CSPI02G00240 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G00240
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	thermospermine synthase ACAULIS5-like
Location	Chr2: 161182 .. 163385 (+)
RNA-Seq Expression	CSPI02G00240
Synteny	CSPI02G00240

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACTTTTCTTCTTCACAATATTCACTTTCACTTCAATTTTCCACAATGTTTTCTTCCTTCTGCTCTGCCCTTTCAGTTCTCTTTGTTCCCAACTCCTCTTCTCTCCCTCTTGATCATATTCAACAACTCCCTCAATGGTTCGAGGAAGAACTCGAGGATGATCTCAAATGGTCCTTTGCCGTCAACAGGTCCATTTCTTGTTTCCTTTTGTTCTCGTGTTCATAAGATGTTTGTATTCTTTCCTTTAAAACAATTATTTCTTTGTAGAGTGGATGCAAAATCTGAGCGTATTTTTTAAGTTAAAATTGATACTGACGTTTTTGACTTTCTTTAGGGTACTTCATGCAACAACTAGCGAGTTCCAAGATATAGTTCTGTTGGATACCAAGCGGTTTGGGAAGGTTTGCATTATCCCACATTAGTTTCAGTTTCCTTCCCAATAACCCCTTGATTAATTTAAATGCAAAATTTCTGAAATTTTGTTGACATCGCTTTCAGGCTCTATTGCTTGATGGGAAGCTGCAAAGTGCTGAAAAAGATGAATTCATCTATCATGAGTCCTTGGTTCATCCTGCTCTGCTACTGCATCATAAGTATATGCTCTGTTCATATTTGGTTTAGTTCTTTTAAGTAATAAGATGGTTTTTGTGGGACTGCAATCTGAAGCGTAGCTGATTTTTCTTCTGATCTTTCCTTTTGATTTTCTGAATGAATGATTTTCAGTCCCAAAACAGTGTTTATAATGGGAGGTGGAGAAGGGTGTACTGCTCGAGAGACACTAAAGCATAAAAGTATTGAGAAAGTTGTCATGTGCGATATTGATAGGGTATGGTTCTCAAAATCCTAAGATTCACTCGTTTGTAAATTCGTGTGGCAAAAGGATCGAGGAAGTTTAATTTTATGTCTTGAAAAAGTATTTAGTAGAGATACTTCAATGGAAGGAAAAGAGAAAAAAAATGAGAAACACTAGTTTTTGCATGAAGTTTTTAATTTACATTGTAGGATGTTGTCAATTTCTGCCGCGCACATCTAAAAGAAAACCAAGATGCATTTCAGGACGAGAGGCTTCATATTATTTTTGACGATGCAAAGTGAGTAGCTTTTGCCCTTTGGACTGAATCCGCCATTTGTATTTTAGTTATGCAGTTTGGTGAAGCTTGTTTAAACTATATCTCAACTTTTGCTTGAATCAATGCACATTTCAAACGAATTCTAGTTTAGATTTGAAAGAACTAACCTGAGATTGAAAAAGGGCTGGATTGGAGGGACGTCCAGAGAAGTTCGATGTAATAATTGGAGATTTATCAGATCCACATGAAGGAGGACCATGCAATCATCTGTACACTAAGTCCTTCTATGAGGACGTCATTAAGCCCAAGCTCAGTGACAATGGTATATTTGTTACTCAGGTACAGTATATAACATATTCATAACCCTCTGCTATTGTTCCGGGTTAGTTACTACTTTTACTAAGATTTAATACACTGTTACAAAAATGCAGGCCGGCCCTGCTGGTATTCTCTCCCACAAGGTCTTCTCATCAATATACAACACCGTAAAGCATGTCTTCAGATGTAAGACGTTGTTAACATAATCCCAACATGCTATTGTGTGTTTTGCCTGAACATTCCATTCATGTATTTGTTAAGATAATTTTCAAGTGCTGTTTTGATTACACATACAGCTCATGTTTGGCATGCCATCCTTTGTTTTATGTGCACAGATGTGATTGCATATACCGCTCATGTTCCATCCTATGCTGATTCATGTGGATGGGTTTTGGTAAGCAACTACAAAGAATTCCTTATGTATCTTATTGCCCGAAATGAAAAAGAAAGAGACTGACACATATAGAATGATTTGCAACGGTAGGCTTCTGATCATCCTATTAAACTGGACATCGAGGATCTCAACAATAAGATTCGTGAGAGAGTCCAAGGTGAGCTACACTACTTGGATGGTGCATTCATCGTATCGTCAACAGTCATAAACAAAACCATCCGTACATTGTAAGTATAAAGATAGTTGATCTCTCTCCTCTTCCTTTGCTGAGAAGGGGAAAAATCACGTATCTTATTGAGTGACCAATAATTTTTGGATCATGCGTTTGTTGTGGTTGTGTGCAGGATGATGAATGAGACTCATGTGTTCACTGAGGAAGATGCAAGGTTCGCACATGGGCGAGGGCTGGTTGCCAATGCCTAG

mRNA sequence

ACTTTTCTTCTTCACAATATTCACTTTCACTTCAATTTTCCACAATGTTTTCTTCCTTCTGCTCTGCCCTTTCAGTTCTCTTTGTTCCCAACTCCTCTTCTCTCCCTCTTGATCATATTCAACAACTCCCTCAATGGTTCGAGGAAGAACTCGAGGATGATCTCAAATGGTCCTTTGCCGTCAACAGGGTACTTCATGCAACAACTAGCGAGTTCCAAGATATAGTTCTGTTGGATACCAAGCGGTTTGGGAAGGCTCTATTGCTTGATGGGAAGCTGCAAAGTGCTGAAAAAGATGAATTCATCTATCATGAGTCCTTGGTTCATCCTGCTCTGCTACTGCATCATAATCCCAAAACAGTGTTTATAATGGGAGGTGGAGAAGGGTGTACTGCTCGAGAGACACTAAAGCATAAAAGTATTGAGAAAGTTGTCATGTGCGATATTGATAGGGATGTTGTCAATTTCTGCCGCGCACATCTAAAAGAAAACCAAGATGCATTTCAGGACGAGAGGCTTCATATTATTTTTGACGATGCAAAGGCTGGATTGGAGGGACGTCCAGAGAAGTTCGATGTAATAATTGGAGATTTATCAGATCCACATGAAGGAGGACCATGCAATCATCTGTACACTAAGTCCTTCTATGAGGACGTCATTAAGCCCAAGCTCAGTGACAATGGTATATTTGTTACTCAGGCCGGCCCTGCTGGTATTCTCTCCCACAAGGTCTTCTCATCAATATACAACACCGTAAAGCATGTCTTCAGATATGTGATTGCATATACCGCTCATGTTCCATCCTATGCTGATTCATGTGGATGGGTTTTGGCTTCTGATCATCCTATTAAACTGGACATCGAGGATCTCAACAATAAGATTCGTGAGAGAGTCCAAGGTGAGCTACACTACTTGGATGGTGCATTCATCGTATCGTCAACAGTCATAAACAAAACCATCCGTACATTGATGATGAATGAGACTCATGTGTTCACTGAGGAAGATGCAAGGTTCGCACATGGGCGAGGGCTGGTTGCCAATGCCTAG

Coding sequence (CDS)

ATGTTTTCTTCCTTCTGCTCTGCCCTTTCAGTTCTCTTTGTTCCCAACTCCTCTTCTCTCCCTCTTGATCATATTCAACAACTCCCTCAATGGTTCGAGGAAGAACTCGAGGATGATCTCAAATGGTCCTTTGCCGTCAACAGGGTACTTCATGCAACAACTAGCGAGTTCCAAGATATAGTTCTGTTGGATACCAAGCGGTTTGGGAAGGCTCTATTGCTTGATGGGAAGCTGCAAAGTGCTGAAAAAGATGAATTCATCTATCATGAGTCCTTGGTTCATCCTGCTCTGCTACTGCATCATAATCCCAAAACAGTGTTTATAATGGGAGGTGGAGAAGGGTGTACTGCTCGAGAGACACTAAAGCATAAAAGTATTGAGAAAGTTGTCATGTGCGATATTGATAGGGATGTTGTCAATTTCTGCCGCGCACATCTAAAAGAAAACCAAGATGCATTTCAGGACGAGAGGCTTCATATTATTTTTGACGATGCAAAGGCTGGATTGGAGGGACGTCCAGAGAAGTTCGATGTAATAATTGGAGATTTATCAGATCCACATGAAGGAGGACCATGCAATCATCTGTACACTAAGTCCTTCTATGAGGACGTCATTAAGCCCAAGCTCAGTGACAATGGTATATTTGTTACTCAGGCCGGCCCTGCTGGTATTCTCTCCCACAAGGTCTTCTCATCAATATACAACACCGTAAAGCATGTCTTCAGATATGTGATTGCATATACCGCTCATGTTCCATCCTATGCTGATTCATGTGGATGGGTTTTGGCTTCTGATCATCCTATTAAACTGGACATCGAGGATCTCAACAATAAGATTCGTGAGAGAGTCCAAGGTGAGCTACACTACTTGGATGGTGCATTCATCGTATCGTCAACAGTCATAAACAAAACCATCCGTACATTGATGATGAATGAGACTCATGTGTTCACTGAGGAAGATGCAAGGTTCGCACATGGGCGAGGGCTGGTTGCCAATGCCTAG

Protein sequence

MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARETLKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVIIGDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTVINKTIRTLMMNETHVFTEEDARFAHGRGLVANA*

Homology

[Close]

BLAST of CSPI02G00240 vs. ExPASy Swiss-Prot
Match: Q9S7X6 (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 7.8e-119
Identity = 198/305 (64.92%), Postives = 247/305 (80.98%), Query Frame = 0


Query: 26  QQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDIVLLDTKRFGKALLLDGKLQSAEKDE 85
           QQ   W+EE ++DDLKWSFA+N VLH  TSE+QDI LLDTKRFGK L++DGK+QSAE+DE
Sbjct: 30  QQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGKMQSAERDE 89

Query: 86  FIYHESLVHPALLLHHNPKTVFIMGGGEGCTARETLKHKSIEKVVMCDIDRDVVNFCRAH 145
           FIYHE L+HPALL H NPKTVFIMGGGEG  ARE LKH +IEKVVMCDID++VV+FCR  
Sbjct: 90  FIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQEVVDFCRRF 149

Query: 146 LKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVIIGDLSDPHEGGPCNHLYTKSFYEDVI 205
           L  N DAF +++L ++  DAKA LE R EKFD+I+GDL+DP EGGPC  LYTKSFY++++
Sbjct: 150 LTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLYTKSFYQNIL 209

Query: 206 KPKLSDNGIFVTQAGPAGILSHK-VFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLAS 265
           KPKLS NGIFVTQAGPAGI +HK VF+SIYNT+K VF+YV AYTAHVPS+AD+ GWV+AS
Sbjct: 210 KPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFADTWGWVMAS 269

Query: 266 DHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTVINKTIRTLMMNETHVFTEEDARFA 325
           DH   +++++++ +I ERV GEL YL+    VS+  +NKTI   +  ET V++EE+ARF 
Sbjct: 270 DHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEVYSEENARFI 329

Query: 326 HGRGL 330
           HG G+
Sbjct: 330 HGHGV 334

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BLAST of CSPI02G00240 vs. ExPASy Swiss-Prot
Match: Q975S5 (Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=speE PE=3 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.5e-58
Identity = 117/279 (41.94%), Postives = 176/279 (63.08%), Query Frame = 0


Query: 45  AVNRVLHATTSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPK 104
           A+ +V+    + +Q  +L++  RFGKAL+LDGK+QS   DEFIYHE+LVHP LL  +NP+
Sbjct: 20  AITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDEFIYHEALVHPLLLSINNPE 79

Query: 105 TVFIMGGGEGCTARETLKHKSIEKVVMCDIDRDVVNFCRAHLKE-NQDAFQDERLHIIFD 164
            + I+GGGEG T RE LKHK+I+ V M DID  V++F + +L+E +Q AF + +  ++ +
Sbjct: 80  KILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKYLQEWHQGAFDNPKSKLVIE 139

Query: 165 DAKAGLEGRPEKFDVIIGDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAG 224
           D    ++   EKFD ++ DL+DP +  P   LYTK FYE+V   ++S  GI      P+ 
Sbjct: 140 DGYKFIKETKEKFDAVVIDLTDPIKDSPSQMLYTKEFYEEV--KRISKWGIVTQATSPS- 199

Query: 225 ILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLASD--HPIKLDIEDLNNKIRE 284
             S + FS IYNT+KHVF+ V A   +VP++    G+V ASD  +P +   E++NN+I+E
Sbjct: 200 -FSLETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEVNPAEFSKEEINNRIKE 259

Query: 285 RVQGELHYLDGAFIVSSTVINKTIRTLMMNETHVFTEED 321
           R+ G L + DG   ++   I K IR ++  E  + T E+
Sbjct: 260 RIDGSLRFYDGETHITMFSIPKHIREVLEKENKISTREN 294

[Close]

BLAST of CSPI02G00240 vs. ExPASy Swiss-Prot
Match: Q72K55 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=speE PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.4e-54
Identity = 116/287 (40.42%), Postives = 171/287 (59.58%), Query Frame = 0


Query: 46  VNRVLHATTSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKT 105
           + RV+ +  + FQD  L ++K FGK L+LD  +QS E+DE+IYHE+LVHPA+L H  PK 
Sbjct: 21  MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80

Query: 106 VFIMGGGEGCTARETLKHKSIEKVVMCDIDRDVVNFCRAHLKE-NQDAFQDERLHIIFDD 165
           V I+GGGEG T RE LKH ++EK VM DID ++V   + H+ E +Q AF D R  ++ DD
Sbjct: 81  VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140

Query: 166 AKAGLEGRPEKFDVIIGDLSDP-HEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAG 225
           A+A LE   E++DV+I DL+DP  E  P   LYT  FY  ++K  L+  G+   QAG   
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFYR-LVKAHLNPGGVMGMQAGMIL 200

Query: 226 ILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLASD--HPIKLDIEDLNNKIRE 285
           +  H+V   ++ TV+  FRYV +Y  H+P +  + G++LASD   P       +  +IRE
Sbjct: 201 LTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLNFGFLLASDAFDPAAFSEGVIEARIRE 260

Query: 286 RVQGELHYLDGAFIVSSTVINKTIRTLMMNETHVFTEEDARFAHGRG 329
           R    L +L   ++ +  V+ K +   +  ET V T+++  +    G
Sbjct: 261 R-NLALRHLTAPYLEAMFVLPKDLLEALEKETMVSTDQNPFYVTPEG 305

[Close]

BLAST of CSPI02G00240 vs. ExPASy Swiss-Prot
Match: Q5SK28 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=speE PE=1 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.4e-54
Identity = 116/287 (40.42%), Postives = 171/287 (59.58%), Query Frame = 0


Query: 46  VNRVLHATTSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKT 105
           + RV+ +  + FQD  L ++K FGK L+LD  +QS E+DE+IYHE+LVHPA+L H  PK 
Sbjct: 21  MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80

Query: 106 VFIMGGGEGCTARETLKHKSIEKVVMCDIDRDVVNFCRAHLKE-NQDAFQDERLHIIFDD 165
           V I+GGGEG T RE LKH ++EK VM DID ++V   + H+ E +Q AF D R  ++ DD
Sbjct: 81  VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140

Query: 166 AKAGLEGRPEKFDVIIGDLSDP-HEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAG 225
           A+A LE   E++DV+I DL+DP  E  P   LYT  FY  ++K  L+  G+   QAG   
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFYR-LVKAHLNPGGVMGMQAGMIL 200

Query: 226 ILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLASD--HPIKLDIEDLNNKIRE 285
           +  H+V   ++ TV+  FRYV +Y  H+P +  + G++LASD   P       +  +IRE
Sbjct: 201 LTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLNFGFLLASDAFDPAAFSEGVIEARIRE 260

Query: 286 RVQGELHYLDGAFIVSSTVINKTIRTLMMNETHVFTEEDARFAHGRG 329
           R    L +L   ++ +  V+ K +   +  ET V T+++  +    G
Sbjct: 261 R-NLALRHLTAPYLEAMFVLPKDLLEALEKETMVSTDQNPFYVTPEG 305

[Close]

BLAST of CSPI02G00240 vs. ExPASy Swiss-Prot
Match: Q9YE02 (Polyamine aminopropyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=speE PE=3 SV=2)

HSP 1 Score: 214.2 bits (544), Expect = 2.3e-54
Identity = 122/299 (40.80%), Postives = 175/299 (58.53%), Query Frame = 0


Query: 31  WFEEELEDDLKWSFAVNRVLHATTSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHE 90
           WF E L  D      +  V+    S FQ+I ++     GK L+LDGK QS+E DEF+YHE
Sbjct: 15  WFLEWLTPDRATLKHIEDVIFQGRSRFQEIAVVRVSGEGKVLVLDGKTQSSESDEFMYHE 74

Query: 91  SLVHPALLLHHNPKTVFIMGGGEGCTARETLKHKSIEKVVMCDIDRDVVNFCRAHLKE-N 150
           +LVHPA++LH +P+ V I+GGGEG T RE LKH+S+EK VM DID  VVN  R HL+E +
Sbjct: 75  ALVHPAMILHGSPRKVLILGGGEGATLREVLKHRSVEKAVMVDIDETVVNVAREHLREWH 134

Query: 151 QDAFQDERLHIIFDDAKAGLEGRPEK-FDVIIGDLSDPHEGGPCNHLYTKSFYEDVIKPK 210
           + AF D R  ++ DDA   +  + E  FDV+I DL DP E GP   LY++ +Y  ++K  
Sbjct: 135 RGAFDDPRAEVVIDDAWNYVASKAETGFDVVIADLVDPLEAGPATRLYSEEYYR-MVKDV 194

Query: 211 LSDNGIFVTQAGPAGILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLASDHPI 270
           ++  G+FVTQA     L+ +  + I NTV  VF++V +Y  +VPS+    G+V+ASD   
Sbjct: 195 MNPGGVFVTQAVSISHLT-EYHAIIRNTVARVFKHVESYGVYVPSFDSMWGFVVASDDKD 254

Query: 271 KLDIED---LNNKIRERVQG-ELHYLDGAFIVSSTVINKTIRTLMMNETHVFTEEDARF 324
              + D      ++  ++QG EL +LD A ++    I K  R  +  E    T ++  F
Sbjct: 255 PRILGDRGFFETRLSHQLQGAELRFLDYASMLHMLNIPKMYREAIAREKRYATLDNQVF 311

[Close]

BLAST of CSPI02G00240 vs. ExPASy TrEMBL
Match: A0A0A0LET5 (PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000240 PE=3 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 2.6e-194
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 0


Query: 1   MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60
           MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI
Sbjct: 1   MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120

Query: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII 180
           LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV 300
           FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV
Sbjct: 241 FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV 300

Query: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVANA 334
           INKTIRTLMMNETHVFTEEDARFAHGRGLVANA
Sbjct: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVANA 333

[Close]

BLAST of CSPI02G00240 vs. ExPASy TrEMBL
Match: A0A5A7UZV1 (Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G001170 PE=3 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 3.1e-187
Identity = 321/333 (96.40%), Postives = 326/333 (97.90%), Query Frame = 0


Query: 1   MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60
           MFSSFCSALSVLF+PNSSSLPLDHI QLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI
Sbjct: 1   MFSSFCSALSVLFIPNSSSLPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120

Query: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII 180
           LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDE+LHIIFDDAKAGLEGR EKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV 300
           F+YVIAYTAHVPSYADSCGWVLASDHPI+LD EDLNNKI ERV+GEL YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHVPSYADSCGWVLASDHPIRLDKEDLNNKIHERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVANA 334
           INKTIRTLMMNETHVFTEEDARFAHGRGLV NA
Sbjct: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVGNA 333

[Close]

BLAST of CSPI02G00240 vs. ExPASy TrEMBL
Match: A0A1S3CAR3 (thermospermine synthase ACAULIS5-like OS=Cucumis melo OX=3656 GN=LOC103498720 PE=3 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 3.1e-187
Identity = 321/333 (96.40%), Postives = 326/333 (97.90%), Query Frame = 0


Query: 1   MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60
           MFSSFCSALSVLF+PNSSSLPLDHI QLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI
Sbjct: 1   MFSSFCSALSVLFIPNSSSLPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120

Query: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII 180
           LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDE+LHIIFDDAKAGLEGR EKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV 300
           F+YVIAYTAHVPSYADSCGWVLASDHPI+LD EDLNNKI ERV+GEL YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHVPSYADSCGWVLASDHPIRLDKEDLNNKIHERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVANA 334
           INKTIRTLMMNETHVFTEEDARFAHGRGLV NA
Sbjct: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVGNA 333

[Close]

BLAST of CSPI02G00240 vs. ExPASy TrEMBL
Match: A0A6J1G4J8 (thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111450716 PE=3 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 1.3e-164
Identity = 280/333 (84.08%), Postives = 305/333 (91.59%), Query Frame = 0


Query: 1   MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60
           M S  CSAL  L +P S+S  + H+    +WFEE+LEDDLKWSFAVNR+LHA TS+FQDI
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVPHVS---EWFEEQLEDDLKWSFAVNRILHAATSKFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHHNPKTVFI+GGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARET 120

Query: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII 180
           LKHKSIEKVVMCDIDRDVVNFCRAHL ENQDAFQDE+LHIIFDDAK GLEG+PEKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
            DL+DPHEGGPCNHLYTKSFYE+ IKPKL+DNGIFVTQAGP+GILS+ VFSSIYNTVKHV
Sbjct: 181 SDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV 300
           F+YVIAYTAH+PSYADS GWVLASDHP KLDIEDL+ +IRERV+GEL YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHIPSYADSSGWVLASDHPFKLDIEDLDKRIRERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVANA 334
           INKTIRT MMNETHVFTEEDARF HG+GL ++A
Sbjct: 301 INKTIRTAMMNETHVFTEEDARFTHGQGLASHA 330

[Close]

BLAST of CSPI02G00240 vs. ExPASy TrEMBL
Match: A0A6J1KJ05 (thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC111493662 PE=3 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 4.5e-162
Identity = 276/333 (82.88%), Postives = 304/333 (91.29%), Query Frame = 0


Query: 1   MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60
           M S  CSAL  L +P S+S     + Q+ +WFEE+LEDDLKWSFAV R+LHA TS+FQDI
Sbjct: 1   MLSPLCSALPALSIPTSNSTA---VPQVSEWFEEQLEDDLKWSFAVTRILHAATSKFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHHNPKTVFI+GGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARET 120

Query: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII 180
           LKHKSIEKVVMCDIDRDVVNFCRAHL ENQDAFQDE+LHIIF+DAK GLEG+PEKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFNDAKVGLEGQPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
            DL+DPHEGGPCNHLYTKSFYE+ IKPKL+DNGIFVTQAGP+GILS+ VFSSIYNTVKHV
Sbjct: 181 SDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV 300
           F+YVIAYTAH+PSYADS GWVLASDHP +LD+EDL+ +IRERV+GEL YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHIPSYADSSGWVLASDHPFELDMEDLDKRIRERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVANA 334
           INKTIRT MMNETHVFTEEDARF HG+GL ++A
Sbjct: 301 INKTIRTAMMNETHVFTEEDARFTHGQGLASHA 330

[Close]

BLAST of CSPI02G00240 vs. NCBI nr
Match: XP_004141174.1 (thermospermine synthase ACAULIS5 [Cucumis sativus] >KGN60515.1 hypothetical protein Csa_019446 [Cucumis sativus])

HSP 1 Score: 687.6 bits (1773), Expect = 5.5e-194
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 0


Query: 1   MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60
           MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI
Sbjct: 1   MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120

Query: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII 180
           LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV 300
           FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV
Sbjct: 241 FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV 300

Query: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVANA 334
           INKTIRTLMMNETHVFTEEDARFAHGRGLVANA
Sbjct: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVANA 333

[Close]

BLAST of CSPI02G00240 vs. NCBI nr
Match: XP_008459675.1 (PREDICTED: thermospermine synthase ACAULIS5-like [Cucumis melo] >KAA0061473.1 thermospermine synthase ACAULIS5-like [Cucumis melo var. makuwa] >TYK10801.1 thermospermine synthase ACAULIS5-like [Cucumis melo var. makuwa])

HSP 1 Score: 664.1 bits (1712), Expect = 6.5e-187
Identity = 321/333 (96.40%), Postives = 326/333 (97.90%), Query Frame = 0


Query: 1   MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60
           MFSSFCSALSVLF+PNSSSLPLDHI QLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI
Sbjct: 1   MFSSFCSALSVLFIPNSSSLPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120

Query: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII 180
           LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDE+LHIIFDDAKAGLEGR EKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV 300
           F+YVIAYTAHVPSYADSCGWVLASDHPI+LD EDLNNKI ERV+GEL YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHVPSYADSCGWVLASDHPIRLDKEDLNNKIHERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVANA 334
           INKTIRTLMMNETHVFTEEDARFAHGRGLV NA
Sbjct: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVGNA 333

[Close]

BLAST of CSPI02G00240 vs. NCBI nr
Match: XP_038890496.1 (thermospermine synthase ACAULIS5-like [Benincasa hispida])

HSP 1 Score: 646.0 bits (1665), Expect = 1.8e-181
Identity = 305/333 (91.59%), Postives = 325/333 (97.60%), Query Frame = 0


Query: 1   MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60
           MFSSFCSALSVLF+PNS+SLPLDHI Q+ +WFEE+LEDDLKWSFAVNR+LHA TSEFQDI
Sbjct: 1   MFSSFCSALSVLFIPNSTSLPLDHIHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120

Query: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII 180
           LKHK IEKVVMCDIDRDVVNFCRAHLKENQDAFQDE+LHIIFDDAKAGLEGRPEKFDVII
Sbjct: 121 LKHKDIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYE+VIKPKL+DNGIFVTQAGPAGILSH+VFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHEVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV 300
           F+YVIAYTAHVPSYADSCGWVLA DHPI+LD+EDLNN+IRERV+GELHYLDGA+IVSSTV
Sbjct: 241 FKYVIAYTAHVPSYADSCGWVLACDHPIELDMEDLNNRIRERVRGELHYLDGAYIVSSTV 300

Query: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVANA 334
           +NKTIR  MMNETH+FTEEDARFAHGRGLV++A
Sbjct: 301 VNKTIRASMMNETHLFTEEDARFAHGRGLVSDA 333

[Close]

BLAST of CSPI02G00240 vs. NCBI nr
Match: XP_022946737.1 (thermospermine synthase ACAULIS5-like [Cucurbita moschata])

HSP 1 Score: 589.0 bits (1517), Expect = 2.6e-164
Identity = 280/333 (84.08%), Postives = 305/333 (91.59%), Query Frame = 0


Query: 1   MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60
           M S  CSAL  L +P S+S  + H+    +WFEE+LEDDLKWSFAVNR+LHA TS+FQDI
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVPHVS---EWFEEQLEDDLKWSFAVNRILHAATSKFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHHNPKTVFI+GGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARET 120

Query: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII 180
           LKHKSIEKVVMCDIDRDVVNFCRAHL ENQDAFQDE+LHIIFDDAK GLEG+PEKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
            DL+DPHEGGPCNHLYTKSFYE+ IKPKL+DNGIFVTQAGP+GILS+ VFSSIYNTVKHV
Sbjct: 181 SDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV 300
           F+YVIAYTAH+PSYADS GWVLASDHP KLDIEDL+ +IRERV+GEL YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHIPSYADSSGWVLASDHPFKLDIEDLDKRIRERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVANA 334
           INKTIRT MMNETHVFTEEDARF HG+GL ++A
Sbjct: 301 INKTIRTAMMNETHVFTEEDARFTHGQGLASHA 330

[Close]

BLAST of CSPI02G00240 vs. NCBI nr
Match: KAG7030499.1 (Thermospermine synthase ACAULIS5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 588.2 bits (1515), Expect = 4.5e-164
Identity = 278/333 (83.48%), Postives = 305/333 (91.59%), Query Frame = 0


Query: 1   MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60
           M S  CSAL  L +P S+S  + H+    +WFEE+LEDDLKWSFAVNR+LHA TS+FQDI
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVPHVS---EWFEEQLEDDLKWSFAVNRILHAATSKFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHHNPKTVFI+GGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARET 120

Query: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII 180
           LKHKSIEKVVMCDIDRDVVNFCRAHL ENQDAFQDE+LHIIFDDAK GLEG+PEKFD+II
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
            DL+DPHEGGPCNHLYTKSFYE+ IKPKL+DNGIFVTQAGP+GILS+ VFSSIYNTVKHV
Sbjct: 181 SDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV 300
           F+YVIAYTAH+PSYADS GWVLASDHP KLD+EDL+ +IRERV+GEL YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVANA 334
           INKTIRT MMNETHVFTEEDARF HG+GL ++A
Sbjct: 301 INKTIRTAMMNETHVFTEEDARFTHGQGLASHA 330

[Close]

BLAST of CSPI02G00240 vs. TAIR 10
Match: AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 428.3 bits (1100), Expect = 5.6e-120
Identity = 198/305 (64.92%), Postives = 247/305 (80.98%), Query Frame = 0


Query: 26  QQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDIVLLDTKRFGKALLLDGKLQSAEKDE 85
           QQ   W+EE ++DDLKWSFA+N VLH  TSE+QDI LLDTKRFGK L++DGK+QSAE+DE
Sbjct: 30  QQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGKMQSAERDE 89

Query: 86  FIYHESLVHPALLLHHNPKTVFIMGGGEGCTARETLKHKSIEKVVMCDIDRDVVNFCRAH 145
           FIYHE L+HPALL H NPKTVFIMGGGEG  ARE LKH +IEKVVMCDID++VV+FCR  
Sbjct: 90  FIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQEVVDFCRRF 149

Query: 146 LKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVIIGDLSDPHEGGPCNHLYTKSFYEDVI 205
           L  N DAF +++L ++  DAKA LE R EKFD+I+GDL+DP EGGPC  LYTKSFY++++
Sbjct: 150 LTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLYTKSFYQNIL 209

Query: 206 KPKLSDNGIFVTQAGPAGILSHK-VFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLAS 265
           KPKLS NGIFVTQAGPAGI +HK VF+SIYNT+K VF+YV AYTAHVPS+AD+ GWV+AS
Sbjct: 210 KPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFADTWGWVMAS 269

Query: 266 DHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTVINKTIRTLMMNETHVFTEEDARFA 325
           DH   +++++++ +I ERV GEL YL+    VS+  +NKTI   +  ET V++EE+ARF 
Sbjct: 270 DHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEVYSEENARFI 329

Query: 326 HGRGL 330
           HG G+
Sbjct: 330 HGHGV 334

[Close]

BLAST of CSPI02G00240 vs. TAIR 10
Match: AT5G19530.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 428.3 bits (1100), Expect = 5.6e-120
Identity = 198/305 (64.92%), Postives = 247/305 (80.98%), Query Frame = 0


Query: 26  QQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDIVLLDTKRFGKALLLDGKLQSAEKDE 85
           QQ   W+EE ++DDLKWSFA+N VLH  TSE+QDI LLDTKRFGK L++DGK+QSAE+DE
Sbjct: 23  QQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGKMQSAERDE 82

Query: 86  FIYHESLVHPALLLHHNPKTVFIMGGGEGCTARETLKHKSIEKVVMCDIDRDVVNFCRAH 145
           FIYHE L+HPALL H NPKTVFIMGGGEG  ARE LKH +IEKVVMCDID++VV+FCR  
Sbjct: 83  FIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQEVVDFCRRF 142

Query: 146 LKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVIIGDLSDPHEGGPCNHLYTKSFYEDVI 205
           L  N DAF +++L ++  DAKA LE R EKFD+I+GDL+DP EGGPC  LYTKSFY++++
Sbjct: 143 LTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLYTKSFYQNIL 202

Query: 206 KPKLSDNGIFVTQAGPAGILSHK-VFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLAS 265
           KPKLS NGIFVTQAGPAGI +HK VF+SIYNT+K VF+YV AYTAHVPS+AD+ GWV+AS
Sbjct: 203 KPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFADTWGWVMAS 262

Query: 266 DHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTVINKTIRTLMMNETHVFTEEDARFA 325
           DH   +++++++ +I ERV GEL YL+    VS+  +NKTI   +  ET V++EE+ARF 
Sbjct: 263 DHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEVYSEENARFI 322

Query: 326 HGRGL 330
           HG G+
Sbjct: 323 HGHGV 327

[Close]

BLAST of CSPI02G00240 vs. TAIR 10
Match: AT1G23820.1 (spermidine synthase 1 )

HSP 1 Score: 135.6 bits (340), Expect = 7.4e-32
Identity = 82/259 (31.66%), Postives = 132/259 (50.97%), Query Frame = 0


Query: 28  LPQWFEE--ELEDDLKWSFAVNRVLHATTSEFQDIVLLDTKRFGKALLLDGKLQSAEKDE 87
           +P WF E   +      S  V +VL    S++QD+++  +  +GK L+LDG +Q  E+DE
Sbjct: 88  IPGWFSEMSPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDE 147

Query: 88  FIYHESLVHPALLLHHNPKTVFIMGGGEGCTARETLKHKSIEKVVMCDIDRDVVNFCRAH 147
             Y E + H  L    NPK V ++GGG+G   RE  +H SIE++ MC+ID+ VV+  +  
Sbjct: 148 CAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQF 207

Query: 148 LKENQDAFQDERLHIIFDDAKAGLEGRPE-KFDVIIGDLSDPHEGGPCNHLYTKSFYEDV 207
             +    ++D R++++  D  A L+   E  +D +I D SDP   GP   L+ K F++ V
Sbjct: 208 FPDVAIGYEDPRVNLVIGDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQSV 267

Query: 208 IKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHVFRYVIAYT-AHVPSYADS-CGWVL 267
            +  L   G+  TQA     L   +   I +  + +F+  + Y    VP+Y     G++L
Sbjct: 268 AR-ALRPGGVVCTQAESLW-LHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPSGVIGFML 327

Query: 268 ASDHPIKLDIEDLNNKIRE 282
            S     +D +   N I E
Sbjct: 328 CSTEGPDVDFKHPLNPIDE 342

[Close]

BLAST of CSPI02G00240 vs. TAIR 10
Match: AT1G23820.2 (spermidine synthase 1 )

HSP 1 Score: 133.7 bits (335), Expect = 2.8e-31
Identity = 78/241 (32.37%), Postives = 125/241 (51.87%), Query Frame = 0


Query: 28  LPQWFEE--ELEDDLKWSFAVNRVLHATTSEFQDIVLLDTKRFGKALLLDGKLQSAEKDE 87
           +P WF E   +      S  V +VL    S++QD+++  +  +GK L+LDG +Q  E+DE
Sbjct: 88  IPGWFSEMSPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDE 147

Query: 88  FIYHESLVHPALLLHHNPKTVFIMGGGEGCTARETLKHKSIEKVVMCDIDRDVVNFCRAH 147
             Y E + H  L    NPK V ++GGG+G   RE  +H SIE++ MC+ID+ VV+  +  
Sbjct: 148 CAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQF 207

Query: 148 LKENQDAFQDERLHIIFDDAKAGLEGRPE-KFDVIIGDLSDPHEGGPCNHLYTKSFYEDV 207
             +    ++D R++++  D  A L+   E  +D +I D SDP   GP   L+ K F++ V
Sbjct: 208 FPDVAIGYEDPRVNLVIGDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQSV 267

Query: 208 IKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHVFRYVIAYT-AHVPSYADSCG-WVL 264
            +  L   G+  TQA     L   +   I +  + +F+  + Y    VP+Y    G W+ 
Sbjct: 268 AR-ALRPGGVVCTQAESLW-LHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPRHWGHWIY 324

[Close]

BLAST of CSPI02G00240 vs. TAIR 10
Match: AT1G70310.1 (spermidine synthase 2 )

HSP 1 Score: 133.7 bits (335), Expect = 2.8e-31
Identity = 78/251 (31.08%), Postives = 130/251 (51.79%), Query Frame = 0


Query: 28  LPQWFEE--ELEDDLKWSFAVNRVLHATTSEFQDIVLLDTKRFGKALLLDGKLQSAEKDE 87
           +P WF E   +      S  V ++L    S++QD+++  +  +GK L+LDG +Q  E+DE
Sbjct: 48  IPGWFSEISPMWPGEAHSLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDE 107

Query: 88  FIYHESLVHPALLLHHNPKTVFIMGGGEGCTARETLKHKSIEKVVMCDIDRDVVNFCRAH 147
             Y E + H  L    NPK V ++GGG+G   RE  +H S+E++ +C+ID+ VV+  + +
Sbjct: 108 CAYQEMITHLPLCSISNPKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQY 167

Query: 148 LKENQDAFQDERLHIIFDDAKAGLEGRPE-KFDVIIGDLSDPHEGGPCNHLYTKSFYEDV 207
                  ++D R+++I  D  A L+   E  +D +I D SDP   GP   L+ K F+E V
Sbjct: 168 FPNVAVGYEDPRVNLIIGDGVAFLKNAAEGTYDAVIVDSSDPI--GPAKELFEKPFFESV 227

Query: 208 IKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHVFRYVIAYT-AHVPSYADS-CGWVL 267
            +  L   G+  TQA     L   +   I +  + +F+  + Y    VP+Y     G++L
Sbjct: 228 NR-ALRPGGVVCTQAESLW-LHMDIIEDIVSNCRDIFKGSVNYAWTSVPTYPSGVIGFML 287

Query: 268 ASDHPIKLDIE 274
            S    ++D +
Sbjct: 288 CSSEGPQVDFK 294

The following BLAST results are available for this feature:

BLAST of CSPI02G00240 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S7X6	7.8e-119	64.92	Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV...	[more]
Q975S5	3.5e-58	41.94	Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / ...	[more]
Q72K55	1.4e-54	40.42	Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / ...	[more]
Q5SK28	1.4e-54	40.42	Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DS...	[more]
Q9YE02	2.3e-54	40.80	Polyamine aminopropyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 1...	[more]

BLAST of CSPI02G00240 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LET5	2.6e-194	100.00	PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000240 PE=3 S...	[more]
A0A5A7UZV1	3.1e-187	96.40	Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A1S3CAR3	3.1e-187	96.40	thermospermine synthase ACAULIS5-like OS=Cucumis melo OX=3656 GN=LOC103498720 PE...	[more]
A0A6J1G4J8	1.3e-164	84.08	thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111450...	[more]
A0A6J1KJ05	4.5e-162	82.88	thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC11149366...	[more]

BLAST of CSPI02G00240 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141174.1	5.5e-194	100.00	thermospermine synthase ACAULIS5 [Cucumis sativus] >KGN60515.1 hypothetical prot...	[more]
XP_008459675.1	6.5e-187	96.40	PREDICTED: thermospermine synthase ACAULIS5-like [Cucumis melo] >KAA0061473.1 th...	[more]
XP_038890496.1	1.8e-181	91.59	thermospermine synthase ACAULIS5-like [Benincasa hispida]	[more]
XP_022946737.1	2.6e-164	84.08	thermospermine synthase ACAULIS5-like [Cucurbita moschata]	[more]
KAG7030499.1	4.5e-164	83.48	Thermospermine synthase ACAULIS5 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of CSPI02G00240 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19530.1	5.6e-120	64.92	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G19530.2	5.6e-120	64.92	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G23820.1	7.4e-32	31.66	spermidine synthase 1	[more]
AT1G23820.2	2.8e-31	32.37	spermidine synthase 1	[more]
AT1G70310.1	2.8e-31	31.08	spermidine synthase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 82..332 e-value: 5.8E-73 score: 247.5
None	No IPR available	PFAM	PF01564	Spermine_synth	coord: 85..257 e-value: 3.6E-38 score: 130.9
None	No IPR available	PANTHER	PTHR43317:SF6	THERMOSPERMINE SYNTHASE ACAULIS5-LIKE	coord: 3..331
None	No IPR available	PANTHER	PTHR43317	THERMOSPERMINE SYNTHASE ACAULIS5	coord: 3..331
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 110..218 e-value: 1.09408E-6 score: 44.7283
IPR035246	Spermidine synthase, tetramerisation domain	PFAM	PF17284	Spermine_synt_N	coord: 30..82 e-value: 2.8E-13 score: 49.5
IPR037163	Spermidine synthase, tetramerisation domain superfamily	GENE3D	2.30.140.10	Spermidine synthase, tetramerisation domain	coord: 29..81 e-value: 7.6E-17 score: 63.2
IPR001045	Spermidine/spermine synthases	HAMAP	MF_00198	Spermidine_synth	coord: 30..306 score: 30.710922
IPR030374	Polyamine biosynthesis domain	PROSITE	PS51006	PABS_2	coord: 29..265 score: 91.829514
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 28..307

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G00240.1	CSPI02G00240.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0006596	polyamine biosynthetic process
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0010487	thermospermine synthase activity
molecular_function	GO:0003824	catalytic activity