CSPI02G07160 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G07160
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionAuxin efflux carrier component
LocationChr2: 6001098 .. 6005370 (+)
RNA-Seq ExpressionCSPI02G07160
SyntenyCSPI02G07160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGCTCTAAAATCCAATAATAAGAAGTGTTGGGTATATTGTGGCCATGATCGGTTGGCAAGATGTTTATAAGGTGGTTGCCGCCATGGCTCCATTGTACTTTGCTTTGATCTTAGGCTATGGCTCTGTGAAATGGTGGAAAATCTTTAGCACTCAACAGTGCGACGCCATTAACAAATTAGTTTGCTATTTCACTCTCCCTCTCTTCACCTTCGACTTCACTTCCCATATCGACCCTTTTCATCTCAACTTCCCTTTCATCGCCGCCGACGCCATCGGAAAACTTATCATCGTTCTCGTCTTGGCCTTTTGGGCCAAGTGTACTACCAAAGGTAGTTATTGTTGGTCCATCACTAGCTTCTCTCTCTCTACGCTCACCAACGCCCTCGTCATTGGTGTTCCTCTCGCTAAGGTCATGTACGGTCAAATGGCTGTCGATCTCGTTGTACAGGCAAGATTTTAAACAATAATATTGATATGTTTTTATTACTAGGGTTGTATAGACGGGTGGTAATGTTGACATGTTATGAAAATTTTGAATTTTGAGTATGTAGGGTTCGGTGGTTCAAGCAATTGTTTGGTTGACTATACTATTGTTTGTGTTGGAACTTCGACGAACGGGGTTGGATTTGGTCGCTGCTGAAGCTTCTTCGGGGGTTGTGAGTGAAGAGAAAACTGTTGAAGTGGGTGGAGAGGGAGAAAAGGATTTAGAGGGAGAGGGCATGGAGGTTGAGGAAATGAGAAGATCAAGTAAGAGTCATAGGCCTTCTTTGAAGCCTTTGATGAAGAAAGTTTGGGTGAAGATGGCTGGGAATCCCAACTCATATGCTTGTACCATTGGCTTTGCTTGGGCATTTGTTGCAAAAAGGTATTTCTATATCATAAGAAAAACAAAAAGAATTAAACCATAGACCAAAAGAATAAAAAAGATTAATGAATTAAATAAAATCCACATAGTCTTTGAGCTTAAAAAATAGTTGCATTTGATAGTGCTTCTTCATTTTTTTTTTCTTTGAAATTTTAAGGTGAAATACTCTTATCAATGTGTAGTTTTTATACTTTTCCATAAATCTTAAAATCAATATTCATCATTAGTTTAATCTCTTATTTTTTAGGATTGTTATTACAATTTATAAAATACAATAACTAAATTGTTACTCGTTAAAGTTCAAACATAAAATTGTTAGATATTCATATTATTAAAGGACGACCGTTTGGCCCTCAGATCAGGAAGGTTTTAGCTTCTTCCAAGTTTGTCTCAAGATTAGGACTATTCTACTTTTTTTTCTTATTTATCCTATTCTTCACTCTCTCCTTTCACTATTTCCCACTTCTCCCTTTCACTATTCCTCACTCTCTCATTTAACTATTTCTTATTTTTCCAAGCATAATAATCTGCACCTCAAACACAGATTATAGTAAACCATAAATTTAAATTATAATAACTCACTTCACGCCTCAAACGCTTTCAGAGTCTGGAAATTAAACAAAACGTATAATTATATATATATATATGTGTGTGTGTGTTACAAGGTAATAATGTATGTATGTGTATATATACATATATATGAATGTTTTGAAAATTTGATGAAGGTGGCATGTTGAAATGCCAAGCATTATGGAGGGGTCAATTTTAATAATGTCAAAAGCTGGGATTGGCACTGCCATGTTCAACATGGGTAAGATTACAAACTTACTTCCTTTGTTAATTTTATTTTATGTTGCATCTTTCATTTATTACAAAATATATATGAAAAACTAAACGTCTAGTTTAGATTTATTAGAGTGAAATTTTCAAACAAATTCCATTTTGCAAAATTGCATCAAATTAATAAAAACATTTAGAAAAAAACAGCTCCATATCGTGAATATAACAAAATTAGTAATATCAAATAACTATCAAACAGTAATATTATAAAACGGACTATCAGACGATAATTATAGGACGATAGTCATTTTTTAAAATTTACTAATTTTACAAATTTATAAAATATTGTGATATATATTAGTTTTTTTTAATTTTTAAAATTAAATTAATTTAAAAGTAAATAAAAAAAAATTCAACTGGTTGTAAGCTCTTTCAATTTTTTTTCTCAATCACTTATTTACAAAATTAAACCACCTGACCTAATTCTATATATTTAATAATCATGTTATTTAAGGATGATTTGACTTGTTATATTTAGTTTATAAACTTTTTTTAACTAGATTATATTATAACTAAAATTTCTATAGTGTCCAGCAATTCCCATTAAAAATTTTAATTCTCATTCTTTTTTGAAGAAAAAGATTAGTGGGTATAATTGAAGATTTGAGTGGGAATCTATTCAAAAAAATATATGTGACCTAAGCTAATAAAAATAATATTAATGGGATCTTAGGTTAGTATATTTGTTTACAATTAACAAAAGTTAAAATCAAGTCATAATACATTAAAACATATTATATTGTATACTATGTTTGTTTTACCACATACACCAAATGAGTAACTATTATTTAATTAACTACCAAATGACATCTACCCTCCTTTTGTATTATTAAATAAATATTCTAACTCTTTTTACTACAACAATTTTAGAATGGAGGATCTAACATTTCCAACTTTTTATTGGAGTACACATACATCAATATAAAATAATTGTTGACTTATATTTAATTTAACAAAAATGTATTTTTAAGACCTCAGTTATATTTATTTTTTACACTTTTAACAAAAATGTACATTATTTTCTTTAAAGGTTTTAAATTCTAATACTCAACCCGTTGGACTAGAAAAATAAAGATAGAAATAAATGCAATCTAAAGTTGGTCAGACAAACAATATTTTAGAGAAAGTAAGTATTTTTGTTCTTTGGTATCATAGGTTATGATCATGAACGGAATAATTTTCTCACCAATAATTCAAAGAACATAGTTTTCTTGGTATATGCTTTCAATTATTATTTTATTTTATACATTTTTGTTCGCAGTAGATAAGTTCTAAATTTGAATTGGTTTCTCGATTAAAAAGCATATATTTTAAACTGAATTAAGACAATAATATTTTTTTGTTTAGCTTATGCATGGGTTTGAATTAAGTGCAGGAATTTTCATGGCATTGCAAGAAAAATTGATAGCTTGTGGACCAACACTCACTATAGTAGGAATGGTGTTGAAGTTTATTGCTGGACCAGCTGCCATGGCCATTGGCTCCATTGCTATGGGTTTGCATGGTGACGTTCTTCGTGTTGCTATAATTCAGGTTTAATTTTCTAACCACTCTAATTAATATTTATTTATATGCATGTGTATGATAATAAATTGATTTTTAAGACTTTGATATTACATTATTTTAGTATGGTTGAAAATCTACCCAACTTTAGATAAGACTCAAATTGACCCTTCAAAGGTTTATGAACATAAAGGGTTTAATTTCTAACATAAAGGTTATGAACTTTGGATAAAACTCAAATTGACCCTTCAAAATTACAAACATAAGGAGTCAATTTGAACATATTCCTTCAAAGATTATAAAACTTTGGAGAAGACTCAAATTAAAAGGTCAATTTTGGTCTAGGCCAAGCTTGACTCAACCCAATCGACAACTCCAACTACGACACAATACTTTTGCATGCTTAACTTCAACAACCAACTACGGCTCTAGCAACCACACCTCCAATGACCAATCTTGGGGCAAACTATGACTAACCACCTAGGACGACATTTTATGAAACTATGTTCGATGATCAACTCCAAGGACCTTCGTTAATACCACTACATTCACTTCGAGCACATCTCTTTGTTTTCACTTTTTAGTAGAAGTGTATGAAAATGAATTTAAAAAACTTCAAGCATACGTTAGTTAACAGTATTGATTGTAGGGACTAAATTCAAGCACAATAGAAGTATAAATTAGCTAAAACAAATTTTTACTTATTTCAAAATTCTTATGACATTAATTTTAATGCAATTTTTAATATCTTTGTAGGCAGCAGTACCACAATCCATAACATCATTTATTTATGCAAAAGAGTATGGATTGCATGCAGATGTTCTTAGCACAGCGTAAGTTTCACTAATACCATTATTTAATGATAACAATTTTTAAAGAAATTTTTTTTTTGTTGATTATAAATCTTATTATTAGTATAAATCTGAGTTGATTTCATGATTAATGAATTTGTTAATTAAATTGTGGCCCTTTTTGTTTGAAATGCAGGGTGATTTTTGGGGCAATAGTTTCTCTTCCTGTGTTGGTTGCTTATTATGCAGCTTTGGAGTTTATTGTACATTGA

mRNA sequence

GTGCTCTAAAATCCAATAATAAGAAGTGTTGGGTATATTGTGGCCATGATCGGTTGGCAAGATGTTTATAAGGTGGTTGCCGCCATGGCTCCATTGTACTTTGCTTTGATCTTAGGCTATGGCTCTGTGAAATGGTGGAAAATCTTTAGCACTCAACAGTGCGACGCCATTAACAAATTAGTTTGCTATTTCACTCTCCCTCTCTTCACCTTCGACTTCACTTCCCATATCGACCCTTTTCATCTCAACTTCCCTTTCATCGCCGCCGACGCCATCGGAAAACTTATCATCGTTCTCGTCTTGGCCTTTTGGGCCAAGTGTACTACCAAAGGTAGTTATTGTTGGTCCATCACTAGCTTCTCTCTCTCTACGCTCACCAACGCCCTCGTCATTGGTGTTCCTCTCGCTAAGGTCATGTACGGTCAAATGGCTGTCGATCTCGTTGGTTCGGTGGTTCAAGCAATTGTTTGGTTGACTATACTATTGTTTGTGTTGGAACTTCGACGAACGGGGTTGGATTTGGTCGCTGCTGAAGCTTCTTCGGGGGTTGTGAGTGAAGAGAAAACTGTTGAAGTGGGTGGAGAGGGAGAAAAGGATTTAGAGGGAGAGGGCATGGAGGTTGAGGAAATGAGAAGATCAAGTAAGAGTCATAGGCCTTCTTTGAAGCCTTTGATGAAGAAAGTTTGGGTGAAGATGGCTGGGAATCCCAACTCATATGCTTGTACCATTGGCTTTGCTTGGGCATTTGTTGCAAAAAGGTGGCATGTTGAAATGCCAAGCATTATGGAGGGGTCAATTTTAATAATGTCAAAAGCTGGGATTGGCACTGCCATGTTCAACATGGGAATTTTCATGGCATTGCAAGAAAAATTGATAGCTTGTGGACCAACACTCACTATAGTAGGAATGGTGTTGAAGTTTATTGCTGGACCAGCTGCCATGGCCATTGGCTCCATTGCTATGGGTTTGCATGGTGACGTTCTTCGTGTTGCTATAATTCAGGCAGCAGTACCACAATCCATAACATCATTTATTTATGCAAAAGAGTATGGATTGCATGCAGATGTTCTTAGCACAGCGGTGATTTTTGGGGCAATAGTTTCTCTTCCTGTGTTGGTTGCTTATTATGCAGCTTTGGAGTTTATTGTACATTGA

Coding sequence (CDS)

ATGATCGGTTGGCAAGATGTTTATAAGGTGGTTGCCGCCATGGCTCCATTGTACTTTGCTTTGATCTTAGGCTATGGCTCTGTGAAATGGTGGAAAATCTTTAGCACTCAACAGTGCGACGCCATTAACAAATTAGTTTGCTATTTCACTCTCCCTCTCTTCACCTTCGACTTCACTTCCCATATCGACCCTTTTCATCTCAACTTCCCTTTCATCGCCGCCGACGCCATCGGAAAACTTATCATCGTTCTCGTCTTGGCCTTTTGGGCCAAGTGTACTACCAAAGGTAGTTATTGTTGGTCCATCACTAGCTTCTCTCTCTCTACGCTCACCAACGCCCTCGTCATTGGTGTTCCTCTCGCTAAGGTCATGTACGGTCAAATGGCTGTCGATCTCGTTGGTTCGGTGGTTCAAGCAATTGTTTGGTTGACTATACTATTGTTTGTGTTGGAACTTCGACGAACGGGGTTGGATTTGGTCGCTGCTGAAGCTTCTTCGGGGGTTGTGAGTGAAGAGAAAACTGTTGAAGTGGGTGGAGAGGGAGAAAAGGATTTAGAGGGAGAGGGCATGGAGGTTGAGGAAATGAGAAGATCAAGTAAGAGTCATAGGCCTTCTTTGAAGCCTTTGATGAAGAAAGTTTGGGTGAAGATGGCTGGGAATCCCAACTCATATGCTTGTACCATTGGCTTTGCTTGGGCATTTGTTGCAAAAAGGTGGCATGTTGAAATGCCAAGCATTATGGAGGGGTCAATTTTAATAATGTCAAAAGCTGGGATTGGCACTGCCATGTTCAACATGGGAATTTTCATGGCATTGCAAGAAAAATTGATAGCTTGTGGACCAACACTCACTATAGTAGGAATGGTGTTGAAGTTTATTGCTGGACCAGCTGCCATGGCCATTGGCTCCATTGCTATGGGTTTGCATGGTGACGTTCTTCGTGTTGCTATAATTCAGGCAGCAGTACCACAATCCATAACATCATTTATTTATGCAAAAGAGTATGGATTGCATGCAGATGTTCTTAGCACAGCGGTGATTTTTGGGGCAATAGTTTCTCTTCCTGTGTTGGTTGCTTATTATGCAGCTTTGGAGTTTATTGTACATTGA

Protein sequence

MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTSHIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPLAKVMYGQMAVDLVGSVVQAIVWLTILLFVLELRRTGLDLVAAEASSGVVSEEKTVEVGGEGEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVAKRWHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGPAAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPVLVAYYAALEFIVH*
Homology
BLAST of CSPI02G07160 vs. ExPASy Swiss-Prot
Match: Q9FFD0 (Auxin efflux carrier component 5 OS=Arabidopsis thaliana OX=3702 GN=PIN5 PE=2 SV=2)

HSP 1 Score: 473.8 bits (1218), Expect = 1.8e-132
Identity = 252/369 (68.29%), Postives = 290/369 (78.59%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           MI   DVYKV+ AM PLY ALILGYGSVKWW IF+  QCDAIN+LVCYFTLPLFT +FT+
Sbjct: 1   MINCGDVYKVIEAMVPLYVALILGYGSVKWWHIFTRDQCDAINRLVCYFTLPLFTIEFTA 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120
           H+DPF++N+ FIAAD + K+IIV VLA WAK + KGSYCWSITSFSL TLTN+LV+GVPL
Sbjct: 61  HVDPFNMNYRFIAADVLSKVIIVTVLALWAKYSNKGSYCWSITSFSLCTLTNSLVVGVPL 120

Query: 121 AKVMYGQMAVDLV--GSVVQAIVWLTILLFVLELRRTGLDLVAAEASSGVVSEEKTVEVG 180
           AK MYGQ AVDLV   SV QAIVWLT+LLFVLE R+ G        SS  +S+ +   + 
Sbjct: 121 AKAMYGQQAVDLVVQSSVFQAIVWLTLLLFVLEFRKAGF-------SSNNISDVQVDNIN 180

Query: 181 GEGEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVAKR 240
            E  K            R +      S   +M  VW+K+A NPN Y+C +G AWAF++ R
Sbjct: 181 IESGK------------RETVVVGEKSFLEVMSLVWLKLATNPNCYSCILGIAWAFISNR 240

Query: 241 WHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGPAA 300
           WH+E+P I+EGSILIMSKAG GTAMFNMGIFMALQEKLI CG +LT++GMVLKFIAGPAA
Sbjct: 241 WHLELPGILEGSILIMSKAGTGTAMFNMGIFMALQEKLIVCGTSLTVMGMVLKFIAGPAA 300

Query: 301 MAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPVLV 360
           MAIGSI +GLHGDVLRVAIIQAA+PQSITSFI+AKEYGLHADVLSTAVIFG +VSLPVLV
Sbjct: 301 MAIGSIVLGLHGDVLRVAIIQAALPQSITSFIFAKEYGLHADVLSTAVIFGMLVSLPVLV 350

Query: 361 AYYAALEFI 368
           AYYAALEFI
Sbjct: 361 AYYAALEFI 350

BLAST of CSPI02G07160 vs. ExPASy Swiss-Prot
Match: Q5JLM1 (Probable auxin efflux carrier component 5a OS=Oryza sativa subsp. japonica OX=39947 GN=PIN5A PE=2 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 3.3e-110
Identity = 219/373 (58.71%), Postives = 272/373 (72.92%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           MIGW DVYKVVAA  PLYFAL LGYGSV+WW+IF+ +QCDA+N+LV +F LP FTF+FT 
Sbjct: 1   MIGWGDVYKVVAATVPLYFALFLGYGSVRWWRIFTREQCDAVNRLVAFFALPFFTFEFTL 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKG--SYCWSITSFSLSTLTNALVIGV 120
           H DPF +N+  +AAD I K +IV V+  WA+  +KG  +  WSITSFSLSTLTN+LV+GV
Sbjct: 61  HTDPFQVNYRAVAADVISKAVIVAVIGAWARFMSKGGCAVSWSITSFSLSTLTNSLVVGV 120

Query: 121 PLAKVMYGQMAVDLVG--SVVQAIVWLTILLFVLELRRTGLDLVAAEASSGVVSEEKTVE 180
           P+A+ MYG+ A  LV   SV QAIVWLT+LLFVLE+R+  + +    A           E
Sbjct: 121 PMARAMYGEWAQQLVVQLSVFQAIVWLTLLLFVLEVRKAAIGMYVDGA-----------E 180

Query: 181 VGGEGEKDLEGEGMEVE--EMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAF 240
                 KD+E  G       +  ++ + +PSL  L+K V  K+A NPN+YA  +G  WA 
Sbjct: 181 AAAAAGKDVEAAGAAAAAGTVVVAAAAGKPSLWALVKVVAHKLARNPNTYASFVGITWAC 240

Query: 241 VAKRWHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIA 300
           +A R H+ +PS  EGS+LIMSK+G G AMF+MG+FMA QEK+IACG +   +G+VLKF  
Sbjct: 241 LANRLHIALPSAFEGSVLIMSKSGTGMAMFSMGLFMAQQEKIIACGTSFAALGLVLKFAL 300

Query: 301 GPAAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSL 360
           GPAAMAIGSIA+GL GDVLRVAIIQAA+PQSITSFI+AKEYGLHADVLSTAVIFG +VSL
Sbjct: 301 GPAAMAIGSIAVGLRGDVLRVAIIQAALPQSITSFIFAKEYGLHADVLSTAVIFGMLVSL 360

Query: 361 PVLVAYYAALEFI 368
           P+LV +Y  LE I
Sbjct: 361 PLLVGFYIVLELI 362

BLAST of CSPI02G07160 vs. ExPASy Swiss-Prot
Match: D5A7J3 (Probable auxin efflux carrier component 5b OS=Oryza sativa subsp. japonica OX=39947 GN=PIN5B PE=2 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 2.5e-102
Identity = 203/382 (53.14%), Postives = 261/382 (68.32%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           MIGW DVYKVVAAMAPLYFAL LGYGSV+WW++F+  QCDA+N+LV  F +P F FDF +
Sbjct: 1   MIGWGDVYKVVAAMAPLYFALGLGYGSVRWWRLFTADQCDAVNRLVACFAVPFFAFDFAA 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTK-------------GSYCWSITSFSL 120
            IDPF L++  +AADA+ KL + L LA  A   +              G + W IT FSL
Sbjct: 61  RIDPFALSYRVLAADALSKLAVALALAACAAAASTRCCGSGGGKRGGGGGFSWCITGFSL 120

Query: 121 STLTNALVIGVPLAKVMYGQMAVDLVG--SVVQAIVWLTILLFVLELRRTGLDLVAAEAS 180
           +TL N LV+GVPL   MYG+ A DL+   SVVQ IV+  +LL   E+RR      AA   
Sbjct: 121 ATLNNTLVVGVPLLDAMYGKWARDLIVQISVVQTIVYFPLLLLAFEVRR-ATTAAAAPPP 180

Query: 181 SGVVSEEKTVEVGGEGEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYA 240
               +++  VE G                   ++ + R SL PL++ VW+K+A NPN YA
Sbjct: 181 PPTGTDDDDVEDGAAA--------------AATAAAARRSLWPLVRAVWLKVARNPNVYA 240

Query: 241 CTIGFAWAFVAKRWHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTI 300
             +G AWA V  RWHVE PSI+EGS+LIMSK G+G +MF+MG+FMALQ+K+I CG  LT+
Sbjct: 241 GVLGVAWACVTNRWHVETPSIIEGSVLIMSKTGVGLSMFSMGLFMALQDKIIVCGAGLTV 300

Query: 301 VGMVLKFIAGPAAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTA 360
           +GM L+F+AGPAA A+G+ A+GL GD+LR+AIIQAA+PQSIT+F++AKEYGLHA++LSTA
Sbjct: 301 LGMALRFVAGPAATAVGAFALGLRGDLLRLAIIQAALPQSITTFVFAKEYGLHAEILSTA 360

Query: 361 VIFGAIVSLPVLVAYYAALEFI 368
           VIFG + SLPVL+ YY  L FI
Sbjct: 361 VIFGTLASLPVLIVYYIVLGFI 367

BLAST of CSPI02G07160 vs. ExPASy Swiss-Prot
Match: Q6ZIB5 (Probable auxin efflux carrier component 5c OS=Oryza sativa subsp. japonica OX=39947 GN=PIN5C PE=2 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 6.7e-95
Identity = 190/375 (50.67%), Postives = 247/375 (65.87%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           MIGW DVYKVV AMAPLYFAL LGYGSV+WW+ F+ +QC AIN +V YF++P FTFDF  
Sbjct: 1   MIGWGDVYKVVGAMAPLYFALGLGYGSVRWWRFFTAEQCAAINTMVVYFSMPFFTFDFVV 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAK------CTTKGSYCWSITSFSLSTLTNAL 120
             DPF +N+  IAADA+ K I +  +A WA+          G+  WSIT FSL+ L N L
Sbjct: 61  RTDPFAMNYRVIAADAVSKAIAIAAMAAWARTRCGCAAAKAGAQSWSITGFSLAALNNTL 120

Query: 121 VIGVPLAKVMYGQMAVDLVG--SVVQAIVWLTILLFVLELRRTGLDLVAAEASSGVVSEE 180
           V+GVPL   MYG+ A DLV   +VVQ++VW  +LL   ELR+  +         GV    
Sbjct: 121 VVGVPLLDAMYGRWAQDLVVQIAVVQSMVWFPLLLMAFELRKAWV----VGGGGGVGPAV 180

Query: 181 KTVEVGGEGEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAW 240
            +     E + D+E  G  V           P      + V +K+A NPN YA  +G  W
Sbjct: 181 MSSSSPPEKQSDVEMNGAVVAAPGGGGGVRLP-FWATARTVGLKLARNPNVYASVLGVVW 240

Query: 241 AFVAKRWHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKF 300
           A +A RWH+ +P I+ GS+ +MS+ G G +MF+MG+FM  QE++IACG  LT +GM L+F
Sbjct: 241 ACIAYRWHLSLPGIVTGSLQVMSRTGTGMSMFSMGLFMGQQERVIACGAGLTALGMALRF 300

Query: 301 IAGPAAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIV 360
           +AGP A  +G+ A+GL GDVL +AIIQAA+PQSI SF++AKEYGLHADVLSTAVIFG ++
Sbjct: 301 VAGPLATLVGAAALGLRGDVLHLAIIQAALPQSIASFVFAKEYGLHADVLSTAVIFGTLI 360

Query: 361 SLPVLVAYYAALEFI 368
           SLP+L+AYYA L F+
Sbjct: 361 SLPILIAYYAVLGFV 370

BLAST of CSPI02G07160 vs. ExPASy Swiss-Prot
Match: Q0JJV0 (Probable auxin efflux carrier component 8 OS=Oryza sativa subsp. japonica OX=39947 GN=PIN8 PE=2 SV=2)

HSP 1 Score: 250.0 bits (637), Expect = 4.2e-65
Identity = 139/366 (37.98%), Postives = 204/366 (55.74%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           M+ W+D+Y V+ A  PLY A+IL Y S+KWWK+F+ +QC  INK V  F++PL +F   S
Sbjct: 1   MVSWKDIYLVLEATVPLYVAMILAYLSIKWWKLFTPEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120
             DP+ +N   I +D + K + +L  A  +K      + W IT FSLSTL N L++G+PL
Sbjct: 61  TTDPYDMNIKLIYSDILQKSLALLGFAAISKACCAEKFDWLITGFSLSTLPNTLIVGIPL 120

Query: 121 AKVMYGQMAVDLVGSVV--QAIVWLTILLFVLELRRTGLDLVAAEASSGVVSEEKTVEVG 180
            K MYG+ A  L+  +V  Q+++W T+LLF+ ELR       AA   +   S E      
Sbjct: 121 LKGMYGEQAGKLLSQIVVLQSLIWYTLLLFLFELR-------AANGMATTTSSE------ 180

Query: 181 GEGEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVAKR 240
                                                           T G  WA V  R
Sbjct: 181 ------------------------------------------------TTGLIWALVGFR 240

Query: 241 WHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGPAA 300
           WH+ +P I+  SI ++S  G+G AMF++G+F ALQ K+IACG    ++ + ++F  GPA 
Sbjct: 241 WHIRLPLIVSNSIRMLSDGGLGMAMFSLGLFTALQTKIIACGAKRMLLALAIRFFLGPAL 300

Query: 301 MAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPVLV 360
           M + S A+G+ G +L++AI+QAA+PQ I  F++AKEY + AD+LSTA+I G +V++PV +
Sbjct: 301 MGMSSYAIGMRGVLLKIAIVQAALPQGIVPFVFAKEYNVQADILSTAIIVGMMVAVPVAL 305

Query: 361 AYYAAL 365
           AYY A+
Sbjct: 361 AYYFAM 305

BLAST of CSPI02G07160 vs. ExPASy TrEMBL
Match: A0A0A0LH23 (Auxin efflux carrier component OS=Cucumis sativus OX=3659 GN=Csa_2G074170 PE=3 SV=1)

HSP 1 Score: 708.8 bits (1828), Expect = 1.2e-200
Identity = 369/369 (100.00%), Postives = 369/369 (100.00%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS
Sbjct: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120
           HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL
Sbjct: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120

Query: 121 AKVMYGQMAVDLVGSVVQAIVWLTILLFVLELRRTGLDLVAAEASSGVVSEEKTVEVGGE 180
           AKVMYGQMAVDLVGSVVQAIVWLTILLFVLELRRTGLDLVAAEASSGVVSEEKTVEVGGE
Sbjct: 121 AKVMYGQMAVDLVGSVVQAIVWLTILLFVLELRRTGLDLVAAEASSGVVSEEKTVEVGGE 180

Query: 181 GEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVAKRWH 240
           GEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVAKRWH
Sbjct: 181 GEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVAKRWH 240

Query: 241 VEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGPAAMA 300
           VEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGPAAMA
Sbjct: 241 VEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGPAAMA 300

Query: 301 IGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPVLVAY 360
           IGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPVLVAY
Sbjct: 301 IGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPVLVAY 360

Query: 361 YAALEFIVH 370
           YAALEFIVH
Sbjct: 361 YAALEFIVH 369

BLAST of CSPI02G07160 vs. ExPASy TrEMBL
Match: A0A6J1D820 (Auxin efflux carrier component OS=Momordica charantia OX=3673 GN=LOC111018220 PE=3 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 1.0e-170
Identity = 320/371 (86.25%), Postives = 342/371 (92.18%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           MIGW+D YKVVAAMAPLYFAL LGYGSV+WWKIFST+QCDAINKLVCYFTLPLFTFDFTS
Sbjct: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120
           HIDPFHLNFPFIAADAI KL+IV+VLAFWA+CT KGSYCWSITSFSLSTLTN+LV+GVPL
Sbjct: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL 120

Query: 121 AKVMYGQMAVDLV--GSVVQAIVWLTILLFVLELRRTGLDLVAAEASS--GVVSEEKTVE 180
           AK+MYGQMAVDLV  GSVVQAIVWLTILLFVLELRRT LDLVA EASS  G     K ++
Sbjct: 121 AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKALQ 180

Query: 181 VGGEGEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVA 240
           +  EG K+LEGE MEVE  R +S SHR S KPLMKKVW+K+A NPNSYAC IGFAWAFVA
Sbjct: 181 MVEEGRKNLEGEAMEVEG-RETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFVA 240

Query: 241 KRWHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGP 300
           KRWH+EMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACG +LTI+GMVLKFIAGP
Sbjct: 241 KRWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAGP 300

Query: 301 AAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPV 360
           AAMAIGS+AMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHA+VLSTAVIFGAIVSLPV
Sbjct: 301 AAMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLPV 360

Query: 361 LVAYYAALEFI 368
           LVAYYAALEF+
Sbjct: 361 LVAYYAALEFV 370

BLAST of CSPI02G07160 vs. ExPASy TrEMBL
Match: A0A6J1FBZ5 (Auxin efflux carrier component OS=Cucurbita moschata OX=3662 GN=LOC111444027 PE=3 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 4.3e-169
Identity = 314/373 (84.18%), Postives = 341/373 (91.42%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           MIGW+DV+KV++AMAPLYFAL+LGYGSVKWWKIFST+QCDAINKLVCYFTLPLFTF+FTS
Sbjct: 1   MIGWEDVFKVLSAMAPLYFALVLGYGSVKWWKIFSTEQCDAINKLVCYFTLPLFTFEFTS 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120
           HIDPFHLNFPFIAADAI KL+IVLVLAFWAKC++KGSYCWSITSFSLSTLTNALVIGVPL
Sbjct: 61  HIDPFHLNFPFIAADAISKLVIVLVLAFWAKCSSKGSYCWSITSFSLSTLTNALVIGVPL 120

Query: 121 AKVMYGQMAVDLV--GSVVQAIVWLTILLFVLELRRTGLDLVAAEASSGVVSE--EKTVE 180
           AKVMYGQMAVDLV  GSV+QAI+WLT+LLFVLELRRTGL+LVA+  SS    +   K  +
Sbjct: 121 AKVMYGQMAVDLVVQGSVIQAIIWLTMLLFVLELRRTGLNLVASRESSSSDDDGHGKATQ 180

Query: 181 VGGEGEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVA 240
           + GEG KD+EG  MEVEE  R S SH+PS K LMKKVW+K+  NPNSYAC IG AWAFVA
Sbjct: 181 MVGEGGKDVEGGAMEVEE--RRSNSHKPSFKLLMKKVWLKLVANPNSYACIIGLAWAFVA 240

Query: 241 KRWHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGP 300
           KRWH+EMP IMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACG TLTI+GMVLKFIAGP
Sbjct: 241 KRWHIEMPDIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGATLTIIGMVLKFIAGP 300

Query: 301 AAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPV 360
           AAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLH +V STAVIFGAI+SLP+
Sbjct: 301 AAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHPEVFSTAVIFGAIISLPI 360

Query: 361 LVAYYAALEFIVH 370
           LVAYYAALEFIVH
Sbjct: 361 LVAYYAALEFIVH 371

BLAST of CSPI02G07160 vs. ExPASy TrEMBL
Match: A0A6J1HQQ5 (Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111465776 PE=3 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 9.5e-169
Identity = 313/373 (83.91%), Postives = 341/373 (91.42%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           MIGW+DV+KV++AMAPLYFAL+LGYGSVKWWKIFST+QCDAINKLVCYFTLPLFTFDFTS
Sbjct: 1   MIGWEDVFKVLSAMAPLYFALVLGYGSVKWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120
           HIDPFHLNF FIAADAI KL+IVLVLAFWAKC++KGSYCWSITSFSLSTLTNALVIGVPL
Sbjct: 61  HIDPFHLNFHFIAADAISKLVIVLVLAFWAKCSSKGSYCWSITSFSLSTLTNALVIGVPL 120

Query: 121 AKVMYGQMAVDLV--GSVVQAIVWLTILLFVLELRRTGLDLVAAEASSGVVSEE--KTVE 180
           AKVMYGQMAVDLV  GSV+QAI+WLT+LLFVLELRRTGL+LVA+  S     +E  K ++
Sbjct: 121 AKVMYGQMAVDLVVQGSVIQAIIWLTMLLFVLELRRTGLNLVASRESLSSDDDEHGKAIQ 180

Query: 181 VGGEGEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVA 240
           + GEG KD+EG  MEVEE  R S SH+PS K LMKKVW+K+  NPNSYAC IG AWAFVA
Sbjct: 181 MVGEGGKDVEGGAMEVEE--RRSNSHKPSFKLLMKKVWLKLVANPNSYACIIGLAWAFVA 240

Query: 241 KRWHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGP 300
           KRWH+EMP +MEGSILIMSKAGIGTAMFNMGIFMALQEKLIACG TLTI+GMVLKFIAGP
Sbjct: 241 KRWHIEMPDLMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGATLTIIGMVLKFIAGP 300

Query: 301 AAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPV 360
           AAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLH +V STAVIFGAI+SLP+
Sbjct: 301 AAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHPEVFSTAVIFGAIISLPI 360

Query: 361 LVAYYAALEFIVH 370
           LVAYYAALEFIVH
Sbjct: 361 LVAYYAALEFIVH 371

BLAST of CSPI02G07160 vs. ExPASy TrEMBL
Match: A0A2C9VF50 (Auxin efflux carrier component OS=Manihot esculenta OX=3983 GN=MANES_08G104400 PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.4e-140
Identity = 269/370 (72.70%), Postives = 307/370 (82.97%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           MIGW+DVYKVV AM PLY AL+LGYGSV+WWKIF+ +Q  A+N+ VCYFTLPLFTF+FT+
Sbjct: 1   MIGWEDVYKVVVAMVPLYVALVLGYGSVRWWKIFTPEQSGAVNRFVCYFTLPLFTFEFTA 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120
           H+DPF++N+ FI ADAI K IIV+VLAFWAK ++KGSY WSITSFSL TLTN+LV+GVPL
Sbjct: 61  HVDPFNMNYLFIGADAISKFIIVVVLAFWAKFSSKGSYTWSITSFSLCTLTNSLVVGVPL 120

Query: 121 AKVMYGQMAVDLV--GSVVQAIVWLTILLFVLELRRTGLDLVAAEASSGVVSEEKTVEVG 180
           AK MYGQMAVDLV   SV+QAI+WLTILLFVLE+RRTGLD+              +    
Sbjct: 121 AKAMYGQMAVDLVVQSSVIQAIIWLTILLFVLEIRRTGLDI--------------SSNTN 180

Query: 181 GEG-EKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVAK 240
           GE  +KDLEG    V+    S+K   PS   LMK VW+KMA NPNSYAC IG  WAF+AK
Sbjct: 181 GENLDKDLEGISRSVDHKMESAK---PSFWALMKVVWLKMAMNPNSYACIIGLVWAFIAK 240

Query: 241 RWHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGPA 300
           RWH EMPSIMEGSILIMS+AG GTAMF+MGIFMALQEKLIACG  LT+VGMVL+FIAGPA
Sbjct: 241 RWHFEMPSIMEGSILIMSRAGTGTAMFSMGIFMALQEKLIACGTGLTVVGMVLRFIAGPA 300

Query: 301 AMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPVL 360
           AMAIGSIA+GLHGDVLRVAIIQAA+PQSITSFI+AKEYGLHA+VLSTAVIFG IVSLPVL
Sbjct: 301 AMAIGSIAVGLHGDVLRVAIIQAALPQSITSFIFAKEYGLHAEVLSTAVIFGMIVSLPVL 353

Query: 361 VAYYAALEFI 368
           +AYYA LEF+
Sbjct: 361 IAYYAVLEFV 353

BLAST of CSPI02G07160 vs. NCBI nr
Match: XP_004152830.1 (auxin efflux carrier component 5 [Cucumis sativus] >KAE8651707.1 hypothetical protein Csa_006543 [Cucumis sativus])

HSP 1 Score: 703.7 bits (1815), Expect = 8.2e-199
Identity = 369/371 (99.46%), Postives = 369/371 (99.46%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS
Sbjct: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120
           HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL
Sbjct: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120

Query: 121 AKVMYGQMAVDLV--GSVVQAIVWLTILLFVLELRRTGLDLVAAEASSGVVSEEKTVEVG 180
           AKVMYGQMAVDLV  GSVVQAIVWLTILLFVLELRRTGLDLVAAEASSGVVSEEKTVEVG
Sbjct: 121 AKVMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTGLDLVAAEASSGVVSEEKTVEVG 180

Query: 181 GEGEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVAKR 240
           GEGEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVAKR
Sbjct: 181 GEGEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVAKR 240

Query: 241 WHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGPAA 300
           WHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGPAA
Sbjct: 241 WHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGPAA 300

Query: 301 MAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPVLV 360
           MAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPVLV
Sbjct: 301 MAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPVLV 360

Query: 361 AYYAALEFIVH 370
           AYYAALEFIVH
Sbjct: 361 AYYAALEFIVH 371

BLAST of CSPI02G07160 vs. NCBI nr
Match: XP_038889666.1 (auxin efflux carrier component 5 [Benincasa hispida])

HSP 1 Score: 657.5 bits (1695), Expect = 6.7e-185
Identity = 343/369 (92.95%), Postives = 357/369 (96.75%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           MIGW+DVYKVV+AMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS
Sbjct: 1   MIGWEDVYKVVSAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120
           HIDPFHLNFPFI ADAIGKLIIVLVLAFWAKCT+KGSYCWSITSFSLSTLTNALVIGVPL
Sbjct: 61  HIDPFHLNFPFIVADAIGKLIIVLVLAFWAKCTSKGSYCWSITSFSLSTLTNALVIGVPL 120

Query: 121 AKVMYGQMAVDLV--GSVVQAIVWLTILLFVLELRRTGLDLVAAEASSGVVSEEKTVEVG 180
           AKVMYGQMAVDLV  GSV+QAIVWLT+LLFVLELRRT LDL+AAEAS G VS+EK V++ 
Sbjct: 121 AKVMYGQMAVDLVVQGSVIQAIVWLTMLLFVLELRRTRLDLIAAEASPGFVSDEKAVQLT 180

Query: 181 GEGEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVAKR 240
           GEG KDLEGE M+VEE RRSS SHRPSLKPLMKKVW+K+AGNPNSYACTIGFAWAFVAKR
Sbjct: 181 GEGGKDLEGEAMKVEE-RRSSNSHRPSLKPLMKKVWLKLAGNPNSYACTIGFAWAFVAKR 240

Query: 241 WHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGPAA 300
           WHVEMP+IMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTI+GMVLKFIAGPAA
Sbjct: 241 WHVEMPNIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIIGMVLKFIAGPAA 300

Query: 301 MAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPVLV 360
           MAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPVLV
Sbjct: 301 MAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPVLV 360

Query: 361 AYYAALEFI 368
           AYYAALEFI
Sbjct: 361 AYYAALEFI 368

BLAST of CSPI02G07160 vs. NCBI nr
Match: XP_022149923.1 (auxin efflux carrier component 5 [Momordica charantia])

HSP 1 Score: 609.4 bits (1570), Expect = 2.1e-170
Identity = 320/371 (86.25%), Postives = 342/371 (92.18%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           MIGW+D YKVVAAMAPLYFAL LGYGSV+WWKIFST+QCDAINKLVCYFTLPLFTFDFTS
Sbjct: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120
           HIDPFHLNFPFIAADAI KL+IV+VLAFWA+CT KGSYCWSITSFSLSTLTN+LV+GVPL
Sbjct: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL 120

Query: 121 AKVMYGQMAVDLV--GSVVQAIVWLTILLFVLELRRTGLDLVAAEASS--GVVSEEKTVE 180
           AK+MYGQMAVDLV  GSVVQAIVWLTILLFVLELRRT LDLVA EASS  G     K ++
Sbjct: 121 AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKALQ 180

Query: 181 VGGEGEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVA 240
           +  EG K+LEGE MEVE  R +S SHR S KPLMKKVW+K+A NPNSYAC IGFAWAFVA
Sbjct: 181 MVEEGRKNLEGEAMEVEG-RETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFVA 240

Query: 241 KRWHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGP 300
           KRWH+EMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACG +LTI+GMVLKFIAGP
Sbjct: 241 KRWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAGP 300

Query: 301 AAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPV 360
           AAMAIGS+AMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHA+VLSTAVIFGAIVSLPV
Sbjct: 301 AAMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLPV 360

Query: 361 LVAYYAALEFI 368
           LVAYYAALEF+
Sbjct: 361 LVAYYAALEFV 370

BLAST of CSPI02G07160 vs. NCBI nr
Match: XP_023536981.1 (auxin efflux carrier component 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 604.4 bits (1557), Expect = 6.7e-169
Identity = 314/373 (84.18%), Postives = 342/373 (91.69%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           MIGW+DV+KV++AMAPLYFAL+LGYGSVKWWKIFST+QCDAINKLVCYFTLPLFTF+FTS
Sbjct: 1   MIGWEDVFKVLSAMAPLYFALVLGYGSVKWWKIFSTEQCDAINKLVCYFTLPLFTFEFTS 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120
           HIDPFHLNF FIAADAI KL+IVLVLAFWAKC++KGSYCWSITSFSLSTLTNALVIGVPL
Sbjct: 61  HIDPFHLNFHFIAADAISKLVIVLVLAFWAKCSSKGSYCWSITSFSLSTLTNALVIGVPL 120

Query: 121 AKVMYGQMAVDLV--GSVVQAIVWLTILLFVLELRRTGLDLVAAEASSGVVSE--EKTVE 180
           AKVMYGQMAVDLV  GSV+QAI+WLT+LLFVLELRRTGL+LVA+  SS    +  EK ++
Sbjct: 121 AKVMYGQMAVDLVVQGSVIQAIIWLTMLLFVLELRRTGLNLVASRESSSSDDDGHEKAIQ 180

Query: 181 VGGEGEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVA 240
           + GEG KD+EG  MEVEE  R S SH+PS K LMKKVW+K+  NPNSYAC IG AWAFVA
Sbjct: 181 MVGEGGKDVEGGAMEVEE--RRSNSHKPSFKLLMKKVWLKLVANPNSYACIIGLAWAFVA 240

Query: 241 KRWHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGP 300
           KRWH+EMP IMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACG TLTI+GMVLKFIAGP
Sbjct: 241 KRWHIEMPDIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGATLTIIGMVLKFIAGP 300

Query: 301 AAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPV 360
           AAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLH +V STAVIFGAI+SLP+
Sbjct: 301 AAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHPEVFSTAVIFGAIISLPI 360

Query: 361 LVAYYAALEFIVH 370
           LVAYYAALEFIVH
Sbjct: 361 LVAYYAALEFIVH 371

BLAST of CSPI02G07160 vs. NCBI nr
Match: XP_022937707.1 (auxin efflux carrier component 5 [Cucurbita moschata])

HSP 1 Score: 604.0 bits (1556), Expect = 8.8e-169
Identity = 314/373 (84.18%), Postives = 341/373 (91.42%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           MIGW+DV+KV++AMAPLYFAL+LGYGSVKWWKIFST+QCDAINKLVCYFTLPLFTF+FTS
Sbjct: 1   MIGWEDVFKVLSAMAPLYFALVLGYGSVKWWKIFSTEQCDAINKLVCYFTLPLFTFEFTS 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120
           HIDPFHLNFPFIAADAI KL+IVLVLAFWAKC++KGSYCWSITSFSLSTLTNALVIGVPL
Sbjct: 61  HIDPFHLNFPFIAADAISKLVIVLVLAFWAKCSSKGSYCWSITSFSLSTLTNALVIGVPL 120

Query: 121 AKVMYGQMAVDLV--GSVVQAIVWLTILLFVLELRRTGLDLVAAEASSGVVSE--EKTVE 180
           AKVMYGQMAVDLV  GSV+QAI+WLT+LLFVLELRRTGL+LVA+  SS    +   K  +
Sbjct: 121 AKVMYGQMAVDLVVQGSVIQAIIWLTMLLFVLELRRTGLNLVASRESSSSDDDGHGKATQ 180

Query: 181 VGGEGEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVA 240
           + GEG KD+EG  MEVEE  R S SH+PS K LMKKVW+K+  NPNSYAC IG AWAFVA
Sbjct: 181 MVGEGGKDVEGGAMEVEE--RRSNSHKPSFKLLMKKVWLKLVANPNSYACIIGLAWAFVA 240

Query: 241 KRWHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGP 300
           KRWH+EMP IMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACG TLTI+GMVLKFIAGP
Sbjct: 241 KRWHIEMPDIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGATLTIIGMVLKFIAGP 300

Query: 301 AAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPV 360
           AAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLH +V STAVIFGAI+SLP+
Sbjct: 301 AAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHPEVFSTAVIFGAIISLPI 360

Query: 361 LVAYYAALEFIVH 370
           LVAYYAALEFIVH
Sbjct: 361 LVAYYAALEFIVH 371

BLAST of CSPI02G07160 vs. TAIR 10
Match: AT5G16530.1 (Auxin efflux carrier family protein )

HSP 1 Score: 473.8 bits (1218), Expect = 1.3e-133
Identity = 252/369 (68.29%), Postives = 290/369 (78.59%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           MI   DVYKV+ AM PLY ALILGYGSVKWW IF+  QCDAIN+LVCYFTLPLFT +FT+
Sbjct: 1   MINCGDVYKVIEAMVPLYVALILGYGSVKWWHIFTRDQCDAINRLVCYFTLPLFTIEFTA 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120
           H+DPF++N+ FIAAD + K+IIV VLA WAK + KGSYCWSITSFSL TLTN+LV+GVPL
Sbjct: 61  HVDPFNMNYRFIAADVLSKVIIVTVLALWAKYSNKGSYCWSITSFSLCTLTNSLVVGVPL 120

Query: 121 AKVMYGQMAVDLV--GSVVQAIVWLTILLFVLELRRTGLDLVAAEASSGVVSEEKTVEVG 180
           AK MYGQ AVDLV   SV QAIVWLT+LLFVLE R+ G        SS  +S+ +   + 
Sbjct: 121 AKAMYGQQAVDLVVQSSVFQAIVWLTLLLFVLEFRKAGF-------SSNNISDVQVDNIN 180

Query: 181 GEGEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVAKR 240
            E  K            R +      S   +M  VW+K+A NPN Y+C +G AWAF++ R
Sbjct: 181 IESGK------------RETVVVGEKSFLEVMSLVWLKLATNPNCYSCILGIAWAFISNR 240

Query: 241 WHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGPAA 300
           WH+E+P I+EGSILIMSKAG GTAMFNMGIFMALQEKLI CG +LT++GMVLKFIAGPAA
Sbjct: 241 WHLELPGILEGSILIMSKAGTGTAMFNMGIFMALQEKLIVCGTSLTVMGMVLKFIAGPAA 300

Query: 301 MAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPVLV 360
           MAIGSI +GLHGDVLRVAIIQAA+PQSITSFI+AKEYGLHADVLSTAVIFG +VSLPVLV
Sbjct: 301 MAIGSIVLGLHGDVLRVAIIQAALPQSITSFIFAKEYGLHADVLSTAVIFGMLVSLPVLV 350

Query: 361 AYYAALEFI 368
           AYYAALEFI
Sbjct: 361 AYYAALEFI 350

BLAST of CSPI02G07160 vs. TAIR 10
Match: AT2G01420.1 (Auxin efflux carrier family protein )

HSP 1 Score: 250.4 bits (638), Expect = 2.3e-66
Identity = 185/610 (30.33%), Postives = 251/610 (41.15%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           MI W D+Y V+ A+ PLY A+IL YGSV+WWKIFS  QC  IN+ V  F +PL +F F S
Sbjct: 1   MITWHDLYTVLTAVVPLYVAMILAYGSVQWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120
             DP+ +NF F+AAD + K+I++++LA WA  T  GS  W IT FSLSTL N LV+G+PL
Sbjct: 61  TNDPYAMNFRFVAADTLQKIIMLVLLALWANLTKNGSLEWMITIFSLSTLPNTLVMGIPL 120

Query: 121 AKVMYGQMAVDLVGSVV--QAIVWLTILLFVLELR-----------RTGLDLVAAEASSG 180
              MYG  A  L+  VV  Q I+W T+LLF+ E R            TG  +V+ +  S 
Sbjct: 121 LIAMYGTYAGSLMVQVVVLQCIIWYTLLLFLFEYRGAKLLIMEQFPETGASIVSFKVESD 180

Query: 181 VVS--------------------------------------------------------- 240
           VVS                                                         
Sbjct: 181 VVSLDGHDFLETDAEIGNDGKLHVTVRKSNASRRSLMMTPRPSNLTGAEIYSLSSTPRGS 240

Query: 241 -------------------------------------------EEKTVEVG--------- 300
                                                      E   V+ G         
Sbjct: 241 NFNHSDFYSVMGFPGGRLSNFGPADLYSVQSSRGPTPRPSNFEENNAVKYGFYNNTNSSV 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 PAAGSYPAPNPEFSTGTGVSTKPNKIPKENQQQLQEKDSKASHDAKELHMFVWSSSASPV 360

Query: 361 ---------------------------------------------GEGEKDLEGEGMEVE 365
                                                        GEGE+++E     + 
Sbjct: 361 SDVFGGGAGDNVATEQSEQGAKEIRMVVSDQPRKSGGDDIGGLDSGEGEREIEKATAGLN 420

BLAST of CSPI02G07160 vs. TAIR 10
Match: AT2G01420.2 (Auxin efflux carrier family protein )

HSP 1 Score: 248.8 bits (634), Expect = 6.7e-66
Identity = 185/614 (30.13%), Postives = 251/614 (40.88%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           MI W D+Y V+ A+ PLY A+IL YGSV+WWKIFS  QC  IN+ V  F +PL +F F S
Sbjct: 1   MITWHDLYTVLTAVVPLYVAMILAYGSVQWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120
             DP+ +NF F+AAD + K+I++++LA WA  T  GS  W IT FSLSTL N LV+G+PL
Sbjct: 61  TNDPYAMNFRFVAADTLQKIIMLVLLALWANLTKNGSLEWMITIFSLSTLPNTLVMGIPL 120

Query: 121 AKVMYGQMAVDLVGSVV--QAIVWLTILLFVLELR-----------RTGLDLVAAEASSG 180
              MYG  A  L+  VV  Q I+W T+LLF+ E R            TG  +V+ +  S 
Sbjct: 121 LIAMYGTYAGSLMVQVVVLQCIIWYTLLLFLFEYRGAKLLIMEQFPETGASIVSFKVESD 180

Query: 181 VVS--------------------------------------------------------- 240
           VVS                                                         
Sbjct: 181 VVSLDGHDFLETDAEIGNDGKLHVTVRKSNASRRSLMMTPRPSNLTGAEIYSLSSTPRGS 240

Query: 241 -------------------------------------------EEKTVEVG--------- 300
                                                      E   V+ G         
Sbjct: 241 NFNHSDFYSVMGFPGGRLSNFGPADLYSVQSSRGPTPRPSNFEENNAVKYGFYNNTNSSV 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 PAAGSYPAPNPEFSTGTGVSTKPNKIPKENQQQLQEKDSKASHDAKELHMFVWSSSASPV 360

Query: 361 -------------------------------------------------GEGEKDLEGEG 365
                                                            GEGE+++E   
Sbjct: 361 SDVFGGGAGDNVATEQSEQGAKEIRMVVSDQPRKSNARGGGDDIGGLDSGEGEREIEKAT 420

BLAST of CSPI02G07160 vs. TAIR 10
Match: AT5G15100.1 (Auxin efflux carrier family protein )

HSP 1 Score: 240.7 bits (613), Expect = 1.8e-63
Identity = 143/372 (38.44%), Postives = 218/372 (58.60%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           MI W D+Y VV+A  PLY ++ LG+ S +  K+FS +QC  INK V  F++PL +F   S
Sbjct: 1   MISWLDIYHVVSATVPLYVSMTLGFLSARHLKLFSPEQCAGINKFVAKFSIPLLSFQIIS 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLA----FWAKCTTKGSYC-WSITSFSLSTLTNALV 120
             +PF ++   I +D + K ++V+VLA    FW     +G    W IT  S+S L N L+
Sbjct: 61  ENNPFKMSPKLILSDILQKFLVVVVLAMVLRFWHPTGGRGGKLGWVITGLSISVLPNTLI 120

Query: 121 IGVPLAKVMYGQMAVDLVGSVV--QAIVWLTILLFVLELRRTGLDLVAAEASSGVVSEEK 180
           +G+P+   +YG  A  ++  +V  Q+++W TILLF+ E     L+   A  SSG  S E 
Sbjct: 121 LGMPILSAIYGDEAASILEQIVVLQSLIWYTILLFLFE-----LNAARALPSSG-ASLEH 180

Query: 181 TVEVGGEGEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWA 240
           T     E   + E +  E EE     ++       ++ K W K+  NPN+YA  IG  WA
Sbjct: 181 TGNDQEEANIEDEPKEEEDEEEVAIVRTRSVGTMKILLKAWRKLIINPNTYATLIGIIWA 240

Query: 241 FVAKRWHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFI 300
            +  R    +P +++ SI ++S  G+G AMF++G+FMA Q  +IACG  + I+ M+LKF+
Sbjct: 241 TLHFRLGWNLPEMIDKSIHLLSDGGLGMAMFSLGLFMASQSSIIACGTKMAIITMLLKFV 300

Query: 301 AGPAAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVS 360
            GPA M   +  + L   + +VAI+QAA+PQ +  F++AKEY LH +++ST VIFG +++
Sbjct: 301 LGPALMIASAYCIRLKSTLFKVAILQAALPQGVVPFVFAKEYNLHPEIISTGVIFGMLIA 360

Query: 361 LPVLVAYYAALE 366
           LP  +AYY  L+
Sbjct: 361 LPTTLAYYFLLD 366

BLAST of CSPI02G07160 vs. TAIR 10
Match: AT1G23080.3 (Auxin efflux carrier family protein )

HSP 1 Score: 231.9 bits (590), Expect = 8.5e-61
Identity = 180/613 (29.36%), Postives = 249/613 (40.62%), Query Frame = 0

Query: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60
           MI W D+Y V+ A+ PLY A+IL YGSV+WWKIFS  QC  IN+ V  F +PL +F F S
Sbjct: 1   MITWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120
             +P+ +N  FIAAD + KLI++ +L  WA  T  GS  WSIT FSLSTL N LV+G+PL
Sbjct: 61  SNNPYAMNLRFIAADTLQKLIMLTLLIIWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 AKVMYGQMAVDLVGSVV--QAIVWLTILLFVLELR-----------RTGLDLVAAEASSG 180
              MYG+ +  L+  +V  Q I+W T+LLF+ E R            TG  +V+ +  S 
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEYRGAKILIMEQFPETGASIVSFKVESD 180

Query: 181 VVS------EEKTVEVGGEGE--------------------------------------- 240
           VVS       E   ++G +G+                                       
Sbjct: 181 VVSLDGHDFLETDAQIGDDGKLHVTVRKSNASRRSFYGGGGTNMTPRPSNLTGAEIYSLN 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 TTPRGSNFNHSDFYSMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEESCAMASSPRF 300

Query: 301 --------------------------------------------KDL------------- 360
                                                       K+L             
Sbjct: 301 GYYPGGAPGSYPAPNPEFSTGNKTGSKAPKENHHHVGKSNSNDAKELHMFVWGSNGSPVS 360

Query: 361 EGEGMEVE----------------EMRRSSKSHRPSLKP--------------------- 365
           +  G++V+                E+R     H  +  P                     
Sbjct: 361 DRAGLQVDNGANEQVGKSDQGGAKEIRMLISDHTQNAGPMNGDYGGEEESERVKEVPNGL 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FFD01.8e-13268.29Auxin efflux carrier component 5 OS=Arabidopsis thaliana OX=3702 GN=PIN5 PE=2 SV... [more]
Q5JLM13.3e-11058.71Probable auxin efflux carrier component 5a OS=Oryza sativa subsp. japonica OX=39... [more]
D5A7J32.5e-10253.14Probable auxin efflux carrier component 5b OS=Oryza sativa subsp. japonica OX=39... [more]
Q6ZIB56.7e-9550.67Probable auxin efflux carrier component 5c OS=Oryza sativa subsp. japonica OX=39... [more]
Q0JJV04.2e-6537.98Probable auxin efflux carrier component 8 OS=Oryza sativa subsp. japonica OX=399... [more]
Match NameE-valueIdentityDescription
A0A0A0LH231.2e-200100.00Auxin efflux carrier component OS=Cucumis sativus OX=3659 GN=Csa_2G074170 PE=3 S... [more]
A0A6J1D8201.0e-17086.25Auxin efflux carrier component OS=Momordica charantia OX=3673 GN=LOC111018220 PE... [more]
A0A6J1FBZ54.3e-16984.18Auxin efflux carrier component OS=Cucurbita moschata OX=3662 GN=LOC111444027 PE=... [more]
A0A6J1HQQ59.5e-16983.91Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111465776 PE=3 ... [more]
A0A2C9VF501.4e-14072.70Auxin efflux carrier component OS=Manihot esculenta OX=3983 GN=MANES_08G104400 P... [more]
Match NameE-valueIdentityDescription
XP_004152830.18.2e-19999.46auxin efflux carrier component 5 [Cucumis sativus] >KAE8651707.1 hypothetical pr... [more]
XP_038889666.16.7e-18592.95auxin efflux carrier component 5 [Benincasa hispida][more]
XP_022149923.12.1e-17086.25auxin efflux carrier component 5 [Momordica charantia][more]
XP_023536981.16.7e-16984.18auxin efflux carrier component 5 [Cucurbita pepo subsp. pepo][more]
XP_022937707.18.8e-16984.18auxin efflux carrier component 5 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G16530.11.3e-13368.29Auxin efflux carrier family protein [more]
AT2G01420.12.3e-6630.33Auxin efflux carrier family protein [more]
AT2G01420.26.7e-6630.13Auxin efflux carrier family protein [more]
AT5G15100.11.8e-6338.44Auxin efflux carrier family protein [more]
AT1G23080.38.5e-6129.36Auxin efflux carrier family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014024Auxin efflux carrier, plant typeTIGRFAMTIGR00946TIGR00946coord: 1..364
e-value: 1.2E-84
score: 282.2
IPR004776Membrane transport proteinPFAMPF03547Mem_transcoord: 10..361
e-value: 5.1E-88
score: 295.1
NoneNo IPR availablePANTHERPTHR31752AUXIN EFFLUX CARRIER COMPONENT 1B-RELATEDcoord: 1..367
NoneNo IPR availablePANTHERPTHR31752:SF2AUXIN EFFLUX CARRIER COMPONENT 5coord: 1..367

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G07160.1CSPI02G07160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010315 auxin efflux
biological_process GO:0010252 auxin homeostasis
biological_process GO:0009926 auxin polar transport
biological_process GO:0080162 intracellular auxin transport
biological_process GO:0009555 pollen development
biological_process GO:0055085 transmembrane transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0010329 auxin efflux transmembrane transporter activity