CSPI01G34670 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G34670
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	E3 ubiquitin-protein ligase BOI-like
Location	Chr1: 29657566 .. 29659049 (-)
RNA-Seq Expression	CSPI01G34670
Synteny	CSPI01G34670

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCAAAACAAACCCAATGGATTTAAATTAAAAACCCCAATCTTCTCCTTCCTTGTCGCCACCTCTATTTCATGCTCTTTTATTTATATTTATATTTTTTTTTATATATACATATTTCTCTCTATTAAGCAAGAAATTGTTGAGAAGATATCTCTAAGTCACAAGAAAAGCAAAGAAGAAACGAAACCAACTCAACAAGAAAATTTTCATGGCAGTTGAAGCACGGCCTATGAATCTTCTACCTTCTCATCAGTTCATCATCCCCAACGACAGGTCCATAATCTCAATCCATATATTTATGTATCTATATCTTCCTTTTCATTTTCTTTTTCTTTTTCAATGCTTTCCTCTAACACACACACCTCTCTCTTATTTTCTCAGAGATTTCATCAAACAGAATCAAGAATTAGCAAACACCCACATGACACCTGTCACGGACACCACCACCCCAGGGATAATATGGCCTTACACGGCCGTCAAACCAGCGGTTACACCGGAGACGACCCTCAATAATTTCGCCACTGTGTACGATTGGGGAAAAGCAGAGAGTGGACTTACATCCAATAACTTCCCATCAACCGCTCCCACCCGAAAGCGAACGAGATCGCGATCCTTTTACGATGAACCCGGGGGGAGGTTGTTGGATGAAGAAATCATAAACTCCCACATTCAGCAACAACAGAGTGAAATGGATCGGTTCATTGCAATCCATGTAAGTCCCAGATTTTTTTTTTTAATTAACTTCTGAGAGTGGGTTTAAAATGAAACGATGTACACTAGTTGATGAAACTGACATCGGTTTTAAATTACAGAGAGAGAAGATGAGGATTGAAATGGAGATGCGGAAGAAGAGGGAATCGGGGATGTTGGTAAGGGCAATAGAGGAAAGAGTGGTAAAGAAGTTGAAGGAAAAAGAAGAAGAAATAGAAAGAATGGGGAAATTGAATTGGGTGTTGCAAGAGAGAGTGAAACGGCTGTGTGTGGAGAATCAAGTATGGAGGGATTTGGCAGAGAGCAATGAAGCGACGGTGAATTGTTTAAGGAATAATTTGGAGCAGGTGATATTAATGGCGGCAAATAAGAACGTGGGAGGTGTGGCGGGGGCGAAGGAGAAGGAGGAGAAGGCGGAGTCAAGCTGTGGGAGTACAAGTGAGTGTGGAAGGAAGGAGGAGGAGGAGGAGGAGGCGGAGAGTGGTGGTGGAGGAGGGCGGTGCCGGAAGTGTGGGGCGGGGGAATCGAGGGTGTTGGTGTTGCCATGCAGGCATCTTTGTTTGTGTACAATGTGTGGGTCCACAATTCACAGTTGCCCAGTATGTAATTCTGCCATTAATGCCAGCGTCCATGTTAATTTTTCTTGAATTCATACCCAACAACACAAACAAACAAACATAATTCTTTTCTTTTTTTGGTACATAAAAATTTATTCTCTCTCTCTCTTTTACTTCTCATTCTTCCCATTCTTTTCTTTCTTTTTATTTATTATTAC

mRNA sequence

CCAAAACAAACCCAATGGATTTAAATTAAAAACCCCAATCTTCTCCTTCCTTGTCGCCACCTCTATTTCATGCTCTTTTATTTATATTTATATTTTTTTTTATATATACATATTTCTCTCTATTAAGCAAGAAATTGTTGAGAAGATATCTCTAAGTCACAAGAAAAGCAAAGAAGAAACGAAACCAACTCAACAAGAAAATTTTCATGGCAGTTGAAGCACGGCCTATGAATCTTCTACCTTCTCATCAGTTCATCATCCCCAACGACAGAGATTTCATCAAACAGAATCAAGAATTAGCAAACACCCACATGACACCTGTCACGGACACCACCACCCCAGGGATAATATGGCCTTACACGGCCGTCAAACCAGCGGTTACACCGGAGACGACCCTCAATAATTTCGCCACTGTGTACGATTGGGGAAAAGCAGAGAGTGGACTTACATCCAATAACTTCCCATCAACCGCTCCCACCCGAAAGCGAACGAGATCGCGATCCTTTTACGATGAACCCGGGGGGAGGTTGTTGGATGAAGAAATCATAAACTCCCACATTCAGCAACAACAGAGTGAAATGGATCGGTTCATTGCAATCCATAGAGAGAAGATGAGGATTGAAATGGAGATGCGGAAGAAGAGGGAATCGGGGATGTTGGTAAGGGCAATAGAGGAAAGAGTGGTAAAGAAGTTGAAGGAAAAAGAAGAAGAAATAGAAAGAATGGGGAAATTGAATTGGGTGTTGCAAGAGAGAGTGAAACGGCTGTGTGTGGAGAATCAAGTATGGAGGGATTTGGCAGAGAGCAATGAAGCGACGGTGAATTGTTTAAGGAATAATTTGGAGCAGGTGATATTAATGGCGGCAAATAAGAACGTGGGAGGTGTGGCGGGGGCGAAGGAGAAGGAGGAGAAGGCGGAGTCAAGCTGTGGGAGTACAAGTGAGTGTGGAAGGAAGGAGGAGGAGGAGGAGGAGGCGGAGAGTGGTGGTGGAGGAGGGCGGTGCCGGAAGTGTGGGGCGGGGGAATCGAGGGTGTTGGTGTTGCCATGCAGGCATCTTTGTTTGTGTACAATGTGTGGGTCCACAATTCACAGTTGCCCAGTATGTAATTCTGCCATTAATGCCAGCGTCCATGTTAATTTTTCTTGAATTCATACCCAACAACACAAACAAACAAACATAATTCTTTTCTTTTTTTGGTACATAAAAATTTATTCTCTCTCTCTCTTTTACTTCTCATTCTTCCCATTCTTTTCTTTCTTTTTATTTATTATTAC

Coding sequence (CDS)

ATGGCAGTTGAAGCACGGCCTATGAATCTTCTACCTTCTCATCAGTTCATCATCCCCAACGACAGAGATTTCATCAAACAGAATCAAGAATTAGCAAACACCCACATGACACCTGTCACGGACACCACCACCCCAGGGATAATATGGCCTTACACGGCCGTCAAACCAGCGGTTACACCGGAGACGACCCTCAATAATTTCGCCACTGTGTACGATTGGGGAAAAGCAGAGAGTGGACTTACATCCAATAACTTCCCATCAACCGCTCCCACCCGAAAGCGAACGAGATCGCGATCCTTTTACGATGAACCCGGGGGGAGGTTGTTGGATGAAGAAATCATAAACTCCCACATTCAGCAACAACAGAGTGAAATGGATCGGTTCATTGCAATCCATAGAGAGAAGATGAGGATTGAAATGGAGATGCGGAAGAAGAGGGAATCGGGGATGTTGGTAAGGGCAATAGAGGAAAGAGTGGTAAAGAAGTTGAAGGAAAAAGAAGAAGAAATAGAAAGAATGGGGAAATTGAATTGGGTGTTGCAAGAGAGAGTGAAACGGCTGTGTGTGGAGAATCAAGTATGGAGGGATTTGGCAGAGAGCAATGAAGCGACGGTGAATTGTTTAAGGAATAATTTGGAGCAGGTGATATTAATGGCGGCAAATAAGAACGTGGGAGGTGTGGCGGGGGCGAAGGAGAAGGAGGAGAAGGCGGAGTCAAGCTGTGGGAGTACAAGTGAGTGTGGAAGGAAGGAGGAGGAGGAGGAGGAGGCGGAGAGTGGTGGTGGAGGAGGGCGGTGCCGGAAGTGTGGGGCGGGGGAATCGAGGGTGTTGGTGTTGCCATGCAGGCATCTTTGTTTGTGTACAATGTGTGGGTCCACAATTCACAGTTGCCCAGTATGTAATTCTGCCATTAATGCCAGCGTCCATGTTAATTTTTCTTGA

Protein sequence

MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTPETTLNNFATVYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEPGGRLLDEEIINSHIQQQQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKLNWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEKEEKAESSCGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGSTIHSCPVCNSAINASVHVNFS*

Homology

[Close]

BLAST of CSPI01G34670 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.4e-48
Identity = 146/328 (44.51%), Postives = 191/328 (58.23%), Query Frame = 0


Query: 1   MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP 60
           MAV+A  MN+    QFI PN RD +K  QE  N      T    P I     AV+P    
Sbjct: 1   MAVQAHHMNIF--SQFISPN-RDCVK-FQENMNHGEFEFTGGEVPLITGESFAVEP---- 60

Query: 61  ETTLNNFATVYDWGKAESGLTSNNFPSTAPTRKRTRSRSF-------------YDEPGGR 120
                  A   ++ KAESGL S NF     + KR R   F             +D     
Sbjct: 61  ------LAAKANFNKAESGL-SYNFTVPPLSTKRQRDFQFSDSNAPVKRRSVAFDSSSPS 120

Query: 121 LLDEEIINSHIQQQQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKE 180
           L++ E+++    QQQSE+DRF+A   EK+RIE+E R++ ++ ML  A++  + KKLKEK+
Sbjct: 121 LINVELVSQIQNQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKLKEKD 180

Query: 181 EEIERMGKLNWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGV 240
           +EI R+  LNWVLQERVK L VENQ+WRD+A++NEA  N LR NL+QV+   A       
Sbjct: 181 DEIVRIRNLNWVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVL---AQLETFPT 240

Query: 241 AGAKEKEEKAESSCGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLC 300
           A A   E+ AESSCGS   CG   +   EA +  GGG C++CG  E+ VLVLPCRHLCLC
Sbjct: 241 ASA-VVEDDAESSCGSC--CG---DGGGEAVTAVGGG-CKRCGEREASVLVLPCRHLCLC 300

Query: 301 TMCGST--IHSCPVCNSAINASVHVNFS 314
           T+CG +  + +CPVC+  +NASVHVN S
Sbjct: 301 TVCGGSALLRTCPVCDMVMNASVHVNMS 303

[Close]

BLAST of CSPI01G34670 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 8.3e-46
Identity = 138/322 (42.86%), Postives = 189/322 (58.70%), Query Frame = 0


Query: 1   MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP 60
           MAVEAR MNL  S Q+I   +R+ +K     + T+M         G         P    
Sbjct: 1   MAVEARHMNLF-SSQYI--TNRECVK-----SQTNMNNGQQIAGGGF--------PVTIG 60

Query: 61  ETTLNNFATVYDWGKAESGLTS-------NNFPSTAPTRKRTRSRSFYDEPGGRLLDEEI 120
           +  L     +  + K+ES LT+       + F S A    + R R+    P   L+D E+
Sbjct: 61  DRNLQYIDPINSFNKSESELTAISKRQRDSTFDSDALIASQKR-RAIAFSPAS-LIDAEL 120

Query: 121 INSHIQQQQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERM 180
           + S IQQQ SE+DRF+A   E +RIE+E R++ ++ ML  A++  ++KKLK K+EEI RM
Sbjct: 121 V-SQIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRM 180

Query: 181 GKLNWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEK 240
           GKLNWVLQERVK L VENQ+WRDLA++NEAT N LR+NLEQV+    + +       +E 
Sbjct: 181 GKLNWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEA 240

Query: 241 EEKAESSCGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGST 300
           ++ AESSCGS       +  +  A   GG   C++CG   + VLVLPCRHLCLCT+CGS+
Sbjct: 241 DD-AESSCGSC------DGGDVTAVVNGG---CKRCGELTASVLVLPCRHLCLCTVCGSS 293

Query: 301 --IHSCPVCNSAINASVHVNFS 314
             + +CPVC+  + ASVHVN S
Sbjct: 301 ALLRTCPVCDMVMTASVHVNMS 293

[Close]

BLAST of CSPI01G34670 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 2.3e-43
Identity = 106/215 (49.30%), Postives = 147/215 (68.37%), Query Frame = 0


Query: 114 INSHIQQQQSEMDRFIAIH---REKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEI 173
           I+SH+ QQQ E+DRF+++H    E+++ E+E ++KR++  ++ AIE+ +VK+L+ KEEE 
Sbjct: 145 ISSHMNQQQHEIDRFVSLHLYQMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEER 204

Query: 174 ERMGKLNWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVG-GVAG 233
           ER+GK+N  L+ERVK L +ENQ+WRDLA++NEAT N LR NLE V+    + + G G+  
Sbjct: 205 ERIGKVNHALEERVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEK 264

Query: 234 AKEKEEKAESSCGSTSECGRKEEE--------EEEAESGGGGGR---CRKCGAGESRVLV 293
              +E+ AES CGS+  CG   EE        E EA+      R   CR CG  ES VL+
Sbjct: 265 NMNEEDDAESCCGSS--CGGGGEETVRRRVGLEREAQDKAERRRRRMCRNCGEEESCVLL 324

Query: 294 LPCRHLCLCTMCGSTIHSCPVCNSAINASVHVNFS 314
           LPCRHLCLC +CGS++H+CP+C S  NASVHVN S
Sbjct: 325 LPCRHLCLCGVCGSSVHTCPICTSPKNASVHVNMS 357

[Close]

BLAST of CSPI01G34670 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 4.4e-39
Identity = 104/261 (39.85%), Postives = 158/261 (60.54%), Query Frame = 0


Query: 77  ESGLTSN-----NFPSTAPTRKRTRSRSFYDEPGGRLLDE-----------EIINSHIQQ 136
           +S LT N     +F     +RKR+R  S    P   +  +           + ++S++QQ
Sbjct: 77  DSSLTFNSDNNVDFLRPVSSRKRSREESVVLNPSAYMQIQKNPTDPLMFLGQDLSSNVQQ 136

Query: 137 QQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKLNWVL 196
              ++DR I+ H E+MR+E+E ++K +   +V A+E+ ++K L+ K++EI  +GKLN  L
Sbjct: 137 HHFDIDRLISNHVERMRMEIEEKRKTQGRRIVEAVEQGLMKTLRAKDDEINHIGKLNLFL 196

Query: 197 QERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEKEEKAESS 256
           +E+VK LCVENQ+WRD+A+SNEATVN LR+NL+QV L A  +N           + A+S 
Sbjct: 197 EEKVKSLCVENQIWRDVAQSNEATVNALRSNLQQV-LAAVERN--RWEEPPTVADDAQSC 256

Query: 257 CGSTSECGRKEE--------EEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGS 314
           CGS  E   +EE        ++ +     G   CR CG GE+ VL+LPCRH+CLC++CGS
Sbjct: 257 CGSNDEGDSEEERWKLAGEAQDTKKMCRVGMSMCRSCGKGEASVLLLPCRHMCLCSVCGS 316

[Close]

BLAST of CSPI01G34670 vs. ExPASy TrEMBL
Match: A0A0A0M3V5 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G707120 PE=4 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.0e-171
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 0


Query: 1   MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP 60
           MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP
Sbjct: 1   MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP 60

Query: 61  ETTLNNFATVYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEPGGRLLDEEIINSHIQQ 120
           ETTLNNFATVYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEPGGRLLDEEIINSHIQQ
Sbjct: 61  ETTLNNFATVYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEPGGRLLDEEIINSHIQQ 120

Query: 121 QQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKLNWVL 180
           QQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKLNWVL
Sbjct: 121 QQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKLNWVL 180

Query: 181 QERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEKEEKAESS 240
           QERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEKEEKAESS
Sbjct: 181 QERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEKEEKAESS 240

Query: 241 CGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGSTIHSCPVC 300
           CGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGSTIHSCPVC
Sbjct: 241 CGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGSTIHSCPVC 300

Query: 301 NSAINASVHVNFS 314
           NSAINASVHVNFS
Sbjct: 301 NSAINASVHVNFS 313

[Close]

BLAST of CSPI01G34670 vs. ExPASy TrEMBL
Match: A0A1S3C8U5 (E3 ubiquitin-protein ligase BOI-like OS=Cucumis melo OX=3656 GN=LOC103498284 PE=4 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.6e-164
Identity = 305/314 (97.13%), Postives = 310/314 (98.73%), Query Frame = 0


Query: 1   MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP 60
           MAVEARPMNLLPSHQFIIPN+RDFIKQNQELA+THMTP TDTTTPGIIWPYTAVKPAVTP
Sbjct: 1   MAVEARPMNLLPSHQFIIPNNRDFIKQNQELASTHMTPTTDTTTPGIIWPYTAVKPAVTP 60

Query: 61  ETTLNNFA-TVYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEPGGRLLDEEIINSHIQ 120
           E TLNNFA T+YDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEPGGRLLDEEIINSHIQ
Sbjct: 61  EATLNNFATTMYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEPGGRLLDEEIINSHIQ 120

Query: 121 QQQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKLNWV 180
           QQQSEMDRFIAIHREKMRIEME+RKKRESGMLVRAIEERVVKKLKEKEEEIERMGKLNWV
Sbjct: 121 QQQSEMDRFIAIHREKMRIEMEIRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKLNWV 180

Query: 181 LQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEKEEKAES 240
           LQERVKRLC+ENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEKEEKAES
Sbjct: 181 LQERVKRLCMENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEKEEKAES 240

Query: 241 SCGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGSTIHSCPV 300
           SCGSTSECGRKEEEEEEAES GGGGRCRKCGAGESRVLVLPCRHLCLCTMCGSTIHSCPV
Sbjct: 241 SCGSTSECGRKEEEEEEAES-GGGGRCRKCGAGESRVLVLPCRHLCLCTMCGSTIHSCPV 300

Query: 301 CNSAINASVHVNFS 314
           CNSAINASVHVNFS
Sbjct: 301 CNSAINASVHVNFS 313

[Close]

BLAST of CSPI01G34670 vs. ExPASy TrEMBL
Match: A0A6J1EP88 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111434333 PE=4 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 1.1e-90
Identity = 210/317 (66.25%), Postives = 236/317 (74.45%), Query Frame = 0


Query: 1   MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP 60
           MAVEAR MN LPS    I N+ DF+KQ+QE+ + +        T G +WPY A K    P
Sbjct: 1   MAVEARRMNHLPSP--FITNNGDFMKQSQEVLDIYNYKTMPVETAG-LWPYVAGKS--VP 60

Query: 61  ETTLNNFATVYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEP----GGRLLDEEIINS 120
           E  +NNF        A+SGLTSNNFP+ AP RKR R RS Y+EP      RL DEEIINS
Sbjct: 61  E-IVNNF-------PADSGLTSNNFPA-APVRKRQR-RSGYEEPPCPFPCRLSDEEIINS 120

Query: 121 HIQQQQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKL 180
           H+QQQQSE+DRFIAIHREK+R+ ME+RKKRESGMLVRAIEER+VKKLKEK+EEIERMGKL
Sbjct: 121 HVQQQQSEIDRFIAIHREKVRMAMEIRKKRESGMLVRAIEERLVKKLKEKDEEIERMGKL 180

Query: 181 NWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEKEEK 240
           NWVLQERVKRLC+ENQ+WRDLAE+NEATVN LR NLE++I  AAN               
Sbjct: 181 NWVLQERVKRLCMENQLWRDLAETNEATVNSLRTNLERLI-SAANDTAAAA-------ND 240

Query: 241 AESSCGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGSTIHS 300
           AESSCGS S CG  EEE       GGG RCRKC AGESRVLVLPCRHLCLCTMCGST+H+
Sbjct: 241 AESSCGSNSGCGITEEE------SGGGRRCRKCMAGESRVLVLPCRHLCLCTMCGSTLHT 288

Query: 301 CPVCNSAINASVHVNFS 314
           CPVC+SAINASVHVNFS
Sbjct: 301 CPVCDSAINASVHVNFS 288

[Close]

BLAST of CSPI01G34670 vs. ExPASy TrEMBL
Match: A0A6J1KKD3 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111495377 PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 7.5e-90
Identity = 211/317 (66.56%), Postives = 237/317 (74.76%), Query Frame = 0


Query: 1   MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP 60
           MAVEAR MN LPS    I N+ DF+KQ+QE+ +          T G +WPY + K    P
Sbjct: 1   MAVEARRMNHLPSP--FITNNGDFMKQSQEVLDICNYKTMPAETAG-LWPYISGKS--VP 60

Query: 61  ETTLNNFATVYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEP----GGRLLDEEIINS 120
           E  +NNF        A+SGLTSNNFP+ AP RKR R RS Y+EP      RL DEEIINS
Sbjct: 61  E-IVNNF-------PADSGLTSNNFPA-APVRKRQR-RSGYEEPPCPFPCRLSDEEIINS 120

Query: 121 HIQQQQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKL 180
           H+QQQQSE+DRFIAIHREK+R+ ME+RKKRESGMLVRAIEER+VKKLKEK+EEIERMGKL
Sbjct: 121 HVQQQQSEIDRFIAIHREKVRMAMEVRKKRESGMLVRAIEERLVKKLKEKDEEIERMGKL 180

Query: 181 NWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEKEEK 240
           NWVLQERVKRLC+ENQ+WRDLAE+NEATVN LR NLE++I  A   N    AG       
Sbjct: 181 NWVLQERVKRLCMENQLWRDLAETNEATVNSLRTNLERLISAA---NETAAAG-----ND 240

Query: 241 AESSCGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGSTIHS 300
           AESSCGS    G  EEEEE  E  GGG RCRKC AGESRVLVLPCRHLCLCTMCGST+H+
Sbjct: 241 AESSCGSNRGNGMTEEEEE--EESGGGRRCRKCMAGESRVLVLPCRHLCLCTMCGSTLHT 292

Query: 301 CPVCNSAINASVHVNFS 314
           CPVC+SAINASVHVNFS
Sbjct: 301 CPVCDSAINASVHVNFS 292

[Close]

BLAST of CSPI01G34670 vs. ExPASy TrEMBL
Match: A0A6J1GUS0 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111457113 PE=4 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 5.7e-74
Identity = 178/313 (56.87%), Postives = 206/313 (65.81%), Query Frame = 0


Query: 1   MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP 60
           MAVEARP+NL PS    I N RDFI  N   A     P+   T P               
Sbjct: 1   MAVEARPINLFPSTPHFITN-RDFIDPNTPTA----PPLHSLTFP--------------- 60

Query: 61  ETTLNNFATVYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEPGGRLLDEEIINSHIQQ 120
                       +G  ++GLTS       P+RKR R  S YDE   RL D+E+I SHI+ 
Sbjct: 61  -----------MYGNPDTGLTS------TPSRKRARC-SVYDESPPRLFDQEMIISHIEH 120

Query: 121 QQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKLNWVL 180
           Q+SE+DRFIAIH EK+ +EME RK+R+S MLV AIE+RVVKKLKEKEEEIERMGKLNWVL
Sbjct: 121 QRSEIDRFIAIHTEKVMMEMEERKQRQSRMLVSAIEDRVVKKLKEKEEEIERMGKLNWVL 180

Query: 181 QERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEKEEKAESS 240
           QERVK L +ENQ WR+LAE+NEAT+N LR NLEQV++  A +    VA      + AESS
Sbjct: 181 QERVKHLSLENQQWRNLAETNEATLNSLRTNLEQVLMANAGEEAAAVA------DDAESS 240

Query: 241 CGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGSTIHSCPVC 300
           CGS             + SGGGGGRCRKCGA ESRVLVLPCRHLCLCT+CGS +H+CPVC
Sbjct: 241 CGS-------------SYSGGGGGRCRKCGAAESRVLVLPCRHLCLCTICGSRLHTCPVC 256

Query: 301 NSAINASVHVNFS 314
           NSAINASVHVNFS
Sbjct: 301 NSAINASVHVNFS 256

[Close]

BLAST of CSPI01G34670 vs. NCBI nr
Match: XP_004145480.2 (BOI-related E3 ubiquitin-protein ligase 1 [Cucumis sativus] >KGN66896.1 hypothetical protein Csa_007333 [Cucumis sativus])

HSP 1 Score: 610.9 bits (1574), Expect = 6.1e-171
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 0


Query: 1   MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP 60
           MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP
Sbjct: 1   MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP 60

Query: 61  ETTLNNFATVYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEPGGRLLDEEIINSHIQQ 120
           ETTLNNFATVYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEPGGRLLDEEIINSHIQQ
Sbjct: 61  ETTLNNFATVYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEPGGRLLDEEIINSHIQQ 120

Query: 121 QQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKLNWVL 180
           QQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKLNWVL
Sbjct: 121 QQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKLNWVL 180

Query: 181 QERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEKEEKAESS 240
           QERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEKEEKAESS
Sbjct: 181 QERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEKEEKAESS 240

Query: 241 CGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGSTIHSCPVC 300
           CGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGSTIHSCPVC
Sbjct: 241 CGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGSTIHSCPVC 300

Query: 301 NSAINASVHVNFS 314
           NSAINASVHVNFS
Sbjct: 301 NSAINASVHVNFS 313

[Close]

BLAST of CSPI01G34670 vs. NCBI nr
Match: XP_008459064.1 (PREDICTED: E3 ubiquitin-protein ligase BOI-like [Cucumis melo])

HSP 1 Score: 588.6 bits (1516), Expect = 3.2e-164
Identity = 305/314 (97.13%), Postives = 310/314 (98.73%), Query Frame = 0


Query: 1   MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP 60
           MAVEARPMNLLPSHQFIIPN+RDFIKQNQELA+THMTP TDTTTPGIIWPYTAVKPAVTP
Sbjct: 1   MAVEARPMNLLPSHQFIIPNNRDFIKQNQELASTHMTPTTDTTTPGIIWPYTAVKPAVTP 60

Query: 61  ETTLNNFA-TVYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEPGGRLLDEEIINSHIQ 120
           E TLNNFA T+YDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEPGGRLLDEEIINSHIQ
Sbjct: 61  EATLNNFATTMYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEPGGRLLDEEIINSHIQ 120

Query: 121 QQQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKLNWV 180
           QQQSEMDRFIAIHREKMRIEME+RKKRESGMLVRAIEERVVKKLKEKEEEIERMGKLNWV
Sbjct: 121 QQQSEMDRFIAIHREKMRIEMEIRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKLNWV 180

Query: 181 LQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEKEEKAES 240
           LQERVKRLC+ENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEKEEKAES
Sbjct: 181 LQERVKRLCMENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEKEEKAES 240

Query: 241 SCGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGSTIHSCPV 300
           SCGSTSECGRKEEEEEEAES GGGGRCRKCGAGESRVLVLPCRHLCLCTMCGSTIHSCPV
Sbjct: 241 SCGSTSECGRKEEEEEEAES-GGGGRCRKCGAGESRVLVLPCRHLCLCTMCGSTIHSCPV 300

Query: 301 CNSAINASVHVNFS 314
           CNSAINASVHVNFS
Sbjct: 301 CNSAINASVHVNFS 313

[Close]

BLAST of CSPI01G34670 vs. NCBI nr
Match: XP_038893892.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Benincasa hispida])

HSP 1 Score: 439.9 bits (1130), Expect = 1.9e-119
Identity = 254/329 (77.20%), Postives = 276/329 (83.89%), Query Frame = 0


Query: 1   MAVEARPMNLLPSHQFIIPNDRDFIKQNQEL---------ANTHMTPVTDTTTPGIIWPY 60
           MAVEAR MNLLPS QFIIPN+RDFIKQNQ +          NT M P T+  TPG++ PY
Sbjct: 1   MAVEARRMNLLPS-QFIIPNNRDFIKQNQGIVDICNNHNKTNTQMAPATE--TPGLL-PY 60

Query: 61  TAVKPAVTPETTLNNFATVYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEPG----GR 120
            A+     P  T+NNFA +YDWGKAESGLTSNNFP+ AP+RKRTR RS YDEPG    GR
Sbjct: 61  AAL-----PPETVNNFA-MYDWGKAESGLTSNNFPTVAPSRKRTR-RSVYDEPGGSFPGR 120

Query: 121 LLDEEIINSHIQQQQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKE 180
           LLDEEIINSHIQQQQSE+DRFIAIHREK+R+EME+RKKRESGMLVRAIEERVVKKLKEKE
Sbjct: 121 LLDEEIINSHIQQQQSEIDRFIAIHREKVRMEMEVRKKRESGMLVRAIEERVVKKLKEKE 180

Query: 181 EEIERMGKLNWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGV 240
           EEIERMGKLNWVLQERVKRLC+ENQVWRDLAESNEATVN LR+NLEQV+L AAN NV  V
Sbjct: 181 EEIERMGKLNWVLQERVKRLCIENQVWRDLAESNEATVNSLRSNLEQVML-AANNNV--V 240

Query: 241 AGAKEKEEKAESSCGSTSECGRKEEEEEEAESGG---GGGRCRKCGAGESRVLVLPCRHL 300
           A A    E+AESSCGSTS+CGR+  EEEE   GG   GGGRCRKCGAGESRVLVLPCRHL
Sbjct: 241 AAA----EEAESSCGSTSDCGRRGAEEEEESGGGGGAGGGRCRKCGAGESRVLVLPCRHL 300

Query: 301 CLCTMCGSTIHSCPVCNSAINASVHVNFS 314
           CLCTMCGST+H+CPVCNSAINASVHVNFS
Sbjct: 301 CLCTMCGSTLHTCPVCNSAINASVHVNFS 311

[Close]

BLAST of CSPI01G34670 vs. NCBI nr
Match: XP_022927535.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita moschata] >KAG6583800.1 BOI-related E3 ubiquitin-protein ligase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019425.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 343.2 bits (879), Expect = 2.4e-90
Identity = 210/317 (66.25%), Postives = 236/317 (74.45%), Query Frame = 0


Query: 1   MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP 60
           MAVEAR MN LPS    I N+ DF+KQ+QE+ + +        T G +WPY A K    P
Sbjct: 1   MAVEARRMNHLPSP--FITNNGDFMKQSQEVLDIYNYKTMPVETAG-LWPYVAGKS--VP 60

Query: 61  ETTLNNFATVYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEP----GGRLLDEEIINS 120
           E  +NNF        A+SGLTSNNFP+ AP RKR R RS Y+EP      RL DEEIINS
Sbjct: 61  E-IVNNF-------PADSGLTSNNFPA-APVRKRQR-RSGYEEPPCPFPCRLSDEEIINS 120

Query: 121 HIQQQQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKL 180
           H+QQQQSE+DRFIAIHREK+R+ ME+RKKRESGMLVRAIEER+VKKLKEK+EEIERMGKL
Sbjct: 121 HVQQQQSEIDRFIAIHREKVRMAMEIRKKRESGMLVRAIEERLVKKLKEKDEEIERMGKL 180

Query: 181 NWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEKEEK 240
           NWVLQERVKRLC+ENQ+WRDLAE+NEATVN LR NLE++I  AAN               
Sbjct: 181 NWVLQERVKRLCMENQLWRDLAETNEATVNSLRTNLERLI-SAANDTAAAA-------ND 240

Query: 241 AESSCGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGSTIHS 300
           AESSCGS S CG  EEE       GGG RCRKC AGESRVLVLPCRHLCLCTMCGST+H+
Sbjct: 241 AESSCGSNSGCGITEEE------SGGGRRCRKCMAGESRVLVLPCRHLCLCTMCGSTLHT 288

Query: 301 CPVCNSAINASVHVNFS 314
           CPVC+SAINASVHVNFS
Sbjct: 301 CPVCDSAINASVHVNFS 288

[Close]

BLAST of CSPI01G34670 vs. NCBI nr
Match: XP_023001155.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima])

HSP 1 Score: 340.5 bits (872), Expect = 1.5e-89
Identity = 211/317 (66.56%), Postives = 237/317 (74.76%), Query Frame = 0


Query: 1   MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP 60
           MAVEAR MN LPS    I N+ DF+KQ+QE+ +          T G +WPY + K    P
Sbjct: 1   MAVEARRMNHLPSP--FITNNGDFMKQSQEVLDICNYKTMPAETAG-LWPYISGKS--VP 60

Query: 61  ETTLNNFATVYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEP----GGRLLDEEIINS 120
           E  +NNF        A+SGLTSNNFP+ AP RKR R RS Y+EP      RL DEEIINS
Sbjct: 61  E-IVNNF-------PADSGLTSNNFPA-APVRKRQR-RSGYEEPPCPFPCRLSDEEIINS 120

Query: 121 HIQQQQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKL 180
           H+QQQQSE+DRFIAIHREK+R+ ME+RKKRESGMLVRAIEER+VKKLKEK+EEIERMGKL
Sbjct: 121 HVQQQQSEIDRFIAIHREKVRMAMEVRKKRESGMLVRAIEERLVKKLKEKDEEIERMGKL 180

Query: 181 NWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEKEEK 240
           NWVLQERVKRLC+ENQ+WRDLAE+NEATVN LR NLE++I  A   N    AG       
Sbjct: 181 NWVLQERVKRLCMENQLWRDLAETNEATVNSLRTNLERLISAA---NETAAAG-----ND 240

Query: 241 AESSCGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGSTIHS 300
           AESSCGS    G  EEEEE  E  GGG RCRKC AGESRVLVLPCRHLCLCTMCGST+H+
Sbjct: 241 AESSCGSNRGNGMTEEEEE--EESGGGRRCRKCMAGESRVLVLPCRHLCLCTMCGSTLHT 292

Query: 301 CPVCNSAINASVHVNFS 314
           CPVC+SAINASVHVNFS
Sbjct: 301 CPVCDSAINASVHVNFS 292

[Close]

BLAST of CSPI01G34670 vs. TAIR 10
Match: AT4G19700.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 194.9 bits (494), Expect = 9.7e-50
Identity = 146/328 (44.51%), Postives = 191/328 (58.23%), Query Frame = 0


Query: 1   MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP 60
           MAV+A  MN+    QFI PN RD +K  QE  N      T    P I     AV+P    
Sbjct: 1   MAVQAHHMNIF--SQFISPN-RDCVK-FQENMNHGEFEFTGGEVPLITGESFAVEP---- 60

Query: 61  ETTLNNFATVYDWGKAESGLTSNNFPSTAPTRKRTRSRSF-------------YDEPGGR 120
                  A   ++ KAESGL S NF     + KR R   F             +D     
Sbjct: 61  ------LAAKANFNKAESGL-SYNFTVPPLSTKRQRDFQFSDSNAPVKRRSVAFDSSSPS 120

Query: 121 LLDEEIINSHIQQQQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKE 180
           L++ E+++    QQQSE+DRF+A   EK+RIE+E R++ ++ ML  A++  + KKLKEK+
Sbjct: 121 LINVELVSQIQNQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKLKEKD 180

Query: 181 EEIERMGKLNWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGV 240
           +EI R+  LNWVLQERVK L VENQ+WRD+A++NEA  N LR NL+QV+   A       
Sbjct: 181 DEIVRIRNLNWVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVL---AQLETFPT 240

Query: 241 AGAKEKEEKAESSCGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLC 300
           A A   E+ AESSCGS   CG   +   EA +  GGG C++CG  E+ VLVLPCRHLCLC
Sbjct: 241 ASA-VVEDDAESSCGSC--CG---DGGGEAVTAVGGG-CKRCGEREASVLVLPCRHLCLC 300

Query: 301 TMCGST--IHSCPVCNSAINASVHVNFS 314
           T+CG +  + +CPVC+  +NASVHVN S
Sbjct: 301 TVCGGSALLRTCPVCDMVMNASVHVNMS 303

[Close]

BLAST of CSPI01G34670 vs. TAIR 10
Match: AT5G45100.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 185.7 bits (470), Expect = 5.9e-47
Identity = 138/322 (42.86%), Postives = 189/322 (58.70%), Query Frame = 0


Query: 1   MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP 60
           MAVEAR MNL  S Q+I   +R+ +K     + T+M         G         P    
Sbjct: 1   MAVEARHMNLF-SSQYI--TNRECVK-----SQTNMNNGQQIAGGGF--------PVTIG 60

Query: 61  ETTLNNFATVYDWGKAESGLTS-------NNFPSTAPTRKRTRSRSFYDEPGGRLLDEEI 120
           +  L     +  + K+ES LT+       + F S A    + R R+    P   L+D E+
Sbjct: 61  DRNLQYIDPINSFNKSESELTAISKRQRDSTFDSDALIASQKR-RAIAFSPAS-LIDAEL 120

Query: 121 INSHIQQQQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERM 180
           + S IQQQ SE+DRF+A   E +RIE+E R++ ++ ML  A++  ++KKLK K+EEI RM
Sbjct: 121 V-SQIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRM 180

Query: 181 GKLNWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVAGAKEK 240
           GKLNWVLQERVK L VENQ+WRDLA++NEAT N LR+NLEQV+    + +       +E 
Sbjct: 181 GKLNWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEA 240

Query: 241 EEKAESSCGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLCTMCGST 300
           ++ AESSCGS       +  +  A   GG   C++CG   + VLVLPCRHLCLCT+CGS+
Sbjct: 241 DD-AESSCGSC------DGGDVTAVVNGG---CKRCGELTASVLVLPCRHLCLCTVCGSS 293

Query: 301 --IHSCPVCNSAINASVHVNFS 314
             + +CPVC+  + ASVHVN S
Sbjct: 301 ALLRTCPVCDMVMTASVHVNMS 293

[Close]

BLAST of CSPI01G34670 vs. TAIR 10
Match: AT1G79110.2 (zinc ion binding )

HSP 1 Score: 183.0 bits (463), Expect = 3.8e-46
Identity = 106/212 (50.00%), Postives = 147/212 (69.34%), Query Frame = 0


Query: 114 INSHIQQQQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERM 173
           I+SH+ QQQ E+DRF+++H E+++ E+E ++KR++  ++ AIE+ +VK+L+ KEEE ER+
Sbjct: 145 ISSHMNQQQHEIDRFVSLHMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERI 204

Query: 174 GKLNWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVG-GVAGAKE 233
           GK+N  L+ERVK L +ENQ+WRDLA++NEAT N LR NLE V+    + + G G+     
Sbjct: 205 GKVNHALEERVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMN 264

Query: 234 KEEKAESSCGSTSECGRKEEE--------EEEAESGGGGGR---CRKCGAGESRVLVLPC 293
           +E+ AES CGS+  CG   EE        E EA+      R   CR CG  ES VL+LPC
Sbjct: 265 EEDDAESCCGSS--CGGGGEETVRRRVGLEREAQDKAERRRRRMCRNCGEEESCVLLLPC 324

Query: 294 RHLCLCTMCGSTIHSCPVCNSAINASVHVNFS 314
           RHLCLC +CGS++H+CP+C S  NASVHVN S
Sbjct: 325 RHLCLCGVCGSSVHTCPICTSPKNASVHVNMS 354

[Close]

BLAST of CSPI01G34670 vs. TAIR 10
Match: AT5G45100.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 180.3 bits (456), Expect = 2.5e-45
Identity = 121/267 (45.32%), Postives = 166/267 (62.17%), Query Frame = 0


Query: 56  PAVTPETTLNNFATVYDWGKAESGLTS-------NNFPSTAPTRKRTRSRSFYDEPGGRL 115
           P    +  L     +  + K+ES LT+       + F S A    + R R+    P   L
Sbjct: 13  PVTIGDRNLQYIDPINSFNKSESELTAISKRQRDSTFDSDALIASQKR-RAIAFSPAS-L 72

Query: 116 LDEEIINSHIQQQQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEE 175
           +D E++ S IQQQ SE+DRF+A   E +RIE+E R++ ++ ML  A++  ++KKLK K+E
Sbjct: 73  IDAELV-SQIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDE 132

Query: 176 EIERMGKLNWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVGGVA 235
           EI RMGKLNWVLQERVK L VENQ+WRDLA++NEAT N LR+NLEQV+    + +     
Sbjct: 133 EIIRMGKLNWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRP 192

Query: 236 GAKEKEEKAESSCGSTSECGRKEEEEEEAESGGGGGRCRKCGAGESRVLVLPCRHLCLCT 295
             +E ++ AESSCGS       +  +  A   GG   C++CG   + VLVLPCRHLCLCT
Sbjct: 193 LVEEADD-AESSCGSC------DGGDVTAVVNGG---CKRCGELTASVLVLPCRHLCLCT 252

Query: 296 MCGST--IHSCPVCNSAINASVHVNFS 314
           +CGS+  + +CPVC+  + ASVHVN S
Sbjct: 253 VCGSSALLRTCPVCDMVMTASVHVNMS 266

[Close]

BLAST of CSPI01G34670 vs. TAIR 10
Match: AT1G79110.1 (zinc ion binding )

HSP 1 Score: 177.6 bits (449), Expect = 1.6e-44
Identity = 106/215 (49.30%), Postives = 147/215 (68.37%), Query Frame = 0


Query: 114 INSHIQQQQSEMDRFIAIH---REKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEI 173
           I+SH+ QQQ E+DRF+++H    E+++ E+E ++KR++  ++ AIE+ +VK+L+ KEEE 
Sbjct: 145 ISSHMNQQQHEIDRFVSLHLYQMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEER 204

Query: 174 ERMGKLNWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAANKNVG-GVAG 233
           ER+GK+N  L+ERVK L +ENQ+WRDLA++NEAT N LR NLE V+    + + G G+  
Sbjct: 205 ERIGKVNHALEERVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEK 264

Query: 234 AKEKEEKAESSCGSTSECGRKEEE--------EEEAESGGGGGR---CRKCGAGESRVLV 293
              +E+ AES CGS+  CG   EE        E EA+      R   CR CG  ES VL+
Sbjct: 265 NMNEEDDAESCCGSS--CGGGGEETVRRRVGLEREAQDKAERRRRRMCRNCGEEESCVLL 324

Query: 294 LPCRHLCLCTMCGSTIHSCPVCNSAINASVHVNFS 314
           LPCRHLCLC +CGS++H+CP+C S  NASVHVN S
Sbjct: 325 LPCRHLCLCGVCGSSVHTCPICTSPKNASVHVNMS 357

The following BLAST results are available for this feature:

BLAST of CSPI01G34670 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81851	1.4e-48	44.51	E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1	[more]
Q9FHE4	8.3e-46	42.86	BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG...	[more]
F4IDI6	2.3e-43	49.30	Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37...	[more]
Q9LDD1	4.4e-39	39.85	Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37...	[more]

BLAST of CSPI01G34670 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M3V5	3.0e-171	100.00	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G707120 P...	[more]
A0A1S3C8U5	1.6e-164	97.13	E3 ubiquitin-protein ligase BOI-like OS=Cucumis melo OX=3656 GN=LOC103498284 PE=...	[more]
A0A6J1EP88	1.1e-90	66.25	BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1KKD3	7.5e-90	66.56	BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1GUS0	5.7e-74	56.87	BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita moschata OX=3662 GN=...	[more]

BLAST of CSPI01G34670 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145480.2	6.1e-171	100.00	BOI-related E3 ubiquitin-protein ligase 1 [Cucumis sativus] >KGN66896.1 hypothet...	[more]
XP_008459064.1	3.2e-164	97.13	PREDICTED: E3 ubiquitin-protein ligase BOI-like [Cucumis melo]	[more]
XP_038893892.1	1.9e-119	77.20	BOI-related E3 ubiquitin-protein ligase 1-like [Benincasa hispida]	[more]
XP_022927535.1	2.4e-90	66.25	BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita moschata] >KAG6583800....	[more]
XP_023001155.1	1.5e-89	66.56	BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima]	[more]

BLAST of CSPI01G34670 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19700.1	9.7e-50	44.51	SBP (S-ribonuclease binding protein) family protein	[more]
AT5G45100.1	5.9e-47	42.86	SBP (S-ribonuclease binding protein) family protein	[more]
AT1G79110.2	3.8e-46	50.00	zinc ion binding	[more]
AT5G45100.2	2.5e-45	45.32	SBP (S-ribonuclease binding protein) family protein	[more]
AT1G79110.1	1.6e-44	49.30	zinc ion binding	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 152..173
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 265..305 e-value: 3.1E-8 score: 33.3
None	No IPR available	PANTHER	PTHR42647:SF55	BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 1	coord: 1..312
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 232..312 e-value: 3.3E-11 score: 44.4
IPR017066	E3 ubiquitin-protein ligase BOI-like	PANTHER	PTHR42647	SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEIN	coord: 1..312
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 266..301 score: 8.874793

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G34670.1	CSPI01G34670.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0043067	regulation of programmed cell death
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity