CSPI02G01860 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G01860
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPhytocyanin domain-containing protein
LocationChr2: 1291355 .. 1293036 (+)
RNA-Seq ExpressionCSPI02G01860
SyntenyCSPI02G01860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCAGCACAATGAGCTTCGTTTCCTGTCTCATTATTGCTTTAGCATTGGCAGCTGCCATGGCTACAACTTCAACTGCTTCAACCACCTATAAGGTTGGAGATGATCAGGGCTGGAAGGTTCCCAAGGACGACCCTGCCCATTACATGGCATGGCCTGTCAATAAGACATTCACTGTCGGTGACAAACTCGGTAAATAACCCTTTTATTCGAACGCTTTACTAATGTCGTCTCTGTCTTTGCTCCTAACAAGTGTTTGCTTACAACTCTTTAGAACTCAACGACATAGTTGTACAACTAATTGAACGAATCATAATTGCGTCGTATTTGACTTTGAAAATTAAAACTCATTATTTACTTAGTATAGGTTGCATTTCTAACTTAAAACTCATGAAGGGAACCTGGATATTACAAAAAGGTTTGTTTTATGAGTTAAATTTGCACATGTAAACATATCCATGAGCATCTAAGTTCAAACTTTAAAGGGATTGTTTGAGGCACTAAGTTCGTTATTATAGTTTGTGGATTATAATGGTTTGTGTTTGGTATGAGTTGTAACACCTCATCCTACCCAAGACAAGTTGATAATCCAAACACTTCCTAACTTATTTAAAACATGGAGTTGTGAGCTTCAAGACGCTGTTAAGTTTGGAGAGTTAAAAACCATTCAACCCACATCGTAAGGAGTTAATAAGACATCATATTGATGTTTTTTGTTCCTAAGACTCTAGAACTCCTTTAGCCAAACAAAGAGCAGGGAACTAGTACAAGTACTTGAATGGTGTTGAATTTCAATCAAATCTGTAAGAAAAATAGAAGAGTCAAGAGAAGTAAGACAGCAGATCAATGTCACATTTCAACTAACAGGTAAAACTATTGAGATCAAAGAGTACAGAACTAAAGATGACCCATTTCCAATTACCCTAGTGAGCTTTCTCTTCTACAATTTGGCTCAAACTTAACTGTGTTTCCTTTTCACAACATTATTTTACACTTAATTTTATTTGTTTATTTTAAACATTTGACTTTCATATGAGATTTACTTCATAATTATTTTTTGTATAAATAAGATCTTCTCTCTTTTGTTTCCAGAATTCACATGGACGGGCACACATAATGTGGCTGAGGTAACAAAGGAAGAGTATACAAGATGTGTTGAGGTAAAAACAGTTCATGAGTTTAGCCCTGTGACAATCTCTCTTGATACTCCTGGCCCCAAATACTTTATCTGTGCCGTCGTTCCCCACTGCTCGTTTGGGCAGAGGTTAACTATCATTGTCGAACCTGATAGCTCGACAACGCCACCAGCACCAGAACCAACACCAAGCTCTGCTCCCTCCTCACTCCTGGCAAATTCTTTGTATGCAGCTATGCTCACCATAGTTAGCATGTTCTTCACTCGTCTGTAAGAAGACTTCAAGATCTTTTGGCTCATCTTCATTATGTATTTTATGTGCTGTAACAATGTATTGAGAAGTAGGAAATCCTGGCTAGTGGTATTTGCCAGCCTGTTCAGTTTCATTTACCATTATTGAAATAAAAAATTAACTTTATTTGTAAAGGAATTTACAGAAGGTTTCATATCAGCAAGTTCCCACACATGACATGTTTCTCTTTCCAATAATACCTCTATGATGTTTACATCCATCTTTGGCTCTGGGTTGGGGGAAGCTAAAGAAAA

mRNA sequence

ATGGGCAGCACAATGAGCTTCGTTTCCTGTCTCATTATTGCTTTAGCATTGGCAGCTGCCATGGCTACAACTTCAACTGCTTCAACCACCTATAAGGTTGGAGATGATCAGGGCTGGAAGGTTCCCAAGGACGACCCTGCCCATTACATGGCATGGCCTGTCAATAAGACATTCACTGTCGGTGACAAACTCGAATTCACATGGACGGGCACACATAATGTGGCTGAGGTAACAAAGGAAGAGTATACAAGATGTGTTGAGGTAAAAACAGTTCATGAGTTTAGCCCTGTGACAATCTCTCTTGATACTCCTGGCCCCAAATACTTTATCTGTGCCGTCGTTCCCCACTGCTCGTTTGGGCAGAGGTTAACTATCATTGTCGAACCTGATAGCTCGACAACGCCACCAGCACCAGAACCAACACCAAGCTCTGCTCCCTCCTCACTCCTGGCAAATTCTTTGTATGCAGCTATGCTCACCATAGTTAGCATGTTCTTCACTCGTCTGTAAGAAGACTTCAAGATCTTTTGGCTCATCTTCATTATGTATTTTATGTGCTGTAACAATGTATTGAGAAGTAGGAAATCCTGGCTAGTGGTATTTGCCAGCCTGTTCAGTTTCATTTACCATTATTGAAATAAAAAATTAACTTTATTTGTAAAGGAATTTACAGAAGGTTTCATATCAGCAAGTTCCCACACATGACATGTTTCTCTTTCCAATAATACCTCTATGATGTTTACATCCATCTTTGGCTCTGGGTTGGGGGAAGCTAAAGAAAA

Coding sequence (CDS)

ATGGGCAGCACAATGAGCTTCGTTTCCTGTCTCATTATTGCTTTAGCATTGGCAGCTGCCATGGCTACAACTTCAACTGCTTCAACCACCTATAAGGTTGGAGATGATCAGGGCTGGAAGGTTCCCAAGGACGACCCTGCCCATTACATGGCATGGCCTGTCAATAAGACATTCACTGTCGGTGACAAACTCGAATTCACATGGACGGGCACACATAATGTGGCTGAGGTAACAAAGGAAGAGTATACAAGATGTGTTGAGGTAAAAACAGTTCATGAGTTTAGCCCTGTGACAATCTCTCTTGATACTCCTGGCCCCAAATACTTTATCTGTGCCGTCGTTCCCCACTGCTCGTTTGGGCAGAGGTTAACTATCATTGTCGAACCTGATAGCTCGACAACGCCACCAGCACCAGAACCAACACCAAGCTCTGCTCCCTCCTCACTCCTGGCAAATTCTTTGTATGCAGCTATGCTCACCATAGTTAGCATGTTCTTCACTCGTCTGTAA

Protein sequence

MGSTMSFVSCLIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTVGDKLEFTWTGTHNVAEVTKEEYTRCVEVKTVHEFSPVTISLDTPGPKYFICAVVPHCSFGQRLTIIVEPDSSTTPPAPEPTPSSAPSSLLANSLYAAMLTIVSMFFTRL*
Homology
BLAST of CSPI02G01860 vs. ExPASy Swiss-Prot
Match: Q07488 (Blue copper protein OS=Arabidopsis thaliana OX=3702 GN=BCB PE=2 SV=2)

HSP 1 Score: 89.7 bits (221), Expect = 3.4e-17
Identity = 61/155 (39.35%), Postives = 79/155 (50.97%), Query Frame = 0

Query: 1   MGSTMSFVSCLIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTV 60
           M      V+ L++  A     A        Y VGDD  W  P  DP  Y  W   KTF V
Sbjct: 1   MAGVFKTVTFLVLVFAAVVVFA------EDYDVGDDTEWTRPM-DPEFYTTWATGKTFRV 60

Query: 61  GDKLEFTW-TGTHNVAEVTKEEYTRCVEVKTVHEFS--PVTISLDTPGPKYFICAVVPHC 120
           GD+LEF +  G H+VA V++  +  C + K +   +  PV I L+T GP+YFIC V  HC
Sbjct: 61  GDELEFDFAAGRHDVAVVSEAAFENCEKEKPISHMTVPPVKIMLNTTGPQYFICTVGDHC 120

Query: 121 SFGQRLTIIVEPDSST---TPPA-PEPTPSSAPSS 149
            FGQ+L+I V    +T   TP A   P P S PS+
Sbjct: 121 RFGQKLSITVVAAGATGGATPGAGATPAPGSTPST 148

BLAST of CSPI02G01860 vs. ExPASy Swiss-Prot
Match: P42849 (Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 3.4e-17
Identity = 46/100 (46.00%), Postives = 60/100 (60.00%), Query Frame = 0

Query: 31  YKVGDDQGWKVPKDDPAHYMAWPVNKTFTVGDKLEFTW-TGTHNVAEVTKEEYTRCVEVK 90
           Y VG D  WK P  DP  Y+ W   KTF VGD+LEF +  G H+VA VTK+ +  C +  
Sbjct: 3   YDVGGDMEWKRP-SDPKFYITWATGKTFRVGDELEFDFAAGMHDVAVVTKDAFDNCKKEN 62

Query: 91  TVHEFS--PVTISLDTPGPKYFICAVVPHCSFGQRLTIIV 128
            +   +  PV I L+T GP+Y+IC V  HC  GQ+L+I V
Sbjct: 63  PISHMTTPPVKIMLNTTGPQYYICTVGDHCRVGQKLSINV 101

BLAST of CSPI02G01860 vs. ExPASy Swiss-Prot
Match: P29602 (Cucumber peeling cupredoxin OS=Cucumis sativus OX=3659 PE=1 SV=3)

HSP 1 Score: 83.6 bits (205), Expect = 2.4e-15
Identity = 51/124 (41.13%), Postives = 69/124 (55.65%), Query Frame = 0

Query: 28  STTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTVGDKLEFTW-TGTHNVAEV-TKEEYTRC 87
           ST + VGD+ GW VP   P  Y  W   KTF VGD L+F +    HNV E+ TK+ +  C
Sbjct: 2   STVHIVGDNTGWSVP-SSPNFYSQWAAGKTFRVGDSLQFNFPANAHNVHEMETKQSFDAC 61

Query: 88  VEVKT---VHEFSPVTISLDTPGPKYFICAVVPHCSFGQRLTI-IVEPDSSTTPPAPEPT 146
             V +   V   SPV   LD  G  YF+C V  HCS GQ+L+I +V  +++ + P P  +
Sbjct: 62  NFVNSDNDVERTSPVIERLDELGMHYFVCTVGTHCSNGQKLSINVVAANATVSMPPPSSS 121

BLAST of CSPI02G01860 vs. ExPASy Swiss-Prot
Match: O82081 (Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 4.5e-14
Identity = 51/148 (34.46%), Postives = 76/148 (51.35%), Query Frame = 0

Query: 11  LIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTVGDKLEFTWTG 70
           ++I +++ A      T +T + +G   GW V     A    W   +TF VGD L F++  
Sbjct: 6   MLIIISVLATTLIGLTVATDHTIGGPSGWTV----GASLRTWAAGQTFAVGDNLVFSYPA 65

Query: 71  T-HNVAEVTKEEYTRCVEVKTVHEFS--PVTISLDTPGPKYFICAVVPHCSFGQRLTIIV 130
             H+V EVTK E+  C  VK +  F+     + L TPG +YFIC +  HCS G +L + V
Sbjct: 66  AFHDVVEVTKPEFDSCQAVKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVNV 125

Query: 131 EPDSSTTPPAPEPTP-----SSAPSSLL 151
            P ++  P AP P       + +PSS+L
Sbjct: 126 VPTATVAPTAPLPNTVPSLNAPSPSSVL 149

BLAST of CSPI02G01860 vs. ExPASy Swiss-Prot
Match: Q41001 (Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.3e-13
Identity = 53/144 (36.81%), Postives = 72/144 (50.00%), Query Frame = 0

Query: 5   MSFVSCLIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTVGDKL 64
           M+F + L++   LA       + +T Y VGD  GW +  D    Y  W  +KTF VGD L
Sbjct: 1   MAFSNALVLCFLLAIINMALPSLATVYTVGDTSGWVIGGD----YSTWASDKTFAVGDSL 60

Query: 65  EFTW-TGTHNVAEVTKEEYTRCVEVKTVHEFS--PVTISLDTPGPKYFICAVVPHCSFGQ 124
            F +  G H V EV + +Y  C    ++   S    TI L   G  YFIC V  H + G 
Sbjct: 61  VFNYGAGAHTVDEVKESDYKSCTSGNSISTDSTGATTIPLKKAGKHYFICGVPGHSTGGM 120

Query: 125 RLTIIVEPDSSTTPPAPEPTPSSA 146
           +L+I V+  SS +  AP  TPSS+
Sbjct: 121 KLSIKVKA-SSGSSAAPSATPSSS 139

BLAST of CSPI02G01860 vs. ExPASy TrEMBL
Match: A0A0A0LI34 (Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G006890 PE=4 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.7e-88
Identity = 168/169 (99.41%), Postives = 169/169 (100.00%), Query Frame = 0

Query: 1   MGSTMSFVSCLIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTV 60
           MGSTMSFVSCLIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTV
Sbjct: 1   MGSTMSFVSCLIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTV 60

Query: 61  GDKLEFTWTGTHNVAEVTKEEYTRCVEVKTVHEFSPVTISLDTPGPKYFICAVVPHCSFG 120
           GDKLEFTWTGTHNVAEVTKEEYTRCVEVKTVHEFSPVTISLDTPGPKYFICAVVPHCSFG
Sbjct: 61  GDKLEFTWTGTHNVAEVTKEEYTRCVEVKTVHEFSPVTISLDTPGPKYFICAVVPHCSFG 120

Query: 121 QRLTIIVEPDSSTTPPAPEPTPSSAPSSLLANSLYAAMLTIVSMFFTRL 170
           QRLTI+VEPDSSTTPPAPEPTPSSAPSSLLANSLYAAMLTIVSMFFTRL
Sbjct: 121 QRLTIVVEPDSSTTPPAPEPTPSSAPSSLLANSLYAAMLTIVSMFFTRL 169

BLAST of CSPI02G01860 vs. ExPASy TrEMBL
Match: A0A1S3C4X2 (umecyanin-like OS=Cucumis melo OX=3656 GN=LOC103496698 PE=4 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 1.0e-80
Identity = 155/169 (91.72%), Postives = 162/169 (95.86%), Query Frame = 0

Query: 1   MGSTMSFVSCLIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTV 60
           MGSTM FV CLIIALAL AAMATTSTASTTYKVGDDQGWKVPKDDPA YMAWPVNKTFTV
Sbjct: 1   MGSTMRFVWCLIIALAL-AAMATTSTASTTYKVGDDQGWKVPKDDPALYMAWPVNKTFTV 60

Query: 61  GDKLEFTWTGTHNVAEVTKEEYTRCVEVKTVHEFSPVTISLDTPGPKYFICAVVPHCSFG 120
           GDKLEFTWTGTHNVAEVTKE+YT+C+EVKTVHEFSPVTISLDTPGPKYFICA+VPHCSFG
Sbjct: 61  GDKLEFTWTGTHNVAEVTKEDYTKCIEVKTVHEFSPVTISLDTPGPKYFICAIVPHCSFG 120

Query: 121 QRLTIIVEPDSSTTPPAPEPTPSSAPSSLLANSLYAAMLTIVSMFFTRL 170
           +RLTI+VEPD+STTPPAP P PSSAPSSLLANSLYAAMLTI SMFFTRL
Sbjct: 121 ERLTIVVEPDNSTTPPAPAPAPSSAPSSLLANSLYAAMLTIASMFFTRL 168

BLAST of CSPI02G01860 vs. ExPASy TrEMBL
Match: A0A6J1CNF9 (umecyanin-like OS=Momordica charantia OX=3673 GN=LOC111013077 PE=4 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 5.0e-32
Identity = 84/165 (50.91%), Postives = 107/165 (64.85%), Query Frame = 0

Query: 12  IIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTVGDKLEFTWTGT 71
           ++ALA +AAMA T  A+ T++VG D+GW VP+ + + Y  W  +KTF VGDKLEF WTG 
Sbjct: 40  VVALA-SAAMAATGIAAITHEVGGDKGWAVPQ-EMSFYTTWASDKTFNVGDKLEFKWTGL 99

Query: 72  HNVAEVTKEEYTRCVEVKTVHEFSPVTISLDTPGPKYFICAVVPHCSFGQRLTIIVEPDS 131
           H+V EVTK+ +  C    T +  SPV I L+T G KYFIC V PHCS GQ+L++ V   +
Sbjct: 100 HSVLEVTKDGFENCTSSGTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTVGSKN 159

Query: 132 STTPPAP-------EPTPSSAPSSLLANSLYAAMLTIVSMFFTRL 170
           S  P AP        P PSSAP+SLLAN+L AA   I S+ FT L
Sbjct: 160 SAPPAAPTTPATPAAPAPSSAPASLLANALSAATFAIGSILFTCL 202

BLAST of CSPI02G01860 vs. ExPASy TrEMBL
Match: A0A2I4F530 (umecyanin-like OS=Juglans regia OX=51240 GN=LOC108995609 PE=4 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 2.8e-22
Identity = 71/172 (41.28%), Postives = 100/172 (58.14%), Query Frame = 0

Query: 1   MGSTMS-FVSCLIIALALA---AAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNK 60
           MGS M+  + CLI+ +AL     A A T T++  YKVGD  GW VP  + ++Y  W   +
Sbjct: 1   MGSRMNMIIGCLIVVVALVHINGASAATVTSAGDYKVGDSLGWDVP-PNTSYYADWASTQ 60

Query: 61  TFTVGDKLEFTWTGTHNVAEVTKEEYTRCVEVKTVHEFSPVTISLDTPGPKYFICAVVPH 120
           TF +GDK  F WTGTHN+AEV+K +Y  C +V + +  SPVT +  + G  YFIC V  H
Sbjct: 61  TFFLGDKFFFNWTGTHNLAEVSKADYDNCTKVSS-YIGSPVTYTPPSTGSYYFICTVNDH 120

Query: 121 CSFGQRLTIIVE-PDSSTTPPAPEPTPSSAPSSLLANSLYAAMLTIVSMFFT 168
           C  GQ+L + +  P+ +T   +P   P S+ SSL   +L+A + T    F T
Sbjct: 121 CERGQKLALTISAPNFTTVSESPAEPPISSASSLTIGALFAVLSTAAISFQT 170

BLAST of CSPI02G01860 vs. ExPASy TrEMBL
Match: A0A5A7VDE4 (Umecyanin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00790 PE=4 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 4.7e-22
Identity = 60/64 (93.75%), Postives = 60/64 (93.75%), Query Frame = 0

Query: 1  MGSTMSFVSCLIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTV 60
          MGSTM FV CLIIALAL AAMATTSTASTTYKVGDDQGWKVPKDDPA YMAWPVNKTFTV
Sbjct: 1  MGSTMRFVWCLIIALAL-AAMATTSTASTTYKVGDDQGWKVPKDDPALYMAWPVNKTFTV 60

Query: 61 GDKL 65
          GDKL
Sbjct: 61 GDKL 63

BLAST of CSPI02G01860 vs. NCBI nr
Match: XP_004139411.1 (umecyanin [Cucumis sativus])

HSP 1 Score: 335.1 bits (858), Expect = 3.5e-88
Identity = 168/169 (99.41%), Postives = 169/169 (100.00%), Query Frame = 0

Query: 1   MGSTMSFVSCLIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTV 60
           MGSTMSFVSCLIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTV
Sbjct: 1   MGSTMSFVSCLIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTV 60

Query: 61  GDKLEFTWTGTHNVAEVTKEEYTRCVEVKTVHEFSPVTISLDTPGPKYFICAVVPHCSFG 120
           GDKLEFTWTGTHNVAEVTKEEYTRCVEVKTVHEFSPVTISLDTPGPKYFICAVVPHCSFG
Sbjct: 61  GDKLEFTWTGTHNVAEVTKEEYTRCVEVKTVHEFSPVTISLDTPGPKYFICAVVPHCSFG 120

Query: 121 QRLTIIVEPDSSTTPPAPEPTPSSAPSSLLANSLYAAMLTIVSMFFTRL 170
           QRLTI+VEPDSSTTPPAPEPTPSSAPSSLLANSLYAAMLTIVSMFFTRL
Sbjct: 121 QRLTIVVEPDSSTTPPAPEPTPSSAPSSLLANSLYAAMLTIVSMFFTRL 169

BLAST of CSPI02G01860 vs. NCBI nr
Match: XP_008456887.1 (PREDICTED: umecyanin-like [Cucumis melo])

HSP 1 Score: 309.3 bits (791), Expect = 2.1e-80
Identity = 155/169 (91.72%), Postives = 162/169 (95.86%), Query Frame = 0

Query: 1   MGSTMSFVSCLIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTV 60
           MGSTM FV CLIIALAL AAMATTSTASTTYKVGDDQGWKVPKDDPA YMAWPVNKTFTV
Sbjct: 1   MGSTMRFVWCLIIALAL-AAMATTSTASTTYKVGDDQGWKVPKDDPALYMAWPVNKTFTV 60

Query: 61  GDKLEFTWTGTHNVAEVTKEEYTRCVEVKTVHEFSPVTISLDTPGPKYFICAVVPHCSFG 120
           GDKLEFTWTGTHNVAEVTKE+YT+C+EVKTVHEFSPVTISLDTPGPKYFICA+VPHCSFG
Sbjct: 61  GDKLEFTWTGTHNVAEVTKEDYTKCIEVKTVHEFSPVTISLDTPGPKYFICAIVPHCSFG 120

Query: 121 QRLTIIVEPDSSTTPPAPEPTPSSAPSSLLANSLYAAMLTIVSMFFTRL 170
           +RLTI+VEPD+STTPPAP P PSSAPSSLLANSLYAAMLTI SMFFTRL
Sbjct: 121 ERLTIVVEPDNSTTPPAPAPAPSSAPSSLLANSLYAAMLTIASMFFTRL 168

BLAST of CSPI02G01860 vs. NCBI nr
Match: KGN60711.2 (hypothetical protein Csa_019338 [Cucumis sativus])

HSP 1 Score: 301.2 bits (770), Expect = 5.6e-78
Identity = 154/172 (89.53%), Postives = 155/172 (90.12%), Query Frame = 0

Query: 15  LALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTVGDKL---------- 74
           LALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTVGDKL          
Sbjct: 214 LALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTVGDKLGCISNLKLMK 273

Query: 75  -------EFTWTGTHNVAEVTKEEYTRCVEVKTVHEFSPVTISLDTPGPKYFICAVVPHC 134
                  EFTWTGTHNVAEVTKEEYTRCVEVKTVHEFSPVTISLDTPGPKYFICAVVPHC
Sbjct: 274 GTRILQKEFTWTGTHNVAEVTKEEYTRCVEVKTVHEFSPVTISLDTPGPKYFICAVVPHC 333

Query: 135 SFGQRLTIIVEPDSSTTPPAPEPTPSSAPSSLLANSLYAAMLTIVSMFFTRL 170
           SFGQRLTI+VEPDSSTTPPAPEPTPSSAPSSLLANSLYAAMLTIVSMFFTRL
Sbjct: 334 SFGQRLTIVVEPDSSTTPPAPEPTPSSAPSSLLANSLYAAMLTIVSMFFTRL 385

BLAST of CSPI02G01860 vs. NCBI nr
Match: XP_038890066.1 (umecyanin-like [Benincasa hispida])

HSP 1 Score: 227.6 bits (579), Expect = 7.9e-56
Identity = 124/170 (72.94%), Postives = 137/170 (80.59%), Query Frame = 0

Query: 1   MGSTMSFVSCLIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTV 60
           MGSTMSFV C+ IALAL A MATT+TAST +KVGDDQGW+ PKDDP+ Y  W  NKTF V
Sbjct: 1   MGSTMSFVWCM-IALAL-AVMATTATASTIHKVGDDQGWRFPKDDPSFYTRWAANKTFIV 60

Query: 61  GDKLEFTWTGTHNVAEVTKEEYTRCVEVKT-VHEFSPVTISLDTPGPKYFICAVVPHCSF 120
           GDKLEF WTGTHNV EV K+EY  C E +T +HE SPVTISL T G KYFICAVVPHCSF
Sbjct: 61  GDKLEFIWTGTHNVVEVRKDEYGNCTEGETPLHELSPVTISLGTAGSKYFICAVVPHCSF 120

Query: 121 GQRLTIIVEPDSSTTPPAPEPTPSSAPSSLLANSLYAAMLTIVSMFFTRL 170
           GQ+L++IVE D ST PPA    PSSAP+SLLAN+LYAAMLTI SMFFT L
Sbjct: 121 GQKLSVIVESDISTMPPA--SAPSSAPTSLLANALYAAMLTITSMFFTCL 166

BLAST of CSPI02G01860 vs. NCBI nr
Match: XP_022143139.1 (umecyanin-like [Momordica charantia])

HSP 1 Score: 147.5 bits (371), Expect = 1.0e-31
Identity = 84/165 (50.91%), Postives = 107/165 (64.85%), Query Frame = 0

Query: 12  IIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTVGDKLEFTWTGT 71
           ++ALA +AAMA T  A+ T++VG D+GW VP+ + + Y  W  +KTF VGDKLEF WTG 
Sbjct: 40  VVALA-SAAMAATGIAAITHEVGGDKGWAVPQ-EMSFYTTWASDKTFNVGDKLEFKWTGL 99

Query: 72  HNVAEVTKEEYTRCVEVKTVHEFSPVTISLDTPGPKYFICAVVPHCSFGQRLTIIVEPDS 131
           H+V EVTK+ +  C    T +  SPV I L+T G KYFIC V PHCS GQ+L++ V   +
Sbjct: 100 HSVLEVTKDGFENCTSSGTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKLSVTVGSKN 159

Query: 132 STTPPAP-------EPTPSSAPSSLLANSLYAAMLTIVSMFFTRL 170
           S  P AP        P PSSAP+SLLAN+L AA   I S+ FT L
Sbjct: 160 SAPPAAPTTPATPAAPAPSSAPASLLANALSAATFAIGSILFTCL 202

BLAST of CSPI02G01860 vs. TAIR 10
Match: AT5G20230.1 (blue-copper-binding protein )

HSP 1 Score: 89.7 bits (221), Expect = 2.4e-18
Identity = 61/155 (39.35%), Postives = 79/155 (50.97%), Query Frame = 0

Query: 1   MGSTMSFVSCLIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTV 60
           M      V+ L++  A     A        Y VGDD  W  P  DP  Y  W   KTF V
Sbjct: 1   MAGVFKTVTFLVLVFAAVVVFA------EDYDVGDDTEWTRPM-DPEFYTTWATGKTFRV 60

Query: 61  GDKLEFTW-TGTHNVAEVTKEEYTRCVEVKTVHEFS--PVTISLDTPGPKYFICAVVPHC 120
           GD+LEF +  G H+VA V++  +  C + K +   +  PV I L+T GP+YFIC V  HC
Sbjct: 61  GDELEFDFAAGRHDVAVVSEAAFENCEKEKPISHMTVPPVKIMLNTTGPQYFICTVGDHC 120

Query: 121 SFGQRLTIIVEPDSST---TPPA-PEPTPSSAPSS 149
            FGQ+L+I V    +T   TP A   P P S PS+
Sbjct: 121 RFGQKLSITVVAAGATGGATPGAGATPAPGSTPST 148

BLAST of CSPI02G01860 vs. TAIR 10
Match: AT2G32300.1 (uclacyanin 1 )

HSP 1 Score: 79.3 bits (194), Expect = 3.2e-15
Identity = 51/148 (34.46%), Postives = 76/148 (51.35%), Query Frame = 0

Query: 11  LIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTVGDKLEFTWTG 70
           ++I +++ A      T +T + +G   GW V     A    W   +TF VGD L F++  
Sbjct: 6   MLIIISVLATTLIGLTVATDHTIGGPSGWTV----GASLRTWAAGQTFAVGDNLVFSYPA 65

Query: 71  T-HNVAEVTKEEYTRCVEVKTVHEFS--PVTISLDTPGPKYFICAVVPHCSFGQRLTIIV 130
             H+V EVTK E+  C  VK +  F+     + L TPG +YFIC +  HCS G +L + V
Sbjct: 66  AFHDVVEVTKPEFDSCQAVKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVNV 125

Query: 131 EPDSSTTPPAPEPTP-----SSAPSSLL 151
            P ++  P AP P       + +PSS+L
Sbjct: 126 VPTATVAPTAPLPNTVPSLNAPSPSSVL 149

BLAST of CSPI02G01860 vs. TAIR 10
Match: AT3G60270.1 (Cupredoxin superfamily protein )

HSP 1 Score: 79.3 bits (194), Expect = 3.2e-15
Identity = 54/157 (34.39%), Postives = 79/157 (50.32%), Query Frame = 0

Query: 1   MGSTMSFVSCLIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTV 60
           MGST +    L++ L     +A  +  + T++VGD+ GW +       Y +W   KTF V
Sbjct: 1   MGSTAATALLLLLLL-----VAVPAVFAVTFQVGDNDGWTI----GVEYTSWVSEKTFRV 60

Query: 61  GDKLEFTWTGTHNVAEVTKEEYTRCVEVKTVHEFS--PVTISLDTPGPKYFICAVVPHCS 120
           GD LEF +  +H+VA V K +Y  C   +    FS     I L   G  +F+C    HCS
Sbjct: 61  GDTLEFKYGPSHSVAVVNKADYDGCETSRPTQSFSDGDTKIDLTKVGAIHFLCLTPGHCS 120

Query: 121 FGQRLTIIV------EPDSSTTPPAPEPT-PSSAPSS 149
            G +L + V      EP  S + P+P P+ PS +PS+
Sbjct: 121 LGMKLAVQVLAAVSLEPPPSPSAPSPSPSAPSPSPSA 148

BLAST of CSPI02G01860 vs. TAIR 10
Match: AT5G07475.1 (Cupredoxin superfamily protein )

HSP 1 Score: 77.8 bits (190), Expect = 9.4e-15
Identity = 45/128 (35.16%), Postives = 66/128 (51.56%), Query Frame = 0

Query: 28  STTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTVGDKLEFTWTGTHNVAEVTKEEYTRCVE 87
           +TTY VGD  GW +  D      +W   K F+ GD L F ++ TH+V EV K+ Y  C  
Sbjct: 27  ATTYFVGDSSGWDISSD----LESWTSGKRFSPGDVLMFQYSSTHSVYEVAKDNYQNCNT 86

Query: 88  VKTVHEFS--PVTISLDTPGPKYFICAVVPHCSFGQRLTIIVEPDSSTTPPAPEPTPSSA 147
              +  F+    T++L  PG ++F+C    HC  G RL + VE +  +   AP  +P +A
Sbjct: 87  TDAIRTFTNGNTTVALSKPGNRFFVCGNRLHCFAGMRLLVNVEGNGPS--QAPVGSPQAA 146

Query: 148 PSSLLANS 154
            S +L  S
Sbjct: 147 TSGILQPS 148

BLAST of CSPI02G01860 vs. TAIR 10
Match: AT3G60280.1 (uclacyanin 3 )

HSP 1 Score: 76.3 bits (186), Expect = 2.7e-14
Identity = 53/155 (34.19%), Postives = 73/155 (47.10%), Query Frame = 0

Query: 1   MGSTMSFVSCLIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTV 60
           MGST++    L +A       A  +  + T+KVGD  GW    D    Y  W   KTF V
Sbjct: 1   MGSTVAAALLLFLA-------AVPAVFAATFKVGDISGWTSNLD----YTVWLTGKTFRV 60

Query: 61  GDKLEFTWTGTHNVAEVTKEEYTRCVEVKTVHEFS--PVTISLDTPGPKYFICAVVPHCS 120
           GD LEF +  +H+V+ V K  Y  C        F+     I L T G  +F+C    HC 
Sbjct: 61  GDTLEFVYGLSHSVSVVDKAGYDNCDSSGATQNFADGDTKIDLTTVGTMHFLCPTFGHCK 120

Query: 121 FGQRLTIIV-----EPDSSTTPPAPEPTPSSAPSS 149
            G +L + V      P + ++PP+   TPSS PS+
Sbjct: 121 NGMKLAVPVLAAAPSPSTPSSPPSTPSTPSSPPST 144

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q074883.4e-1739.35Blue copper protein OS=Arabidopsis thaliana OX=3702 GN=BCB PE=2 SV=2[more]
P428493.4e-1746.00Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1[more]
P296022.4e-1541.13Cucumber peeling cupredoxin OS=Cucumis sativus OX=3659 PE=1 SV=3[more]
O820814.5e-1434.46Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1[more]
Q410012.3e-1336.81Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LI341.7e-8899.41Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G006890... [more]
A0A1S3C4X21.0e-8091.72umecyanin-like OS=Cucumis melo OX=3656 GN=LOC103496698 PE=4 SV=1[more]
A0A6J1CNF95.0e-3250.91umecyanin-like OS=Momordica charantia OX=3673 GN=LOC111013077 PE=4 SV=1[more]
A0A2I4F5302.8e-2241.28umecyanin-like OS=Juglans regia OX=51240 GN=LOC108995609 PE=4 SV=1[more]
A0A5A7VDE44.7e-2293.75Umecyanin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00790... [more]
Match NameE-valueIdentityDescription
XP_004139411.13.5e-8899.41umecyanin [Cucumis sativus][more]
XP_008456887.12.1e-8091.72PREDICTED: umecyanin-like [Cucumis melo][more]
KGN60711.25.6e-7889.53hypothetical protein Csa_019338 [Cucumis sativus][more]
XP_038890066.17.9e-5672.94umecyanin-like [Benincasa hispida][more]
XP_022143139.11.0e-3150.91umecyanin-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G20230.12.4e-1839.35blue-copper-binding protein [more]
AT2G32300.13.2e-1534.46uclacyanin 1 [more]
AT3G60270.13.2e-1534.39Cupredoxin superfamily protein [more]
AT5G07475.19.4e-1535.16Cupredoxin superfamily protein [more]
AT3G60280.12.7e-1434.19uclacyanin 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003245Phytocyanin domainPFAMPF02298Cu_bind_likecoord: 39..121
e-value: 1.1E-19
score: 70.2
IPR003245Phytocyanin domainPROSITEPS51485PHYTOCYANINcoord: 29..129
score: 29.844677
IPR008972CupredoxinGENE3D2.60.40.420coord: 28..137
e-value: 3.5E-28
score: 99.9
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 28..127
IPR039391PhytocyaninPANTHERPTHR33021BLUE COPPER PROTEINcoord: 7..142
NoneNo IPR availablePANTHERPTHR33021:SF360OS08G0482600 PROTEINcoord: 7..142

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G01860.1CSPI02G01860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0022900 electron transport chain
biological_process GO:0042795 snRNA transcription by RNA polymerase II
biological_process GO:0042796 snRNA transcription by RNA polymerase III
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0019185 snRNA-activating protein complex
molecular_function GO:0009055 electron transfer activity
molecular_function GO:0043565 sequence-specific DNA binding