CSPI02G05270 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G05270
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionTranslation initiation factor IF-3
LocationChr2: 3739969 .. 3743100 (-)
RNA-Seq ExpressionCSPI02G05270
SyntenyCSPI02G05270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGGAATATAGAGTTAGAGTGATGAGTCGACTCCAAAACTCAAAAACTATTGTGGATAAGATTTTTCCTTTTCTTTTTTCTTAAAAATTAATTAAAAAAGAATCCCCAAACTCCCTCCCACCACTGCCCCCTCCACAGCTCAAAGCTATGGCTGGGCTCTCCGGCAGTTTCCCTTTCAAATCTCTCTTATCCCAATCCTCCGCCGCTGCCACCACCACCTCCTCCTCCTCCGCCGCACCTCCCCCAACCTCCTCCTCTTCCACTACTCCCTTCCGAACCTCATTCCCCTCTCTTCCCATCTCCTCCTCTAATTCCAATGCTTTCAAATTCCGCCTTCACAAATCCAACTCTCTACGTCTCCTCTTTTCCTCTCCCGTTTCCTCTCCTATTCGTACTTCTGTATTTGCTCGACTCGGCGGCGCTCCTCGATCTTCTGGACCGCCTGATTCTAAGCGCTCTAACGATGAAATTGGTCTCGATATTTCCGCCATCAAGTTTGTGTCCTGTACTTGTACTAGTATGATTTATGGATTTTTTTTATATATTTGGAGTTCTTAATTGTTTGGTTGCGTTTTTTGTGATTTATTTGTACAGGTCAATTAATGTGAGGCTTATCGATGCTGAACAGAATATGGTAGGTGTATGGTTTGTGCTTTGGGAAATATATATGTGTGTGTGTGTGTGTGTTTGTCTGTTTCCTTTTGCTACAATGCAATACTTGTATGCTAGGTGTGGTAGTTTGAAACGTTCATGTTCTTATAATGAATAGGTTGGAGTTGTGTCGAAGGCCCAAGCCATTCAAATGGCTGAAGATGCTGAACTTGATTTGGTATGTCTTCTTGCATCGTTTGTTGTTAAAATAATTGTTTGTCTATGAATTCCAAAGAACCTTGCACCAAAATTTAATCGCCTTTTGAGGAAACATTGTATAGTTCATATTTCTTTTACTAAAAATGGAATTAAAAGCATATAATGATAAACTATTCGAAAAATTATAGATATAAGTTATAGCCTTTCATCCATAGCTAGAAGAACCATCGCATAGTGAATGGATTTCATTTCACAGCTGTGCATTAGGCCAATTTTACTGTTTGCTCTGATATAAAATTGTAATTTCCTTCTCCTATTTGTCAACACGAGACTTGGATAAATGCTGGATAATGCCTTGTTATTAAGATATATGATTAAAAATTTCTTAGAACCTTTGAGTTGTCTGGATGCTCCTGGTGCAATAAACGATGCTCATTTCACATTCTGTTTTTTACTTCTTAACTTGGATAAAGAAGGTCGGGGGCTAATATTTTTCCTGGAATCCTAATATTTTCGTTGCTTTATGTAAGGTTTAGCTAATTCTTTTTATTCCGTTCAGGTTGTAGTATCCCCTGATGCAGATCCTCCAGTTGTTAGAATAATGGATTACAAGTATGCCACTTTCTCTCTCTGGTTTCCCCATTCCCTCTATGCTTGTGGATAAGGTTGTATTTTGCTTGAGTTCAATGCCAATGTGATCAACTTTTATGATTAATTTCAATTCTTATGTTAAACATGTTGATGATATATTTTTACATTAATACTTTACTTTTAATTAAATGTTTCCGAGAAAGTATGGAAATATGGTGCAGTATAACGGTGACATAATGAAATAGGATGATTGTTATGTTGTTTTCACTGGGAGAATTGGAAGGCCACTTTTTTTTTTCCTTATTACTATTTTGTTACTTTTATGTTGAGAATTTTCTTTTCCCGTCTTTTGTTTCTCAGTAAATATAGGTATGAACTGCAAAAAAAGAAAAGAGGACAACAGAAGAAAAGTGCTGGTGAATCTCTTTTTTAATAGAGTCTGCATATTTTCTTTGGTACACAAATCTTTCAGATTTGTGGAAAGATACTGACAAAGTGTGGTTTTGAATGTTAGCAACTCGCATGGATTTGAAAGAGCTTAAAATGGGGTAACACATTCCTTCATCTCTCTGTCTCTTTTCTCTCTGGAGGTTTCCTTTTTTTTTCTTCTTTTTCTCGACTTAAAGCCACTTGAACTTGTGGCGTCTAAGATCAATAATCACCCAGCCTGCAAAAGCAAAGAGACATGAGAGACCTTTGTCTGCTCTTTCAGAAACATTTATGCACATGTTCCTTTTCTCAATCTCGACCTTCTGGTTGTTATTATTGTTGCACAGTTATAACATCGACCAACATGATTACTCTGTCCGTTTGAAAGCTGCGAAGAAATTTTTAAAAGACGGTGACAAGGTTTCTTATCACATCTTCTATATCTTTCATCTTCTTTTCATGCATCCAGATATATCGTAATAGTTTGTCTGAATCACTTTGGAAGTCTTTTATCGAATATTGTAGGTTAAAGTTATAGTGAACCTGAAAGGTCGTGAAAATGAGTTCAGAAACATAGCAATTGAGCTCATCAGACGTTTCCAGGAAGAAGTTGGGGAGGTATTTATGTTGTATCAATTTTGTCTCGACTTCAATATCTGTTCATCACGGCAATACTAGAGAAAATGATTGCCCATTGTCCCTTATTGTGGTCTTGTTACTCAATACTGCAAATAATGGTTGTTTTATAGAACCATTTTTTTTAAAAGAATGCTTAGAACCTAAAACATTGTGATCATATTGCTTGTTTGTCTTGTTTCTTTCCTATTTGTGTCTGCTAATCTCTCTTTATCCCATATCCGTGCTTTGGGTTAATACCTGAAACCAAGTCTTACCTTCTCTTTATTTATCTCTCTTCCCCATTGACGTTCTCGTTATTTTATTATACACAGCTTGCAACTGAAGAGGCCAAGAACTTCAGAGACAGGAATATATTTATTGTTCTAGTCCCTAACAAGGCTGTTCTTCAAAAGGCTCAAGAACCTCCCAAGAAAAAAGATAAACCTGCAGTTAATGGAGTTTCTGCCAGTGTTTAGTCAAGCTTTCGCCAAAAATAGATGGGTTAGTTGCAACATTCTAAATCTTCTTCTACCATAGAGAAAATTTGAGAGATTAAATGTATAATGTTTTTGTTTAATCTTATGCATCAAACTGTTGTTCGTTCTATTTTTTTAGTGGAATGTTTAGAAATCAGATGTGTTACTAATTCTTTAGAAAACATCAATTCATATAACCATCCTTTGCT

mRNA sequence

AGGAATATAGAGTTAGAGTGATGAGTCGACTCCAAAACTCAAAAACTATTGTGGATAAGATTTTTCCTTTTCTTTTTTCTTAAAAATTAATTAAAAAAGAATCCCCAAACTCCCTCCCACCACTGCCCCCTCCACAGCTCAAAGCTATGGCTGGGCTCTCCGGCAGTTTCCCTTTCAAATCTCTCTTATCCCAATCCTCCGCCGCTGCCACCACCACCTCCTCCTCCTCCGCCGCACCTCCCCCAACCTCCTCCTCTTCCACTACTCCCTTCCGAACCTCATTCCCCTCTCTTCCCATCTCCTCCTCTAATTCCAATGCTTTCAAATTCCGCCTTCACAAATCCAACTCTCTACGTCTCCTCTTTTCCTCTCCCGTTTCCTCTCCTATTCGTACTTCTGTATTTGCTCGACTCGGCGGCGCTCCTCGATCTTCTGGACCGCCTGATTCTAAGCGCTCTAACGATGAAATTGGTCTCGATATTTCCGCCATCAAGTCAATTAATGTGAGGCTTATCGATGCTGAACAGAATATGGTTGGAGTTGTGTCGAAGGCCCAAGCCATTCAAATGGCTGAAGATGCTGAACTTGATTTGGTTGTAGTATCCCCTGATGCAGATCCTCCAGTTGTTAGAATAATGGATTACAATAAATATAGGTATGAACTGCAAAAAAAGAAAAGAGGACAACAGAAGAAAAGTGCTGCAACTCGCATGGATTTGAAAGAGCTTAAAATGGGTTATAACATCGACCAACATGATTACTCTGTCCGTTTGAAAGCTGCGAAGAAATTTTTAAAAGACGGTGACAAGGTTAAAGTTATAGTGAACCTGAAAGGTCGTGAAAATGAGTTCAGAAACATAGCAATTGAGCTCATCAGACGTTTCCAGGAAGAAGTTGGGGAGCTTGCAACTGAAGAGGCCAAGAACTTCAGAGACAGGAATATATTTATTGTTCTAGTCCCTAACAAGGCTGTTCTTCAAAAGGCTCAAGAACCTCCCAAGAAAAAAGATAAACCTGCAGTTAATGGAGTTTCTGCCAGTGTTTAGTCAAGCTTTCGCCAAAAATAGATGGGTTAGTTGCAACATTCTAAATCTTCTTCTACCATAGAGAAAATTTGAGAGATTAAATGTATAATGTTTTTGTTTAATCTTATGCATCAAACTGTTGTTCGTTCTATTTTTTTAGTGGAATGTTTAGAAATCAGATGTGTTACTAATTCTTTAGAAAACATCAATTCATATAACCATCCTTTGCT

Coding sequence (CDS)

ATGGCTGGGCTCTCCGGCAGTTTCCCTTTCAAATCTCTCTTATCCCAATCCTCCGCCGCTGCCACCACCACCTCCTCCTCCTCCGCCGCACCTCCCCCAACCTCCTCCTCTTCCACTACTCCCTTCCGAACCTCATTCCCCTCTCTTCCCATCTCCTCCTCTAATTCCAATGCTTTCAAATTCCGCCTTCACAAATCCAACTCTCTACGTCTCCTCTTTTCCTCTCCCGTTTCCTCTCCTATTCGTACTTCTGTATTTGCTCGACTCGGCGGCGCTCCTCGATCTTCTGGACCGCCTGATTCTAAGCGCTCTAACGATGAAATTGGTCTCGATATTTCCGCCATCAAGTCAATTAATGTGAGGCTTATCGATGCTGAACAGAATATGGTTGGAGTTGTGTCGAAGGCCCAAGCCATTCAAATGGCTGAAGATGCTGAACTTGATTTGGTTGTAGTATCCCCTGATGCAGATCCTCCAGTTGTTAGAATAATGGATTACAATAAATATAGGTATGAACTGCAAAAAAAGAAAAGAGGACAACAGAAGAAAAGTGCTGCAACTCGCATGGATTTGAAAGAGCTTAAAATGGGTTATAACATCGACCAACATGATTACTCTGTCCGTTTGAAAGCTGCGAAGAAATTTTTAAAAGACGGTGACAAGGTTAAAGTTATAGTGAACCTGAAAGGTCGTGAAAATGAGTTCAGAAACATAGCAATTGAGCTCATCAGACGTTTCCAGGAAGAAGTTGGGGAGCTTGCAACTGAAGAGGCCAAGAACTTCAGAGACAGGAATATATTTATTGTTCTAGTCCCTAACAAGGCTGTTCTTCAAAAGGCTCAAGAACCTCCCAAGAAAAAAGATAAACCTGCAGTTAATGGAGTTTCTGCCAGTGTTTAG

Protein sequence

MAGLSGSFPFKSLLSQSSAAATTTSSSSAAPPPTSSSSTTPFRTSFPSLPISSSNSNAFKFRLHKSNSLRLLFSSPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEIGLDISAIKSINVRLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNGVSASV*
Homology
BLAST of CSPI02G05270 vs. ExPASy Swiss-Prot
Match: Q94B52 (Translation initiation factor IF3-4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IF3-4 PE=2 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 8.7e-77
Identity = 154/214 (71.96%), Postives = 182/214 (85.05%), Query Frame = 0

Query: 90  GGAPRSSGPPD----SKRSNDEIGLDISAIKSINVRLIDAEQNMVGVVSKAQAIQMAEDA 149
           GG   S  P D     K S D+  LDISAI+S  VRLID +QNM+G+VSK +A++ AEDA
Sbjct: 62  GGGGGSRFPGDRRGRQKESEDDDSLDISAIRSATVRLIDDQQNMIGLVSKEEAVRRAEDA 121

Query: 150 ELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHDY 209
           ELDLV++SPDADPPVVR+MDY+KYRYE QK+K+ QQKK+  TRMDLKELKMGYNIDQHDY
Sbjct: 122 ELDLVILSPDADPPVVRMMDYSKYRYEQQKRKKEQQKKT--TRMDLKELKMGYNIDQHDY 181

Query: 210 SVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQEEVGELATEEAKNFRDRN 269
           SVR++AA+KFL+DGDKVKVIVN+KGRENEFRNIAIEL+RRFQ E+GEL TEE+KNFRDRN
Sbjct: 182 SVRMRAARKFLQDGDKVKVIVNMKGRENEFRNIAIELLRRFQTEIGELGTEESKNFRDRN 241

Query: 270 IFIVLVPNKAVLQKAQE-PPKKKDKPAVNGVSAS 299
           +FIVLVPNK V++K QE PPKKK KPA + VSA+
Sbjct: 242 LFIVLVPNKEVIRKVQEPPPKKKKKPADDKVSAA 273

BLAST of CSPI02G05270 vs. ExPASy Swiss-Prot
Match: O82234 (Translation initiation factor IF3-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IF3-2 PE=2 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 4.5e-73
Identity = 163/275 (59.27%), Postives = 205/275 (74.55%), Query Frame = 0

Query: 36  SSSTTPFRTSFPSLPISSSNSNAF----KFRLHKSNSLRLLFSSPVSSPIR----TSVFA 95
           +SST  F   F  +  + S+ + F    K    + +   L F++   SP R    T  F 
Sbjct: 5   TSSTVGFNAVFTGITKTVSSHSLFSVDSKLCSLRLSKTELSFTNLTPSPRRAFAVTCRFG 64

Query: 96  RLGGAPRSSGPPDSKRSND---EIGLDISAIKSINVRLIDAEQNMVGVVSKAQAIQMAED 155
             GG  R SG     R  +   +  LDIS+I+S  VRLID +QNM+G+VSK +A++MA+D
Sbjct: 65  GGGGGYRFSGDNRRGRPKEAEIDEALDISSIRSATVRLIDGQQNMLGLVSKDEAVRMADD 124

Query: 156 AELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHD 215
           AELDLV++SPDADPPVV++MDY+KYRYE QK+K+ QQKK+  TRMDLKELKMGYNIDQHD
Sbjct: 125 AELDLVILSPDADPPVVKMMDYSKYRYEQQKRKKDQQKKT--TRMDLKELKMGYNIDQHD 184

Query: 216 YSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQEEVGELATEEAKNFRDR 275
           YSVRL+AA+KFL+DGDKVKVIV++KGRENEFRNIAIEL+RRFQ E+GELATEE+KNFRDR
Sbjct: 185 YSVRLRAAQKFLQDGDKVKVIVSMKGRENEFRNIAIELLRRFQTEIGELATEESKNFRDR 244

Query: 276 NIFIVLVPNKAVLQKAQEPP-KKKDKPAVNGVSAS 299
           N+FI+LVPNK +++K QEPP +KK K A N  SAS
Sbjct: 245 NMFIILVPNKEMIRKPQEPPTRKKKKTAENEASAS 277

BLAST of CSPI02G05270 vs. ExPASy Swiss-Prot
Match: B0JKQ1 (Translation initiation factor IF-3 OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=infC PE=3 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 5.5e-31
Identity = 72/176 (40.91%), Postives = 118/176 (67.05%), Query Frame = 0

Query: 100 DSKRSNDEIGLDISAIKSINVRLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPP 159
           D +R+  ++      I+   +R+ID++ + +G+++ A+A+++AE+ ELDLV+VS  A PP
Sbjct: 3   DKRRTTRDLPQINERIRFPEIRVIDSDGSQLGIITPAEALRVAEEKELDLVLVSETASPP 62

Query: 160 VVRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDG 219
           V RIMDY KY++E +KK R  +KK      D+KE+KM Y ID+HDY+VR+  A++FLK G
Sbjct: 63  VCRIMDYGKYKFEQEKKAREAKKKQHTA--DIKEVKMRYKIDEHDYNVRVNQAQRFLKAG 122

Query: 220 DKVKVIVNLKGRENEFRNIAIELIRRFQEEVGELA-TEEAKNFRDRNIFIVLVPNK 275
           DKVK  +  +GRE +  N+A EL+ R  +++ ++A  ++A     RN+ ++L P K
Sbjct: 123 DKVKATITFRGREIQHSNLAEELLARMAKDLQDVAEVQQAPKKEGRNMMMMLSPKK 176

BLAST of CSPI02G05270 vs. ExPASy Swiss-Prot
Match: Q0IDZ5 (Translation initiation factor IF-3 OS=Synechococcus sp. (strain CC9311) OX=64471 GN=infC PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 2.7e-30
Identity = 79/175 (45.14%), Postives = 115/175 (65.71%), Query Frame = 0

Query: 120 VRLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRG 179
           +R++DA+   +GV+ + QA+++A + ELDLV+VS  ADPPV RIMDY K+++E +KK + 
Sbjct: 28  LRVVDADGEQLGVIDREQALEVARERELDLVLVSEKADPPVCRIMDYGKFKFEQEKKAKE 87

Query: 180 QQKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIA 239
            +KKS  T  ++KE+KM Y IDQHDY VR+  A++FLK GDKVK  V  +GRE +   +A
Sbjct: 88  AKKKSHQT--EVKEVKMRYKIDQHDYDVRIGQAQRFLKAGDKVKCTVIFRGREIQHTALA 147

Query: 240 IELIRRFQEEVGELATEEAKNFRD-RNIFIVLVPNKAVLQKAQEPPKKKDKPAVN 294
             L+RR  +++ E A  +    R+ RN+ + L P KA L K     K KD+  VN
Sbjct: 148 EVLLRRMAKDLEEPAEVQQPPKREGRNMIMFLTPRKAPLVK-----KDKDEEVVN 195

BLAST of CSPI02G05270 vs. ExPASy Swiss-Prot
Match: A5GI06 (Translation initiation factor IF-3 OS=Synechococcus sp. (strain WH7803) OX=32051 GN=infC PE=3 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 3.6e-30
Identity = 77/177 (43.50%), Postives = 116/177 (65.54%), Query Frame = 0

Query: 120 VRLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRG 179
           +R++DA+   +GV+ + +A+++A++ ELDLV+VS  ADPPV RIMDY KY++E +KK + 
Sbjct: 28  LRVVDADGTQLGVIDREKALEVAQERELDLVLVSEKADPPVCRIMDYGKYKFEQEKKAKE 87

Query: 180 QQKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIA 239
            +KKS  T  ++KE+KM Y IDQHDY VR+  A++FLK GDKVK  V  +GRE +   +A
Sbjct: 88  AKKKSHQT--EVKEVKMRYKIDQHDYDVRIGQAQRFLKAGDKVKCTVIFRGREIQHTALA 147

Query: 240 IELIRRFQEEVGELATEEAKNFRD-RNIFIVLVPNKAVLQKAQEPPKKKDKPAVNGV 296
             L+RR  +++ E A  +    R+ RN+ + L P K  L K  +    K++PA   V
Sbjct: 148 ETLLRRMAKDLEEPAEIQQPPKREGRNMIMFLTPRKTPLVKKDD----KEEPATRAV 198

BLAST of CSPI02G05270 vs. ExPASy TrEMBL
Match: A0A0A0LH34 (Translation initiation factor IF-3 OS=Cucumis sativus OX=3659 GN=Csa_2G036070 PE=3 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 6.5e-152
Identity = 299/299 (100.00%), Postives = 299/299 (100.00%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTTSSSSAAPPPTSSSSTTPFRTSFPSLPISSSNSNAFK 60
           MAGLSGSFPFKSLLSQSSAAATTTSSSSAAPPPTSSSSTTPFRTSFPSLPISSSNSNAFK
Sbjct: 1   MAGLSGSFPFKSLLSQSSAAATTTSSSSAAPPPTSSSSTTPFRTSFPSLPISSSNSNAFK 60

Query: 61  FRLHKSNSLRLLFSSPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEIGLDISAIKSINV 120
           FRLHKSNSLRLLFSSPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEIGLDISAIKSINV
Sbjct: 61  FRLHKSNSLRLLFSSPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEIGLDISAIKSINV 120

Query: 121 RLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ 180
           RLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ
Sbjct: 121 RLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ 180

Query: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240
           QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI
Sbjct: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240

Query: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNGVSASV 300
           ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNGVSASV
Sbjct: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNGVSASV 299

BLAST of CSPI02G05270 vs. ExPASy TrEMBL
Match: A0A1S3BV82 (Translation initiation factor IF-3 OS=Cucumis melo OX=3656 GN=LOC103493630 PE=3 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 4.9e-139
Identity = 280/300 (93.33%), Postives = 288/300 (96.00%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTTSSSSAAPPPTSSSSTTPFRTSFPSLPISSS-NSNAF 60
           MAGLSGSFPFKSLLSQSSAA+TTTS ++A   PTSSSSTTPFRTS PSLPISSS NSNAF
Sbjct: 1   MAGLSGSFPFKSLLSQSSAASTTTSPAAA---PTSSSSTTPFRTSTPSLPISSSFNSNAF 60

Query: 61  KFRLHKSNSLRLLFSSPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEIGLDISAIKSIN 120
           K RLHKSNSLRL+FS PVSSPIRTSVFARLGGAPRSSGPPDSKRSNDE GLD+SAI+S N
Sbjct: 61  KLRLHKSNSLRLVFSFPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEFGLDVSAIRSNN 120

Query: 121 VRLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRG 180
           VRLIDA+QNMVGVVSKAQAIQMAEDAELDLVV+SPDADPPVVRIMDYNKYRYELQKKKRG
Sbjct: 121 VRLIDAQQNMVGVVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRG 180

Query: 181 QQKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIA 240
           QQKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIA
Sbjct: 181 QQKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIA 240

Query: 241 IELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNGVSASV 300
           IELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVN VSA V
Sbjct: 241 IELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNEVSAGV 297

BLAST of CSPI02G05270 vs. ExPASy TrEMBL
Match: A0A6J1DYM5 (Translation initiation factor IF-3 OS=Momordica charantia OX=3673 GN=LOC111024717 PE=3 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 1.9e-122
Identity = 248/299 (82.94%), Postives = 269/299 (89.97%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTTSSSSAAPPPTSSSSTTPFRTSFPSLPISSSNSNAFK 60
           MAGL+  FPFK LLSQSSA    T+SS+AA   +S+++  PFRTS   LP+SSS SNAF 
Sbjct: 1   MAGLTSRFPFKPLLSQSSA----TTSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFG 60

Query: 61  FRLHKSNSLRLLFSSPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEIGLDISAIKSINV 120
           FRLH SNSLRL+    VSSPIR SVFAR GGAPRSSGP DSKRS+D+ GLD+SAI+S NV
Sbjct: 61  FRLHNSNSLRLI----VSSPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNV 120

Query: 121 RLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ 180
           RLIDA+QNMVG+VSKAQAIQMAEDAELDLVV+SPDADPPVVRIMDYNKYRYELQKKKRGQ
Sbjct: 121 RLIDAQQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQ 180

Query: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240
           QKKSAATRMDLKELKMGYNIDQHDYSVRLKAA++FLKDGDKVKVIVNLKGRENEFRNIAI
Sbjct: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAI 240

Query: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNGVSASV 300
           ELIRRFQ+E+GELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKD PA+N VSA V
Sbjct: 241 ELIRRFQDEIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDNPAINEVSAGV 291

BLAST of CSPI02G05270 vs. ExPASy TrEMBL
Match: A0A6J1G3Y5 (Translation initiation factor IF-3 OS=Cucurbita moschata OX=3662 GN=LOC111450575 PE=3 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 9.9e-116
Identity = 239/300 (79.67%), Postives = 262/300 (87.33%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTTSSSSAAPPPTSSSSTTPFRTSFPSLPISSSNSNAFK 60
           MAGLSG FPFKS+ SQSSA AT T+++S      SSSS TPFRTS   LP+ SS SNAF+
Sbjct: 1   MAGLSGRFPFKSISSQSSATATATTTTS------SSSSATPFRTSIFPLPLPSSKSNAFR 60

Query: 61  FRLHKSNSLRLLFSSPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEIGLDISAIKSINV 120
           FRLH SNSLRL     VSSPIRTSVFAR GGAPRSSGP  SKRS D + LD+S+I+S NV
Sbjct: 61  FRLHNSNSLRL-----VSSPIRTSVFARYGGAPRSSGPAGSKRSEDSLALDVSSIRSNNV 120

Query: 121 RLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ 180
           RLID +QNMVG+V K+QAIQMAEDAELDLVV+SPDADPPVVRIMDY+KYRY+LQKKKR Q
Sbjct: 121 RLIDEQQNMVGIVPKSQAIQMAEDAELDLVVLSPDADPPVVRIMDYSKYRYDLQKKKREQ 180

Query: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240
           QKKSAATRMDLKELKMGY IDQHDYSVRL+AAKKFL+DGDKVKVIVNLKGRENEFR IAI
Sbjct: 181 QKKSAATRMDLKELKMGYKIDQHDYSVRLRAAKKFLQDGDKVKVIVNLKGRENEFRKIAI 240

Query: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKP-AVNGVSASV 300
           EL++RFQEE+GELATEEAKNFRDRN+FIVL PNKAVLQK+QEPPKKKDKP AVN VSA V
Sbjct: 241 ELVKRFQEELGELATEEAKNFRDRNVFIVLAPNKAVLQKSQEPPKKKDKPAAVNEVSARV 289

BLAST of CSPI02G05270 vs. ExPASy TrEMBL
Match: A0A6J1KDY9 (Translation initiation factor IF-3 OS=Cucurbita maxima OX=3661 GN=LOC111493999 PE=3 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 1.1e-114
Identity = 238/300 (79.33%), Postives = 258/300 (86.00%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTTSSSSAAPPPTSSSSTTPFRTSFPSLPISSSNSNAFK 60
           MAGLSG FPFKS+ SQSSA AT T+S        SSSS TPFRTS   LP  SS SNA +
Sbjct: 1   MAGLSGRFPFKSISSQSSATATATTS--------SSSSATPFRTSIFPLPFPSSKSNAVR 60

Query: 61  FRLHKSNSLRLLFSSPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEIGLDISAIKSINV 120
           FRLH SNSLRL     VSSPIRTSVFAR GGAPRSSGP  SKRS D + LD+S+I+S NV
Sbjct: 61  FRLHSSNSLRL-----VSSPIRTSVFARYGGAPRSSGPTGSKRSEDSLALDVSSIRSNNV 120

Query: 121 RLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ 180
           RLID +QNMVG+V K+QAIQMAEDAELDLVV+SPDADPPVVRIMDY+KYRY+LQKKKR Q
Sbjct: 121 RLIDEQQNMVGIVPKSQAIQMAEDAELDLVVLSPDADPPVVRIMDYSKYRYDLQKKKREQ 180

Query: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240
           QKKSAATRMDLKELKMGY IDQHDYSVRL+AAKKFL+DGDKVKVIVNLKGRENEFR IAI
Sbjct: 181 QKKSAATRMDLKELKMGYKIDQHDYSVRLRAAKKFLQDGDKVKVIVNLKGRENEFRKIAI 240

Query: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKP-AVNGVSASV 300
           EL++RFQEE+GELATEEAKNFRDRN+FIVL PNKAVLQK+QEPPKKKDKP AVN VSA V
Sbjct: 241 ELVKRFQEELGELATEEAKNFRDRNVFIVLAPNKAVLQKSQEPPKKKDKPAAVNEVSAGV 287

BLAST of CSPI02G05270 vs. NCBI nr
Match: XP_004144726.1 (translation initiation factor IF3-4, chloroplastic [Cucumis sativus] >KGN61058.1 hypothetical protein Csa_021304 [Cucumis sativus])

HSP 1 Score: 546.6 bits (1407), Expect = 1.4e-151
Identity = 299/299 (100.00%), Postives = 299/299 (100.00%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTTSSSSAAPPPTSSSSTTPFRTSFPSLPISSSNSNAFK 60
           MAGLSGSFPFKSLLSQSSAAATTTSSSSAAPPPTSSSSTTPFRTSFPSLPISSSNSNAFK
Sbjct: 1   MAGLSGSFPFKSLLSQSSAAATTTSSSSAAPPPTSSSSTTPFRTSFPSLPISSSNSNAFK 60

Query: 61  FRLHKSNSLRLLFSSPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEIGLDISAIKSINV 120
           FRLHKSNSLRLLFSSPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEIGLDISAIKSINV
Sbjct: 61  FRLHKSNSLRLLFSSPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEIGLDISAIKSINV 120

Query: 121 RLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ 180
           RLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ
Sbjct: 121 RLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ 180

Query: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240
           QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI
Sbjct: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240

Query: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNGVSASV 300
           ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNGVSASV
Sbjct: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNGVSASV 299

BLAST of CSPI02G05270 vs. NCBI nr
Match: XP_008452687.1 (PREDICTED: translation initiation factor IF-3 [Cucumis melo])

HSP 1 Score: 503.8 bits (1296), Expect = 1.0e-138
Identity = 280/300 (93.33%), Postives = 288/300 (96.00%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTTSSSSAAPPPTSSSSTTPFRTSFPSLPISSS-NSNAF 60
           MAGLSGSFPFKSLLSQSSAA+TTTS ++A   PTSSSSTTPFRTS PSLPISSS NSNAF
Sbjct: 1   MAGLSGSFPFKSLLSQSSAASTTTSPAAA---PTSSSSTTPFRTSTPSLPISSSFNSNAF 60

Query: 61  KFRLHKSNSLRLLFSSPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEIGLDISAIKSIN 120
           K RLHKSNSLRL+FS PVSSPIRTSVFARLGGAPRSSGPPDSKRSNDE GLD+SAI+S N
Sbjct: 61  KLRLHKSNSLRLVFSFPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEFGLDVSAIRSNN 120

Query: 121 VRLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRG 180
           VRLIDA+QNMVGVVSKAQAIQMAEDAELDLVV+SPDADPPVVRIMDYNKYRYELQKKKRG
Sbjct: 121 VRLIDAQQNMVGVVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRG 180

Query: 181 QQKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIA 240
           QQKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIA
Sbjct: 181 QQKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIA 240

Query: 241 IELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNGVSASV 300
           IELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVN VSA V
Sbjct: 241 IELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNEVSAGV 297

BLAST of CSPI02G05270 vs. NCBI nr
Match: XP_038891171.1 (translation initiation factor IF3-4, chloroplastic [Benincasa hispida])

HSP 1 Score: 464.9 bits (1195), Expect = 5.2e-127
Identity = 260/300 (86.67%), Postives = 277/300 (92.33%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTTSSSSAAPPPTSSSSTTPFRTSFPSLPISSSNSNAFK 60
           MAGLSG FPFK LLSQS+  ATTTS+++A    TSSSS TPFRTS   LP+SSS +NAFK
Sbjct: 1   MAGLSGRFPFKPLLSQST-TATTTSAAAA----TSSSSATPFRTSIRPLPLSSSKANAFK 60

Query: 61  FRLHKSNSLRLLFSSPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEIGLDISAIKSINV 120
           FRL KSNSLRL+ S PVSSPIRTSVF+R GGAPRSSGP DSKRS+DE GLD+SAI+S NV
Sbjct: 61  FRLRKSNSLRLVVSFPVSSPIRTSVFSRYGGAPRSSGPADSKRSDDEFGLDVSAIRSNNV 120

Query: 121 RLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ 180
           RLIDA+QNMVG+VSKAQAIQMAEDAELDLVV+SPDADPPVVRIMDYNKYRYELQKKKRGQ
Sbjct: 121 RLIDAQQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQ 180

Query: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240
           QKKSAATRMDLKELKMGYNIDQHDYSVRL+AA+KFLKDGDKVKVIVNLKGRENEFRNIAI
Sbjct: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLRAAQKFLKDGDKVKVIVNLKGRENEFRNIAI 240

Query: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQE-PPKKKDKPAVNGVSASV 300
           ELIRRFQEE+GELATEEAKNFRDRNIFIVLVPNKAVLQKAQE PPKKKDKPAVN VSA V
Sbjct: 241 ELIRRFQEEIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEQPPKKKDKPAVNEVSAGV 295

BLAST of CSPI02G05270 vs. NCBI nr
Match: XP_022158178.1 (translation initiation factor IF3-4, chloroplastic [Momordica charantia] >XP_022158179.1 translation initiation factor IF3-4, chloroplastic [Momordica charantia])

HSP 1 Score: 448.7 bits (1153), Expect = 3.8e-122
Identity = 248/299 (82.94%), Postives = 269/299 (89.97%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTTSSSSAAPPPTSSSSTTPFRTSFPSLPISSSNSNAFK 60
           MAGL+  FPFK LLSQSSA    T+SS+AA   +S+++  PFRTS   LP+SSS SNAF 
Sbjct: 1   MAGLTSRFPFKPLLSQSSA----TTSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFG 60

Query: 61  FRLHKSNSLRLLFSSPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEIGLDISAIKSINV 120
           FRLH SNSLRL+    VSSPIR SVFAR GGAPRSSGP DSKRS+D+ GLD+SAI+S NV
Sbjct: 61  FRLHNSNSLRLI----VSSPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNV 120

Query: 121 RLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ 180
           RLIDA+QNMVG+VSKAQAIQMAEDAELDLVV+SPDADPPVVRIMDYNKYRYELQKKKRGQ
Sbjct: 121 RLIDAQQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQ 180

Query: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240
           QKKSAATRMDLKELKMGYNIDQHDYSVRLKAA++FLKDGDKVKVIVNLKGRENEFRNIAI
Sbjct: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAI 240

Query: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNGVSASV 300
           ELIRRFQ+E+GELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKD PA+N VSA V
Sbjct: 241 ELIRRFQDEIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDNPAINEVSAGV 291

BLAST of CSPI02G05270 vs. NCBI nr
Match: XP_023547209.1 (translation initiation factor IF3-4, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 426.8 bits (1096), Expect = 1.6e-115
Identity = 239/300 (79.67%), Postives = 261/300 (87.00%), Query Frame = 0

Query: 1   MAGLSGSFPFKSLLSQSSAAATTTSSSSAAPPPTSSSSTTPFRTSFPSLPISSSNSNAFK 60
           MAGLSG FPFKS+ SQSSA AT T+S        SSSS TPFRTS   LP+ SS SNAF+
Sbjct: 1   MAGLSGRFPFKSISSQSSATATATTS--------SSSSATPFRTSIFPLPLPSSKSNAFR 60

Query: 61  FRLHKSNSLRLLFSSPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEIGLDISAIKSINV 120
           FRLH SNSLRL     VSSPIRTSVFAR GGAPRSSGP  SKRS D + LD+S+I+S NV
Sbjct: 61  FRLHNSNSLRL-----VSSPIRTSVFARYGGAPRSSGPTGSKRSEDSLALDVSSIRSNNV 120

Query: 121 RLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ 180
           RLID +QNMVG+V K+QAIQMAEDAELDLVV+SPDADPPVVRIMDY+KYRY+LQKKKR Q
Sbjct: 121 RLIDEQQNMVGIVPKSQAIQMAEDAELDLVVLSPDADPPVVRIMDYSKYRYDLQKKKREQ 180

Query: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240
           QKKSAATRMDLKELKMGY IDQHDYSVRL+AAKKFL+DGDKVKVIVNLKGRENEFR IAI
Sbjct: 181 QKKSAATRMDLKELKMGYKIDQHDYSVRLRAAKKFLQDGDKVKVIVNLKGRENEFRKIAI 240

Query: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKP-AVNGVSASV 300
           EL++RFQEE+GELATEEAKNFRDRN+FIVL PNKAVLQK+QEPPKKKDKP AVN VSA+V
Sbjct: 241 ELVKRFQEELGELATEEAKNFRDRNVFIVLAPNKAVLQKSQEPPKKKDKPAAVNEVSAAV 287

BLAST of CSPI02G05270 vs. TAIR 10
Match: AT4G30690.1 (Translation initiation factor 3 protein )

HSP 1 Score: 288.5 bits (737), Expect = 6.2e-78
Identity = 154/214 (71.96%), Postives = 182/214 (85.05%), Query Frame = 0

Query: 90  GGAPRSSGPPD----SKRSNDEIGLDISAIKSINVRLIDAEQNMVGVVSKAQAIQMAEDA 149
           GG   S  P D     K S D+  LDISAI+S  VRLID +QNM+G+VSK +A++ AEDA
Sbjct: 62  GGGGGSRFPGDRRGRQKESEDDDSLDISAIRSATVRLIDDQQNMIGLVSKEEAVRRAEDA 121

Query: 150 ELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHDY 209
           ELDLV++SPDADPPVVR+MDY+KYRYE QK+K+ QQKK+  TRMDLKELKMGYNIDQHDY
Sbjct: 122 ELDLVILSPDADPPVVRMMDYSKYRYEQQKRKKEQQKKT--TRMDLKELKMGYNIDQHDY 181

Query: 210 SVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQEEVGELATEEAKNFRDRN 269
           SVR++AA+KFL+DGDKVKVIVN+KGRENEFRNIAIEL+RRFQ E+GEL TEE+KNFRDRN
Sbjct: 182 SVRMRAARKFLQDGDKVKVIVNMKGRENEFRNIAIELLRRFQTEIGELGTEESKNFRDRN 241

Query: 270 IFIVLVPNKAVLQKAQE-PPKKKDKPAVNGVSAS 299
           +FIVLVPNK V++K QE PPKKK KPA + VSA+
Sbjct: 242 LFIVLVPNKEVIRKVQEPPPKKKKKPADDKVSAA 273

BLAST of CSPI02G05270 vs. TAIR 10
Match: AT2G24060.1 (Translation initiation factor 3 protein )

HSP 1 Score: 276.2 bits (705), Expect = 3.2e-74
Identity = 163/275 (59.27%), Postives = 205/275 (74.55%), Query Frame = 0

Query: 36  SSSTTPFRTSFPSLPISSSNSNAF----KFRLHKSNSLRLLFSSPVSSPIR----TSVFA 95
           +SST  F   F  +  + S+ + F    K    + +   L F++   SP R    T  F 
Sbjct: 5   TSSTVGFNAVFTGITKTVSSHSLFSVDSKLCSLRLSKTELSFTNLTPSPRRAFAVTCRFG 64

Query: 96  RLGGAPRSSGPPDSKRSND---EIGLDISAIKSINVRLIDAEQNMVGVVSKAQAIQMAED 155
             GG  R SG     R  +   +  LDIS+I+S  VRLID +QNM+G+VSK +A++MA+D
Sbjct: 65  GGGGGYRFSGDNRRGRPKEAEIDEALDISSIRSATVRLIDGQQNMLGLVSKDEAVRMADD 124

Query: 156 AELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHD 215
           AELDLV++SPDADPPVV++MDY+KYRYE QK+K+ QQKK+  TRMDLKELKMGYNIDQHD
Sbjct: 125 AELDLVILSPDADPPVVKMMDYSKYRYEQQKRKKDQQKKT--TRMDLKELKMGYNIDQHD 184

Query: 216 YSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQEEVGELATEEAKNFRDR 275
           YSVRL+AA+KFL+DGDKVKVIV++KGRENEFRNIAIEL+RRFQ E+GELATEE+KNFRDR
Sbjct: 185 YSVRLRAAQKFLQDGDKVKVIVSMKGRENEFRNIAIELLRRFQTEIGELATEESKNFRDR 244

Query: 276 NIFIVLVPNKAVLQKAQEPP-KKKDKPAVNGVSAS 299
           N+FI+LVPNK +++K QEPP +KK K A N  SAS
Sbjct: 245 NMFIILVPNKEMIRKPQEPPTRKKKKTAENEASAS 277

BLAST of CSPI02G05270 vs. TAIR 10
Match: AT4G30690.2 (Translation initiation factor 3 protein )

HSP 1 Score: 235.3 bits (599), Expect = 6.2e-62
Identity = 131/214 (61.21%), Postives = 156/214 (72.90%), Query Frame = 0

Query: 90  GGAPRSSGPPD----SKRSNDEIGLDISAIKSINVRLIDAEQNMVGVVSKAQAIQMAEDA 149
           GG   S  P D     K S D+  LDISAI+S  VRLID +QNM+G+VSK +A++ AEDA
Sbjct: 62  GGGGGSRFPGDRRGRQKESEDDDSLDISAIRSATVRLIDDQQNMIGLVSKEEAVRRAEDA 121

Query: 150 ELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHDY 209
           ELDLV++SPDADPPVVR+MDY+                              YNIDQHDY
Sbjct: 122 ELDLVILSPDADPPVVRMMDYS------------------------------YNIDQHDY 181

Query: 210 SVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQEEVGELATEEAKNFRDRN 269
           SVR++AA+KFL+DGDKVKVIVN+KGRENEFRNIAIEL+RRFQ E+GEL TEE+KNFRDRN
Sbjct: 182 SVRMRAARKFLQDGDKVKVIVNMKGRENEFRNIAIELLRRFQTEIGELGTEESKNFRDRN 241

Query: 270 IFIVLVPNKAVLQKAQE-PPKKKDKPAVNGVSAS 299
           +FIVLVPNK V++K QE PPKKK KPA + VSA+
Sbjct: 242 LFIVLVPNKEVIRKVQEPPPKKKKKPADDKVSAA 245

BLAST of CSPI02G05270 vs. TAIR 10
Match: AT1G34360.1 (translation initiation factor 3 (IF-3) family protein )

HSP 1 Score: 57.8 bits (138), Expect = 1.8e-08
Identity = 60/191 (31.41%), Postives = 96/191 (50.26%), Query Frame = 0

Query: 100 DSKRSNDEIGLDISAIKSINVRLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPP 159
           D  R N++I  D        VRL+  E +   VVS  +A++ A++ + DLV V  DA PP
Sbjct: 82  DGPRLNEKITGDY-------VRLVSEEGHC--VVSLREALRRAKELQCDLVEVQRDAKPP 141

Query: 160 VVRIMDYNKYRYELQK--KKRGQQKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLK 219
           V +I+ Y+   Y+  K  K+R + K++ A R D+KE++    I+  D   +   A K ++
Sbjct: 142 VCKIVKYSLELYKKAKVGKERAKAKRAEAIRPDIKEIRFTPKIEAKDLKFKSDQALKLME 201

Query: 220 DGDKVKVI-VNLKGRENEFR-NIAIELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKA 279
            G +VK + V  K +  E      +EL+ RF   +G+ A  E+    DR   +V+V +  
Sbjct: 202 SGYRVKCLAVPDKDKHKELEPEKLLELLFRFTCFIGD-ALVESWPEADRKGAVVIVRHAK 257

Query: 280 VLQKAQEPPKK 287
                  PPKK
Sbjct: 262 F-----GPPKK 257

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94B528.7e-7771.96Translation initiation factor IF3-4, chloroplastic OS=Arabidopsis thaliana OX=37... [more]
O822344.5e-7359.27Translation initiation factor IF3-2, chloroplastic OS=Arabidopsis thaliana OX=37... [more]
B0JKQ15.5e-3140.91Translation initiation factor IF-3 OS=Microcystis aeruginosa (strain NIES-843) O... [more]
Q0IDZ52.7e-3045.14Translation initiation factor IF-3 OS=Synechococcus sp. (strain CC9311) OX=64471... [more]
A5GI063.6e-3043.50Translation initiation factor IF-3 OS=Synechococcus sp. (strain WH7803) OX=32051... [more]
Match NameE-valueIdentityDescription
A0A0A0LH346.5e-152100.00Translation initiation factor IF-3 OS=Cucumis sativus OX=3659 GN=Csa_2G036070 PE... [more]
A0A1S3BV824.9e-13993.33Translation initiation factor IF-3 OS=Cucumis melo OX=3656 GN=LOC103493630 PE=3 ... [more]
A0A6J1DYM51.9e-12282.94Translation initiation factor IF-3 OS=Momordica charantia OX=3673 GN=LOC11102471... [more]
A0A6J1G3Y59.9e-11679.67Translation initiation factor IF-3 OS=Cucurbita moschata OX=3662 GN=LOC111450575... [more]
A0A6J1KDY91.1e-11479.33Translation initiation factor IF-3 OS=Cucurbita maxima OX=3661 GN=LOC111493999 P... [more]
Match NameE-valueIdentityDescription
XP_004144726.11.4e-151100.00translation initiation factor IF3-4, chloroplastic [Cucumis sativus] >KGN61058.1... [more]
XP_008452687.11.0e-13893.33PREDICTED: translation initiation factor IF-3 [Cucumis melo][more]
XP_038891171.15.2e-12786.67translation initiation factor IF3-4, chloroplastic [Benincasa hispida][more]
XP_022158178.13.8e-12282.94translation initiation factor IF3-4, chloroplastic [Momordica charantia] >XP_022... [more]
XP_023547209.11.6e-11579.67translation initiation factor IF3-4, chloroplastic-like [Cucurbita pepo subsp. p... [more]
Match NameE-valueIdentityDescription
AT4G30690.16.2e-7871.96Translation initiation factor 3 protein [more]
AT2G24060.13.2e-7459.27Translation initiation factor 3 protein [more]
AT4G30690.26.2e-6261.21Translation initiation factor 3 protein [more]
AT1G34360.11.8e-0831.41translation initiation factor 3 (IF-3) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019815Translation initiation factor 3, C-terminalPFAMPF00707IF3_Ccoord: 190..273
e-value: 4.4E-23
score: 81.0
IPR019814Translation initiation factor 3, N-terminalPFAMPF05198IF3_Ncoord: 114..178
e-value: 2.3E-25
score: 88.6
IPR001288Translation initiation factor 3TIGRFAMTIGR00168TIGR00168coord: 114..274
e-value: 3.0E-47
score: 158.3
IPR001288Translation initiation factor 3PANTHERPTHR10938TRANSLATION INITIATION FACTOR IF-3coord: 52..290
IPR001288Translation initiation factor 3HAMAPMF_00080IF_3coord: 92..275
score: 22.629017
IPR036788Translation initiation factor 3 (IF-3), C-terminal domain superfamilyGENE3D3.30.110.10coord: 179..275
e-value: 1.8E-23
score: 84.4
IPR036788Translation initiation factor 3 (IF-3), C-terminal domain superfamilySUPERFAMILY55200Translation initiation factor IF3, C-terminal domaincoord: 189..274
IPR036787Translation initiation factor 3 (IF-3), N-terminal domain superfamilyGENE3D3.10.20.80coord: 113..178
e-value: 5.5E-27
score: 95.6
IPR036787Translation initiation factor 3 (IF-3), N-terminal domain superfamilySUPERFAMILY54364Translation initiation factor IF3, N-terminal domaincoord: 115..182
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 279..299
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 21..47
NoneNo IPR availablePANTHERPTHR10938:SF3TRANSLATION INITIATION FACTOR IF3-2, CHLOROPLASTICcoord: 52..290
IPR019813Translation initiation factor 3, conserved sitePROSITEPS00938IF3coord: 162..175

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G05270.1CSPI02G05270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032790 ribosome disassembly
biological_process GO:0006413 translational initiation
cellular_component GO:0009507 chloroplast
molecular_function GO:0043022 ribosome binding
molecular_function GO:0003743 translation initiation factor activity