CSPI02G04380 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G04380
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionF-actin-capping protein subunit alpha
LocationChr2: 3151329 .. 3155737 (-)
RNA-Seq ExpressionCSPI02G04380
SyntenyCSPI02G04380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCTCCACCCTCTCTCCCTCCTTCAGTTTCAGTTCATTTGAAAAATCGGCGAAACGAATTTCTATTTTTCGGCAATGGCCGATGAAGAAGTGGAGCTCAGCGACGAGCAGAAGAAGGAGATCGCGAAGTGGTTTCTCCTAAACGCTCCAGCCGGAGAAATCCAATTCGTCGCCAAAGGTCGTCAATAGTTACATTTCTGTTCACGTAGATGTGTAATTTTGTATATGCATTGTGTACGGTCATTTCGGTGATTGTTGTTGTTCGAATTGTGTATTTGATGTTTCGTGTTTATTAATTTTCAGATGTGAAGAAAATTTTGAACGACGATGAGTTGTACCACGAGGCAGCATCTGAAGCTTTTCCTCAGTACAATAAATCACATATGATTTGTATTGAAATGCCTGGTCGAGTAGGAGATGTAAGTTTCTTATACGGATAATGTTTTTGTGCTGCTCTTTTCATTGCGGATAAGGTTGGGAATTAGTTTTTGTGGATTCGAGATAATTGTAGTGAGTGGCGTGAAGAAACTGCATCTTGTTTTTACTTCATGTATCAGGTTTGCTTTTGTTAAATTTCAATTTAAAATAGTGTTTTCTGGCACTTTTGTGTGGTTTTGGCATTGTTATGCTGTTTTTTCTTGGAAGTTAAATGAAATACTCTCCGGCCCCGTCCAGATAGATGCAAAAACTTCACAAAATGGAGTTACTCGGCACGATTTCGTGCATATCTAGTGAAACAAACTGATATAGGTATTCAGTTCAGTAGGTTGTTGTAAGTATTAAAAAATTAAGCATTCTAACAATTTGTGAAAGGAGAATCTGATTATAATTATCTTGGAATTAAGTTCTAGAAAGCTTTCACATTAATTATCTTGTTTTCTTTATGCTTCTCAGTTCTCTCATGTGATGAATTCAGGTGATTATTACACCGTTCAATGAGCTTGAAGAAAATGAGTTCCTTGATCCAAGAACTGCACAAGTTGCTATAATAGACAGTATCAAACAGGTACGGGTTCAGTCTGAATCAATCTAAGCAGAGTAACCATTTTATGGACTTAAGAGTATAATACTTCTAAATGGCTAAACGTGAAATTATCTTTTTACTTTCTTTTGACGTGGAGAAAGGAAGAAAGCATGATCACAAGATTGTCTATTGAAGTTATTTACTTAATGTAGGAACTTGAGTATTTCTACTTTGTTTGCCATTTTAAGGTCAATACCATGCGTATTCTTAGTTGATGTTACATATCTTAGACATTGTCTGTTGATTTAATCTGTATTAACAAAATCAGAAAACCTGTCTATTTAATTATGCATGAAAACTTAGGCCAAATTGTTAGTTACATGTTATTGAGTCACGTCTTCTGATTTATTCTATTGACAATGTGGCAACTAAATTCATGAAAATTACTTACCTCTATACTTCCCTGTCCCCTCTTTCTCGTTTGAATCCAACTTTCTAAGTTGTTATATTATTCGATCTGTTTTTGTTAATTTGGTGGTTAATTGGCTTTAGGTGTGCACTGAAGTGAGACATGCGCTGGATGAAGAACTCCCCTCTGCATATGTTGAGGAATTTCGGTAGGCTTTAAAATTTGCAGTTTGAAGAACTGAAAATTAGTAACCATCCTTGTTGGAGATTTCTAATGTTAAATGATTTGGTGGATGTGAGTTTTAAAATTTGCAGCATATGAAACCACTACTTTTTAAGCTAATGGCTGATATGGGTCATGTAATAAAATATAGTAGGATCAAGCAATTAGGACTATGCGGTCTCCTTGAAATGAATAGTGCTTATATCAAGCTGTCTAAAGTTTCTCCTTTGCTGAACAACAAATATATGTACTTCTATAACATATTTGTAAAATATTGAGCCATTTTTTAGGAGACATCGACCATTCCAGGTCAAGTGAGGATCATACACCCAAAAATTGTCTTGGTTATCCCTGAAAGATGAGCCACAACCATTGATTAGACTTTGAAGAGAGTATGGAATTGAAAATATGGGACAAAATGAAACAAACTAAAGTAAATAAACCTGAACATGTCTTTTCATCATTTTGTGTTCTATGTTATGGCAGATATGCTGTAGATATGGAAGTTTCTCGATATGTTGGGGAAGCATATCCAAAAGGTGTTTGCTCAGTTTACTGTGTAAATGGAAAAGACGCAGAGGGACCTGAATCTGATTTTGAGCTTGCTGTGGTTATTGCTGCTGCTAGACATAGCCCTCAGAACTTCTGGTGCGTGCATAATCTAATCATCATTATATTCCTTAGCAACTAACTTCTTGGCTCGTTTATTTGTCTTTCATGTTCTTGTACTTTTTCTCTTTCTTTACTTTTTGTGATACGCAGGTTTTGAATAATTAATATCATCACGTGGCTATATTGAATTTATTTTAGTTACAGCATATGTGATGCTTTTGGTTCATTTGTTGCTGATGACATTTCAATTCATTAGTGTTGACGAGTCATTTTGCAGAGTAAGTTCAATTCATTTTATTTGTTGCCTGTTTACATTCTTGTGCAGTAATGGAAGCTGGCGTTCTACATGGAATATTGAGTTCAAAAGTGATTTTCAATCCCTTGAAATAAAAGGGAAAATGCAGGTATTTTCTGTTACTTAATCCAAGTGCACTATATATTTGTGTCTCTTTCTCACTCTCTCATTTCTTGGATTTCAACTTTATTTATTTTAATGTTTTATTAAAACCTCAACGTTCTGTATTTTGGGGCTCTTTTCTCTCAACTCTCTTCAGGTGGGTGCCCATTACTTTGAAGAGGGAAATGTTCAATTAGATGCGAAGCATGAATGTAACGATTCAACTGTCCTACAGGTCATTACACATTTGAAAATTTCTTTTTAATTATTATTTCTAGTTAGTATGGTTACCTTAGATGGAGTCAAACGTTTGGTTGCTTTTTTTGCTTTTTACATCAGTTTGTATAGAGCATTGGACTTCCCAATAGGCAGTGCAGCATGATCATGTGAAATGAAATTTTCATTTATTCTATCAAAATTTTCAATTTGTCCATCAGATTTTTTCATTTTTCTCAGCTTAAATAGGGCATGAGCTTGCTTTTCAAATTGTTGATATTTGTTACATTTGTTTTAGGCTCCTGAAGATTGTGCAGCCGCTATAGCCAATATTATTCGTCATCACGAAGCAGAATACTTAGTATCTCTTGAGGTTCTTTGTAAATCCCTAATCATTATTAGTTATTGGGGTTTCTAGGAATTTTAACTAATGCTTCTTTGTTCGACAGACATCCTATTCAAACTTGCCAGATACCACCTTCAAGGTACTATTTTGCTCTTGGGTGAAAAGCTTTTGCATTTCTAGAGCCCTTCATTGGAGTAAAATTTGTTTTGTTATGTGAAATGAATTTCGTCTGTTATCCCATAATCTTTTCACCATCTGATCCTAGTAAAAGTTGGAGGGAGAGAAATTTTAAAAATATTCTTGACTCAAGGAAGTGAAGAAGTCATTTTGATCTTTATCTTATTTTTGTCCTGAACTTTAAATATGATAATAATAATACTAATAACTCAAAAAATAGAATGAGTTTGTCAATCCTATGCAATGTCTTGGAAAAATTACTTTTATCATTTTCAAAACCACTCCAAAAATGTTTTTATTCATTCCAAATCAATTTTAATATCATGAAAATTGCGTTTAAAAGTGTGTAAATTATACATTTAATTGATTTTGAGTGATTAAAGGTGTGTTTCGGAGTAATTTTAAACATGACAAAAATGCTTTTAACCATTCTAAAATTACTCCCAAGCATGCCTACTTCATTGATTTTAACCATCTTATCGTGCATTGTAGGATCTTCGAAGGAAGCTTCCCGTTACTCGGACCCTATTTCCATGGCACAATACCTCACAATTCAGCTTAACAAGAGACATTGCTAAAGAACTCGGAATTGGAAAGTAAATCCACAAACATCAAAATCTCAGTATCTTTGTTCACTTTTACAGGTAAAAAACAAAGACACTATTCTCATTCCATTCCCCTTTCGTTTGCTCCTAAATCACATAACTCTGCCTTTAGAGTTAGTTTCCTTATTTTTTTCCACTTTCCTCGATATTTGGTTCCTCGTTTCGATGTCTTCCTTGTAGTAGTATTCATTCTTGTGAGTTGTATAGCTGCGAGCGCAACAGGTGCGTGCTACCGTTTGTAAGTACTTACTTATAATTTGTAAAGTGATGGTTATGTGTTTTTGTTTGATGTATTGCATTTGATTCTGAAATAGATTGTACAAGTGTTTGCTTCCTTCTTCTAGTTCTTTTCACCTTCTATTTGCCTTTTTGTTTTTCTTACTTCATTAACAGTCCATCAGTGCTTGTTTGTTTAAAATTCCCTGACAAAGACCATATAATTCTAATTCCTCTTTACTTTTG

mRNA sequence

CGCTCCACCCTCTCTCCCTCCTTCAGTTTCAGTTCATTTGAAAAATCGGCGAAACGAATTTCTATTTTTCGGCAATGGCCGATGAAGAAGTGGAGCTCAGCGACGAGCAGAAGAAGGAGATCGCGAAGTGGTTTCTCCTAAACGCTCCAGCCGGAGAAATCCAATTCGTCGCCAAAGATGTGAAGAAAATTTTGAACGACGATGAGTTGTACCACGAGGCAGCATCTGAAGCTTTTCCTCAGTACAATAAATCACATATGATTTGTATTGAAATGCCTGGTCGAGTAGGAGATGTGATTATTACACCGTTCAATGAGCTTGAAGAAAATGAGTTCCTTGATCCAAGAACTGCACAAGTTGCTATAATAGACAGTATCAAACAGGTGTGCACTGAAGTGAGACATGCGCTGGATGAAGAACTCCCCTCTGCATATGTTGAGGAATTTCGATATGCTGTAGATATGGAAGTTTCTCGATATGTTGGGGAAGCATATCCAAAAGGTGTTTGCTCAGTTTACTGTGTAAATGGAAAAGACGCAGAGGGACCTGAATCTGATTTTGAGCTTGCTGTGGTTATTGCTGCTGCTAGACATAGCCCTCAGAACTTCTGTAATGGAAGCTGGCGTTCTACATGGAATATTGAGTTCAAAAGTGATTTTCAATCCCTTGAAATAAAAGGGAAAATGCAGGTGGGTGCCCATTACTTTGAAGAGGGAAATGTTCAATTAGATGCGAAGCATGAATGTAACGATTCAACTGTCCTACAGGCTCCTGAAGATTGTGCAGCCGCTATAGCCAATATTATTCGTCATCACGAAGCAGAATACTTAGTATCTCTTGAGACATCCTATTCAAACTTGCCAGATACCACCTTCAAGGATCTTCGAAGGAAGCTTCCCGTTACTCGGACCCTATTTCCATGGCACAATACCTCACAATTCAGCTTAACAAGAGACATTGCTAAAGAACTCGGAATTGGAAAGTAAATCCACAAACATCAAAATCTCAGTATCTTTGTTCACTTTTACAGGTAAAAAACAAAGACACTATTCTCATTCCATTCCCCTTTCGTTTGCTCCTAAATCACATAACTCTGCCTTTAGAGTTAGTTTCCTTATTTTTTTCCACTTTCCTCGATATTTGGTTCCTCGTTTCGATGTCTTCCTTGTAGTAGTATTCATTCTTGTGAGTTGTATAGCTGCGAGCGCAACAGGTGCGTGCTACCGTTTGTAAGTACTTACTTATAATTTGTAAAGTGATGGTTATGTGTTTTTGTTTGATGTATTGCATTTGATTCTGAAATAGATTGTACAAGTGTTTGCTTCCTTCTTCTAGTTCTTTTCACCTTCTATTTGCCTTTTTGTTTTTCTTACTTCATTAACAGTCCATCAGTGCTTGTTTGTTTAAAATTCCCTGACAAAGACCATATAATTCTAATTCCTCTTTACTTTTG

Coding sequence (CDS)

ATGGCCGATGAAGAAGTGGAGCTCAGCGACGAGCAGAAGAAGGAGATCGCGAAGTGGTTTCTCCTAAACGCTCCAGCCGGAGAAATCCAATTCGTCGCCAAAGATGTGAAGAAAATTTTGAACGACGATGAGTTGTACCACGAGGCAGCATCTGAAGCTTTTCCTCAGTACAATAAATCACATATGATTTGTATTGAAATGCCTGGTCGAGTAGGAGATGTGATTATTACACCGTTCAATGAGCTTGAAGAAAATGAGTTCCTTGATCCAAGAACTGCACAAGTTGCTATAATAGACAGTATCAAACAGGTGTGCACTGAAGTGAGACATGCGCTGGATGAAGAACTCCCCTCTGCATATGTTGAGGAATTTCGATATGCTGTAGATATGGAAGTTTCTCGATATGTTGGGGAAGCATATCCAAAAGGTGTTTGCTCAGTTTACTGTGTAAATGGAAAAGACGCAGAGGGACCTGAATCTGATTTTGAGCTTGCTGTGGTTATTGCTGCTGCTAGACATAGCCCTCAGAACTTCTGTAATGGAAGCTGGCGTTCTACATGGAATATTGAGTTCAAAAGTGATTTTCAATCCCTTGAAATAAAAGGGAAAATGCAGGTGGGTGCCCATTACTTTGAAGAGGGAAATGTTCAATTAGATGCGAAGCATGAATGTAACGATTCAACTGTCCTACAGGCTCCTGAAGATTGTGCAGCCGCTATAGCCAATATTATTCGTCATCACGAAGCAGAATACTTAGTATCTCTTGAGACATCCTATTCAAACTTGCCAGATACCACCTTCAAGGATCTTCGAAGGAAGCTTCCCGTTACTCGGACCCTATTTCCATGGCACAATACCTCACAATTCAGCTTAACAAGAGACATTGCTAAAGAACTCGGAATTGGAAAGTAA

Protein sequence

MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKSHMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDSIKQVCTEVRHALDEELPSAYVEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSPQNFCNGSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPEDCAAAIANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELGIGK*
Homology
BLAST of CSPI02G04380 vs. ExPASy Swiss-Prot
Match: O82631 (F-actin-capping protein subunit alpha OS=Arabidopsis thaliana OX=3702 GN=At3g05520 PE=2 SV=2)

HSP 1 Score: 462.2 bits (1188), Expect = 4.5e-129
Identity = 215/308 (69.81%), Postives = 257/308 (83.44%), Query Frame = 0

Query: 1   MADE-----EVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFP 60
           MADE     E ELS +QKKEIAKWF LNAPAGEI +VAKD+K +L+D+E+Y+EAA EAFP
Sbjct: 1   MADEEDELLETELSYDQKKEIAKWFFLNAPAGEINYVAKDLKAVLSDEEVYNEAAMEAFP 60

Query: 61  QYNKSHMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDSIKQVCTEVRHALDEE 120
            YNK+HMIC+EMP   GDVI++ ++E+ ENE+LDPRTAQVAI+D +KQ+CT+VR A DEE
Sbjct: 61  VYNKTHMICLEMPSGAGDVIVSSYSEINENEYLDPRTAQVAIVDHVKQICTKVRPANDEE 120

Query: 121 LPSAYVEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSP 180
           LPS Y+EE+RYA+D E+ RYV E+YPKG+ +V CV GKD EGP SDFEL V+I A R SP
Sbjct: 121 LPSLYIEEYRYALDAEIQRYVSESYPKGMSAVNCVKGKDNEGPGSDFELVVIITAMRLSP 180

Query: 181 QNFCNGSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPED 240
           QNFCNGSWRS WNI+F+ + Q L+IKGK+QVGAHYFEEGNV+LDAK +  DST+ Q+ +D
Sbjct: 181 QNFCNGSWRSVWNIDFQDESQVLDIKGKLQVGAHYFEEGNVELDAKKDFQDSTIFQSADD 240

Query: 241 CAAAIANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDI 300
           CA AIANIIRHHE EYL SLE +YS LPD TFKDLRRKLPVTRTLFPW NT QFSLTR++
Sbjct: 241 CAIAIANIIRHHETEYLASLEVAYSKLPDNTFKDLRRKLPVTRTLFPWQNTLQFSLTREV 300

Query: 301 AKELGIGK 304
            KELG+GK
Sbjct: 301 EKELGLGK 308

BLAST of CSPI02G04380 vs. ExPASy Swiss-Prot
Match: Q9W2N0 (F-actin-capping protein subunit alpha OS=Drosophila melanogaster OX=7227 GN=cpa PE=2 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 5.6e-47
Identity = 101/292 (34.59%), Postives = 163/292 (55.82%), Query Frame = 0

Query: 4   EEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKSHMI 63
           E+  ++D +K  I   F+L+AP GE   V  DV+++L +D L  + AS AF QYNK  + 
Sbjct: 2   EQTPITDAEKVRIVSDFILHAPPGEFNEVFNDVRELLKNDTLLKDGASHAFAQYNKDQLT 61

Query: 64  CIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDSIKQVCTEVRHALDEELPSAYVEE 123
            + + G   + II+  N+L    F DPRT Q    D +++  ++ +   D E   A  E 
Sbjct: 62  PVRIEGTDHNAIISEHNDLGNGRFYDPRTKQAFKYDHLRKEASDYQ---DVE-ADATAEP 121

Query: 124 FRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSPQNFCNGSW 183
           +R A+D+E   Y    Y  GV SV+   GK A+G  +   L + I   +  P+N+ NG W
Sbjct: 122 WRAALDLETLAYTASHYRHGVSSVF---GK-AQG--NQITLTICIEDHQFQPKNYWNGRW 181

Query: 184 RSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPEDCAAAIANI 243
           RS W++ F++   + E+KG ++V  HY+E+GNVQL +  EC +S V+   +  A  +  +
Sbjct: 182 RSQWHVTFQAGSGTAELKGVLKVQVHYYEDGNVQLVSSKECRESVVVSNEQQVAKEVIRL 241

Query: 244 IRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDI 296
           I   E EY +++  +Y  + DTTFK +RR+LP+TRT   W     +S+ +++
Sbjct: 242 IEDAENEYQLAISENYQTMSDTTFKAMRRQLPITRTKIDWSKIVSYSIGKEL 283

BLAST of CSPI02G04380 vs. ExPASy Swiss-Prot
Match: Q00PJ7 (F-actin-capping protein subunit alpha-2 OS=Atelerix albiventris OX=9368 GN=CAPZA2 PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.1e-45
Identity = 103/295 (34.92%), Postives = 158/295 (53.56%), Query Frame = 0

Query: 1   MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS 60
           MAD E +LSDE+K  IA  F+++AP GE   V  DV+ +LN+D L  E A+ AF QYN  
Sbjct: 1   MADLEEQLSDEEKVRIAAKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNLD 60

Query: 61  HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDSIKQVCTEVRHALDEELPSAY 120
               +++ G    V+IT   +L   +FLDP+       D +++  T+ R    E      
Sbjct: 61  QFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATDPRPYEAENT---- 120

Query: 121 VEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSPQNFCN 180
           VE +R +V+  +  YV E YP GVC+VY   GK  +G ++   +   I + +   +NF N
Sbjct: 121 VESWRTSVETALRAYVKEHYPNGVCTVY---GKKIDGQQT---IIACIESHQFQAKNFWN 180

Query: 181 GSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPEDCAAAI 240
           G WRS W  +F     S ++ G +++  HY+E+GNVQL +  +  DS  +      A   
Sbjct: 181 GRWRSEW--KFTISPSSTQVVGILKIQVHYYEDGNVQLVSHKDIQDSLTVSNEAQTAKEF 240

Query: 241 ANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDI 296
             I+   E EY  ++  +Y  + DTTFK LRR+LPVTRT   W+    + + +++
Sbjct: 241 IKIVEAAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEM 283

BLAST of CSPI02G04380 vs. ExPASy Swiss-Prot
Match: P28497 (F-actin-capping protein subunit alpha-2 OS=Gallus gallus OX=9031 GN=CAPZA2 PE=1 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 5.2e-45
Identity = 102/295 (34.58%), Postives = 161/295 (54.58%), Query Frame = 0

Query: 1   MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS 60
           MAD E +LSDE+K  IA  F+++AP GE   V  DV+ +LN+D L  E A+ AF QYN  
Sbjct: 1   MADLEEQLSDEEKVRIAAKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNLD 60

Query: 61  HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDSIKQVCTEVRHALDEELPSAY 120
               +++ G    V+IT   +L   +FLDP+       D +++  T+ R     E+ +A 
Sbjct: 61  QFTPVKIDGYDEQVLITEHGDLGNGKFLDPKNKISFKFDHLRKEATDPR---PHEVENA- 120

Query: 121 VEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSPQNFCN 180
           +E +R +V+  +  YV E YP GVC+VY   GK  +G ++   +   I + +   +NF N
Sbjct: 121 IESWRNSVETAMKAYVKEHYPNGVCTVY---GKTIDGQQT---IIACIESHQFQAKNFWN 180

Query: 181 GSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPEDCAAAI 240
           G WRS W  +F     + ++ G +++  HY+E+GNVQL +  +  DS  +      A   
Sbjct: 181 GRWRSEW--KFTISPSTTQVAGILKIQVHYYEDGNVQLVSHKDIQDSLTVSNEAQTAKEF 240

Query: 241 ANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDI 296
             I+   E EY  ++  +Y  + DTTFK LRR+LPVTRT   W+    + + +++
Sbjct: 241 IKIVEAAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEM 283

BLAST of CSPI02G04380 vs. ExPASy Swiss-Prot
Match: Q2IBA7 (F-actin-capping protein subunit alpha-2 OS=Chlorocebus aethiops OX=9534 GN=CAPZA2 PE=3 SV=3)

HSP 1 Score: 183.0 bits (463), Expect = 5.2e-45
Identity = 103/295 (34.92%), Postives = 161/295 (54.58%), Query Frame = 0

Query: 1   MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS 60
           MAD E +LSDE+K  IA  F+++AP GE   V  DV+ +LN+D L  E A+ AF QYN  
Sbjct: 1   MADLEEQLSDEEKVRIAAKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNLD 60

Query: 61  HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDSIKQVCTEVRHALDEELPSAY 120
               +++ G    V+IT   +L   +FLDP+       D +++  T+ R     E+ +A 
Sbjct: 61  QFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATDPRPC---EVENA- 120

Query: 121 VEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSPQNFCN 180
           VE +R +V+  +  YV E YP GVC+VY   GK  +G ++   +   I + +   +NF N
Sbjct: 121 VESWRTSVETALRAYVKEHYPNGVCTVY---GKKIDGQQT---IIACIESHQFQAKNFWN 180

Query: 181 GSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPEDCAAAI 240
           G WRS W  +F     + ++ G +++  HY+E+GNVQL +  +  DS  +      A   
Sbjct: 181 GRWRSEW--KFTITPSTTQVVGILKIQVHYYEDGNVQLVSHKDIQDSLTVSNEVQTAKEF 240

Query: 241 ANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDI 296
             I+   E EY  ++  +Y  + DTTFK LRR+LPVTRT   W+    + + +++
Sbjct: 241 IKIVEAAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEM 283

BLAST of CSPI02G04380 vs. ExPASy TrEMBL
Match: A0A0A0LIW0 (F-actin-capping protein subunit alpha OS=Cucumis sativus OX=3659 GN=Csa_2G031720 PE=3 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 1.6e-174
Identity = 302/303 (99.67%), Postives = 303/303 (100.00%), Query Frame = 0

Query: 1   MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS 60
           MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS
Sbjct: 1   MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS 60

Query: 61  HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDSIKQVCTEVRHALDEELPSAY 120
           HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIID+IKQVCTEVRHALDEELPSAY
Sbjct: 61  HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDNIKQVCTEVRHALDEELPSAY 120

Query: 121 VEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSPQNFCN 180
           VEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSPQNFCN
Sbjct: 121 VEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSPQNFCN 180

Query: 181 GSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPEDCAAAI 240
           GSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPEDCAAAI
Sbjct: 181 GSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPEDCAAAI 240

Query: 241 ANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG 300
           ANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG
Sbjct: 241 ANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG 300

Query: 301 IGK 304
           IGK
Sbjct: 301 IGK 303

BLAST of CSPI02G04380 vs. ExPASy TrEMBL
Match: A0A5A7TYJ5 (F-actin-capping protein subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001440 PE=3 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 3.7e-171
Identity = 295/303 (97.36%), Postives = 298/303 (98.35%), Query Frame = 0

Query: 1   MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS 60
           MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS
Sbjct: 1   MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS 60

Query: 61  HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDSIKQVCTEVRHALDEELPSAY 120
           HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIID+IKQVCTEVRHALDEELPSAY
Sbjct: 61  HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDNIKQVCTEVRHALDEELPSAY 120

Query: 121 VEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSPQNFCN 180
           VEEFR AVDMEVSRYVGEAYPKGVCSVYCVNGKD EGPESDFELAVVIAAARHSPQNFCN
Sbjct: 121 VEEFRCAVDMEVSRYVGEAYPKGVCSVYCVNGKDVEGPESDFELAVVIAAARHSPQNFCN 180

Query: 181 GSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPEDCAAAI 240
           GSWRSTWNIEFK DFQSLEI+GKMQVGAHYFEEGNVQLDAKHECNDST+LQAPEDCA AI
Sbjct: 181 GSWRSTWNIEFKGDFQSLEIRGKMQVGAHYFEEGNVQLDAKHECNDSTILQAPEDCAVAI 240

Query: 241 ANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG 300
           ANIIRHHEAEYL SLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG
Sbjct: 241 ANIIRHHEAEYLASLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG 300

Query: 301 IGK 304
           IGK
Sbjct: 301 IGK 303

BLAST of CSPI02G04380 vs. ExPASy TrEMBL
Match: A0A6J1G3Y3 (F-actin-capping protein subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111450528 PE=3 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 8.3e-163
Identity = 280/303 (92.41%), Postives = 293/303 (96.70%), Query Frame = 0

Query: 1   MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS 60
           MAD+EVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDD+LYHEAASEAFPQYNKS
Sbjct: 1   MADDEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDKLYHEAASEAFPQYNKS 60

Query: 61  HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDSIKQVCTEVRHALDEELPSAY 120
           HMI +EMPG VGDVIITPFNELEENEFLDPRT QVAIID+IKQVCT VR ALDEELPSAY
Sbjct: 61  HMISLEMPGLVGDVIITPFNELEENEFLDPRTTQVAIIDNIKQVCTAVRPALDEELPSAY 120

Query: 121 VEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSPQNFCN 180
           VE+FR AVD E+++YVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVI+A+RHSPQNFCN
Sbjct: 121 VEDFRCAVDEEITQYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVISASRHSPQNFCN 180

Query: 181 GSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPEDCAAAI 240
           GSWRSTWNIEFKSDFQSLEI+GKMQV AHYFEEGNVQLDAKHECNDST+LQAPEDCA AI
Sbjct: 181 GSWRSTWNIEFKSDFQSLEIRGKMQVVAHYFEEGNVQLDAKHECNDSTILQAPEDCAVAI 240

Query: 241 ANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG 300
           ANIIRHHEAEYL SLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG
Sbjct: 241 ANIIRHHEAEYLASLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG 300

Query: 301 IGK 304
           +GK
Sbjct: 301 VGK 303

BLAST of CSPI02G04380 vs. ExPASy TrEMBL
Match: A0A6J1KFA0 (F-actin-capping protein subunit alpha OS=Cucurbita maxima OX=3661 GN=LOC111493782 PE=3 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.4e-162
Identity = 280/303 (92.41%), Postives = 292/303 (96.37%), Query Frame = 0

Query: 1   MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS 60
           MAD+EVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDD+LYHEAASEAFPQYNKS
Sbjct: 1   MADDEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDKLYHEAASEAFPQYNKS 60

Query: 61  HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDSIKQVCTEVRHALDEELPSAY 120
           HMI +EMPG VGDVIITPFNELEENEFLDPRT QVAIID+IKQVCT VR ALDEELPSAY
Sbjct: 61  HMISLEMPGLVGDVIITPFNELEENEFLDPRTTQVAIIDNIKQVCTAVRPALDEELPSAY 120

Query: 121 VEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSPQNFCN 180
           VE+FR AVD E++RYVGEAYPKGVCSVYCV GKDAEGPESDFELAVVI+A+RHSPQNFCN
Sbjct: 121 VEDFRCAVDEEIARYVGEAYPKGVCSVYCVTGKDAEGPESDFELAVVISASRHSPQNFCN 180

Query: 181 GSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPEDCAAAI 240
           GSWRSTWNIEFKSDFQSLEI+GKMQV AHYFEEGNVQLDAKHECNDST+LQAPEDCA AI
Sbjct: 181 GSWRSTWNIEFKSDFQSLEIRGKMQVVAHYFEEGNVQLDAKHECNDSTILQAPEDCAVAI 240

Query: 241 ANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG 300
           ANIIRHHEAEYL SLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG
Sbjct: 241 ANIIRHHEAEYLASLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG 300

Query: 301 IGK 304
           +GK
Sbjct: 301 VGK 303

BLAST of CSPI02G04380 vs. ExPASy TrEMBL
Match: A0A6J1DQM2 (F-actin-capping protein subunit alpha OS=Momordica charantia OX=3673 GN=LOC111023329 PE=3 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 1.4e-157
Identity = 266/303 (87.79%), Postives = 287/303 (94.72%), Query Frame = 0

Query: 1   MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS 60
           MADEEVEL+DEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS
Sbjct: 1   MADEEVELNDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS 60

Query: 61  HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDSIKQVCTEVRHALDEELPSAY 120
           HM+CIEMPG +GDVI+TPFNELEENEFLDPRTAQVAIID+IKQVCTEVR ALDEELPSAY
Sbjct: 61  HMVCIEMPGLLGDVILTPFNELEENEFLDPRTAQVAIIDNIKQVCTEVRPALDEELPSAY 120

Query: 121 VEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSPQNFCN 180
           VEEFR +++ E++RYVGE YPKGV S+YCVNGKDA+GPE DFEL VVI+A RHSPQNFCN
Sbjct: 121 VEEFRGSIEAEIARYVGEMYPKGVYSIYCVNGKDADGPEFDFELVVVISATRHSPQNFCN 180

Query: 181 GSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPEDCAAAI 240
           GSWRSTWNIEFK+DFQ++EI+GKMQV AHYFEEGNVQLD KHECNDST++Q+PEDCA A+
Sbjct: 181 GSWRSTWNIEFKNDFQTIEIRGKMQVVAHYFEEGNVQLDTKHECNDSTIMQSPEDCAVAV 240

Query: 241 ANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG 300
           ANIIRHHEAEY  SLETSYSNLPDTTFKDLRRKLPVTRTLFPWHN SQFSLTRDIAKELG
Sbjct: 241 ANIIRHHEAEYSASLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNASQFSLTRDIAKELG 300

Query: 301 IGK 304
            GK
Sbjct: 301 AGK 303

BLAST of CSPI02G04380 vs. NCBI nr
Match: XP_004144774.1 (F-actin-capping protein subunit alpha [Cucumis sativus] >KGN60964.1 hypothetical protein Csa_021174 [Cucumis sativus])

HSP 1 Score: 621.7 bits (1602), Expect = 3.3e-174
Identity = 302/303 (99.67%), Postives = 303/303 (100.00%), Query Frame = 0

Query: 1   MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS 60
           MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS
Sbjct: 1   MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS 60

Query: 61  HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDSIKQVCTEVRHALDEELPSAY 120
           HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIID+IKQVCTEVRHALDEELPSAY
Sbjct: 61  HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDNIKQVCTEVRHALDEELPSAY 120

Query: 121 VEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSPQNFCN 180
           VEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSPQNFCN
Sbjct: 121 VEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSPQNFCN 180

Query: 181 GSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPEDCAAAI 240
           GSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPEDCAAAI
Sbjct: 181 GSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPEDCAAAI 240

Query: 241 ANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG 300
           ANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG
Sbjct: 241 ANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG 300

Query: 301 IGK 304
           IGK
Sbjct: 301 IGK 303

BLAST of CSPI02G04380 vs. NCBI nr
Match: KAA0048038.1 (F-actin-capping protein subunit alpha [Cucumis melo var. makuwa] >TYJ96498.1 F-actin-capping protein subunit alpha [Cucumis melo var. makuwa])

HSP 1 Score: 610.5 bits (1573), Expect = 7.7e-171
Identity = 295/303 (97.36%), Postives = 298/303 (98.35%), Query Frame = 0

Query: 1   MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS 60
           MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS
Sbjct: 1   MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS 60

Query: 61  HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDSIKQVCTEVRHALDEELPSAY 120
           HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIID+IKQVCTEVRHALDEELPSAY
Sbjct: 61  HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDNIKQVCTEVRHALDEELPSAY 120

Query: 121 VEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSPQNFCN 180
           VEEFR AVDMEVSRYVGEAYPKGVCSVYCVNGKD EGPESDFELAVVIAAARHSPQNFCN
Sbjct: 121 VEEFRCAVDMEVSRYVGEAYPKGVCSVYCVNGKDVEGPESDFELAVVIAAARHSPQNFCN 180

Query: 181 GSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPEDCAAAI 240
           GSWRSTWNIEFK DFQSLEI+GKMQVGAHYFEEGNVQLDAKHECNDST+LQAPEDCA AI
Sbjct: 181 GSWRSTWNIEFKGDFQSLEIRGKMQVGAHYFEEGNVQLDAKHECNDSTILQAPEDCAVAI 240

Query: 241 ANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG 300
           ANIIRHHEAEYL SLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG
Sbjct: 241 ANIIRHHEAEYLASLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG 300

Query: 301 IGK 304
           IGK
Sbjct: 301 IGK 303

BLAST of CSPI02G04380 vs. NCBI nr
Match: XP_038889650.1 (F-actin-capping protein subunit alpha [Benincasa hispida])

HSP 1 Score: 589.7 bits (1519), Expect = 1.4e-164
Identity = 286/303 (94.39%), Postives = 293/303 (96.70%), Query Frame = 0

Query: 1   MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS 60
           MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS
Sbjct: 1   MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS 60

Query: 61  HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDSIKQVCTEVRHALDEELPSAY 120
           HMI IEMPGRVGDVIITPFNELEENEFLDPRTAQVAIID+IKQ C  VR ALDEELPSAY
Sbjct: 61  HMIFIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDNIKQACAGVRPALDEELPSAY 120

Query: 121 VEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSPQNFCN 180
           VEEFR AVD E++RYVGEAYPKGVCSV+CVNGKDAEGPESDFELAVVIAAARHSPQNFCN
Sbjct: 121 VEEFRCAVDTEIARYVGEAYPKGVCSVFCVNGKDAEGPESDFELAVVIAAARHSPQNFCN 180

Query: 181 GSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPEDCAAAI 240
           GSWRSTWNIE KSDFQ+LEI+GKMQVGAHYFEEGNVQLDAKHECNDST+LQAPEDCA AI
Sbjct: 181 GSWRSTWNIECKSDFQALEIRGKMQVGAHYFEEGNVQLDAKHECNDSTILQAPEDCAVAI 240

Query: 241 ANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG 300
           ANIIRHHEAEYL SLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG
Sbjct: 241 ANIIRHHEAEYLASLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG 300

Query: 301 IGK 304
           IGK
Sbjct: 301 IGK 303

BLAST of CSPI02G04380 vs. NCBI nr
Match: KAG6599208.1 (F-actin-capping protein subunit alpha, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 584.3 bits (1505), Expect = 5.9e-163
Identity = 281/303 (92.74%), Postives = 293/303 (96.70%), Query Frame = 0

Query: 1   MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS 60
           MAD+EVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDD+LYHEAASEAFPQYNKS
Sbjct: 1   MADDEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDKLYHEAASEAFPQYNKS 60

Query: 61  HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDSIKQVCTEVRHALDEELPSAY 120
           HMI +EMPG VGDVIITPFNELEENEFLDPRT QVAIID+IKQVCT VR ALDEELPSAY
Sbjct: 61  HMISLEMPGLVGDVIITPFNELEENEFLDPRTTQVAIIDNIKQVCTAVRPALDEELPSAY 120

Query: 121 VEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSPQNFCN 180
           VE+FR AVD E++RYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVI+A+RHSPQNFCN
Sbjct: 121 VEDFRCAVDEEIARYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVISASRHSPQNFCN 180

Query: 181 GSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPEDCAAAI 240
           GSWRSTWNIEFKSDFQSLEI+GKMQV AHYFEEGNVQLDAKHECNDST+LQAPEDCA AI
Sbjct: 181 GSWRSTWNIEFKSDFQSLEIRGKMQVVAHYFEEGNVQLDAKHECNDSTILQAPEDCAVAI 240

Query: 241 ANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG 300
           ANIIRHHEAEYL SLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG
Sbjct: 241 ANIIRHHEAEYLASLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG 300

Query: 301 IGK 304
           +GK
Sbjct: 301 VGK 303

BLAST of CSPI02G04380 vs. NCBI nr
Match: XP_023545302.1 (F-actin-capping protein subunit alpha-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 583.6 bits (1503), Expect = 1.0e-162
Identity = 280/303 (92.41%), Postives = 293/303 (96.70%), Query Frame = 0

Query: 1   MADEEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFPQYNKS 60
           MAD+EVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDD+LYHEAASEAFPQYNKS
Sbjct: 1   MADDEVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDKLYHEAASEAFPQYNKS 60

Query: 61  HMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDSIKQVCTEVRHALDEELPSAY 120
           HMI +EMPG VGDVIITPFNELEENEFLDPRT QVAIID+IKQVCT VR A+DEELPSAY
Sbjct: 61  HMISLEMPGLVGDVIITPFNELEENEFLDPRTTQVAIIDNIKQVCTAVRPAMDEELPSAY 120

Query: 121 VEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSPQNFCN 180
           VE+FR AVD E++RYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVI+A+RHSPQNFCN
Sbjct: 121 VEDFRCAVDEEIARYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVISASRHSPQNFCN 180

Query: 181 GSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPEDCAAAI 240
           GSWRSTWNIEFKSDFQSLEI+GKMQV AHYFEEGNVQLDAKHECNDST+LQAPEDCA AI
Sbjct: 181 GSWRSTWNIEFKSDFQSLEIRGKMQVVAHYFEEGNVQLDAKHECNDSTILQAPEDCAVAI 240

Query: 241 ANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG 300
           ANIIRHHEAEYL SLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG
Sbjct: 241 ANIIRHHEAEYLASLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDIAKELG 300

Query: 301 IGK 304
           +GK
Sbjct: 301 VGK 303

BLAST of CSPI02G04380 vs. TAIR 10
Match: AT3G05520.1 (Subunits of heterodimeric actin filament capping protein Capz superfamily )

HSP 1 Score: 462.2 bits (1188), Expect = 3.2e-130
Identity = 215/308 (69.81%), Postives = 257/308 (83.44%), Query Frame = 0

Query: 1   MADE-----EVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFP 60
           MADE     E ELS +QKKEIAKWF LNAPAGEI +VAKD+K +L+D+E+Y+EAA EAFP
Sbjct: 1   MADEEDELLETELSYDQKKEIAKWFFLNAPAGEINYVAKDLKAVLSDEEVYNEAAMEAFP 60

Query: 61  QYNKSHMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDSIKQVCTEVRHALDEE 120
            YNK+HMIC+EMP   GDVI++ ++E+ ENE+LDPRTAQVAI+D +KQ+CT+VR A DEE
Sbjct: 61  VYNKTHMICLEMPSGAGDVIVSSYSEINENEYLDPRTAQVAIVDHVKQICTKVRPANDEE 120

Query: 121 LPSAYVEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSP 180
           LPS Y+EE+RYA+D E+ RYV E+YPKG+ +V CV GKD EGP SDFEL V+I A R SP
Sbjct: 121 LPSLYIEEYRYALDAEIQRYVSESYPKGMSAVNCVKGKDNEGPGSDFELVVIITAMRLSP 180

Query: 181 QNFCNGSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPED 240
           QNFCNGSWRS WNI+F+ + Q L+IKGK+QVGAHYFEEGNV+LDAK +  DST+ Q+ +D
Sbjct: 181 QNFCNGSWRSVWNIDFQDESQVLDIKGKLQVGAHYFEEGNVELDAKKDFQDSTIFQSADD 240

Query: 241 CAAAIANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDI 300
           CA AIANIIRHHE EYL SLE +YS LPD TFKDLRRKLPVTRTLFPW NT QFSLTR++
Sbjct: 241 CAIAIANIIRHHETEYLASLEVAYSKLPDNTFKDLRRKLPVTRTLFPWQNTLQFSLTREV 300

Query: 301 AKELGIGK 304
            KELG+GK
Sbjct: 301 EKELGLGK 308

BLAST of CSPI02G04380 vs. TAIR 10
Match: AT3G05520.2 (Subunits of heterodimeric actin filament capping protein Capz superfamily )

HSP 1 Score: 462.2 bits (1188), Expect = 3.2e-130
Identity = 215/308 (69.81%), Postives = 257/308 (83.44%), Query Frame = 0

Query: 1   MADE-----EVELSDEQKKEIAKWFLLNAPAGEIQFVAKDVKKILNDDELYHEAASEAFP 60
           MADE     E ELS +QKKEIAKWF LNAPAGEI +VAKD+K +L+D+E+Y+EAA EAFP
Sbjct: 1   MADEEDELLETELSYDQKKEIAKWFFLNAPAGEINYVAKDLKAVLSDEEVYNEAAMEAFP 60

Query: 61  QYNKSHMICIEMPGRVGDVIITPFNELEENEFLDPRTAQVAIIDSIKQVCTEVRHALDEE 120
            YNK+HMIC+EMP   GDVI++ ++E+ ENE+LDPRTAQVAI+D +KQ+CT+VR A DEE
Sbjct: 61  VYNKTHMICLEMPSGAGDVIVSSYSEINENEYLDPRTAQVAIVDHVKQICTKVRPANDEE 120

Query: 121 LPSAYVEEFRYAVDMEVSRYVGEAYPKGVCSVYCVNGKDAEGPESDFELAVVIAAARHSP 180
           LPS Y+EE+RYA+D E+ RYV E+YPKG+ +V CV GKD EGP SDFEL V+I A R SP
Sbjct: 121 LPSLYIEEYRYALDAEIQRYVSESYPKGMSAVNCVKGKDNEGPGSDFELVVIITAMRLSP 180

Query: 181 QNFCNGSWRSTWNIEFKSDFQSLEIKGKMQVGAHYFEEGNVQLDAKHECNDSTVLQAPED 240
           QNFCNGSWRS WNI+F+ + Q L+IKGK+QVGAHYFEEGNV+LDAK +  DST+ Q+ +D
Sbjct: 181 QNFCNGSWRSVWNIDFQDESQVLDIKGKLQVGAHYFEEGNVELDAKKDFQDSTIFQSADD 240

Query: 241 CAAAIANIIRHHEAEYLVSLETSYSNLPDTTFKDLRRKLPVTRTLFPWHNTSQFSLTRDI 300
           CA AIANIIRHHE EYL SLE +YS LPD TFKDLRRKLPVTRTLFPW NT QFSLTR++
Sbjct: 241 CAIAIANIIRHHETEYLASLEVAYSKLPDNTFKDLRRKLPVTRTLFPWQNTLQFSLTREV 300

Query: 301 AKELGIGK 304
            KELG+GK
Sbjct: 301 EKELGLGK 308

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O826314.5e-12969.81F-actin-capping protein subunit alpha OS=Arabidopsis thaliana OX=3702 GN=At3g055... [more]
Q9W2N05.6e-4734.59F-actin-capping protein subunit alpha OS=Drosophila melanogaster OX=7227 GN=cpa ... [more]
Q00PJ73.1e-4534.92F-actin-capping protein subunit alpha-2 OS=Atelerix albiventris OX=9368 GN=CAPZA... [more]
P284975.2e-4534.58F-actin-capping protein subunit alpha-2 OS=Gallus gallus OX=9031 GN=CAPZA2 PE=1 ... [more]
Q2IBA75.2e-4534.92F-actin-capping protein subunit alpha-2 OS=Chlorocebus aethiops OX=9534 GN=CAPZA... [more]
Match NameE-valueIdentityDescription
A0A0A0LIW01.6e-17499.67F-actin-capping protein subunit alpha OS=Cucumis sativus OX=3659 GN=Csa_2G031720... [more]
A0A5A7TYJ53.7e-17197.36F-actin-capping protein subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A6J1G3Y38.3e-16392.41F-actin-capping protein subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111450... [more]
A0A6J1KFA01.4e-16292.41F-actin-capping protein subunit alpha OS=Cucurbita maxima OX=3661 GN=LOC11149378... [more]
A0A6J1DQM21.4e-15787.79F-actin-capping protein subunit alpha OS=Momordica charantia OX=3673 GN=LOC11102... [more]
Match NameE-valueIdentityDescription
XP_004144774.13.3e-17499.67F-actin-capping protein subunit alpha [Cucumis sativus] >KGN60964.1 hypothetical... [more]
KAA0048038.17.7e-17197.36F-actin-capping protein subunit alpha [Cucumis melo var. makuwa] >TYJ96498.1 F-a... [more]
XP_038889650.11.4e-16494.39F-actin-capping protein subunit alpha [Benincasa hispida][more]
KAG6599208.15.9e-16392.74F-actin-capping protein subunit alpha, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023545302.11.0e-16292.41F-actin-capping protein subunit alpha-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G05520.13.2e-13069.81Subunits of heterodimeric actin filament capping protein Capz superfamily [more]
AT3G05520.23.2e-13069.81Subunits of heterodimeric actin filament capping protein Capz superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002189F-actin-capping protein subunit alphaPRINTSPR00191FACTINCAPAcoord: 173..187
score: 58.0
coord: 207..220
score: 60.71
coord: 264..283
score: 72.0
IPR002189F-actin-capping protein subunit alphaPFAMPF01267F-actin_cap_Acoord: 17..295
e-value: 2.0E-77
score: 260.2
IPR002189F-actin-capping protein subunit alphaPANTHERPTHR10653F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHAcoord: 5..303
IPR042276F-actin-capping protein subunit alpha/beta, domain 2GENE3D3.90.1150.210coord: 118..270
e-value: 2.1E-53
score: 182.0
IPR042489F-actin capping protein, alpha subunit, domain 1GENE3D3.30.1140.60coord: 1..111
e-value: 1.8E-34
score: 120.1
NoneNo IPR availablePANTHERPTHR10653:SF20F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHAcoord: 5..303
IPR017865F-actin capping protein, alpha subunit, conserved sitePROSITEPS00748F_ACTIN_CAPPING_A_1coord: 208..216
IPR017865F-actin capping protein, alpha subunit, conserved sitePROSITEPS00749F_ACTIN_CAPPING_A_2coord: 268..278
IPR037282F-actin-capping protein subunit alpha/betaSUPERFAMILY90096Subunits of heterodimeric actin filament capping protein Capzcoord: 8..292

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G04380.1CSPI02G04380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051016 barbed-end actin filament capping
cellular_component GO:0008290 F-actin capping protein complex
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0051015 actin filament binding