CSPI02G00820 (gene) Cucumber (PI 183967) v1

BLAST of CSPI02G00820 vs. ExPASy Swiss-Prot
Match: Q9SZE1 (Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g29120 PE=1 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 8.1e-122
Identity = 218/312 (69.87%), Postives = 262/312 (83.97%), Query Frame = 0

Query: 25  FLSFRLCTMSTSGDIVSPSTTRIGWIGTGIMGCLMCPHIIKAGYKLTVFNRTISKAQPLL 84
           FL   + + + S DI++PS T+IGWIGTG+MG  MC H+IKAGY +TVFNRTISKAQ L+
Sbjct: 18  FLRRSMASSTISSDIITPSNTKIGWIGTGVMGRSMCGHLIKAGYTVTVFNRTISKAQTLI 77

Query: 85  DLGANLALSPLDVAAQSNVVFSVVGYPSDVRSALLDPTSGALAGLRPGGVLIDMTTSEAS 144
           D+GAN+A SP  VA QS+VVF++VGYPSDVR  LLDP SGAL+GLR GGVL+DMTTSE S
Sbjct: 78  DMGANVADSPNSVAEQSDVVFTIVGYPSDVRHVLLDPKSGALSGLRQGGVLVDMTTSEPS 137

Query: 145 LASEVAAAATAKGCWAIDAPVSGGNRGAKNATLAIFAGGDEGVVRRLSPLFSLLGKVNYM 204
           LA E+A AA+ K C++IDAPVSGG+ GAKN  L+IFAGGDE  V+RL PLFSL+GKVN+M
Sbjct: 138 LAEEIAKAASFKNCFSIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLFSLMGKVNFM 197

Query: 205 GESGKGQFTKLANQIIIASSLVGLVEGMIYAKKAGLDVGLFLNAISTGGAGSKSLDLYGS 264
           G SGKGQF KLANQI IAS+++GLVEG+IYA KAGLDV  FL AISTG AGSKS+DLYG 
Sbjct: 198 GTSGKGQFAKLANQITIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAGSKSIDLYGD 257

Query: 265 KILERDFQPGGFVEHFVKDIGICLKDCQNMGIALPGLTLAHQLYVSLKAYGDGNLGVHAL 324
           +IL+RDF PG +V HFVKD+GICL +CQ MG+ALPGL LA QLY+SLKA+G+G+LG  AL
Sbjct: 258 RILKRDFDPGFYVNHFVKDLGICLNECQRMGLALPGLALAQQLYLSLKAHGEGDLGTQAL 317

Query: 325 ILSLERLNNVSL 337
           +L+LERLNNVS+
Sbjct: 318 LLALERLNNVSV 329

BLAST of CSPI02G00820 vs. ExPASy Swiss-Prot
Match: Q949M8 (Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71180 PE=2 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 2.2e-74
Identity = 145/297 (48.82%), Postives = 198/297 (66.67%), Query Frame = 0

Query: 39  IVSPSTTRIGWIGTGIMGCLMCPHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVA 98
           ++ PS TRIGWIG GIMG  M  HII AGY +TV+ R + K + L   GA +A SP ++A
Sbjct: 29  LIDPSKTRIGWIGIGIMGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIANSPKELA 88

Query: 99  AQSNVVFSVVGYPSDVRSALLDPTSGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGC 158
             S+VVF++VG  +DVRS LL    G L+GL PGGV +DMT+S+  LA E+ A A  + C
Sbjct: 89  EMSDVVFTIVGNFNDVRSLLLG-DDGVLSGLTPGGVTVDMTSSKPGLAREIHAEARRRNC 148

Query: 159 WAIDAPVSGGNRGAKNATLAIFAGGDEGVVRRLSPLFSLLGKVNYMGESGKGQFTKLANQ 218
           WA+DAPVSGG+ GA+  TL IFAGGD  +V  LSP+   +G V YMGE+G GQ  K+ NQ
Sbjct: 149 WAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQSCKIGNQ 208

Query: 219 IIIASSLVGLVEGMIYAKKAGLDVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVE 278
           I  AS+LVGL EG+++A+KAGLD   +L A+  G AGS  + L+G  I++RD++  GF E
Sbjct: 209 IAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYRATGFAE 268

Query: 279 HFVKDIGICLKDCQNMGIALPGLTLAHQLYVSLKAYGDGNLGVHALILSLERLNNVS 336
           + VKD+G+  +       A+PG  L+ QL+  + A GDG LG+  ++  + RLN +S
Sbjct: 269 YMVKDLGMAAE------AAMPGAALSKQLFTGMVANGDGKLGIQGVVSVIRRLNGIS 318

BLAST of CSPI02G00820 vs. ExPASy Swiss-Prot
Match: Q9C991 (Probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71170 PE=2 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 7.9e-69
Identity = 134/297 (45.12%), Postives = 191/297 (64.31%), Query Frame = 0

Query: 39  IVSPSTTRIGWIGTGIMGCLMCPHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVA 98
           ++ PS TRIGWIG GIMG  M  HI+ AGY +TV+ R + K + L   G   A SP ++ 
Sbjct: 8   LIEPSKTRIGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTANSPKELG 67

Query: 99  AQSNVVFSVVGYPSDVRSALLDPTSGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGC 158
             S+VVF++VG  +DVRS LL    G L+GL+PGGV +DMT+S+  LA E+ A A  + C
Sbjct: 68  EMSDVVFTIVGNSNDVRSLLLG-DDGVLSGLKPGGVTVDMTSSKPGLAREIYAEARRRDC 127

Query: 159 WAIDAPVSGGNRGAKNATLAIFAGGDEGVVRRLSPLFSLLGKVNYMGESGKGQFTKLANQ 218
           WA+DAPVSGG+ GA+   L IFAGGD  +V  L+P+   +G V +MG +G GQ  K+ NQ
Sbjct: 128 WAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQSCKIGNQ 187

Query: 219 IIIASSLVGLVEGMIYAKKAGLDVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVE 278
           I + S+++GL EG+++A+KAGLD   +L A+  G AGS  + L+G  +  RD++  GF E
Sbjct: 188 ICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYKATGFAE 247

Query: 279 HFVKDIGICLKDCQNMGIALPGLTLAHQLYVSLKAYGDGNLGVHALILSLERLNNVS 336
           + VKD+G+  +      +A+PG  L  QL+  + A GDG LG   ++  + RLN +S
Sbjct: 248 YMVKDLGMAAE----AAMAMPGTALNKQLFTVMVANGDGKLGFQGVVDVIRRLNGLS 299

BLAST of CSPI02G00820 vs. ExPASy Swiss-Prot
Match: O34948 (Uncharacterized oxidoreductase YkwC OS=Bacillus subtilis (strain 168) OX=224308 GN=ykwC PE=3 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 8.8e-60
Identity = 121/279 (43.37%), Postives = 183/279 (65.59%), Query Frame = 0

Query: 47  IGWIGTGIMGCLMCPHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVAAQSNVVFS 106
           IG+IG G+MG  M  HI+  G+ + V+ RT  KA+ +L  GA    +  D++ +++V+ +
Sbjct: 5   IGFIGLGVMGKSMASHILNDGHPVLVYTRTKEKAESILQKGAIWKDTVKDLSKEADVIIT 64

Query: 107 VVGYPSDVRSALLDPTSGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGCWAIDAPVS 166
           +VGYPSDV       ++G +   + G  LIDMTTS+ SLA ++A AA  K  +A+DAPVS
Sbjct: 65  MVGYPSDVEEVYFG-SNGIIENAKEGAYLIDMTTSKPSLAKKIAEAAKEKALFALDAPVS 124

Query: 167 GGNRGAKNATLAIFAGGDEGVVRRLSPLFSLLGK-VNYMGESGKGQFTKLANQIIIASSL 226
           GG+ GA+N TLAI  GG++       P+FSL+G+ + Y G +G GQ TK+ NQI IA+ +
Sbjct: 125 GGDIGAQNGTLAIMVGGEKEAFEACMPIFSLMGENIQYQGPAGSGQHTKMCNQIAIAAGM 184

Query: 227 VGLVEGMIYAKKAGLDVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVEHFVKDIG 286
           +G+ E M YA+K+GL+    L +I+TG AGS SL     ++L+ +F+PG +V+HF+KD+G
Sbjct: 185 IGVAEAMAYAQKSGLEPENVLKSITTGAAGSWSLSNLAPRMLQGNFEPGFYVKHFIKDMG 244

Query: 287 ICLKDCQNMGIALPGLTLAHQLYVSLKAYGDGNLGVHAL 325
           I L++ + MG  +PGL+LA  LY  L A G+ N G  ++
Sbjct: 245 IALEEAELMGEEMPGLSLAKSLYDKLAAQGEENSGTQSI 282

BLAST of CSPI02G00820 vs. ExPASy Swiss-Prot
Match: O33730 (Uncharacterized oxidoreductase Sfri_1503 OS=Shewanella frigidimarina (strain NCIMB 400) OX=318167 GN=Sfri_1503 PE=3 SV=2)

HSP 1 Score: 161.8 bits (408), Expect = 1.5e-38
Identity = 99/287 (34.49%), Postives = 151/287 (52.61%), Query Frame = 0

Query: 46  RIGWIGTGIMGCLMCPHIIKAGYKLTVFNRTISKA-QPLLDLGANLALSPLDVAAQSNVV 105
           ++ +IG G+MG  M  H++K G+ +TV+NRT +KA Q +   G   A +P D A   ++V
Sbjct: 3   KVAFIGLGVMGFPMAGHLVKQGHDVTVYNRTGAKATQWVEQYGGKKADTPKDAAQGQDIV 62

Query: 106 FSVVGYPSDVRSALLDPTSGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGCWAIDAP 165
           F+ VG   D+R  +L    G + G+  G +L+D TT+ A +A E+AA         +DAP
Sbjct: 63  FTCVGNDDDLRQVVLG-EHGIVHGMHAGAILVDHTTASADVAREIAAYIEPLNIAFLDAP 122

Query: 166 VSGGNRGAKNATLAIFAGGDEGVVRRLSPLFSLLGK-VNYMGESGKGQFTKLANQIIIAS 225
           VSGG  GA+N  L +  GGD+     + P+ S   +    +G  G GQ TK+ NQI IA 
Sbjct: 123 VSGGQAGAENGALTVMMGGDQAHFDTVKPVISAYSRCAELLGPVGAGQLTKMVNQICIAG 182

Query: 226 SLVGLVEGMIYAKKAGLDVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVEHFVKD 285
            + GL EG+ +AK AGLD    +  IS G A S  ++     + +  +  G  ++   KD
Sbjct: 183 VVQGLAEGLHFAKSAGLDGLKVIEVISKGAAQSWQMENRYKTMWQGQYDFGFAIDWMRKD 242

Query: 286 IGICLKDCQNMGIALPGLTLAHQLYVSLKAYGDGNLGVHALILSLER 331
           +GI L + +  G  LP   L  Q Y  ++A         +L+  LE+
Sbjct: 243 LGIALDEARRNGSHLPVAALVDQFYSEVQAMKGNRWDTSSLLARLEK 288

BLAST of CSPI02G00820 vs. ExPASy TrEMBL
Match: A0A0A0LKR4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000880 PE=4 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.5e-182
Identity = 336/340 (98.82%), Postives = 337/340 (99.12%), Query Frame = 0

Query: 1   MPLQLY-LRSLYTQSYKLTPTLSLPFLSFRLCTMSTSGDIVSPSTTRIGWIGTGIMGCLM 60
           MPLQLY LRSLYTQSYK TPTLSLPFLSFRLCTMSTSGDIVSPSTTRIGWIGTGIMGCLM
Sbjct: 1   MPLQLYLLRSLYTQSYKPTPTLSLPFLSFRLCTMSTSGDIVSPSTTRIGWIGTGIMGCLM 60

Query: 61  CPHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVAAQSNVVFSVVGYPSDVRSALL 120
           CPHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVAAQS+VVFSVVGYPSDVRSALL
Sbjct: 61  CPHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVAAQSDVVFSVVGYPSDVRSALL 120

Query: 121 DPTSGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGCWAIDAPVSGGNRGAKNATLAI 180
           DP SGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGCWAIDAPVSGGNRGAKNATLAI
Sbjct: 121 DPNSGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGCWAIDAPVSGGNRGAKNATLAI 180

Query: 181 FAGGDEGVVRRLSPLFSLLGKVNYMGESGKGQFTKLANQIIIASSLVGLVEGMIYAKKAG 240
           FAGGDEGVVRRLSPLFSLLGKVNYMGESGKGQFTKLANQIIIASSLVGLVEGMIYAKKAG
Sbjct: 181 FAGGDEGVVRRLSPLFSLLGKVNYMGESGKGQFTKLANQIIIASSLVGLVEGMIYAKKAG 240

Query: 241 LDVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVEHFVKDIGICLKDCQNMGIALP 300
           LDVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVEHFVKDIGICLKDCQNMGIALP
Sbjct: 241 LDVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVEHFVKDIGICLKDCQNMGIALP 300

Query: 301 GLTLAHQLYVSLKAYGDGNLGVHALILSLERLNNVSLDDG 340
           GLTLAHQLYVSLKAYGDGNLGVHALILSLERLNNVSLDDG
Sbjct: 301 GLTLAHQLYVSLKAYGDGNLGVHALILSLERLNNVSLDDG 340

BLAST of CSPI02G00820 vs. ExPASy TrEMBL
Match: A0A1S3C8W8 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103498120 PE=4 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.6e-163
Identity = 300/341 (87.98%), Postives = 318/341 (93.26%), Query Frame = 0

Query: 1   MPLQLYLRSLYTQSYKLTPTLSLPFLSFRLCTMSTSGDIVSPSTTRIGWIGTGIMGCLMC 60
           MPLQ  LRSLYTQS KLTPTLSLPFLSF   TMSTSGDIVSPSTTR+GWIGTG+MGC MC
Sbjct: 1   MPLQFTLRSLYTQSQKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  PHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVAAQSNVVFSVVGYPSDVRSALLD 120
            HIIKAGYKLTVFNRTISKAQPLLDLGANLA SPL VAAQS+VVFS+VGYPSDVRS LLD
Sbjct: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGCWAIDAPVSGGNRGAKNATLAIF 180
           PTSGALAGLRPGGVL+DMTTSE SLASE+AAAATAKGC AIDAPVSGG+RGAKNATLAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAATAKGCGAIDAPVSGGDRGAKNATLAIF 180

Query: 181 AGGDEGVVRRLSPLFSLLGKVNYMGESGKGQFTKLANQIIIASSLVGLVEGMIYAKKAGL 240
           AGGDEG VRRLSPLFSLLGKVNYMGESGKGQF KLANQI IAS++VGLVEGMIYAKKAGL
Sbjct: 181 AGGDEGEVRRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240

Query: 241 DVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVEHFVKDIGICLKDCQNMGIALPG 300
           DVGLFLNAISTG AGSKSLDLYGS+IL+RDF+PG FV HFVKD+GICLK+CQNMG+ALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LTLAHQLYVSLKAYGDGNLGVHALILSLERLNNVSLDDGGA 342
           L LA QLYVSLKAYG+GNLG  ALILS+ERLNNVSLDDGG+
Sbjct: 301 LALAQQLYVSLKAYGEGNLGTQALILSVERLNNVSLDDGGS 341

BLAST of CSPI02G00820 vs. ExPASy TrEMBL
Match: A0A0A0LIQ8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000840 PE=4 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 5.9e-160
Identity = 294/341 (86.22%), Postives = 316/341 (92.67%), Query Frame = 0

Query: 1   MPLQLYLRSLYTQSYKLTPTLSLPFLSFRLCTMSTSGDIVSPSTTRIGWIGTGIMGCLMC 60
           MP+Q  LRSLYTQS+KLTPTLSLPFLSF   TMSTSGDIVSPSTTR+GWIGTG+MGC MC
Sbjct: 1   MPIQFSLRSLYTQSHKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  PHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVAAQSNVVFSVVGYPSDVRSALLD 120
            HIIKAGYKLTVFNRTISKAQPLLDLGANLA SPL VAAQS+VVFS+VGYPSDVRS LLD
Sbjct: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGCWAIDAPVSGGNRGAKNATLAIF 180
           P+SGALAGLRPGGVLIDMTTSE SLASE+AA+ATAKGC AIDAPVSGG+RGAKNATLAIF
Sbjct: 121 PSSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIF 180

Query: 181 AGGDEGVVRRLSPLFSLLGKVNYMGESGKGQFTKLANQIIIASSLVGLVEGMIYAKKAGL 240
           AGGDE  V+RLSPLFSLLGKVNYMGESGKGQF KLANQI IAS++VGLVEGMIYAKKAGL
Sbjct: 181 AGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240

Query: 241 DVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVEHFVKDIGICLKDCQNMGIALPG 300
           DVGLFLNAISTG AGSKSLDLYGS+IL+RDF+PG FV HFVKD+GICLK+CQNMG+ALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LTLAHQLYVSLKAYGDGNLGVHALILSLERLNNVSLDDGGA 342
           L LA QLYVSLKAYG+GNLG  ALILSLERLNNVSL+  G+
Sbjct: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLEGSGS 341

BLAST of CSPI02G00820 vs. ExPASy TrEMBL
Match: A0A6J1G2N8 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111450245 PE=4 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.9e-154
Identity = 282/336 (83.93%), Postives = 309/336 (91.96%), Query Frame = 0

Query: 1   MPLQLYLRSLYTQSYKLTPTLSLPFLSFRLCTMSTSGDIVSPSTTRIGWIGTGIMGCLMC 60
           MPLQ+ LRSLYTQS+KL PTLSLPFL +   TM+TSGDIVSP+TTR+GWIGTG+MGC MC
Sbjct: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMC 60

Query: 61  PHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVAAQSNVVFSVVGYPSDVRSALLD 120
            HIIKAG+KLTVFNRTISKAQPLLDLGANLA SPL VAAQS+VVFS+VGYPSDVRS LLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGCWAIDAPVSGGNRGAKNATLAIF 180
           PTSGALAGLRPGGVL+DMTTSE SLASE+AAA  AKGC A+DAPVSGG+RGAKNA+LAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGDEGVVRRLSPLFSLLGKVNYMGESGKGQFTKLANQIIIASSLVGLVEGMIYAKKAGL 240
           AGG+EG VRRL+PLFSLLGKVNYMGESGKGQF KLANQI IAS++VGLVEGMIYA KAGL
Sbjct: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVEHFVKDIGICLKDCQNMGIALPG 300
           DV LFLNAISTG AGSKSLDLYGS+IL+RDF+PG FV HFVKD+GICLK+CQNMG+ALPG
Sbjct: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LTLAHQLYVSLKAYGDGNLGVHALILSLERLNNVSL 337
           L LA QLYVSLKA+G+GNLG  ALILSLERLNNVSL
Sbjct: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSL 336

BLAST of CSPI02G00820 vs. ExPASy TrEMBL
Match: A0A6J1KJW1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111493847 PE=4 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 2.0e-152
Identity = 280/336 (83.33%), Postives = 308/336 (91.67%), Query Frame = 0

Query: 1   MPLQLYLRSLYTQSYKLTPTLSLPFLSFRLCTMSTSGDIVSPSTTRIGWIGTGIMGCLMC 60
           MPLQ+ LRSLYTQS+KL PTLSL FL     TM+TSGDIV+P+TTR+GWIGTG+MGC MC
Sbjct: 1   MPLQISLRSLYTQSHKLAPTLSLSFLPHLRRTMATSGDIVNPTTTRVGWIGTGVMGCSMC 60

Query: 61  PHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVAAQSNVVFSVVGYPSDVRSALLD 120
            HIIKAG+KLTVFNRTISKAQPL+DLGANLA SPL VAAQS+VVFS+VGYPSDVRS LLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLVDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGCWAIDAPVSGGNRGAKNATLAIF 180
           PTSGALAGLRPGGVL+DMTTSE SLASE+AAAA AKGC A+DAPVSGG+RGAKNA+LAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCKAVDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGDEGVVRRLSPLFSLLGKVNYMGESGKGQFTKLANQIIIASSLVGLVEGMIYAKKAGL 240
           AGG+EG VRRL+PLFSLLGKVNYMGESGKGQF KLANQI IAS++VGLVEGMIYA KAGL
Sbjct: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVEHFVKDIGICLKDCQNMGIALPG 300
           DV LFLNAISTG AGSKSLDLYGS+IL+RDF+PG FV HFVKD+GICLK+CQNMG+ALPG
Sbjct: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LTLAHQLYVSLKAYGDGNLGVHALILSLERLNNVSL 337
           L LA QLYVSLKA+G+GNLG  ALILSLERLNNVSL
Sbjct: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSL 336

BLAST of CSPI02G00820 vs. NCBI nr
Match: XP_004141021.2 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucumis sativus] >KGN60581.1 hypothetical protein Csa_019337 [Cucumis sativus])

HSP 1 Score: 648.7 bits (1672), Expect = 3.0e-182
Identity = 336/340 (98.82%), Postives = 337/340 (99.12%), Query Frame = 0

Query: 1   MPLQLY-LRSLYTQSYKLTPTLSLPFLSFRLCTMSTSGDIVSPSTTRIGWIGTGIMGCLM 60
           MPLQLY LRSLYTQSYK TPTLSLPFLSFRLCTMSTSGDIVSPSTTRIGWIGTGIMGCLM
Sbjct: 1   MPLQLYLLRSLYTQSYKPTPTLSLPFLSFRLCTMSTSGDIVSPSTTRIGWIGTGIMGCLM 60

Query: 61  CPHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVAAQSNVVFSVVGYPSDVRSALL 120
           CPHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVAAQS+VVFSVVGYPSDVRSALL
Sbjct: 61  CPHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVAAQSDVVFSVVGYPSDVRSALL 120

Query: 121 DPTSGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGCWAIDAPVSGGNRGAKNATLAI 180
           DP SGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGCWAIDAPVSGGNRGAKNATLAI
Sbjct: 121 DPNSGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGCWAIDAPVSGGNRGAKNATLAI 180

Query: 181 FAGGDEGVVRRLSPLFSLLGKVNYMGESGKGQFTKLANQIIIASSLVGLVEGMIYAKKAG 240
           FAGGDEGVVRRLSPLFSLLGKVNYMGESGKGQFTKLANQIIIASSLVGLVEGMIYAKKAG
Sbjct: 181 FAGGDEGVVRRLSPLFSLLGKVNYMGESGKGQFTKLANQIIIASSLVGLVEGMIYAKKAG 240

Query: 241 LDVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVEHFVKDIGICLKDCQNMGIALP 300
           LDVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVEHFVKDIGICLKDCQNMGIALP
Sbjct: 241 LDVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVEHFVKDIGICLKDCQNMGIALP 300

Query: 301 GLTLAHQLYVSLKAYGDGNLGVHALILSLERLNNVSLDDG 340
           GLTLAHQLYVSLKAYGDGNLGVHALILSLERLNNVSLDDG
Sbjct: 301 GLTLAHQLYVSLKAYGDGNLGVHALILSLERLNNVSLDDG 340

BLAST of CSPI02G00820 vs. NCBI nr
Match: XP_008458836.1 (PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucumis melo])

HSP 1 Score: 584.7 bits (1506), Expect = 5.3e-163
Identity = 300/341 (87.98%), Postives = 318/341 (93.26%), Query Frame = 0

Query: 1   MPLQLYLRSLYTQSYKLTPTLSLPFLSFRLCTMSTSGDIVSPSTTRIGWIGTGIMGCLMC 60
           MPLQ  LRSLYTQS KLTPTLSLPFLSF   TMSTSGDIVSPSTTR+GWIGTG+MGC MC
Sbjct: 1   MPLQFTLRSLYTQSQKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  PHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVAAQSNVVFSVVGYPSDVRSALLD 120
            HIIKAGYKLTVFNRTISKAQPLLDLGANLA SPL VAAQS+VVFS+VGYPSDVRS LLD
Sbjct: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGCWAIDAPVSGGNRGAKNATLAIF 180
           PTSGALAGLRPGGVL+DMTTSE SLASE+AAAATAKGC AIDAPVSGG+RGAKNATLAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAATAKGCGAIDAPVSGGDRGAKNATLAIF 180

Query: 181 AGGDEGVVRRLSPLFSLLGKVNYMGESGKGQFTKLANQIIIASSLVGLVEGMIYAKKAGL 240
           AGGDEG VRRLSPLFSLLGKVNYMGESGKGQF KLANQI IAS++VGLVEGMIYAKKAGL
Sbjct: 181 AGGDEGEVRRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240

Query: 241 DVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVEHFVKDIGICLKDCQNMGIALPG 300
           DVGLFLNAISTG AGSKSLDLYGS+IL+RDF+PG FV HFVKD+GICLK+CQNMG+ALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LTLAHQLYVSLKAYGDGNLGVHALILSLERLNNVSLDDGGA 342
           L LA QLYVSLKAYG+GNLG  ALILS+ERLNNVSLDDGG+
Sbjct: 301 LALAQQLYVSLKAYGEGNLGTQALILSVERLNNVSLDDGGS 341

BLAST of CSPI02G00820 vs. NCBI nr
Match: XP_004141022.2 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucumis sativus] >KGN60577.1 hypothetical protein Csa_019202 [Cucumis sativus])

HSP 1 Score: 573.5 bits (1477), Expect = 1.2e-159
Identity = 294/341 (86.22%), Postives = 316/341 (92.67%), Query Frame = 0

Query: 1   MPLQLYLRSLYTQSYKLTPTLSLPFLSFRLCTMSTSGDIVSPSTTRIGWIGTGIMGCLMC 60
           MP+Q  LRSLYTQS+KLTPTLSLPFLSF   TMSTSGDIVSPSTTR+GWIGTG+MGC MC
Sbjct: 1   MPIQFSLRSLYTQSHKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  PHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVAAQSNVVFSVVGYPSDVRSALLD 120
            HIIKAGYKLTVFNRTISKAQPLLDLGANLA SPL VAAQS+VVFS+VGYPSDVRS LLD
Sbjct: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGCWAIDAPVSGGNRGAKNATLAIF 180
           P+SGALAGLRPGGVLIDMTTSE SLASE+AA+ATAKGC AIDAPVSGG+RGAKNATLAIF
Sbjct: 121 PSSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIF 180

Query: 181 AGGDEGVVRRLSPLFSLLGKVNYMGESGKGQFTKLANQIIIASSLVGLVEGMIYAKKAGL 240
           AGGDE  V+RLSPLFSLLGKVNYMGESGKGQF KLANQI IAS++VGLVEGMIYAKKAGL
Sbjct: 181 AGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240

Query: 241 DVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVEHFVKDIGICLKDCQNMGIALPG 300
           DVGLFLNAISTG AGSKSLDLYGS+IL+RDF+PG FV HFVKD+GICLK+CQNMG+ALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LTLAHQLYVSLKAYGDGNLGVHALILSLERLNNVSLDDGGA 342
           L LA QLYVSLKAYG+GNLG  ALILSLERLNNVSL+  G+
Sbjct: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLEGSGS 341

BLAST of CSPI02G00820 vs. NCBI nr
Match: XP_038889572.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Benincasa hispida])

HSP 1 Score: 563.9 bits (1452), Expect = 9.7e-157
Identity = 291/345 (84.35%), Postives = 314/345 (91.01%), Query Frame = 0

Query: 1   MPLQLYLRSLYTQSYKLTPTLSLPFLSFRLCTMSTSGDIVSPSTTRIGWIGTGIMGCLMC 60
           MPLQ+ LRSLYTQS +LT TLSLPFLSF  CTMSTS DIVSPSTTR+GWIGTG+MGC MC
Sbjct: 1   MPLQISLRSLYTQSNQLTHTLSLPFLSFLRCTMSTSRDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  PHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVAAQSNVVFSVVGYPSDVRSALLD 120
            HIIKAG+KLTVFNRTISKAQPLLDLGANLA SPL VAAQS++VFS+VGYPSDVRS LLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDIVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGCWAIDAPVSGGNRGAKNATLAIF 180
           PTSGALAGLRPGGVL+DMTTSE SLASE+AAAATAKGC AIDAPVSGG+RGAKNA+LAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAATAKGCGAIDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGDEGVVRRLSPLFSLLGKVNYMGESGKGQFTKLANQIIIASSLVGLVEGMIYAKKAGL 240
           AGGDE  VRRL PLFSLLGKVNYMGE+GKGQF KLANQI IAS++VGLVEGMIYA KAGL
Sbjct: 181 AGGDESEVRRLKPLFSLLGKVNYMGETGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVEHFVKDIGICLKDCQNMGIALPG 300
           DVGLFLNAISTG AGSKSLDLYGS+IL+RDF+PG FV HFVKD+GICLK+CQNMG+ALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LTLAHQLYVSLKAYGDGNLGVHALILSLERLNNVSLD-----DGG 341
           L LA QLYVSLKAYG+GNLG  ALILSLERLNNVSL+     DGG
Sbjct: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLESAPGGDGG 345

BLAST of CSPI02G00820 vs. NCBI nr
Match: XP_023545872.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 555.1 bits (1429), Expect = 4.5e-154
Identity = 283/336 (84.23%), Postives = 310/336 (92.26%), Query Frame = 0

Query: 1   MPLQLYLRSLYTQSYKLTPTLSLPFLSFRLCTMSTSGDIVSPSTTRIGWIGTGIMGCLMC 60
           MPLQ+ LRSLYTQS+KL PTLSLPFL +   TM+TSGDIVSPSTTR+GWIGTG+MGC MC
Sbjct: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMTTSGDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  PHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVAAQSNVVFSVVGYPSDVRSALLD 120
            HIIKAG+KLTVFNRTISKAQPLLDLGANLA SPL VAAQS+VVFS+VGYPSDVRS LLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGCWAIDAPVSGGNRGAKNATLAIF 180
           PTSGALAGLRPGGVL+DMTTSE SLASE+AAAA AKGC A+DAPVSGG+RGAKNA+LAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCKAVDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGDEGVVRRLSPLFSLLGKVNYMGESGKGQFTKLANQIIIASSLVGLVEGMIYAKKAGL 240
           AGG+EG V+RL+PLFSLLGKVNYMGESGKGQF KLANQI IAS++VGLVEGMIYA KAGL
Sbjct: 181 AGGNEGEVQRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVEHFVKDIGICLKDCQNMGIALPG 300
           DV LFLNAISTG AGSKSLDLYGS+IL+RDF+PG FV HFVKD+GICLK+CQNMG+ALPG
Sbjct: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LTLAHQLYVSLKAYGDGNLGVHALILSLERLNNVSL 337
           L LA QLYVSLKA+G+GNLG  ALILSLERLNNVSL
Sbjct: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSL 336

BLAST of CSPI02G00820 vs. TAIR 10
Match: AT4G29120.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 438.3 bits (1126), Expect = 5.7e-123
Identity = 218/312 (69.87%), Postives = 262/312 (83.97%), Query Frame = 0

Query: 25  FLSFRLCTMSTSGDIVSPSTTRIGWIGTGIMGCLMCPHIIKAGYKLTVFNRTISKAQPLL 84
           FL   + + + S DI++PS T+IGWIGTG+MG  MC H+IKAGY +TVFNRTISKAQ L+
Sbjct: 18  FLRRSMASSTISSDIITPSNTKIGWIGTGVMGRSMCGHLIKAGYTVTVFNRTISKAQTLI 77

Query: 85  DLGANLALSPLDVAAQSNVVFSVVGYPSDVRSALLDPTSGALAGLRPGGVLIDMTTSEAS 144
           D+GAN+A SP  VA QS+VVF++VGYPSDVR  LLDP SGAL+GLR GGVL+DMTTSE S
Sbjct: 78  DMGANVADSPNSVAEQSDVVFTIVGYPSDVRHVLLDPKSGALSGLRQGGVLVDMTTSEPS 137

Query: 145 LASEVAAAATAKGCWAIDAPVSGGNRGAKNATLAIFAGGDEGVVRRLSPLFSLLGKVNYM 204
           LA E+A AA+ K C++IDAPVSGG+ GAKN  L+IFAGGDE  V+RL PLFSL+GKVN+M
Sbjct: 138 LAEEIAKAASFKNCFSIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLFSLMGKVNFM 197

Query: 205 GESGKGQFTKLANQIIIASSLVGLVEGMIYAKKAGLDVGLFLNAISTGGAGSKSLDLYGS 264
           G SGKGQF KLANQI IAS+++GLVEG+IYA KAGLDV  FL AISTG AGSKS+DLYG 
Sbjct: 198 GTSGKGQFAKLANQITIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAGSKSIDLYGD 257

Query: 265 KILERDFQPGGFVEHFVKDIGICLKDCQNMGIALPGLTLAHQLYVSLKAYGDGNLGVHAL 324
           +IL+RDF PG +V HFVKD+GICL +CQ MG+ALPGL LA QLY+SLKA+G+G+LG  AL
Sbjct: 258 RILKRDFDPGFYVNHFVKDLGICLNECQRMGLALPGLALAQQLYLSLKAHGEGDLGTQAL 317

Query: 325 ILSLERLNNVSL 337
           +L+LERLNNVS+
Sbjct: 318 LLALERLNNVSV 329

BLAST of CSPI02G00820 vs. TAIR 10
Match: AT1G71180.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 280.8 bits (717), Expect = 1.5e-75
Identity = 145/297 (48.82%), Postives = 198/297 (66.67%), Query Frame = 0

Query: 39  IVSPSTTRIGWIGTGIMGCLMCPHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVA 98
           ++ PS TRIGWIG GIMG  M  HII AGY +TV+ R + K + L   GA +A SP ++A
Sbjct: 29  LIDPSKTRIGWIGIGIMGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIANSPKELA 88

Query: 99  AQSNVVFSVVGYPSDVRSALLDPTSGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGC 158
             S+VVF++VG  +DVRS LL    G L+GL PGGV +DMT+S+  LA E+ A A  + C
Sbjct: 89  EMSDVVFTIVGNFNDVRSLLLG-DDGVLSGLTPGGVTVDMTSSKPGLAREIHAEARRRNC 148

Query: 159 WAIDAPVSGGNRGAKNATLAIFAGGDEGVVRRLSPLFSLLGKVNYMGESGKGQFTKLANQ 218
           WA+DAPVSGG+ GA+  TL IFAGGD  +V  LSP+   +G V YMGE+G GQ  K+ NQ
Sbjct: 149 WAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQSCKIGNQ 208

Query: 219 IIIASSLVGLVEGMIYAKKAGLDVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVE 278
           I  AS+LVGL EG+++A+KAGLD   +L A+  G AGS  + L+G  I++RD++  GF E
Sbjct: 209 IAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYRATGFAE 268

Query: 279 HFVKDIGICLKDCQNMGIALPGLTLAHQLYVSLKAYGDGNLGVHALILSLERLNNVS 336
           + VKD+G+  +       A+PG  L+ QL+  + A GDG LG+  ++  + RLN +S
Sbjct: 269 YMVKDLGMAAE------AAMPGAALSKQLFTGMVANGDGKLGIQGVVSVIRRLNGIS 318

BLAST of CSPI02G00820 vs. TAIR 10
Match: AT1G71170.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 262.3 bits (669), Expect = 5.6e-70
Identity = 134/297 (45.12%), Postives = 191/297 (64.31%), Query Frame = 0

Query: 39  IVSPSTTRIGWIGTGIMGCLMCPHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVA 98
           ++ PS TRIGWIG GIMG  M  HI+ AGY +TV+ R + K + L   G   A SP ++ 
Sbjct: 8   LIEPSKTRIGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTANSPKELG 67

Query: 99  AQSNVVFSVVGYPSDVRSALLDPTSGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGC 158
             S+VVF++VG  +DVRS LL    G L+GL+PGGV +DMT+S+  LA E+ A A  + C
Sbjct: 68  EMSDVVFTIVGNSNDVRSLLLG-DDGVLSGLKPGGVTVDMTSSKPGLAREIYAEARRRDC 127

Query: 159 WAIDAPVSGGNRGAKNATLAIFAGGDEGVVRRLSPLFSLLGKVNYMGESGKGQFTKLANQ 218
           WA+DAPVSGG+ GA+   L IFAGGD  +V  L+P+   +G V +MG +G GQ  K+ NQ
Sbjct: 128 WAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQSCKIGNQ 187

Query: 219 IIIASSLVGLVEGMIYAKKAGLDVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVE 278
           I + S+++GL EG+++A+KAGLD   +L A+  G AGS  + L+G  +  RD++  GF E
Sbjct: 188 ICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYKATGFAE 247

Query: 279 HFVKDIGICLKDCQNMGIALPGLTLAHQLYVSLKAYGDGNLGVHALILSLERLNNVS 336
           + VKD+G+  +      +A+PG  L  QL+  + A GDG LG   ++  + RLN +S
Sbjct: 248 YMVKDLGMAAE----AAMAMPGTALNKQLFTVMVANGDGKLGFQGVVDVIRRLNGLS 299

BLAST of CSPI02G00820 vs. TAIR 10
Match: AT1G17650.1 (glyoxylate reductase 2 )

HSP 1 Score: 138.3 bits (347), Expect = 1.2e-32
Identity = 91/322 (28.26%), Postives = 163/322 (50.62%), Query Frame = 0

Query: 17  LTPTLSLP--------FLSFRLCTMSTSGDIVSPSTTRIGWIGTGIMGCLMCPHIIKAGY 76
           ++P LS P        + S +  T ST  ++    T  IG++G GIMG  M  ++IKAG 
Sbjct: 34  ISPFLSKPQICLAYRVYSSLQSTTPSTRDEL---GTVSIGFLGMGIMGSPMAQNLIKAGC 93

Query: 77  KLTVFNRTISKAQPLLDLGANLALSPLDVAAQSNVVFSVVGYPSDVRSALLDPTSGALAG 136
            +TV+NRT SK  PL+ LGA    SP +V A  ++ F+++  P           +GA+ G
Sbjct: 94  DVTVWNRTKSKCDPLVGLGAKYKSSPEEVTATCDLTFAMLADPESAIDVACG-KNGAIFG 153

Query: 137 LRPGGVLIDMTTSEASLASEVAAAATAKGCWAIDAPVSGGNRGAKNATLAIFAGGDEGVV 196
           +  G   +D++T + + +  ++      G   ++APVSG  + A++  L     GD+ + 
Sbjct: 154 ISSGKGYVDVSTVDVASSILISKQIKDTGALFLEAPVSGSKKPAEDGQLIFLTAGDKPLY 213

Query: 197 RRLSPLFSLLGKVN-YMGESGKGQFTKLANQIIIASSLVGLVEGMIYAKKAGLDVGLFLN 256
            + +P   ++GK   Y+GE G G   KL   +I+ S +    EG++ ++K GLD  + + 
Sbjct: 214 EKAAPFLDIMGKSKFYLGEVGNGAAMKLVVNMIMGSMMASFAEGILLSQKVGLDPNVLVE 273

Query: 257 AISTGGAGSKSLDLYGSKILERDFQPGGFVEHFVKDIGICLKDCQNMGIALPGLTLAHQL 316
            +S G   +    L G  +++  +     ++H  KD+ + L   +++  + P    A++L
Sbjct: 274 VVSQGAINAPMYSLKGPSMIKSVYPTAFPLKHQQKDMRLALGLAESVSQSTPIAAAANEL 333

Query: 317 YVSLKAYGDGNLGVHALILSLE 330
           Y   K+YG  +    A+I +L+
Sbjct: 334 YKVAKSYGLSDEDFSAVIEALK 351

BLAST of CSPI02G00820 vs. TAIR 10
Match: AT3G25530.1 (glyoxylate reductase 1 )

HSP 1 Score: 132.5 bits (332), Expect = 6.7e-31
Identity = 82/280 (29.29%), Postives = 145/280 (51.79%), Query Frame = 0

Query: 47  IGWIGTGIMGCLMCPHIIKAGYKLTVFNRTISKAQPLLDLGANLALSPLDVAAQSNVVFS 106
           +G++G GIMG  M  +++K G+K+TV+NRT+SK   L++ GA++  SP +V  +     +
Sbjct: 3   VGFLGLGIMGKAMSMNLLKNGFKVTVWNRTLSKCDELVEHGASVCESPAEVIKKCKYTIA 62

Query: 107 VVGYPSDVRSALLDPTSGALAGLRPGGVLIDMTTSEASLASEVAAAATAKGCWAIDAPVS 166
           ++  P    S + D   G L  +  G   IDM+T +A  + ++  A T KG   ++ PVS
Sbjct: 63  MLSDPCAALSVVFD-KGGVLEQICEGKGYIDMSTVDAETSLKINEAITGKGGRFVEGPVS 122

Query: 167 GGNRGAKNATLAIFAGGDEGVVRRLSPLFSLLGKVN-YMGESGKGQFTKLANQIIIASSL 226
           G  + A++  L I A GD+ +     P F +LGK + Y+G+ G G   KL   +I+ S +
Sbjct: 123 GSKKPAEDGQLIILAAGDKALFEESIPAFDVLGKRSFYLGQVGNGAKMKLIVNMIMGSMM 182

Query: 227 VGLVEGMIYAKKAGLDVGLFLNAISTGGAGSKSLDLYGSKILERDFQPGGFVEHFVKDIG 286
               EG++ A K+GL     L+ +  G   +      G  + +  + P   ++H  KD+ 
Sbjct: 183 NAFSEGLVLADKSGLSSDTLLDILDLGAMTNPMFKGKGPSMNKSSYPPAFPLKHQQKDMR 242

Query: 287 ICLKDCQNMGIALPGLTLAHQLYVSLKAYGDGNLGVHALI 326
           + L       +++P    A++ +   ++ G G+L   A+I
Sbjct: 243 LALALGDENAVSMPVAAAANEAFKKARSLGLGDLDFSAVI 281