CSPI02G07750 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G07750
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionAuxin-responsive GH3 family protein
LocationChr2: 6820728 .. 6825736 (-)
RNA-Seq ExpressionCSPI02G07750
SyntenyCSPI02G07750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTAACCCGAATCTTAAAAATAATCTACAAAGATGCCGTCACATGTTGAAGAGAGTTGGTTGGATGAGAGAGATAAGGAAGTACTACAACACATTCAGCATATCACAAGCAATGCTGGCGAAATCCAACGACAAATTCTAAGTGAAATTCTGTCAACGAATGCTAATGTTGAATATTTACAACAACATGGCCTTCATGCCTCTACTGGTTCTTCAACCTTCAAAAAACTTATACCTCTTGTCTCTTACGAACAATTAAAACCTTATATAACTCGTATTGCAGAAGGTGATGATTCCCCAATCCTATGCTCAAACCCCATTACAGCGTTCTTTCTAAGGTGACAAACACACACACACACACACACATATATATTTCATATACTCGCCCTATCTGTTTTTTTGTTTTAAATTAATTCTTTTATATGTCTTGTTGTAGCTCTGGGACATCTGGGGGAGAACCCAAGTTAGTTCCAATATATGAAAAAGAGTTTGAGAGAAGGTTGTCTTTTTTCAACTATTTGATGGCAAGGACGAAAGAATTGTTTCCAAATATCAATTGGCACAAAGGTAAAGCTATGAACTTTCACTTTGCAAAGCCTGATCATAAGACAAAAGCTGGAATTCTTGTTCATACTGTATTTTCAAGACTGCTCAAAAGATCACTAAATCTCAAAAGTGTAGAAAGTGGGAATAACGCAATCCCTGATGACATTCTTCGATGCACGAACACATACCAAAGCTTATACTGTCAGCTTCTTTGTGGGCTCTACCAAAACGATCTAGTTTTTCAAGTTGGTGCAGTTTTGGCTTCTGGGTTGATTCACGTTTTTAAGTTCCTTGAAAATCATTGGGTTGATTTGGTTAGTGATATACGTAGAGGAAGTATTAATAACCCCAAAATTACAGACTTATCACTGAGAGAATCAGTTATGAAGATTCTTGTAAAACCCAATCCTCAACTTGCAGATTTGATTGAAACTGAATGTAGCAAAGGAAAATGGAAAGGAATTGTTCCAAAATTGTGGCCAAACACTAAATATATTAAGGCTATTGCCACAGGAAGCTTGTCACAATACATTCCATTGCTAAATTACTACACAAACAACCTCCCTATATTTTCTGACCATTATGGTTCTACTGAATGTTTCCTTGGCTTGAATCTGGATCCAATATGTGACCCTAATGAAACCTCCTACACTTTGATCCCTACCATGGCGTATTTCGAGTTCTTACCAATAGATACCACAAATATCAATGGCGAAGTGACCCAAGAATTAGTTGATCTTGTTGATGTCAAGTTAGGACAAGAATATGAGCTCGTCATCACCACTTTCGCTGGTAAATGACCTCTTTTCCTTTCTCATACTATATTTATTCAATATTCATATCTGCATAAATTTCAAAGTTCAGTCTCATAACTTCAAATTCTGTTGTGTACGTGTTCGCTAACTTATATAGCTAACGTATATTTTTTGATAAACTCGAATCTTTCAAAAAGAAAATTAACTAATATATTATTAAATGTAAAATTGAACTTATATACGCTATGATCGGAGTAGATATTTGAATTGTGGTGTTTGTTGCATATTGTGTAAGATATCACTTTGCATTTAGATATTCTTCACCCTACGATCACGTTCTTATAGGCCGTATATTCGGAGTTTTGAAACTACAGCCCCGAAATCCACTCGATTTGACATTTTTCTTAATGAGAAGAATAACATTTCAAACCAGTATAAACAGTACTAATCTAGAAGAAGATTTAATTTAAATTAATAAATAACGGCATGCAAATATATATATTGTTTAATTAAGTTTATTTAACGGAGTTGAAGGAAACGATAAAACAGCTAAAAAATATTTTATATTAATATAACTATTTTTCCATAACAAATATATGTCTATGAAAAGATTTTAGACTTCTATAATTGTTGTTATTTATAACTTAGGGATCTAAAAATTAGAATTATCGTGGACAAGAGGTCTCAGATTTAAATCCTCACTCCAACATTTATTACAATACCTTTGTAAAAAAAAATTAAAGTTATTTTATAAGTTTTAATTTTGTTTTCAGTACATTTATGGTTTTGGAAATTAGACCTATTTCATTCTCATTTTGTACAGTATTTTTCTTAAGTGTTGATTTCTGAGACAAAGTATGAAAAGAAAAACAAGTTTTTAAAAGCTACTGTTTTTTTGTTTTCTAATTTTTGTTTGAATTTTTCAATGATTGGTAAAAAGTAAATAACATATCAACAAGACATTTGAAGGTGAAATAAGTGTCTATACAGTTAGTTTTTAAAAATAGAAATAAAAAATAAAATGATTACCAAATGAGCCTTACTTTTTTGTTTTTAAAATTAATTAAGCCTATAATAAAACATTAATTCTACATCTAAATTTCTTTATTTTTTACTGTTTACTAAATACCATTTAATTATTTGAAAATCCAAGGAAAATTTTGAAGACTGAAAAAAAGTAGTTTAAAAAATAAACAATTTTTGTAAGGCTCTTGATTGCATACCGCCAACGCGATATTGACCCTGCGCCAAGGATGTTGAGAGAAGAGATTTATAATAGTAGATCAAATATATATATTCCGTGAAAGGAGTATGCATGAGACTCTAACCCGAAGAAAGTAATTCCAAATGGAGAGGATCCTCGTTGAAAAGTTATTTTGGATAGAAGTATAGGATATTCTGCGAAGGAGCGTGAAAAGAGAAGGAGACGACACGAAGAGAAATGCTTGTCTAAGTCATATCGTGTGAGTGTAAGCGATGTAATAAAAGTAAGTAGCAAGGAATAGGTCTACGTTGAATGTTAAAAGTTAAAGGTTTAATAGTGTAGAAAGAACTACCCATGCCTACTAGAGTGCGACAAGAAGTAGGTGCCCTGTTGACGTGACTGGACGATCCCATAAAGTATGGATCTGATGTTTCCCAAGAAGATAAGGGAGAGTCTGACGATTAAAGAGTTTGACTCCGCAAGGAGATAAGGTATACATGTCTCAAGGAAAAGTCAAATCGCTGCTAATATTCAGACTATGTGTTGAGTTTGGGTTGACTGCATGACGCGGCAAAATGATGAGGAAATATGTGTAAAGCGTAAAAGCTTCTTTAGTGAAAAAGCCAAAGGAAGAGGAGAGTCTTTGGTGGAAAAAGATAAGACTTTCTGATGAAAGGGACAAGAAGAAGTTCGAAGTGCTGAACGACAACATGAGAGAATCGCTTTGGGGAAGAGGAACCTAGAAGTAGAGAAGGAAGGAATTGAGGTTAAGTATATGTGTCTTTGTTAGGCATTGAGGCCACTCAAAGATTAGTTCTGAGTGACTCTGTAACCAAGCTAAGGAGGTAAGACAGAATGTTTCAATAGTGTTACATGGTGCGTTGGCTATGAAAGTTTCTATTACGCACTTAACGAAGCCGATTGGTAAGCGAATCGCTCCTTAGCACAAATGGCTCAGCTTAAAAAGTAGGTGAGTGTAATACCCGGAATGGCAAGGTCAAGCTAGAGGATTAAAGATTTAAGTGAGAAAAAAAGTAACCCCAAACACGGATCGCTAAGAGAGGCAGTTAGGCGATTGAGGTGAAGAACTAAGGTGTGTTAAGAAAGCCTGCCATTAAACCAATGAGAGTAAAAGTTGTTGTACTCCGTTGCGTTGTGTTAAGAAAACCTGACATTAAGCCTTCCGTTTAAGTATTCGTTGAAAAGTTGTTTCGCTTACTAAGCAGTCAGCGTTATCAGGAGGTGTGATATACATTTTGGGCTTAAGCGACATGCCTCCCCTTAGTCACAGAGAGCGACTTTGCGGTGGGGCATGATAATTTTTTTTTCGAAATTTAGTACATAATACAAAACTCTTGTGCTAATGTAAAATGCACATCATTTTAATCTAGAGACATAAATAGTTAGTACAAGAGACCTAAATAAATTATCAAGTGTAGTCTTTGTTGGAAAGAAACTTTTCATGTTGGAGGAAATTTAAAAGAGAGAATTGAGAAAAGTGGCTACTTGTGTTATTAGATACGTTTAATTTTTGGCTTGACACAAGTGCTATAACATCCCTCCTATTTGTACTAGTTGATACTACTAAATATATGGCTATAATTTTGCATACACATTTTTAATCTAATGGGTACACATGTCTCTCTAGAATATTATTTATAATTTCTAGATATATTTCTTCAATTTTCTCTACATGTCTCCTACAATTCATTTTTCTAGAAATATTATATTAATACTTCTAGAAATTTGATTTTGATTGGAAAATCAAGTTTATTTTTCTAACGGTTTAAAATTATTATCAACTTAGGGCTGTACCGATGTAGCTTGGGTGACATTGTTCGGGTGACAGGATTCACAAACAAAGCCCCAAAAATTAGTTTTGTACGAAGGAAAAATGTGGTTCTAAACCTTGAGTATGAGAAAACAAATGAAACGGATCTTCGTATGGGTGTAGAAAATGCTGGTGGTGTACTGAAGCCATTTGGGGCAACAATTGTGGACTACACAAGCTATGCTGATACCTCAACAATTCCTGGACACTATGTGTTGTACTGGGAGTTATTAATTGATGGTAATGACCACAACACTCAAACCAATCATTTCATTCCATCTTCAGTGTTTAACGATTGTTGCTTTGCCATCGAAGAATCATTTACTACATTCTACCGTATAAAACGATCGCATGAAAAGACTATCAACCCATTGGAGATTAGAATCGTAAAGAGTGGGACGTTTGAGAAGCTTATGAAGCTGGCCATCAATGGAGGTGCTTCAATGAATCAATACAAGACACCTCGATCTCTAAATTCTAACCAGATTCATTTCATCCAGCTTTTGGAGTCAAACGTTGTTTGCAGTTATTTTAGTCAGAAAAACCCCAGAGTTGGATTACGTTAGTCACCACCGTGCTCGTTTTTCTTTCCCCTACTGCACAGTATTTGTACTTTTTCACCACCGTGCTCGTTGCTCTGCTAGTTTTAACTTTGATGCTTTTCCTTTTCCATC

mRNA sequence

TGTAACCCGAATCTTAAAAATAATCTACAAAGATGCCGTCACATGTTGAAGAGAGTTGGTTGGATGAGAGAGATAAGGAAGTACTACAACACATTCAGCATATCACAAGCAATGCTGGCGAAATCCAACGACAAATTCTAAGTGAAATTCTGTCAACGAATGCTAATGTTGAATATTTACAACAACATGGCCTTCATGCCTCTACTGGTTCTTCAACCTTCAAAAAACTTATACCTCTTGTCTCTTACGAACAATTAAAACCTTATATAACTCGTATTGCAGAAGGTGATGATTCCCCAATCCTATGCTCAAACCCCATTACAGCGTTCTTTCTAAGCTCTGGGACATCTGGGGGAGAACCCAAGTTAGTTCCAATATATGAAAAAGAGTTTGAGAGAAGGTTGTCTTTTTTCAACTATTTGATGGCAAGGACGAAAGAATTGTTTCCAAATATCAATTGGCACAAAGGTAAAGCTATGAACTTTCACTTTGCAAAGCCTGATCATAAGACAAAAGCTGGAATTCTTGTTCATACTGTATTTTCAAGACTGCTCAAAAGATCACTAAATCTCAAAAGTGTAGAAAGTGGGAATAACGCAATCCCTGATGACATTCTTCGATGCACGAACACATACCAAAGCTTATACTGTCAGCTTCTTTGTGGGCTCTACCAAAACGATCTAGTTTTTCAAGTTGGTGCAGTTTTGGCTTCTGGGTTGATTCACGTTTTTAAGTTCCTTGAAAATCATTGGGTTGATTTGGTTAGTGATATACGTAGAGGAAGTATTAATAACCCCAAAATTACAGACTTATCACTGAGAGAATCAGTTATGAAGATTCTTGTAAAACCCAATCCTCAACTTGCAGATTTGATTGAAACTGAATGTAGCAAAGGAAAATGGAAAGGAATTGTTCCAAAATTGTGGCCAAACACTAAATATATTAAGGCTATTGCCACAGGAAGCTTGTCACAATACATTCCATTGCTAAATTACTACACAAACAACCTCCCTATATTTTCTGACCATTATGGTTCTACTGAATGTTTCCTTGGCTTGAATCTGGATCCAATATGTGACCCTAATGAAACCTCCTACACTTTGATCCCTACCATGGCGTATTTCGAGTTCTTACCAATAGATACCACAAATATCAATGGCGAAGTGACCCAAGAATTAGTTGATCTTGTTGATGTCAAGTTAGGACAAGAATATGAGCTCGTCATCACCACTTTCGCTGGGCTGTACCGATGTAGCTTGGGTGACATTGTTCGGGTGACAGGATTCACAAACAAAGCCCCAAAAATTAGTTTTGTACGAAGGAAAAATGTGGTTCTAAACCTTGAGTATGAGAAAACAAATGAAACGGATCTTCGTATGGGTGTAGAAAATGCTGGTGGTGTACTGAAGCCATTTGGGGCAACAATTGTGGACTACACAAGCTATGCTGATACCTCAACAATTCCTGGACACTATGTGTTGTACTGGGAGTTATTAATTGATGGTAATGACCACAACACTCAAACCAATCATTTCATTCCATCTTCAGTGTTTAACGATTGTTGCTTTGCCATCGAAGAATCATTTACTACATTCTACCGTATAAAACGATCGCATGAAAAGACTATCAACCCATTGGAGATTAGAATCGTAAAGAGTGGGACGTTTGAGAAGCTTATGAAGCTGGCCATCAATGGAGGTGCTTCAATGAATCAATACAAGACACCTCGATCTCTAAATTCTAACCAGATTCATTTCATCCAGCTTTTGGAGTCAAACGTTGTTTGCAGTTATTTTAGTCAGAAAAACCCCAGAGTTGGATTACGTTAGTCACCACCGTGCTCGTTTTTCTTTCCCCTACTGCACAGTATTTGTACTTTTTCACCACCGTGCTCGTTGCTCTGCTAGTTTTAACTTTGATGCTTTTCCTTTTCCATC

Coding sequence (CDS)

ATGCCGTCACATGTTGAAGAGAGTTGGTTGGATGAGAGAGATAAGGAAGTACTACAACACATTCAGCATATCACAAGCAATGCTGGCGAAATCCAACGACAAATTCTAAGTGAAATTCTGTCAACGAATGCTAATGTTGAATATTTACAACAACATGGCCTTCATGCCTCTACTGGTTCTTCAACCTTCAAAAAACTTATACCTCTTGTCTCTTACGAACAATTAAAACCTTATATAACTCGTATTGCAGAAGGTGATGATTCCCCAATCCTATGCTCAAACCCCATTACAGCGTTCTTTCTAAGCTCTGGGACATCTGGGGGAGAACCCAAGTTAGTTCCAATATATGAAAAAGAGTTTGAGAGAAGGTTGTCTTTTTTCAACTATTTGATGGCAAGGACGAAAGAATTGTTTCCAAATATCAATTGGCACAAAGGTAAAGCTATGAACTTTCACTTTGCAAAGCCTGATCATAAGACAAAAGCTGGAATTCTTGTTCATACTGTATTTTCAAGACTGCTCAAAAGATCACTAAATCTCAAAAGTGTAGAAAGTGGGAATAACGCAATCCCTGATGACATTCTTCGATGCACGAACACATACCAAAGCTTATACTGTCAGCTTCTTTGTGGGCTCTACCAAAACGATCTAGTTTTTCAAGTTGGTGCAGTTTTGGCTTCTGGGTTGATTCACGTTTTTAAGTTCCTTGAAAATCATTGGGTTGATTTGGTTAGTGATATACGTAGAGGAAGTATTAATAACCCCAAAATTACAGACTTATCACTGAGAGAATCAGTTATGAAGATTCTTGTAAAACCCAATCCTCAACTTGCAGATTTGATTGAAACTGAATGTAGCAAAGGAAAATGGAAAGGAATTGTTCCAAAATTGTGGCCAAACACTAAATATATTAAGGCTATTGCCACAGGAAGCTTGTCACAATACATTCCATTGCTAAATTACTACACAAACAACCTCCCTATATTTTCTGACCATTATGGTTCTACTGAATGTTTCCTTGGCTTGAATCTGGATCCAATATGTGACCCTAATGAAACCTCCTACACTTTGATCCCTACCATGGCGTATTTCGAGTTCTTACCAATAGATACCACAAATATCAATGGCGAAGTGACCCAAGAATTAGTTGATCTTGTTGATGTCAAGTTAGGACAAGAATATGAGCTCGTCATCACCACTTTCGCTGGGCTGTACCGATGTAGCTTGGGTGACATTGTTCGGGTGACAGGATTCACAAACAAAGCCCCAAAAATTAGTTTTGTACGAAGGAAAAATGTGGTTCTAAACCTTGAGTATGAGAAAACAAATGAAACGGATCTTCGTATGGGTGTAGAAAATGCTGGTGGTGTACTGAAGCCATTTGGGGCAACAATTGTGGACTACACAAGCTATGCTGATACCTCAACAATTCCTGGACACTATGTGTTGTACTGGGAGTTATTAATTGATGGTAATGACCACAACACTCAAACCAATCATTTCATTCCATCTTCAGTGTTTAACGATTGTTGCTTTGCCATCGAAGAATCATTTACTACATTCTACCGTATAAAACGATCGCATGAAAAGACTATCAACCCATTGGAGATTAGAATCGTAAAGAGTGGGACGTTTGAGAAGCTTATGAAGCTGGCCATCAATGGAGGTGCTTCAATGAATCAATACAAGACACCTCGATCTCTAAATTCTAACCAGATTCATTTCATCCAGCTTTTGGAGTCAAACGTTGTTTGCAGTTATTTTAGTCAGAAAAACCCCAGAGTTGGATTACGTTAG

Protein sequence

MPSHVEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSSTFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNLKSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTNKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGHYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIVKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGLR*
Homology
[Close]
BLAST of CSPI02G07750 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 7.6e-173
Identity = 312/598 (52.17%), Postives = 416/598 (69.57%), Query Frame = 0 

Query: 10  LDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSSTFKKLIPL 69
           LD+++K+ LQ I+ +TSNA ++QRQ+L EIL+ NA+VEYL++H L+  T   TFK ++P+
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 70  VSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRLSFFNY 129
           ++YE ++P I RIA GD SPIL S PI+ F  SSGTSGGE KL+P  E+E +RR   ++ 
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 130 LMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNLKSVES--GN 189
           LM    +  P +    GK M F F K + KT  G+    V +   K S   +       N
Sbjct: 137 LMPVMSQFVPGL--ENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTN 196

Query: 190 NAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVDLVSDI 249
              P++ + C+++YQS+Y Q+LCGL Q+  V +VGAV ASG I   KFLE HW++LV DI
Sbjct: 197 YTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDI 256

Query: 250 RRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTKYIKAI 309
           R G++++  ITD S+RE+V KIL KP+P+LAD +E EC K  W+GI+ +LWPNTKY+  I
Sbjct: 257 RTGTLSS-LITDPSVREAVAKIL-KPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVI 316

Query: 310 ATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMAYFEFL 369
            TG++SQYIP L+YY+N LP+    Y S+EC+ G+NL P+C P+E SYTLIP+MAYFEFL
Sbjct: 317 VTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFL 376

Query: 370 PIDTTN-----------INGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVT 429
           P+   N           +  +  QELVDLVDVKLGQEYELV+TT+AGL R  +GD++RVT
Sbjct: 377 PVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVT 436

Query: 430 GFTNKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTST 489
           GF NKAP+ SF+ RKNVVL+++ +KT+E +L+  V+NA   L PF A++ +YTSYADTS+
Sbjct: 437 GFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSS 496

Query: 490 IPGHYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLE 549
           IPGHYVL+WEL +DGN         IP SVF DCC A+EESF T YR  R  +K+I PLE
Sbjct: 497 IPGHYVLFWELCLDGNTP-------IPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLE 556

Query: 550 IRIVKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPR 595
           I+IV+ GTF+KLM  AI+ GAS+NQYKTPR +    I  I+LL S VV SYFS K P+
Sbjct: 557 IKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPI--IELLNSRVVDSYFSPKCPK 601

[Close]
BLAST of CSPI02G07750 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 6.0e-170
Identity = 308/603 (51.08%), Postives = 414/603 (68.66%), Query Frame = 0 

Query: 5   VEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSSTFK 64
           V +  L E++K  LQ I+ +T+NA ++QR++L EILS NA+VEYL++HGL   T   TFK
Sbjct: 12  VSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFK 71

Query: 65  KLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRL 124
            ++P+V+YE ++P I RIA GD S +LCSNPI+ F  SSGTSGGE KL+P  E+E +RR 
Sbjct: 72  HIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRS 131

Query: 125 SFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRS--LNLKS 184
             ++ LM    +  P ++  KGK M F F K + KT  G+    V +   K S   N   
Sbjct: 132 LLYSLLMPVMDQFVPGLD--KGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPY 191

Query: 185 VESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVD 244
               N   P+  + C+++YQS+Y Q+LCGL Q+  V +VGAV ASG I   KFLE HW +
Sbjct: 192 DPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPE 251

Query: 245 LVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTK 304
           L  DIR G++++ +ITD S+RE+V +IL KP+P+LAD +E+EC K  W+GI+ +LWPNTK
Sbjct: 252 LARDIRTGTLSS-EITDSSVREAVGEIL-KPDPKLADFVESECRKTSWQGIITRLWPNTK 311

Query: 305 YIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMA 364
           Y+  I TG++SQYIP L+YY+N LP+    Y S+EC+ G+NL P+C P+E SYTLIP MA
Sbjct: 312 YVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMA 371

Query: 365 YFEFLPIDTTN-----------INGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGD 424
           YFEFLP+   +           +  +  QELVDLVDVKLGQEYELV+TT+AGLYR  +GD
Sbjct: 372 YFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGD 431

Query: 425 IVRVTGFTNKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSY 484
           ++ V GF N AP+ SF+ RKNVVL+++ +KT+E +L+  V+NA   L PF A++ +YTSY
Sbjct: 432 VLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSY 491

Query: 485 ADTSTIPGHYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKT 544
           ADTS+IPGHYVL+WEL ++GN         IP SVF DCC  IEES  + YR  R  +K+
Sbjct: 492 ADTSSIPGHYVLFWELCLNGNTP-------IPPSVFEDCCLTIEESLNSVYRQGRVSDKS 551

Query: 545 INPLEIRIVKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQK 595
           I PLEI++V+SGTF+KLM  AI+ GAS+NQYKTPR +    I  I+LL S VV SYFS K
Sbjct: 552 IGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPI--IELLNSRVVDSYFSPK 601

[Close]
BLAST of CSPI02G07750 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 1.8e-153
Identity = 274/583 (47.00%), Postives = 386/583 (66.21%), Query Frame = 0 

Query: 16  EVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTG-SSTFKKLIPLVSYEQ 75
           E L+ I+H+T+NAG++QR++L EIL+ NA  EYL+++G+  S      F++L+PLV+YE 
Sbjct: 22  EALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEG 81

Query: 76  LKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRLSFFNYLMART 135
           L+P I RIA GD SPI    PI+ F  SSGTSGGE KL+P    E  RR   ++ LM   
Sbjct: 82  LQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVM 141

Query: 136 KELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLL--KRSLNLKSVESGNNAIPD 195
            +    ++  KGKAM   F K + +T  G+    V +     ++ L+       +   PD
Sbjct: 142 SQSVSGLD--KGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPD 201

Query: 196 DILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVDLVSDIRRGSI 255
           + + C ++YQS+Y QLLCGL     V +VGAV ASG +    FLE HW  L  DIR G +
Sbjct: 202 EAILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGEL 261

Query: 256 NNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTKYIKAIATGSL 315
            +P+ITD  +R++V ++L + +P LAD IE EC++  W+GI+ +LWP TKYI  I TG++
Sbjct: 262 -DPEITDRVVRDAVGRVL-RADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTM 321

Query: 316 SQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMAYFEFLPIDTT 375
           SQYIP L +Y   LP+    Y S+EC+ GLNL+P+C P++ +YTLIPTM Y+EFLP++  
Sbjct: 322 SQYIPTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCN 381

Query: 376 NINGEVT-QELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTNKAPKISFVRRK 435
           N   E + ++LVDLVDVKLG EYELV+TT++GLYR  +GD++RV GF NKAP  SFVRR+
Sbjct: 382 NATAEASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVRRQ 441

Query: 436 NVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGHYVLYWELLIDG 495
           NV L+++ +KT+ET+L   V  A   L PFGA++V+YTSYAD +TIPGHYVL+WEL    
Sbjct: 442 NVALSVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWELRAGS 501

Query: 496 NDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIVKSGTFEKLMKL 555
                     +P+SVF +CC ++EE+  + YR  R+ +++I PLEIR+V  GTF+KLM  
Sbjct: 502 T--------AVPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDY 561

Query: 556 AINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPR 595
           AI+ GAS+NQYK PR +    +  ++LL++ V   YFS K P+
Sbjct: 562 AISRGASINQYKAPRCVRPGPV--VELLDARVQGKYFSPKCPK 590

[Close]
BLAST of CSPI02G07750 vs. ExPASy Swiss-Prot
Match: Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 9.0e-150
Identity = 272/590 (46.10%), Postives = 380/590 (64.41%), Query Frame = 0 

Query: 15  KEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGL--HASTGSSTFKKLIPLVSY 74
           +E L+HI+ +T NAG+ QR++L EIL+ NA  EYL++ G+   A      F++L PLV+Y
Sbjct: 24  REKLEHIERVTRNAGQEQRRVLEEILAQNAQAEYLRRLGVPGDAPGADEAFRRLAPLVTY 83

Query: 75  EQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRLSFFNYLMA 134
           E + P + RIA GD SPIL   P++ F  SSGTSGGE KL+P  E+E ERR   ++ LM 
Sbjct: 84  EDILPDVLRIANGDTSPILSGKPVSEFLTSSGTSGGERKLMPTIEEEMERRSGLYSLLMP 143

Query: 135 RTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNLKSVESGNNAI-- 194
                 P ++  KGKAM  +F K + +T  G+    V +   +    L+           
Sbjct: 144 VMSRQVPGLD--KGKAMYLYFVKSEWRTPGGLPARPVLTSFYRSRYFLERPHDPYTVYTS 203

Query: 195 PDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVDLVSDIRRG 254
           PD+ + C + YQS+Y QL+CGL     V +VGAV ASG +   +FLE HW  L  DIR G
Sbjct: 204 PDEAVLCEDAYQSMYAQLICGLVHRADVLRVGAVFASGFLRAIRFLEKHWPSLCRDIRAG 263

Query: 255 SINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTKYIKAIATG 314
            ++   +TD ++R +V ++L   +P LAD IE EC++  W+GI+ ++WP+TKYI  I TG
Sbjct: 264 ELDG-GVTDPAVRGAVGRVLRGADPALADAIEAECARPSWQGIIRRVWPSTKYIDVIVTG 323

Query: 315 SLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMAYFEFLPID 374
           +++QYIP L +Y   LP+    Y S+EC+ GLNL+P+C P+E +YTLIPTM YFEFLP++
Sbjct: 324 AMAQYIPTLEFYGGGLPLACTMYASSECYFGLNLNPMCKPSEVAYTLIPTMCYFEFLPVN 383

Query: 375 TTNINGEVTQE-----LVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTNKAPKI 434
            +  N     E     LVDLVDVKLG EYELV+TT++GLYR  +GD++RV GF N AP  
Sbjct: 384 -SGANDVAAPEPDHRGLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNAAPMF 443

Query: 435 SFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADT-STIPGHYVLY 494
           +FVRRKNV L+++ +KT+E +L   V  A   L PFGA++V+YTSYADT +TIPGHYVL+
Sbjct: 444 AFVRRKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASLVEYTSYADTATTIPGHYVLF 503

Query: 495 WELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIVKSGT 554
           WEL       +      +P+SVF DCC A+EE   + YR  R+ +++I PLEIR+V  GT
Sbjct: 504 WEL------RSPAGGTPVPASVFEDCCLAVEEGLNSVYRQCRAADRSIGPLEIRVVADGT 563

Query: 555 FEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPR 595
           F+KLM  A++ GAS+NQYK PR +    +  ++LL+  V  +YFS K P+
Sbjct: 564 FDKLMDYALSRGASINQYKAPRCVRPGPV--VELLDGRVQATYFSPKCPK 601

[Close]
BLAST of CSPI02G07750 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 525.0 bits (1351), Expect = 1.1e-147
Identity = 271/585 (46.32%), Postives = 376/585 (64.27%), Query Frame = 0 

Query: 12  ERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSSTFKKLIPLVS 71
           E D E L+ I  +T+N   +Q ++L EIL  NA  EYL + GL  +T  + F+  +P+VS
Sbjct: 24  EGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVS 83

Query: 72  YEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRLSFFNYLM 131
           Y+ L+PYI RIA GD SPIL ++P++ F  SSGTS GE KL+P    E +RR   ++ LM
Sbjct: 84  YDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLM 143

Query: 132 ARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRS--LNLKSVESGNNA 191
                  P ++  KGK + F F K + KT  G+    V +   K     N       N  
Sbjct: 144 PVMNLYVPGLD--KGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYT 203

Query: 192 IPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVDLVSDIRR 251
            P   + C + +QS+Y Q++CGL Q + V ++GAV ASGL+   +FL+ +W  L  DI  
Sbjct: 204 SPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIES 263

Query: 252 GSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTKYIKAIAT 311
           G +  P++TD S+RE+V  IL+ P+P+LA LI  ECSKG W GI+ ++WPNTKY+  I T
Sbjct: 264 GEL-TPRVTDPSVREAVAAILL-PDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVT 323

Query: 312 GSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMAYFEFLPI 371
           G+++QYIP L +Y+  LP+    Y S+EC+ GLNL P+CDP+E SYT++P M YFEFLP+
Sbjct: 324 GAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPV 383

Query: 372 DTTN-INGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTNKAPKISFV 431
           D T   +G+ TQ LVDL  V++G+EYELVITT+AGL R  +GD++RVTGF N AP+  FV
Sbjct: 384 DETGAASGDATQ-LVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFV 443

Query: 432 RRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGHYVLYWELL 491
           RRKNV+L++E +KT+E +L+  VE A  +L+P GA++V+YTS A T  IPGHYV+YWELL
Sbjct: 444 RRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELL 503

Query: 492 IDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIVKSGTFEKL 551
             G          + +     CC  +EE+  T YR  R  + +I PLEIR+V+ GTFE+L
Sbjct: 504 TKGAGATV-----VDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEEL 563

Query: 552 MKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNP 594
           M  AI+ GAS+NQYK PR +    I  ++LL+S VV S+FS   P
Sbjct: 564 MDYAISRGASINQYKVPRCVTFPPI--VELLDSRVVSSHFSPALP 596

[Close]
BLAST of CSPI02G07750 vs. ExPASy TrEMBL
Match: A0A0A0LHA0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G081190 PE=3 SV=1)

HSP 1 Score: 1216.1 bits (3145), Expect = 0.0e+00
Identity = 595/597 (99.66%), Postives = 595/597 (99.66%), Query Frame = 0 

Query: 1   MPSHVEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS 60
           MPSHVEESWLDERDKE LQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS
Sbjct: 1   MPSHVEESWLDERDKEALQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS 60

Query: 61  STFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEF 120
           STFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEF
Sbjct: 61  STFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEF 120

Query: 121 ERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNL 180
           ERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNL
Sbjct: 121 ERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNL 180

Query: 181 KSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHW 240
           KSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHW
Sbjct: 181 KSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHW 240

Query: 241 VDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPN 300
           VDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPN
Sbjct: 241 VDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPN 300

Query: 301 TKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPT 360
           TKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPT
Sbjct: 301 TKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPT 360

Query: 361 MAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTN 420
           MAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTN
Sbjct: 361 MAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTN 420

Query: 421 KAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGH 480
           KAPK SFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGH
Sbjct: 421 KAPKFSFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGH 480

Query: 481 YVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIV 540
           YVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIV
Sbjct: 481 YVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIV 540

Query: 541 KSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL 598
           KSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL
Sbjct: 541 KSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL 597

[Close]
BLAST of CSPI02G07750 vs. ExPASy TrEMBL
Match: A0A5D3BD93 (Indole-3-acetic acid-amido synthetase GH3.5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold976G00010 PE=3 SV=1)

HSP 1 Score: 1086.6 bits (2809), Expect = 0.0e+00
Identity = 531/598 (88.80%), Postives = 561/598 (93.81%), Query Frame = 0 

Query: 1   MPSHVEESW-LDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTG 60
           M S+VE  W LDE+D E LQ IQ ITSNA EIQ  IL+EILSTNANVEYLQQHGL  ST 
Sbjct: 1   MSSNVEHKWLLDEKDYEALQQIQDITSNADEIQSLILTEILSTNANVEYLQQHGLDGSTD 60

Query: 61  SSTFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKE 120
           SSTFKKLIPLVSYEQL+PYITRIAEGDDSPILCSNPIT FF+SSGTSGGEPKLVPIYE+E
Sbjct: 61  SSTFKKLIPLVSYEQLQPYITRIAEGDDSPILCSNPITGFFVSSGTSGGEPKLVPIYEQE 120

Query: 121 FERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLN 180
           FERRLSFFNYLMARTK+LFPNIN +  KAMNFHFAKPDHKTKAGILV +VF+RLLK SLN
Sbjct: 121 FERRLSFFNYLMARTKQLFPNINCYNDKAMNFHFAKPDHKTKAGILVSSVFTRLLKTSLN 180

Query: 181 LKSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENH 240
           LKS+ +GNNAIPDDIL CT+TYQSLYCQLLCGLY+NDLVFQVG++ ASGL+HVFKFLENH
Sbjct: 181 LKSIATGNNAIPDDILLCTDTYQSLYCQLLCGLYRNDLVFQVGSIFASGLLHVFKFLENH 240

Query: 241 WVDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWP 300
           W DLVSDIR GSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKG WKGIV +LWP
Sbjct: 241 WADLVSDIRTGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGTWKGIVRRLWP 300

Query: 301 NTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIP 360
           NTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTEC LGLNLDPICDPNETSYTLIP
Sbjct: 301 NTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECLLGLNLDPICDPNETSYTLIP 360

Query: 361 TMAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFT 420
           TMAYFEFLP+DTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYR  +GDI+RVTGFT
Sbjct: 361 TMAYFEFLPMDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRYCIGDILRVTGFT 420

Query: 421 NKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPG 480
           NKAPK SFVRRKNV+LNLEYEKTNE+DLR+GVE AGGVLKPFGATIVDYT YADTSTIPG
Sbjct: 421 NKAPKFSFVRRKNVILNLEYEKTNESDLRLGVEKAGGVLKPFGATIVDYTCYADTSTIPG 480

Query: 481 HYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRI 540
           HYVLYWELLIDGNDHNTQTNHFIPSSV+NDCC AIE+S +TFYRIKRS EKTINPLEIR+
Sbjct: 481 HYVLYWELLIDGNDHNTQTNHFIPSSVYNDCCLAIEDSLSTFYRIKRSQEKTINPLEIRV 540

Query: 541 VKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL 598
           VKSGTFEKLM+LAINGGASMNQYKTPRSLNSNQIHFIQLLESNV+ SYFSQKNPRVGL
Sbjct: 541 VKSGTFEKLMQLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVISSYFSQKNPRVGL 598

[Close]
BLAST of CSPI02G07750 vs. ExPASy TrEMBL
Match: A0A1S3B3T7 (indole-3-acetic acid-amido synthetase GH3.5-like OS=Cucumis melo OX=3656 GN=LOC103485860 PE=3 SV=1)

HSP 1 Score: 1086.6 bits (2809), Expect = 0.0e+00
Identity = 531/598 (88.80%), Postives = 561/598 (93.81%), Query Frame = 0 

Query: 1   MPSHVEESW-LDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTG 60
           M S+VE  W LDE+D E LQ IQ ITSNA EIQ  IL+EILSTNANVEYLQQHGL  ST 
Sbjct: 1   MSSNVEHKWLLDEKDYEALQQIQDITSNADEIQSLILTEILSTNANVEYLQQHGLDGSTD 60

Query: 61  SSTFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKE 120
           SSTFKKLIPLVSYEQL+PYITRIAEGDDSPILCSNPIT FF+SSGTSGGEPKLVPIYE+E
Sbjct: 61  SSTFKKLIPLVSYEQLQPYITRIAEGDDSPILCSNPITGFFVSSGTSGGEPKLVPIYEQE 120

Query: 121 FERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLN 180
           FERRLSFFNYLMARTK+LFPNIN +  KAMNFHFAKPDHKTKAGILV +VF+RLLK SLN
Sbjct: 121 FERRLSFFNYLMARTKQLFPNINCYNDKAMNFHFAKPDHKTKAGILVSSVFTRLLKTSLN 180

Query: 181 LKSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENH 240
           LKS+ +GNNAIPDDIL CT+TYQSLYCQLLCGLY+NDLVFQVG++ ASGL+HVFKFLENH
Sbjct: 181 LKSIATGNNAIPDDILLCTDTYQSLYCQLLCGLYRNDLVFQVGSIFASGLLHVFKFLENH 240

Query: 241 WVDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWP 300
           W DLVSDIR GSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKG WKGIV +LWP
Sbjct: 241 WADLVSDIRTGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGTWKGIVRRLWP 300

Query: 301 NTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIP 360
           NTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTEC LGLNLDPICDPNETSYTLIP
Sbjct: 301 NTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECLLGLNLDPICDPNETSYTLIP 360

Query: 361 TMAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFT 420
           TMAYFEFLP+DTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYR  +GDI+RVTGFT
Sbjct: 361 TMAYFEFLPMDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRYCIGDILRVTGFT 420

Query: 421 NKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPG 480
           NKAPK SFVRRKNV+LNLEYEKTNE+DLR+GVE AGGVLKPFGATIVDYT YADTSTIPG
Sbjct: 421 NKAPKFSFVRRKNVILNLEYEKTNESDLRLGVEKAGGVLKPFGATIVDYTCYADTSTIPG 480

Query: 481 HYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRI 540
           HYVLYWELLIDGNDHNTQTNHFIPSSV+NDCC AIE+S +TFYRIKRS EKTINPLEIR+
Sbjct: 481 HYVLYWELLIDGNDHNTQTNHFIPSSVYNDCCLAIEDSLSTFYRIKRSQEKTINPLEIRV 540

Query: 541 VKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL 598
           VKSGTFEKLM+LAINGGASMNQYKTPRSLNSNQIHFIQLLESNV+ SYFSQKNPRVGL
Sbjct: 541 VKSGTFEKLMQLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVISSYFSQKNPRVGL 598

[Close]
BLAST of CSPI02G07750 vs. ExPASy TrEMBL
Match: A0A5A7UAR7 (Indole-3-acetic acid-amido synthetase GH3.5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold55G00030 PE=3 SV=1)

HSP 1 Score: 1084.7 bits (2804), Expect = 0.0e+00
Identity = 530/598 (88.63%), Postives = 560/598 (93.65%), Query Frame = 0 

Query: 1   MPSHVEESW-LDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTG 60
           M S+VE  W LDE+D E LQ IQ ITSNA EIQ  IL+EILSTNANVEYLQQHGL  ST 
Sbjct: 1   MSSNVEHKWLLDEKDYEALQQIQDITSNADEIQSLILTEILSTNANVEYLQQHGLDGSTD 60

Query: 61  SSTFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKE 120
           SSTFKKLIPLVSYEQL+PYITRIAEGDDSPILCSNPIT FF+SSGTSGGEPKLVPIYE+E
Sbjct: 61  SSTFKKLIPLVSYEQLQPYITRIAEGDDSPILCSNPITGFFVSSGTSGGEPKLVPIYEQE 120

Query: 121 FERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLN 180
           FERRLSFFNYLMARTK+LFPNIN +  KAMNFHFAKPDHKTKAGILV +VF+RLLK SLN
Sbjct: 121 FERRLSFFNYLMARTKQLFPNINCYNDKAMNFHFAKPDHKTKAGILVSSVFTRLLKTSLN 180

Query: 181 LKSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENH 240
           LKS+ +GNNAIPDDIL CT+TYQSLYCQLLCGLYQND VFQVG++ ASGL+HVFKFLENH
Sbjct: 181 LKSIATGNNAIPDDILLCTDTYQSLYCQLLCGLYQNDPVFQVGSIFASGLLHVFKFLENH 240

Query: 241 WVDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWP 300
           W DLVSDIR GSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKG WKGIV +LWP
Sbjct: 241 WADLVSDIRTGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGTWKGIVRRLWP 300

Query: 301 NTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIP 360
           NTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTEC LGLNLDPICDPNETSYTLIP
Sbjct: 301 NTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECLLGLNLDPICDPNETSYTLIP 360

Query: 361 TMAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFT 420
           TMAYFEFLP+DTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYR  +GDI+RVTGFT
Sbjct: 361 TMAYFEFLPMDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRYCIGDILRVTGFT 420

Query: 421 NKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPG 480
           NKAPK SFVRRKNV+LNLEYEKTNE+DLR+GVE AGGVLKPFGATIVDYT YADTSTIPG
Sbjct: 421 NKAPKFSFVRRKNVILNLEYEKTNESDLRLGVEKAGGVLKPFGATIVDYTCYADTSTIPG 480

Query: 481 HYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRI 540
           HYVLYWELLIDGNDHNTQTNHFIPSSV+NDCC AIE+S +TFYRIKRS EKTINPLEIR+
Sbjct: 481 HYVLYWELLIDGNDHNTQTNHFIPSSVYNDCCLAIEDSLSTFYRIKRSQEKTINPLEIRV 540

Query: 541 VKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL 598
           VKSGTFEKLM+LAINGGASMNQYKTPRSLNSNQIHFIQL+ESNV+ SYFSQKNPRVGL
Sbjct: 541 VKSGTFEKLMQLAINGGASMNQYKTPRSLNSNQIHFIQLMESNVISSYFSQKNPRVGL 598

[Close]
BLAST of CSPI02G07750 vs. ExPASy TrEMBL
Match: A0A5A7V3L5 (Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G00190 PE=3 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 2.6e-253
Identity = 427/593 (72.01%), Postives = 494/593 (83.31%), Query Frame = 0 

Query: 3   SHVEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSST 62
           S+ EE WLDE+DKE LQ+I+ IT+ A EIQRQIL+EILS NANVEYLQ+HGL AST SST
Sbjct: 2   SNFEEKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSST 61

Query: 63  FKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFER 122
           FKKLIPLV Y+QL+PYI RIA+GDDS ILCSNPIT FF SSGTSG E KL+P+YE+EF R
Sbjct: 62  FKKLIPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFVR 121

Query: 123 RLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNLKS 182
           RLSFF+Y+  R K+LFPNINWHK K +NFHFAKP+ KTK GI+V ++F+ L KRS NL+S
Sbjct: 122 RLSFFSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLRS 181

Query: 183 VESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVD 242
           + SGNN  PDDI+ CT++YQSLYCQLLCGLYQND+VF+V A+ AS LIHVFKFLENHW+D
Sbjct: 182 MPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWID 241

Query: 243 LVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTK 302
           L +DIR  +I NPKIT  S+RESVMKI+VKPNP++ADLIE EC KG+W+GI+ +LWPN K
Sbjct: 242 LATDIRTRTI-NPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAK 301

Query: 303 YIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMA 362
           YI A+ TGS+SQYIPLLNYYTNNLPI SDHYGS+ECFLGLNLDP+C+P+E SYTLIPTMA
Sbjct: 302 YINAVVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTMA 361

Query: 363 YFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTNKA 422
           YFEFLPID  N  GEV Q LVDLVDVKLG EYELVITTFAGLYR  +GD+VRVTGF NKA
Sbjct: 362 YFEFLPIDMLNPKGEVNQRLVDLVDVKLGHEYELVITTFAGLYRYCMGDVVRVTGFKNKA 421

Query: 423 PKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGHYV 482
           P  SFVRRKNVVLN+  EKT+E  L   VE  G V+K FGATIVDYT+YAD STIPGHYV
Sbjct: 422 PNFSFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATIVDYTTYADISTIPGHYV 481

Query: 483 LYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIVKS 542
           LYWEL +D  D   Q  ++IP SVF DCC AIE S    YR+ RSHEK INPLEI+IVK+
Sbjct: 482 LYWELTLD--DLKEQNLNYIPCSVFEDCCLAIENSLNLLYRLARSHEKCINPLEIKIVKA 541

Query: 543 GTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRV 596
           GTFEKLM+LA+N GAS+ QYKTPR LNS+QIH IQLLESN+V +YFS+  P +
Sbjct: 542 GTFEKLMQLALNRGASITQYKTPRCLNSSQIHIIQLLESNLVSNYFSKNYPNL 591

[Close]
BLAST of CSPI02G07750 vs. NCBI nr
Match: XP_004138974.1 (indole-3-acetic acid-amido synthetase GH3.5 isoform X1 [Cucumis sativus] >KGN61320.1 hypothetical protein Csa_006424 [Cucumis sativus])

HSP 1 Score: 1216.1 bits (3145), Expect = 0.0e+00
Identity = 595/597 (99.66%), Postives = 595/597 (99.66%), Query Frame = 0 

Query: 1   MPSHVEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS 60
           MPSHVEESWLDERDKE LQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS
Sbjct: 1   MPSHVEESWLDERDKEALQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS 60

Query: 61  STFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEF 120
           STFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEF
Sbjct: 61  STFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEF 120

Query: 121 ERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNL 180
           ERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNL
Sbjct: 121 ERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNL 180

Query: 181 KSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHW 240
           KSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHW
Sbjct: 181 KSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHW 240

Query: 241 VDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPN 300
           VDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPN
Sbjct: 241 VDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPN 300

Query: 301 TKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPT 360
           TKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPT
Sbjct: 301 TKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPT 360

Query: 361 MAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTN 420
           MAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTN
Sbjct: 361 MAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTN 420

Query: 421 KAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGH 480
           KAPK SFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGH
Sbjct: 421 KAPKFSFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGH 480

Query: 481 YVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIV 540
           YVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIV
Sbjct: 481 YVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIV 540

Query: 541 KSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL 598
           KSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL
Sbjct: 541 KSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL 597

[Close]
BLAST of CSPI02G07750 vs. NCBI nr
Match: XP_008441807.1 (PREDICTED: indole-3-acetic acid-amido synthetase GH3.5-like [Cucumis melo] >TYJ96956.1 indole-3-acetic acid-amido synthetase GH3.5-like [Cucumis melo var. makuwa])

HSP 1 Score: 1086.6 bits (2809), Expect = 0.0e+00
Identity = 531/598 (88.80%), Postives = 561/598 (93.81%), Query Frame = 0 

Query: 1   MPSHVEESW-LDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTG 60
           M S+VE  W LDE+D E LQ IQ ITSNA EIQ  IL+EILSTNANVEYLQQHGL  ST 
Sbjct: 1   MSSNVEHKWLLDEKDYEALQQIQDITSNADEIQSLILTEILSTNANVEYLQQHGLDGSTD 60

Query: 61  SSTFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKE 120
           SSTFKKLIPLVSYEQL+PYITRIAEGDDSPILCSNPIT FF+SSGTSGGEPKLVPIYE+E
Sbjct: 61  SSTFKKLIPLVSYEQLQPYITRIAEGDDSPILCSNPITGFFVSSGTSGGEPKLVPIYEQE 120

Query: 121 FERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLN 180
           FERRLSFFNYLMARTK+LFPNIN +  KAMNFHFAKPDHKTKAGILV +VF+RLLK SLN
Sbjct: 121 FERRLSFFNYLMARTKQLFPNINCYNDKAMNFHFAKPDHKTKAGILVSSVFTRLLKTSLN 180

Query: 181 LKSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENH 240
           LKS+ +GNNAIPDDIL CT+TYQSLYCQLLCGLY+NDLVFQVG++ ASGL+HVFKFLENH
Sbjct: 181 LKSIATGNNAIPDDILLCTDTYQSLYCQLLCGLYRNDLVFQVGSIFASGLLHVFKFLENH 240

Query: 241 WVDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWP 300
           W DLVSDIR GSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKG WKGIV +LWP
Sbjct: 241 WADLVSDIRTGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGTWKGIVRRLWP 300

Query: 301 NTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIP 360
           NTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTEC LGLNLDPICDPNETSYTLIP
Sbjct: 301 NTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECLLGLNLDPICDPNETSYTLIP 360

Query: 361 TMAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFT 420
           TMAYFEFLP+DTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYR  +GDI+RVTGFT
Sbjct: 361 TMAYFEFLPMDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRYCIGDILRVTGFT 420

Query: 421 NKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPG 480
           NKAPK SFVRRKNV+LNLEYEKTNE+DLR+GVE AGGVLKPFGATIVDYT YADTSTIPG
Sbjct: 421 NKAPKFSFVRRKNVILNLEYEKTNESDLRLGVEKAGGVLKPFGATIVDYTCYADTSTIPG 480

Query: 481 HYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRI 540
           HYVLYWELLIDGNDHNTQTNHFIPSSV+NDCC AIE+S +TFYRIKRS EKTINPLEIR+
Sbjct: 481 HYVLYWELLIDGNDHNTQTNHFIPSSVYNDCCLAIEDSLSTFYRIKRSQEKTINPLEIRV 540

Query: 541 VKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL 598
           VKSGTFEKLM+LAINGGASMNQYKTPRSLNSNQIHFIQLLESNV+ SYFSQKNPRVGL
Sbjct: 541 VKSGTFEKLMQLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVISSYFSQKNPRVGL 598

[Close]
BLAST of CSPI02G07750 vs. NCBI nr
Match: KAA0051296.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Cucumis melo var. makuwa])

HSP 1 Score: 1084.7 bits (2804), Expect = 0.0e+00
Identity = 530/598 (88.63%), Postives = 560/598 (93.65%), Query Frame = 0 

Query: 1   MPSHVEESW-LDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTG 60
           M S+VE  W LDE+D E LQ IQ ITSNA EIQ  IL+EILSTNANVEYLQQHGL  ST 
Sbjct: 1   MSSNVEHKWLLDEKDYEALQQIQDITSNADEIQSLILTEILSTNANVEYLQQHGLDGSTD 60

Query: 61  SSTFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKE 120
           SSTFKKLIPLVSYEQL+PYITRIAEGDDSPILCSNPIT FF+SSGTSGGEPKLVPIYE+E
Sbjct: 61  SSTFKKLIPLVSYEQLQPYITRIAEGDDSPILCSNPITGFFVSSGTSGGEPKLVPIYEQE 120

Query: 121 FERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLN 180
           FERRLSFFNYLMARTK+LFPNIN +  KAMNFHFAKPDHKTKAGILV +VF+RLLK SLN
Sbjct: 121 FERRLSFFNYLMARTKQLFPNINCYNDKAMNFHFAKPDHKTKAGILVSSVFTRLLKTSLN 180

Query: 181 LKSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENH 240
           LKS+ +GNNAIPDDIL CT+TYQSLYCQLLCGLYQND VFQVG++ ASGL+HVFKFLENH
Sbjct: 181 LKSIATGNNAIPDDILLCTDTYQSLYCQLLCGLYQNDPVFQVGSIFASGLLHVFKFLENH 240

Query: 241 WVDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWP 300
           W DLVSDIR GSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKG WKGIV +LWP
Sbjct: 241 WADLVSDIRTGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGTWKGIVRRLWP 300

Query: 301 NTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIP 360
           NTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTEC LGLNLDPICDPNETSYTLIP
Sbjct: 301 NTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECLLGLNLDPICDPNETSYTLIP 360

Query: 361 TMAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFT 420
           TMAYFEFLP+DTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYR  +GDI+RVTGFT
Sbjct: 361 TMAYFEFLPMDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRYCIGDILRVTGFT 420

Query: 421 NKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPG 480
           NKAPK SFVRRKNV+LNLEYEKTNE+DLR+GVE AGGVLKPFGATIVDYT YADTSTIPG
Sbjct: 421 NKAPKFSFVRRKNVILNLEYEKTNESDLRLGVEKAGGVLKPFGATIVDYTCYADTSTIPG 480

Query: 481 HYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRI 540
           HYVLYWELLIDGNDHNTQTNHFIPSSV+NDCC AIE+S +TFYRIKRS EKTINPLEIR+
Sbjct: 481 HYVLYWELLIDGNDHNTQTNHFIPSSVYNDCCLAIEDSLSTFYRIKRSQEKTINPLEIRV 540

Query: 541 VKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL 598
           VKSGTFEKLM+LAINGGASMNQYKTPRSLNSNQIHFIQL+ESNV+ SYFSQKNPRVGL
Sbjct: 541 VKSGTFEKLMQLAINGGASMNQYKTPRSLNSNQIHFIQLMESNVISSYFSQKNPRVGL 598

[Close]
BLAST of CSPI02G07750 vs. NCBI nr
Match: XP_038902067.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida])

HSP 1 Score: 915.2 bits (2364), Expect = 2.9e-262
Identity = 446/594 (75.08%), Postives = 510/594 (85.86%), Query Frame = 0 

Query: 3   SHVEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSST 62
           SHV E WLDE+DKE LQHI+ +TS A EIQRQIL+EILSTNANVEYLQQHGLH ST +ST
Sbjct: 2   SHVVEHWLDEKDKEALQHIEDVTSKADEIQRQILTEILSTNANVEYLQQHGLHGSTDAST 61

Query: 63  FKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFER 122
           FKKLIPL+SYEQL PY+  IA  DDSPILCSNPIT FF SSGTSGGE KL+P+YE+EF R
Sbjct: 62  FKKLIPLISYEQLTPYLALIAHDDDSPILCSNPITHFFTSSGTSGGETKLIPMYEEEFVR 121

Query: 123 RLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNLKS 182
           RLSFFNY+M R K+LFPNINW KGK MNFHFAKP+ KTKAGI+V ++F+ L KRS NLKS
Sbjct: 122 RLSFFNYMMPRMKQLFPNINWQKGKGMNFHFAKPEFKTKAGIIVRSIFTSLHKRSSNLKS 181

Query: 183 VESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVD 242
              GNN  PDDI+ CT+++QSLYCQLLCGLYQND+VF++GA+ AS LIHVFKFLENHWV 
Sbjct: 182 KPIGNNTSPDDIILCTDSFQSLYCQLLCGLYQNDVVFRLGALFASTLIHVFKFLENHWVV 241

Query: 243 LVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTK 302
           L +DI  GSI NPKIT+LS+RESVMKI+VKPNP++ADLIE ECSKG W+GI+ +LWPNTK
Sbjct: 242 LANDILTGSI-NPKITNLSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTK 301

Query: 303 YIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMA 362
           YI AI TGS+SQYIPLLN+YTNNLPI SDHYGS+ECFLGLNLDP+CDP E SYTLIPTMA
Sbjct: 302 YINAIVTGSMSQYIPLLNFYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTMA 361

Query: 363 YFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTNKA 422
           YFEFLPID TN NGEVTQ+LVDLVDVKLGQEYELVITTFAGLYR  +GDI+RVTGFT KA
Sbjct: 362 YFEFLPIDRTNPNGEVTQQLVDLVDVKLGQEYELVITTFAGLYRYRMGDIIRVTGFTKKA 421

Query: 423 PKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGHYV 482
           P  SFV RKNVVL+L  EKT+E  L   VE  G VLKPFGATIVDYTSYAD+STIPGHYV
Sbjct: 422 PNFSFVCRKNVVLSLNNEKTDEAGLHKAVEEGGHVLKPFGATIVDYTSYADSSTIPGHYV 481

Query: 483 LYWELLIDGNDHN-TQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIVK 542
           LY+EL+ D  +HN  Q ++ IPSSV+ DCCFAIEE+  + YR+ RSHEK I+PLEI+IVK
Sbjct: 482 LYFELIFD--EHNKAQISNSIPSSVYEDCCFAIEETLNSLYRLARSHEKCIDPLEIKIVK 541

Query: 543 SGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRV 596
           +GTFEKLM+LAIN GAS+ +YKTPR LNS QIH IQLLESN+V +YFSQK+P++
Sbjct: 542 AGTFEKLMQLAINRGASITRYKTPRCLNSKQIHIIQLLESNLVSNYFSQKSPKL 592

[Close]
BLAST of CSPI02G07750 vs. NCBI nr
Match: XP_008448232.1 (PREDICTED: indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo] >KAA0062188.1 indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo var. makuwa] >TYK04840.1 indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo var. makuwa])

HSP 1 Score: 884.4 bits (2284), Expect = 5.5e-253
Identity = 427/593 (72.01%), Postives = 494/593 (83.31%), Query Frame = 0 

Query: 3   SHVEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSST 62
           S+ EE WLDE+DKE LQ+I+ IT+ A EIQRQIL+EILS NANVEYLQ+HGL AST SST
Sbjct: 2   SNFEEKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSST 61

Query: 63  FKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFER 122
           FKKLIPLV Y+QL+PYI RIA+GDDS ILCSNPIT FF SSGTSG E KL+P+YE+EF R
Sbjct: 62  FKKLIPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFVR 121

Query: 123 RLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNLKS 182
           RLSFF+Y+  R K+LFPNINWHK K +NFHFAKP+ KTK GI+V ++F+ L KRS NL+S
Sbjct: 122 RLSFFSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLRS 181

Query: 183 VESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVD 242
           + SGNN  PDDI+ CT++YQSLYCQLLCGLYQND+VF+V A+ AS LIHVFKFLENHW+D
Sbjct: 182 MPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWID 241

Query: 243 LVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTK 302
           L +DIR  +I NPKIT  S+RESVMKI+VKPNP++ADLIE EC KG+W+GI+ +LWPN K
Sbjct: 242 LATDIRTRTI-NPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAK 301

Query: 303 YIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMA 362
           YI A+ TGS+SQYIPLLNYYTNNLPI SDHYGS+ECFLGLNLDP+C+P+E SYTLIPTMA
Sbjct: 302 YINAVVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTMA 361

Query: 363 YFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTNKA 422
           YFEFLPID  N  GEV Q LVDLVDVKLG EYELVITTFAGLYR  +GD+VRVTGF NKA
Sbjct: 362 YFEFLPIDMLNPKGEVNQRLVDLVDVKLGHEYELVITTFAGLYRYCMGDVVRVTGFKNKA 421

Query: 423 PKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGHYV 482
           P  SFVRRKNVVLN+  EKT+E  L   VE  G V+K FGATIVDYT+YAD STIPGHYV
Sbjct: 422 PNFSFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATIVDYTTYADISTIPGHYV 481

Query: 483 LYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIVKS 542
           LYWEL +D  D   Q  ++IP SVF DCC AIE S    YR+ RSHEK INPLEI+IVK+
Sbjct: 482 LYWELTLD--DLKEQNLNYIPCSVFEDCCLAIENSLNLLYRLARSHEKCINPLEIKIVKA 541

Query: 543 GTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRV 596
           GTFEKLM+LA+N GAS+ QYKTPR LNS+QIH IQLLESN+V +YFS+  P +
Sbjct: 542 GTFEKLMQLALNRGASITQYKTPRCLNSSQIHIIQLLESNLVSNYFSKNYPNL 591

[Close]
BLAST of CSPI02G07750 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 608.6 bits (1568), Expect = 5.4e-174
Identity = 312/598 (52.17%), Postives = 416/598 (69.57%), Query Frame = 0 

Query: 10  LDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSSTFKKLIPL 69
           LD+++K+ LQ I+ +TSNA ++QRQ+L EIL+ NA+VEYL++H L+  T   TFK ++P+
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 70  VSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRLSFFNY 129
           ++YE ++P I RIA GD SPIL S PI+ F  SSGTSGGE KL+P  E+E +RR   ++ 
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 130 LMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNLKSVES--GN 189
           LM    +  P +    GK M F F K + KT  G+    V +   K S   +       N
Sbjct: 137 LMPVMSQFVPGL--ENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTN 196

Query: 190 NAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVDLVSDI 249
              P++ + C+++YQS+Y Q+LCGL Q+  V +VGAV ASG I   KFLE HW++LV DI
Sbjct: 197 YTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDI 256

Query: 250 RRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTKYIKAI 309
           R G++++  ITD S+RE+V KIL KP+P+LAD +E EC K  W+GI+ +LWPNTKY+  I
Sbjct: 257 RTGTLSS-LITDPSVREAVAKIL-KPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVI 316

Query: 310 ATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMAYFEFL 369
            TG++SQYIP L+YY+N LP+    Y S+EC+ G+NL P+C P+E SYTLIP+MAYFEFL
Sbjct: 317 VTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFL 376

Query: 370 PIDTTN-----------INGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVT 429
           P+   N           +  +  QELVDLVDVKLGQEYELV+TT+AGL R  +GD++RVT
Sbjct: 377 PVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVT 436

Query: 430 GFTNKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTST 489
           GF NKAP+ SF+ RKNVVL+++ +KT+E +L+  V+NA   L PF A++ +YTSYADTS+
Sbjct: 437 GFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSS 496

Query: 490 IPGHYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLE 549
           IPGHYVL+WEL +DGN         IP SVF DCC A+EESF T YR  R  +K+I PLE
Sbjct: 497 IPGHYVLFWELCLDGNTP-------IPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLE 556

Query: 550 IRIVKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPR 595
           I+IV+ GTF+KLM  AI+ GAS+NQYKTPR +    I  I+LL S VV SYFS K P+
Sbjct: 557 IKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPI--IELLNSRVVDSYFSPKCPK 601

[Close]
BLAST of CSPI02G07750 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 599.0 bits (1543), Expect = 4.3e-171
Identity = 308/603 (51.08%), Postives = 414/603 (68.66%), Query Frame = 0 

Query: 5   VEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSSTFK 64
           V +  L E++K  LQ I+ +T+NA ++QR++L EILS NA+VEYL++HGL   T   TFK
Sbjct: 12  VSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFK 71

Query: 65  KLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRL 124
            ++P+V+YE ++P I RIA GD S +LCSNPI+ F  SSGTSGGE KL+P  E+E +RR 
Sbjct: 72  HIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRS 131

Query: 125 SFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRS--LNLKS 184
             ++ LM    +  P ++  KGK M F F K + KT  G+    V +   K S   N   
Sbjct: 132 LLYSLLMPVMDQFVPGLD--KGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPY 191

Query: 185 VESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVD 244
               N   P+  + C+++YQS+Y Q+LCGL Q+  V +VGAV ASG I   KFLE HW +
Sbjct: 192 DPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPE 251

Query: 245 LVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTK 304
           L  DIR G++++ +ITD S+RE+V +IL KP+P+LAD +E+EC K  W+GI+ +LWPNTK
Sbjct: 252 LARDIRTGTLSS-EITDSSVREAVGEIL-KPDPKLADFVESECRKTSWQGIITRLWPNTK 311

Query: 305 YIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMA 364
           Y+  I TG++SQYIP L+YY+N LP+    Y S+EC+ G+NL P+C P+E SYTLIP MA
Sbjct: 312 YVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMA 371

Query: 365 YFEFLPIDTTN-----------INGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGD 424
           YFEFLP+   +           +  +  QELVDLVDVKLGQEYELV+TT+AGLYR  +GD
Sbjct: 372 YFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGD 431

Query: 425 IVRVTGFTNKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSY 484
           ++ V GF N AP+ SF+ RKNVVL+++ +KT+E +L+  V+NA   L PF A++ +YTSY
Sbjct: 432 VLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSY 491

Query: 485 ADTSTIPGHYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKT 544
           ADTS+IPGHYVL+WEL ++GN         IP SVF DCC  IEES  + YR  R  +K+
Sbjct: 492 ADTSSIPGHYVLFWELCLNGNTP-------IPPSVFEDCCLTIEESLNSVYRQGRVSDKS 551

Query: 545 INPLEIRIVKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQK 595
           I PLEI++V+SGTF+KLM  AI+ GAS+NQYKTPR +    I  I+LL S VV SYFS K
Sbjct: 552 IGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPI--IELLNSRVVDSYFSPK 601

[Close]
BLAST of CSPI02G07750 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 515.0 bits (1325), Expect = 8.1e-146
Identity = 265/602 (44.02%), Postives = 386/602 (64.12%), Query Frame = 0 

Query: 1   MPSHVEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS 60
           + S +  +   E+D + L+ I+ +T N   +Q ++L EIL+ N+N EYL++  L      
Sbjct: 7   LQSRMVSATTSEKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDR 66

Query: 61  STFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEF 120
            TFK  +P+V+YE LKP I RI+ GD SPIL S+PIT F  SSGTS GE KL+P  E++ 
Sbjct: 67  KTFKSKVPVVTYEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDL 126

Query: 121 ERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNL 180
           +RR   ++ LM       P ++  KGK + F F K + KT  G+    V +   K     
Sbjct: 127 DRRQLLYSLLMPVMNLYVPGLD--KGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFK 186

Query: 181 KSVESGNNAI--PDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLEN 240
           +      N    P++ + C+++ QS+Y Q+LCGL     V ++GAV ASGL+    FL+N
Sbjct: 187 RRPYDPYNVYTSPNEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQN 246

Query: 241 HWVDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLW 300
           +W +L  DI  G++++ +I D +++  + KIL KP+ +LA+ +   CS+  W+GI+ K+W
Sbjct: 247 NWKELARDISTGTLSS-RIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIW 306

Query: 301 PNTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLI 360
           PNTKY+  I TG+++QYIP L YY+  LP+    Y S+E + G+NL P+C P+E SYT++
Sbjct: 307 PNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIM 366

Query: 361 PTMAYFEFLPIDTTNINGEVTQE-------LVDLVDVKLGQEYELVITTFAGLYRCSLGD 420
           P MAYFEFLP    N +G+   E       LV+L +V++G+EYELVITT+AGLYR  +GD
Sbjct: 367 PNMAYFEFLP---HNHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGD 426

Query: 421 IVRVTGFTNKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSY 480
           I+RVTGF N AP+  F+RRKNV+L++E +KT+E +L+  VENA  +    G  +++YTSY
Sbjct: 427 ILRVTGFHNSAPQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSY 486

Query: 481 ADTSTIPGHYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKT 540
           A+T TIPGHYV+YWELL  G D   Q+N  +   V   CC  +EES  + YR  R  +K+
Sbjct: 487 AETKTIPGHYVIYWELL--GRD---QSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKS 546

Query: 541 INPLEIRIVKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQK 594
           I PLEIR+V++GTFE+LM  AI+ GAS+NQYK PR ++   I  ++LL+S VV ++FS  
Sbjct: 547 IGPLEIRVVRNGTFEELMDYAISRGASINQYKVPRCVSFTPI--MELLDSRVVSAHFSPS 595

[Close]
BLAST of CSPI02G07750 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 510.8 bits (1314), Expect = 1.5e-144
Identity = 265/585 (45.30%), Postives = 379/585 (64.79%), Query Frame = 0 

Query: 12  ERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSSTFKKLIPLVS 71
           E+D + L+ I+ +T NA  +Q  +L+EIL+ NA+ EYL++  L  +T   TFK  IP+++
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 72  YEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRLSFFNYLM 131
           YE L+P I RIA+GD SPIL ++PI+ F  SSGTS GE KL+P  ++E +RR   ++ LM
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 132 ARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNLKSVESGNNAI- 191
                  P ++  KGK M F F K + KT  G+    V +   K            N   
Sbjct: 137 PVMNLYVPGLD--KGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYT 196

Query: 192 -PDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVDLVSDIRR 251
            P++ + C +++QS+Y Q+LCGL     V +VGAV ASGL+   +FL+ HW     DI  
Sbjct: 197 SPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIEL 256

Query: 252 GSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTKYIKAIAT 311
           G +++ +ITD S+R+ +  IL KP+P LA+ I  EC    W+ I+ ++WPNTKY+  I T
Sbjct: 257 GCLDS-EITDPSIRQCMSGIL-KPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVT 316

Query: 312 GSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMAYFEFLPI 371
           G+++QYIP L YY+  LP+    Y S+EC+ GLNL+P+  P+E SYT++P MAYFEF+P+
Sbjct: 317 GAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPL 376

Query: 372 DTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTNKAPKISFVR 431
             T        + V+LVDV +G+EYELV+TT+AGL R  +GDI+RVTGF N AP+  FVR
Sbjct: 377 GGT--------KAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVR 436

Query: 432 RKNVVLNLEYEKTNETDLRMGVENAGGVL-KPFGATIVDYTSYADTSTIPGHYVLYWELL 491
           RKNV+L+++ +KT+E++L+  VENA  +L +  G+ + +YTSYADTSTIPGHYVLYWELL
Sbjct: 437 RKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELL 496

Query: 492 IDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIVKSGTFEKL 551
           +   D   Q +H         CC  +EES  + YR  R  + ++ PLEIR+V++GTFE+L
Sbjct: 497 V--RDGARQPSH----ETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEEL 556

Query: 552 MKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNP 594
           M  AI+ GAS+NQYK PR +N   I  ++LL+S VV ++FS   P
Sbjct: 557 MDYAISRGASINQYKVPRCVNFTPI--VELLDSRVVSAHFSPSLP 581

[Close]
BLAST of CSPI02G07750 vs. TAIR 10
Match: AT5G13360.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 507.7 bits (1306), Expect = 1.3e-143
Identity = 262/583 (44.94%), Postives = 371/583 (63.64%), Query Frame = 0 

Query: 15  KEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSSTFKKLIPLVSYEQ 74
           K  L  ++ +T+N  +IQ  +L  ILS NA+ EYL    L+      +FKK +P+V+YE 
Sbjct: 11  KACLSLLEDVTTNVKQIQDSVLEAILSRNAHTEYLSGF-LNGQADKKSFKKNVPVVTYED 70

Query: 75  LKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRLSFFNYLMART 134
           +KPYI RIA G+ S ++C  PI+    SSGTSGG PKL+P+  +E E+R+SF +      
Sbjct: 71  IKPYIDRIANGEPSDLICDRPISVLLTSSGTSGGVPKLIPLTTEELEQRISFASLYRPLL 130

Query: 135 KELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNLKS-VESGNNAIPDD 194
            +    I   + K+   +F   + +T +GILV T+ + +LK      S +   +   P  
Sbjct: 131 YKYIEGI--RERKSFMLYFVTRESETASGILVRTMITCVLKSVTPANSFIWDQSQISPHA 190

Query: 195 ILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVDLVSDIRRGSIN 254
           I  C++T QS+YCQLLCGL Q D V ++GA  AS  + + KFLE+HW +  S+IR G ++
Sbjct: 191 ITTCSDTTQSMYCQLLCGLLQRDNVGRLGAPFASSFLKIIKFLEDHWPEFCSNIRTGCLS 250

Query: 255 NPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTKYIKAIATGSLS 314
           +  ITD      + K L  PNP+LA LIE ECS+  W+ IV +LWP  K I+AI TG+++
Sbjct: 251 D-WITDPQCVSGIGKFLTAPNPELASLIEQECSQTSWEAIVKRLWPKAKCIEAIVTGTMA 310

Query: 315 QYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMAYFEFLPIDTTN 374
           QY PLL +Y+  LP+ S  YGS+ECF GLNL+P+  PNE SYT+IP MAYFEFL ++   
Sbjct: 311 QYNPLLEFYSGGLPVISTFYGSSECFFGLNLNPLSKPNEVSYTIIPCMAYFEFLEVEKDY 370

Query: 375 INGEVTQE---LVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTNKAPKISFVRR 434
            +G    E   +VDLVDVK+G +YE V+TTFAGLYR  LGD++RVTGF N AP+  FV R
Sbjct: 371 ESGHDPAENPVVVDLVDVKIGHDYEPVVTTFAGLYRYRLGDVLRVTGFYNNAPQFHFVGR 430

Query: 435 KNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGHYVLYWELLID 494
           + VVL+++ +KT + DL   V NA  +L+P    ++D+TS  D+S+ PGHYVLYWEL   
Sbjct: 431 QKVVLSIDMDKTYDEDLLKAVTNASLLLEPHDLMLMDFTSRVDSSSYPGHYVLYWELGRK 490

Query: 495 GNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIVKSGTFEKLMK 554
             D   +    +  +V  +CCF IEES    YR  R ++K I PLEI++VK G F+KLM 
Sbjct: 491 VKDAKLE----LDQNVLEECCFTIEESLDAVYRKGRKNDKNIGPLEIKVVKPGAFDKLMN 550

Query: 555 LAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNP 594
             ++ G+S++QYKTPRS+ + +   +++LE+NVV  + SQK P
Sbjct: 551 FFLSRGSSVSQYKTPRSVTNEEA--LKILEANVVSEFLSQKTP 583

The following BLAST results are available for this feature:
BLAST of CSPI02G07750 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O818297.6e-17352.17Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q9LSQ46.0e-17051.08Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q8LQM51.8e-15347.00Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo... [more]
Q60EJ69.0e-15046.10Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo... [more]
A3BLS01.1e-14746.32Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399... [more]
BLAST of CSPI02G07750 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LHA00.0e+0099.66Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G081190 PE=3 SV=1[more]
A0A5D3BD930.0e+0088.80Indole-3-acetic acid-amido synthetase GH3.5-like OS=Cucumis melo var. makuwa OX=... [more]
A0A1S3B3T70.0e+0088.80indole-3-acetic acid-amido synthetase GH3.5-like OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A5A7UAR70.0e+0088.63Indole-3-acetic acid-amido synthetase GH3.5-like OS=Cucumis melo var. makuwa OX=... [more]
A0A5A7V3L52.6e-25372.01Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=... [more]
BLAST of CSPI02G07750 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004138974.10.0e+0099.66indole-3-acetic acid-amido synthetase GH3.5 isoform X1 [Cucumis sativus] >KGN613... [more]
XP_008441807.10.0e+0088.80PREDICTED: indole-3-acetic acid-amido synthetase GH3.5-like [Cucumis melo] >TYJ9... [more]
KAA0051296.10.0e+0088.63indole-3-acetic acid-amido synthetase GH3.5-like [Cucumis melo var. makuwa][more]
XP_038902067.12.9e-26275.08indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida][more]
XP_008448232.15.5e-25372.01PREDICTED: indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo] >KAA0... [more]
BLAST of CSPI02G07750 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G27260.15.4e-17452.17Auxin-responsive GH3 family protein [more]
AT5G54510.14.3e-17151.08Auxin-responsive GH3 family protein [more]
AT4G37390.18.1e-14644.02Auxin-responsive GH3 family protein [more]
AT2G14960.11.5e-14445.30Auxin-responsive GH3 family protein [more]
AT5G13360.11.3e-14344.94Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 21..571
e-value: 8.8E-180
score: 599.0
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 10..595
NoneNo IPR availablePANTHERPTHR31901:SF63INDOLE-3-ACETIC ACID-AMIDO SYNTHETASEcoord: 10..595

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G07750.1CSPI02G07750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0010279 indole-3-acetic acid amido synthetase activity