CSPI02G00840 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G00840
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	cytochrome B5-like protein
Location	Chr2: 509324 .. 511736 (-)
RNA-Seq Expression	CSPI02G00840
Synteny	CSPI02G00840

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGGAATTTTGAGAGTGATTCTGGTAAGCATTTCCCCGGCTAATTCATTTTGGCATTCAGTTCACCGGTCAGTGGATAATCTCTCTGATTCATCTCTAAATCGGATCGGTATTCTTACGGTACGTTCTCAGTTTCGCTCTAAATCAGCTAAATCATCAGCTTCAAAGACTAGCATGAATCAGTGACCTTGGATGGTCCGAGATTCTTTGCTGAATTTCCGAGCTCAGGAGCATTTCTCTTGCTCTTACCAAACAGATTTTTCAATTTTTTTTTTAAGATTTCAATATCTGAATCATGCTGCTTCTTGCTTTGTTTCAGTATGCTGATTTTGCATTTATTGTGAATCTGATTTTGCAGTCAGGTACATAAAATGGTGATCGTGATAGCTGCATTGCTTCTCAGTGTTTTGTTGGGAGCTCTATTTTTAATCCCACGCGACCGTAAGTTTGAAGAAGTATATTTGTTTCATTTCTTCAATTCTCCAATGCATAAATTCACATCGATTTGAACAGACAATGCTATTTTCTAATGCAGGAAATGCACAAAAGGTCCAACTAAATCCTATCAACAAGGTACTTTGGATATTGAGGTTTTCTTTTCACTACATTTACTGGAAATTTGAAGACTTAGTTGTCTTTTAGATTCAATTTTATAAATGAATTGATCAATTCTTTGCCAGCTGAGAAGCTGGGACGTGAATGTTTTTAGCTTCAGTAGCATAGGTAAATATTTTGGCTTGTGCAATCTTCTATTTCCAGAATCCAGCTAAAGATAATGTATTGCTTTAATATTGAATCCTGTTAAAAATATGTTGGAGTTTTCAAATGTATAATGTTCAATCTATTCAGCCTTTTCTCAAACATGAACAAGTGGGAAAAAAAGTTGTAGAGTAAATTGATTGATTTGCTAGTCCAAGTGTTTTAAATGATTGTGCTGTTGTACTAACCAACTGCTTATAACTGGAGTGCTAATGCTTTCTGGCTTGAGTCTTTGACTAGGATTGTAAATACTGAACTTGTACAATGCACAGTTGAGAAACAGAAAAGAATCTGTAGTTTACAAGAGATTTAGTGAAACAGTTTGTGCCTTCATTCACCATAGAAACAAAGTTGTACGTCCAAATTTAATTCTCAAATGTTTGAAGCGTCTTTTAAGTTTTAACTCTCTGTGGGATTCCTTTCCGTTGTTGGTTGGATTGCTGAACTTTAGTATTGTGATTTCAAATCTCCTGGTTTAGCATAAGCTTATTTGTTGGTAATGTGCAGTTCCGGGATAGGCCTTAGTTTTTTAAATTTAAGGATCTGCGTTGATCTTATTTTTCATTAAATTTTAAATGTTGTTTCTTGCTTTTAGTCATCGAAAATCTACTACAGTAAAGATGAAGTTTCGGTTCATAACAAGAGAACTGATTGTTGGGTCATTATCAAGAACAGGGTATAATCTTCTATCTTCTGGTTTGATTTCCAAGCTCTTAGCAAAAGTTAGAGTTTGCTTGTATATTATTGTTGTACTTATAATCTTTTTTCGATTATACTTGAGCATAGGTGTATGATGTTACCTCCTATGTTGAAGAACATCCTGGAGGGGATGCCATCCTAACACATGCTGGGGATGATTCTACAGAAGGGTTTTATGGGTAAACCAAGCGGACATCACCTCATCTGTATTATGTCATGTTAAATTGATATGTCCAAACAATCTTGATTGAATTGCTCTAAAATTGTTTTGGCATAAATAGACAATAATTGAGATCACCATGCTTGATTCACTAAACAAAGACCATCGAGTGAGAGAATATTGCCTATTAACTTTCACACGCAACTAGACAGCATTCACTAAGAAAGATACCGAACCTTTTCCCTTCATTTTTACTTCAAACTATAAGGAACATGTTTGGTTTTACTCAACATACAAATAAGCTATAATATTAGATTCCATCAACGCAACAATCTTCTAAACATGTTAATTTCAACCTCGATACTTATTATGCAGGCCACAACACGCAACTCGAGTCTTTGACATGATTGAAGACTTCTATATTGGAGATCTGAAGCTGTAACATCCAAATAATGTTTGGCTTTGTGCTGTTAATTCGAGGACGTATTTCACATCGCAGATCTGGATTTGCCATTGCAGGGTATGTATGTAAAGATCTGAATTATCTAGTTAGCATTCTTGGAAAATTGTAACATCTATAGATGGTGTACACTTAATTTAAATCTTCTCCTTTTTAGATTTATATCTTTACATTTCTTGTGGATATTCTGATTTCTAATTTGCTAGAGGGGAGAGACTTTTCATTGTAGTCTTGTTTAATTTGTATTTTCTGTTGTAGCATTTGGTAAAGTGCTTTTTTATTCTTCAAGAATTGATCTGGTTTGTGTAATCTGATTTGGTGCTGTTGAGATTCTTG

mRNA sequence

GCGGAATTTTGAGAGTGATTCTGGTAAGCATTTCCCCGGCTAATTCATTTTGGCATTCAGTTCACCGGTCAGTGGATAATCTCTCTGATTCATCTCTAAATCGGATCGGTATTCTTACGTCAGGTACATAAAATGGTGATCGTGATAGCTGCATTGCTTCTCAGTGTTTTGTTGGGAGCTCTATTTTTAATCCCACGCGACCGAAATGCACAAAAGGTCCAACTAAATCCTATCAACAAGTCATCGAAAATCTACTACAGTAAAGATGAAGTTTCGGTTCATAACAAGAGAACTGATTGTTGGGTCATTATCAAGAACAGGGTGTATGATGTTACCTCCTATGTTGAAGAACATCCTGGAGGGGATGCCATCCTAACACATGCTGGGGATGATTCTACAGAAGGGTTTTATGGGCCACAACACGCAACTCGAGTCTTTGACATGATTGAAGACTTCTATATTGGAGATCTGAAGCTGTAACATCCAAATAATGTTTGGCTTTGTGCTGTTAATTCGAGGACGTATTTCACATCGCAGATCTGGATTTGCCATTGCAGGGTATGTATGTAAAGATCTGAATTATCTAGTTAGCATTCTTGGAAAATTGTAACATCTATAGATGGTGTACACTTAATTTAAATCTTCTCCTTTTTAGATTTATATCTTTACATTTCTTGTGGATATTCTGATTTCTAATTTGCTAGAGGGGAGAGACTTTTCATTGTAGTCTTGTTTAATTTGTATTTTCTGTTGTAGCATTTGGTAAAGTGCTTTTTTATTCTTCAAGAATTGATCTGGTTTGTGTAATCTGATTTGGTGCTGTTGAGATTCTTG

Coding sequence (CDS)

ATGGTGATCGTGATAGCTGCATTGCTTCTCAGTGTTTTGTTGGGAGCTCTATTTTTAATCCCACGCGACCGAAATGCACAAAAGGTCCAACTAAATCCTATCAACAAGTCATCGAAAATCTACTACAGTAAAGATGAAGTTTCGGTTCATAACAAGAGAACTGATTGTTGGGTCATTATCAAGAACAGGGTGTATGATGTTACCTCCTATGTTGAAGAACATCCTGGAGGGGATGCCATCCTAACACATGCTGGGGATGATTCTACAGAAGGGTTTTATGGGCCACAACACGCAACTCGAGTCTTTGACATGATTGAAGACTTCTATATTGGAGATCTGAAGCTGTAA

Protein sequence

MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKIYYSKDEVSVHNKRTDCWVIIKNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL*

Homology

[Close]

BLAST of CSPI02G00840 vs. ExPASy Swiss-Prot
Match: O22704 (Cytochrome B5-like protein OS=Arabidopsis thaliana OX=3702 GN=CB5LP PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 3.5e-34
Identity = 74/120 (61.67%), Postives = 86/120 (71.67%), Query Frame = 0


Query: 2   VIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKIY--------YSKDEVSVHNKR 61
           +I +  LLL  L+ ALFLI   R            SS+          YSK EV+VHNKR
Sbjct: 1   MIAVIGLLLGFLVSALFLIQGKRRRTNDNQEKKRSSSEPVEDVVRPKSYSKSEVAVHNKR 60

Query: 62  TDCWVIIKNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDL 114
            DCW+IIK++VYD+TSYVEEHPGGDAIL HAGDDST+GF+GPQHATRVFDMIEDFYIG+L
Sbjct: 61  NDCWIIIKDKVYDITSYVEEHPGGDAILDHAGDDSTDGFFGPQHATRVFDMIEDFYIGEL 120

[Close]

BLAST of CSPI02G00840 vs. ExPASy Swiss-Prot
Match: Q9ZNW2 (Acyl-lipid (9-3)-desaturase OS=Physcomitrium patens OX=3218 GN=DES6 PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 5.6e-16
Identity = 34/69 (49.28%), Postives = 52/69 (75.36%), Query Frame = 0


Query: 46  EVSVHNKRTDCWVIIKNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMI 105
           EV+VHNK +DCW+++KN+VYDV+++ +EHPGG  I T+ G D T+ F    HA   + ++
Sbjct: 109 EVAVHNKPSDCWIVVKNKVYDVSNFADEHPGGSVISTYFGRDGTDVF-SSFHAASTWKIL 168

Query: 106 EDFYIGDLK 115
           +DFYIGD++
Sbjct: 169 QDFYIGDVE 176

[Close]

BLAST of CSPI02G00840 vs. ExPASy Swiss-Prot
Match: Q9FDW8 (Cytochrome b5 isoform A OS=Arabidopsis thaliana OX=3702 GN=CB5-A PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 6.2e-15
Identity = 34/73 (46.58%), Postives = 52/73 (71.23%), Query Frame = 0


Query: 42  YSKDEVSVHNKRTDCWVIIKNRVYDVTSYVEEHPGG-DAILTHAGDDSTEGFYGPQHATR 101
           YS +E + HNK+ DCWV+I  +VYDV+SY++EHPGG D +L  AG D+T+ F    H+  
Sbjct: 8   YSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHSKD 67

Query: 102 VFDMIEDFYIGDL 114
             +++E ++IG+L
Sbjct: 68  ARELMEKYFIGEL 80

[Close]

BLAST of CSPI02G00840 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 6.9e-14
Identity = 35/78 (44.87%), Postives = 50/78 (64.10%), Query Frame = 0


Query: 37  SSKIYYSKDEVSVHNKRTDCWVIIKNRVYDVTSYVEEHPGGD-AILTHAGDDSTEGFYGP 96
           S K     +EVS HNK  DCW+II  +VYDVT ++++HPGGD  +L+  G D+T  F   
Sbjct: 3   SEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDV 62

Query: 97  QHATRVFDMIEDFYIGDL 114
            H+    DM+E +YIG++
Sbjct: 63  GHSDTARDMMEKYYIGEI 80

[Close]

BLAST of CSPI02G00840 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 77.8 bits (190), Expect = 9.0e-14
Identity = 31/81 (38.27%), Postives = 56/81 (69.14%), Query Frame = 0


Query: 34  INKSSKIYYSKDEVSVHNKRTDCWVIIKNRVYDVTSYVEEHPGG-DAILTHAGDDSTEGF 93
           ++  +K  Y+ +EV+ HN + DCW+II  +VY+V+ ++E+HPGG D +L+  G D+T+ F
Sbjct: 1   MSNDNKKVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDF 60

Query: 94  YGPQHATRVFDMIEDFYIGDL 114
               H+T    M++++Y+GD+
Sbjct: 61  EDVGHSTTARAMMDEYYVGDI 81

[Close]

BLAST of CSPI02G00840 vs. ExPASy TrEMBL
Match: A0A0A0LFU2 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000900 PE=3 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 3.1e-57
Identity = 114/115 (99.13%), Postives = 115/115 (100.00%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKIYYSKDEVSVHNKRTDCWVII 60
           MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSK+YYSKDEVSVHNKRTDCWVII
Sbjct: 121 MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSKDEVSVHNKRTDCWVII 180

Query: 61  KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 116
           KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL
Sbjct: 181 KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 235

[Close]

BLAST of CSPI02G00840 vs. ExPASy TrEMBL
Match: A0A1S3C893 (cytochrome B5-like protein OS=Cucumis melo OX=3656 GN=LOC103498106 PE=3 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 6.8e-57
Identity = 113/115 (98.26%), Postives = 115/115 (100.00%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKIYYSKDEVSVHNKRTDCWVII 60
           MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSK+YYSKDEVSVHNKRTDCWVII
Sbjct: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSKDEVSVHNKRTDCWVII 60

Query: 61  KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 116
           KN+VYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL
Sbjct: 61  KNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 115

[Close]

BLAST of CSPI02G00840 vs. ExPASy TrEMBL
Match: A0A6J1FBC0 (cytochrome B5-like protein isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443883 PE=3 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.2e-48
Identity = 97/115 (84.35%), Postives = 108/115 (93.91%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKIYYSKDEVSVHNKRTDCWVII 60
           M+I++AAL+L VL GAL LIPRDRNAQKVQLN  NK+SK+ YSKDEVSVHNKRTDCW+II
Sbjct: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNTNKASKV-YSKDEVSVHNKRTDCWIII 60

Query: 61  KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 116
           KN+VYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFY+GDL+L
Sbjct: 61  KNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 114

[Close]

BLAST of CSPI02G00840 vs. ExPASy TrEMBL
Match: A0A6J1HN83 (cytochrome B5-like protein isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465731 PE=3 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 2.6e-48
Identity = 96/115 (83.48%), Postives = 107/115 (93.04%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKIYYSKDEVSVHNKRTDCWVII 60
           M+I++AAL+L VL GAL LIPRDRNAQKVQLN  NK+SK+ Y KDEVSVHNKRTDCW+II
Sbjct: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNSTNKASKV-YGKDEVSVHNKRTDCWIII 60

Query: 61  KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 116
           KN+VYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFY+GDL+L
Sbjct: 61  KNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 114

[Close]

BLAST of CSPI02G00840 vs. ExPASy TrEMBL
Match: A0A6J1FAG5 (cytochrome B5-like protein isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443883 PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 4.9e-47
Identity = 97/118 (82.20%), Postives = 108/118 (91.53%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDR---NAQKVQLNPINKSSKIYYSKDEVSVHNKRTDCW 60
           M+I++AAL+L VL GAL LIPRDR   NAQKVQLN  NK+SK+ YSKDEVSVHNKRTDCW
Sbjct: 1   MMIIVAALVLGVLFGALILIPRDRKFGNAQKVQLNNTNKASKV-YSKDEVSVHNKRTDCW 60

Query: 61  VIIKNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 116
           +IIKN+VYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFY+GDL+L
Sbjct: 61  IIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 117

[Close]

BLAST of CSPI02G00840 vs. NCBI nr
Match: XP_004141019.1 (cytochrome B5-like protein [Cucumis sativus] >KAE8651507.1 hypothetical protein Csa_019513 [Cucumis sativus])

HSP 1 Score: 230.7 bits (587), Expect = 6.3e-57
Identity = 114/115 (99.13%), Postives = 115/115 (100.00%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKIYYSKDEVSVHNKRTDCWVII 60
           MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSK+YYSKDEVSVHNKRTDCWVII
Sbjct: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSKDEVSVHNKRTDCWVII 60

Query: 61  KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 116
           KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL
Sbjct: 61  KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 115

[Close]

BLAST of CSPI02G00840 vs. NCBI nr
Match: XP_008458811.1 (PREDICTED: cytochrome B5-like protein [Cucumis melo])

HSP 1 Score: 229.6 bits (584), Expect = 1.4e-56
Identity = 113/115 (98.26%), Postives = 115/115 (100.00%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKIYYSKDEVSVHNKRTDCWVII 60
           MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSK+YYSKDEVSVHNKRTDCWVII
Sbjct: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSKDEVSVHNKRTDCWVII 60

Query: 61  KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 116
           KN+VYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL
Sbjct: 61  KNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 115

[Close]

BLAST of CSPI02G00840 vs. NCBI nr
Match: XP_038890467.1 (cytochrome B5-like protein [Benincasa hispida] >XP_038890468.1 cytochrome B5-like protein [Benincasa hispida] >XP_038890469.1 cytochrome B5-like protein [Benincasa hispida])

HSP 1 Score: 205.3 bits (521), Expect = 2.9e-49
Identity = 102/115 (88.70%), Postives = 110/115 (95.65%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKIYYSKDEVSVHNKRTDCWVII 60
           MVIV+AAL+LSVLLGAL LIPR+RN QKVQLN INK SK+ YSKDEVSVHNKRTDCWVII
Sbjct: 1   MVIVVAALVLSVLLGALILIPRNRNVQKVQLNAINKPSKV-YSKDEVSVHNKRTDCWVII 60

Query: 61  KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 116
           KN+VY+VTSYVEEHPGGDAILTHAGDDSTEGFYGPQHA+RVFDMIEDFYIGDL+L
Sbjct: 61  KNKVYNVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHASRVFDMIEDFYIGDLRL 114

[Close]

BLAST of CSPI02G00840 vs. NCBI nr
Match: KAG7021304.1 (Cytochrome B5-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 204.1 bits (518), Expect = 6.4e-49
Identity = 98/115 (85.22%), Postives = 109/115 (94.78%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKIYYSKDEVSVHNKRTDCWVII 60
           M+I++AAL+L VL GAL LIPRDRNAQKVQLN INK+SK+ YSKDEVSVHNKRTDCW+II
Sbjct: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNINKASKV-YSKDEVSVHNKRTDCWIII 60

Query: 61  KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 116
           KN+VYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFY+GDL+L
Sbjct: 61  KNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 114

[Close]

BLAST of CSPI02G00840 vs. NCBI nr
Match: KAG6586450.1 (Mitochondrial import inner membrane translocase subunit TIM17-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 204.1 bits (518), Expect = 6.4e-49
Identity = 98/115 (85.22%), Postives = 109/115 (94.78%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKIYYSKDEVSVHNKRTDCWVII 60
           M+I++AAL+L VL GAL LIPRDRNAQKVQLN INK+SK+ YSKDEVSVHNKRTDCW+II
Sbjct: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNINKASKV-YSKDEVSVHNKRTDCWIII 60

Query: 61  KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 116
           KN+VYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFY+GDL+L
Sbjct: 61  KNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 114

[Close]

BLAST of CSPI02G00840 vs. TAIR 10
Match: AT1G60660.1 (cytochrome B5-like protein )

HSP 1 Score: 145.6 bits (366), Expect = 2.5e-35
Identity = 74/120 (61.67%), Postives = 86/120 (71.67%), Query Frame = 0


Query: 2   VIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKIY--------YSKDEVSVHNKR 61
           +I +  LLL  L+ ALFLI   R            SS+          YSK EV+VHNKR
Sbjct: 1   MIAVIGLLLGFLVSALFLIQGKRRRTNDNQEKKRSSSEPVEDVVRPKSYSKSEVAVHNKR 60

Query: 62  TDCWVIIKNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDL 114
            DCW+IIK++VYD+TSYVEEHPGGDAIL HAGDDST+GF+GPQHATRVFDMIEDFYIG+L
Sbjct: 61  NDCWIIIKDKVYDITSYVEEHPGGDAILDHAGDDSTDGFFGPQHATRVFDMIEDFYIGEL 120

[Close]

BLAST of CSPI02G00840 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 81.6 bits (200), Expect = 4.4e-16
Identity = 34/73 (46.58%), Postives = 52/73 (71.23%), Query Frame = 0


Query: 42  YSKDEVSVHNKRTDCWVIIKNRVYDVTSYVEEHPGG-DAILTHAGDDSTEGFYGPQHATR 101
           YS +E + HNK+ DCWV+I  +VYDV+SY++EHPGG D +L  AG D+T+ F    H+  
Sbjct: 8   YSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHSKD 67

Query: 102 VFDMIEDFYIGDL 114
             +++E ++IG+L
Sbjct: 68  ARELMEKYFIGEL 80

[Close]

BLAST of CSPI02G00840 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 76.6 bits (187), Expect = 1.4e-14
Identity = 30/73 (41.10%), Postives = 50/73 (68.49%), Query Frame = 0


Query: 42  YSKDEVSVHNKRTDCWVIIKNRVYDVTSYVEEHPGGD-AILTHAGDDSTEGFYGPQHATR 101
           ++  EVS H+   DCW++I  +VYDVT ++++HPGGD  ILT  G D+T+ F    H++ 
Sbjct: 8   FTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHSST 67

Query: 102 VFDMIEDFYIGDL 114
              M++++Y+GD+
Sbjct: 68  AKAMLDEYYVGDI 80

[Close]

BLAST of CSPI02G00840 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 75.9 bits (185), Expect = 2.4e-14
Identity = 33/78 (42.31%), Postives = 51/78 (65.38%), Query Frame = 0


Query: 37  SSKIYYSKDEVSVHNKRTDCWVIIKNRVYDVTSYVEEHPGGD-AILTHAGDDSTEGFYGP 96
           S +   S +EVS HNK  DCW+II  +VYDVT ++++HPGGD  +L+  G D+T  F   
Sbjct: 3   SDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDV 62

Query: 97  QHATRVFDMIEDFYIGDL 114
            H+    DM++ ++IG++
Sbjct: 63  GHSDTARDMMDKYFIGEI 80

[Close]

BLAST of CSPI02G00840 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 75.1 bits (183), Expect = 4.1e-14
Identity = 29/73 (39.73%), Postives = 50/73 (68.49%), Query Frame = 0


Query: 42  YSKDEVSVHNKRTDCWVIIKNRVYDVTSYVEEHPGG-DAILTHAGDDSTEGFYGPQHATR 101
           ++  EVS HN+  DCW++I  +VY+VT ++E+HPGG D +L+  G D+T+ F    H+  
Sbjct: 8   FTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSES 67

Query: 102 VFDMIEDFYIGDL 114
             +M+E +Y+G++
Sbjct: 68  AREMMEQYYVGEI 80

The following BLAST results are available for this feature:

BLAST of CSPI02G00840 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22704	3.5e-34	61.67	Cytochrome B5-like protein OS=Arabidopsis thaliana OX=3702 GN=CB5LP PE=2 SV=1	[more]
Q9ZNW2	5.6e-16	49.28	Acyl-lipid (9-3)-desaturase OS=Physcomitrium patens OX=3218 GN=DES6 PE=1 SV=1	[more]
Q9FDW8	6.2e-15	46.58	Cytochrome b5 isoform A OS=Arabidopsis thaliana OX=3702 GN=CB5-A PE=1 SV=1	[more]
P40934	6.9e-14	44.87	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
P49100	9.0e-14	38.27	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]

BLAST of CSPI02G00840 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LFU2	3.1e-57	99.13	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A1S3C893	6.8e-57	98.26	cytochrome B5-like protein OS=Cucumis melo OX=3656 GN=LOC103498106 PE=3 SV=1	[more]
A0A6J1FBC0	1.2e-48	84.35	cytochrome B5-like protein isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443...	[more]
A0A6J1HN83	2.6e-48	83.48	cytochrome B5-like protein isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11146573...	[more]
A0A6J1FAG5	4.9e-47	82.20	cytochrome B5-like protein isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443...	[more]

BLAST of CSPI02G00840 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141019.1	6.3e-57	99.13	cytochrome B5-like protein [Cucumis sativus] >KAE8651507.1 hypothetical protein ...	[more]
XP_008458811.1	1.4e-56	98.26	PREDICTED: cytochrome B5-like protein [Cucumis melo]	[more]
XP_038890467.1	2.9e-49	88.70	cytochrome B5-like protein [Benincasa hispida] >XP_038890468.1 cytochrome B5-lik...	[more]
KAG7021304.1	6.4e-49	85.22	Cytochrome B5-like protein [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6586450.1	6.4e-49	85.22	Mitochondrial import inner membrane translocase subunit TIM17-2, partial [Cucurb...	[more]

BLAST of CSPI02G00840 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G60660.1	2.5e-35	61.67	cytochrome B5-like protein	[more]
AT1G26340.1	4.4e-16	46.58	cytochrome B5 isoform A	[more]
AT5G48810.1	1.4e-14	41.10	cytochrome B5 isoform D	[more]
AT5G53560.1	2.4e-14	42.31	cytochrome B5 isoform E	[more]
AT2G32720.1	4.1e-14	39.73	cytochrome B5 isoform B	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 101..113 score: 33.8 coord: 74..88 score: 32.12 coord: 64..74 score: 49.45
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 42..114 e-value: 3.6E-24 score: 96.3
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 44..113 e-value: 4.3E-21 score: 74.8
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 39..114 score: 24.943094
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 34..115 e-value: 3.6E-28 score: 99.4
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 38..114
None	No IPR available	PANTHER	PTHR19359:SF95	CYTOCHROME B5-LIKE PROTEIN	coord: 2..114
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 2..114
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 70..77

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G00840.1	CSPI02G00840.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding