CSPI02G06590 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G06590
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	methyl-CpG-binding domain-containing protein 2-like
Location	Chr2: 5347729 .. 5353374 (+)
RNA-Seq Expression	CSPI02G06590
Synteny	CSPI02G06590

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTCAATAATTTAGAATATGACGTTGTTTTTGGACTAAAACCAATTTTCCAAATTCCATTCACGAATCAATCCACCTTCTTCTTCTTCTTCCTTCTCTTCACAACGTCTATCTGCACAATCCTTTCTCCCTCTTCTCCTTTCCCTTTTCCCGAGGCCTTACCTATCATTTCAACACCAAAATTCCCACACCCACCTTCCATTTTCCTTGGTGCTTCCGCATTTTCCATCCCCCACCTGCCTACTTCCTTGAAATCAACGCATTTCATCTCTCCTCTCTTCCTATGATTCTAAATTTGTGTAAACATCTCTTTCTTCCTCCTCTGTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTGTTTGATTCTTTTGGGTATTTGAGTCAATTTGTTGAGGGGAATGGTGTTTGGAATTGAAGAATTTGCGGGTTTGATGGTTTAGATAGATTGGATGATATTTTGGGTTTGAGTGTTTTTATTTTATTCTTTTATTTTGATGATGGGAAAGGGTGTGGATCTATGGTGGAGGTGGGTTTGTGTTATGGGTTCCGATCGTGATAAGAATTGAATGATCTCATGAGATTGGTCATCAATTTTTTGTTTTTTGCACTTTGATTATGAAGCTGGAGGGAAATTATGGTTATGCTTCTTCTCCTCCCATGGCGGTCGATCTTGCTGTTTTGTTTTCTTTATTGTTGTGGGTTTTTGTTTTAAGATGATCTTGCTGGCTTTACCTGTATTGGGTTCAAATATTTCTTCTAGTTGCTCGTGATGATCTCTTCACTGCATTGTTGTTTAGAGCTTGGAGTTATGTGCTAAACCTTCTCGCCTCTTTTACACTTTGATCATCGCTTAAGTTTGTTGCGTTTACCAGTTTTTTCTTAATGTTATATATATACATATATAGAAAGTTTTTGTTTGTAGCAAGGAAATTCAGAGGCTTTGATTATCTGTAATATCGTGCACTATCCTTATGTCTTTCTTGGATGAAACGCCGAGTTTTATTGTGATGAATCAAAAAATTCAAGTAACAGGAGAAATTAACGTAGAAGACCCAAAATGAAAACCTTCAATATAGATTCAATATAGGGGTTGCAAAGCGAGACATACAGATGTGTTAGAGATCATGAAATTTGTGGTTAGAATCCCCCTACAACTAATGTACTGAAAAAATCGTAGATTCAATAAAGATTCCGATCAATAAGAATAAGGATGAAAGAATAACTCCAAAACACTTTATAAATAAACACCCATATTGAGGTGTTGAACTTAGCTCTATAAGTTCTCTTAAGAAAATGGAAATGCACCTTATGAATATTTTGAGTTTAATTTTGAATTATTTCTGTTCATCTCCCTGTTCCTTTACTTTAACTATTTGGTAGTAACAACTCTCAACAAACTTTTACTAACTAATATACTGACCAGCTAACTCAATGTCCAACAACTAACTCCCTTCTCTAACTACTTAGTCCTGAGATCTAAATTCTTTTTGGTCTTCATCATTTTTTCTTAGCTTACTGGTGTTGTTCTCTTCATAAAGTCCTTTTTACCCTTGCAATTAGGAAGAATATCCTGTTTGTGGTATCTTTTCTTCTCAAGGATTTCATCTTCGCTCTGAGTTTTGACTTGTAAAGATGTGATTGTCTTGTTATTGAATATCAGAATTTGGGATTATGTTTCAATTGGAACACTATTTTCTCTCTTAGTAATCATTCTTGTTCGATAGATGCCAGTAAATGTTTCATTTGGTAATCTCTTTTGGCATTGGGAGTGGGAGTGCTGTCATCCCCTTCCCTCTTAGTAATAAAATTTACCTTTTCGTTTCTAATAAAAAAGAATGTATACTGAGATTACATTGCTTCTCTTTGGCATCTGAGTTTTTTCTGTAATTATTTGTTGGGATTTATTTTGTTTGACTAGAGACCGTTATTTTAGGATGCTCCTTTTTCGTGGGCTAGGTTTATTGTATGCCCTTGTGTTTTTTCATCTTTTGTCTACGAAATTCGATCTTTTATCTCAATAAAGCAAACTGAGATTATAGTTTGAATCTGTTTTCTTTGTCTATGGCAGGATTTTGGATCACAGTATGATGTGGTTAAACCGCTGATAAAATTACCATTATGGTTGGTGGGGAAGAAAATGACTTGAATGAGCCAAGTTTGTCAAACGACCAAGAAACTCCACGTCAAGATGTTATAGCTGTTTCTTCCTCTTCATCCTCTTTGTCATTTTCTTCGTCTGAGAACGAGTTACACGAGACTAGGAATGAGAATGTCCAATCTCAATCTAATGACAATCCCTCTCGTGAACTGGTTATATATGATCCGTCTGCTGATGATGGACATGCAAATGATCCTGTATCCACACCTGTAAAGCAGCAGCCTTCACCAGTTCTTGGACACTTGACTCCCAATTCTTCCAGAGTCTTACCATCGGTAGGGGCCTTTACTGTCCAATGTGCAGGCTGTTTTAAATGGAGGCTCATTCCAACGAAGGAAAAATACGAGGAAATACGAGAACATCTGTTAGAGCAACCTTTTTACTGTGAAACTGCTCGTGGGTGGCGGCCTGATATCTCCTGTGATGATCCTGCTGACATTACTCAAGATGGTAGTAGGCTCTGGGCAATTGACAAGCCGAATATAGCACAGCCCCCTCCTGGTTGGCAAAGGCTATTGCACATCAGAGGTGAAGGAAGCACCAAGTTTGCTGATGTGTATGTACTTTAAATTCTGTATTATCGCTACTTTTTTTTTTTTTTTTGGTTTTAGACATATCTGTCACCAGTTTGCCTCGGACTGCTACCATATCTCTCTAAAGTTCTTATTCTAGTTTTTCTTGGTTTATTGTTATGAATTATACTAAAAGAACAAGTCCTATAGTTAAATATCTTTGTGTTTGCTTAAAGTTTAAATGTCTTCTTTAGCATCTGTAACGGCCCGAGTTTAAGAGGGGGACATGAATGAACCAGTATTACATCACGGATCCTGAGGACATAGAAGTAAGGTTAAGAAAGACATAAAAGAATTGAAAGTTACTAACTATACCAACAAGGTCCACCTTCCTTTTCGGTGGCTCGATCATAGGAATTCTAATACTAAACATGCTTAGCTTAGAACAATTCTATGTTGGGTGACTTCCTAGGATGCATGTGAGTGATGACAAAACATGCTAAAAAGACTCGTGTTAGTTTGTAGAGAACTTTATCTCTAATAAGCTTTTAAGACAAGTAAGGATGATGTTGTCACGTTGCATGGGATGTAGGAGAATATCAGGGATCCGAGTTTCGAATCCTAGGCCTGGGGTGTTATAGAATCATTTTTCCTTTTATGGCTTCAGTTAGCCACAAATCAGCATTATTATCGATAACATTGTCATTGCTGTTGTCAAAGATGTGATGGTTACTATTCTTTTATTAAGATGTCAAATTTTTTCCTTCTCATAATTACATTATCACTTGTACAATGTACTCATTGTTATTACTTTGAGATTGTGTCCAAACCAGGGAAATACTAACCATTTGATTCTCTACTAAAAGATTTTCGCATCTTCAAAATATTTTGATAGGATGAATGATTTTAATTGAACGGGTTAACCAGGCCCAAATTAATGGAAATACTCATTGTTGTAATGAAGCTGAAAGGTCGTTTGGGTTGTAATTTTTCATTTCTATAATCATTAAGATCTTATTAAATTTGATATTTTACTGCTATCAGGTATTACACTGCTCCATCAGGCAAGAAGCTTCGTTCCATGGTAGATATCCAGAAGTATGTTGTTATATATCATGCTTTAAACTCTATTTAATGTTAATTTCTTTTCTGTTAGATTGAATTCTCGTAAATAGTTTGTGACCTACAAATAGTGTGTATATTGACTTCTTTTTTGGCATCCTCTGATTGTTCATGTGGTTCTGAAGATACCTGATTGAACATCCAGAGTACCAAACGGAAGGTGTATCCATGACACGGTTTTCATTTCAAATCCCAAAGCCTCTGCGGGAAGATTATGTGAGAAAGCGGCGCCCCAATATGAATGCTTCATGTGATGCTTCTAAGCTTGAATCTGGTGAAGGTACTTGCTTCCTATTTGTCCCTTGGCCTGCACTGTGTCTGTTAGGGGTTTCAGTTAGAGAGGAAAAGAAGGAATATTGAGATCTTGGGAGCTGGCGTGGTCTCTTTTTAGATTTAATTATCTGATGTTATTCTTTTGATTATCTTTTTTAGGCTTTATTCTTCGAAATTAGACCCCTTTGATGTATAGTTCTGGACAGGGTGATTCCTTTTATTGGTTTTTTTTCTTCTTTCTTTTGCTTTCCCTTTCATTATTCTTGCGGGTTTACTGACCAAAAGAAAGATTTTAGTTTAGCTTTTCAAAACTGTTTCTAATTTATAGATGAACAATGATGCAAGAAACTAACAATATTAAAAAAGATCAAATTCTAAGCTTTTTAATGCTCTTTCAAGATAGGTTTCAATTGGAATCTTTTTATTTTTTTCTTCTCGTTATCTGGTTCTGTTTCTTTTTTCTTAAATGAAAAGTTGGGTTTCTTGTTAAGAAAAACGAAAGATTGGTCTTTGTTCTATAAAAGTGTTATTTTCCCCTCTACATTGACCACATTTATTTGAAAGCTTTACTGATTTGTGCAGTGCGCCCAATATCATGGGCTCGTCCAGACGACTCTACTGCATTGCAGCTTGGAAAGCCTGGGCTCACCGAACTGCTTTCGACTTCACCGGTTAACAATCCCGTCACTCGACCAGCGAAGAAGGTGAAAAGAGCTCTAGCACAGGAGATGCGTAGAAGTTCTCTCACGTCGACTGAACCTCCTGCCATTAAGTTAGAACGTTGCTTTGATCAATATGAAAAATAATATTGGTGAGAACTGGTTGGTCTTGGAATTGCATTTTGTTCCTTGTTTTGTTGCTTTACATGGTTCCATCAGAGTGGGAAAGGAGATTGTAACTCATAACGATGACAAAAAGGAGGAATTTTGGAAGGGAAACTGTGACAAAGCATGTATATTATCTTAACCTTATAGGGTAATTTTTGGTCCTTGCTTGTTTGTTTTAGTTAAACAACATGTGAGGGTAAAGGATTCAAACCTCTCGAGTCTTTGACATCTTGGTCATGGGTATATGACCTTAAATCAATCTGAGTTACTACGTTCTACTTTGGATAGAGAGATTACATGAAGAGGAGCACGAGAGGAAAGAAGATGGGTCTCAAGAATGTAACTAAAGATTCTACCTCAAAAGTCTTGTATGATATGAATTTAAAAATTATTTTCAAGTACCCTAGACTGTTTTTCAGTTTTGCATATTTAAATTTAATTTCATTTTTCTAAAAAAGCTTA

mRNA sequence

ATTCAATAATTTAGAATATGACGTTGTTTTTGGACTAAAACCAATTTTCCAAATTCCATTCACGAATCAATCCACCTTCTTCTTCTTCTTCCTTCTCTTCACAACGTCTATCTGCACAATCCTTTCTCCCTCTTCTCCTTTCCCTTTTCCCGAGGCCTTACCTATCATTTCAACACCAAAATTCCCACACCCACCTTCCATTTTCCTTGGTGCTTCCGCATTTTCCATCCCCCACCTGCCTACTTCCTTGAAATCAACGCATTTCATCTCTCCTCTCTTCCTATGATTCTAAATTTGTGATTTTGGATCACAGTATGATGTGGTTAAACCGCTGATAAAATTACCATTATGGTTGGTGGGGAAGAAAATGACTTGAATGAGCCAAGTTTGTCAAACGACCAAGAAACTCCACGTCAAGATGTTATAGCTGTTTCTTCCTCTTCATCCTCTTTGTCATTTTCTTCGTCTGAGAACGAGTTACACGAGACTAGGAATGAGAATGTCCAATCTCAATCTAATGACAATCCCTCTCGTGAACTGGTTATATATGATCCGTCTGCTGATGATGGACATGCAAATGATCCTGTATCCACACCTGTAAAGCAGCAGCCTTCACCAGTTCTTGGACACTTGACTCCCAATTCTTCCAGAGTCTTACCATCGGTAGGGGCCTTTACTGTCCAATGTGCAGGCTGTTTTAAATGGAGGCTCATTCCAACGAAGGAAAAATACGAGGAAATACGAGAACATCTGTTAGAGCAACCTTTTTACTGTGAAACTGCTCGTGGGTGGCGGCCTGATATCTCCTGTGATGATCCTGCTGACATTACTCAAGATGGTAGTAGGCTCTGGGCAATTGACAAGCCGAATATAGCACAGCCCCCTCCTGGTTGGCAAAGGCTATTGCACATCAGAGGTGAAGGAAGCACCAAGTTTGCTGATGTGTATTACACTGCTCCATCAGGCAAGAAGCTTCGTTCCATGGTAGATATCCAGAAATACCTGATTGAACATCCAGAGTACCAAACGGAAGGTGTATCCATGACACGGTTTTCATTTCAAATCCCAAAGCCTCTGCGGGAAGATTATGTGAGAAAGCGGCGCCCCAATATGAATGCTTCATGTGATGCTTCTAAGCTTGAATCTGGTGAAGTGCGCCCAATATCATGGGCTCGTCCAGACGACTCTACTGCATTGCAGCTTGGAAAGCCTGGGCTCACCGAACTGCTTTCGACTTCACCGGTTAACAATCCCGTCACTCGACCAGCGAAGAAGGTGAAAAGAGCTCTAGCACAGGAGATGCGTAGAAGTTCTCTCACGTCGACTGAACCTCCTGCCATTAAGTTAGAACGTTGCTTTGATCAATATGAAAAATAATATTGGTGAGAACTGGTTGGTCTTGGAATTGCATTTTGTTCCTTGTTTTGTTGCTTTACATGGTTCCATCAGAGTGGGAAAGGAGATTGTAACTCATAACGATGACAAAAAGGAGGAATTTTGGAAGGGAAACTGTGACAAAGCATGTATATTATCTTAACCTTATAGGGTAATTTTTGGTCCTTGCTTGTTTGTTTTAGTTAAACAACATGTGAGGGTAAAGGATTCAAACCTCTCGAGTCTTTGACATCTTGGTCATGGGTATATGACCTTAAATCAATCTGAGTTACTACGTTCTACTTTGGATAGAGAGATTACATGAAGAGGAGCACGAGAGGAAAGAAGATGGGTCTCAAGAATGTAACTAAAGATTCTACCTCAAAAGTCTTGTATGATATGAATTTAAAAATTATTTTCAAGTACCCTAGACTGTTTTTCAGTTTTGCATATTTAAATTTAATTTCATTTTTCTAAAAAAGCTTA

Coding sequence (CDS)

ATGGTTGGTGGGGAAGAAAATGACTTGAATGAGCCAAGTTTGTCAAACGACCAAGAAACTCCACGTCAAGATGTTATAGCTGTTTCTTCCTCTTCATCCTCTTTGTCATTTTCTTCGTCTGAGAACGAGTTACACGAGACTAGGAATGAGAATGTCCAATCTCAATCTAATGACAATCCCTCTCGTGAACTGGTTATATATGATCCGTCTGCTGATGATGGACATGCAAATGATCCTGTATCCACACCTGTAAAGCAGCAGCCTTCACCAGTTCTTGGACACTTGACTCCCAATTCTTCCAGAGTCTTACCATCGGTAGGGGCCTTTACTGTCCAATGTGCAGGCTGTTTTAAATGGAGGCTCATTCCAACGAAGGAAAAATACGAGGAAATACGAGAACATCTGTTAGAGCAACCTTTTTACTGTGAAACTGCTCGTGGGTGGCGGCCTGATATCTCCTGTGATGATCCTGCTGACATTACTCAAGATGGTAGTAGGCTCTGGGCAATTGACAAGCCGAATATAGCACAGCCCCCTCCTGGTTGGCAAAGGCTATTGCACATCAGAGGTGAAGGAAGCACCAAGTTTGCTGATGTGTATTACACTGCTCCATCAGGCAAGAAGCTTCGTTCCATGGTAGATATCCAGAAATACCTGATTGAACATCCAGAGTACCAAACGGAAGGTGTATCCATGACACGGTTTTCATTTCAAATCCCAAAGCCTCTGCGGGAAGATTATGTGAGAAAGCGGCGCCCCAATATGAATGCTTCATGTGATGCTTCTAAGCTTGAATCTGGTGAAGTGCGCCCAATATCATGGGCTCGTCCAGACGACTCTACTGCATTGCAGCTTGGAAAGCCTGGGCTCACCGAACTGCTTTCGACTTCACCGGTTAACAATCCCGTCACTCGACCAGCGAAGAAGGTGAAAAGAGCTCTAGCACAGGAGATGCGTAGAAGTTCTCTCACGTCGACTGAACCTCCTGCCATTAAGTTAGAACGTTGCTTTGATCAATATGAAAAATAA

Protein sequence

MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLSFSSSENELHETRNENVQSQSNDNPSRELVIYDPSADDGHANDPVSTPVKQQPSPVLGHLTPNSSRVLPSVGAFTVQCAGCFKWRLIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQDGSRLWAIDKPNIAQPPPGWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQIPKPLREDYVRKRRPNMNASCDASKLESGEVRPISWARPDDSTALQLGKPGLTELLSTSPVNNPVTRPAKKVKRALAQEMRRSSLTSTEPPAIKLERCFDQYEK*

Homology

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BLAST of CSPI02G06590 vs. ExPASy Swiss-Prot
Match: Q8LA53 (Methyl-CpG-binding domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=MBD2 PE=1 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 4.2e-75
Identity = 146/269 (54.28%), Postives = 187/269 (69.52%), Query Frame = 0


Query: 47  TRNENVQSQSNDNPSR---ELVIYDPSADDGHANDPVSTPVKQQPSPVLGHLTPNSSRVL 106
           +++  VQ  S+ N  R   +LV+YD   +D    + +  PV+ QP         +S    
Sbjct: 4   SQSRAVQRSSSPNEDRGENQLVVYDLKGNDDTEEEVL--PVQSQPL--------SSRTQC 63

Query: 107 PSVGAFTVQCAGCFKWRLIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQD 166
           PS+GAFTVQCA CFKWRL+P+ +KYEEIRE LLE PF+C+TAR W+PDISCD PADI QD
Sbjct: 64  PSIGAFTVQCASCFKWRLMPSMQKYEEIREQLLENPFFCDTAREWKPDISCDVPADIYQD 123

Query: 167 GSRLWAIDKPNIAQPPPGWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHP 226
           G+RLWAIDKPNI++PP GWQRLL IRGEG T+FADVYY APSGKKLRS V++QKYL ++ 
Sbjct: 124 GTRLWAIDKPNISRPPAGWQRLLRIRGEGGTRFADVYYVAPSGKKLRSTVEVQKYLNDNS 183

Query: 227 EYQTEGVSMTRFSFQIPKPLREDYVRKRRPNMNASCDASKLE-SGEVRPISWARPDDSTA 286
           EY  EGV +++FSFQIPKPL++DYVRKR   +  S D +    + E  P++W  PDD  +
Sbjct: 184 EYIGEGVKLSQFSFQIPKPLQDDYVRKRPARLLDSIDNTNTPVAKEANPLAWISPDDHIS 243

Query: 287 LQLGKPGLTELLSTSPVNNPVTRPAKKVK 312
           LQLG P      + S +NN   +P+KK K
Sbjct: 244 LQLGTP------TESGLNNSHYQPSKKKK 256

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BLAST of CSPI02G06590 vs. ExPASy Swiss-Prot
Match: Q9FZP6 (Putative methyl-CpG-binding domain protein 12 OS=Arabidopsis thaliana OX=3702 GN=MBD12 PE=3 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 6.5e-36
Identity = 74/141 (52.48%), Postives = 95/141 (67.38%), Query Frame = 0


Query: 111 VQCAGCFKWRLIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQDGSRLWAI 170
           VQC  C KWRLIP+ + Y  I+E  L+ PF C T  GW P++SC+ P D T   +  W  
Sbjct: 2   VQCTDCKKWRLIPSMQHYNIIKETQLQTPFVCGTTSGWTPNMSCNVPQDGTTCDT--W-- 61

Query: 171 DKPNIAQPPPGWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGV 230
             P+I   P GW R +HIR E STKFADVYY  PSG++LRS  ++Q +L  HPEY  EGV
Sbjct: 62  --PSIPPIPTGWSRSVHIRSE-STKFADVYYFPPSGERLRSSAEVQSFLDNHPEYVREGV 121

Query: 231 SMTRFSFQIPKPLREDYVRKR 252
           + ++FSFQIPKPL ++YV+KR
Sbjct: 122 NRSQFSFQIPKPLDDNYVKKR 135

[Close]

BLAST of CSPI02G06590 vs. ExPASy Swiss-Prot
Match: Q9LYB9 (Methyl-CpG-binding domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=MBD4 PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.0e-25
Identity = 55/140 (39.29%), Postives = 86/140 (61.43%), Query Frame = 0


Query: 106 VGAFTVQCAGCFKWRLIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQDGS 165
           +  +  QC  C KWR+I ++E+YE+IR  +LE PF C+  +G    +SC++PADI  D S
Sbjct: 25  IDTYAAQCDNCHKWRVIDSQEEYEDIRSKMLEDPFNCQKKQG----MSCEEPADIDYDSS 84

Query: 166 RLWAIDKPNIAQPPPGWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEY 225
           R W IDKP + + P G++R L +R + S    D YY  P+GKKLRS  +I  ++  +PE+
Sbjct: 85  RTWVIDKPGLPKTPKGFKRSLVLRKDYSK--MDTYYFTPTGKKLRSRNEIAAFVEANPEF 144

Query: 226 QTEGVSMTRFSFQIPKPLRE 246
           +     +  F+F +PK + +
Sbjct: 145 R--NAPLGDFNFTVPKVMED 156

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BLAST of CSPI02G06590 vs. ExPASy Swiss-Prot
Match: Q5XEN5 (Methyl-CpG-binding domain-containing protein 1 OS=Arabidopsis thaliana OX=3702 GN=MBD1 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 5.2e-25
Identity = 55/141 (39.01%), Postives = 84/141 (59.57%), Query Frame = 0


Query: 105 SVGAFTVQCAGCFKWRLIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQDG 164
           S+  F VQC  C KWR I T+++YE+IR  + E PF+C+T  G    +SC+D  D+  D 
Sbjct: 51  SIDVFAVQCEKCMKWRKIDTQDEYEDIRSRVQEDPFFCKTKEG----VSCEDVGDLNYDS 110

Query: 165 SRLWAIDKPNIAQPPPGWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPE 224
           SR W IDKP + + P G++R L +R + S    D YY  P+GKKL+S  +I  ++  + +
Sbjct: 111 SRTWVIDKPGLPRTPRGFKRSLILRKDYSK--MDAYYITPTGKKLKSRNEIAAFIDANQD 170

Query: 225 YQTEGVSMTRFSFQIPKPLRE 246
           Y+     +  F+F +PK + E
Sbjct: 171 YKY--ALLGDFNFTVPKVMEE 183

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BLAST of CSPI02G06590 vs. ExPASy Swiss-Prot
Match: Q9UBB5 (Methyl-CpG-binding domain protein 2 OS=Homo sapiens OX=9606 GN=MBD2 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 4.6e-05
Identity = 43/152 (28.29%), Postives = 67/152 (44.08%), Query Frame = 0


Query: 161 TQDGSRLWAIDKPNIAQPPPGWQRLLHIRGEG-STKFADVYYTAPSGKKLRSMVDIQKYL 220
           T+ G R+   D P +   PPGW++   IR  G S   +DVYY +PSGKK RS   + +YL
Sbjct: 144 TESGKRM---DCPAL---PPGWKKEEVIRKSGLSAGKSDVYYFSPSGKKFRSKPQLARYL 203

Query: 221 IEHPEYQTEGVSMTRFSFQIPKPLREDYVRKRRPNMNASCDASKLESGEVRPISWARPDD 280
                     V ++ F F+  K +     + ++   N   + +K            +PD 
Sbjct: 204 -------GNTVDLSSFDFRTGKMMPSKLQKNKQRLRNDPLNQNK-----------GKPDL 263

Query: 281 STALQLGKPGLTELLSTSPVNNPVTRPAKKVK 312
           +T L + +   T  +   PV      P+ KVK
Sbjct: 264 NTTLPIRQ---TASIFKQPVTKVTNHPSNKVK 268

[Close]

BLAST of CSPI02G06590 vs. ExPASy TrEMBL
Match: A0A0A0LHE0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G062680 PE=4 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 2.1e-194
Identity = 340/342 (99.42%), Postives = 341/342 (99.71%), Query Frame = 0


Query: 1   MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLSFSSSENELHETRNENVQSQSNDNP 60
           MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLS SSSENELHETRNENVQSQSNDNP
Sbjct: 1   MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLSSSSSENELHETRNENVQSQSNDNP 60

Query: 61  SRELVIYDPSADDGHANDPVSTPVKQQPSPVLGHLTPNSSRVLPSVGAFTVQCAGCFKWR 120
           SRELVIYDPSADDGHANDPVSTPVKQQPSPVLGHLTPNSSRVLPSVGAFTVQCAGCFKWR
Sbjct: 61  SRELVIYDPSADDGHANDPVSTPVKQQPSPVLGHLTPNSSRVLPSVGAFTVQCAGCFKWR 120

Query: 121 LIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQDGSRLWAIDKPNIAQPPP 180
           LIPTKEKYEEIREHLLE+PFYCETARGWRPDISCDDPADITQDGSRLWAIDKPNIAQPPP
Sbjct: 121 LIPTKEKYEEIREHLLEKPFYCETARGWRPDISCDDPADITQDGSRLWAIDKPNIAQPPP 180

Query: 181 GWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQIP 240
           GWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQIP
Sbjct: 181 GWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQIP 240

Query: 241 KPLREDYVRKRRPNMNASCDASKLESGEVRPISWARPDDSTALQLGKPGLTELLSTSPVN 300
           KPLREDYVRKRRPNMNASCDASKLESGEVRPISWARPDDSTALQLGKPGLTELLSTSPVN
Sbjct: 241 KPLREDYVRKRRPNMNASCDASKLESGEVRPISWARPDDSTALQLGKPGLTELLSTSPVN 300

Query: 301 NPVTRPAKKVKRALAQEMRRSSLTSTEPPAIKLERCFDQYEK 343
           NPVTRPAKKVKRALAQEMRRSSLTSTEPPAIKLERCFDQYEK
Sbjct: 301 NPVTRPAKKVKRALAQEMRRSSLTSTEPPAIKLERCFDQYEK 342

[Close]

BLAST of CSPI02G06590 vs. ExPASy TrEMBL
Match: A0A5D3CST2 (Methyl-CpG-binding domain-containing protein 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2754G00130 PE=4 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 8.2e-183
Identity = 323/342 (94.44%), Postives = 332/342 (97.08%), Query Frame = 0


Query: 1   MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLSFSSSENELHETRNENVQSQSNDNP 60
           MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLS SSSENELHETRNENVQSQ N+N 
Sbjct: 1   MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLS-SSSENELHETRNENVQSQPNENS 60

Query: 61  SRELVIYDPSADDGHANDPVSTPVKQQPSPVLGHLTPNSSRVLPSVGAFTVQCAGCFKWR 120
           SRELVIY+PSA DG ANDPV+TPVKQQPSP+LGHLTPNS+RVLPSVGAFTVQCAGCFKWR
Sbjct: 61  SRELVIYNPSAKDGRANDPVATPVKQQPSPLLGHLTPNSTRVLPSVGAFTVQCAGCFKWR 120

Query: 121 LIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQDGSRLWAIDKPNIAQPPP 180
           LIPTKEKYEEIREHLLEQPFYCETAR WRPDI+CDDPADITQDGSRLWAIDKPNIAQPPP
Sbjct: 121 LIPTKEKYEEIREHLLEQPFYCETAREWRPDITCDDPADITQDGSRLWAIDKPNIAQPPP 180

Query: 181 GWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQIP 240
           GWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQIP
Sbjct: 181 GWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQIP 240

Query: 241 KPLREDYVRKRRPNMNASCDASKLESGEVRPISWARPDDSTALQLGKPGLTELLSTSPVN 300
           KPLREDYVRKRRPN+NASCDASKLESGEVRPISWARPDDSTALQLGKPGL ELLSTSPV+
Sbjct: 241 KPLREDYVRKRRPNINASCDASKLESGEVRPISWARPDDSTALQLGKPGLPELLSTSPVD 300

Query: 301 NPVTRPAKKVKRALAQEMRRSSLTSTEPPAIKLERCFDQYEK 343
           +PVTRPAKKVKRAL QEMRRSSL STEPPAIKLE CFDQYEK
Sbjct: 301 HPVTRPAKKVKRALGQEMRRSSLASTEPPAIKLEGCFDQYEK 341

[Close]

BLAST of CSPI02G06590 vs. ExPASy TrEMBL
Match: A0A1S3BR32 (methyl-CpG-binding domain-containing protein 2-like OS=Cucumis melo OX=3656 GN=LOC103492271 PE=4 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 8.2e-183
Identity = 323/342 (94.44%), Postives = 332/342 (97.08%), Query Frame = 0


Query: 1   MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLSFSSSENELHETRNENVQSQSNDNP 60
           MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLS SSSENELHETRNENVQSQ N+N 
Sbjct: 1   MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLS-SSSENELHETRNENVQSQPNENS 60

Query: 61  SRELVIYDPSADDGHANDPVSTPVKQQPSPVLGHLTPNSSRVLPSVGAFTVQCAGCFKWR 120
           SRELVIY+PSA DG ANDPV+TPVKQQPSP+LGHLTPNS+RVLPSVGAFTVQCAGCFKWR
Sbjct: 61  SRELVIYNPSAKDGRANDPVATPVKQQPSPLLGHLTPNSTRVLPSVGAFTVQCAGCFKWR 120

Query: 121 LIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQDGSRLWAIDKPNIAQPPP 180
           LIPTKEKYEEIREHLLEQPFYCETAR WRPDI+CDDPADITQDGSRLWAIDKPNIAQPPP
Sbjct: 121 LIPTKEKYEEIREHLLEQPFYCETAREWRPDITCDDPADITQDGSRLWAIDKPNIAQPPP 180

Query: 181 GWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQIP 240
           GWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQIP
Sbjct: 181 GWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQIP 240

Query: 241 KPLREDYVRKRRPNMNASCDASKLESGEVRPISWARPDDSTALQLGKPGLTELLSTSPVN 300
           KPLREDYVRKRRPN+NASCDASKLESGEVRPISWARPDDSTALQLGKPGL ELLSTSPV+
Sbjct: 241 KPLREDYVRKRRPNINASCDASKLESGEVRPISWARPDDSTALQLGKPGLPELLSTSPVD 300

Query: 301 NPVTRPAKKVKRALAQEMRRSSLTSTEPPAIKLERCFDQYEK 343
           +PVTRPAKKVKRAL QEMRRSSL STEPPAIKLE CFDQYEK
Sbjct: 301 HPVTRPAKKVKRALGQEMRRSSLASTEPPAIKLEGCFDQYEK 341

[Close]

BLAST of CSPI02G06590 vs. ExPASy TrEMBL
Match: A0A6J1EN70 (methyl-CpG-binding domain-containing protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111436133 PE=4 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 4.5e-157
Identity = 284/343 (82.80%), Postives = 312/343 (90.96%), Query Frame = 0


Query: 1   MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLSFSSSENELHETRNENVQSQSNDNP 60
           MVGGEENDLNEPSLSN QET +QDVIAVSSSSSS S SSSE+ELHETRNENVQSQSN+N 
Sbjct: 1   MVGGEENDLNEPSLSNHQETTQQDVIAVSSSSSSFSSSSSESELHETRNENVQSQSNENV 60

Query: 61  SRELVIYDPSA-DDGHANDPVSTPVKQQPSPVLGHLTPNSSRVLPSVGAFTVQCAGCFKW 120
           SRELV+Y+PSA D+G A++PVSTPV+ Q  P+LGHLTPNSSRVL  VGAFTVQCA CFKW
Sbjct: 61  SRELVLYNPSANDEGGAHEPVSTPVRHQRPPLLGHLTPNSSRVL-RVGAFTVQCASCFKW 120

Query: 121 RLIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQDGSRLWAIDKPNIAQPP 180
           RLIPTKEKYEEIREHLLE+PFYCETAR WRPDISC+DPADITQD  RLWAIDKPNIAQPP
Sbjct: 121 RLIPTKEKYEEIREHLLEEPFYCETAREWRPDISCNDPADITQDDGRLWAIDKPNIAQPP 180

Query: 181 PGWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQI 240
           PGWQRLLHIRGEGSTKFADVYY APSGK+LRSMVDIQKYLIEHPEYQTEGV MTRFSFQ 
Sbjct: 181 PGWQRLLHIRGEGSTKFADVYYIAPSGKRLRSMVDIQKYLIEHPEYQTEGVCMTRFSFQT 240

Query: 241 PKPLREDYVRKRRPNMNASCDASKLESGEVRPISWARPDDSTALQLGKPGLTELLSTSPV 300
           PKPL+E+YVRKRR NMNASCD SKLESGEV PISWARPD+STALQLG+PGL+EL  T+PV
Sbjct: 241 PKPLQENYVRKRRANMNASCDGSKLESGEVYPISWARPDNSTALQLGRPGLSELFPTAPV 300

Query: 301 NNPVTRPAKKVKRALAQEMRRSSLTSTEPPAIKLERCFDQYEK 343
           +N V++PAKK KRA+A+EM RSSL STEPP + LER F++Y+K
Sbjct: 301 DNRVSQPAKKAKRAVAKEM-RSSLASTEPPDLSLERYFNEYKK 341

[Close]

BLAST of CSPI02G06590 vs. ExPASy TrEMBL
Match: A0A6J1K3E0 (methyl-CpG-binding domain-containing protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111492010 PE=4 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.5e-155
Identity = 281/343 (81.92%), Postives = 309/343 (90.09%), Query Frame = 0


Query: 1   MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLSFSSSENELHETRNENVQSQSNDNP 60
           MVGGEENDLNEPSLSN QET +QDVIAVSSSSSS S SSSE+ELHETRNENVQSQSN+N 
Sbjct: 1   MVGGEENDLNEPSLSNHQETTQQDVIAVSSSSSSFSSSSSESELHETRNENVQSQSNENV 60

Query: 61  SRELVIYDPSA-DDGHANDPVSTPVKQQPSPVLGHLTPNSSRVLPSVGAFTVQCAGCFKW 120
           SRELV+Y+PSA D+G A++PVSTPV+ Q  P+LGHLTPNSSRVL  VGAFTVQCA CFKW
Sbjct: 61  SRELVLYNPSANDEGGAHEPVSTPVRHQRPPLLGHLTPNSSRVL-RVGAFTVQCASCFKW 120

Query: 121 RLIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQDGSRLWAIDKPNIAQPP 180
           RLIPTKEKYEEIREHLLE+PFYCETAR WRPDISC+DPADITQD  RLWAIDKPNIAQPP
Sbjct: 121 RLIPTKEKYEEIREHLLEEPFYCETAREWRPDISCNDPADITQDDGRLWAIDKPNIAQPP 180

Query: 181 PGWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQI 240
           PGWQRLLHIRGEGSTKFADVYY APSGK+LRSMVDIQKYLIEHPEYQTEGV MTRFSFQ 
Sbjct: 181 PGWQRLLHIRGEGSTKFADVYYIAPSGKRLRSMVDIQKYLIEHPEYQTEGVCMTRFSFQT 240

Query: 241 PKPLREDYVRKRRPNMNASCDASKLESGEVRPISWARPDDSTALQLGKPGLTELLSTSPV 300
           PKPL+E+YVRKRR NMNASCD SKLESGEV PISWARPD+ST LQLG+PGL+EL  T+PV
Sbjct: 241 PKPLQENYVRKRRANMNASCDGSKLESGEVYPISWARPDNSTGLQLGRPGLSELFPTAPV 300

Query: 301 NNPVTRPAKKVKRALAQEMRRSSLTSTEPPAIKLERCFDQYEK 343
           +N V++PAKK KRA+ +EM  SSL STEPP + LER F++Y+K
Sbjct: 301 DNRVSQPAKKAKRAVTKEM-HSSLASTEPPDLSLERYFNEYKK 341

[Close]

BLAST of CSPI02G06590 vs. NCBI nr
Match: XP_004150390.1 (methyl-CpG-binding domain-containing protein 2 [Cucumis sativus] >XP_031737021.1 methyl-CpG-binding domain-containing protein 2 [Cucumis sativus] >KGN61193.1 hypothetical protein Csa_006529 [Cucumis sativus])

HSP 1 Score: 688.0 bits (1774), Expect = 4.3e-194
Identity = 340/342 (99.42%), Postives = 341/342 (99.71%), Query Frame = 0


Query: 1   MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLSFSSSENELHETRNENVQSQSNDNP 60
           MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLS SSSENELHETRNENVQSQSNDNP
Sbjct: 1   MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLSSSSSENELHETRNENVQSQSNDNP 60

Query: 61  SRELVIYDPSADDGHANDPVSTPVKQQPSPVLGHLTPNSSRVLPSVGAFTVQCAGCFKWR 120
           SRELVIYDPSADDGHANDPVSTPVKQQPSPVLGHLTPNSSRVLPSVGAFTVQCAGCFKWR
Sbjct: 61  SRELVIYDPSADDGHANDPVSTPVKQQPSPVLGHLTPNSSRVLPSVGAFTVQCAGCFKWR 120

Query: 121 LIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQDGSRLWAIDKPNIAQPPP 180
           LIPTKEKYEEIREHLLE+PFYCETARGWRPDISCDDPADITQDGSRLWAIDKPNIAQPPP
Sbjct: 121 LIPTKEKYEEIREHLLEKPFYCETARGWRPDISCDDPADITQDGSRLWAIDKPNIAQPPP 180

Query: 181 GWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQIP 240
           GWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQIP
Sbjct: 181 GWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQIP 240

Query: 241 KPLREDYVRKRRPNMNASCDASKLESGEVRPISWARPDDSTALQLGKPGLTELLSTSPVN 300
           KPLREDYVRKRRPNMNASCDASKLESGEVRPISWARPDDSTALQLGKPGLTELLSTSPVN
Sbjct: 241 KPLREDYVRKRRPNMNASCDASKLESGEVRPISWARPDDSTALQLGKPGLTELLSTSPVN 300

Query: 301 NPVTRPAKKVKRALAQEMRRSSLTSTEPPAIKLERCFDQYEK 343
           NPVTRPAKKVKRALAQEMRRSSLTSTEPPAIKLERCFDQYEK
Sbjct: 301 NPVTRPAKKVKRALAQEMRRSSLTSTEPPAIKLERCFDQYEK 342

[Close]

BLAST of CSPI02G06590 vs. NCBI nr
Match: XP_008450795.1 (PREDICTED: methyl-CpG-binding domain-containing protein 2-like [Cucumis melo] >KAA0047569.1 methyl-CpG-binding domain-containing protein 2-like [Cucumis melo var. makuwa] >TYK14963.1 methyl-CpG-binding domain-containing protein 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 649.4 bits (1674), Expect = 1.7e-182
Identity = 323/342 (94.44%), Postives = 332/342 (97.08%), Query Frame = 0


Query: 1   MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLSFSSSENELHETRNENVQSQSNDNP 60
           MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLS SSSENELHETRNENVQSQ N+N 
Sbjct: 1   MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLS-SSSENELHETRNENVQSQPNENS 60

Query: 61  SRELVIYDPSADDGHANDPVSTPVKQQPSPVLGHLTPNSSRVLPSVGAFTVQCAGCFKWR 120
           SRELVIY+PSA DG ANDPV+TPVKQQPSP+LGHLTPNS+RVLPSVGAFTVQCAGCFKWR
Sbjct: 61  SRELVIYNPSAKDGRANDPVATPVKQQPSPLLGHLTPNSTRVLPSVGAFTVQCAGCFKWR 120

Query: 121 LIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQDGSRLWAIDKPNIAQPPP 180
           LIPTKEKYEEIREHLLEQPFYCETAR WRPDI+CDDPADITQDGSRLWAIDKPNIAQPPP
Sbjct: 121 LIPTKEKYEEIREHLLEQPFYCETAREWRPDITCDDPADITQDGSRLWAIDKPNIAQPPP 180

Query: 181 GWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQIP 240
           GWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQIP
Sbjct: 181 GWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQIP 240

Query: 241 KPLREDYVRKRRPNMNASCDASKLESGEVRPISWARPDDSTALQLGKPGLTELLSTSPVN 300
           KPLREDYVRKRRPN+NASCDASKLESGEVRPISWARPDDSTALQLGKPGL ELLSTSPV+
Sbjct: 241 KPLREDYVRKRRPNINASCDASKLESGEVRPISWARPDDSTALQLGKPGLPELLSTSPVD 300

Query: 301 NPVTRPAKKVKRALAQEMRRSSLTSTEPPAIKLERCFDQYEK 343
           +PVTRPAKKVKRAL QEMRRSSL STEPPAIKLE CFDQYEK
Sbjct: 301 HPVTRPAKKVKRALGQEMRRSSLASTEPPAIKLEGCFDQYEK 341

[Close]

BLAST of CSPI02G06590 vs. NCBI nr
Match: XP_038890273.1 (methyl-CpG-binding domain-containing protein 2 isoform X1 [Benincasa hispida] >XP_038890274.1 methyl-CpG-binding domain-containing protein 2 isoform X1 [Benincasa hispida] >XP_038890275.1 methyl-CpG-binding domain-containing protein 2 isoform X1 [Benincasa hispida] >XP_038890276.1 methyl-CpG-binding domain-containing protein 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 611.7 bits (1576), Expect = 3.9e-171
Identity = 306/356 (85.96%), Postives = 321/356 (90.17%), Query Frame = 0


Query: 1   MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLSFSSSENELHETRNE---------- 60
           MVGGEENDLNEPSLSN QETPRQDV AVSS SSSLS SS ENELHE RNE          
Sbjct: 1   MVGGEENDLNEPSLSNGQETPRQDVRAVSSFSSSLSSSSFENELHEPRNENAQSQSNENP 60

Query: 61  ----NVQSQSNDNPSRELVIYDPSADDGHANDPVSTPVKQQPSPVLGHLTPNSSRVLPSV 120
               N QSQSN+NPSRELV+YD SA+DG AN+PVSTPV+ Q  P+LGHLTPNSSRVLPSV
Sbjct: 61  SRNKNAQSQSNENPSRELVLYDSSANDGGANEPVSTPVEHQRPPLLGHLTPNSSRVLPSV 120

Query: 121 GAFTVQCAGCFKWRLIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQDGSR 180
           GAFTVQCA CFKWRLIPTKEKYEEIREHLLE+PFYCETAR WRPDISCDDPADITQDGSR
Sbjct: 121 GAFTVQCASCFKWRLIPTKEKYEEIREHLLEKPFYCETAREWRPDISCDDPADITQDGSR 180

Query: 181 LWAIDKPNIAQPPPGWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQ 240
           LWAIDKPNIAQPPPGWQRLLHIRGEGSTKFADVYY APSGKKLRSMVDIQKYLIEHPEYQ
Sbjct: 181 LWAIDKPNIAQPPPGWQRLLHIRGEGSTKFADVYYIAPSGKKLRSMVDIQKYLIEHPEYQ 240

Query: 241 TEGVSMTRFSFQIPKPLREDYVRKRRPNMNASCDASKLESGEVRPISWARPDDSTALQLG 300
           TEGVSMTRFSFQIPKPLRE+YVRKRRPNMNASCD SKLESG V PISWARPDDSTALQLG
Sbjct: 241 TEGVSMTRFSFQIPKPLRENYVRKRRPNMNASCDGSKLESGGVHPISWARPDDSTALQLG 300

Query: 301 KPGLTELLSTSPVNNPVTRPAKKVKRALAQEMRRSSLTSTEPPAIKLERCFDQYEK 343
           KPGL+ELL T+PV+NP+TRP KKVKRALA+EMRRSSL STEPPA+KLERCFD+YEK
Sbjct: 301 KPGLSELLLTAPVDNPITRPVKKVKRALAREMRRSSLASTEPPAVKLERCFDKYEK 356

[Close]

BLAST of CSPI02G06590 vs. NCBI nr
Match: XP_022929597.1 (methyl-CpG-binding domain-containing protein 2-like [Cucurbita moschata] >XP_022929598.1 methyl-CpG-binding domain-containing protein 2-like [Cucurbita moschata])

HSP 1 Score: 563.9 bits (1452), Expect = 9.3e-157
Identity = 284/343 (82.80%), Postives = 312/343 (90.96%), Query Frame = 0


Query: 1   MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLSFSSSENELHETRNENVQSQSNDNP 60
           MVGGEENDLNEPSLSN QET +QDVIAVSSSSSS S SSSE+ELHETRNENVQSQSN+N 
Sbjct: 1   MVGGEENDLNEPSLSNHQETTQQDVIAVSSSSSSFSSSSSESELHETRNENVQSQSNENV 60

Query: 61  SRELVIYDPSA-DDGHANDPVSTPVKQQPSPVLGHLTPNSSRVLPSVGAFTVQCAGCFKW 120
           SRELV+Y+PSA D+G A++PVSTPV+ Q  P+LGHLTPNSSRVL  VGAFTVQCA CFKW
Sbjct: 61  SRELVLYNPSANDEGGAHEPVSTPVRHQRPPLLGHLTPNSSRVL-RVGAFTVQCASCFKW 120

Query: 121 RLIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQDGSRLWAIDKPNIAQPP 180
           RLIPTKEKYEEIREHLLE+PFYCETAR WRPDISC+DPADITQD  RLWAIDKPNIAQPP
Sbjct: 121 RLIPTKEKYEEIREHLLEEPFYCETAREWRPDISCNDPADITQDDGRLWAIDKPNIAQPP 180

Query: 181 PGWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQI 240
           PGWQRLLHIRGEGSTKFADVYY APSGK+LRSMVDIQKYLIEHPEYQTEGV MTRFSFQ 
Sbjct: 181 PGWQRLLHIRGEGSTKFADVYYIAPSGKRLRSMVDIQKYLIEHPEYQTEGVCMTRFSFQT 240

Query: 241 PKPLREDYVRKRRPNMNASCDASKLESGEVRPISWARPDDSTALQLGKPGLTELLSTSPV 300
           PKPL+E+YVRKRR NMNASCD SKLESGEV PISWARPD+STALQLG+PGL+EL  T+PV
Sbjct: 241 PKPLQENYVRKRRANMNASCDGSKLESGEVYPISWARPDNSTALQLGRPGLSELFPTAPV 300

Query: 301 NNPVTRPAKKVKRALAQEMRRSSLTSTEPPAIKLERCFDQYEK 343
           +N V++PAKK KRA+A+EM RSSL STEPP + LER F++Y+K
Sbjct: 301 DNRVSQPAKKAKRAVAKEM-RSSLASTEPPDLSLERYFNEYKK 341

[Close]

BLAST of CSPI02G06590 vs. NCBI nr
Match: XP_023546682.1 (methyl-CpG-binding domain-containing protein 2-like [Cucurbita pepo subsp. pepo] >XP_023546683.1 methyl-CpG-binding domain-containing protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 562.4 bits (1448), Expect = 2.7e-156
Identity = 283/343 (82.51%), Postives = 311/343 (90.67%), Query Frame = 0


Query: 1   MVGGEENDLNEPSLSNDQETPRQDVIAVSSSSSSLSFSSSENELHETRNENVQSQSNDNP 60
           MVGGEENDLNEPSLSN QET +QDVIAVSSSSSS S SSSE+ELHETRNENVQSQSN+N 
Sbjct: 1   MVGGEENDLNEPSLSNHQETTQQDVIAVSSSSSSFSSSSSESELHETRNENVQSQSNENV 60

Query: 61  SRELVIYDPSA-DDGHANDPVSTPVKQQPSPVLGHLTPNSSRVLPSVGAFTVQCAGCFKW 120
           SRELV+Y+PSA D+G A++PVSTPV+ Q  P+LGHLTPNSSRVL  VGAFTVQCA CFKW
Sbjct: 61  SRELVLYNPSANDEGGAHEPVSTPVRHQRPPLLGHLTPNSSRVL-RVGAFTVQCASCFKW 120

Query: 121 RLIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQDGSRLWAIDKPNIAQPP 180
           RLIPTKEKYEEIREHLLE+PFYCETAR WRPDISC+DPADITQD  RLWAIDKPNIAQPP
Sbjct: 121 RLIPTKEKYEEIREHLLEEPFYCETAREWRPDISCNDPADITQDDGRLWAIDKPNIAQPP 180

Query: 181 PGWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGVSMTRFSFQI 240
           PGWQRLLHIRGEGSTKFADVYY APSGK+LRSMVDIQKYLIEHPEYQTEGV MTRFSFQ 
Sbjct: 181 PGWQRLLHIRGEGSTKFADVYYIAPSGKRLRSMVDIQKYLIEHPEYQTEGVCMTRFSFQT 240

Query: 241 PKPLREDYVRKRRPNMNASCDASKLESGEVRPISWARPDDSTALQLGKPGLTELLSTSPV 300
           PKPL+E+YVRKRR NMNASCD SKLESGEV PISWARPD+STALQLG+PGL+EL  T+PV
Sbjct: 241 PKPLQENYVRKRRANMNASCDGSKLESGEVYPISWARPDNSTALQLGRPGLSELFPTAPV 300

Query: 301 NNPVTRPAKKVKRALAQEMRRSSLTSTEPPAIKLERCFDQYEK 343
           +N  ++PAKK KRA+A+EM RSSL STEPP + LER F++Y+K
Sbjct: 301 DNRASQPAKKAKRAVAKEM-RSSLASTEPPDLSLERYFNEYKK 341

[Close]

BLAST of CSPI02G06590 vs. TAIR 10
Match: AT5G35330.1 (methyl-CPG-binding domain protein 02 )

HSP 1 Score: 283.1 bits (723), Expect = 3.0e-76
Identity = 146/269 (54.28%), Postives = 187/269 (69.52%), Query Frame = 0


Query: 47  TRNENVQSQSNDNPSR---ELVIYDPSADDGHANDPVSTPVKQQPSPVLGHLTPNSSRVL 106
           +++  VQ  S+ N  R   +LV+YD   +D    + +  PV+ QP         +S    
Sbjct: 4   SQSRAVQRSSSPNEDRGENQLVVYDLKGNDDTEEEVL--PVQSQPL--------SSRTQC 63

Query: 107 PSVGAFTVQCAGCFKWRLIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQD 166
           PS+GAFTVQCA CFKWRL+P+ +KYEEIRE LLE PF+C+TAR W+PDISCD PADI QD
Sbjct: 64  PSIGAFTVQCASCFKWRLMPSMQKYEEIREQLLENPFFCDTAREWKPDISCDVPADIYQD 123

Query: 167 GSRLWAIDKPNIAQPPPGWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHP 226
           G+RLWAIDKPNI++PP GWQRLL IRGEG T+FADVYY APSGKKLRS V++QKYL ++ 
Sbjct: 124 GTRLWAIDKPNISRPPAGWQRLLRIRGEGGTRFADVYYVAPSGKKLRSTVEVQKYLNDNS 183

Query: 227 EYQTEGVSMTRFSFQIPKPLREDYVRKRRPNMNASCDASKLE-SGEVRPISWARPDDSTA 286
           EY  EGV +++FSFQIPKPL++DYVRKR   +  S D +    + E  P++W  PDD  +
Sbjct: 184 EYIGEGVKLSQFSFQIPKPLQDDYVRKRPARLLDSIDNTNTPVAKEANPLAWISPDDHIS 243

Query: 287 LQLGKPGLTELLSTSPVNNPVTRPAKKVK 312
           LQLG P      + S +NN   +P+KK K
Sbjct: 244 LQLGTP------TESGLNNSHYQPSKKKK 256

[Close]

BLAST of CSPI02G06590 vs. TAIR 10
Match: AT5G35330.2 (methyl-CPG-binding domain protein 02 )

HSP 1 Score: 283.1 bits (723), Expect = 3.0e-76
Identity = 146/269 (54.28%), Postives = 187/269 (69.52%), Query Frame = 0


Query: 47  TRNENVQSQSNDNPSR---ELVIYDPSADDGHANDPVSTPVKQQPSPVLGHLTPNSSRVL 106
           +++  VQ  S+ N  R   +LV+YD   +D    + +  PV+ QP         +S    
Sbjct: 4   SQSRAVQRSSSPNEDRGENQLVVYDLKGNDDTEEEVL--PVQSQPL--------SSRTQC 63

Query: 107 PSVGAFTVQCAGCFKWRLIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQD 166
           PS+GAFTVQCA CFKWRL+P+ +KYEEIRE LLE PF+C+TAR W+PDISCD PADI QD
Sbjct: 64  PSIGAFTVQCASCFKWRLMPSMQKYEEIREQLLENPFFCDTAREWKPDISCDVPADIYQD 123

Query: 167 GSRLWAIDKPNIAQPPPGWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHP 226
           G+RLWAIDKPNI++PP GWQRLL IRGEG T+FADVYY APSGKKLRS V++QKYL ++ 
Sbjct: 124 GTRLWAIDKPNISRPPAGWQRLLRIRGEGGTRFADVYYVAPSGKKLRSTVEVQKYLNDNS 183

Query: 227 EYQTEGVSMTRFSFQIPKPLREDYVRKRRPNMNASCDASKLE-SGEVRPISWARPDDSTA 286
           EY  EGV +++FSFQIPKPL++DYVRKR   +  S D +    + E  P++W  PDD  +
Sbjct: 184 EYIGEGVKLSQFSFQIPKPLQDDYVRKRPARLLDSIDNTNTPVAKEANPLAWISPDDHIS 243

Query: 287 LQLGKPGLTELLSTSPVNNPVTRPAKKVK 312
           LQLG P      + S +NN   +P+KK K
Sbjct: 244 LQLGTP------TESGLNNSHYQPSKKKK 256

[Close]

BLAST of CSPI02G06590 vs. TAIR 10
Match: AT5G35330.3 (methyl-CPG-binding domain protein 02 )

HSP 1 Score: 283.1 bits (723), Expect = 3.0e-76
Identity = 146/269 (54.28%), Postives = 187/269 (69.52%), Query Frame = 0


Query: 47  TRNENVQSQSNDNPSR---ELVIYDPSADDGHANDPVSTPVKQQPSPVLGHLTPNSSRVL 106
           +++  VQ  S+ N  R   +LV+YD   +D    + +  PV+ QP         +S    
Sbjct: 4   SQSRAVQRSSSPNEDRGENQLVVYDLKGNDDTEEEVL--PVQSQPL--------SSRTQC 63

Query: 107 PSVGAFTVQCAGCFKWRLIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQD 166
           PS+GAFTVQCA CFKWRL+P+ +KYEEIRE LLE PF+C+TAR W+PDISCD PADI QD
Sbjct: 64  PSIGAFTVQCASCFKWRLMPSMQKYEEIREQLLENPFFCDTAREWKPDISCDVPADIYQD 123

Query: 167 GSRLWAIDKPNIAQPPPGWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHP 226
           G+RLWAIDKPNI++PP GWQRLL IRGEG T+FADVYY APSGKKLRS V++QKYL ++ 
Sbjct: 124 GTRLWAIDKPNISRPPAGWQRLLRIRGEGGTRFADVYYVAPSGKKLRSTVEVQKYLNDNS 183

Query: 227 EYQTEGVSMTRFSFQIPKPLREDYVRKRRPNMNASCDASKLE-SGEVRPISWARPDDSTA 286
           EY  EGV +++FSFQIPKPL++DYVRKR   +  S D +    + E  P++W  PDD  +
Sbjct: 184 EYIGEGVKLSQFSFQIPKPLQDDYVRKRPARLLDSIDNTNTPVAKEANPLAWISPDDHIS 243

Query: 287 LQLGKPGLTELLSTSPVNNPVTRPAKKVK 312
           LQLG P      + S +NN   +P+KK K
Sbjct: 244 LQLGTP------TESGLNNSHYQPSKKKK 256

[Close]

BLAST of CSPI02G06590 vs. TAIR 10
Match: AT5G35338.2 (methyl-CPG-binding domain 12 )

HSP 1 Score: 152.9 bits (385), Expect = 4.6e-37
Identity = 74/141 (52.48%), Postives = 95/141 (67.38%), Query Frame = 0


Query: 111 VQCAGCFKWRLIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQDGSRLWAI 170
           VQC  C KWRLIP+ + Y  I+E  L+ PF C T  GW P++SC+ P D T   +  W  
Sbjct: 2   VQCTDCKKWRLIPSMQHYNIIKETQLQTPFVCGTTSGWTPNMSCNVPQDGTTCDT--W-- 61

Query: 171 DKPNIAQPPPGWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEYQTEGV 230
             P+I   P GW R +HIR E STKFADVYY  PSG++LRS  ++Q +L  HPEY  EGV
Sbjct: 62  --PSIPPIPTGWSRSVHIRSE-STKFADVYYFPPSGERLRSSAEVQSFLDNHPEYVREGV 121

Query: 231 SMTRFSFQIPKPLREDYVRKR 252
           + ++FSFQIPKPL ++YV+KR
Sbjct: 122 NRSQFSFQIPKPLDDNYVKKR 135

[Close]

BLAST of CSPI02G06590 vs. TAIR 10
Match: AT3G63030.1 (methyl-CPG-binding domain 4 )

HSP 1 Score: 119.0 bits (297), Expect = 7.4e-27
Identity = 55/140 (39.29%), Postives = 86/140 (61.43%), Query Frame = 0


Query: 106 VGAFTVQCAGCFKWRLIPTKEKYEEIREHLLEQPFYCETARGWRPDISCDDPADITQDGS 165
           +  +  QC  C KWR+I ++E+YE+IR  +LE PF C+  +G    +SC++PADI  D S
Sbjct: 25  IDTYAAQCDNCHKWRVIDSQEEYEDIRSKMLEDPFNCQKKQG----MSCEEPADIDYDSS 84

Query: 166 RLWAIDKPNIAQPPPGWQRLLHIRGEGSTKFADVYYTAPSGKKLRSMVDIQKYLIEHPEY 225
           R W IDKP + + P G++R L +R + S    D YY  P+GKKLRS  +I  ++  +PE+
Sbjct: 85  RTWVIDKPGLPKTPKGFKRSLVLRKDYSK--MDTYYFTPTGKKLRSRNEIAAFVEANPEF 144

Query: 226 QTEGVSMTRFSFQIPKPLRE 246
           +     +  F+F +PK + +
Sbjct: 145 R--NAPLGDFNFTVPKVMED 156

The following BLAST results are available for this feature:

BLAST of CSPI02G06590 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LA53	4.2e-75	54.28	Methyl-CpG-binding domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q9FZP6	6.5e-36	52.48	Putative methyl-CpG-binding domain protein 12 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q9LYB9	1.0e-25	39.29	Methyl-CpG-binding domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q5XEN5	5.2e-25	39.01	Methyl-CpG-binding domain-containing protein 1 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q9UBB5	4.6e-05	28.29	Methyl-CpG-binding domain protein 2 OS=Homo sapiens OX=9606 GN=MBD2 PE=1 SV=1	[more]

BLAST of CSPI02G06590 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHE0	2.1e-194	99.42	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G062680 PE=4 SV=1	[more]
A0A5D3CST2	8.2e-183	94.44	Methyl-CpG-binding domain-containing protein 2-like OS=Cucumis melo var. makuwa ...	[more]
A0A1S3BR32	8.2e-183	94.44	methyl-CpG-binding domain-containing protein 2-like OS=Cucumis melo OX=3656 GN=L...	[more]
A0A6J1EN70	4.5e-157	82.80	methyl-CpG-binding domain-containing protein 2-like OS=Cucurbita moschata OX=366...	[more]
A0A6J1K3E0	1.5e-155	81.92	methyl-CpG-binding domain-containing protein 2-like OS=Cucurbita maxima OX=3661 ...	[more]

BLAST of CSPI02G06590 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004150390.1	4.3e-194	99.42	methyl-CpG-binding domain-containing protein 2 [Cucumis sativus] >XP_031737021.1...	[more]
XP_008450795.1	1.7e-182	94.44	PREDICTED: methyl-CpG-binding domain-containing protein 2-like [Cucumis melo] >K...	[more]
XP_038890273.1	3.9e-171	85.96	methyl-CpG-binding domain-containing protein 2 isoform X1 [Benincasa hispida] >X...	[more]
XP_022929597.1	9.3e-157	82.80	methyl-CpG-binding domain-containing protein 2-like [Cucurbita moschata] >XP_022...	[more]
XP_023546682.1	2.7e-156	82.51	methyl-CpG-binding domain-containing protein 2-like [Cucurbita pepo subsp. pepo]...	[more]

BLAST of CSPI02G06590 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35330.1	3.0e-76	54.28	methyl-CPG-binding domain protein 02	[more]
AT5G35330.2	3.0e-76	54.28	methyl-CPG-binding domain protein 02	[more]
AT5G35330.3	3.0e-76	54.28	methyl-CPG-binding domain protein 02	[more]
AT5G35338.2	4.6e-37	52.48	methyl-CPG-binding domain 12	[more]
AT3G63030.1	7.4e-27	39.29	methyl-CPG-binding domain 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001739	Methyl-CpG DNA binding	SMART	SM00391	TAM_2	coord: 172..247 e-value: 4.7E-5 score: 32.4
IPR001739	Methyl-CpG DNA binding	PFAM	PF01429	MBD	coord: 179..238 e-value: 2.4E-10 score: 40.0
IPR001739	Methyl-CpG DNA binding	PROSITE	PS50982	MBD	coord: 168..242 score: 16.711006
None	No IPR available	GENE3D	3.30.40.100		coord: 107..161 e-value: 1.8E-5 score: 26.2
None	No IPR available	GENE3D	3.30.890.10		coord: 162..270 e-value: 1.3E-19 score: 72.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 9..42
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 80..96
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..96
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 49..63
None	No IPR available	PANTHER	PTHR12396:SF41	ZINC FINGER, CW-TYPE, DNA-BINDING DOMAIN PROTEIN-RELATED	coord: 31..288
None	No IPR available	PANTHER	PTHR12396	METHYL-CPG BINDING PROTEIN, MBD	coord: 31..288
None	No IPR available	CDD	cd01396	MeCP2_MBD	coord: 172..251 e-value: 2.31388E-26 score: 98.2136
IPR011124	Zinc finger, CW-type	PFAM	PF07496	zf-CW	coord: 109..159 e-value: 1.4E-12 score: 47.4
IPR011124	Zinc finger, CW-type	PROSITE	PS51050	ZF_CW	coord: 103..162 score: 12.551763
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 148..254

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G06590.1	CSPI02G06590.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0008270	zinc ion binding