CSPI02G04350 (gene) Cucumber (PI 183967) v1

BLAST of CSPI02G04350 vs. ExPASy Swiss-Prot
Match: Q3MKY2 (Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 1.2e-88
Identity = 164/341 (48.09%), Postives = 214/341 (62.76%), Query Frame = 0

Query: 36  CNFDGIYQLGDSISDTGNLIRENPNTP---FSHLPYGQTFFSTPTGRCSNGLLIIDYFAL 95
           C FD IYQLGDS SDTGNLIR  P+ P    +H PYG+TF  TPTGRCS+G LIID+ A 
Sbjct: 25  CPFDSIYQLGDSFSDTGNLIRLPPDGPTFTAAHFPYGETFPGTPTGRCSDGRLIIDFIAT 84

Query: 96  DARLPLVNPYLNKDALTRHGINFAVAGSTALSSELLSKKKISSLLTNSSLDLQLDWMFSH 155
              LPL+NPYL ++   RHG+NFAVAG+TAL    L+ + +     +S L  QL+W  ++
Sbjct: 85  ALNLPLLNPYLQQNVSFRHGVNFAVAGATALDRSFLAARGVQVSDIHSHLSAQLNWFRTY 144

Query: 156 FNSICYDQKDCDEKLKNALFLVGEIGANDYNYALLQGKTIEEVKEMVPEVVQAIKNAVER 215
             SIC   K+C  KLKNALF++G IG ND NYA    +TIEE++  VP + +A+ NA   
Sbjct: 145 LGSICSTPKECSNKLKNALFILGNIGNNDVNYA-FPNRTIEEIRAYVPFITEAVANATRE 204

Query: 216 VISYGATRVVVSGNFPIGCFPISLTVFQTNNTTAYDEYHCLKSLNALTSYHNDQIKQAIE 275
           +I  G +RV+V G FPIGC   +L           D+  CL SLN L+ Y N   ++A+ 
Sbjct: 205 IIRLGGSRVIVPGIFPIGCVARNLNFLNFFPDGDKDDLGCLSSLNNLSIYFNSLFQRALA 264

Query: 276 VLKKENLHTVIVYGDYYNAFLWILRRASMLGFDNGSLQKSCCGIGGDYNFDLKRTCGNNG 335
            L  E    VI+Y DYYNA+ ++ R    LG ++ SL K CCGIGG YN+D  R CG+ G
Sbjct: 265 SLSIEFPQAVIIYADYYNAWRFLFRNGPALGSNSTSLLKCCCGIGGPYNYDPDRECGSRG 324

Query: 336 VGVCPNPDKVISWDGVHLTQKAYKYIADWLILNISPKLNCT 374
           V VCPNP + I WDG H TQ AY+ +A+++I  I   L C+
Sbjct: 325 VPVCPNPTQYIQWDGTHFTQAAYRRVAEYVIPGIIKALKCS 364

BLAST of CSPI02G04350 vs. ExPASy Swiss-Prot
Match: Q8RXT9 (GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana OX=3702 GN=At1g28590 PE=2 SV=2)

HSP 1 Score: 273.5 bits (698), Expect = 3.6e-72
Identity = 147/350 (42.00%), Postives = 215/350 (61.43%), Query Frame = 0

Query: 19  FLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLI-RENPN-TPFSHL-PYGQTFFST 78
           F+   L  +S  +  +  NF  I   GDSI+DTGNL+   +PN  P S   PYG+TFF  
Sbjct: 15  FILSTLLVTSVNSQTQCRNFKSIISFGDSIADTGNLLGLSDPNDLPASAFPPYGETFFHH 74

Query: 79  PTGRCSNGLLIIDYFALDARLPLVNPYLN-KDALTRHGINFAVAGSTALSSELLSKKKIS 138
           PTGR S+G LIID+ A     PLV P+   ++A  + G+NFAVAG+TAL    L ++ I 
Sbjct: 75  PTGRYSDGRLIIDFIAEFLGFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFLEERGIH 134

Query: 139 SLLTNSSLDLQLDWMFSHFNSICYDQKDCDEKLKNALFLVGEIGANDYNYALLQGKTIEE 198
           S +TN SL +QL        ++C    DC + ++NAL L+GEIG NDYN+AL Q K ++E
Sbjct: 135 STITNVSLSVQLRSFTESLPNLCGSPSDCRDMIENALILMGEIGGNDYNFALFQRKPVKE 194

Query: 199 VKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTVFQTNNTTAYDEY-HCL 258
           V+E+VP V+  I +A+  ++  G    +V GNFPIG     LT+++T+N   YD    CL
Sbjct: 195 VEELVPFVIATISSAITELVCMGGRTFLVPGNFPIGYSASYLTLYKTSNKEEYDPLTGCL 254

Query: 259 KSLNALTSYHNDQIKQAIEVLKKENLHTVIVYGDYYNAFLWILRRASMLGFDNGSLQKSC 318
           K LN  + Y+N Q+++ +  L+K   H  I+Y DYYNA L + +  +  GF N  L  +C
Sbjct: 255 KWLNDFSEYYNKQLQEELNGLRKLYPHVNIIYADYYNALLRLFQEPAKFGFMNRPL-PAC 314

Query: 319 CGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKYIADWLI 364
           CG+GG YNF+  R CG+ GV  C +P + +++DG+H+T+ AY+ I++ L+
Sbjct: 315 CGVGGSYNFNFSRRCGSVGVEYCDDPSQYVNYDGIHMTEAAYRLISEGLL 363

BLAST of CSPI02G04350 vs. ExPASy Swiss-Prot
Match: Q9C857 (GDSL esterase/lipase At1g31550 OS=Arabidopsis thaliana OX=3702 GN=At1g31550 PE=2 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 4.7e-72
Identity = 154/350 (44.00%), Postives = 210/350 (60.00%), Query Frame = 0

Query: 20  LFLLLSPSSNAASLKAC-NFDGIYQLGDSISDTGNL--IRENPNTPFSHL-PYGQTFFST 79
           LFL     S  +S   C NF+ I   GDSI+DTGNL  + ++ N P S   PYG+TFF  
Sbjct: 15  LFLSTLFVSIVSSESQCRNFESIISFGDSIADTGNLLGLSDHNNLPMSAFPPYGETFFHH 74

Query: 80  PTGRCSNGLLIIDYFALDARLPLVNPYL-NKDALTRHGINFAVAGSTALSSELLSKKKIS 139
           PTGR S+G LIID+ A    LP V PY  + +     G+NFAVA +TAL S  L +K   
Sbjct: 75  PTGRFSDGRLIIDFIAEFLGLPYVPPYFGSTNGNFEKGVNFAVASATALESSFLEEKGY- 134

Query: 140 SLLTNSSLDLQLDWMFSHFNSICYDQKDCDEKLKNALFLVGEIGANDYNYALLQGKTIEE 199
               N SL +QL        ++C    DC + + NAL L+GEIGANDYN+   Q + ++E
Sbjct: 135 HCPHNFSLGVQLKIFKQSLPNLCGLPSDCRDMIGNALILMGEIGANDYNFPFFQLRPLDE 194

Query: 200 VKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTVFQTNNTTAYDEY-HCL 259
           VKE+VP V+  I +A+  +I  G    +V G FP+GC    LT+ QT+N   YD    CL
Sbjct: 195 VKELVPLVISTISSAITELIGMGGRTFLVPGGFPLGCSVAFLTLHQTSNMEEYDPLTGCL 254

Query: 260 KSLNALTSYHNDQIKQAIEVLKKENLHTVIVYGDYYNAFLWILRRASMLGFDNGSLQKSC 319
           K LN    YH++Q+++ +  L+K N H  I+Y DYYNA L + R  S  GF N  L  +C
Sbjct: 255 KWLNKFGEYHSEQLQEELNRLRKLNPHVNIIYADYYNASLRLGREPSKYGFINRHL-SAC 314

Query: 320 CGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKYIADWLI 364
           CG+GG YNF+L R+CG+ GV  C +P K ++WDG+H+T+ A+K +AD L+
Sbjct: 315 CGVGGPYNFNLSRSCGSVGVEACSDPSKYVAWDGLHMTEAAHKSMADGLV 362

BLAST of CSPI02G04350 vs. ExPASy Swiss-Prot
Match: Q9LZB2 (GDSL esterase/lipase At5g03980 OS=Arabidopsis thaliana OX=3702 GN=At5g03980 PE=2 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 3.1e-71
Identity = 161/370 (43.51%), Postives = 205/370 (55.41%), Query Frame = 0

Query: 5   SSSSSITSFVFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLIRENPNTPFS 64
           S++ +++  VF    LF+ L  SS+      C  + IYQ GDSISDTGNLIR  P     
Sbjct: 2   STTKALSLLVF---ILFVSLVHSSD-----QCPINSIYQFGDSISDTGNLIRNGP----- 61

Query: 65  HLPYGQTFFSTPTGRCSNGLLIIDYFALDARLPLVNPYLNKDALTRHGINFAVAGSTALS 124
                    S+PT                   P   P    +      +NF V+GSTAL+
Sbjct: 62  --------ASSPT-------------------PKPLPQREHNVF----VNFGVSGSTALN 121

Query: 125 SELLSKKKISSLLTNSSLDLQLDWMFSHFNSICY-DQKDCDEKLKNALFLVGEIGANDYN 184
           S   S++ +    TN+ L +QL W   H  S C+    DC   LK++LF+VGEIG NDYN
Sbjct: 122 SSFFSERNLHVPATNTPLSMQLAWFKGHLRSTCHGSSSDC---LKHSLFMVGEIGGNDYN 181

Query: 185 YALLQGKTIEEVKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTVFQTNN 244
           Y   QGK +EE++  +P VV AI  A   VI  GA  VVV GNFP+GCFPI LT F   +
Sbjct: 182 YGFFQGKPMEEIRSYIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKD 241

Query: 245 TTAYDEYHCLKSLNALTSYHNDQIKQAIEVLKKENLHTVIVYGDYYNAFLWILRRASMLG 304
           T  YD+  CL  LN     HN+Q+++AI  L+KE     IVYGDYYNAF ++LR      
Sbjct: 242 TKDYDDNGCLTHLNEFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNAFQYVLRSER--- 301

Query: 305 FDNGSLQKSCCGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKYIADWLI 364
           FD     KSCCG GG YN+D KR  G  GV VC NP K ISWDGVHLTQKAY++++ +L 
Sbjct: 302 FDKSVALKSCCGTGGAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKAYRFMSKFLN 321

Query: 365 LNISPKLNCT 374
             I  ++ CT
Sbjct: 362 NQILSQIKCT 321

BLAST of CSPI02G04350 vs. ExPASy Swiss-Prot
Match: Q9SHP6 (GDSL esterase/lipase At1g28610 OS=Arabidopsis thaliana OX=3702 GN=At1g28610 PE=2 SV=2)

HSP 1 Score: 267.7 bits (683), Expect = 2.0e-70
Identity = 151/366 (41.26%), Postives = 215/366 (58.74%), Query Frame = 0

Query: 5   SSSSSITSFVFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLI-----RENP 64
           +S  S+ SF    LF+ ++ S +      +  N + I   GDSI+DTGNL+        P
Sbjct: 2   ASLDSLVSFFLSTLFVTIVSSQT------QCRNLESIISFGDSITDTGNLVGLSDRNHLP 61

Query: 65  NTPFSHLPYGQTFFSTPTGRCSNGLLIIDYFALDARLPLVNP-YLNKDALTRHGINFAVA 124
            T F  LPYG+TFF  PTGR  NG +IID+ A    LP V P Y +K+     G+NFAVA
Sbjct: 62  VTAF--LPYGETFFHHPTGRSCNGRIIIDFIAEFLGLPHVPPFYGSKNGNFEKGVNFAVA 121

Query: 125 GSTALSSELLSKKKISSLLTNSSLDLQLDWMFSHFNSICYDQKDCDEKLKNALFLVGEIG 184
           G+TAL + +L K+ I    +N SL +QL        ++C    DC + + NA  ++GEIG
Sbjct: 122 GATALETSILEKRGIYYPHSNISLGIQLKTFKESLPNLCGSPTDCRDMIGNAFIIMGEIG 181

Query: 185 ANDYNYALLQGKTIEEVKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTV 244
            ND+N+A    KT  EVKE+VP V+  I +A+  ++  G    +V GNFP+GC    LT+
Sbjct: 182 GNDFNFAFFVNKT-SEVKELVPLVITKISSAIVELVDMGGRTFLVPGNFPLGCSATYLTL 241

Query: 245 FQTNNTTAYDEY-HCLKSLNALTSYHNDQIKQAIEVLKKENLHTVIVYGDYYNAFLWILR 304
           +QT+N   YD    CL  LN  + Y+N++++  +  L K   H  I+YGDY+NA L + +
Sbjct: 242 YQTSNKEEYDPLTGCLTWLNDFSEYYNEKLQAELNRLSKLYPHVNIIYGDYFNALLRLYQ 301

Query: 305 RASMLGFDNGSLQKSCCGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKY 364
             S  GF +  L  +CCG+GG YNF L + CG+ GV  C +P K ++WDGVH+T+ AYK+
Sbjct: 302 EPSKFGFMDRPL-PACCGLGGPYNFTLSKKCGSVGVKYCSDPSKYVNWDGVHMTEAAYKW 357

BLAST of CSPI02G04350 vs. ExPASy TrEMBL
Match: A0A5A7TYC8 (Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00460 PE=3 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 9.9e-198
Identity = 342/374 (91.44%), Postives = 361/374 (96.52%), Query Frame = 0

Query: 2   ASSSSSSSITSFVFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLIRENPNT 61
           +SSSSSSSITSFVFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLIRENPNT
Sbjct: 5   SSSSSSSSITSFVFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLIRENPNT 64

Query: 62  PFSHLPYGQTFF-STPTGRCSNGLLIIDYFALDARLPLVNPYLNKDALTRHGINFAVAGS 121
           PFSHLPYGQTFF STPTGRCSNGLLIIDYFALDA+LPLVNPYLNKDALTRHG+NFAVAGS
Sbjct: 65  PFSHLPYGQTFFNSTPTGRCSNGLLIIDYFALDAKLPLVNPYLNKDALTRHGVNFAVAGS 124

Query: 122 TALSSELLSKKKISSLLTNSSLDLQLDWMFSHFNSICYDQKDCDEKLKNALFLVGEIGAN 181
           TALSS+LLS+KKISS LTNSSLD+QLDWMFSHFNSIC+DQKDC+EKL NALFLVGEIGAN
Sbjct: 125 TALSSQLLSEKKISSPLTNSSLDIQLDWMFSHFNSICFDQKDCNEKLMNALFLVGEIGAN 184

Query: 182 DYNYALLQGKTIEEVKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTVFQ 241
           DYNYALLQGKTI+EVKEMVPEVVQ +KNAVERVISYGATRVVVSGNFPIGCFPI LTVFQ
Sbjct: 185 DYNYALLQGKTIQEVKEMVPEVVQTVKNAVERVISYGATRVVVSGNFPIGCFPIFLTVFQ 244

Query: 242 TNNTTAYDEYHCLKSLNALTSYHNDQIKQAIEVLKKENLHTVIVYGDYYNAFLWILRRAS 301
           TN+TTAYDE+HCLKSLNALTSYHNDQI+QAIEVL KEN  TVIVYGDYYNAFLWILR AS
Sbjct: 245 TNDTTAYDEFHCLKSLNALTSYHNDQIQQAIEVLNKENSRTVIVYGDYYNAFLWILRHAS 304

Query: 302 MLGFDNGSLQKSCCGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKYIAD 361
            LGFDNGSLQKSCCGIGGDYNF+LK++CG+ GVGVCPN DKVISWDGV LTQKAYK+IAD
Sbjct: 305 TLGFDNGSLQKSCCGIGGDYNFELKKSCGSQGVGVCPNADKVISWDGVQLTQKAYKFIAD 364

Query: 362 WLILNISPKLNCTL 375
           WLILNI+PKLNCTL
Sbjct: 365 WLILNITPKLNCTL 378

BLAST of CSPI02G04350 vs. ExPASy TrEMBL
Match: A0A1S3CP03 (acetylajmalan esterase-like OS=Cucumis melo OX=3656 GN=LOC103503119 PE=3 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 7.3e-193
Identity = 331/362 (91.44%), Postives = 349/362 (96.41%), Query Frame = 0

Query: 14  VFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLIRENPNTPFSHLPYGQTFF 73
           VFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLIRENPNTPFSHLPYGQTFF
Sbjct: 1   VFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLIRENPNTPFSHLPYGQTFF 60

Query: 74  -STPTGRCSNGLLIIDYFALDARLPLVNPYLNKDALTRHGINFAVAGSTALSSELLSKKK 133
            STPTGRCSNGLLIIDYFALDA+LPLVNPYLNKDALTRHG+NFAVAGSTALSS+LLS+KK
Sbjct: 61  NSTPTGRCSNGLLIIDYFALDAKLPLVNPYLNKDALTRHGVNFAVAGSTALSSQLLSEKK 120

Query: 134 ISSLLTNSSLDLQLDWMFSHFNSICYDQKDCDEKLKNALFLVGEIGANDYNYALLQGKTI 193
           ISS LTNSSLD+QLDWMFSHFNSIC+DQKDC+EKL NALFLVGEIGANDYNYALLQGKTI
Sbjct: 121 ISSPLTNSSLDIQLDWMFSHFNSICFDQKDCNEKLMNALFLVGEIGANDYNYALLQGKTI 180

Query: 194 EEVKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTVFQTNNTTAYDEYHC 253
           +EVKEMVPEVVQ +KNAVERVISYGATRVVVSGNFPIGCFPI LTVFQTN+TTAYDE+HC
Sbjct: 181 QEVKEMVPEVVQTVKNAVERVISYGATRVVVSGNFPIGCFPIFLTVFQTNDTTAYDEFHC 240

Query: 254 LKSLNALTSYHNDQIKQAIEVLKKENLHTVIVYGDYYNAFLWILRRASMLGFDNGSLQKS 313
           LKSLNALTSYHNDQI+QAIEVL KEN  TVIVYGDYYNAFLWILR AS LGFDNGSLQKS
Sbjct: 241 LKSLNALTSYHNDQIQQAIEVLNKENSRTVIVYGDYYNAFLWILRHASTLGFDNGSLQKS 300

Query: 314 CCGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKYIADWLILNISPKLNC 373
           CCGIGGDYNF+LK++CG+ GVGVCPN DKVISWDGV LTQKAYK+IADWLILNI+PKLNC
Sbjct: 301 CCGIGGDYNFELKKSCGSQGVGVCPNADKVISWDGVQLTQKAYKFIADWLILNITPKLNC 360

Query: 374 TL 375
           TL
Sbjct: 361 TL 362

BLAST of CSPI02G04350 vs. ExPASy TrEMBL
Match: A0A6J1KFS8 (acetylajmalan esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111493497 PE=3 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.4e-164
Identity = 292/372 (78.49%), Postives = 317/372 (85.22%), Query Frame = 0

Query: 1   MASSSSSSSITSFVFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLIRENPN 60
           MASS+ S      V   + + +L   SS A SLKAC FD IYQ+GDSISDTGNLI +NPN
Sbjct: 1   MASSTKSPLSIVLVVLIVLVSVLGCSSSKAPSLKACMFDAIYQVGDSISDTGNLILQNPN 60

Query: 61  TPFSHLPYGQTFFSTPTGRCSNGLLIIDYFALDARLPLVNPYLNKDALTRHGINFAVAGS 120
           TPFSHLPYG+TFF+  TGRCSNGLL+IDYFALDA LPLVNPYLNKDALTRHG+NFAVAGS
Sbjct: 61  TPFSHLPYGETFFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTRHGVNFAVAGS 120

Query: 121 TALSSELLSKKKISSLLTNSSLDLQLDWMFSHFNSICYDQKDCDEKLKNALFLVGEIGAN 180
           TALSSE LS+KKISSL+TNSSLD QLDWMFSHFNSICYDQ DC EKLKNALFLVGEIG N
Sbjct: 121 TALSSEALSQKKISSLVTNSSLDQQLDWMFSHFNSICYDQIDCIEKLKNALFLVGEIGGN 180

Query: 181 DYNYALLQGKTIEEVKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTVFQ 240
           DYNYALLQGKT +EVK+MV EVVQ IKNAVERVISYGATRVVV GNFPIGC PI LT FQ
Sbjct: 181 DYNYALLQGKTNQEVKDMVSEVVQTIKNAVERVISYGATRVVVPGNFPIGCLPIYLTRFQ 240

Query: 241 TNNTTAYDEYHCLKSLNALTSYHNDQIKQAIEVLKKENLHTVIVYGDYYNAFLWILRRAS 300
           TN+T AYDE HCLK LN L  YHNDQIKQ IEVLKKEN HTVIVYGDYYNA LWILRRA 
Sbjct: 241 TNDTIAYDELHCLKDLNDLAIYHNDQIKQVIEVLKKENPHTVIVYGDYYNALLWILRRAF 300

Query: 301 MLGFDNGSLQKSCCGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKYIAD 360
           +LGFD  SLQKSCCGIGG+YNFDL +TCG +GV VC NPD  +SWDGVHLTQKAYK++AD
Sbjct: 301 ILGFDKTSLQKSCCGIGGEYNFDLMKTCGVSGVSVCSNPDDRVSWDGVHLTQKAYKFMAD 360

Query: 361 WLILNISPKLNC 373
           WLI +I P+L+C
Sbjct: 361 WLIHDILPQLHC 372

BLAST of CSPI02G04350 vs. ExPASy TrEMBL
Match: A0A6J1G595 (acetylajmalan esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111450820 PE=3 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 4.6e-163
Identity = 287/372 (77.15%), Postives = 314/372 (84.41%), Query Frame = 0

Query: 1   MASSSSSSSITSFVFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLIRENPN 60
           MA+S+ S      V   + + +    SS A SLKAC FD IYQ+GDSISDTGNLI +NPN
Sbjct: 1   MAASTKSPLSIVLVVLLVLVSVHGCSSSKAPSLKACMFDAIYQVGDSISDTGNLILQNPN 60

Query: 61  TPFSHLPYGQTFFSTPTGRCSNGLLIIDYFALDARLPLVNPYLNKDALTRHGINFAVAGS 120
           TPFSHLPYG+TFF+  TGRCSNGLLIIDYFA+DA LPL+NPYLNKDALTRHG+NFAVAGS
Sbjct: 61  TPFSHLPYGETFFNKSTGRCSNGLLIIDYFAMDAGLPLLNPYLNKDALTRHGVNFAVAGS 120

Query: 121 TALSSELLSKKKISSLLTNSSLDLQLDWMFSHFNSICYDQKDCDEKLKNALFLVGEIGAN 180
           TALSSE LS+KKISSL+TNSSLD QLDWMFSHFNSICY+Q+DC EKLKNALFLVGEIG N
Sbjct: 121 TALSSEALSQKKISSLVTNSSLDQQLDWMFSHFNSICYNQRDCIEKLKNALFLVGEIGGN 180

Query: 181 DYNYALLQGKTIEEVKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTVFQ 240
           DYNYALLQGKTI EVK+MVPEVVQ IK AVERVISYGAT VVV GNFPIGC PI LT FQ
Sbjct: 181 DYNYALLQGKTIPEVKDMVPEVVQTIKKAVERVISYGATHVVVPGNFPIGCLPIYLTRFQ 240

Query: 241 TNNTTAYDEYHCLKSLNALTSYHNDQIKQAIEVLKKENLHTVIVYGDYYNAFLWILRRAS 300
           TN+ TAYDE HCLK LN L  YHNDQIKQ IEVLKKEN HTVIVYGDYYNA LWILR A 
Sbjct: 241 TNDATAYDELHCLKDLNGLAIYHNDQIKQVIEVLKKENPHTVIVYGDYYNALLWILRSAF 300

Query: 301 MLGFDNGSLQKSCCGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKYIAD 360
            LGFD  SLQKSCCGIGGDYNFDL +TCG  GV VC NPD+ +SWDG+HLTQKAYK++AD
Sbjct: 301 TLGFDKTSLQKSCCGIGGDYNFDLMKTCGVGGVSVCSNPDERVSWDGIHLTQKAYKFMAD 360

Query: 361 WLILNISPKLNC 373
           WLI +I P+L+C
Sbjct: 361 WLIQDILPQLHC 372

BLAST of CSPI02G04350 vs. ExPASy TrEMBL
Match: A0A6J1G4V8 (GDSL esterase/lipase At5g03980-like OS=Cucurbita moschata OX=3662 GN=LOC111450822 PE=3 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.6e-160
Identity = 279/372 (75.00%), Postives = 316/372 (84.95%), Query Frame = 0

Query: 1   MASSSSSSSITSFVFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLIRENPN 60
           MA S+ S S        + L +L+ PSS A  LK C FD IYQLGDSISDTGNLI +NPN
Sbjct: 1   MAPSTKSPSPIVLAVLIVLLSVLVCPSSTAHLLKTCMFDTIYQLGDSISDTGNLILQNPN 60

Query: 61  TPFSHLPYGQTFFSTPTGRCSNGLLIIDYFALDARLPLVNPYLNKDALTRHGINFAVAGS 120
           TPFSHLPYGQ+FF+  TGRCSNGLL+IDYFALDA LPLVNPYLNKDA TRHG+NFAVAGS
Sbjct: 61  TPFSHLPYGQSFFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDASTRHGVNFAVAGS 120

Query: 121 TALSSELLSKKKISSLLTNSSLDLQLDWMFSHFNSICYDQKDCDEKLKNALFLVGEIGAN 180
           TALSS LLS+K+I S +TN+SLD QLDWMFSHFNSICY+Q+DC+EKL+NALFLVGEIG N
Sbjct: 121 TALSSHLLSQKQILSPVTNTSLDQQLDWMFSHFNSICYNQRDCNEKLRNALFLVGEIGGN 180

Query: 181 DYNYALLQGKTIEEVKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTVFQ 240
           DYNYAL QGKTI+EVK+MVP+VVQ IKNAVE+VISYGATRVVV GNFPIGC PI LT FQ
Sbjct: 181 DYNYALFQGKTIQEVKDMVPQVVQTIKNAVEKVISYGATRVVVPGNFPIGCLPIYLTGFQ 240

Query: 241 TNNTTAYDEYHCLKSLNALTSYHNDQIKQAIEVLKKENLHTVIVYGDYYNAFLWILRRAS 300
           TN+TTAYDE HCLK LN L +YHNDQIKQAI+VLK+EN H+VI+YGDYYNA LWILRRA 
Sbjct: 241 TNDTTAYDELHCLKDLNDLATYHNDQIKQAIQVLKRENPHSVIIYGDYYNALLWILRRAF 300

Query: 301 MLGFDNGSLQKSCCGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKYIAD 360
           +LGFD  SLQKSCCGIGGDYNF L + CG  GV VC NPD+ +SWDG+HLTQKAYK++A 
Sbjct: 301 ILGFDEASLQKSCCGIGGDYNFSLVKMCGVGGVSVCSNPDERVSWDGIHLTQKAYKFMAY 360

Query: 361 WLILNISPKLNC 373
           WLI +I P+L+C
Sbjct: 361 WLIHDIFPQLHC 372

BLAST of CSPI02G04350 vs. NCBI nr
Match: XP_031737210.1 (acetylajmalan esterase-like [Cucumis sativus])

HSP 1 Score: 738.8 bits (1906), Expect = 2.3e-209
Identity = 368/377 (97.61%), Postives = 369/377 (97.88%), Query Frame = 0

Query: 2   ASSSSSSSITSFVFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLIRENPNT 61
           +SSSSSSSITSFVFPFLFLFLLLSPSSNA SLKACNFDGIYQLGDSISDTGNLIRENPNT
Sbjct: 3   SSSSSSSSITSFVFPFLFLFLLLSPSSNADSLKACNFDGIYQLGDSISDTGNLIRENPNT 62

Query: 62  PFSHLPYGQTFF-STPTGRCSNGLLIIDYFALDARLPLVNPYLNKDALTRHGINFAVAGS 121
           PFSHLPYGQTFF STPTGRCSNGLLIIDYFALDARLPLVNPYLNKDALTRHGINFAVAGS
Sbjct: 63  PFSHLPYGQTFFNSTPTGRCSNGLLIIDYFALDARLPLVNPYLNKDALTRHGINFAVAGS 122

Query: 122 TALSSELLSKKKISSLLTNSSLDLQLDWMFSHFNSICYDQKDCDEKLKNALFLVGEIGAN 181
           TALSSELLSKKKISSLLTNSSLDLQLDWMFSHFNSICYDQKDCDEKLKNALFLVGEIGAN
Sbjct: 123 TALSSELLSKKKISSLLTNSSLDLQLDWMFSHFNSICYDQKDCDEKLKNALFLVGEIGAN 182

Query: 182 DYNYALLQGKTIEEVKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTVFQ 241
           DYNYALLQGKTIEEVKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTVFQ
Sbjct: 183 DYNYALLQGKTIEEVKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTVFQ 242

Query: 242 TNNTT---AYDEYHCLKSLNALTSYHNDQIKQAIEVLKKENLHTVIVYGDYYNAFLWILR 301
           TNNTT    YDEYHCLKSLNAL SYHNDQIKQ IEVLKKENLHTVIVYGDYYNAFLWILR
Sbjct: 243 TNNTTDYDEYDEYHCLKSLNALASYHNDQIKQVIEVLKKENLHTVIVYGDYYNAFLWILR 302

Query: 302 RASMLGFDNGSLQKSCCGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKY 361
           RASMLGFDNGSLQKSCCGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKY
Sbjct: 303 RASMLGFDNGSLQKSCCGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKY 362

Query: 362 IADWLILNISPKLNCTL 375
           IADWLILNISPKLNCTL
Sbjct: 363 IADWLILNISPKLNCTL 379

BLAST of CSPI02G04350 vs. NCBI nr
Match: KAA0048034.1 (acetylajmalan esterase-like [Cucumis melo var. makuwa] >TYK13784.1 acetylajmalan esterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 699.1 bits (1803), Expect = 2.0e-197
Identity = 342/374 (91.44%), Postives = 361/374 (96.52%), Query Frame = 0

Query: 2   ASSSSSSSITSFVFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLIRENPNT 61
           +SSSSSSSITSFVFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLIRENPNT
Sbjct: 5   SSSSSSSSITSFVFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLIRENPNT 64

Query: 62  PFSHLPYGQTFF-STPTGRCSNGLLIIDYFALDARLPLVNPYLNKDALTRHGINFAVAGS 121
           PFSHLPYGQTFF STPTGRCSNGLLIIDYFALDA+LPLVNPYLNKDALTRHG+NFAVAGS
Sbjct: 65  PFSHLPYGQTFFNSTPTGRCSNGLLIIDYFALDAKLPLVNPYLNKDALTRHGVNFAVAGS 124

Query: 122 TALSSELLSKKKISSLLTNSSLDLQLDWMFSHFNSICYDQKDCDEKLKNALFLVGEIGAN 181
           TALSS+LLS+KKISS LTNSSLD+QLDWMFSHFNSIC+DQKDC+EKL NALFLVGEIGAN
Sbjct: 125 TALSSQLLSEKKISSPLTNSSLDIQLDWMFSHFNSICFDQKDCNEKLMNALFLVGEIGAN 184

Query: 182 DYNYALLQGKTIEEVKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTVFQ 241
           DYNYALLQGKTI+EVKEMVPEVVQ +KNAVERVISYGATRVVVSGNFPIGCFPI LTVFQ
Sbjct: 185 DYNYALLQGKTIQEVKEMVPEVVQTVKNAVERVISYGATRVVVSGNFPIGCFPIFLTVFQ 244

Query: 242 TNNTTAYDEYHCLKSLNALTSYHNDQIKQAIEVLKKENLHTVIVYGDYYNAFLWILRRAS 301
           TN+TTAYDE+HCLKSLNALTSYHNDQI+QAIEVL KEN  TVIVYGDYYNAFLWILR AS
Sbjct: 245 TNDTTAYDEFHCLKSLNALTSYHNDQIQQAIEVLNKENSRTVIVYGDYYNAFLWILRHAS 304

Query: 302 MLGFDNGSLQKSCCGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKYIAD 361
            LGFDNGSLQKSCCGIGGDYNF+LK++CG+ GVGVCPN DKVISWDGV LTQKAYK+IAD
Sbjct: 305 TLGFDNGSLQKSCCGIGGDYNFELKKSCGSQGVGVCPNADKVISWDGVQLTQKAYKFIAD 364

Query: 362 WLILNISPKLNCTL 375
           WLILNI+PKLNCTL
Sbjct: 365 WLILNITPKLNCTL 378

BLAST of CSPI02G04350 vs. NCBI nr
Match: KGN60996.2 (hypothetical protein Csa_021157 [Cucumis sativus])

HSP 1 Score: 688.7 bits (1776), Expect = 2.8e-194
Identity = 337/345 (97.68%), Postives = 338/345 (97.97%), Query Frame = 0

Query: 34  KACNFDGIYQLGDSISDTGNLIRENPNTPFSHLPYGQTFF-STPTGRCSNGLLIIDYFAL 93
           +ACNFDGIYQLGDSISDTGNLIRENPNTPFSHLPYGQTFF STPTGRCSNGLLIIDYFAL
Sbjct: 434 EACNFDGIYQLGDSISDTGNLIRENPNTPFSHLPYGQTFFNSTPTGRCSNGLLIIDYFAL 493

Query: 94  DARLPLVNPYLNKDALTRHGINFAVAGSTALSSELLSKKKISSLLTNSSLDLQLDWMFSH 153
           DARLPLVNPYLNKDALTRHGINFAVAGSTALSSELLSKKKISSLLTNSSLDLQLDWMFSH
Sbjct: 494 DARLPLVNPYLNKDALTRHGINFAVAGSTALSSELLSKKKISSLLTNSSLDLQLDWMFSH 553

Query: 154 FNSICYDQKDCDEKLKNALFLVGEIGANDYNYALLQGKTIEEVKEMVPEVVQAIKNAVER 213
           FNSICYDQKDCDEKLKNALFLVGEIGANDYNYALLQGKTIEEVKEMVPEVVQAIKNAVER
Sbjct: 554 FNSICYDQKDCDEKLKNALFLVGEIGANDYNYALLQGKTIEEVKEMVPEVVQAIKNAVER 613

Query: 214 VISYGATRVVVSGNFPIGCFPISLTVFQTNNTT---AYDEYHCLKSLNALTSYHNDQIKQ 273
           VISYGATRVVVSGNFPIGCFPISLTVFQTNNTT    YDEYHCLKSLNAL SYHNDQIKQ
Sbjct: 614 VISYGATRVVVSGNFPIGCFPISLTVFQTNNTTDYDEYDEYHCLKSLNALASYHNDQIKQ 673

Query: 274 AIEVLKKENLHTVIVYGDYYNAFLWILRRASMLGFDNGSLQKSCCGIGGDYNFDLKRTCG 333
            IEVLKKENLHTVIVYGDYYNAFLWILRRASMLGFDNGSLQKSCCGIGGDYNFDLKRTCG
Sbjct: 674 VIEVLKKENLHTVIVYGDYYNAFLWILRRASMLGFDNGSLQKSCCGIGGDYNFDLKRTCG 733

Query: 334 NNGVGVCPNPDKVISWDGVHLTQKAYKYIADWLILNISPKLNCTL 375
           NNGVGVCPNPDKVISWDGVHLTQKAYKYIADWLILNISPKLNCTL
Sbjct: 734 NNGVGVCPNPDKVISWDGVHLTQKAYKYIADWLILNISPKLNCTL 778

BLAST of CSPI02G04350 vs. NCBI nr
Match: XP_008465497.2 (PREDICTED: acetylajmalan esterase-like, partial [Cucumis melo])

HSP 1 Score: 682.9 bits (1761), Expect = 1.5e-192
Identity = 331/362 (91.44%), Postives = 349/362 (96.41%), Query Frame = 0

Query: 14  VFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLIRENPNTPFSHLPYGQTFF 73
           VFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLIRENPNTPFSHLPYGQTFF
Sbjct: 1   VFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLIRENPNTPFSHLPYGQTFF 60

Query: 74  -STPTGRCSNGLLIIDYFALDARLPLVNPYLNKDALTRHGINFAVAGSTALSSELLSKKK 133
            STPTGRCSNGLLIIDYFALDA+LPLVNPYLNKDALTRHG+NFAVAGSTALSS+LLS+KK
Sbjct: 61  NSTPTGRCSNGLLIIDYFALDAKLPLVNPYLNKDALTRHGVNFAVAGSTALSSQLLSEKK 120

Query: 134 ISSLLTNSSLDLQLDWMFSHFNSICYDQKDCDEKLKNALFLVGEIGANDYNYALLQGKTI 193
           ISS LTNSSLD+QLDWMFSHFNSIC+DQKDC+EKL NALFLVGEIGANDYNYALLQGKTI
Sbjct: 121 ISSPLTNSSLDIQLDWMFSHFNSICFDQKDCNEKLMNALFLVGEIGANDYNYALLQGKTI 180

Query: 194 EEVKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTVFQTNNTTAYDEYHC 253
           +EVKEMVPEVVQ +KNAVERVISYGATRVVVSGNFPIGCFPI LTVFQTN+TTAYDE+HC
Sbjct: 181 QEVKEMVPEVVQTVKNAVERVISYGATRVVVSGNFPIGCFPIFLTVFQTNDTTAYDEFHC 240

Query: 254 LKSLNALTSYHNDQIKQAIEVLKKENLHTVIVYGDYYNAFLWILRRASMLGFDNGSLQKS 313
           LKSLNALTSYHNDQI+QAIEVL KEN  TVIVYGDYYNAFLWILR AS LGFDNGSLQKS
Sbjct: 241 LKSLNALTSYHNDQIQQAIEVLNKENSRTVIVYGDYYNAFLWILRHASTLGFDNGSLQKS 300

Query: 314 CCGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKYIADWLILNISPKLNC 373
           CCGIGGDYNF+LK++CG+ GVGVCPN DKVISWDGV LTQKAYK+IADWLILNI+PKLNC
Sbjct: 301 CCGIGGDYNFELKKSCGSQGVGVCPNADKVISWDGVQLTQKAYKFIADWLILNITPKLNC 360

Query: 374 TL 375
           TL
Sbjct: 361 TL 362

BLAST of CSPI02G04350 vs. NCBI nr
Match: XP_022998969.1 (acetylajmalan esterase-like [Cucurbita maxima])

HSP 1 Score: 588.2 bits (1515), Expect = 5.1e-164
Identity = 292/372 (78.49%), Postives = 317/372 (85.22%), Query Frame = 0

Query: 1   MASSSSSSSITSFVFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLIRENPN 60
           MASS+ S      V   + + +L   SS A SLKAC FD IYQ+GDSISDTGNLI +NPN
Sbjct: 1   MASSTKSPLSIVLVVLIVLVSVLGCSSSKAPSLKACMFDAIYQVGDSISDTGNLILQNPN 60

Query: 61  TPFSHLPYGQTFFSTPTGRCSNGLLIIDYFALDARLPLVNPYLNKDALTRHGINFAVAGS 120
           TPFSHLPYG+TFF+  TGRCSNGLL+IDYFALDA LPLVNPYLNKDALTRHG+NFAVAGS
Sbjct: 61  TPFSHLPYGETFFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTRHGVNFAVAGS 120

Query: 121 TALSSELLSKKKISSLLTNSSLDLQLDWMFSHFNSICYDQKDCDEKLKNALFLVGEIGAN 180
           TALSSE LS+KKISSL+TNSSLD QLDWMFSHFNSICYDQ DC EKLKNALFLVGEIG N
Sbjct: 121 TALSSEALSQKKISSLVTNSSLDQQLDWMFSHFNSICYDQIDCIEKLKNALFLVGEIGGN 180

Query: 181 DYNYALLQGKTIEEVKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTVFQ 240
           DYNYALLQGKT +EVK+MV EVVQ IKNAVERVISYGATRVVV GNFPIGC PI LT FQ
Sbjct: 181 DYNYALLQGKTNQEVKDMVSEVVQTIKNAVERVISYGATRVVVPGNFPIGCLPIYLTRFQ 240

Query: 241 TNNTTAYDEYHCLKSLNALTSYHNDQIKQAIEVLKKENLHTVIVYGDYYNAFLWILRRAS 300
           TN+T AYDE HCLK LN L  YHNDQIKQ IEVLKKEN HTVIVYGDYYNA LWILRRA 
Sbjct: 241 TNDTIAYDELHCLKDLNDLAIYHNDQIKQVIEVLKKENPHTVIVYGDYYNALLWILRRAF 300

Query: 301 MLGFDNGSLQKSCCGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKYIAD 360
           +LGFD  SLQKSCCGIGG+YNFDL +TCG +GV VC NPD  +SWDGVHLTQKAYK++AD
Sbjct: 301 ILGFDKTSLQKSCCGIGGEYNFDLMKTCGVSGVSVCSNPDDRVSWDGVHLTQKAYKFMAD 360

Query: 361 WLILNISPKLNC 373
           WLI +I P+L+C
Sbjct: 361 WLIHDILPQLHC 372

BLAST of CSPI02G04350 vs. TAIR 10
Match: AT1G28590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 273.5 bits (698), Expect = 2.6e-73
Identity = 147/350 (42.00%), Postives = 215/350 (61.43%), Query Frame = 0

Query: 19  FLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLI-RENPN-TPFSHL-PYGQTFFST 78
           F+   L  +S  +  +  NF  I   GDSI+DTGNL+   +PN  P S   PYG+TFF  
Sbjct: 15  FILSTLLVTSVNSQTQCRNFKSIISFGDSIADTGNLLGLSDPNDLPASAFPPYGETFFHH 74

Query: 79  PTGRCSNGLLIIDYFALDARLPLVNPYLN-KDALTRHGINFAVAGSTALSSELLSKKKIS 138
           PTGR S+G LIID+ A     PLV P+   ++A  + G+NFAVAG+TAL    L ++ I 
Sbjct: 75  PTGRYSDGRLIIDFIAEFLGFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFLEERGIH 134

Query: 139 SLLTNSSLDLQLDWMFSHFNSICYDQKDCDEKLKNALFLVGEIGANDYNYALLQGKTIEE 198
           S +TN SL +QL        ++C    DC + ++NAL L+GEIG NDYN+AL Q K ++E
Sbjct: 135 STITNVSLSVQLRSFTESLPNLCGSPSDCRDMIENALILMGEIGGNDYNFALFQRKPVKE 194

Query: 199 VKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTVFQTNNTTAYDEY-HCL 258
           V+E+VP V+  I +A+  ++  G    +V GNFPIG     LT+++T+N   YD    CL
Sbjct: 195 VEELVPFVIATISSAITELVCMGGRTFLVPGNFPIGYSASYLTLYKTSNKEEYDPLTGCL 254

Query: 259 KSLNALTSYHNDQIKQAIEVLKKENLHTVIVYGDYYNAFLWILRRASMLGFDNGSLQKSC 318
           K LN  + Y+N Q+++ +  L+K   H  I+Y DYYNA L + +  +  GF N  L  +C
Sbjct: 255 KWLNDFSEYYNKQLQEELNGLRKLYPHVNIIYADYYNALLRLFQEPAKFGFMNRPL-PAC 314

Query: 319 CGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKYIADWLI 364
           CG+GG YNF+  R CG+ GV  C +P + +++DG+H+T+ AY+ I++ L+
Sbjct: 315 CGVGGSYNFNFSRRCGSVGVEYCDDPSQYVNYDGIHMTEAAYRLISEGLL 363

BLAST of CSPI02G04350 vs. TAIR 10
Match: AT1G31550.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 273.1 bits (697), Expect = 3.4e-73
Identity = 154/350 (44.00%), Postives = 210/350 (60.00%), Query Frame = 0

Query: 20  LFLLLSPSSNAASLKAC-NFDGIYQLGDSISDTGNL--IRENPNTPFSHL-PYGQTFFST 79
           LFL     S  +S   C NF+ I   GDSI+DTGNL  + ++ N P S   PYG+TFF  
Sbjct: 15  LFLSTLFVSIVSSESQCRNFESIISFGDSIADTGNLLGLSDHNNLPMSAFPPYGETFFHH 74

Query: 80  PTGRCSNGLLIIDYFALDARLPLVNPYL-NKDALTRHGINFAVAGSTALSSELLSKKKIS 139
           PTGR S+G LIID+ A    LP V PY  + +     G+NFAVA +TAL S  L +K   
Sbjct: 75  PTGRFSDGRLIIDFIAEFLGLPYVPPYFGSTNGNFEKGVNFAVASATALESSFLEEKGY- 134

Query: 140 SLLTNSSLDLQLDWMFSHFNSICYDQKDCDEKLKNALFLVGEIGANDYNYALLQGKTIEE 199
               N SL +QL        ++C    DC + + NAL L+GEIGANDYN+   Q + ++E
Sbjct: 135 HCPHNFSLGVQLKIFKQSLPNLCGLPSDCRDMIGNALILMGEIGANDYNFPFFQLRPLDE 194

Query: 200 VKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTVFQTNNTTAYDEY-HCL 259
           VKE+VP V+  I +A+  +I  G    +V G FP+GC    LT+ QT+N   YD    CL
Sbjct: 195 VKELVPLVISTISSAITELIGMGGRTFLVPGGFPLGCSVAFLTLHQTSNMEEYDPLTGCL 254

Query: 260 KSLNALTSYHNDQIKQAIEVLKKENLHTVIVYGDYYNAFLWILRRASMLGFDNGSLQKSC 319
           K LN    YH++Q+++ +  L+K N H  I+Y DYYNA L + R  S  GF N  L  +C
Sbjct: 255 KWLNKFGEYHSEQLQEELNRLRKLNPHVNIIYADYYNASLRLGREPSKYGFINRHL-SAC 314

Query: 320 CGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKYIADWLI 364
           CG+GG YNF+L R+CG+ GV  C +P K ++WDG+H+T+ A+K +AD L+
Sbjct: 315 CGVGGPYNFNLSRSCGSVGVEACSDPSKYVAWDGLHMTEAAHKSMADGLV 362

BLAST of CSPI02G04350 vs. TAIR 10
Match: AT5G03980.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 270.4 bits (690), Expect = 2.2e-72
Identity = 161/370 (43.51%), Postives = 205/370 (55.41%), Query Frame = 0

Query: 5   SSSSSITSFVFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLIRENPNTPFS 64
           S++ +++  VF    LF+ L  SS+      C  + IYQ GDSISDTGNLIR  P     
Sbjct: 2   STTKALSLLVF---ILFVSLVHSSD-----QCPINSIYQFGDSISDTGNLIRNGP----- 61

Query: 65  HLPYGQTFFSTPTGRCSNGLLIIDYFALDARLPLVNPYLNKDALTRHGINFAVAGSTALS 124
                    S+PT                   P   P    +      +NF V+GSTAL+
Sbjct: 62  --------ASSPT-------------------PKPLPQREHNVF----VNFGVSGSTALN 121

Query: 125 SELLSKKKISSLLTNSSLDLQLDWMFSHFNSICY-DQKDCDEKLKNALFLVGEIGANDYN 184
           S   S++ +    TN+ L +QL W   H  S C+    DC   LK++LF+VGEIG NDYN
Sbjct: 122 SSFFSERNLHVPATNTPLSMQLAWFKGHLRSTCHGSSSDC---LKHSLFMVGEIGGNDYN 181

Query: 185 YALLQGKTIEEVKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTVFQTNN 244
           Y   QGK +EE++  +P VV AI  A   VI  GA  VVV GNFP+GCFPI LT F   +
Sbjct: 182 YGFFQGKPMEEIRSYIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKD 241

Query: 245 TTAYDEYHCLKSLNALTSYHNDQIKQAIEVLKKENLHTVIVYGDYYNAFLWILRRASMLG 304
           T  YD+  CL  LN     HN+Q+++AI  L+KE     IVYGDYYNAF ++LR      
Sbjct: 242 TKDYDDNGCLTHLNEFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNAFQYVLRSER--- 301

Query: 305 FDNGSLQKSCCGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKYIADWLI 364
           FD     KSCCG GG YN+D KR  G  GV VC NP K ISWDGVHLTQKAY++++ +L 
Sbjct: 302 FDKSVALKSCCGTGGAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKAYRFMSKFLN 321

Query: 365 LNISPKLNCT 374
             I  ++ CT
Sbjct: 362 NQILSQIKCT 321

BLAST of CSPI02G04350 vs. TAIR 10
Match: AT1G28610.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 267.7 bits (683), Expect = 1.4e-71
Identity = 151/366 (41.26%), Postives = 215/366 (58.74%), Query Frame = 0

Query: 5   SSSSSITSFVFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLI-----RENP 64
           +S  S+ SF    LF+ ++ S +      +  N + I   GDSI+DTGNL+        P
Sbjct: 2   ASLDSLVSFFLSTLFVTIVSSQT------QCRNLESIISFGDSITDTGNLVGLSDRNHLP 61

Query: 65  NTPFSHLPYGQTFFSTPTGRCSNGLLIIDYFALDARLPLVNP-YLNKDALTRHGINFAVA 124
            T F  LPYG+TFF  PTGR  NG +IID+ A    LP V P Y +K+     G+NFAVA
Sbjct: 62  VTAF--LPYGETFFHHPTGRSCNGRIIIDFIAEFLGLPHVPPFYGSKNGNFEKGVNFAVA 121

Query: 125 GSTALSSELLSKKKISSLLTNSSLDLQLDWMFSHFNSICYDQKDCDEKLKNALFLVGEIG 184
           G+TAL + +L K+ I    +N SL +QL        ++C    DC + + NA  ++GEIG
Sbjct: 122 GATALETSILEKRGIYYPHSNISLGIQLKTFKESLPNLCGSPTDCRDMIGNAFIIMGEIG 181

Query: 185 ANDYNYALLQGKTIEEVKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTV 244
            ND+N+A    KT  EVKE+VP V+  I +A+  ++  G    +V GNFP+GC    LT+
Sbjct: 182 GNDFNFAFFVNKT-SEVKELVPLVITKISSAIVELVDMGGRTFLVPGNFPLGCSATYLTL 241

Query: 245 FQTNNTTAYDEY-HCLKSLNALTSYHNDQIKQAIEVLKKENLHTVIVYGDYYNAFLWILR 304
           +QT+N   YD    CL  LN  + Y+N++++  +  L K   H  I+YGDY+NA L + +
Sbjct: 242 YQTSNKEEYDPLTGCLTWLNDFSEYYNEKLQAELNRLSKLYPHVNIIYGDYFNALLRLYQ 301

Query: 305 RASMLGFDNGSLQKSCCGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKY 364
             S  GF +  L  +CCG+GG YNF L + CG+ GV  C +P K ++WDGVH+T+ AYK+
Sbjct: 302 EPSKFGFMDRPL-PACCGLGGPYNFTLSKKCGSVGVKYCSDPSKYVNWDGVHMTEAAYKW 357

BLAST of CSPI02G04350 vs. TAIR 10
Match: AT1G28600.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 266.9 bits (681), Expect = 2.4e-71
Identity = 150/366 (40.98%), Postives = 212/366 (57.92%), Query Frame = 0

Query: 5   SSSSSITSFVFPFLFLFLLLSPSSNAASLKACNFDGIYQLGDSISDTGNLI-----RENP 64
           +S  S+  F+F  LF+ ++ S +         NF  I   GDSI+DTGNL+      + P
Sbjct: 2   ASLDSLVIFLFSTLFVTIVSSET------PCPNFKSIISFGDSIADTGNLVGLSDRNQLP 61

Query: 65  NTPFSHLPYGQTFFSTPTGRCSNGLLIIDYFALDARLPLVNPYL-NKDALTRHGINFAVA 124
            T F   PYG+TFF  PTGR  +G +I+D+ A    LP V PY  +K+     G+NFAVA
Sbjct: 62  VTAFP--PYGETFFHHPTGRSCDGRIIMDFIAEFVGLPYVPPYFGSKNRNFDKGVNFAVA 121

Query: 125 GSTALSSELLSKKKISSLLTNSSLDLQLDWMFSHFNSICYDQKDCDEKLKNALFLVGEIG 184
           G+TAL S  L K+ I    TN SL +QL        ++C    DC + + NAL L+GEIG
Sbjct: 122 GATALKSSFLKKRGIQP-HTNVSLGVQLKSFKKSLPNLCGSPSDCRDMIGNALILMGEIG 181

Query: 185 ANDYNYALLQGKTIEEVKEMVPEVVQAIKNAVERVISYGATRVVVSGNFPIGCFPISLTV 244
            NDYN+     K ++EV+E+VP V+ +I + +  +I  G    +V G FPIGC  + LT+
Sbjct: 182 GNDYNFPFFNRKPVKEVEELVPFVIASISSTITELIGMGGKTFLVPGEFPIGCSVVYLTL 241

Query: 245 FQTNNTTAYD-EYHCLKSLNALTSYHNDQIKQAIEVLKKENLHTVIVYGDYYNAFLWILR 304
           ++T+N   YD    CLK LN    YH++++K  +  L+K   H  I+Y DYYN+ L I +
Sbjct: 242 YKTSNKDEYDPSTGCLKWLNKFGEYHSEKLKVELNRLRKLYPHVNIIYADYYNSLLRIFK 301

Query: 305 RASMLGFDNGSLQKSCCGIGGDYNFDLKRTCGNNGVGVCPNPDKVISWDGVHLTQKAYKY 364
             +  GF       +CCGIGG YNF+  R CG+ GV  C +P K + WDGVH+T+ AYK+
Sbjct: 302 EPAKFGFMERPF-PACCGIGGPYNFNFTRKCGSVGVKSCKDPSKYVGWDGVHMTEAAYKW 357