFSNA+TT PTR     NN NNI +RE 
Sbjct: 181 NHNKSFLSNTFGYCMCCSSSEKS-AVEDVGEISFSNAVTTKPTRSNNNNNNNNNIFERET 240

Query: 241 PSFRGFPTAA--SSLKMVHLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSW 300
           PSFRGFPTAA  SSLKM+H QEPEEV ERKSLEVFGSP+ GRLRNNKP+SLEKRL MLSW
Sbjct: 241 PSFRGFPTAASSSSLKMIHFQEPEEVGERKSLEVFGSPVMGRLRNNKPISLEKRLAMLSW 300

Query: 301 DKTNNHSTTLGSGMFYNEDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWS 360
           D TNN++ +LGSG+FYNEDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWS
Sbjct: 301 DHTNNNNNSLGSGVFYNEDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWS 360

Query: 361 VVTASALDFDERRPSTTSPARVVPPP-PMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVA 420
           VVTASALDFDERR STTSP RVV P  PMRVNV+KE  VV KRRPSSILGCKSEKAVRVA
Sbjct: 361 VVTASALDFDERRLSTTSPTRVVAPSLPMRVNVNKE--VVQKRRPSSILGCKSEKAVRVA 420

Query: 421 EGNNKYERKMGGNKSNNSDYLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSS-SMLPI 480
           E NNKY RKM G KSN  DY      NSESL+AVKR EDETKVGG SF+SQGSS S+LP+
Sbjct: 421 EDNNKYGRKMNG-KSNYCDY------NSESLVAVKRSEDETKVGGLSFRSQGSSASLLPL 480

Query: 481 PLPLPHHGLATRSLPRPYSPRLTNITFNMQ 491
           P P PH  LATR LPRPYSPRLTNI+FN+Q
Sbjct: 481 P-PPPHRALATRPLPRPYSPRLTNISFNIQ 483

[Close]

BLAST of CSPI02G08330 vs. NCBI nr
Match: XP_022998592.1 (protein PHYTOCHROME KINASE SUBSTRATE 1-like [Cucurbita maxima])

HSP 1 Score: 342.8 bits (878), Expect = 4.8e-90
Identity = 231/430 (53.72%), Postives = 271/430 (63.02%), Query Frame = 0


Query: 70  GEIGIFGAEKYFNDGME---DESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKPRLGTPS 129
           GEIG+F AEKYFN G++   D       NN P S+ LD        +    P P L TPS
Sbjct: 16  GEIGVFRAEKYFNGGLDIHVDSPRINPPNNKP-SKNLD-------PDGPFTPNPWLATPS 75

Query: 130 VGSES--SSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTLG-YC 189
           V SES  ++ N+Q+PLL   K                       ++N K FLS+ LG YC
Sbjct: 76  VASESCTTTTNTQKPLLNTHK-----------------------THNTKGFLSHALGYYC 135

Query: 190 MCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMVHLQ 249
           MC + + K T  +D+GEISFS  +TT PT +  +N           F     SLK+VH Q
Sbjct: 136 MCYSRSDKPT--DDIGEISFSKPVTTAPTASFRDN----------SFTFQNGSLKLVHFQ 195

Query: 250 EPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNEDEV 309
           EPE  VERKSLEVFGSP+ GR R NKP SLEK+L MLSWD TN+ S         N DE 
Sbjct: 196 EPE--VERKSLEVFGSPVIGRHR-NKPFSLEKKLAMLSWDHTNSSSNN-------NNDEA 255

Query: 310 NSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTSPAR 369
           NSDCSSDLFEIESLTKQTNPF SPT S YAPSEASV+WSVVTASALDFDERR STTSPAR
Sbjct: 256 NSDCSSDLFEIESLTKQTNPFQSPTPSYYAPSEASVEWSVVTASALDFDERRISTTSPAR 315

Query: 370 VV-PPPPMRVNVHKEV-VVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSDY 429
           V  PPPP RV V+++V VVV KRR  S+LGCKSEKAVRVAE N+   RK+ G  + NS  
Sbjct: 316 VAPPPPPRRVGVNEQVAVVVQKRRSGSLLGCKSEKAVRVAEDNSN-GRKISGKVNFNSSS 375

Query: 430 LLMEQRNSESL-MAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSP 489
             +      S+ ++V R EDET+V  FSF+SQ    +    LP  HH LATRSLPRPYSP
Sbjct: 376 SSVSVSVPVSVPVSVNRLEDETRVRSFSFRSQAPQPL----LPSSHHALATRSLPRPYSP 387

Query: 490 RLTNITFNMQ 491
           RL+NITFN+Q
Sbjct: 436 RLSNITFNIQ 387

[Close]

BLAST of CSPI02G08330 vs. NCBI nr
Match: XP_022949325.1 (protein PHYTOCHROME KINASE SUBSTRATE 1-like [Cucurbita moschata])

HSP 1 Score: 334.3 bits (856), Expect = 1.7e-87
Identity = 224/428 (52.34%), Postives = 262/428 (61.21%), Query Frame = 0


Query: 70  GEIGIFGAEKYFNDGME---DESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKPRLGTPS 129
           GEIG+F AEKYFN G++   D       NN PS        +    +    P P L TPS
Sbjct: 16  GEIGVFRAEKYFNGGLDIHVDSPRTNPPNNKPS--------INLAPDEPFTPNPWLATPS 75

Query: 130 VGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTLG-YCMC 189
           V SES               TTTT+     +      L    ++N K FLS+ LG YCMC
Sbjct: 76  VASES--------------CTTTTTTTQKPL------LNTHKTHNTKGFLSHALGYYCMC 135

Query: 190 CTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMVHLQEP 249
            + + K +  +D+GEISFS  +TT PT +  +N           F     SLK+VH QEP
Sbjct: 136 YSRSDKPS--DDIGEISFSRPVTTAPTASFRDN----------SFTFQNGSLKLVHFQEP 195

Query: 250 EEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNEDEVNS 309
           E  VERKSLEVFGSP+ GR R NKP SLEK+L MLSWD TNN++        YN DE NS
Sbjct: 196 E--VERKSLEVFGSPVIGRHR-NKPFSLEKKLAMLSWDHTNNNN--------YNNDEANS 255

Query: 310 DCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTSPARVV 369
           DCSSDLFEIESLTKQTNPF SPT S YAPSEASV+WSVVTASALDFDERR STTSPARV 
Sbjct: 256 DCSSDLFEIESLTKQTNPFQSPTPSYYAPSEASVEWSVVTASALDFDERRISTTSPARVA 315

Query: 370 ---PPPPMRVNVHKEV-VVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSDY 429
              PPPP RV V+++V VVV KRR  S+LGCKSEKAVRVAE N+   +  G    N+S  
Sbjct: 316 APPPPPPRRVGVNEQVAVVVQKRRSGSLLGCKSEKAVRVAEDNSNGRKISGKVNFNSSSS 375

Query: 430 LLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSPR 489
            +         ++V RFEDET+V  FSF+SQ      P  LP   H LATRSLPRPYSPR
Sbjct: 376 SVSVSVPVPVSVSVNRFEDETRVRSFSFRSQAP----PPLLPSSRHSLATRSLPRPYSPR 388

[Close]

BLAST of CSPI02G08330 vs. TAIR 10
Match: AT2G02950.1 (phytochrome kinase substrate 1 )

HSP 1 Score: 139.4 bits (350), Expect = 7.6e-33
Identity = 155/445 (34.83%), Postives = 216/445 (48.54%), Query Frame = 0


Query: 5   TSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDH-DFIRKLAESTRYLK------- 64
           +S S+    FD   ++N+   +Y   S SSYLS+KED     +KL E ++ L        
Sbjct: 7   SSASTPKTSFDFMKNNNSHSSLY--VSSSSYLSSKEDALVTTKKLMEPSKTLNMSINPKQ 66

Query: 65  ---SPNMIPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEE 124
                      +  ED EIG+FGAEKYFN  M  +S Q SS    ++ ++++ +V   + 
Sbjct: 67  EEFGDEKKMVKKAPEDPEIGVFGAEKYFNGDM--DSDQGSSVLSLTNPEVERTVVDSKQS 126

Query: 125 ALKLPKPRLGTPSVGSESSSVNSQRPLL--KIVKSTTTTSAIATTIASNSYSLQKRSSNN 184
           A    K   GTPSV SE SS NSQ  LL  K+V S  +    +     NS    ++ +NN
Sbjct: 127 A----KKSTGTPSVRSE-SSWNSQSVLLQNKLVNSCNS----SFKEKKNSNGQIQKVTNN 186

Query: 185 NKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFP 244
            KSFL+N LG C C  S+  S  VE+   +  S     +P   NI+ I  R         
Sbjct: 187 KKSFLAN-LG-CKCACSDGDSVDVEEKTSVKRS----ADP---NISVITMRS-------- 246

Query: 245 TAASSLKMVHLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTT 304
           +A  + +++ +Q+ EE+ +RKSLEVFGSP+       K   ++K+L +  W        T
Sbjct: 247 SADMNTELIKIQKQEELSQRKSLEVFGSPVA---IEKKSSVVQKKLPLPPWKSRTEEDDT 306

Query: 305 LGSGMFYNEDEVNSDCSSDLFEIESLTKQTNPF--------HSPTASCYAPSEASVDWSV 364
              G         SD SSDLFEIE LT    PF         SPT  CYAPSE SV+WS+
Sbjct: 307 KSEG---------SDSSSDLFEIEGLTGNPKPFLTRQGSDPASPT--CYAPSEVSVEWSI 366

Query: 365 VTASALDFDERRPSTTSPARVVPPPPM-RVNVHKEVVVVPKRRPSS--------ILGCKS 420
           VTASA DF       TSP R   P  + R+ +  +    P+RR SS        ++ CKS
Sbjct: 367 VTASAADFSVMSECATSPVRRNRPTQIPRIPITAK--SAPQRRKSSSSSGGNGFLMSCKS 402

[Close]

BLAST of CSPI02G08330 vs. TAIR 10
Match: AT1G14280.1 (phytochrome kinase substrate 2 )

HSP 1 Score: 127.9 bits (320), Expect = 2.3e-29
Identity = 148/432 (34.26%), Postives = 209/432 (48.38%), Query Frame = 0


Query: 5   TSISSKTLPFDTHI-DSNNSLGVYGDTSFS----SYLSAKEDHDFIRKLAES-TRYLKSP 64
           +S S+  + FD  + ++NNS  +YG  S S    SYL++KED    + L    T  +   
Sbjct: 6   SSSSTPNVSFDFMMNNNNNSNNLYGPFSSSSTSFSYLTSKEDALTQKNLMSGITNDVLGI 65

Query: 65  NMIPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQ-KLDKLIVAHMEEALKL 124
           N     +  ED EI +FGAEKYFN  M+ + + R  +  P  +  ++++ V   + +   
Sbjct: 66  N----KKASEDLEISVFGAEKYFNGDMDSDHSPRLVSPLPDPEVPIERIFVGPKQSS--- 125

Query: 125 PKPRLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNN----- 184
            K    TPS+ SE SS NSQ  LL+  K       I    + NSY  +K  S+N+     
Sbjct: 126 -KNSSETPSLRSE-SSWNSQSLLLQ-SKYVEKKKNIKKNSSCNSYFQEKDMSSNHKVSNK 185

Query: 185 KSFLSNTLGYCMCCTSNKKST-AVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFP 244
           KSFL+ TLG C C  SN  S   V+D    S    I T                    F 
Sbjct: 186 KSFLA-TLG-CRCVCSNWSSVDVVDDKRRSSGLKKIKTQ-----------------LSFS 245

Query: 245 TAASSLKMVHLQEPEEVVE-RKSLEVFGSPMTGRLRNNKPMSLEKRLTM--LSWDKTNN- 304
              SS   +H Q+ E ++E RKSLE+FGSP+           +EKR+      W+ +++ 
Sbjct: 246 GDLSSEMKIHQQQQEAMLEQRKSLEIFGSPL-----------IEKRIIQKKFPWEYSSSA 305

Query: 305 HSTTLGSGMFYNEDEVN--SDCSSDLFEIESLTKQTNPFHSPTASC-------YAPSEAS 364
                G  + Y E+E    SD S+DLFEIESLT + NPF +   S        YAPSE S
Sbjct: 306 KKEEHGFSVKYEEEEDGSVSDVSTDLFEIESLTGKANPFLARQGSSDPDSPDGYAPSEVS 365

Query: 365 VDWSVVTASALDFDERRPSTTSPAR-----VVPPPPMRVNVHKEVVVVPKRRPSS----I 402
           + WSVVTAS  DF       TSP +      +P  P+    ++E  + P+RR SS    +
Sbjct: 366 IQWSVVTASVADFSVMSECATSPVKKNRSFQIPRIPIMAKSNRE--IAPQRRKSSSSGLL 395

[Close]

BLAST of CSPI02G08330 vs. TAIR 10
Match: AT1G18810.1 (phytochrome kinase substrate-related )

HSP 1 Score: 72.4 bits (176), Expect = 1.1e-12
Identity = 112/382 (29.32%), Postives = 158/382 (41.36%), Query Frame = 0


Query: 65  RGGEDGEIGIFGAEKYFN------DGMEDESTQRSSNN---HPSSQKLDKLIVAHMEEAL 124
           +   D EIG+FGAEKYF+      D   D + Q    N   HP++   +     H     
Sbjct: 41  KADNDAEIGVFGAEKYFSMKLDHVDSTADITKQHEKENTHDHPNANP-NPNPNPHPHPHP 100

Query: 125 KLPK--------PRLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKR 184
           +L K         R GTPSV SE SS NSQ  L++I                N+++  K+
Sbjct: 101 QLTKTTSSRSKTSRHGTPSVRSE-SSCNSQTFLMRI----------------NNHNENKQ 160

Query: 185 SSNNNKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAITTNP-----TRNNINNILDR 244
              N+ S    + G   C             G  S    + T+P     +RN+  + +  
Sbjct: 161 RKMNDTSI---SFGGFRC------------YGPCSGVKTVHTDPKNSCKSRNSDRDFVAY 220

Query: 245 EAPSFRGFPTAASSLKMVHLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSW 304
           +A      P      K       E++    SL +         R++  M+LE++L++L+W
Sbjct: 221 DARKHNDKPRLHFEAKKADFHAQEKI----SLPI--------QRSDIAMNLERKLSLLTW 280

Query: 305 D--------KTNNHSTT-LGSGMFYN--EDEVNSDCSSDLFEIESLTKQTNPFHSPTASC 364
           D        K NNH+     S M  N  E+E  S  SSDLFEIE++          T+S 
Sbjct: 281 DAIPNQLSTKNNNHNNNGNNSSMSSNTQEEETASVASSDLFEIENI----------TSSV 340

Query: 365 YAPSEASVDWSVVTASAL------DFDERRPSTTSPARVVPPPPMRVNVHKEVVVVPKRR 407
           Y PSEAS+ WSVVT S        DFD  +  T +   V   P          V+  K R
Sbjct: 341 YEPSEASIGWSVVTGSMADQSVISDFDMMKRVTRNGPVVKTKP----------VIGEKVR 357

The following BLAST results are available for this feature:

BLAST of CSPI02G08330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SWI1	1.1e-31	34.83	Protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Arabidopsis thaliana OX=3702 GN=PKS1 P...	[more]
Q9M9T4	3.2e-28	34.26	Protein PHYTOCHROME KINASE SUBSTRATE 2 OS=Arabidopsis thaliana OX=3702 GN=PKS2 P...	[more]
Q8GXS8	1.6e-11	29.32	Protein PHYTOCHROME KINASE SUBSTRATE 3 OS=Arabidopsis thaliana OX=3702 GN=PKS3 P...	[more]

BLAST of CSPI02G08330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LKV2	1.7e-274	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G108600 PE=4 SV=1	[more]
A0A5A7SNC2	4.5e-251	92.87	Protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3B4P0	4.5e-251	92.87	protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Cucumis melo OX=3656 GN=LOC103485779 P...	[more]
A0A6J1K8F0	2.3e-90	53.72	protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1GCF6	8.3e-88	52.34	protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]

BLAST of CSPI02G08330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138989.1	3.5e-274	100.00	protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis sativus] >KGN61382.1 hypothetica...	[more]
XP_008441702.1	9.3e-251	92.87	PREDICTED: protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis melo] >KAA0032290.1 p...	[more]
XP_038889450.1	8.0e-186	74.71	protein PHYTOCHROME KINASE SUBSTRATE 1-like [Benincasa hispida]	[more]
XP_022998592.1	4.8e-90	53.72	protein PHYTOCHROME KINASE SUBSTRATE 1-like [Cucurbita maxima]	[more]
XP_022949325.1	1.7e-87	52.34	protein PHYTOCHROME KINASE SUBSTRATE 1-like [Cucurbita moschata]	[more]

BLAST of CSPI02G08330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02950.1	7.6e-33	34.83	phytochrome kinase substrate 1	[more]
AT1G14280.1	2.3e-29	34.26	phytochrome kinase substrate 2	[more]
AT1G18810.1	1.1e-12	29.32	phytochrome kinase substrate-related	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33781:SF4	PROTEIN PHYTOCHROME KINASE SUBSTRATE 1	coord: 3..448
IPR039615	Protein PHYTOCHROME KINASE SUBSTRATE	PANTHER	PTHR33781	PROTEIN PHYTOCHROME KINASE SUBSTRATE 1-RELATED	coord: 3..448

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G08330.1	CSPI02G08330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
biological_process	GO:0009638	phototropism
molecular_function	GO:0016301	kinase activity