CSPI02G07720 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G07720
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationChr2: 6778266 .. 6780257 (+)
RNA-Seq ExpressionCSPI02G07720
SyntenyCSPI02G07720
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCATGGACCTCCTCAAGAAAGATCTCAAACAAGTTCTTTATAGAATCATCCAAGAACAAGATTTCGATTCCTCCGCCGCCGACGAAGCTATTCAGATATTGACTGCTTTGAAATTCTCTTTAATGGCCGCTTCTTCCTCCCAAGAATCCACCCGCTGTTCTGGCAGTAACACTCTCATTTTCCCCGACAAGTTCCGGTGTCCGATTTCCGGCGACCTCATGAAAGACCCTGTTCTCTTGATTACCGGTCAGGTTTGTGATTTTTCTCCTCTTATCATTTCTTTTTCTTTCCAATTGTCTTGGGCCTTCATAATATCTTTTTGTTTCAAGATTGTGTTGTTACTACCATGAATTTCCCTCTGTTGCCTCTGTTTTCTTTTTCAATTTTATGTAGCCTCTGTTCTTCTTTGTTCATCATTACGATCTTTTAGTCTTTTGAAAAGAAAGAAAAAAGATGATATTTATTTCTCCTATGGCTCTCCAGTGTCTAATTTTAATGATTGGAGTTTCTTTGTTTCATAAAGTTAACTTCATCTTTTTTGCAACGATATGTAAATTTGCATTGCCTCTTATATTACTTTCTTTTTTCTGTCTAAAAACATGTTTAAATATAATGAATCTATGTTCTCTAATCCAATGTCTAATTTCAGTGAGTGGGTCTTGTTTTAATTTTATGAGGGAAGTTGCTACAAATATGTATGAAAGGTTGGATAGATATAATAATCTACTGGTGTTTTAAGAAAAATTCTCTGTGAATTGTTGTGTGTGTGTGTGTGTGTTGCAGTTTGATTTTGTGTTTAATGTTCTTGAAACATGGTTTGGAATTCTAGACATATGATCGGTTCTTCATCGAAAAGTGGTTCCATGAAGGCCACAATACATGTCCTCAAACCAAGGAAGTCCTTACCGACATGACTCTTACCCCAAATCGTCTGCTTAGGTCGATGATCTCGCAATGGTGCCTGGATAACAGACTTGAACTTCCAAGGCTTAGTTATGAGGAAGAAGTTGACAATGTAACCGAATCCCATTTAGATGCATTGCTTGAGAAGTTGTTGTCTTCATCACTAATAGTTAAAAAAGAAGCTGCAAAGGAGTTGAGAGAGACAACAAGATGGAGCCATGAATTTCGAGCCCTTTTTGCAAAGCTCCCGGGGTCCGTTGAGAGATTATTGCATCCTCTGGTCTCAATTGGCAAAGTTGATCTCCATCCTGATCTTCTAGAGGATTTGATTACCACTATTCTCAATATCTCAGTTTTTGATGATAACAAGAAACATGTAGCGGAGAACCCGTTGGTCCTCCCTTTGCTGATTGAATCTTTACAGCACGGAAGTATTGAGTTGACAGCAAATGCAGTTGCAGCTATTTACTCATTATCTTTCAATGAGGCAAATAAGATAACGATGGGAAAAGTAGGAGTTTTCAAGCATCTGATTTCACTTTTAGACTATGCCCATCCTGGAGTGATAAGAGATGCTGGTTCAGCAATCTACAACCTCTGTACCACTGTTGAAAACAGAGAGAAGGCAGTCGGCAGTGGAGTAGTTGCCGCCATTTTTCGAAACATCGGCCGAAGCTTATTATTGGTCGATAAGTTGATTTTGATACTTGCACTTCTTTGCACCGATACGAAGGCTATTAACGAGATGTGCAAGTTTGATGCAGTGCCTTGTATGCTGAGGATCATAAGGGAAACTGAGTCCCAACGCATCAAAGAAAATTGTGCATCCATACTCTTTGCAATTTGTACAACTGATCAAAGCCAATTAAGGAGGATCCAGGAGGATGAAAATAAATATGAGACGATCTTGGAACTTTCAAAGATTGGTAATTCAAGGGCAAGGAGAAAGGCTACTGGCATACTCGATAGAATGCACAGTGCTGCTCCAAGAACTCACACTTCCTAATGAAAATGGGTCAGATTCTTTTACACCATTTTACTTGTGAATAGATATAAATGTACACAATGCGCTACAAATACAAAAG

mRNA sequence

ATGGCCATGGACCTCCTCAAGAAAGATCTCAAACAAGTTCTTTATAGAATCATCCAAGAACAAGATTTCGATTCCTCCGCCGCCGACGAAGCTATTCAGATATTGACTGCTTTGAAATTCTCTTTAATGGCCGCTTCTTCCTCCCAAGAATCCACCCGCTGTTCTGGCAGTAACACTCTCATTTTCCCCGACAAGTTCCGGTGTCCGATTTCCGGCGACCTCATGAAAGACCCTGTTCTCTTGATTACCGGTCAGACATATGATCGGTTCTTCATCGAAAAGTGGTTCCATGAAGGCCACAATACATGTCCTCAAACCAAGGAAGTCCTTACCGACATGACTCTTACCCCAAATCGTCTGCTTAGGTCGATGATCTCGCAATGGTGCCTGGATAACAGACTTGAACTTCCAAGGCTTAGTTATGAGGAAGAAGTTGACAATGTAACCGAATCCCATTTAGATGCATTGCTTGAGAAGTTGTTGTCTTCATCACTAATAGTTAAAAAAGAAGCTGCAAAGGAGTTGAGAGAGACAACAAGATGGAGCCATGAATTTCGAGCCCTTTTTGCAAAGCTCCCGGGGTCCGTTGAGAGATTATTGCATCCTCTGGTCTCAATTGGCAAAGTTGATCTCCATCCTGATCTTCTAGAGGATTTGATTACCACTATTCTCAATATCTCAGTTTTTGATGATAACAAGAAACATGTAGCGGAGAACCCGTTGGTCCTCCCTTTGCTGATTGAATCTTTACAGCACGGAAGTATTGAGTTGACAGCAAATGCAGTTGCAGCTATTTACTCATTATCTTTCAATGAGGCAAATAAGATAACGATGGGAAAAGTAGGAGTTTTCAAGCATCTGATTTCACTTTTAGACTATGCCCATCCTGGAGTGATAAGAGATGCTGGTTCAGCAATCTACAACCTCTGTACCACTGTTGAAAACAGAGAGAAGGCAGTCGGCAGTGGAGTAGTTGCCGCCATTTTTCGAAACATCGGCCGAAGCTTATTATTGGTCGATAAGTTGATTTTGATACTTGCACTTCTTTGCACCGATACGAAGGCTATTAACGAGATGTGCAAGTTTGATGCAGTGCCTTGTATGCTGAGGATCATAAGGGAAACTGAGTCCCAACGCATCAAAGAAAATTGTGCATCCATACTCTTTGCAATTTGTACAACTGATCAAAGCCAATTAAGGAGGATCCAGGAGGATGAAAATAAATATGAGACGATCTTGGAACTTTCAAAGATTGGTAATTCAAGGGCAAGGAGAAAGGCTACTGGCATACTCGATAGAATGCACAGTGCTGCTCCAAGAACTCACACTTCCTAATGAAAATGGGTCAGATTCTTTTACACCATTTTACTTGTGAATAGATATAAATGTACACAATGCGCTACAAATACAAAAG

Coding sequence (CDS)

ATGGCCATGGACCTCCTCAAGAAAGATCTCAAACAAGTTCTTTATAGAATCATCCAAGAACAAGATTTCGATTCCTCCGCCGCCGACGAAGCTATTCAGATATTGACTGCTTTGAAATTCTCTTTAATGGCCGCTTCTTCCTCCCAAGAATCCACCCGCTGTTCTGGCAGTAACACTCTCATTTTCCCCGACAAGTTCCGGTGTCCGATTTCCGGCGACCTCATGAAAGACCCTGTTCTCTTGATTACCGGTCAGACATATGATCGGTTCTTCATCGAAAAGTGGTTCCATGAAGGCCACAATACATGTCCTCAAACCAAGGAAGTCCTTACCGACATGACTCTTACCCCAAATCGTCTGCTTAGGTCGATGATCTCGCAATGGTGCCTGGATAACAGACTTGAACTTCCAAGGCTTAGTTATGAGGAAGAAGTTGACAATGTAACCGAATCCCATTTAGATGCATTGCTTGAGAAGTTGTTGTCTTCATCACTAATAGTTAAAAAAGAAGCTGCAAAGGAGTTGAGAGAGACAACAAGATGGAGCCATGAATTTCGAGCCCTTTTTGCAAAGCTCCCGGGGTCCGTTGAGAGATTATTGCATCCTCTGGTCTCAATTGGCAAAGTTGATCTCCATCCTGATCTTCTAGAGGATTTGATTACCACTATTCTCAATATCTCAGTTTTTGATGATAACAAGAAACATGTAGCGGAGAACCCGTTGGTCCTCCCTTTGCTGATTGAATCTTTACAGCACGGAAGTATTGAGTTGACAGCAAATGCAGTTGCAGCTATTTACTCATTATCTTTCAATGAGGCAAATAAGATAACGATGGGAAAAGTAGGAGTTTTCAAGCATCTGATTTCACTTTTAGACTATGCCCATCCTGGAGTGATAAGAGATGCTGGTTCAGCAATCTACAACCTCTGTACCACTGTTGAAAACAGAGAGAAGGCAGTCGGCAGTGGAGTAGTTGCCGCCATTTTTCGAAACATCGGCCGAAGCTTATTATTGGTCGATAAGTTGATTTTGATACTTGCACTTCTTTGCACCGATACGAAGGCTATTAACGAGATGTGCAAGTTTGATGCAGTGCCTTGTATGCTGAGGATCATAAGGGAAACTGAGTCCCAACGCATCAAAGAAAATTGTGCATCCATACTCTTTGCAATTTGTACAACTGATCAAAGCCAATTAAGGAGGATCCAGGAGGATGAAAATAAATATGAGACGATCTTGGAACTTTCAAAGATTGGTAATTCAAGGGCAAGGAGAAAGGCTACTGGCATACTCGATAGAATGCACAGTGCTGCTCCAAGAACTCACACTTCCTAA

Protein sequence

MAMDLLKKDLKQVLYRIIQEQDFDSSAADEAIQILTALKFSLMAASSSQESTRCSGSNTLIFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRLLRSMISQWCLDNRLELPRLSYEEEVDNVTESHLDALLEKLLSSSLIVKKEAAKELRETTRWSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDDNKKHVAENPLVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLLDYAHPGVIRDAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCTDTKAINEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRRIQEDENKYETILELSKIGNSRARRKATGILDRMHSAAPRTHTS*
Homology
BLAST of CSPI02G07720 vs. ExPASy Swiss-Prot
Match: Q9SRT0 (U-box domain-containing protein 9 OS=Arabidopsis thaliana OX=3702 GN=PUB9 PE=1 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 3.7e-92
Identity = 199/449 (44.32%), Postives = 287/449 (63.92%), Query Frame = 0

Query: 6   LKKDLKQVLYRIIQEQDFDSSAADEAIQILTALK---FSLMAASSSQESTRCSGSNTLIF 65
           LK+++K++L +I  E D      D+    L+AL+      MA SSS E        T+  
Sbjct: 20  LKREMKKLLIKIDDEDDLGVQTIDQLQDALSALREATMRKMAKSSSLEML-----ETVSC 79

Query: 66  PDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRLLR 125
           P++FRCP+S +LM+DPV+L +GQTYD+ FI+KW   G+ TCP+T++VL    LTPN L+R
Sbjct: 80  PEEFRCPLSNELMRDPVVLASGQTYDKLFIQKWLSSGNRTCPKTQQVLPHTALTPNLLIR 139

Query: 126 SMISQWCLDNRLEL-----PRLSYEEEVDNVTESHL-DALLEKLLSSSLIVKKEAAKELR 185
            MIS+WC  N LE      P L  E+E    ++  + ++LL K+ SS+L  +K AAKELR
Sbjct: 140 EMISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELR 199

Query: 186 ETTRWSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDD-NKKH 245
             TR   EFRALF + P  + RL++PL+     D    L ED++TT+LNIS+ DD NKK 
Sbjct: 200 LLTRKGTEFRALFGESPDEITRLVNPLLHGSNPD--EKLQEDVVTTLLNISIHDDSNKKL 259

Query: 246 VAENPLVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLLDYAH 305
           V ENP V+PLLI++L+ G++   +NA AAI++LS  ++NK+ +GK G+ K LI LL+  +
Sbjct: 260 VCENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEGN 319

Query: 306 PGVIRDAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCTDTKA 365
           P  I+D  +AI+ LC   ENR +AV  G V  + + I    L VD+L+ ILA+L T  KA
Sbjct: 320 PLAIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNG-LYVDELLAILAMLVTHWKA 379

Query: 366 INEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRRIQEDENKYETILEL 425
           + E+ +   V  +L+I RE+E +R KEN   IL  IC +D+++ + I+E+EN + TI +L
Sbjct: 380 VEELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKL 439

Query: 426 SKIGNSRARRKATGILDRMHSAAPRTHTS 445
           S+ G SRA+RKA GILDR+  A   THT+
Sbjct: 440 SREGTSRAQRKANGILDRLRKAMNLTHTA 460

BLAST of CSPI02G07720 vs. ExPASy Swiss-Prot
Match: Q3E9F7 (Putative U-box domain-containing protein 46 OS=Arabidopsis thaliana OX=3702 GN=PUB46 PE=3 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 4.9e-60
Identity = 158/445 (35.51%), Postives = 260/445 (58.43%), Query Frame = 0

Query: 4   DLLKKDLKQVLYRIIQE---QDFDSS--------AADEAIQILTALKFSLMAASSSQEST 63
           D L+++L++VL  I+ +    D D +        A DEA++ILT L+         +   
Sbjct: 10  DTLRRELQKVLTEILNDGGGNDRDETEAFSGVVKAIDEAVRILTCLR-------KVESKI 69

Query: 64  RCSGSNTLIFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWF-HEGHNTCPQTKEVLT 123
             S  + +  P +F C +S  +M +PV++ +GQTY++ +I +W  HE   TCP+TK+VL+
Sbjct: 70  PESDISPVEVPKEFICTLSNTIMIEPVIIASGQTYEKRYITEWLKHE--RTCPKTKQVLS 129

Query: 124 DMTLTPNRLLRSMISQWCLDNRLELPRLSYEEEVDNVTESHLDALLEKLLSSSLIVKK-E 183
                PN L+  +I+QWCL N+ +  + S +E V  +  S ++ALL+++ SSS +  + E
Sbjct: 130 HRLWIPNHLISDLITQWCLVNKYDHQKPS-DELVAELFTSDIEALLQRVSSSSSVADQIE 189

Query: 184 AAKELRETTRWSHEFRALF-AKLPGSVERLLHPLVSIGK-VDLHPDLLEDLITTILNISV 243
           AAKELR  T+     R  F A +  S+ RLL PL ++ + VD   +L E+++T + N+S+
Sbjct: 190 AAKELRHQTKKFPNVRVFFVAGIHDSITRLLSPLSTLDEAVDSSLELQENIVTALFNLSI 249

Query: 244 FDDNKKHVAENPLVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLI 303
            + NK  +AEN LV+PLL +SL+ G+ E   NA A + SLS  ++NKI +G     K LI
Sbjct: 250 LESNKTVIAENCLVIPLLTKSLKQGTDETRRNAAATLSSLSAIDSNKIIIGNSEAVKALI 309

Query: 304 SLLDYAHPGVIRDAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILAL 363
            L++       ++A S ++NLC  +EN+ K V +G++ A  + I ++   VD+L+ +LAL
Sbjct: 310 DLIEEGDLLATKEATSTVFNLCIVLENKGKVVSAGLIHAATKKI-KAGSNVDELLSLLAL 369

Query: 364 LCTDTKAINEMCKFDAVPCMLRIIRETESQRIKENCASILFAIC--TTDQSQLRRIQEDE 423
           + T  +A+ EM K   +  +  I+R+  S    EN   I+F +     D+S+L+ + E+E
Sbjct: 370 ISTHNRAVEEMDKLGFIYDLFSILRKPSSLLTGENAVVIVFNMYDRNRDRSRLKVVGEEE 429

Query: 424 NKYETILELSKIGNSRARRKATGIL 432
           N++ T  +L+K G+ RA RKA GIL
Sbjct: 430 NQHGTFTKLAKQGSVRAARKAQGIL 443

BLAST of CSPI02G07720 vs. ExPASy Swiss-Prot
Match: Q5VRH9 (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 5.3e-54
Identity = 131/384 (34.11%), Postives = 223/384 (58.07%), Query Frame = 0

Query: 61  IFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRL 120
           I PD+FRCPIS +LM+DPV++ +GQTY+R  I+KW   GH TCP+T++ L+  +LTPN +
Sbjct: 227 IIPDEFRCPISLELMQDPVIVSSGQTYERSCIQKWLDSGHKTCPKTQQPLSHTSLTPNFV 286

Query: 121 LRSMISQWCLDNRLELPRLSYEEEVDNVTESH------LDALLEKLLSSSLIVKKEAAKE 180
           L+S+ISQWC  N +ELP+           +S       L +L+ +L S +   ++ AA E
Sbjct: 287 LKSLISQWCEANGIELPKNKQNSRDKKAAKSSDYDHAGLVSLMNRLRSGNQDEQRAAAGE 346

Query: 181 LRETTRWSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDDNKK 240
           +R   + +   R   A+  G++  L++ L S       P   E  +T +LN+S+ ++NK 
Sbjct: 347 IRLLAKRNVNNRICIAE-AGAIPLLVNLLSS-----SDPRTQEHAVTALLNLSIHENNKA 406

Query: 241 HVAENPLVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLLDYA 300
            + ++   +P ++E L+ GS+E   NA A ++SLS  + NK+T+G  G    LI+LL   
Sbjct: 407 SIVDSH-AIPKIVEVLKTGSMETRENAAATLFSLSVVDENKVTIGAAGAIPPLINLLCDG 466

Query: 301 HPGVIRDAGSAIYNLCTTVENREKAVGSGVVAAIFR-NIGRSLLLVDKLILILALLCTDT 360
            P   +DA +AI+NLC    N+ +AV +G+V  +    +  +  ++D+ + +L++L  + 
Sbjct: 467 SPRGKKDAATAIFNLCIYQGNKVRAVKAGIVIHLMNFLVDPTGGMIDEALSLLSILAGNP 526

Query: 361 KAINEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRRIQEDENKYETIL 420
           +    + + + +P ++ +I+ T S R +EN A+IL+ +C+ D  Q    +      + + 
Sbjct: 527 EGKIVIARSEPIPPLVEVIK-TGSPRNRENAAAILWLLCSADTEQTLAAKA-AGVEDALK 586

Query: 421 ELSKIGNSRARRKATGILDRMHSA 438
           ELS+ G  RA+RKA+ IL+ MH A
Sbjct: 587 ELSETGTDRAKRKASSILELMHQA 601

BLAST of CSPI02G07720 vs. ExPASy Swiss-Prot
Match: Q8GUG9 (U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2 SV=2)

HSP 1 Score: 207.6 bits (527), Expect = 2.9e-52
Identity = 132/398 (33.17%), Postives = 230/398 (57.79%), Query Frame = 0

Query: 43  MAASSSQESTRCSGSNTLIFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNT 102
           M   ++ ES +   S+ L  P  F CP+S +LMKDPV++ TGQTY+R +I++W   G+ T
Sbjct: 225 MVNKNTDESKK---SDKLTIPVDFLCPVSLELMKDPVIVATGQTYERAYIQRWIDCGNLT 284

Query: 103 CPQTKEVLTDMTLTPNRLLRSMISQWCLDNRLELPRLSYEEEVDNVTE-SHLDALLEKLL 162
           CP+T++ L + TLTPN +LRS+IS+WC ++ +E P         N  + S + AL+++L 
Sbjct: 285 CPKTQQKLENFTLTPNYVLRSLISRWCAEHNIEQPAGYINGRTKNSGDMSVIRALVQRLS 344

Query: 163 SSSLIVKKEAAKELRETTRWSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLIT 222
           S S   ++ A  E+R  ++ S + R L A+  G++  L++ L S           E+ IT
Sbjct: 345 SRSTEDRRNAVSEIRSLSKRSTDNRILIAE-AGAIPVLVNLLTSEDVA-----TQENAIT 404

Query: 223 TILNISVFDDNKKHVAENPLVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKV 282
            +LN+S++++NK+ +     V   +++ L+ G++E   NA A ++SLS  + NKI +G  
Sbjct: 405 CVLNLSIYENNKELIMFAGAVTS-IVQVLRAGTMEARENAAATLFSLSLADENKIIIGGS 464

Query: 283 GVFKHLISLLDYAHPGVIRDAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSL--LLV 342
           G    L+ LL+   P   +DA +A++NLC    N+ +AV +G+V A+ + +  S    +V
Sbjct: 465 GAIPALVDLLENGTPRGKKDAATALFNLCIYHGNKGRAVRAGIVTALVKMLSDSTRHRMV 524

Query: 343 DKLILILALLCTDTKAINEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQL 402
           D+ + IL++L  +  A + + K + +P ++ I+ +T+  R +EN A+IL ++C  D  +L
Sbjct: 525 DEALTILSVLANNQDAKSAIVKANTLPALIGIL-QTDQTRNRENAAAILLSLCKRDTEKL 584

Query: 403 RRIQEDENKYETILELSKIGNSRARRKATGILDRMHSA 438
             I         +++LSK G  R +RKA  +L+ +  A
Sbjct: 585 ITIGR-LGAVVPLMDLSKNGTERGKRKAISLLELLRKA 610

BLAST of CSPI02G07720 vs. ExPASy Swiss-Prot
Match: Q9ZV31 (U-box domain-containing protein 12 OS=Arabidopsis thaliana OX=3702 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 4.9e-52
Identity = 135/398 (33.92%), Postives = 220/398 (55.28%), Query Frame = 0

Query: 47  SSQESTRCSGSNTLIFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQT 106
           SS   +R    + LI P++FRCPIS +LM DPV++ +GQTY+R  I+KW   GH TCP+T
Sbjct: 241 SSLPKSRDDDRDMLIPPEEFRCPISLELMTDPVIVSSGQTYERECIKKWLEGGHLTCPKT 300

Query: 107 KEVLTDMTLTPNRLLRSMISQWCLDNRLELP----------RLSYEEEVDNVTESHLDAL 166
           +E LT   +TPN +LRS+I+QWC  N +E P          + S      +   + ++ L
Sbjct: 301 QETLTSDIMTPNYVLRSLIAQWCESNGIEPPKRPNISQPSSKASSSSSAPDDEHNKIEEL 360

Query: 167 LEKLLSSSLIVKKEAAKELRETTRWSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLL 226
           L KL S     ++ AA E+R   + ++  R   A   G++  L++ L     +       
Sbjct: 361 LLKLTSQQPEDRRSAAGEIRLLAKQNNHNRVAIA-ASGAIPLLVNLLT----ISNDSRTQ 420

Query: 227 EDLITTILNISVFDDNKKHVAENPLVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKI 286
           E  +T+ILN+S+  +NK  +  +   +P ++  LQ GS+E   NA A ++SLS  + NK+
Sbjct: 421 EHAVTSILNLSICQENKGKIVYSSGAVPGIVHVLQKGSMEARENAAATLFSLSVIDENKV 480

Query: 287 TMGKVGVFKHLISLLDYAHPGVIRDAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGR-S 346
           T+G  G    L++LL        +DA +A++NLC    N+ KAV +G+V  + R +    
Sbjct: 481 TIGAAGAIPPLVTLLSEGSQRGKKDAATALFNLCIFQGNKGKAVRAGLVPVLMRLLTEPE 540

Query: 347 LLLVDKLILILALLCTDTKAINEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTD 406
             +VD+ + ILA+L +     +E+   DAVP ++  IR + S R KEN A++L  +C+ +
Sbjct: 541 SGMVDESLSILAILSSHPDGKSEVGAADAVPVLVDFIR-SGSPRNKENSAAVLVHLCSWN 600

Query: 407 QSQLRRIQEDENKYETILELSKIGNSRARRKATGILDR 434
           Q  L   Q+     + ++E+++ G  R +RKA  +L+R
Sbjct: 601 QQHLIEAQK-LGIMDLLIEMAENGTDRGKRKAAQLLNR 631

BLAST of CSPI02G07720 vs. ExPASy TrEMBL
Match: A0A0A0LHP1 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G080180 PE=4 SV=1)

HSP 1 Score: 857.8 bits (2215), Expect = 2.0e-245
Identity = 442/444 (99.55%), Postives = 443/444 (99.77%), Query Frame = 0

Query: 1   MAMDLLKKDLKQVLYRIIQEQDFDSSAADEAIQILTALKFSLMAASSSQESTRCSGSNTL 60
           MAMDLLKKDLKQVLYRIIQEQDFDSSAADEAIQILTALKFSLMAASSSQESTRCSGSNTL
Sbjct: 1   MAMDLLKKDLKQVLYRIIQEQDFDSSAADEAIQILTALKFSLMAASSSQESTRCSGSNTL 60

Query: 61  IFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRL 120
           IFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQT EVLTDMTLTPNRL
Sbjct: 61  IFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTNEVLTDMTLTPNRL 120

Query: 121 LRSMISQWCLDNRLELPRLSYEEEVDNVTESHLDALLEKLLSSSLIVKKEAAKELRETTR 180
           LRSMISQWCLDNRLELPRLSYEEEVDNVTESHLDALLEKLLSSSLIVKKEAAKELRETTR
Sbjct: 121 LRSMISQWCLDNRLELPRLSYEEEVDNVTESHLDALLEKLLSSSLIVKKEAAKELRETTR 180

Query: 181 WSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDDNKKHVAENP 240
           WSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDDNKKHVAENP
Sbjct: 181 WSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDDNKKHVAENP 240

Query: 241 LVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLLDYAHPGVIR 300
           LVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLLDYAHPGVIR
Sbjct: 241 LVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLLDYAHPGVIR 300

Query: 301 DAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCTDTKAINEMC 360
           DAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCTDTKAINEMC
Sbjct: 301 DAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCTDTKAINEMC 360

Query: 361 KFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRRIQEDENKYETILELSKIGN 420
           KFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLR+IQEDENKYETILELSKIGN
Sbjct: 361 KFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRKIQEDENKYETILELSKIGN 420

Query: 421 SRARRKATGILDRMHSAAPRTHTS 445
           SRARRKATGILDRMHSAAPRTHTS
Sbjct: 421 SRARRKATGILDRMHSAAPRTHTS 444

BLAST of CSPI02G07720 vs. ExPASy TrEMBL
Match: A0A5A7UZN6 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold165G00400 PE=4 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 7.8e-226
Identity = 409/444 (92.12%), Postives = 421/444 (94.82%), Query Frame = 0

Query: 1   MAMDLLKKDLKQVLYRIIQEQDFDSSAADEAIQILTALKFSLMAASSSQESTRCSGSNTL 60
           MAMDLLKKDLKQVLYRIIQE+DFDSSAAD+AIQILT+LKFSLM A SSQES  CSGS TL
Sbjct: 1   MAMDLLKKDLKQVLYRIIQEEDFDSSAADDAIQILTSLKFSLMPAPSSQESPGCSGSKTL 60

Query: 61  IFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRL 120
           I PDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRL
Sbjct: 61  IIPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRL 120

Query: 121 LRSMISQWCLDNRLELPRLSYEEEVDNVTESHLDALLEKLLSSSLIVKKEAAKELRETTR 180
           LRSMISQWCLDNR ELPRLSYEEEVDNVTESHLDALL +L+SSSLIVKKEAAKELRETTR
Sbjct: 121 LRSMISQWCLDNRFELPRLSYEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTR 180

Query: 181 WSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDDNKKHVAENP 240
            SHEFRALFAKLPGSVERLLHPL+SIGKVDLHPDL EDLITTILNISVFDDNK+HV ENP
Sbjct: 181 CSHEFRALFAKLPGSVERLLHPLISIGKVDLHPDLQEDLITTILNISVFDDNKEHVVENP 240

Query: 241 LVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLLDYAHPGVIR 300
           LVLPLLIESLQHGSIELTANAVAAIYSLSF+EANKITMGKVG+FKHLISLLDYAHPGV R
Sbjct: 241 LVLPLLIESLQHGSIELTANAVAAIYSLSFSEANKITMGKVGIFKHLISLLDYAHPGVTR 300

Query: 301 DAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCTDTKAINEMC 360
           DAGSAIYNLCTTVENREKAVGSGVVAAIFRNI RSLLLVDKLI ILALLCTD KAI+EMC
Sbjct: 301 DAGSAIYNLCTTVENREKAVGSGVVAAIFRNIARSLLLVDKLISILALLCTDVKAIDEMC 360

Query: 361 KFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRRIQEDENKYETILELSKIGN 420
           KFD VPCMLR IRETESQRIKENCASILFAICTTDQSQLR+IQEDENK ETILELSK GN
Sbjct: 361 KFDVVPCMLRFIRETESQRIKENCASILFAICTTDQSQLRKIQEDENKNETILELSKNGN 420

Query: 421 SRARRKATGILDRMHSAAPRTHTS 445
           SRA RKATGIL+RM  AA RTHTS
Sbjct: 421 SRASRKATGILERMQRAASRTHTS 444

BLAST of CSPI02G07720 vs. ExPASy TrEMBL
Match: A0A1S3B4X7 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485858 PE=4 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 7.8e-226
Identity = 409/444 (92.12%), Postives = 421/444 (94.82%), Query Frame = 0

Query: 1   MAMDLLKKDLKQVLYRIIQEQDFDSSAADEAIQILTALKFSLMAASSSQESTRCSGSNTL 60
           MAMDLLKKDLKQVLYRIIQE+DFDSSAAD+AIQILT+LKFSLM A SSQES  CSGS TL
Sbjct: 1   MAMDLLKKDLKQVLYRIIQEEDFDSSAADDAIQILTSLKFSLMPAPSSQESPGCSGSKTL 60

Query: 61  IFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRL 120
           I PDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRL
Sbjct: 61  IIPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRL 120

Query: 121 LRSMISQWCLDNRLELPRLSYEEEVDNVTESHLDALLEKLLSSSLIVKKEAAKELRETTR 180
           LRSMISQWCLDNR ELPRLSYEEEVDNVTESHLDALL +L+SSSLIVKKEAAKELRETTR
Sbjct: 121 LRSMISQWCLDNRFELPRLSYEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTR 180

Query: 181 WSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDDNKKHVAENP 240
            SHEFRALFAKLPGSVERLLHPL+SIGKVDLHPDL EDLITTILNISVFDDNK+HV ENP
Sbjct: 181 CSHEFRALFAKLPGSVERLLHPLISIGKVDLHPDLQEDLITTILNISVFDDNKEHVVENP 240

Query: 241 LVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLLDYAHPGVIR 300
           LVLPLLIESLQHGSIELTANAVAAIYSLSF+EANKITMGKVG+FKHLISLLDYAHPGV R
Sbjct: 241 LVLPLLIESLQHGSIELTANAVAAIYSLSFSEANKITMGKVGIFKHLISLLDYAHPGVTR 300

Query: 301 DAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCTDTKAINEMC 360
           DAGSAIYNLCTTVENREKAVGSGVVAAIFRNI RSLLLVDKLI ILALLCTD KAI+EMC
Sbjct: 301 DAGSAIYNLCTTVENREKAVGSGVVAAIFRNIARSLLLVDKLISILALLCTDVKAIDEMC 360

Query: 361 KFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRRIQEDENKYETILELSKIGN 420
           KFD VPCMLR IRETESQRIKENCASILFAICTTDQSQLR+IQEDENK ETILELSK GN
Sbjct: 361 KFDVVPCMLRFIRETESQRIKENCASILFAICTTDQSQLRKIQEDENKNETILELSKNGN 420

Query: 421 SRARRKATGILDRMHSAAPRTHTS 445
           SRA RKATGIL+RM  AA RTHTS
Sbjct: 421 SRASRKATGILERMQRAASRTHTS 444

BLAST of CSPI02G07720 vs. ExPASy TrEMBL
Match: A0A6J1HMF9 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111465532 PE=4 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 1.0e-140
Identity = 268/453 (59.16%), Postives = 342/453 (75.50%), Query Frame = 0

Query: 2   AMDLLKKDLKQVLYRIIQEQDFDSSAADEAIQILTALKFSLMAASSSQ----------ES 61
           +++LLKKD+K +L RI+ + DF+ S ADEAI+ L +LK + MAA+++           ES
Sbjct: 6   SLELLKKDIKDLLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAAAAAAAAAAAAPPLES 65

Query: 62  TRCSGSNTLIFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLT 121
              SG   L+ PD+FRCPISG+LM+DPVLL TGQTYDR FI KWF++GHNTCP+T EVL 
Sbjct: 66  GGDSGRTALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCPRTNEVLH 125

Query: 122 DMTLTPNRLLRSMISQWCLDNRLELPRLSYEEEVDNVTESHLDALLEKLLSSSLIVKKEA 181
           DMTLTPNR LRSMI +WC+DN+LELP+L+YEE V + TESHLD LL +LLSSSL  +K A
Sbjct: 126 DMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSSLSDQKLA 185

Query: 182 AKELRETTRWSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDD 241
           AKELR+  +W+HEFR+LFAKLP S+ERLLHP +S+  V+LHPDL EDLITT+LNIS+  D
Sbjct: 186 AKELRQIAKWNHEFRSLFAKLPDSIERLLHP-ISVDHVNLHPDLQEDLITTVLNISLSGD 245

Query: 242 NKKHVAENPLVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLL 301
           NKK + EN LVL LLI+SLQ+GS E  ANA AAI+SLS  + NKI +G  G  KHL+ LL
Sbjct: 246 NKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIKHLVGLL 305

Query: 302 DYAHPGVIRDAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCT 361
           D  HP V+RDA SAI++LCT   N+EKAV SGVV ++ RNI    +LVD+L+ +LALL  
Sbjct: 306 DEGHPVVMRDAASAIFSLCTASANKEKAVSSGVVVSVLRNITEG-VLVDELVSVLALLSN 365

Query: 362 DTKAINEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRRIQEDENKYET 421
           + KAI+ MCK +AV CMLR+IRE+ SQ +KENC SIL  I +T +S+L+ IQEDEN    
Sbjct: 366 NMKAISIMCKLNAVSCMLRVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDENANGM 425

Query: 422 ILELSKIGNSRARRKATGILDRMHSAAPRTHTS 445
           I +LS+ GNSRA+RKA  +L+++  A   THT+
Sbjct: 426 ISKLSRNGNSRAKRKAMSVLEKLQRAGSITHTA 456

BLAST of CSPI02G07720 vs. ExPASy TrEMBL
Match: A0A6J1FC00 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111444033 PE=4 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 2.9e-140
Identity = 267/452 (59.07%), Postives = 341/452 (75.44%), Query Frame = 0

Query: 2   AMDLLKKDLKQVLYRIIQEQDFDSSAADEAIQILTALKFSLMAASSSQ---------EST 61
           +++LLKKD+K++L RI+ + DF+ S ADEAI+ L +LK + MAA+S+          ES 
Sbjct: 6   SLELLKKDIKELLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAASAAAAAAAAAPVESG 65

Query: 62  RCSGSNTLIFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTD 121
             S    L+ PD+FRCPISG+LM+DPVLL TGQTYDR FI KWF++GHNTCP+T EVL D
Sbjct: 66  GDSARTALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCPRTNEVLHD 125

Query: 122 MTLTPNRLLRSMISQWCLDNRLELPRLSYEEEVDNVTESHLDALLEKLLSSSLIVKKEAA 181
           MTLTPNR LRSMI +WC+DN+LELP+L+YEE V + TESHLD LL +LLSSSL  +K AA
Sbjct: 126 MTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSSLSDQKLAA 185

Query: 182 KELRETTRWSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDDN 241
           KELR+  +W+HEFR+LFAKLP S+ERLLHP +S+  V+LHPDL EDLITT+LNIS+  DN
Sbjct: 186 KELRQIAKWNHEFRSLFAKLPDSIERLLHP-ISVDHVNLHPDLQEDLITTVLNISLSGDN 245

Query: 242 KKHVAENPLVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLLD 301
           KK + EN LVL LLI+SLQ+GS E  ANA AAI+SLS  + NKI +G  G  KHL+ LLD
Sbjct: 246 KKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIKHLVGLLD 305

Query: 302 YAHPGVIRDAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCTD 361
             HP V+RDA SAI+ LCT   N+EKAV SGVV ++ RNI    +LVD+L+ +LALL  +
Sbjct: 306 EGHPVVMRDAASAIFTLCTASANKEKAVSSGVVVSVLRNITEG-VLVDELVSVLALLSNN 365

Query: 362 TKAINEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRRIQEDENKYETI 421
            KAI+ MCK +AV CML++IRE+ SQ +KENC SIL  I +T +S+L+ IQEDEN    I
Sbjct: 366 MKAISIMCKLNAVSCMLKVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDENANGMI 425

Query: 422 LELSKIGNSRARRKATGILDRMHSAAPRTHTS 445
            +LS+ GNSRA+RKA  +L+++  A   THT+
Sbjct: 426 SKLSRNGNSRAKRKAMSVLEKLQRAGSITHTA 455

BLAST of CSPI02G07720 vs. NCBI nr
Match: XP_004152860.1 (U-box domain-containing protein 9 [Cucumis sativus] >KGN61318.1 hypothetical protein Csa_006758 [Cucumis sativus])

HSP 1 Score: 857.8 bits (2215), Expect = 4.1e-245
Identity = 442/444 (99.55%), Postives = 443/444 (99.77%), Query Frame = 0

Query: 1   MAMDLLKKDLKQVLYRIIQEQDFDSSAADEAIQILTALKFSLMAASSSQESTRCSGSNTL 60
           MAMDLLKKDLKQVLYRIIQEQDFDSSAADEAIQILTALKFSLMAASSSQESTRCSGSNTL
Sbjct: 1   MAMDLLKKDLKQVLYRIIQEQDFDSSAADEAIQILTALKFSLMAASSSQESTRCSGSNTL 60

Query: 61  IFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRL 120
           IFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQT EVLTDMTLTPNRL
Sbjct: 61  IFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTNEVLTDMTLTPNRL 120

Query: 121 LRSMISQWCLDNRLELPRLSYEEEVDNVTESHLDALLEKLLSSSLIVKKEAAKELRETTR 180
           LRSMISQWCLDNRLELPRLSYEEEVDNVTESHLDALLEKLLSSSLIVKKEAAKELRETTR
Sbjct: 121 LRSMISQWCLDNRLELPRLSYEEEVDNVTESHLDALLEKLLSSSLIVKKEAAKELRETTR 180

Query: 181 WSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDDNKKHVAENP 240
           WSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDDNKKHVAENP
Sbjct: 181 WSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDDNKKHVAENP 240

Query: 241 LVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLLDYAHPGVIR 300
           LVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLLDYAHPGVIR
Sbjct: 241 LVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLLDYAHPGVIR 300

Query: 301 DAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCTDTKAINEMC 360
           DAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCTDTKAINEMC
Sbjct: 301 DAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCTDTKAINEMC 360

Query: 361 KFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRRIQEDENKYETILELSKIGN 420
           KFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLR+IQEDENKYETILELSKIGN
Sbjct: 361 KFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRKIQEDENKYETILELSKIGN 420

Query: 421 SRARRKATGILDRMHSAAPRTHTS 445
           SRARRKATGILDRMHSAAPRTHTS
Sbjct: 421 SRARRKATGILDRMHSAAPRTHTS 444

BLAST of CSPI02G07720 vs. NCBI nr
Match: XP_008441805.1 (PREDICTED: U-box domain-containing protein 9-like [Cucumis melo] >KAA0061413.1 U-box domain-containing protein 9-like [Cucumis melo var. makuwa] >TYK28671.1 U-box domain-containing protein 9-like [Cucumis melo var. makuwa])

HSP 1 Score: 792.7 bits (2046), Expect = 1.6e-225
Identity = 409/444 (92.12%), Postives = 421/444 (94.82%), Query Frame = 0

Query: 1   MAMDLLKKDLKQVLYRIIQEQDFDSSAADEAIQILTALKFSLMAASSSQESTRCSGSNTL 60
           MAMDLLKKDLKQVLYRIIQE+DFDSSAAD+AIQILT+LKFSLM A SSQES  CSGS TL
Sbjct: 1   MAMDLLKKDLKQVLYRIIQEEDFDSSAADDAIQILTSLKFSLMPAPSSQESPGCSGSKTL 60

Query: 61  IFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRL 120
           I PDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRL
Sbjct: 61  IIPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRL 120

Query: 121 LRSMISQWCLDNRLELPRLSYEEEVDNVTESHLDALLEKLLSSSLIVKKEAAKELRETTR 180
           LRSMISQWCLDNR ELPRLSYEEEVDNVTESHLDALL +L+SSSLIVKKEAAKELRETTR
Sbjct: 121 LRSMISQWCLDNRFELPRLSYEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTR 180

Query: 181 WSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDDNKKHVAENP 240
            SHEFRALFAKLPGSVERLLHPL+SIGKVDLHPDL EDLITTILNISVFDDNK+HV ENP
Sbjct: 181 CSHEFRALFAKLPGSVERLLHPLISIGKVDLHPDLQEDLITTILNISVFDDNKEHVVENP 240

Query: 241 LVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLLDYAHPGVIR 300
           LVLPLLIESLQHGSIELTANAVAAIYSLSF+EANKITMGKVG+FKHLISLLDYAHPGV R
Sbjct: 241 LVLPLLIESLQHGSIELTANAVAAIYSLSFSEANKITMGKVGIFKHLISLLDYAHPGVTR 300

Query: 301 DAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCTDTKAINEMC 360
           DAGSAIYNLCTTVENREKAVGSGVVAAIFRNI RSLLLVDKLI ILALLCTD KAI+EMC
Sbjct: 301 DAGSAIYNLCTTVENREKAVGSGVVAAIFRNIARSLLLVDKLISILALLCTDVKAIDEMC 360

Query: 361 KFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRRIQEDENKYETILELSKIGN 420
           KFD VPCMLR IRETESQRIKENCASILFAICTTDQSQLR+IQEDENK ETILELSK GN
Sbjct: 361 KFDVVPCMLRFIRETESQRIKENCASILFAICTTDQSQLRKIQEDENKNETILELSKNGN 420

Query: 421 SRARRKATGILDRMHSAAPRTHTS 445
           SRA RKATGIL+RM  AA RTHTS
Sbjct: 421 SRASRKATGILERMQRAASRTHTS 444

BLAST of CSPI02G07720 vs. NCBI nr
Match: XP_038890637.1 (U-box domain-containing protein 9-like [Benincasa hispida])

HSP 1 Score: 618.2 bits (1593), Expect = 5.4e-173
Identity = 323/444 (72.75%), Postives = 372/444 (83.78%), Query Frame = 0

Query: 3   MDLLKKDLKQVLYRIIQEQDFDSSAADEAIQILTALKFSLMAASSS--QESTRCSGSNTL 62
           MD+LKKDLK +L RI+ E D + SA DEAIQ L ALK S MAAS+S   ES+  SG+NT 
Sbjct: 1   MDVLKKDLKLLLQRIVDEDDLEFSAVDEAIQKLNALKISKMAASASPIPESSGSSGTNTP 60

Query: 63  IFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRL 122
           I P++FRCPISG+LM DPVLLITGQTYDRFFIE+WFHEGHNTCP+T EVL+DM++TPNRL
Sbjct: 61  IVPEEFRCPISGELMNDPVLLITGQTYDRFFIEQWFHEGHNTCPRTNEVLSDMSITPNRL 120

Query: 123 LRSMISQWCLDNRLELPRLSYEEEVDNVTESHLDALLEKLLSSSLIVKKEAAKELRETTR 182
           LRSMISQWCLDNRL  P+LSYEEEVDN TESHLD LL KL+S S+  K +AAKELRETTR
Sbjct: 121 LRSMISQWCLDNRLVPPKLSYEEEVDNATESHLDELLNKLMSLSITDKIDAAKELRETTR 180

Query: 183 WSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDDNKKHVAENP 242
            SHEFRALFAK P S+ERLL+PL+S+ K++L+P L EDLITT+ NIS+FD NKKHVAE+P
Sbjct: 181 LSHEFRALFAKFPKSIERLLYPLISLEKINLYPRLQEDLITTVHNISIFDGNKKHVAEHP 240

Query: 243 LVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLLDYAHPGVIR 302
           LVLPLLI+SLQ+GSIE  ANA A IY LS NEANKI +G  GVFKHLI+LLD+AH  VIR
Sbjct: 241 LVLPLLIQSLQYGSIEAIANAAATIYLLSLNEANKIMIGNAGVFKHLIALLDHAHLVVIR 300

Query: 303 DAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCTDTKAINEMC 362
           DA SAIYNLCT VENR K VG G VA+I RNIGR  LLVD+LI ILALL TD  AI+EMC
Sbjct: 301 DAASAIYNLCTAVENRVKVVGCGAVASILRNIGRR-LLVDELISILALLSTDVNAIDEMC 360

Query: 363 KFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRRIQEDENKYETILELSKIGN 422
           K +AVPCML IIRET+SQR+KENCA +L AICTTDQSQL++I++DEN+  TI ELSKIGN
Sbjct: 361 KLNAVPCMLGIIRETDSQRVKENCALMLLAICTTDQSQLKKIRKDENENGTISELSKIGN 420

Query: 423 SRARRKATGILDRMHSAAPRTHTS 445
           SRARRKA+ ILDR+H A  RTHT+
Sbjct: 421 SRARRKASSILDRLHRAGSRTHTA 443

BLAST of CSPI02G07720 vs. NCBI nr
Match: XP_023538150.1 (U-box domain-containing protein 9-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 512.3 bits (1318), Expect = 4.2e-141
Identity = 269/454 (59.25%), Postives = 343/454 (75.55%), Query Frame = 0

Query: 2   AMDLLKKDLKQVLYRIIQEQDFDSSAADEAIQILTALKFSLMAASSSQ-----------E 61
           +++LLKKD+K++L RI+ + DF+ S ADEAI+ L +LK + MAA+++            E
Sbjct: 6   SLELLKKDIKELLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAAAAAAAAAAAAAAPVE 65

Query: 62  STRCSGSNTLIFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVL 121
           S   SG   L+ PD+FRCPISG+LM+DPVLL TGQTYDR FI KWF++GHNTCP+T EVL
Sbjct: 66  SGGDSGRKALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCPRTNEVL 125

Query: 122 TDMTLTPNRLLRSMISQWCLDNRLELPRLSYEEEVDNVTESHLDALLEKLLSSSLIVKKE 181
            DMTLTPNR LRSMI +WC+DN+LELP+L+YEE V + TESHLD LL +LLSSSL  +K 
Sbjct: 126 HDMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSSLSDQKL 185

Query: 182 AAKELRETTRWSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFD 241
           AAKELR+  +W+HEFR+LFAKLP S+ERLLHP +S+  V+LHPDL EDLITT+LNIS+  
Sbjct: 186 AAKELRQIAKWNHEFRSLFAKLPDSIERLLHP-ISVDHVNLHPDLQEDLITTVLNISLSG 245

Query: 242 DNKKHVAENPLVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISL 301
           DNKK + EN LVL LLI+SLQ+GS E  ANA AAI+SLS  + NKI +G  G  KHL+ L
Sbjct: 246 DNKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIKHLVGL 305

Query: 302 LDYAHPGVIRDAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLC 361
           LD  HP V+RDA SAI+ LCT   N+EKAV SGVV ++ RNI    +LVD+L+ +LALL 
Sbjct: 306 LDEGHPVVMRDAASAIFTLCTASANKEKAVSSGVVVSVLRNITEG-VLVDELVSVLALLS 365

Query: 362 TDTKAINEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRRIQEDENKYE 421
            + KAI+ MCK +AV CMLR+IRE+ SQ +KENC SIL  I +T +S+L+ IQEDEN   
Sbjct: 366 NNMKAISIMCKLNAVSCMLRVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDENANG 425

Query: 422 TILELSKIGNSRARRKATGILDRMHSAAPRTHTS 445
            I +LS+ GNSRA+RKAT +L+++  A   THT+
Sbjct: 426 MISKLSRNGNSRAKRKATSVLEKLQRAGSITHTA 457

BLAST of CSPI02G07720 vs. NCBI nr
Match: XP_022965721.1 (U-box domain-containing protein 9-like [Cucurbita maxima])

HSP 1 Score: 510.0 bits (1312), Expect = 2.1e-140
Identity = 268/453 (59.16%), Postives = 342/453 (75.50%), Query Frame = 0

Query: 2   AMDLLKKDLKQVLYRIIQEQDFDSSAADEAIQILTALKFSLMAASSSQ----------ES 61
           +++LLKKD+K +L RI+ + DF+ S ADEAI+ L +LK + MAA+++           ES
Sbjct: 6   SLELLKKDIKDLLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAAAAAAAAAAAAPPLES 65

Query: 62  TRCSGSNTLIFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLT 121
              SG   L+ PD+FRCPISG+LM+DPVLL TGQTYDR FI KWF++GHNTCP+T EVL 
Sbjct: 66  GGDSGRTALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCPRTNEVLH 125

Query: 122 DMTLTPNRLLRSMISQWCLDNRLELPRLSYEEEVDNVTESHLDALLEKLLSSSLIVKKEA 181
           DMTLTPNR LRSMI +WC+DN+LELP+L+YEE V + TESHLD LL +LLSSSL  +K A
Sbjct: 126 DMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSSLSDQKLA 185

Query: 182 AKELRETTRWSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDD 241
           AKELR+  +W+HEFR+LFAKLP S+ERLLHP +S+  V+LHPDL EDLITT+LNIS+  D
Sbjct: 186 AKELRQIAKWNHEFRSLFAKLPDSIERLLHP-ISVDHVNLHPDLQEDLITTVLNISLSGD 245

Query: 242 NKKHVAENPLVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLL 301
           NKK + EN LVL LLI+SLQ+GS E  ANA AAI+SLS  + NKI +G  G  KHL+ LL
Sbjct: 246 NKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIKHLVGLL 305

Query: 302 DYAHPGVIRDAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCT 361
           D  HP V+RDA SAI++LCT   N+EKAV SGVV ++ RNI    +LVD+L+ +LALL  
Sbjct: 306 DEGHPVVMRDAASAIFSLCTASANKEKAVSSGVVVSVLRNITEG-VLVDELVSVLALLSN 365

Query: 362 DTKAINEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRRIQEDENKYET 421
           + KAI+ MCK +AV CMLR+IRE+ SQ +KENC SIL  I +T +S+L+ IQEDEN    
Sbjct: 366 NMKAISIMCKLNAVSCMLRVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDENANGM 425

Query: 422 ILELSKIGNSRARRKATGILDRMHSAAPRTHTS 445
           I +LS+ GNSRA+RKA  +L+++  A   THT+
Sbjct: 426 ISKLSRNGNSRAKRKAMSVLEKLQRAGSITHTA 456

BLAST of CSPI02G07720 vs. TAIR 10
Match: AT3G07360.1 (plant U-box 9 )

HSP 1 Score: 340.1 bits (871), Expect = 2.7e-93
Identity = 199/449 (44.32%), Postives = 287/449 (63.92%), Query Frame = 0

Query: 6   LKKDLKQVLYRIIQEQDFDSSAADEAIQILTALK---FSLMAASSSQESTRCSGSNTLIF 65
           LK+++K++L +I  E D      D+    L+AL+      MA SSS E        T+  
Sbjct: 20  LKREMKKLLIKIDDEDDLGVQTIDQLQDALSALREATMRKMAKSSSLEML-----ETVSC 79

Query: 66  PDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRLLR 125
           P++FRCP+S +LM+DPV+L +GQTYD+ FI+KW   G+ TCP+T++VL    LTPN L+R
Sbjct: 80  PEEFRCPLSNELMRDPVVLASGQTYDKLFIQKWLSSGNRTCPKTQQVLPHTALTPNLLIR 139

Query: 126 SMISQWCLDNRLEL-----PRLSYEEEVDNVTESHL-DALLEKLLSSSLIVKKEAAKELR 185
            MIS+WC  N LE      P L  E+E    ++  + ++LL K+ SS+L  +K AAKELR
Sbjct: 140 EMISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELR 199

Query: 186 ETTRWSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDD-NKKH 245
             TR   EFRALF + P  + RL++PL+     D    L ED++TT+LNIS+ DD NKK 
Sbjct: 200 LLTRKGTEFRALFGESPDEITRLVNPLLHGSNPD--EKLQEDVVTTLLNISIHDDSNKKL 259

Query: 246 VAENPLVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLLDYAH 305
           V ENP V+PLLI++L+ G++   +NA AAI++LS  ++NK+ +GK G+ K LI LL+  +
Sbjct: 260 VCENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEGN 319

Query: 306 PGVIRDAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCTDTKA 365
           P  I+D  +AI+ LC   ENR +AV  G V  + + I    L VD+L+ ILA+L T  KA
Sbjct: 320 PLAIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNG-LYVDELLAILAMLVTHWKA 379

Query: 366 INEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRRIQEDENKYETILEL 425
           + E+ +   V  +L+I RE+E +R KEN   IL  IC +D+++ + I+E+EN + TI +L
Sbjct: 380 VEELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKL 439

Query: 426 SKIGNSRARRKATGILDRMHSAAPRTHTS 445
           S+ G SRA+RKA GILDR+  A   THT+
Sbjct: 440 SREGTSRAQRKANGILDRLRKAMNLTHTA 460

BLAST of CSPI02G07720 vs. TAIR 10
Match: AT3G07360.2 (plant U-box 9 )

HSP 1 Score: 243.8 bits (621), Expect = 2.6e-64
Identity = 147/328 (44.82%), Postives = 211/328 (64.33%), Query Frame = 0

Query: 124 MISQWCLDNRLEL-----PRLSYEEEVDNVTESHL-DALLEKLLSSSLIVKKEAAKELRE 183
           MIS+WC  N LE      P L  E+E    ++  + ++LL K+ SS+L  +K AAKELR 
Sbjct: 1   MISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRL 60

Query: 184 TTRWSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDD-NKKHV 243
            TR   EFRALF + P  + RL++PL+     D    L ED++TT+LNIS+ DD NKK V
Sbjct: 61  LTRKGTEFRALFGESPDEITRLVNPLLHGSNPD--EKLQEDVVTTLLNISIHDDSNKKLV 120

Query: 244 AENPLVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLLDYAHP 303
            ENP V+PLLI++L+ G++   +NA AAI++LS  ++NK+ +GK G+ K LI LL+  +P
Sbjct: 121 CENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEGNP 180

Query: 304 GVIRDAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCTDTKAI 363
             I+D  +AI+ LC   ENR +AV  G V  + + I    L VD+L+ ILA+L T  KA+
Sbjct: 181 LAIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNG-LYVDELLAILAMLVTHWKAV 240

Query: 364 NEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRRIQEDENKYETILELS 423
            E+ +   V  +L+I RE+E +R KEN   IL  IC +D+++ + I+E+EN + TI +LS
Sbjct: 241 EELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKLS 300

Query: 424 KIGNSRARRKATGILDRMHSAAPRTHTS 445
           + G SRA+RKA GILDR+  A   THT+
Sbjct: 301 REGTSRAQRKANGILDRLRKAMNLTHTA 325

BLAST of CSPI02G07720 vs. TAIR 10
Match: AT3G07360.3 (plant U-box 9 )

HSP 1 Score: 243.8 bits (621), Expect = 2.6e-64
Identity = 147/328 (44.82%), Postives = 211/328 (64.33%), Query Frame = 0

Query: 124 MISQWCLDNRLEL-----PRLSYEEEVDNVTESHL-DALLEKLLSSSLIVKKEAAKELRE 183
           MIS+WC  N LE      P L  E+E    ++  + ++LL K+ SS+L  +K AAKELR 
Sbjct: 1   MISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRL 60

Query: 184 TTRWSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDD-NKKHV 243
            TR   EFRALF + P  + RL++PL+     D    L ED++TT+LNIS+ DD NKK V
Sbjct: 61  LTRKGTEFRALFGESPDEITRLVNPLLHGSNPD--EKLQEDVVTTLLNISIHDDSNKKLV 120

Query: 244 AENPLVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLLDYAHP 303
            ENP V+PLLI++L+ G++   +NA AAI++LS  ++NK+ +GK G+ K LI LL+  +P
Sbjct: 121 CENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEGNP 180

Query: 304 GVIRDAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCTDTKAI 363
             I+D  +AI+ LC   ENR +AV  G V  + + I    L VD+L+ ILA+L T  KA+
Sbjct: 181 LAIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNG-LYVDELLAILAMLVTHWKAV 240

Query: 364 NEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRRIQEDENKYETILELS 423
            E+ +   V  +L+I RE+E +R KEN   IL  IC +D+++ + I+E+EN + TI +LS
Sbjct: 241 EELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKLS 300

Query: 424 KIGNSRARRKATGILDRMHSAAPRTHTS 445
           + G SRA+RKA GILDR+  A   THT+
Sbjct: 301 REGTSRAQRKANGILDRLRKAMNLTHTA 325

BLAST of CSPI02G07720 vs. TAIR 10
Match: AT5G18320.1 (ARM repeat superfamily protein )

HSP 1 Score: 233.4 bits (594), Expect = 3.5e-61
Identity = 158/445 (35.51%), Postives = 260/445 (58.43%), Query Frame = 0

Query: 4   DLLKKDLKQVLYRIIQE---QDFDSS--------AADEAIQILTALKFSLMAASSSQEST 63
           D L+++L++VL  I+ +    D D +        A DEA++ILT L+         +   
Sbjct: 10  DTLRRELQKVLTEILNDGGGNDRDETEAFSGVVKAIDEAVRILTCLR-------KVESKI 69

Query: 64  RCSGSNTLIFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWF-HEGHNTCPQTKEVLT 123
             S  + +  P +F C +S  +M +PV++ +GQTY++ +I +W  HE   TCP+TK+VL+
Sbjct: 70  PESDISPVEVPKEFICTLSNTIMIEPVIIASGQTYEKRYITEWLKHE--RTCPKTKQVLS 129

Query: 124 DMTLTPNRLLRSMISQWCLDNRLELPRLSYEEEVDNVTESHLDALLEKLLSSSLIVKK-E 183
                PN L+  +I+QWCL N+ +  + S +E V  +  S ++ALL+++ SSS +  + E
Sbjct: 130 HRLWIPNHLISDLITQWCLVNKYDHQKPS-DELVAELFTSDIEALLQRVSSSSSVADQIE 189

Query: 184 AAKELRETTRWSHEFRALF-AKLPGSVERLLHPLVSIGK-VDLHPDLLEDLITTILNISV 243
           AAKELR  T+     R  F A +  S+ RLL PL ++ + VD   +L E+++T + N+S+
Sbjct: 190 AAKELRHQTKKFPNVRVFFVAGIHDSITRLLSPLSTLDEAVDSSLELQENIVTALFNLSI 249

Query: 244 FDDNKKHVAENPLVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLI 303
            + NK  +AEN LV+PLL +SL+ G+ E   NA A + SLS  ++NKI +G     K LI
Sbjct: 250 LESNKTVIAENCLVIPLLTKSLKQGTDETRRNAAATLSSLSAIDSNKIIIGNSEAVKALI 309

Query: 304 SLLDYAHPGVIRDAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILAL 363
            L++       ++A S ++NLC  +EN+ K V +G++ A  + I ++   VD+L+ +LAL
Sbjct: 310 DLIEEGDLLATKEATSTVFNLCIVLENKGKVVSAGLIHAATKKI-KAGSNVDELLSLLAL 369

Query: 364 LCTDTKAINEMCKFDAVPCMLRIIRETESQRIKENCASILFAIC--TTDQSQLRRIQEDE 423
           + T  +A+ EM K   +  +  I+R+  S    EN   I+F +     D+S+L+ + E+E
Sbjct: 370 ISTHNRAVEEMDKLGFIYDLFSILRKPSSLLTGENAVVIVFNMYDRNRDRSRLKVVGEEE 429

Query: 424 NKYETILELSKIGNSRARRKATGIL 432
           N++ T  +L+K G+ RA RKA GIL
Sbjct: 430 NQHGTFTKLAKQGSVRAARKAQGIL 443

BLAST of CSPI02G07720 vs. TAIR 10
Match: AT1G23030.1 (ARM repeat superfamily protein )

HSP 1 Score: 207.6 bits (527), Expect = 2.1e-53
Identity = 132/398 (33.17%), Postives = 230/398 (57.79%), Query Frame = 0

Query: 43  MAASSSQESTRCSGSNTLIFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNT 102
           M   ++ ES +   S+ L  P  F CP+S +LMKDPV++ TGQTY+R +I++W   G+ T
Sbjct: 225 MVNKNTDESKK---SDKLTIPVDFLCPVSLELMKDPVIVATGQTYERAYIQRWIDCGNLT 284

Query: 103 CPQTKEVLTDMTLTPNRLLRSMISQWCLDNRLELPRLSYEEEVDNVTE-SHLDALLEKLL 162
           CP+T++ L + TLTPN +LRS+IS+WC ++ +E P         N  + S + AL+++L 
Sbjct: 285 CPKTQQKLENFTLTPNYVLRSLISRWCAEHNIEQPAGYINGRTKNSGDMSVIRALVQRLS 344

Query: 163 SSSLIVKKEAAKELRETTRWSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLIT 222
           S S   ++ A  E+R  ++ S + R L A+  G++  L++ L S           E+ IT
Sbjct: 345 SRSTEDRRNAVSEIRSLSKRSTDNRILIAE-AGAIPVLVNLLTSEDVA-----TQENAIT 404

Query: 223 TILNISVFDDNKKHVAENPLVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKV 282
            +LN+S++++NK+ +     V   +++ L+ G++E   NA A ++SLS  + NKI +G  
Sbjct: 405 CVLNLSIYENNKELIMFAGAVTS-IVQVLRAGTMEARENAAATLFSLSLADENKIIIGGS 464

Query: 283 GVFKHLISLLDYAHPGVIRDAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSL--LLV 342
           G    L+ LL+   P   +DA +A++NLC    N+ +AV +G+V A+ + +  S    +V
Sbjct: 465 GAIPALVDLLENGTPRGKKDAATALFNLCIYHGNKGRAVRAGIVTALVKMLSDSTRHRMV 524

Query: 343 DKLILILALLCTDTKAINEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQL 402
           D+ + IL++L  +  A + + K + +P ++ I+ +T+  R +EN A+IL ++C  D  +L
Sbjct: 525 DEALTILSVLANNQDAKSAIVKANTLPALIGIL-QTDQTRNRENAAAILLSLCKRDTEKL 584

Query: 403 RRIQEDENKYETILELSKIGNSRARRKATGILDRMHSA 438
             I         +++LSK G  R +RKA  +L+ +  A
Sbjct: 585 ITIGR-LGAVVPLMDLSKNGTERGKRKAISLLELLRKA 610

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SRT03.7e-9244.32U-box domain-containing protein 9 OS=Arabidopsis thaliana OX=3702 GN=PUB9 PE=1 S... [more]
Q3E9F74.9e-6035.51Putative U-box domain-containing protein 46 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q5VRH95.3e-5434.11U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Q8GUG92.9e-5233.17U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2... [more]
Q9ZV314.9e-5233.92U-box domain-containing protein 12 OS=Arabidopsis thaliana OX=3702 GN=PUB12 PE=2... [more]
Match NameE-valueIdentityDescription
A0A0A0LHP12.0e-24599.55RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G080180 PE... [more]
A0A5A7UZN67.8e-22692.12RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3B4X77.8e-22692.12RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485858 PE=4 ... [more]
A0A6J1HMF91.0e-14059.16RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111465532 P... [more]
A0A6J1FC002.9e-14059.07RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111444033... [more]
Match NameE-valueIdentityDescription
XP_004152860.14.1e-24599.55U-box domain-containing protein 9 [Cucumis sativus] >KGN61318.1 hypothetical pro... [more]
XP_008441805.11.6e-22592.12PREDICTED: U-box domain-containing protein 9-like [Cucumis melo] >KAA0061413.1 U... [more]
XP_038890637.15.4e-17372.75U-box domain-containing protein 9-like [Benincasa hispida][more]
XP_023538150.14.2e-14159.25U-box domain-containing protein 9-like [Cucurbita pepo subsp. pepo][more]
XP_022965721.12.1e-14059.16U-box domain-containing protein 9-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G07360.12.7e-9344.32plant U-box 9 [more]
AT3G07360.22.6e-6444.82plant U-box 9 [more]
AT3G07360.32.6e-6444.82plant U-box 9 [more]
AT5G18320.13.5e-6135.51ARM repeat superfamily protein [more]
AT1G23030.12.1e-5333.17ARM repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000225ArmadilloSMARTSM00185arm_5coord: 271..311
e-value: 0.014
score: 24.6
coord: 229..270
e-value: 18.0
score: 11.2
coord: 352..393
e-value: 9.9
score: 13.3
IPR000225ArmadilloPFAMPF00514Armcoord: 271..310
e-value: 3.5E-5
score: 23.7
IPR003613U box domainSMARTSM00504Ubox_2coord: 65..128
e-value: 5.9E-28
score: 108.9
IPR003613U box domainPFAMPF04564U-boxcoord: 63..133
e-value: 1.7E-18
score: 66.6
IPR003613U box domainPROSITEPS51698U_BOXcoord: 61..135
score: 33.518124
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 62..135
e-value: 6.5E-26
score: 92.2
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 142..440
e-value: 2.1E-30
score: 107.8
NoneNo IPR availablePANTHERPTHR23315:SF265U-BOX DOMAIN-CONTAINING PROTEIN 46-RELATEDcoord: 3..438
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 3..438
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 66..108
e-value: 3.57474E-22
score: 86.9038
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 62..136
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 151..434

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G07720.1CSPI02G07720.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity