CSPI02G04650 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G04650
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	urease accessory protein F
Location	Chr2: 3333233 .. 3335231 (-)
RNA-Seq Expression	CSPI02G04650
Synteny	CSPI02G04650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACATCATATGGAAATGGCGGATTTCAATTTTCATTCCCTTTAAGGTTAATTTTCATTCTTCCCATTTTTGTTCGTTTTGTTCTTCTTTGTGAAACAAATCTGTCATATGCTCTAGGGTTCGTATCTTGATTCTTGGCTGTTAATGAGGTTTTTAGTGGTTGCCCATTACAATGTTGATCGTCTTCGTCTTGGTTTAAGAAACAATTCAACGATTCTTGAGTTTTGAAATCTTGACTTGATCAGGGTTTGAGTTGGTTTTAATCAAACAGGAAATACATGGTTGGTTAGGTTAGTTTTGGTTACTCTGGAGTTCAGTCTCAAAACCTTGTTGGATTCTCTGTTTCTCTTTGGACTTCAGTTTTAAGGGCTTTTGAAGTTATTCGTTAGGTTTCATTTCACTTGATGATTGGACATCCGTCTTGTAGTTGACTTTCTCCGTTGTTCTTTGTGCTTCGGTTTTCTGGCGTTCTGAAATTATTCGTTAGTTTTTTTTTTTACTTGATTGGACCTTTGTCTTGTTGTTAACTTCCTTTTTGTGGCTTTATCATTTTGTATGTTTGTGTATTCTTTCTTTTCTCTTTTCCGAATGAAAATCAAATTTCTCAAACACAAAAGAAAATATTGTGGATTCAGATTGTTTTGACATCGAGTAGAGATGTGTAATTTATTGATTCTTGATGCACCCTTTGTGTATATATGTAGCACTTCTTGGTAAACCCTTGGTATATGTATAGCATTTCTTGGTGAGCCTTTTGTGTATGTACAGCATTTCTTGGTGAGCCCTTTGTGTTAGCATTTCTTGGTGAGCCCTTTGTGTATGTATACTATCCAGAGGGCATATTCCCTGAAAAAAAAAATCTTGTTGACAGTGTAAACATGTTTAGTTGTTATCATTTGTTAGATAAATTTGAAGTTAGCTACTTAAAACTCGAGTTTGTTATATTCATTTTCTAGATAGGTAACTTTAATGCAGTTGTGTTTTCTTGTTTCTGTTTTGCAGAATCTATGCAATAGTACAAACTTGACATGGATGACAAACATTGTCACTGGAGCCAATGGCAACTGCTCGATTCTATCCTTCCAACTGGTGGCTTCGCTCATTCATTCGGCCTTGAGGCTGCAATACAAGCTCAAATCGTCTCAAGTCCAGATGATCTCAAAACTTTTGTGATCCATTTATTGGACAACACTGGAAGTTTGTTTCTTCCCTTTGTTCATTCAGCCACACAATCGCCTGATTTTGAAACCTGGAAGAAAAACGACATGTTATTGGATGCAATGTTAACTAATGAAGTTAGTCGAAAGGCGTCTGTTACGCAAGGGTCAGCACTCATGAGAGTTTCAGCAATAGTATTTTCCGAAATCCCATCTCTGAAAGCAATGAGGGAGAACTTGTATGGGACTGGGGCTGTTTCATTCCACCATGCTCCCATCTTTGGGCTAATCTGCGGTCTACTTGGATGGGATGGCACAATGTCACAAAGAGCATATCTGTTTATTACCTTGAGAGATGTTATTTCTGCTGCAACAAGATTGAATTTAGTAGGACCTTTGGGTGCAGCTGTGTTGCAACATCAGCTTGCATTTGTTGCTGAAGACATATTGAAGAGATGGATGAACCGTCCTGTTGAAGAAGCTTGCCAAACGGTTCCTTTGTTAGAGACAGTACAGGGGTGCCATTCGTGCCTCTTTTCTAAAATGTTTTGTTCTTGAAAAATTTTTGAAGTGAAACTGCATCTTTCTTTTGATTTTGGGTATAGGAGTCCCCTTCTTTCATGTTCTCTGCTTGAAAATAGATGTGGATATGAGATAATAGTGGCAACGTCGAAATTCACGTACTCACATATATGCATTTCATTTTGTATGGCACTCCATGCTTTTGTTCCATTGAAGACTTTGTTTGTCTTTGGCCATGATTCAATATCAAGTGAATGTAAATAGACTTTTTAAGTACTTTGAAATCTTGATTATTCTGAACTTTTCAATTCTTTATCCGTCC

mRNA sequence

ACATCATATGGAAATGGCGGATTTCAATTTTCATTCCCTTTAAGAATCTATGCAATAGTACAAACTTGACATGGATGACAAACATTGTCACTGGAGCCAATGGCAACTGCTCGATTCTATCCTTCCAACTGGTGGCTTCGCTCATTCATTCGGCCTTGAGGCTGCAATACAAGCTCAAATCGTCTCAAGTCCAGATGATCTCAAAACTTTTGTGATCCATTTATTGGACAACACTGGAAGTTTGTTTCTTCCCTTTGTTCATTCAGCCACACAATCGCCTGATTTTGAAACCTGGAAGAAAAACGACATGTTATTGGATGCAATGTTAACTAATGAAGTTAGTCGAAAGGCGTCTGTTACGCAAGGGTCAGCACTCATGAGAGTTTCAGCAATAGTATTTTCCGAAATCCCATCTCTGAAAGCAATGAGGGAGAACTTGTATGGGACTGGGGCTGTTTCATTCCACCATGCTCCCATCTTTGGGCTAATCTGCGGTCTACTTGGATGGGATGGCACAATGTCACAAAGAGCATATCTGTTTATTACCTTGAGAGATGTTATTTCTGCTGCAACAAGATTGAATTTAGTAGGACCTTTGGGTGCAGCTGTGTTGCAACATCAGCTTGCATTTGTTGCTGAAGACATATTGAAGAGATGGATGAACCGTCCTGTTGAAGAAGCTTGCCAAACGGTTCCTTTGTTAGAGACAGTACAGGGGTGCCATTCGTGCCTCTTTTCTAAAATGTTTTGTTCTTGAAAAATTTTTGAAGTGAAACTGCATCTTTCTTTTGATTTTGGGTATAGGAGTCCCCTTCTTTCATGTTCTCTGCTTGAAAATAGATGTGGATATGAGATAATAGTGGCAACGTCGAAATTCACGTACTCACATATATGCATTTCATTTTGTATGGCACTCCATGCTTTTGTTCCATTGAAGACTTTGTTTGTCTTTGGCCATGATTCAATATCAAGTGAATGTAAATAGACTTTTTAAGTACTTTGAAATCTTGATTATTCTGAACTTTTCAATTCTTTATCCGTCC

Coding sequence (CDS)

ATGGATGACAAACATTGTCACTGGAGCCAATGGCAACTGCTCGATTCTATCCTTCCAACTGGTGGCTTCGCTCATTCATTCGGCCTTGAGGCTGCAATACAAGCTCAAATCGTCTCAAGTCCAGATGATCTCAAAACTTTTGTGATCCATTTATTGGACAACACTGGAAGTTTGTTTCTTCCCTTTGTTCATTCAGCCACACAATCGCCTGATTTTGAAACCTGGAAGAAAAACGACATGTTATTGGATGCAATGTTAACTAATGAAGTTAGTCGAAAGGCGTCTGTTACGCAAGGGTCAGCACTCATGAGAGTTTCAGCAATAGTATTTTCCGAAATCCCATCTCTGAAAGCAATGAGGGAGAACTTGTATGGGACTGGGGCTGTTTCATTCCACCATGCTCCCATCTTTGGGCTAATCTGCGGTCTACTTGGATGGGATGGCACAATGTCACAAAGAGCATATCTGTTTATTACCTTGAGAGATGTTATTTCTGCTGCAACAAGATTGAATTTAGTAGGACCTTTGGGTGCAGCTGTGTTGCAACATCAGCTTGCATTTGTTGCTGAAGACATATTGAAGAGATGGATGAACCGTCCTGTTGAAGAAGCTTGCCAAACGGTTCCTTTGTTAGAGACAGTACAGGGGTGCCATTCGTGCCTCTTTTCTAAAATGTTTTGTTCTTGA

Protein sequence

MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFLPFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMRENLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQHQLAFVAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS*

Homology

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BLAST of CSPI02G04650 vs. ExPASy Swiss-Prot
Match: Q9XHZ3 (Urease accessory protein F OS=Arabidopsis thaliana OX=3702 GN=UREF PE=2 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 3.8e-88
Identity = 151/221 (68.33%), Postives = 187/221 (84.62%), Query Frame = 0


Query: 8   WSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFLPFVHSAT 67
           WSQWQLLDSILPTGGFAHSFGLEAAIQ ++VSSP+DL+T +IH+LDNT SL LPFV+SA 
Sbjct: 20  WSQWQLLDSILPTGGFAHSFGLEAAIQTRLVSSPEDLETHIIHVLDNTASLLLPFVYSAL 79

Query: 68  QSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMRENLYGTG 127
           +SPD ETW K D +L+A LTN+VS KAS++QGSAL R++A VF+E+P+LK +R+   G+ 
Sbjct: 80  KSPDIETWHKLDGILNATLTNQVSSKASMSQGSALFRIAASVFTEVPNLKMIRDASLGSK 139

Query: 128 AVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQHQLAF 187
            V FHHAPIFGL+CGLLG D   SQRAYLF+TLRDV+SAATRLN+VGP+GA+V+QH++A 
Sbjct: 140 NVCFHHAPIFGLVCGLLGMDSETSQRAYLFVTLRDVLSAATRLNIVGPMGASVMQHRIAI 199

Query: 188 VAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 229
           V E +L++WMNR   EACQT PLL+ VQGCH  LFS++FCS
Sbjct: 200 VTETVLEKWMNREAGEACQTSPLLDVVQGCHGYLFSRLFCS 240

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BLAST of CSPI02G04650 vs. ExPASy Swiss-Prot
Match: E0ZS46 (Urease accessory protein F OS=Oryza sativa subsp. indica OX=39946 GN=UREF PE=2 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 7.3e-84
Identity = 145/226 (64.16%), Postives = 193/226 (85.40%), Query Frame = 0


Query: 3   DKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFLPF 62
           ++H  WSQWQLLDSILPTGGFAHS+GLEAA+Q+++V++P++L++FV+ +L+NTGSL LPF
Sbjct: 36  NQHSLWSQWQLLDSILPTGGFAHSYGLEAAMQSRMVNNPEELRSFVVQVLENTGSLLLPF 95

Query: 63  VHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMREN 122
           V  A +SPD  TW K D LL+AMLTNEVSRKAS++QGSAL+RV+A VF+EI SL+ +R+ 
Sbjct: 96  VCCANKSPDAATWVKLDQLLEAMLTNEVSRKASMSQGSALLRVAASVFTEIQSLQDLRQT 155

Query: 123 LYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQ 182
             G+  VSFHHAPIFGLICGL+G+D   +QRAY+F+T+RDVISAATRLNL+GPL A+VLQ
Sbjct: 156 FLGSKIVSFHHAPIFGLICGLVGFDSETTQRAYMFVTMRDVISAATRLNLIGPLAASVLQ 215

Query: 183 HQLAFVAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 229
           HQ+A  AE ++++W +R VEEA QT PLL+ +QGCH+ +FS++FC+
Sbjct: 216 HQVAEDAERMVQKWKDRGVEEATQTSPLLDALQGCHAYMFSRLFCT 261

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BLAST of CSPI02G04650 vs. ExPASy Swiss-Prot
Match: Q0E3L5 (Urease accessory protein F OS=Oryza sativa subsp. japonica OX=39947 GN=UREF PE=2 SV=2)

HSP 1 Score: 311.6 bits (797), Expect = 7.3e-84
Identity = 145/226 (64.16%), Postives = 193/226 (85.40%), Query Frame = 0


Query: 3   DKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFLPF 62
           ++H  WSQWQLLDSILPTGGFAHS+GLEAA+Q+++V++P++L++FV+ +L+NTGSL LPF
Sbjct: 36  NQHSLWSQWQLLDSILPTGGFAHSYGLEAAMQSRMVNNPEELRSFVVQVLENTGSLLLPF 95

Query: 63  VHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMREN 122
           V  A +SPD  TW K D LL+AMLTNEVSRKAS++QGSAL+RV+A VF+EI SL+ +R+ 
Sbjct: 96  VCCANKSPDAATWVKLDQLLEAMLTNEVSRKASMSQGSALLRVAASVFTEIQSLQDLRQT 155

Query: 123 LYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQ 182
             G+  VSFHHAPIFGLICGL+G+D   +QRAY+F+T+RDVISAATRLNL+GPL A+VLQ
Sbjct: 156 FLGSKIVSFHHAPIFGLICGLVGFDSETTQRAYMFVTMRDVISAATRLNLIGPLAASVLQ 215

Query: 183 HQLAFVAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 229
           HQ+A  AE ++++W +R VEEA QT PLL+ +QGCH+ +FS++FC+
Sbjct: 216 HQVAEDAERMVQKWKDRGVEEATQTSPLLDALQGCHAYMFSRLFCT 261

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BLAST of CSPI02G04650 vs. ExPASy Swiss-Prot
Match: B7K911 (Urease accessory protein UreF OS=Gloeothece citriformis (strain PCC 7424) OX=65393 GN=ureF PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.5e-12
Identity = 60/221 (27.15%), Postives = 101/221 (45.70%), Query Frame = 0


Query: 12  QLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDN----TGSLFLPFVHSAT 71
           QL DS  P+G F  S GLEA  Q + + S  DL+ F+  LL N    T  + L   +  +
Sbjct: 14  QLSDSFFPSGSFTLSHGLEALAQGEQIHSIKDLEIFLQILLHNKLGTTDLVALIHAYRGS 73

Query: 72  QSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMRENLYGTG 131
           +  D +  ++ D  L A    E +R+     G AL+ V+   + +       +E   GT 
Sbjct: 74  KREDLDAVREADHQLFAQTLIEKNREMGRKSGRALLMVARETWQDTQLETLEKETAKGT- 133

Query: 132 AVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQHQLAF 191
            ++  H  IF ++  + G   T +  A+L   +  ++ AA RLN++G L A  +  +LA 
Sbjct: 134 -INGLHPIIFAVVGRVAGLSETDTGLAFLHSFITGLLGAAIRLNIIGHLQAQKILLKLAP 193

Query: 192 VAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 229
             E   ++     + E     PL++  Q  H  L  ++F +
Sbjct: 194 DLETTYQKATEINLNEMFSCTPLIDIAQMNHQNLDYRLFAN 232

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BLAST of CSPI02G04650 vs. ExPASy Swiss-Prot
Match: Q117Z4 (Urease accessory protein UreF OS=Trichodesmium erythraeum (strain IMS101) OX=203124 GN=ureF PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 7.5e-12
Identity = 59/221 (26.70%), Postives = 102/221 (46.15%), Query Frame = 0


Query: 12  QLLDSILPTGGFAHSFGLEAAIQ-AQIVSSPDDLKTFVIHLLDNTG-SLFLPFVHS--AT 71
           QL DS  PTG F  S GLE  +Q  +I S P+ L    I L +  G S  +  +HS    
Sbjct: 14  QLSDSFFPTGSFTFSHGLETLVQTGKIQSQPEILYFLQILLRNKVGVSDVVALIHSYRGC 73

Query: 72  QSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMRENLYGTG 131
           ++ D E  +  D +L        +R+     G AL+ V++  + +   LK +  +   +G
Sbjct: 74  KNGDIEAVRVADRMLFVQTAIAKNRETQRQSGRALLMVASSTWQD-ERLKTLNIDAV-SG 133

Query: 132 AVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQHQLAF 191
            +   H  IFG +  ++G D   +  A+L   + +++ AA RL ++G + A  +  QL  
Sbjct: 134 NIHCLHPVIFGAVTSVVGLDERNAVLAFLHGLVTNILGAAIRLGILGHIQAQQILLQLVP 193

Query: 192 VAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 229
             E +    ++  +E+     P ++  Q  H  L  K+F +
Sbjct: 194 DIEAVWSTAVSMNLEQMWSCTPFIDIAQMQHPKLAHKLFAN 232

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BLAST of CSPI02G04650 vs. ExPASy TrEMBL
Match: A0A0A0LJ08 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G033980 PE=4 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 2.3e-128
Identity = 228/228 (100.00%), Postives = 228/228 (100.00%), Query Frame = 0


Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFL 60
           MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFL
Sbjct: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFL 60

Query: 61  PFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMR 120
           PFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMR
Sbjct: 61  PFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMR 120

Query: 121 ENLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           ENLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 121 ENLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180

Query: 181 LQHQLAFVAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 229
           LQHQLAFVAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS
Sbjct: 181 LQHQLAFVAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 228

[Close]

BLAST of CSPI02G04650 vs. ExPASy TrEMBL
Match: A0A5D3B9D0 (Lon protease-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001170 PE=3 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 3.3e-111
Identity = 202/214 (94.39%), Postives = 208/214 (97.20%), Query Frame = 0


Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFL 60
           MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSP+DLKTFVIHLLDNTGSLFL
Sbjct: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL 60

Query: 61  PFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMR 120
           PFV+SAT SPDFETWKKND LLDA+LTNEVSRKASVTQGSALMRVSAIVFSEI SLKAMR
Sbjct: 61  PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR 120

Query: 121 ENLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           E+LYGTG VSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 121 EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180

Query: 181 LQHQLAFVAEDILKRWMNRPVEEACQTVPLLETV 215
           LQHQLA VAE++LKRWMNRPVEEACQ VPLLETV
Sbjct: 181 LQHQLAQVAENVLKRWMNRPVEEACQAVPLLETV 214

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BLAST of CSPI02G04650 vs. ExPASy TrEMBL
Match: A0A6J1G3M3 (urease accessory protein F OS=Cucurbita moschata OX=3662 GN=LOC111450480 PE=4 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 3.3e-111
Identity = 201/228 (88.16%), Postives = 211/228 (92.54%), Query Frame = 0


Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFL 60
           MDDK  HWSQWQLLDSILPTGGFAHSFGLEAAIQA+IVS P+DLKTFVIHLLDNTGSL L
Sbjct: 8   MDDKQFHWSQWQLLDSILPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 67

Query: 61  PFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMR 120
           PFVHS T SPD ETW+KND  L+A+LTNEVSRKASVTQGSALMRV+AIVFSEI SLKAMR
Sbjct: 68  PFVHSTTLSPDLETWEKNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 127

Query: 121 ENLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           E   GTGAVSFHHAPIFGLICGLLGWD TMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 128 ETTTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 187

Query: 181 LQHQLAFVAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 229
           LQHQLA VAED LK+WMNRPVEEACQTVPLLETVQGCH+ LFS++FCS
Sbjct: 188 LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 235

[Close]

BLAST of CSPI02G04650 vs. ExPASy TrEMBL
Match: A0A5A7TYF8 (Lon protease-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G001330 PE=3 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 2.8e-110
Identity = 201/214 (93.93%), Postives = 207/214 (96.73%), Query Frame = 0


Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFL 60
           MDDK CHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSP+DLKTFVIHLLDNTGSLFL
Sbjct: 1   MDDKRCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL 60

Query: 61  PFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMR 120
           PFV+SAT SPDFETWKKND LLDA+LTNEVSRKASVTQGSALMRVSAIVFSEI SLKAMR
Sbjct: 61  PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR 120

Query: 121 ENLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           E+LYGTG VSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 121 EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180

Query: 181 LQHQLAFVAEDILKRWMNRPVEEACQTVPLLETV 215
           LQHQLA VAE++LKRWMNRPVEEACQ VPLLETV
Sbjct: 181 LQHQLAQVAENVLKRWMNRPVEEACQAVPLLETV 214

[Close]

BLAST of CSPI02G04650 vs. ExPASy TrEMBL
Match: A0A6J1KB78 (urease accessory protein F OS=Cucurbita maxima OX=3661 GN=LOC111493898 PE=4 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 3.6e-110
Identity = 199/228 (87.28%), Postives = 210/228 (92.11%), Query Frame = 0


Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFL 60
           MDDK  HWSQWQLLDSILPTGGFAHSFGLEAAIQA+IVS P+DLKTFVIHLLDNTGSL L
Sbjct: 8   MDDKQFHWSQWQLLDSILPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 67

Query: 61  PFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMR 120
           PFVHS T SPD ETW+KND  L+A+LTNEVSRKAS+TQGSALMRV+AIVFSEI SLKAMR
Sbjct: 68  PFVHSTTLSPDLETWEKNDRFLNAILTNEVSRKASITQGSALMRVAAIVFSEISSLKAMR 127

Query: 121 ENLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           E   GTGAVSFHHAPIFGLICGLLGWD TMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 128 ETSTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 187

Query: 181 LQHQLAFVAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 229
           LQHQLA VAE  LK+WMNRPVEEACQTVPLLETVQGCH+ LFS++FCS
Sbjct: 188 LQHQLALVAEGTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 235

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BLAST of CSPI02G04650 vs. NCBI nr
Match: XP_004144755.1 (urease accessory protein F [Cucumis sativus] >KGN60994.1 hypothetical protein Csa_021347 [Cucumis sativus])

HSP 1 Score: 468.0 bits (1203), Expect = 4.7e-128
Identity = 228/228 (100.00%), Postives = 228/228 (100.00%), Query Frame = 0


Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFL 60
           MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFL
Sbjct: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFL 60

Query: 61  PFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMR 120
           PFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMR
Sbjct: 61  PFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMR 120

Query: 121 ENLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           ENLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 121 ENLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180

Query: 181 LQHQLAFVAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 229
           LQHQLAFVAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS
Sbjct: 181 LQHQLAFVAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 228

[Close]

BLAST of CSPI02G04650 vs. NCBI nr
Match: XP_022946416.1 (urease accessory protein F [Cucurbita moschata])

HSP 1 Score: 411.0 bits (1055), Expect = 6.8e-111
Identity = 201/228 (88.16%), Postives = 211/228 (92.54%), Query Frame = 0


Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFL 60
           MDDK  HWSQWQLLDSILPTGGFAHSFGLEAAIQA+IVS P+DLKTFVIHLLDNTGSL L
Sbjct: 8   MDDKQFHWSQWQLLDSILPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 67

Query: 61  PFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMR 120
           PFVHS T SPD ETW+KND  L+A+LTNEVSRKASVTQGSALMRV+AIVFSEI SLKAMR
Sbjct: 68  PFVHSTTLSPDLETWEKNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 127

Query: 121 ENLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           E   GTGAVSFHHAPIFGLICGLLGWD TMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 128 ETTTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 187

Query: 181 LQHQLAFVAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 229
           LQHQLA VAED LK+WMNRPVEEACQTVPLLETVQGCH+ LFS++FCS
Sbjct: 188 LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 235

[Close]

BLAST of CSPI02G04650 vs. NCBI nr
Match: TYJ96472.1 (lon protease-like protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 411.0 bits (1055), Expect = 6.8e-111
Identity = 202/214 (94.39%), Postives = 208/214 (97.20%), Query Frame = 0


Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFL 60
           MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSP+DLKTFVIHLLDNTGSLFL
Sbjct: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL 60

Query: 61  PFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMR 120
           PFV+SAT SPDFETWKKND LLDA+LTNEVSRKASVTQGSALMRVSAIVFSEI SLKAMR
Sbjct: 61  PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR 120

Query: 121 ENLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           E+LYGTG VSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 121 EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180

Query: 181 LQHQLAFVAEDILKRWMNRPVEEACQTVPLLETV 215
           LQHQLA VAE++LKRWMNRPVEEACQ VPLLETV
Sbjct: 181 LQHQLAQVAENVLKRWMNRPVEEACQAVPLLETV 214

[Close]

BLAST of CSPI02G04650 vs. NCBI nr
Match: XP_038890480.1 (urease accessory protein F [Benincasa hispida])

HSP 1 Score: 411.0 bits (1055), Expect = 6.8e-111
Identity = 204/229 (89.08%), Postives = 213/229 (93.01%), Query Frame = 0


Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFL 60
           MDDKH HWSQWQLLDSILPTGGFAHSFGLEAAIQA+IVS  +DLK +VIHLLDNTGSL L
Sbjct: 1   MDDKHFHWSQWQLLDSILPTGGFAHSFGLEAAIQARIVSHQEDLKNYVIHLLDNTGSLLL 60

Query: 61  PFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMR 120
           PFVHSAT SPDFETWKKND LLD+MLTNEVSRKASVTQGSALMRV+AIVFSEI SLKAMR
Sbjct: 61  PFVHSATLSPDFETWKKNDRLLDSMLTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 120

Query: 121 E-NLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAA 180
           E  L GTGAVSFHHAPIFGLICGLLGWD TMSQRAYLFIT+RDVISAATRLNLVGPLGAA
Sbjct: 121 ETTLSGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITMRDVISAATRLNLVGPLGAA 180

Query: 181 VLQHQLAFVAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 229
           VLQHQLA VAEDILK+WMNRPVEEACQTVPLLETVQGCH  L S++FCS
Sbjct: 181 VLQHQLALVAEDILKKWMNRPVEEACQTVPLLETVQGCHMYLNSRLFCS 229

[Close]

BLAST of CSPI02G04650 vs. NCBI nr
Match: XP_023545319.1 (urease accessory protein F [Cucurbita pepo subsp. pepo])

HSP 1 Score: 410.6 bits (1054), Expect = 8.8e-111
Identity = 201/228 (88.16%), Postives = 211/228 (92.54%), Query Frame = 0


Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFL 60
           MDDK  HWSQWQLLDSILPTGGFAHSFGLEAAIQA+IVS P+DLKTFVIHLLDNTGSL L
Sbjct: 14  MDDKQFHWSQWQLLDSILPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 73

Query: 61  PFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMR 120
           PFVHS T SPD ETW+KND  L+A+LTNEVSRKASVTQGSALMRV+AIVFSEI SLKAMR
Sbjct: 74  PFVHSTTLSPDLETWEKNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 133

Query: 121 ENLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           E   GTGAVSFHHAPIFGLICGLLGWD TMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 134 ETSTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 193

Query: 181 LQHQLAFVAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 229
           LQHQLA VAED LK+WMNRPVEEACQTVPLLETVQGCH+ LFS++FCS
Sbjct: 194 LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 241

[Close]

BLAST of CSPI02G04650 vs. TAIR 10
Match: AT1G21840.1 (urease accessory protein F )

HSP 1 Score: 325.9 bits (834), Expect = 2.7e-89
Identity = 151/221 (68.33%), Postives = 187/221 (84.62%), Query Frame = 0


Query: 8   WSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFLPFVHSAT 67
           WSQWQLLDSILPTGGFAHSFGLEAAIQ ++VSSP+DL+T +IH+LDNT SL LPFV+SA 
Sbjct: 20  WSQWQLLDSILPTGGFAHSFGLEAAIQTRLVSSPEDLETHIIHVLDNTASLLLPFVYSAL 79

Query: 68  QSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMRENLYGTG 127
           +SPD ETW K D +L+A LTN+VS KAS++QGSAL R++A VF+E+P+LK +R+   G+ 
Sbjct: 80  KSPDIETWHKLDGILNATLTNQVSSKASMSQGSALFRIAASVFTEVPNLKMIRDASLGSK 139

Query: 128 AVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQHQLAF 187
            V FHHAPIFGL+CGLLG D   SQRAYLF+TLRDV+SAATRLN+VGP+GA+V+QH++A 
Sbjct: 140 NVCFHHAPIFGLVCGLLGMDSETSQRAYLFVTLRDVLSAATRLNIVGPMGASVMQHRIAI 199

Query: 188 VAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 229
           V E +L++WMNR   EACQT PLL+ VQGCH  LFS++FCS
Sbjct: 200 VTETVLEKWMNREAGEACQTSPLLDVVQGCHGYLFSRLFCS 240

The following BLAST results are available for this feature:

BLAST of CSPI02G04650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XHZ3	3.8e-88	68.33	Urease accessory protein F OS=Arabidopsis thaliana OX=3702 GN=UREF PE=2 SV=1	[more]
E0ZS46	7.3e-84	64.16	Urease accessory protein F OS=Oryza sativa subsp. indica OX=39946 GN=UREF PE=2 S...	[more]
Q0E3L5	7.3e-84	64.16	Urease accessory protein F OS=Oryza sativa subsp. japonica OX=39947 GN=UREF PE=2...	[more]
B7K911	1.5e-12	27.15	Urease accessory protein UreF OS=Gloeothece citriformis (strain PCC 7424) OX=653...	[more]
Q117Z4	7.5e-12	26.70	Urease accessory protein UreF OS=Trichodesmium erythraeum (strain IMS101) OX=203...	[more]

BLAST of CSPI02G04650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJ08	2.3e-128	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G033980 PE=4 SV=1	[more]
A0A5D3B9D0	3.3e-111	94.39	Lon protease-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A6J1G3M3	3.3e-111	88.16	urease accessory protein F OS=Cucurbita moschata OX=3662 GN=LOC111450480 PE=4 SV...	[more]
A0A5A7TYF8	2.8e-110	93.93	Lon protease-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]
A0A6J1KB78	3.6e-110	87.28	urease accessory protein F OS=Cucurbita maxima OX=3661 GN=LOC111493898 PE=4 SV=1	[more]

BLAST of CSPI02G04650 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144755.1	4.7e-128	100.00	urease accessory protein F [Cucumis sativus] >KGN60994.1 hypothetical protein Cs...	[more]
XP_022946416.1	6.8e-111	88.16	urease accessory protein F [Cucurbita moschata]	[more]
TYJ96472.1	6.8e-111	94.39	lon protease-like protein 1 [Cucumis melo var. makuwa]	[more]
XP_038890480.1	6.8e-111	89.08	urease accessory protein F [Benincasa hispida]	[more]
XP_023545319.1	8.8e-111	88.16	urease accessory protein F [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI02G04650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G21840.1	2.7e-89	68.33	urease accessory protein F	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002639	Urease accessory protein UreF	PFAM	PF01730	UreF	coord: 38..185 e-value: 3.2E-18 score: 66.6
IPR002639	Urease accessory protein UreF	PIRSF	PIRSF009467	Urease_acces_UreF	coord: 3..228 e-value: 1.6E-44 score: 150.4
IPR038277	UreF domain superfamily	GENE3D	1.10.4190.10	Urease accessory protein UreF	coord: 3..207 e-value: 4.4E-49 score: 169.3
None	No IPR available	PANTHER	PTHR33620	UREASE ACCESSORY PROTEIN F	coord: 1..228

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G04650.1	CSPI02G04650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030163	protein catabolic process
biological_process	GO:0006508	proteolysis
biological_process	GO:0006807	nitrogen compound metabolic process
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004176	ATP-dependent peptidase activity
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0016151	nickel cation binding
molecular_function	GO:0004252	serine-type endopeptidase activity