CSPI02G00600 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G00600
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionVacuolar protein sorting-associated protein 32-like protein 2
LocationChr2: 354684 .. 358312 (-)
RNA-Seq ExpressionCSPI02G00600
SyntenyCSPI02G00600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GATCTATTTTTCGTTGAATTGATTATTATTTGCTACTTCATAGTGGGCTTGGAGGTATGCTAACTAGTTCGGCCCATATACATGTCCAGGAGCTCAACTGGGAGTTTGGGCCGATTCATAGTCGGCCTGTAATGAAAATATGTATTGAGCTCGGCCCAAATTCTCATTTAAATAGAATTGGCGGAGTGAAAATTGGGAGTTTTGATTTGATGAACGGAAACCAATAAGTAGATTGCCGCGTGGAGAAGAAACAGCGACTTGAAAAGAAAGGGAACTCCAAATCTTCGACCTCCGTTTTCTGGAGCGTCTGACAAACCCTTTCTACAATTCTACTTCAAATCTTTTCATTGAATCTTCCGATTCATTCTCCGTTTTGTGAATTCCTTTTGGATTCTCATCTTTCTGTTTCAATCTCTCTGGTTTCTCGATTTTTGGTACACATTGATTACTTTTTCCTTTTCCTTTTCTTTATTCTTGATGTTGAATCCGCAGGTTGAAACTAAGCGTTTGAATCTTATTTTGTTCGTTGTTGTAGAAGATCGAGAGAGGATTCCATTTGCTTTGCATCGACAATGTTTAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTTTGGCTACGTTAGAAAAGCTAAGCGAGGTGCGGCCTCATTTTGTGATTTTAGTTTTTTAGATTGATATTCTTTTCATGGAAACGATATTTTATTGTGTGTCGTTTGTTTTGAGCTCTTGTTCTATTTCAACAATTTTGATGTATGCTTGACTTCTCGATCATTCATCTTTTCCAAATGGCAGTGTTTAGGACATCGGTTTGATTAGTTCTCACGTCTTCTTGACCGTGTATTAGTGTTTATTTGGTCTTTACTTGGTCAAGTGGTGGTTTCCGGTTGAAAATTTTTCCGTGTTTGAATTGGATTAATTGTTAATTTTTTTTTTTTTTTTGTTTGTTCTGCGAATGGTAATCGATGTGTTTTAGGTGAAATTACACTTATGTAAGTTAATTGTTTGTTATTTTTCTATGCTACCACCATATTGAAAGAATAGATAATTGGAATCATGTCCGAAATGATTGTAATTGTGATTGCATAGACTTTTGTAGTCTTGCAACGTTTTTCGTAATTGATACCAATGTATCTGGGTACTATTTTTTATTGTCTGTAATTCTCTGTTTAGACACTTGAAATGCTGGAGAAGAAAGAGAATGTTCTTGTGAAGAAGGCAGCTGCAGAGGTCGAAAGAGCAAAAGAATTCACAAGAGGGAAGAATAAACGAGGTATGGTCTGAAATTGTGAGTACAGTAGCAGTATGGATTTTTTGCTTTGTTAGCATTTCTTATATGTGTGACGTGTGAATAAGATTTCATTAATACCTATCTTGTTCGCTCTTAGGTGTAAAATTGTTGGTAGCACCATATCTGTTTTCTCATGATTTGGTATTGCAATTTGTGCATATGTCAATCCTTTGCAATGTGATAATCTGCAGAAATTTTTTAATGCTGTCTTTCGTATACAGTTTTGTTCATTATTATTAGGAGAATCTTTCAAGGAACCCTTCGACTCTGTATCTCAATGAAATTAATTTTGATCGTATGAAAATTGTCCATGTTAATCTCAGGACTCTGTATAGAGTCAAGGTTTTACTGTTATATCAGTAAAATTGCTAGATAGGCTTGCATGGTTGGTCTTTCTGCCTTGTGCTTTTCTGGCTTTTGCTACTGTCTTTATGGCTTGCAGTTGAACTGTAACCTTTCTATTTCCTTTATAATAGGGTTGTGTATGGCTAAGATTTGTAGCCTTGATTACTTCAATGCTTCGTTTTCGCTTTCTGTCCATTTTGGATTTTTTTTTAGATTTGCCTTGTTCTTATCATTATTAGTAGCTTTGGGTGATCGTTTTCAAACAGTTTAATTTTTTTTGTTCTTTGTCTCTTTTGTTGAGAACTGTAGCTTTGGAGATTGTTCTGTTTGCCTTTTTTTTTTATCTATATTTTCCCTTTTCATTCTTCTTTTTTATATATTTTTATCAGAATCATTGTTACCATTGTTTTTGCTTATCCTTAACTCCCTATTTTACCTTTTGTCCATATCTTAGAGGCCTTGGATATTGAATAGTTATGTTTAGGAATTAAATATTTGTTCTATACAAAAATTAACGTCTGAAAATTAATCAACTATTTGTAAATCTTAGAGGTTATATATAAAAATCGAGTTTACATAGTTAATTAGATTGGACCTAATAGATTACTGATACTGAATTATATAAATTATCCATCATAGATCAAACCTTATGTAATTCAGTATATTATTATGTTCTATTTGGCTTCTGTTTTTAGGAGTGTCCATTCTTATTCATTATAATTCGTTTCCAGCGGCTATACAATGTTTGAAGAGAAAAAGGCTTTATGAACAGCAAATAGAGCAGCTTGGAAATTTCCAATTGCGCATCCATGATCAAGTGAGTTGATGTTGTTATTTTTACAGATTGATGAGACATCAATTTCTTTCTTTTTCTCCCTATAACTACAATTCATGGAAGTGTTTGTTAACTTTATTCAAAAAATTTCTTACTTTTGCAGATGATTATGTTAGAAGGGGCAAAAGCTACAACGGAGACCGTAGATGCTCTACGAACAGGAGCATCTGCAATGAAGGCAATGCAGAAAGCAACGTACGCCTGCTTTACTTTCGCTCATTTGGTTTACTGTAGATCCTAGTTTATTGTTGTTTTTTCTGTTTACTTTGCCGGATTATAAAAAGAAAAGCTTTACAACAAACCTGAACTTATTGATATTAGGCTAACAGGCTTCGATTGACAGCAGCTTTTGAAAAATGCTTGTGAAATTTTGTGTAAAGTTTTTATCGTGAAGTGTTAGACATGCACATCTTATATGGAGCTTTGTTGTTCAGGAATATTGATGATGTAGACAAGACGATGGATGAGATCAACGAGCAGACTGAGAATATGAAACAGATACAGGAAGCGTTATCAGCTCCAATTGGTGCTGCAGCTGATTTCGATGAGGTAGTTAATCTTTGAATAGTATGTTGAGGTTTGTAAGCTTTTATATATTATCTCATCTCATTAAAGGATGTGGTTGTGGTGGTACTCAGGATGAATTGGAGGCAGAGCTTGAAGATCTAGAAAGTGCGGAGTTGGAAGAGCAACTGCTGCAACCAGCATCAACAGCTCCTGCTGCACCTGTCTCAGTCCCGGGAGGCCGAGTTCCTGCACGCCCTGCTCCTCAAAAGAGAACTGCTGAAGAGGACGAACTCGCTGCTCTGCAAGCTGAGATGGCACTCTAAACAGGTTTATTTATTTATTTATTTGAGTTCCACACGAATAGAAAAAGGCAAGTTGTGTTTGTTGTAACGGAGTTTTGATTGGTAATTGGCCAGGTAAAATGGGGATCACGACATGATTGTGTGCATCATCATCCAAAGAAATCCTTGGTATGAGTGTTCAAAATTTTATACGAATTTATTAGTATTCCTTGAAGCGAGACAGTGTATATAAAGTTGGCTAAATGATTGGAATTGTGATTGAAACATTTTTATTTATATACTTCTTTCCATTTTGCGGAACGTGTCGTCTTTTTCTTGAAACTTCCAAATCAAACTCTTCACCCC

mRNA sequence

GATCTATTTTTCGTTGAATTGATTATTATTTGCTACTTCATAGTGGGCTTGGAGGTATGCTAACTAGTTCGGCCCATATACATGTCCAGGAGCTCAACTGGGAGTTTGGGCCGATTCATAGTCGGCCTGTAATGAAAATATGTATTGAGCTCGGCCCAAATTCTCATTTAAATAGAATTGGCGGAGTGAAAATTGGGAGTTTTGATTTGATGAACGGAAACCAATAAGTAGATTGCCGCGTGGAGAAGAAACAGCGACTTGAAAAGAAAGGGAACTCCAAATCTTCGACCTCCGTTTTCTGGAGCGTCTGACAAACCCTTTCTACAATTCTACTTCAAATCTTTTCATTGAATCTTCCGATTCATTCTCCGTTTTGTGAATTCCTTTTGGATTCTCATCTTTCTGTTTCAATCTCTCTGGTTTCTCGATTTTTGAAGATCGAGAGAGGATTCCATTTGCTTTGCATCGACAATGTTTAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTTTGGCTACGTTAGAAAAGCTAAGCGAGACACTTGAAATGCTGGAGAAGAAAGAGAATGTTCTTGTGAAGAAGGCAGCTGCAGAGGTCGAAAGAGCAAAAGAATTCACAAGAGGGAAGAATAAACGAGCGGCTATACAATGTTTGAAGAGAAAAAGGCTTTATGAACAGCAAATAGAGCAGCTTGGAAATTTCCAATTGCGCATCCATGATCAAATGATTATGTTAGAAGGGGCAAAAGCTACAACGGAGACCGTAGATGCTCTACGAACAGGAGCATCTGCAATGAAGGCAATGCAGAAAGCAACGAATATTGATGATGTAGACAAGACGATGGATGAGATCAACGAGCAGACTGAGAATATGAAACAGATACAGGAAGCGTTATCAGCTCCAATTGGTGCTGCAGCTGATTTCGATGAGGATGAATTGGAGGCAGAGCTTGAAGATCTAGAAAGTGCGGAGTTGGAAGAGCAACTGCTGCAACCAGCATCAACAGCTCCTGCTGCACCTGTCTCAGTCCCGGGAGGCCGAGTTCCTGCACGCCCTGCTCCTCAAAAGAGAACTGCTGAAGAGGACGAACTCGCTGCTCTGCAAGCTGAGATGGCACTCTAAACAGGTAAAATGGGGATCACGACATGATTGTGTGCATCATCATCCAAAGAAATCCTTGGTATGAGTGTTCAAAATTTTATACGAATTTATTAGTATTCCTTGAAGCGAGACAGTGTATATAAAGTTGGCTAAATGATTGGAATTGTGATTGAAACATTTTTATTTATATACTTCTTTCCATTTTGCGGAACGTGTCGTCTTTTTCTTGAAACTTCCAAATCAAACTCTTCACCCC

Coding sequence (CDS)

ATGTTTAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTTTGGCTACGTTAGAAAAGCTAAGCGAGACACTTGAAATGCTGGAGAAGAAAGAGAATGTTCTTGTGAAGAAGGCAGCTGCAGAGGTCGAAAGAGCAAAAGAATTCACAAGAGGGAAGAATAAACGAGCGGCTATACAATGTTTGAAGAGAAAAAGGCTTTATGAACAGCAAATAGAGCAGCTTGGAAATTTCCAATTGCGCATCCATGATCAAATGATTATGTTAGAAGGGGCAAAAGCTACAACGGAGACCGTAGATGCTCTACGAACAGGAGCATCTGCAATGAAGGCAATGCAGAAAGCAACGAATATTGATGATGTAGACAAGACGATGGATGAGATCAACGAGCAGACTGAGAATATGAAACAGATACAGGAAGCGTTATCAGCTCCAATTGGTGCTGCAGCTGATTTCGATGAGGATGAATTGGAGGCAGAGCTTGAAGATCTAGAAAGTGCGGAGTTGGAAGAGCAACTGCTGCAACCAGCATCAACAGCTCCTGCTGCACCTGTCTCAGTCCCGGGAGGCCGAGTTCCTGCACGCCCTGCTCCTCAAAAGAGAACTGCTGAAGAGGACGAACTCGCTGCTCTGCAAGCTGAGATGGCACTCTAA

Protein sequence

MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAIQCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPASTAPAAPVSVPGGRVPARPAPQKRTAEEDELAALQAEMAL*
Homology
BLAST of CSPI02G00600 vs. ExPASy Swiss-Prot
Match: Q9SZE4 (Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=VPS32.2 PE=1 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 1.4e-92
Identity = 188/219 (85.84%), Postives = 202/219 (92.24%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60
           M NRLFGKPKQEANAL TL+KL+ETLEMLEKKE VL+KKA AEVE+AKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE Q+EQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEAL+ P+GAAADFDEDEL AEL++LES ELE QLLQPA+T
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 APAAP-VSVPGGRVPARPAPQKRTAEEDELAALQAEMAL 219
           AP  P V VP GR PARP PQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of CSPI02G00600 vs. ExPASy Swiss-Prot
Match: O82197 (Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VPS32.1 PE=1 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 2.8e-85
Identity = 181/217 (83.41%), Postives = 193/217 (88.94%), Query Frame = 0

Query: 3   NRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAIQC 62
           NRLFGKPKQE + L TL+KL+ETLEMLEKKENVL+KKA  EVE+AKEF+R KNKRAAIQC
Sbjct: 4   NRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQC 63

Query: 63  LKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 122
           LKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV
Sbjct: 64  LKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 123

Query: 123 DKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPASTAP 182
           DKTMDEINEQTENMKQIQEALSAP G A DFDEDELEAEL++LE AELEEQLLQP     
Sbjct: 124 DKTMDEINEQTENMKQIQEALSAPFG-ANDFDEDELEAELDELEGAELEEQLLQP----- 183

Query: 183 AAPVSVPGGRVPAR-PAPQKRTAEEDELAALQAEMAL 219
             P+ VP G  PAR PA ++ TAEEDELAALQAEMAL
Sbjct: 184 -VPIHVPQGNKPARAPAQKQPTAEEDELAALQAEMAL 213

BLAST of CSPI02G00600 vs. ExPASy Swiss-Prot
Match: Q7ZVC4 (Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 9.3e-28
Identity = 88/217 (40.55%), Postives = 127/217 (58.53%), Query Frame = 0

Query: 1   MFNRLF---GKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKR 60
           +F ++F   GK  + A+    +++L ET EML KK+  L KK   E+  AK+    KNKR
Sbjct: 3   LFGKMFGSGGKGGKSASPQEAIQRLRETEEMLTKKQEFLEKKIEQELVTAKK-NGTKNKR 62

Query: 61  AAIQCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKAT 120
           AA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA     
Sbjct: 63  AALQALKRKKRYEKQLAQIDGTLSTIEFQREALENAHTNTEVIKNMGYAAKAMKAAHDNM 122

Query: 121 NIDDVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQP 180
           +ID VD+ M +I EQ E  ++I +A+S P+G   +FDEDEL AELE+LE  EL++ LL+ 
Sbjct: 123 DIDKVDELMQDIIEQQELAQEISDAISKPVGFGEEFDEDELLAELEELEQEELDKNLLEI 182

Query: 181 ASTAPAAPVSVPGGRVPARPAPQKRTAEEDELAALQA 215
               P    +VP   +P+RPA +K   +ED++  L+A
Sbjct: 183 GDNVPLP--NVPSTSLPSRPAKKKEEEDEDDMKDLEA 216

BLAST of CSPI02G00600 vs. ExPASy Swiss-Prot
Match: Q6IQ73 (Charged multivesicular body protein 4c OS=Danio rerio OX=7955 GN=chmp4c PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 8.7e-26
Identity = 91/217 (41.94%), Postives = 122/217 (56.22%), Query Frame = 0

Query: 1   MFNRLF---GKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKR 60
           +F +LF   GK  +       ++KL ET EML KK++ L KK  AE+  AK+    KNKR
Sbjct: 3   VFGKLFGGGGKGGKGPTPQEAIQKLRETEEMLAKKQDFLEKKIDAELLIAKK-NGTKNKR 62

Query: 61  AAIQCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKAT 120
           AA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMK   +  
Sbjct: 63  AALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKTAHENM 122

Query: 121 NIDDVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQP 180
           +ID VD  M +I EQ E  ++I +A+S P+G   +FDEDEL AELE+LE  EL++ LLQ 
Sbjct: 123 DIDKVDDLMQDITEQQELAQEISDAISRPVGFGEEFDEDELMAELEELEQEELDKDLLQ- 182

Query: 181 ASTAPAAPVSVPGGRVPARPAP------QKRTAEEDE 209
                + P  VP   VP+ P P      QK+  EEDE
Sbjct: 183 ----ISGPEDVPLPNVPSNPLPKKTAVAQKKRQEEDE 213

BLAST of CSPI02G00600 vs. ExPASy Swiss-Prot
Match: Q5XGW6 (Charged multivesicular body protein 4b OS=Xenopus laevis OX=8355 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.9e-25
Identity = 80/207 (38.65%), Postives = 119/207 (57.49%), Query Frame = 0

Query: 7   GKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAIQCLKRK 66
           GK  +  +    ++KL +T EML KK+  L KK   E+  AK+    KNKRAA+Q LKRK
Sbjct: 12  GKGAKGPSPQEAIQKLRDTEEMLAKKQEFLEKKIEQELVTAKKHGT-KNKRAALQALKRK 71

Query: 67  RLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTM 126
           + YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA     +I+ VD+ M
Sbjct: 72  KRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKAAHDNMDIEKVDELM 131

Query: 127 DEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPASTAPAAPV 186
            +I +Q E  ++I +A+S P+G   DFDEDEL AELE+LE  EL++ LL+          
Sbjct: 132 QDIADQQELAQEISDAISKPVGFGEDFDEDELMAELEELEQEELDKNLLEVQGPETVPLP 191

Query: 187 SVPGGRVPARPAPQKRTAEEDELAALQ 214
           +VP   +PA+P  +K+  ++D++  L+
Sbjct: 192 NVPAASLPAKPVKKKQEEDDDDMRELE 217

BLAST of CSPI02G00600 vs. ExPASy TrEMBL
Match: A0A0A0LHT5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000620 PE=4 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 2.3e-106
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 APAAPVSVPGGRVPARPAPQKRTAEEDELAALQAEMAL 219
           APAAPVSVPGGRVPARPAPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPGGRVPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of CSPI02G00600 vs. ExPASy TrEMBL
Match: A0A1S3CAQ7 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucumis melo OX=3656 GN=LOC103498351 PE=4 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 1.3e-104
Identity = 216/218 (99.08%), Postives = 216/218 (99.08%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTR KNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 APAAPVSVPGGRVPARPAPQKRTAEEDELAALQAEMAL 219
           APAAPVSVP GRVPARPAPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of CSPI02G00600 vs. ExPASy TrEMBL
Match: A0A5A7V4V7 (Vacuolar protein sorting-associated protein 32-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00800 PE=4 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 1.3e-104
Identity = 216/218 (99.08%), Postives = 216/218 (99.08%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTR KNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 APAAPVSVPGGRVPARPAPQKRTAEEDELAALQAEMAL 219
           APAAPVSVP GRVPARPAPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of CSPI02G00600 vs. ExPASy TrEMBL
Match: A0A6J1G2S3 (vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita moschata OX=3662 GN=LOC111450291 PE=4 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 5.0e-101
Identity = 206/218 (94.50%), Postives = 212/218 (97.25%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKA+AEVE+AKEFTR KNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMD+INEQTENMKQIQEALS PIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDDINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAAPVSVPGGRVPARPAPQKRTAEEDELAALQAEMAL 219
            PAAPVS+P GRVPARP PQKRTAEEDEL ALQAEMAL
Sbjct: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELTALQAEMAL 218

BLAST of CSPI02G00600 vs. ExPASy TrEMBL
Match: A0A6J1FBK1 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita moschata OX=3662 GN=LOC111444128 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 8.5e-101
Identity = 206/218 (94.50%), Postives = 212/218 (97.25%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60
           M NRLFGKPKQEANALATL+KL+ETLEMLEKKENVL+KKAAAEVE+AKEFTR KNKRAAI
Sbjct: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAAPVSVPGGRVPARPAPQKRTAEEDELAALQAEMAL 219
           APA PVSVP GRVPARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 APAGPVSVPSGRVPARPVPQKRTAEEDELAALQAEMAL 218

BLAST of CSPI02G00600 vs. NCBI nr
Match: XP_004141038.1 (vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >XP_011648616.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >XP_031736822.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >KGN60554.1 hypothetical protein Csa_019460 [Cucumis sativus])

HSP 1 Score: 394.8 bits (1013), Expect = 4.8e-106
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 APAAPVSVPGGRVPARPAPQKRTAEEDELAALQAEMAL 219
           APAAPVSVPGGRVPARPAPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPGGRVPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of CSPI02G00600 vs. NCBI nr
Match: XP_008459153.1 (PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis melo] >XP_008459161.1 PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis melo] >XP_008459166.1 PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis melo] >XP_038889616.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Benincasa hispida] >XP_038889617.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Benincasa hispida] >KAA0061506.1 vacuolar protein sorting-associated protein 32-like protein 2 [Cucumis melo var. makuwa] >TYK10767.1 vacuolar protein sorting-associated protein 32-like protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 389.0 bits (998), Expect = 2.6e-104
Identity = 216/218 (99.08%), Postives = 216/218 (99.08%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTR KNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 APAAPVSVPGGRVPARPAPQKRTAEEDELAALQAEMAL 219
           APAAPVSVP GRVPARPAPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of CSPI02G00600 vs. NCBI nr
Match: KAG7030473.1 (Vacuolar protein sorting-associated protein 32-like 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 379.8 bits (974), Expect = 1.6e-101
Identity = 208/218 (95.41%), Postives = 213/218 (97.71%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKA+AEVE+AKEFTR KNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAAPVSVPGGRVPARPAPQKRTAEEDELAALQAEMAL 219
            PAAPVS+P GRVPARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELAALQAEMAL 218

BLAST of CSPI02G00600 vs. NCBI nr
Match: XP_023545615.1 (vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023545616.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023545619.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023545620.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547008.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547009.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547010.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547011.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 378.3 bits (970), Expect = 4.6e-101
Identity = 208/218 (95.41%), Postives = 212/218 (97.25%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKA+AEVE+AKEFTR KNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAAPVSVPGGRVPARPAPQKRTAEEDELAALQAEMAL 219
            PAAPVSVP GRVPARP PQKR AEEDELAALQAEMAL
Sbjct: 181 TPAAPVSVPSGRVPARPVPQKRNAEEDELAALQAEMAL 218

BLAST of CSPI02G00600 vs. NCBI nr
Match: XP_022946105.1 (vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022946106.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022946107.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022946108.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata])

HSP 1 Score: 377.1 bits (967), Expect = 1.0e-100
Identity = 206/218 (94.50%), Postives = 212/218 (97.25%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKA+AEVE+AKEFTR KNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMD+INEQTENMKQIQEALS PIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDDINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAAPVSVPGGRVPARPAPQKRTAEEDELAALQAEMAL 219
            PAAPVS+P GRVPARP PQKRTAEEDEL ALQAEMAL
Sbjct: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELTALQAEMAL 218

BLAST of CSPI02G00600 vs. TAIR 10
Match: AT4G29160.1 (SNF7 family protein )

HSP 1 Score: 340.5 bits (872), Expect = 1.0e-93
Identity = 188/219 (85.84%), Postives = 202/219 (92.24%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60
           M NRLFGKPKQEANAL TL+KL+ETLEMLEKKE VL+KKA AEVE+AKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE Q+EQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEAL+ P+GAAADFDEDEL AEL++LES ELE QLLQPA+T
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 APAAP-VSVPGGRVPARPAPQKRTAEEDELAALQAEMAL 219
           AP  P V VP GR PARP PQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of CSPI02G00600 vs. TAIR 10
Match: AT4G29160.3 (SNF7 family protein )

HSP 1 Score: 340.5 bits (872), Expect = 1.0e-93
Identity = 188/219 (85.84%), Postives = 202/219 (92.24%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60
           M NRLFGKPKQEANAL TL+KL+ETLEMLEKKE VL+KKA AEVE+AKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE Q+EQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEAL+ P+GAAADFDEDEL AEL++LES ELE QLLQPA+T
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 APAAP-VSVPGGRVPARPAPQKRTAEEDELAALQAEMAL 219
           AP  P V VP GR PARP PQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of CSPI02G00600 vs. TAIR 10
Match: AT2G19830.1 (SNF7 family protein )

HSP 1 Score: 316.2 bits (809), Expect = 2.0e-86
Identity = 181/217 (83.41%), Postives = 193/217 (88.94%), Query Frame = 0

Query: 3   NRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAIQC 62
           NRLFGKPKQE + L TL+KL+ETLEMLEKKENVL+KKA  EVE+AKEF+R KNKRAAIQC
Sbjct: 4   NRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQC 63

Query: 63  LKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 122
           LKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV
Sbjct: 64  LKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 123

Query: 123 DKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPASTAP 182
           DKTMDEINEQTENMKQIQEALSAP G A DFDEDELEAEL++LE AELEEQLLQP     
Sbjct: 124 DKTMDEINEQTENMKQIQEALSAPFG-ANDFDEDELEAELDELEGAELEEQLLQP----- 183

Query: 183 AAPVSVPGGRVPAR-PAPQKRTAEEDELAALQAEMAL 219
             P+ VP G  PAR PA ++ TAEEDELAALQAEMAL
Sbjct: 184 -VPIHVPQGNKPARAPAQKQPTAEEDELAALQAEMAL 213

BLAST of CSPI02G00600 vs. TAIR 10
Match: AT4G29160.2 (SNF7 family protein )

HSP 1 Score: 296.6 bits (758), Expect = 1.7e-80
Identity = 165/192 (85.94%), Postives = 177/192 (92.19%), Query Frame = 0

Query: 28  MLEKKENVLVKKAAAEVERAKEFTRGKNKRAAIQCLKRKRLYEQQIEQLGNFQLRIHDQM 87
           MLEKKE VL+KKA AEVE+AKE++R KNKRAAIQCLKRKRLYE Q+EQLGNFQLRIHDQM
Sbjct: 1   MLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAIQCLKRKRLYEGQVEQLGNFQLRIHDQM 60

Query: 88  IMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSAPI 147
           IMLEGAKATTETVDALR+GASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEAL+ P+
Sbjct: 61  IMLEGAKATTETVDALRSGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALATPM 120

Query: 148 GAAADFDEDELEAELEDLESAELEEQLLQPASTAPAAP-VSVPGGRVPARPAPQKRTAEE 207
           GAAADFDEDEL AEL++LES ELE QLLQPA+TAP  P V VP GR PARP PQKRTAEE
Sbjct: 121 GAAADFDEDELAAELDELESEELESQLLQPATTAPPLPSVPVPAGRQPARPVPQKRTAEE 180

Query: 208 DELAALQAEMAL 219
           +ELAALQAEMAL
Sbjct: 181 EELAALQAEMAL 192

BLAST of CSPI02G00600 vs. TAIR 10
Match: AT5G09260.1 (vacuolar protein sorting-associated protein 20.2 )

HSP 1 Score: 58.2 bits (139), Expect = 9.9e-09
Identity = 60/199 (30.15%), Postives = 96/199 (48.24%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60
           M N    KPK      A L  L      L + +  L K   AE + A++  R K K  A+
Sbjct: 1   MGNLFVKKPKITEVDRAIL-SLKTQRRKLGQYQQQLEKVIEAEKQAARDLIREKRKDRAL 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
             LK+KR  E+ ++Q+  + + +  Q+  +E         ++L+ G +A+KA+Q   N+D
Sbjct: 61  LALKKKRTQEELLKQVDQWLINVEQQLADIELTSKQKAVFESLKQGNNAIKAIQSEVNLD 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADF-DEDELEAELEDLESAELEEQLLQPAS 180
           DV K MD+    T   K  Q+ LSA +G      DE+E+ AE ++LES  + E + +  +
Sbjct: 121 DVQKLMDD----TAEAKAYQDELSAILGEKLSAEDEEEILAEFDNLESLLIVEDMPEVPT 180

Query: 181 T--APAAPVSVPGGRVPAR 197
           T   P  P  +    VP +
Sbjct: 181 TELMPEEPEKMDLPDVPTK 194

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZE41.4e-9285.84Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana... [more]
O821972.8e-8583.41Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana... [more]
Q7ZVC49.3e-2840.55Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=... [more]
Q6IQ738.7e-2641.94Charged multivesicular body protein 4c OS=Danio rerio OX=7955 GN=chmp4c PE=2 SV=... [more]
Q5XGW61.9e-2538.65Charged multivesicular body protein 4b OS=Xenopus laevis OX=8355 GN=chmp4b PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A0A0LHT52.3e-106100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000620 PE=4 SV=1[more]
A0A1S3CAQ71.3e-10499.08vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucumis melo OX=3656... [more]
A0A5A7V4V71.3e-10499.08Vacuolar protein sorting-associated protein 32-like protein 2 OS=Cucumis melo va... [more]
A0A6J1G2S35.0e-10194.50vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita mosch... [more]
A0A6J1FBK18.5e-10194.50vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita moschata O... [more]
Match NameE-valueIdentityDescription
XP_004141038.14.8e-106100.00vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >XP_0... [more]
XP_008459153.12.6e-10499.08PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis mel... [more]
KAG7030473.11.6e-10195.41Vacuolar protein sorting-associated protein 32-like 2 [Cucurbita argyrosperma su... [more]
XP_023545615.14.6e-10195.41vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo su... [more]
XP_022946105.11.0e-10094.50vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschat... [more]
Match NameE-valueIdentityDescription
AT4G29160.11.0e-9385.84SNF7 family protein [more]
AT4G29160.31.0e-9385.84SNF7 family protein [more]
AT2G19830.12.0e-8683.41SNF7 family protein [more]
AT4G29160.21.7e-8085.94SNF7 family protein [more]
AT5G09260.19.9e-0930.15vacuolar protein sorting-associated protein 20.2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 151..175
NoneNo IPR availableCOILSCoilCoilcoord: 122..142
NoneNo IPR availableCOILSCoilCoilcoord: 12..39
NoneNo IPR availableGENE3D1.10.287.1060coord: 14..91
e-value: 2.5E-22
score: 80.8
NoneNo IPR availableGENE3D6.10.250.1710coord: 117..172
e-value: 2.4E-8
score: 35.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 174..218
NoneNo IPR availablePANTHERPTHR22761CHARGED MULTIVESICULAR BODY PROTEINcoord: 1..218
NoneNo IPR availablePANTHERPTHR22761:SF63VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 32 HOMOLOG 1coord: 1..218
IPR005024Snf7 familyPFAMPF03357Snf7coord: 20..185
e-value: 4.4E-46
score: 156.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G00600.1CSPI02G00600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway
biological_process GO:0006900 vesicle budding from membrane
biological_process GO:0007034 vacuolar transport
cellular_component GO:0009898 cytoplasmic side of plasma membrane
cellular_component GO:0000815 ESCRT III complex
cellular_component GO:0005771 multivesicular body