CSPI02G08000 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G08000
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Mitotic-spindle organizing protein 1
Location	Chr2: 7330746 .. 7331065 (+)
RNA-Seq Expression	CSPI02G08000
Synteny	CSPI02G08000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCATTGGAATGTTCAAATCCCTGTTTATACCATTGTTAAAATAATTTTAGAAAGACTTGAAGATTTTGTATAACGTAATTTTCTTCTTTATTAGATTATCTAAATGGATCAAGAGGCTGCACGAACTGCTCGAGAATCACTAGACCTTGCATTTCATATGTCTAACGTACTTGATGCTGGGATCGATCGGCACACTCTCTCTGTCCTCATTGCTCTCTGTGACATGGGTGTGAACCCTGAAGCTCTGGCTGCTGTGGTCAAGGAATTACGGCGGGAGCCACCCTCATCAGACACCATTGTATCCATTAACAAGTCATAA

mRNA sequence

ATCATTGGAATGTTCAAATCCCTGTTTATACCATTGTTAAAATAATTTTAGAAAGACTTGAAGATTTTGTATAACGTAATTTTCTTCTTTATTAGATTATCTAAATGGATCAAGAGGCTGCACGAACTGCTCGAGAATCACTAGACCTTGCATTTCATATGTCTAACGTACTTGATGCTGGGATCGATCGGCACACTCTCTCTGTCCTCATTGCTCTCTGTGACATGGGTGTGAACCCTGAAGCTCTGGCTGCTGTGGTCAAGGAATTACGGCGGGAGCCACCCTCATCAGACACCATTGTATCCATTAACAAGTCATAA

Coding sequence (CDS)

ATGGATCAAGAGGCTGCACGAACTGCTCGAGAATCACTAGACCTTGCATTTCATATGTCTAACGTACTTGATGCTGGGATCGATCGGCACACTCTCTCTGTCCTCATTGCTCTCTGTGACATGGGTGTGAACCCTGAAGCTCTGGCTGCTGTGGTCAAGGAATTACGGCGGGAGCCACCCTCATCAGACACCATTGTATCCATTAACAAGTCATAA

Protein sequence

MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPPSSDTIVSINKS*

Homology

[Close]

BLAST of CSPI02G08000 vs. ExPASy Swiss-Prot
Match: Q9M0N8 (Mitotic-spindle organizing protein 1B OS=Arabidopsis thaliana OX=3702 GN=GIP1 PE=1 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 2.8e-21
Identity = 53/71 (74.65%), Postives = 60/71 (84.51%), Query Frame = 0


Query: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRRE-- 60
          MD+EA+RTARESL+L F MSN+LD G+DRHTLSVLIALCD+GVNPEALA VVKELRRE  
Sbjct: 1  MDEEASRTARESLELVFRMSNILDTGLDRHTLSVLIALCDLGVNPEALATVVKELRRESI 60

Query: 61 PPSSDTIVSIN 70
          P S  T  SI+
Sbjct: 61 PDSVTTTPSIH 71

[Close]

BLAST of CSPI02G08000 vs. ExPASy Swiss-Prot
Match: Q9C9T3 (Mitotic-spindle organizing protein 1A OS=Arabidopsis thaliana OX=3702 GN=GIP2 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 2.0e-19
Identity = 46/66 (69.70%), Postives = 57/66 (86.36%), Query Frame = 0


Query: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60
          M+QEAA TARESL+L F MSN+L+ G+DRHTLSVLIALCD+G+NPEALA +VKELRR+  
Sbjct: 1  MNQEAAETARESLELVFRMSNILETGLDRHTLSVLIALCDIGLNPEALATLVKELRRDSA 60

Query: 61 SSDTIV 67
          ++ T V
Sbjct: 61 TTTTTV 66

[Close]

BLAST of CSPI02G08000 vs. ExPASy Swiss-Prot
Match: A9NKD9 (Mitotic-spindle organizing protein 1 OS=Picea sitchensis OX=3332 PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 8.3e-18
Identity = 45/58 (77.59%), Postives = 51/58 (87.93%), Query Frame = 0


Query: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRRE 59
          MD+ AA+ ARESLDLAF +SN L  G+DRHTLS+LIALC+ GVNPEALAAVVKELRRE
Sbjct: 1  MDRTAAQNARESLDLAFSISNFLQTGLDRHTLSILIALCEHGVNPEALAAVVKELRRE 58

[Close]

BLAST of CSPI02G08000 vs. ExPASy Swiss-Prot
Match: B5FXZ4 (Mitotic-spindle organizing protein 1 OS=Taeniopygia guttata OX=59729 GN=mzt1 PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 4.1e-09
Identity = 24/48 (50.00%), Postives = 39/48 (81.25%), Query Frame = 0


Query: 10 RESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRR 58
          RE++D+ F +S +L+ G+D  TLS+ + LC+ G+NPEAL++V+KELR+
Sbjct: 12 RETMDVLFEISRILNTGLDMETLSICVRLCEQGINPEALSSVIKELRK 59

[Close]

BLAST of CSPI02G08000 vs. ExPASy Swiss-Prot
Match: Q5U4M5 (Mitotic-spindle organizing protein 1 OS=Xenopus laevis OX=8355 GN=mzt1 PE=3 SV=2)

HSP 1 Score: 61.2 bits (147), Expect = 5.4e-09
Identity = 27/56 (48.21%), Postives = 43/56 (76.79%), Query Frame = 0


Query: 10 RESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPPSSDTI 66
          RE++D+   +S +L+ G+D  TLS+ + LC+ G+NPEAL++V+KELRR   +SDT+
Sbjct: 12 RETMDVLLEISRLLNTGLDMETLSICVRLCEQGINPEALSSVIKELRR---ASDTL 64

[Close]

BLAST of CSPI02G08000 vs. ExPASy TrEMBL
Match: A0A0A0LMZ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G094370 PE=3 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 4.9e-29
Identity = 70/71 (98.59%), Postives = 70/71 (98.59%), Query Frame = 0


Query: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60
          MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP
Sbjct: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60

Query: 61 SSDTIVSINKS 72
          SSDTIVS NKS
Sbjct: 61 SSDTIVSSNKS 71

[Close]

BLAST of CSPI02G08000 vs. ExPASy TrEMBL
Match: A0A1S3B468 (mitotic-spindle organizing protein 1A-like OS=Cucumis melo OX=3656 GN=LOC103485818 PE=3 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.1e-27
Identity = 67/71 (94.37%), Postives = 69/71 (97.18%), Query Frame = 0


Query: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60
          MDQEAARTARESLDLAFHMSNVLD+GIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP
Sbjct: 1  MDQEAARTARESLDLAFHMSNVLDSGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60

Query: 61 SSDTIVSINKS 72
          SS+T VS NKS
Sbjct: 61 SSETNVSSNKS 71

[Close]

BLAST of CSPI02G08000 vs. ExPASy TrEMBL
Match: A0A6J1I8U7 (mitotic-spindle organizing protein 1B-like OS=Cucurbita maxima OX=3661 GN=LOC111472550 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.7e-26
Identity = 66/71 (92.96%), Postives = 67/71 (94.37%), Query Frame = 0


Query: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60
          MD EAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKEL REPP
Sbjct: 1  MDPEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60

Query: 61 SSDTIVSINKS 72
          SS+TI S NKS
Sbjct: 61 SSETITSGNKS 71

[Close]

BLAST of CSPI02G08000 vs. ExPASy TrEMBL
Match: A0A5D3D1N9 (Mitotic-spindle organizing protein 1A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003990 PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.3e-26
Identity = 66/72 (91.67%), Postives = 70/72 (97.22%), Query Frame = 0


Query: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60
          MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELR+EPP
Sbjct: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRQEPP 60

Query: 61 SSDTIVSI-NKS 72
          +S+TI+S  NKS
Sbjct: 61 ASETIISASNKS 72

[Close]

BLAST of CSPI02G08000 vs. ExPASy TrEMBL
Match: A0A5D3DEX1 (Mitotic-spindle organizing protein 1A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold333G001260 PE=3 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 2.5e-25
Identity = 62/64 (96.88%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60
          MDQEAARTARESLDLAFHMSNVLD+GIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP
Sbjct: 1  MDQEAARTARESLDLAFHMSNVLDSGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60

Query: 61 SSDT 65
          SS+T
Sbjct: 61 SSET 64

[Close]

BLAST of CSPI02G08000 vs. NCBI nr
Match: XP_011649034.1 (mitotic-spindle organizing protein 1A [Cucumis sativus])

HSP 1 Score: 136.3 bits (342), Expect = 1.0e-28
Identity = 70/71 (98.59%), Postives = 70/71 (98.59%), Query Frame = 0


Query: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60
          MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP
Sbjct: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60

Query: 61 SSDTIVSINKS 72
          SSDTIVS NKS
Sbjct: 61 SSDTIVSSNKS 71

[Close]

BLAST of CSPI02G08000 vs. NCBI nr
Match: XP_008441751.1 (PREDICTED: mitotic-spindle organizing protein 1A-like [Cucumis melo] >XP_008441752.1 PREDICTED: mitotic-spindle organizing protein 1A-like [Cucumis melo] >XP_008441753.1 PREDICTED: mitotic-spindle organizing protein 1A-like [Cucumis melo] >XP_008441755.1 PREDICTED: mitotic-spindle organizing protein 1A-like [Cucumis melo] >XP_008441756.1 PREDICTED: mitotic-spindle organizing protein 1A-like [Cucumis melo] >XP_008441757.1 PREDICTED: mitotic-spindle organizing protein 1A-like [Cucumis melo])

HSP 1 Score: 131.0 bits (328), Expect = 4.2e-27
Identity = 67/71 (94.37%), Postives = 69/71 (97.18%), Query Frame = 0


Query: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60
          MDQEAARTARESLDLAFHMSNVLD+GIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP
Sbjct: 1  MDQEAARTARESLDLAFHMSNVLDSGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60

Query: 61 SSDTIVSINKS 72
          SS+T VS NKS
Sbjct: 61 SSETNVSSNKS 71

[Close]

BLAST of CSPI02G08000 vs. NCBI nr
Match: XP_038889535.1 (mitotic-spindle organizing protein 1B [Benincasa hispida] >XP_038889536.1 mitotic-spindle organizing protein 1B [Benincasa hispida])

HSP 1 Score: 130.6 bits (327), Expect = 5.5e-27
Identity = 66/70 (94.29%), Postives = 68/70 (97.14%), Query Frame = 0


Query: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60
          MD EAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP
Sbjct: 1  MDPEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60

Query: 61 SSDTIVSINK 71
          SS+T+VS NK
Sbjct: 61 SSETVVSSNK 70

[Close]

BLAST of CSPI02G08000 vs. NCBI nr
Match: KAE8651738.1 (hypothetical protein Csa_006450 [Cucumis sativus])

HSP 1 Score: 129.8 bits (325), Expect = 9.5e-27
Identity = 65/67 (97.01%), Postives = 67/67 (100.00%), Query Frame = 0


Query: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60
          MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP
Sbjct: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60

Query: 61 SSDTIVS 68
          SSDTI++
Sbjct: 61 SSDTIIA 67

[Close]

BLAST of CSPI02G08000 vs. NCBI nr
Match: XP_022973921.1 (mitotic-spindle organizing protein 1B-like [Cucurbita maxima] >XP_022973922.1 mitotic-spindle organizing protein 1B-like [Cucurbita maxima])

HSP 1 Score: 127.9 bits (320), Expect = 3.6e-26
Identity = 66/71 (92.96%), Postives = 67/71 (94.37%), Query Frame = 0


Query: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60
          MD EAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKEL REPP
Sbjct: 1  MDPEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60

Query: 61 SSDTIVSINKS 72
          SS+TI S NKS
Sbjct: 61 SSETITSGNKS 71

[Close]

BLAST of CSPI02G08000 vs. TAIR 10
Match: AT4G09550.1 (AtGCP3 interacting protein 1 )

HSP 1 Score: 102.1 bits (253), Expect = 2.0e-22
Identity = 53/71 (74.65%), Postives = 60/71 (84.51%), Query Frame = 0


Query: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRRE-- 60
          MD+EA+RTARESL+L F MSN+LD G+DRHTLSVLIALCD+GVNPEALA VVKELRRE  
Sbjct: 1  MDEEASRTARESLELVFRMSNILDTGLDRHTLSVLIALCDLGVNPEALATVVKELRRESI 60

Query: 61 PPSSDTIVSIN 70
          P S  T  SI+
Sbjct: 61 PDSVTTTPSIH 71

[Close]

BLAST of CSPI02G08000 vs. TAIR 10
Match: AT1G73790.1 (Protein of unknown function (DUF3743) )

HSP 1 Score: 95.9 bits (237), Expect = 1.4e-20
Identity = 46/66 (69.70%), Postives = 57/66 (86.36%), Query Frame = 0


Query: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60
          M+QEAA TARESL+L F MSN+L+ G+DRHTLSVLIALCD+G+NPEALA +VKELRR+  
Sbjct: 1  MNQEAAETARESLELVFRMSNILETGLDRHTLSVLIALCDIGLNPEALATLVKELRRDSA 60

Query: 61 SSDTIV 67
          ++ T V
Sbjct: 61 TTTTTV 66

The following BLAST results are available for this feature:

BLAST of CSPI02G08000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M0N8	2.8e-21	74.65	Mitotic-spindle organizing protein 1B OS=Arabidopsis thaliana OX=3702 GN=GIP1 PE...	[more]
Q9C9T3	2.0e-19	69.70	Mitotic-spindle organizing protein 1A OS=Arabidopsis thaliana OX=3702 GN=GIP2 PE...	[more]
A9NKD9	8.3e-18	77.59	Mitotic-spindle organizing protein 1 OS=Picea sitchensis OX=3332 PE=3 SV=1	[more]
B5FXZ4	4.1e-09	50.00	Mitotic-spindle organizing protein 1 OS=Taeniopygia guttata OX=59729 GN=mzt1 PE=...	[more]
Q5U4M5	5.4e-09	48.21	Mitotic-spindle organizing protein 1 OS=Xenopus laevis OX=8355 GN=mzt1 PE=3 SV=2	[more]

BLAST of CSPI02G08000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LMZ6	4.9e-29	98.59	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G094370 PE=3 SV=1	[more]
A0A1S3B468	2.1e-27	94.37	mitotic-spindle organizing protein 1A-like OS=Cucumis melo OX=3656 GN=LOC1034858...	[more]
A0A6J1I8U7	1.7e-26	92.96	mitotic-spindle organizing protein 1B-like OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A5D3D1N9	2.3e-26	91.67	Mitotic-spindle organizing protein 1A-like OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A5D3DEX1	2.5e-25	96.88	Mitotic-spindle organizing protein 1A-like OS=Cucumis melo var. makuwa OX=119469...	[more]

BLAST of CSPI02G08000 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011649034.1	1.0e-28	98.59	mitotic-spindle organizing protein 1A [Cucumis sativus]	[more]
XP_008441751.1	4.2e-27	94.37	PREDICTED: mitotic-spindle organizing protein 1A-like [Cucumis melo] >XP_0084417...	[more]
XP_038889535.1	5.5e-27	94.29	mitotic-spindle organizing protein 1B [Benincasa hispida] >XP_038889536.1 mitoti...	[more]
KAE8651738.1	9.5e-27	97.01	hypothetical protein Csa_006450 [Cucumis sativus]	[more]
XP_022973921.1	3.6e-26	92.96	mitotic-spindle organizing protein 1B-like [Cucurbita maxima] >XP_022973922.1 mi...	[more]

BLAST of CSPI02G08000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G09550.1	2.0e-22	74.65	AtGCP3 interacting protein 1	[more]
AT1G73790.1	1.4e-20	69.70	Protein of unknown function (DUF3743)	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR022214	Mitotic-spindle organizing protein 1	PFAM	PF12554	MOZART1	coord: 10..56 e-value: 2.1E-20 score: 72.2
IPR022214	Mitotic-spindle organizing protein 1	PANTHER	PTHR28520	MITOTIC-SPINDLE ORGANIZING PROTEIN 1	coord: 1..63
None	No IPR available	PANTHER	PTHR28520:SF10	MITOTIC-SPINDLE ORGANIZING PROTEIN 1A-LIKE	coord: 1..63

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G08000.1	CSPI02G08000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0033566	gamma-tubulin complex localization
biological_process	GO:0051415	microtubule nucleation by interphase microtubule organizing center
biological_process	GO:0090307	mitotic spindle assembly
cellular_component	GO:0000931	gamma-tubulin large complex
cellular_component	GO:0031021	interphase microtubule organizing center
cellular_component	GO:0005819	spindle