CSPI02G04780 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G04780
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Pectinesterase
Location	Chr2: 3434664 .. 3436206 (-)
RNA-Seq Expression	CSPI02G04780
Synteny	CSPI02G04780

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGGTACAACCTCCATTTCTCTTGCTCTTCACTCTGTTCAATGCTGTTGTCTTTGGTTTTGAAGCTGTGAATCAGGCAAGAGACTTTGTCCTTACGGTTCAATCCATTGTTATTGTTGACAAGTCGGGAAATGGCAATTTCCAGACCGTTCAAGCAGCCATTGACTCAGTTCCACCTAATAACAATCATTGGATTAAGATTCAAATAAACCCTGGTGTATACAAGTAATACTAATACAGTACACATTATGAAATTTGGTATTGAGGTTTTGTTCTAATATTGCTGGCAAACCTTTTGCAGAGAAAAGGTGACAATCCCACTAGAAAAGCCTTTCATATATCTCGAAGGAGCTGACAGCAGTAATACGGTTATCACGTTCGATGACCATCAACAAACAGACACTAGTGCTACATTTACTTCACGTCCTCCCAACATTATTGTTAGAGGCATTACTTTTGAGGTGCTGTGGCTTCTAAAAACTGATTTTATATTCATAGCATTATTTGAGATTTTGAAGCTATGTAAGGTGCAAGTTAAGTTTGTAGTTAATGATATATATCGTACGGCGTACGCACCTGAGATAGGTTTGAGCAGGATTCTATAGCTCAATCATTGATCAATGTCTGTCTTGTTACAGAATTAATTGTTGCTTTTATATGGTAAATCAACATGCTTGATTATGTGTTATTGTGATGCACACAGAACTCATTCAACCTTCGAGAAGCTCCAGAGCTGTTTTCTTGCGATGATGGAACTTACATTACACAAGCAATAGCAGCAAGAATCTACGGCGACAAATCAGCTTTTTTCAACTGTGGATTCAAAGGGTATCAAGACACGTTATGGGATGTACAAGGTCGCCATTTCTTCTCACATTGCTACATTGAAGGAGCCATAGACTTCATCTTTGGTAGTGGCCAATCAGTTTATGAGGTACTCAGTTCAAATGTCAATATTCTGTCATTTACCCCATTGAAAGAGACTTACTTTTTAGCAACTTGGGACCATAAATGCATGTTTTTTTGACAATGTAGGATTGCATGATAAATGTTAATGTTGCAAGTCTTCCTCAAGTCTACCAAGGGTACATTACTGCACAAAGTCGACAATCAGCAGCAGATCCAAGTGGATTTGTGTTTAAAGAGTGCACCATAAAGGGTAGTGGCAAAGCTTTGTTGGGGAGAGCATATGGTCCTTTTTCCAGAGTTATATTCAAAGATGCTATAATGGGTTCAGTGGTAGCACCAGAGGGATGGTACGCCTGGCACTTCAAGGGCAAAGAGTAAGTTCAGGGCCAGAGTTCTTTCAAGCTGCACAGTGAAGCATAATAGTCATTTAGGACTTATTGAATTGCCAAGTTCTAACTGGTGTTACATTCTTTAGGGAAAATTTTATGTACGTTGAGGAGAATTGTACCGGACCAGGAGCTTCCACTTCCATGAGAGTTCCATGGGCAAAGACACTTGATGCCTCGCATCTAACAGGCTTCTCCGTGGAATCCTTCATTAACCAAGATGGGTGGATTCCCACAGTCCTTTGA

mRNA sequence

ATGCAGGTACAACCTCCATTTCTCTTGCTCTTCACTCTGTTCAATGCTGTTGTCTTTGGTTTTGAAGCTGTGAATCAGGCAAGAGACTTTGTCCTTACGGTTCAATCCATTGTTATTGTTGACAAGTCGGGAAATGGCAATTTCCAGACCGTTCAAGCAGCCATTGACTCAGTTCCACCTAATAACAATCATTGGATTAAGATTCAAATAAACCCTGGTGTATACAAAGAAAAGGTGACAATCCCACTAGAAAAGCCTTTCATATATCTCGAAGGAGCTGACAGCAGTAATACGGTTATCACGTTCGATGACCATCAACAAACAGACACTAGTGCTACATTTACTTCACGTCCTCCCAACATTATTGTTAGAGGCATTACTTTTGAGAACTCATTCAACCTTCGAGAAGCTCCAGAGCTGTTTTCTTGCGATGATGGAACTTACATTACACAAGCAATAGCAGCAAGAATCTACGGCGACAAATCAGCTTTTTTCAACTGTGGATTCAAAGGGTATCAAGACACGTTATGGGATGTACAAGGTCGCCATTTCTTCTCACATTGCTACATTGAAGGAGCCATAGACTTCATCTTTGGTAGTGGCCAATCAGTTTATGAGGATTGCATGATAAATGTTAATGTTGCAAGTCTTCCTCAAGTCTACCAAGGGTACATTACTGCACAAAGTCGACAATCAGCAGCAGATCCAAGTGGATTTGTGTTTAAAGAGTGCACCATAAAGGGTAGTGGCAAAGCTTTGTTGGGGAGAGCATATGGTCCTTTTTCCAGAGTTATATTCAAAGATGCTATAATGGGTTCAGTGGTAGCACCAGAGGGATGGTACGCCTGGCACTTCAAGGGCAAAGAGGAAAATTTTATGTACGTTGAGGAGAATTGTACCGGACCAGGAGCTTCCACTTCCATGAGAGTTCCATGGGCAAAGACACTTGATGCCTCGCATCTAACAGGCTTCTCCGTGGAATCCTTCATTAACCAAGATGGGTGGATTCCCACAGTCCTTTGA

Coding sequence (CDS)

ATGCAGGTACAACCTCCATTTCTCTTGCTCTTCACTCTGTTCAATGCTGTTGTCTTTGGTTTTGAAGCTGTGAATCAGGCAAGAGACTTTGTCCTTACGGTTCAATCCATTGTTATTGTTGACAAGTCGGGAAATGGCAATTTCCAGACCGTTCAAGCAGCCATTGACTCAGTTCCACCTAATAACAATCATTGGATTAAGATTCAAATAAACCCTGGTGTATACAAAGAAAAGGTGACAATCCCACTAGAAAAGCCTTTCATATATCTCGAAGGAGCTGACAGCAGTAATACGGTTATCACGTTCGATGACCATCAACAAACAGACACTAGTGCTACATTTACTTCACGTCCTCCCAACATTATTGTTAGAGGCATTACTTTTGAGAACTCATTCAACCTTCGAGAAGCTCCAGAGCTGTTTTCTTGCGATGATGGAACTTACATTACACAAGCAATAGCAGCAAGAATCTACGGCGACAAATCAGCTTTTTTCAACTGTGGATTCAAAGGGTATCAAGACACGTTATGGGATGTACAAGGTCGCCATTTCTTCTCACATTGCTACATTGAAGGAGCCATAGACTTCATCTTTGGTAGTGGCCAATCAGTTTATGAGGATTGCATGATAAATGTTAATGTTGCAAGTCTTCCTCAAGTCTACCAAGGGTACATTACTGCACAAAGTCGACAATCAGCAGCAGATCCAAGTGGATTTGTGTTTAAAGAGTGCACCATAAAGGGTAGTGGCAAAGCTTTGTTGGGGAGAGCATATGGTCCTTTTTCCAGAGTTATATTCAAAGATGCTATAATGGGTTCAGTGGTAGCACCAGAGGGATGGTACGCCTGGCACTTCAAGGGCAAAGAGGAAAATTTTATGTACGTTGAGGAGAATTGTACCGGACCAGGAGCTTCCACTTCCATGAGAGTTCCATGGGCAAAGACACTTGATGCCTCGCATCTAACAGGCTTCTCCGTGGAATCCTTCATTAACCAAGATGGGTGGATTCCCACAGTCCTTTGA

Protein sequence

MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL*

Homology

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BLAST of CSPI02G04780 vs. ExPASy Swiss-Prot
Match: Q4PSQ5 (Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2)

HSP 1 Score: 304.3 bits (778), Expect = 1.7e-81
Identity = 155/330 (46.97%), Postives = 211/330 (63.94%), Query Frame = 0


Query: 8   LLLFTLFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPPNNNHWIK 67
           +++ TLF   V+G+ A  +   + +T      VD +G GNF TVQ+AIDS+ P N++WI+
Sbjct: 14  MVVMTLF---VYGYSAAAEQIAYTIT------VDLNGGGNFTTVQSAIDSISPPNHNWIR 73

Query: 68  IQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPNIIVRGIT 127
           +    G+Y+EKVTIP EK FIYL+G     TVI +DDHQ TD SATFT+   +I++ GIT
Sbjct: 74  VFTQNGIYREKVTIPKEKGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGIT 133

Query: 128 FENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSH 187
           F+N++N+   P     ++   I  A+AAR+ GD+    +  F G QDTL+D +GRH++  
Sbjct: 134 FKNTYNI--VP-----NNKREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKR 193

Query: 188 CYIEGAIDFIFGSGQSVYEDCMINVNVA-SLPQVYQGYITAQSRQSAADPSGFVFKECTI 247
           C I G IDFIFG GQS++++C +N+ +    P    G ITA  R S +D  GFVF +CT+
Sbjct: 194 CIISGGIDFIFGYGQSLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTV 253

Query: 248 KGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGAST 307
            G GK LLGRA+G  +RVIF  + +  VV P GW AW  KG E +  +VE  CTG GA T
Sbjct: 254 TGVGKTLLGRAWGSNARVIFDRSRLSDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADT 313

Query: 308 SMRVPWAKTLDASHLTGFSVESFINQDGWI 337
           S RVPW K L  S + GF+  SFI+QDGWI
Sbjct: 314 SQRVPWLKKLSLSEVDGFASVSFIDQDGWI 327

[Close]

BLAST of CSPI02G04780 vs. ExPASy Swiss-Prot
Match: Q3E9D3 (Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 9.5e-80
Identity = 145/299 (48.49%), Postives = 192/299 (64.21%), Query Frame = 0


Query: 38  VIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSN 97
           VIVD+SG+GNF T+Q AIDSVP NN HW  I +  G+Y+EK+TIP +KPFI + GA   +
Sbjct: 29  VIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRS 88

Query: 98  TVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAIAARI 157
           T + +DDH     S TF +   N +V+ ITF NS+N     ++    +      A+AA I
Sbjct: 89  TRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKI----NKNPRVPAVAAFI 148

Query: 158 YGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASL 217
            GDKSAF++ GF G QDTLWD  GRH+F  C I+GA+DFI GSGQS+Y+ C+I V    L
Sbjct: 149 GGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQL 208

Query: 218 PQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAP 277
                GYITAQ R +A D +GFVF  C + G GKA LGRA+ P+SRVIF ++ +  VV P
Sbjct: 209 GPGVTGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDP 268

Query: 278 EGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWI 337
            GW+ W+++G E+   Y E  C G G++TS R  W K L AS +   +  SFIN+ GW+
Sbjct: 269 LGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWV 323

[Close]

BLAST of CSPI02G04780 vs. ExPASy Swiss-Prot
Match: Q4PSN0 (Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.1e-78
Identity = 143/302 (47.35%), Postives = 188/302 (62.25%), Query Frame = 0


Query: 35  QSIVIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGAD 94
           Q  V VD+SG+GNF T+Q AIDSVP NN HW  I +  G+Y+EK+ IP EKPFI L GA 
Sbjct: 31  QQQVFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAG 90

Query: 95  SSNTVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAIA 154
              T + +DDH     S TF++   N +V+ ITF NS+N     ++    +    T A+A
Sbjct: 91  KRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKM----NKNPRTPAVA 150

Query: 155 ARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNV 214
           A I GDKSAF++ GF G QDTLWD  GRH+F  C I+GA+DFIFG+GQS+Y+ C+I V  
Sbjct: 151 ALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLG 210

Query: 215 ASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSV 274
             L     GYITAQ R +  D +GF+F  C + G+G A LGR +  +SRVIF ++ +  V
Sbjct: 211 GQLEPGLAGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDV 270

Query: 275 VAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDG 334
           V PEGW AW+F G E   ++ E  C G GA+   RV W K L  S +   +  SFIN+ G
Sbjct: 271 VVPEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGG 328

Query: 335 WI 337
           W+
Sbjct: 331 WV 328

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BLAST of CSPI02G04780 vs. ExPASy Swiss-Prot
Match: O64479 (Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 6.4e-76
Identity = 141/305 (46.23%), Postives = 191/305 (62.62%), Query Frame = 0


Query: 34  VQSIVIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGA 93
           +   +IV+ +    F+TVQ+AIDS+P  N  WI+I I+ G+Y EKVTIP  K +IY++G 
Sbjct: 37  IAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQGG 96

Query: 94  DSSNTVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAI 153
               T+I + DHQ T+TSATFTS P NII+ GITF+N +N+            +    A+
Sbjct: 97  GIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNI--------ASSSSPTKPAV 156

Query: 154 AARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVN 213
           AA + GDK A  +  F G+QDTL+D  GRH++  C I G IDFIFG  QS++E C + + 
Sbjct: 157 AAMMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLR 216

Query: 214 VASLP--QVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIM 273
           V   P  +VY G ITAQ R S  D  GFVFK+CT+ GSGKALLGRA+  +SRVIF  ++ 
Sbjct: 217 VGIYPPNEVY-GTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMF 276

Query: 274 GSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFIN 333
              + P GW AW  KG+E +  +VE  CTG GA TS RVPW        +  F+  +FI+
Sbjct: 277 SDNILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFID 332

Query: 334 QDGWI 337
           ++GW+
Sbjct: 337 EEGWL 332

[Close]

BLAST of CSPI02G04780 vs. ExPASy Swiss-Prot
Match: O04953 (Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2)

HSP 1 Score: 258.1 bits (658), Expect = 1.4e-67
Identity = 128/245 (52.24%), Postives = 161/245 (65.71%), Query Frame = 0


Query: 98  TVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTY-ITQAIAAR 157
           T IT++ H  TD S+TFTS P +I+VR ++  N++N      L   +  ++ I  A+A  
Sbjct: 48  TTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYN--RLTSLTKANGMSWDIKPAVAIS 107

Query: 158 IYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVAS 217
           +YGDKSAF+NC F G QDT+WD  GRH F +CYIEGAIDFIFGSGQSVYEDC IN    +
Sbjct: 108 VYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINATAGA 167

Query: 218 L-PQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVV 277
           L  +V  GYITAQ R S +DPSGFVF   ++ GS    LGRAYGPFSRVIF    + SVV
Sbjct: 168 LASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDLSSVV 227

Query: 278 APEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHL-TGFSVESFINQDG 337
            PEGWY+WH+ G E +F Y E  C G G+  S RVPW   L + +    FS+ +FI+QD 
Sbjct: 228 HPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFIDQDQ 287

Query: 338 WIPTV 340
           WI  +
Sbjct: 288 WISNI 290

[Close]

BLAST of CSPI02G04780 vs. ExPASy TrEMBL
Match: A0A0A0LH00 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G034610 PE=4 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.3e-199
Identity = 338/340 (99.41%), Postives = 340/340 (100.00%), Query Frame = 0


Query: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPP 60
           MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLT+QS+VIVDKSGNGNFQTVQAAIDSVPP
Sbjct: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120
           NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN
Sbjct: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120

Query: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180
           IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ
Sbjct: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180

Query: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240
           GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV
Sbjct: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240

Query: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300
           FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT
Sbjct: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300

Query: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL 341
           GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL
Sbjct: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL 340

[Close]

BLAST of CSPI02G04780 vs. ExPASy TrEMBL
Match: A0A5A7U1G9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G001480 PE=4 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 2.3e-177
Identity = 303/337 (89.91%), Postives = 316/337 (93.77%), Query Frame = 0


Query: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPP 60
           MQ+QPPFLLLFTLFNAVVFGFEAVN AR F LTVQS+V+VDKSG G+FQTVQAAIDSVPP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120
           NNN WIKIQINPGVYKEKVTIP EKPFIYLEGAD SNT+ITFDDHQQTDTSATFTSRPPN
Sbjct: 61  NNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPN 120

Query: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180
           II RGITFENSFNL EAPELF CDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ
Sbjct: 121 IIARGITFENSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180

Query: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240
           GRHFF +CYIEGAIDFIFGSGQSVY+DC+INVNVASLPQV+QG+ITAQ R+SAADPSGFV
Sbjct: 181 GRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFV 240

Query: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300
           FK CTI GSGKALLGRAYGPFSRVIFKDAIMG VVAPEGW AW+FK KE+NFMYVEENCT
Sbjct: 241 FKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCT 300

Query: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIP 338
           GPGASTS RVPWAKTLDAS L  FSVESFINQDGWIP
Sbjct: 301 GPGASTSTRVPWAKTLDASQLRRFSVESFINQDGWIP 337

[Close]

BLAST of CSPI02G04780 vs. ExPASy TrEMBL
Match: A0A5D3BBR9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001020 PE=4 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 8.7e-177
Identity = 302/337 (89.61%), Postives = 315/337 (93.47%), Query Frame = 0


Query: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPP 60
           MQ+QPPFLLLFTLFNAVVFGFEAVN AR F LTVQS+V+VDKSG G+FQTVQAAIDSVPP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120
           NNN WIKIQINPGVYKEKVTIP EKPFIYLEGAD SNT+ITFDDHQQTDTSATFTSRPPN
Sbjct: 61  NNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPN 120

Query: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180
           II RGITFENSFNL EAPELF CDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ
Sbjct: 121 IIARGITFENSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180

Query: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240
           GRHFF +CYIEGAIDFIFGSGQSVY+DC+INVNVASLPQV+QG+ITAQ R+SAADPSGFV
Sbjct: 181 GRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFV 240

Query: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300
           FK C I GSGKALLGRAYGPFSRVIFKDAIMG VVAPEGW AW+FK KE+NFMYVEENCT
Sbjct: 241 FKGCAITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCT 300

Query: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIP 338
           GPGASTS RVPWAKTLDAS L  FSVESFINQDGWIP
Sbjct: 301 GPGASTSTRVPWAKTLDASQLRRFSVESFINQDGWIP 337

[Close]

BLAST of CSPI02G04780 vs. ExPASy TrEMBL
Match: A0A1S4DZS3 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499379 PE=4 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.1e-171
Identity = 303/367 (82.56%), Postives = 317/367 (86.38%), Query Frame = 0


Query: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPP 60
           MQ+QPPFLLLFTLFNAVVFGFEAVN AR F LTVQS+V+VDKSG G+FQTVQAAIDSVPP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVY------KEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATF 120
           NNN WIKIQINPGVY      +EKVTIP EKPFIYLEGAD SNT+ITFDDHQQTDTSATF
Sbjct: 61  NNNQWIKIQINPGVYNWQTFCREKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATF 120

Query: 121 TSRPPNIIVRGITFE------------------------NSFNLREAPELFSCDDGTYIT 180
           TSRPPNII RGITFE                        NSFNL EAPELF CDDGTYIT
Sbjct: 121 TSRPPNIIARGITFEVLWLLKTEFIFIASFEILKPKCTQNSFNLEEAPELFVCDDGTYIT 180

Query: 181 QAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMI 240
           QAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFF +CYIEGAIDFIFGSGQSVY+DC+I
Sbjct: 181 QAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLI 240

Query: 241 NVNVASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAI 300
           NVNVASLPQV+QG+ITAQ R+SAADPSGFVFK CTI GSGKALLGRAYGPFSRVIFKDAI
Sbjct: 241 NVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAI 300

Query: 301 MGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFI 338
           MG VVAPEGW AW+FK KE+NFMYVEENCTGPGASTSMRVPWAKTLDAS L  FSVESFI
Sbjct: 301 MGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESFI 360

[Close]

BLAST of CSPI02G04780 vs. ExPASy TrEMBL
Match: A0A6J1G5A0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111450826 PE=4 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.7e-130
Identity = 226/328 (68.90%), Postives = 260/328 (79.27%), Query Frame = 0


Query: 13  LFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINP 72
           +FNA      A+N  R   L +QS+++VDKSG GNF+T+QAAIDSVP NNN W+KIQ+NP
Sbjct: 1   MFNA------AMNFTRALDLKIQSVIVVDKSGKGNFKTIQAAIDSVPLNNNKWVKIQVNP 60

Query: 73  GVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPNIIVRGITFENSF 132
           G Y+E VTIP  K FIYL+G   S TVITF+ HQQT+TSATF S   NII RGITFENSF
Sbjct: 61  GEYREMVTIPSTKSFIYLQGGSRSTTVITFNGHQQTNTSATFNSMADNIIARGITFENSF 120

Query: 133 NLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEG 192
           NL  AP+LF    GT+ TQA+AARIYGDKSAF +CGFKG+QDTLWDV+GRH+F  CYIEG
Sbjct: 121 NLANAPQLFDKITGTFFTQALAARIYGDKSAFISCGFKGFQDTLWDVKGRHYFKSCYIEG 180

Query: 193 AIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKA 252
           AIDFIFG+GQS+YE C+I VNV  LP+V QGYITAQ RQSA DPSGFVF++CTI GSGK 
Sbjct: 181 AIDFIFGNGQSIYEGCLIYVNVGLLPEVNQGYITAQGRQSANDPSGFVFQQCTITGSGKG 240

Query: 253 LLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPW 312
            LGRAYGPFSRVIFKD  MG VVAPEGW AWHFKG+E NF+Y+E N  G G+S S RVPW
Sbjct: 241 FLGRAYGPFSRVIFKDTNMGKVVAPEGWDAWHFKGQETNFVYIESNSRGIGSSPSKRVPW 300

Query: 313 AKTLDASHLTGFSVESFINQDGWIPTVL 341
           A T   S L+G+SV+SFINQDGWIP  L
Sbjct: 301 AMTQGNSQLSGYSVKSFINQDGWIPNFL 322

[Close]

BLAST of CSPI02G04780 vs. NCBI nr
Match: KGN61008.1 (hypothetical protein Csa_021278 [Cucumis sativus])

HSP 1 Score: 705.3 bits (1819), Expect = 2.6e-199
Identity = 338/340 (99.41%), Postives = 340/340 (100.00%), Query Frame = 0


Query: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPP 60
           MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLT+QS+VIVDKSGNGNFQTVQAAIDSVPP
Sbjct: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120
           NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN
Sbjct: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120

Query: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180
           IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ
Sbjct: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180

Query: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240
           GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV
Sbjct: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240

Query: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300
           FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT
Sbjct: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300

Query: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL 341
           GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL
Sbjct: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL 340

[Close]

BLAST of CSPI02G04780 vs. NCBI nr
Match: XP_031736683.1 (probable pectinesterase 66 [Cucumis sativus])

HSP 1 Score: 692.6 bits (1786), Expect = 1.7e-195
Identity = 338/362 (93.37%), Postives = 340/362 (93.92%), Query Frame = 0


Query: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPP 60
           MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLT+QS+VIVDKSGNGNFQTVQAAIDSVPP
Sbjct: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120
           NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN
Sbjct: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120

Query: 121 IIVRGITFE----------------------NSFNLREAPELFSCDDGTYITQAIAARIY 180
           IIVRGITFE                      NSFNLREAPELFSCDDGTYITQAIAARIY
Sbjct: 121 IIVRGITFEVLWLLKTDFIFIALFEILKLCKNSFNLREAPELFSCDDGTYITQAIAARIY 180

Query: 181 GDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLP 240
           GDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLP
Sbjct: 181 GDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLP 240

Query: 241 QVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPE 300
           QVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPE
Sbjct: 241 QVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPE 300

Query: 301 GWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPT 341
           GWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPT
Sbjct: 301 GWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPT 360

[Close]

BLAST of CSPI02G04780 vs. NCBI nr
Match: KAA0048077.1 (putative pectinesterase 66 [Cucumis melo var. makuwa])

HSP 1 Score: 631.3 bits (1627), Expect = 4.7e-177
Identity = 303/337 (89.91%), Postives = 316/337 (93.77%), Query Frame = 0


Query: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPP 60
           MQ+QPPFLLLFTLFNAVVFGFEAVN AR F LTVQS+V+VDKSG G+FQTVQAAIDSVPP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120
           NNN WIKIQINPGVYKEKVTIP EKPFIYLEGAD SNT+ITFDDHQQTDTSATFTSRPPN
Sbjct: 61  NNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPN 120

Query: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180
           II RGITFENSFNL EAPELF CDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ
Sbjct: 121 IIARGITFENSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180

Query: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240
           GRHFF +CYIEGAIDFIFGSGQSVY+DC+INVNVASLPQV+QG+ITAQ R+SAADPSGFV
Sbjct: 181 GRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFV 240

Query: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300
           FK CTI GSGKALLGRAYGPFSRVIFKDAIMG VVAPEGW AW+FK KE+NFMYVEENCT
Sbjct: 241 FKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCT 300

Query: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIP 338
           GPGASTS RVPWAKTLDAS L  FSVESFINQDGWIP
Sbjct: 301 GPGASTSTRVPWAKTLDASQLRRFSVESFINQDGWIP 337

[Close]

BLAST of CSPI02G04780 vs. NCBI nr
Match: TYJ96459.1 (putative pectinesterase 66 [Cucumis melo var. makuwa])

HSP 1 Score: 629.4 bits (1622), Expect = 1.8e-176
Identity = 302/337 (89.61%), Postives = 315/337 (93.47%), Query Frame = 0


Query: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPP 60
           MQ+QPPFLLLFTLFNAVVFGFEAVN AR F LTVQS+V+VDKSG G+FQTVQAAIDSVPP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120
           NNN WIKIQINPGVYKEKVTIP EKPFIYLEGAD SNT+ITFDDHQQTDTSATFTSRPPN
Sbjct: 61  NNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPN 120

Query: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180
           II RGITFENSFNL EAPELF CDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ
Sbjct: 121 IIARGITFENSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180

Query: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240
           GRHFF +CYIEGAIDFIFGSGQSVY+DC+INVNVASLPQV+QG+ITAQ R+SAADPSGFV
Sbjct: 181 GRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFV 240

Query: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300
           FK C I GSGKALLGRAYGPFSRVIFKDAIMG VVAPEGW AW+FK KE+NFMYVEENCT
Sbjct: 241 FKGCAITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCT 300

Query: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIP 338
           GPGASTS RVPWAKTLDAS L  FSVESFINQDGWIP
Sbjct: 301 GPGASTSTRVPWAKTLDASQLRRFSVESFINQDGWIP 337

[Close]

BLAST of CSPI02G04780 vs. NCBI nr
Match: XP_016901459.1 (PREDICTED: probable pectinesterase 66 [Cucumis melo])

HSP 1 Score: 612.5 bits (1578), Expect = 2.3e-171
Identity = 303/367 (82.56%), Postives = 317/367 (86.38%), Query Frame = 0


Query: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPP 60
           MQ+QPPFLLLFTLFNAVVFGFEAVN AR F LTVQS+V+VDKSG G+FQTVQAAIDSVPP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVY------KEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATF 120
           NNN WIKIQINPGVY      +EKVTIP EKPFIYLEGAD SNT+ITFDDHQQTDTSATF
Sbjct: 61  NNNQWIKIQINPGVYNWQTFCREKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATF 120

Query: 121 TSRPPNIIVRGITFE------------------------NSFNLREAPELFSCDDGTYIT 180
           TSRPPNII RGITFE                        NSFNL EAPELF CDDGTYIT
Sbjct: 121 TSRPPNIIARGITFEVLWLLKTEFIFIASFEILKPKCTQNSFNLEEAPELFVCDDGTYIT 180

Query: 181 QAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMI 240
           QAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFF +CYIEGAIDFIFGSGQSVY+DC+I
Sbjct: 181 QAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLI 240

Query: 241 NVNVASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAI 300
           NVNVASLPQV+QG+ITAQ R+SAADPSGFVFK CTI GSGKALLGRAYGPFSRVIFKDAI
Sbjct: 241 NVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAI 300

Query: 301 MGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFI 338
           MG VVAPEGW AW+FK KE+NFMYVEENCTGPGASTSMRVPWAKTLDAS L  FSVESFI
Sbjct: 301 MGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESFI 360

[Close]

BLAST of CSPI02G04780 vs. TAIR 10
Match: AT2G47280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 304.3 bits (778), Expect = 1.2e-82
Identity = 155/330 (46.97%), Postives = 211/330 (63.94%), Query Frame = 0


Query: 8   LLLFTLFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPPNNNHWIK 67
           +++ TLF   V+G+ A  +   + +T      VD +G GNF TVQ+AIDS+ P N++WI+
Sbjct: 14  MVVMTLF---VYGYSAAAEQIAYTIT------VDLNGGGNFTTVQSAIDSISPPNHNWIR 73

Query: 68  IQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPNIIVRGIT 127
           +    G+Y+EKVTIP EK FIYL+G     TVI +DDHQ TD SATFT+   +I++ GIT
Sbjct: 74  VFTQNGIYREKVTIPKEKGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGIT 133

Query: 128 FENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSH 187
           F+N++N+   P     ++   I  A+AAR+ GD+    +  F G QDTL+D +GRH++  
Sbjct: 134 FKNTYNI--VP-----NNKREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKR 193

Query: 188 CYIEGAIDFIFGSGQSVYEDCMINVNVA-SLPQVYQGYITAQSRQSAADPSGFVFKECTI 247
           C I G IDFIFG GQS++++C +N+ +    P    G ITA  R S +D  GFVF +CT+
Sbjct: 194 CIISGGIDFIFGYGQSLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTV 253

Query: 248 KGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGAST 307
            G GK LLGRA+G  +RVIF  + +  VV P GW AW  KG E +  +VE  CTG GA T
Sbjct: 254 TGVGKTLLGRAWGSNARVIFDRSRLSDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADT 313

Query: 308 SMRVPWAKTLDASHLTGFSVESFINQDGWI 337
           S RVPW K L  S + GF+  SFI+QDGWI
Sbjct: 314 SQRVPWLKKLSLSEVDGFASVSFIDQDGWI 327

[Close]

BLAST of CSPI02G04780 vs. TAIR 10
Match: AT5G18990.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 298.5 bits (763), Expect = 6.8e-81
Identity = 145/299 (48.49%), Postives = 192/299 (64.21%), Query Frame = 0


Query: 38  VIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSN 97
           VIVD+SG+GNF T+Q AIDSVP NN HW  I +  G+Y+EK+TIP +KPFI + GA   +
Sbjct: 29  VIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRS 88

Query: 98  TVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAIAARI 157
           T + +DDH     S TF +   N +V+ ITF NS+N     ++    +      A+AA I
Sbjct: 89  TRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKI----NKNPRVPAVAAFI 148

Query: 158 YGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASL 217
            GDKSAF++ GF G QDTLWD  GRH+F  C I+GA+DFI GSGQS+Y+ C+I V    L
Sbjct: 149 GGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQL 208

Query: 218 PQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAP 277
                GYITAQ R +A D +GFVF  C + G GKA LGRA+ P+SRVIF ++ +  VV P
Sbjct: 209 GPGVTGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDP 268

Query: 278 EGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWI 337
            GW+ W+++G E+   Y E  C G G++TS R  W K L AS +   +  SFIN+ GW+
Sbjct: 269 LGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWV 323

[Close]

BLAST of CSPI02G04780 vs. TAIR 10
Match: AT3G24130.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 295.0 bits (754), Expect = 7.5e-80
Identity = 143/302 (47.35%), Postives = 188/302 (62.25%), Query Frame = 0


Query: 35  QSIVIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGAD 94
           Q  V VD+SG+GNF T+Q AIDSVP NN HW  I +  G+Y+EK+ IP EKPFI L GA 
Sbjct: 31  QQQVFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAG 90

Query: 95  SSNTVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAIA 154
              T + +DDH     S TF++   N +V+ ITF NS+N     ++    +    T A+A
Sbjct: 91  KRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKM----NKNPRTPAVA 150

Query: 155 ARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNV 214
           A I GDKSAF++ GF G QDTLWD  GRH+F  C I+GA+DFIFG+GQS+Y+ C+I V  
Sbjct: 151 ALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLG 210

Query: 215 ASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSV 274
             L     GYITAQ R +  D +GF+F  C + G+G A LGR +  +SRVIF ++ +  V
Sbjct: 211 GQLEPGLAGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDV 270

Query: 275 VAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDG 334
           V PEGW AW+F G E   ++ E  C G GA+   RV W K L  S +   +  SFIN+ G
Sbjct: 271 VVPEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGG 328

Query: 335 WI 337
           W+
Sbjct: 331 WV 328

[Close]

BLAST of CSPI02G04780 vs. TAIR 10
Match: AT2G19150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 285.8 bits (730), Expect = 4.6e-77
Identity = 141/305 (46.23%), Postives = 191/305 (62.62%), Query Frame = 0


Query: 34  VQSIVIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGA 93
           +   +IV+ +    F+TVQ+AIDS+P  N  WI+I I+ G+Y EKVTIP  K +IY++G 
Sbjct: 37  IAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQGG 96

Query: 94  DSSNTVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAI 153
               T+I + DHQ T+TSATFTS P NII+ GITF+N +N+            +    A+
Sbjct: 97  GIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNI--------ASSSSPTKPAV 156

Query: 154 AARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVN 213
           AA + GDK A  +  F G+QDTL+D  GRH++  C I G IDFIFG  QS++E C + + 
Sbjct: 157 AAMMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLR 216

Query: 214 VASLP--QVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIM 273
           V   P  +VY G ITAQ R S  D  GFVFK+CT+ GSGKALLGRA+  +SRVIF  ++ 
Sbjct: 217 VGIYPPNEVY-GTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMF 276

Query: 274 GSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFIN 333
              + P GW AW  KG+E +  +VE  CTG GA TS RVPW        +  F+  +FI+
Sbjct: 277 SDNILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFID 332

Query: 334 QDGWI 337
           ++GW+
Sbjct: 337 EEGWL 332

[Close]

BLAST of CSPI02G04780 vs. TAIR 10
Match: AT5G26810.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 258.1 bits (658), Expect = 1.0e-68
Identity = 128/245 (52.24%), Postives = 161/245 (65.71%), Query Frame = 0


Query: 98  TVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTY-ITQAIAAR 157
           T IT++ H  TD S+TFTS P +I+VR ++  N++N      L   +  ++ I  A+A  
Sbjct: 48  TTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYN--RLTSLTKANGMSWDIKPAVAIS 107

Query: 158 IYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVAS 217
           +YGDKSAF+NC F G QDT+WD  GRH F +CYIEGAIDFIFGSGQSVYEDC IN    +
Sbjct: 108 VYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINATAGA 167

Query: 218 L-PQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVV 277
           L  +V  GYITAQ R S +DPSGFVF   ++ GS    LGRAYGPFSRVIF    + SVV
Sbjct: 168 LASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDLSSVV 227

Query: 278 APEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHL-TGFSVESFINQDG 337
            PEGWY+WH+ G E +F Y E  C G G+  S RVPW   L + +    FS+ +FI+QD 
Sbjct: 228 HPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFIDQDQ 287

Query: 338 WIPTV 340
           WI  +
Sbjct: 288 WISNI 290

The following BLAST results are available for this feature:

BLAST of CSPI02G04780 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q4PSQ5	1.7e-81	46.97	Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2	[more]
Q3E9D3	9.5e-80	48.49	Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1	[more]
Q4PSN0	1.1e-78	47.35	Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1	[more]
O64479	6.4e-76	46.23	Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1	[more]
O04953	1.4e-67	52.24	Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2	[more]

BLAST of CSPI02G04780 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LH00	1.3e-199	99.41	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G034610 PE=4 SV=1	[more]
A0A5A7U1G9	2.3e-177	89.91	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00148...	[more]
A0A5D3BBR9	8.7e-177	89.61	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G00102...	[more]
A0A1S4DZS3	1.1e-171	82.56	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499379 PE=4 SV=1	[more]
A0A6J1G5A0	2.7e-130	68.90	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111450826 PE=4 SV=1	[more]

BLAST of CSPI02G04780 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN61008.1	2.6e-199	99.41	hypothetical protein Csa_021278 [Cucumis sativus]	[more]
XP_031736683.1	1.7e-195	93.37	probable pectinesterase 66 [Cucumis sativus]	[more]
KAA0048077.1	4.7e-177	89.91	putative pectinesterase 66 [Cucumis melo var. makuwa]	[more]
TYJ96459.1	1.8e-176	89.61	putative pectinesterase 66 [Cucumis melo var. makuwa]	[more]
XP_016901459.1	2.3e-171	82.56	PREDICTED: probable pectinesterase 66 [Cucumis melo]	[more]

BLAST of CSPI02G04780 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G47280.1	1.2e-82	46.97	Pectin lyase-like superfamily protein	[more]
AT5G18990.1	6.8e-81	48.49	Pectin lyase-like superfamily protein	[more]
AT3G24130.1	7.5e-80	47.35	Pectin lyase-like superfamily protein	[more]
AT2G19150.1	4.6e-77	46.23	Pectin lyase-like superfamily protein	[more]
AT5G26810.1	1.0e-68	52.24	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 38..332 e-value: 4.8E-61 score: 206.1
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 31..339 e-value: 3.1E-102 score: 344.2
None	No IPR available	PANTHER	PTHR31321:SF82	PECTINESTERASE	coord: 14..337
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 14..337
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 190..199
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 33..338

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G04780.1	CSPI02G04780.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity