CSPI01G34520 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G34520
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPhloem lectin
LocationChr1: 29442408 .. 29443674 (-)
RNA-Seq ExpressionCSPI01G34520
SyntenyCSPI01G34520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATATAGATATACATACATACATTATATATATATATATAGTTGATTTATCAAAGGTAATTAATTGAGGGTTGAAATGACTACGGAAGCGGAAGCAAAACAGCATTTGGTAAAAAAACTTGAGTATGACCATTGCTTAGAAACAATTTTAGCTCTCGCAGACGACCAAGCTGGAGTCGAGTGGCCAACCTTCCTTCCTCTTTGCAATCAACTATCTCAACGTGGCATCTCCCTCTGCAATGGAACTATCGTAAGTGTCTGCTTAAATTTAATATATTGATTTATTCTATAATCTAATATGCAAAAATAAATAAATATATATATATATATATATAATTGAGTTTTGATTATTAATTTTGTTGAATGCTTTCAGAAATTCAGAATTAATCTCGAAACAAACACTAATCGACTCTTCATATTACCAAAAATTCTCACTATTTTCGGGGTTAATGATCCCCGATACTGGAAATGGATCTACATCACGGACCATATGTATGTTTTTTTCCTTGTTAATTATTTCTAGAACTGAAATTCAATTTAATAATATTGTTATCTATCTATCTATATATATATTAATAAAAACAAAACGTTCAGTACTTCTGTGGATGCCCCTCAACTGATTGCAATACAAAAGCTCGACATACGTGGAGCATTTTCCGCACCATTGGTGACACCAAAAGTCTTAAACGTGCTATCATTATTGGTATTGCTCACTGAGAATACCGGCGGATGGCAGACTCCTTTGAACGTTACAATCACCACACCAGATGGGTCAACGAGACAGAGCCAAGTAAGCCTCGCGAGGAAGCCGATAGGCATTTTCTTTGAACTCACAGTAGGCGAGTTCACTCTCAATGACGATGGATGTAACAGTACCGGCCTAGTTAAGTTCAGCGTAACGGAATACTCAAACTATGAAAAACGTGGGATGCTGATCAAAGGTTGTATTGTTCGAGCAAAGTTTTCACTTGGCTGCACAAATCCAACTTTGGACCGTGCATAAAATTCTCTTTTCTCAACTCAAACTACATTTTGTATTTTGAGGCAATCTGGCTAAAGTTTACGTTTAATAATTACCCATATATAAAATGAAATACCAGACATGTAATAGCATAAAAAAGATTATATAAATAATACACAAACTGTTTTTGCATACTAGCAAAACGGTTATGTATAATGTTTTAAACACACTTTTTTCTCTTTTACATATCAATGTTTTAGAATATTATTTGAGAGAAAGAAATAAGAACTTGTTTGGAAGATTTAAC

mRNA sequence

AAATATAGATATACATACATACATTATATATATATATATAGTTGATTTATCAAAGGTAATTAATTGAGGGTTGAAATGACTACGGAAGCGGAAGCAAAACAGCATTTGGTAAAAAAACTTGAGTATGACCATTGCTTAGAAACAATTTTAGCTCTCGCAGACGACCAAGCTGGAGTCGAGTGGCCAACCTTCCTTCCTCTTTGCAATCAACTATCTCAACGTGGCATCTCCCTCTGCAATGGAACTATCAAATTCAGAATTAATCTCGAAACAAACACTAATCGACTCTTCATATTACCAAAAATTCTCACTATTTTCGGGGTTAATGATCCCCGATACTGGAAATGGATCTACATCACGGACCATATTACTTCTGTGGATGCCCCTCAACTGATTGCAATACAAAAGCTCGACATACGTGGAGCATTTTCCGCACCATTGGTGACACCAAAAGTCTTAAACGTGCTATCATTATTGGTATTGCTCACTGAGAATACCGGCGGATGGCAGACTCCTTTGAACGTTACAATCACCACACCAGATGGGTCAACGAGACAGAGCCAAGTAAGCCTCGCGAGGAAGCCGATAGGCATTTTCTTTGAACTCACAGTAGGCGAGTTCACTCTCAATGACGATGGATGTAACAGTACCGGCCTAGTTAAGTTCAGCGTAACGGAATACTCAAACTATGAAAAACGTGGGATGCTGATCAAAGGTTGTATTGTTCGAGCAAAGTTTTCACTTGGCTGCACAAATCCAACTTTGGACCGTGCATAAAATTCTCTTTTCTCAACTCAAACTACATTTTGTATTTTGAGGCAATCTGGCTAAAGTTTACGTTTAATAATTACCCATATATAAAATGAAATACCAGACATGTAATAGCATAAAAAAGATTATATAAATAATACACAAACTGTTTTTGCATACTAGCAAAACGGTTATGTATAATGTTTTAAACACACTTTTTTCTCTTTTACATATCAATGTTTTAGAATATTATTTGAGAGAAAGAAATAAGAACTTGTTTGGAAGATTTAAC

Coding sequence (CDS)

ATGACTACGGAAGCGGAAGCAAAACAGCATTTGGTAAAAAAACTTGAGTATGACCATTGCTTAGAAACAATTTTAGCTCTCGCAGACGACCAAGCTGGAGTCGAGTGGCCAACCTTCCTTCCTCTTTGCAATCAACTATCTCAACGTGGCATCTCCCTCTGCAATGGAACTATCAAATTCAGAATTAATCTCGAAACAAACACTAATCGACTCTTCATATTACCAAAAATTCTCACTATTTTCGGGGTTAATGATCCCCGATACTGGAAATGGATCTACATCACGGACCATATTACTTCTGTGGATGCCCCTCAACTGATTGCAATACAAAAGCTCGACATACGTGGAGCATTTTCCGCACCATTGGTGACACCAAAAGTCTTAAACGTGCTATCATTATTGGTATTGCTCACTGAGAATACCGGCGGATGGCAGACTCCTTTGAACGTTACAATCACCACACCAGATGGGTCAACGAGACAGAGCCAAGTAAGCCTCGCGAGGAAGCCGATAGGCATTTTCTTTGAACTCACAGTAGGCGAGTTCACTCTCAATGACGATGGATGTAACAGTACCGGCCTAGTTAAGTTCAGCGTAACGGAATACTCAAACTATGAAAAACGTGGGATGCTGATCAAAGGTTGTATTGTTCGAGCAAAGTTTTCACTTGGCTGCACAAATCCAACTTTGGACCGTGCATAA

Protein sequence

MTTEAEAKQHLVKKLEYDHCLETILALADDQAGVEWPTFLPLCNQLSQRGISLCNGTIKFRINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGAFSAPLVTPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKPIGIFFELTVGEFTLNDDGCNSTGLVKFSVTEYSNYEKRGMLIKGCIVRAKFSLGCTNPTLDRA*
Homology
BLAST of CSPI01G34520 vs. ExPASy Swiss-Prot
Match: C0HJV2 (Lectin OS=Luffa acutangula OX=56866 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 9.6e-23
Identity = 62/207 (29.95%), Postives = 115/207 (55.56%), Query Frame = 0

Query: 14  KLEYDHCLETILALADDQAGVEWPTFLPLCNQLSQRGISLCNGTIKFRINLETNTNRLFI 73
           +++  H LE IL   D       P+F+ L +Q++  GI L N T ++  +    +N   +
Sbjct: 7   EVKVGHNLEAILKGLDVDV-YSVPSFIKLYDQVT-AGIFLNNRTKRYWFDKNAESNCFML 66

Query: 74  LPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGAFSAPLVTPKVLNVLSL 133
             + L I    D RYW+W    +H  +++  +LI +  L+I G     +++P +    + 
Sbjct: 67  YARDLLITWSQDKRYWRWNPFQEHGNTLEVAELIDVCWLNIVGNIETSVLSPGISYEAAF 126

Query: 134 LVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKPIGIFFELTVGEFTLNDDGCNSTG 193
            V+LT +  GW+ P++V +  PDGS ++SQV+L  KP G++F ++VG F ++     + G
Sbjct: 127 EVMLTNSASGWRIPVDVKLKMPDGSEQESQVNLQDKPRGVWFFISVGHFKISVG--ETIG 186

Query: 194 LVKFSVTEYSNYEKRGMLIKGCIVRAK 221
            ++FS+ ++    KRG+L+KG +++ K
Sbjct: 187 NIEFSIVQHQE-AKRGLLVKGLVIQPK 208

BLAST of CSPI01G34520 vs. ExPASy Swiss-Prot
Match: O81865 (Protein PHLOEM PROTEIN 2-LIKE A1 OS=Arabidopsis thaliana OX=3702 GN=PP2A1 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 8.4e-11
Identity = 49/167 (29.34%), Postives = 85/167 (50.90%), Query Frame = 0

Query: 58  IKFRINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHIT-SVDAPQLIAIQKLDIRG 117
           IK+ ++ E N+N   +  K L+I   +D  YW W    +    +V+A  L  +  LDI G
Sbjct: 83  IKYWVD-ERNSNCFMLFAKNLSITWSDDVNYWTWFTEKESPNENVEAVGLKNVCWLDITG 142

Query: 118 AFSAPLVTPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTR--QSQVSLARKPIGIF 177
            F    +TP ++  +   V L +   GW TP+N+ +  P+G  +  + +VSL   P   +
Sbjct: 143 KFDTRNLTPGIVYEVVFKVKLEDPAYGWDTPVNLKLVLPNGKEKPQEKKVSLRELPRYKW 202

Query: 178 FELTVGEFTLNDDGCNSTGLVKFSVTEY-SNYEKRGMLIKGCIVRAK 221
            ++ VGEF       ++ G + FS+ E+ +   K+G+ +KG  +R K
Sbjct: 203 VDVRVGEFVPEK---SAAGEITFSMYEHAAGVWKKGLSLKGVAIRPK 245

BLAST of CSPI01G34520 vs. ExPASy Swiss-Prot
Match: Q9C8U9 (Uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 OS=Arabidopsis thaliana OX=3702 GN=PP2A4 PE=4 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 7.1e-10
Identity = 48/154 (31.17%), Postives = 77/154 (50.00%), Query Frame = 0

Query: 73  ILPKILTIFGVNDPRYWKWIYITDHITS---VDAPQLIAIQKLDIRGAFSAPLVTPKVLN 132
           I  + L+I   +   YW W+ +   I+S   VDA  L A+  LD+ G F    +T +   
Sbjct: 14  IYARDLSIAWSDKDEYWSWLPLRYDISSEKLVDAAVLEAVCWLDVNGKFDTRELTLETTY 73

Query: 133 VLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKP-IG-IFFELTVGEFTLNDD 192
            +  +V L +   GW  P+N+ +T PDG  R  + S+  K  IG  + +++ GEF  + D
Sbjct: 74  EVVYVVKLEDTASGWNIPVNLKLTLPDGKKRPQERSMCLKEHIGKRWIDISAGEFVTSPD 133

Query: 193 GCNSTGLVKFSVTE-YSNYEKRGMLIKGCIVRAK 221
              + G + FS+ E  S   KRG+ +K   +R K
Sbjct: 134 ---NAGEISFSMYETKSCCWKRGLFVKCVEIRPK 164

BLAST of CSPI01G34520 vs. ExPASy Swiss-Prot
Match: P0DSP5 (Lectin OS=Coccinia grandis OX=387127 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.6e-09
Identity = 40/149 (26.85%), Postives = 71/149 (47.65%), Query Frame = 0

Query: 62  INLETNTNRLFIL-PKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGAFSA 121
           +N E  ++  F+L P+  T+   +D RYW W  +      ++  QL  +   D R   + 
Sbjct: 1   LNQEKLSSTHFLLFPRAATLTWSDDTRYWSWNPVDFCGYQLEEAQLSRVSWFDCRWTVNT 60

Query: 122 PLVTPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKPIGIFFELTVG 181
             +   V   + L V +     GW TPLN+ +  P+GS + SQV L  +P  ++F+L +G
Sbjct: 61  TDLKTNVWYNVFLKVQMGSGASGWNTPLNLELEMPNGSKQASQVVLNDRPRDVWFKLQMG 120

Query: 182 EFTLNDDGCNSTGLVKFSVTEYSNYEKRG 210
              ++D    + G ++ S+  +    K G
Sbjct: 121 NLMVSDS--ETCGALRMSLYNHQTNWKMG 147

BLAST of CSPI01G34520 vs. ExPASy Swiss-Prot
Match: Q9ZVR1 (F-box protein PP2-B5 OS=Arabidopsis thaliana OX=3702 GN=PP2B5 PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 6.1e-09
Identity = 45/174 (25.86%), Postives = 83/174 (47.70%), Query Frame = 0

Query: 55  NGTIKFRINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDI 114
           NG + FR++  +    + +  K L I  V +P+YWKWI I +     + P+L+ I   DI
Sbjct: 112 NGKMSFRLDKASGKKCVMLSAKKLLISRVVNPKYWKWISIPESRFD-EVPELLNIDSFDI 171

Query: 115 RGAFSAPLVTPKVLNVLSLLVLLTENTGGWQT-PLNVTITTPDGSTRQSQVSL-ARKPIG 174
           RG  +  +++P       ++   T +  G+QT P+   +        ++ +   ++K   
Sbjct: 172 RGVLNTRIISPGTHYSAYIVYTKTSHFNGFQTSPIQAGVGFQRHGMSKTFIRFDSKKRQD 231

Query: 175 IFFELTVGEFTLNDDGCNSTGLVKFSVTEYSNYE----KRGMLIKGCIVRAKFS 223
            + E  +G+F  N+ G     L++ SV + + Y     K G++I+G   R K S
Sbjct: 232 GWMEAKIGDF-YNEGGLIGFNLIEVSVVDVARYPHMNMKSGLIIEGIEFRPKDS 283

BLAST of CSPI01G34520 vs. ExPASy TrEMBL
Match: A0A0A0M183 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G703060 PE=4 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 2.2e-131
Identity = 233/233 (100.00%), Postives = 233/233 (100.00%), Query Frame = 0

Query: 1   MTTEAEAKQHLVKKLEYDHCLETILALADDQAGVEWPTFLPLCNQLSQRGISLCNGTIKF 60
           MTTEAEAKQHLVKKLEYDHCLETILALADDQAGVEWPTFLPLCNQLSQRGISLCNGTIKF
Sbjct: 1   MTTEAEAKQHLVKKLEYDHCLETILALADDQAGVEWPTFLPLCNQLSQRGISLCNGTIKF 60

Query: 61  RINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGAFSA 120
           RINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGAFSA
Sbjct: 61  RINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGAFSA 120

Query: 121 PLVTPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKPIGIFFELTVG 180
           PLVTPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKPIGIFFELTVG
Sbjct: 121 PLVTPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKPIGIFFELTVG 180

Query: 181 EFTLNDDGCNSTGLVKFSVTEYSNYEKRGMLIKGCIVRAKFSLGCTNPTLDRA 234
           EFTLNDDGCNSTGLVKFSVTEYSNYEKRGMLIKGCIVRAKFSLGCTNPTLDRA
Sbjct: 181 EFTLNDDGCNSTGLVKFSVTEYSNYEKRGMLIKGCIVRAKFSLGCTNPTLDRA 233

BLAST of CSPI01G34520 vs. ExPASy TrEMBL
Match: A0A5D3CK31 (Dimeric phloem specific lectin PP2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G00020 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 3.7e-86
Identity = 156/230 (67.83%), Postives = 193/230 (83.91%), Query Frame = 0

Query: 1   MTTEAEAKQHLVKKLEYDHCLETILALADDQAGVEWPTFLPLCNQLSQRGISLCNGTIKF 60
           M +E EAK+ LV KL Y++CLE+ILALADD+A ++ P FLPLC+QL+  GISLC GTIKF
Sbjct: 1   MGSEEEAKKLLVNKLVYNNCLESILALADDKAALKLPVFLPLCDQLAS-GISLCGGTIKF 60

Query: 61  RINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGAFSA 120
           R+N+ TNTNRLFILPKILTI+ VNDPRYW WI+ITDHIT+VD P+LI+I KLDIRG FS+
Sbjct: 61  RLNIATNTNRLFILPKILTIYRVNDPRYWTWIHITDHITAVDGPKLISITKLDIRGKFSS 120

Query: 121 PLVTPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKPIGIFFELTVG 180
            L+T   + V +LLV+  E+  GW+TP+NV++TTPDG T  +QVSL RKPIG+FFEL VG
Sbjct: 121 RLLTQNTVYVCALLVIFYEDKSGWETPVNVSLTTPDGKTVTNQVSLERKPIGMFFELPVG 180

Query: 181 EFTLNDDGCNSTGLVKFSVTEYSNYEKRGMLIKGCIVRAKFSLGCTNPTL 231
           EFT+N+ GC  TGLV+F+VTEYSN+EKRG+LIKGC+VRAKFS GCT PT+
Sbjct: 181 EFTVNNKGCEDTGLVQFAVTEYSNHEKRGLLIKGCLVRAKFSNGCTTPTV 229

BLAST of CSPI01G34520 vs. ExPASy TrEMBL
Match: A0A1S3C9A4 (uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 OS=Cucumis melo OX=3656 GN=LOC103498272 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 3.7e-86
Identity = 156/230 (67.83%), Postives = 193/230 (83.91%), Query Frame = 0

Query: 1   MTTEAEAKQHLVKKLEYDHCLETILALADDQAGVEWPTFLPLCNQLSQRGISLCNGTIKF 60
           M +E EAK+ LV KL Y++CLE+ILALADD+A ++ P FLPLC+QL+  GISLC GTIKF
Sbjct: 1   MGSEEEAKKLLVNKLVYNNCLESILALADDKAALKLPVFLPLCDQLAS-GISLCGGTIKF 60

Query: 61  RINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGAFSA 120
           R+N+ TNTNRLFILPKILTI+ VNDPRYW WI+ITDHIT+VD P+LI+I KLDIRG FS+
Sbjct: 61  RLNIATNTNRLFILPKILTIYRVNDPRYWTWIHITDHITAVDGPKLISITKLDIRGKFSS 120

Query: 121 PLVTPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKPIGIFFELTVG 180
            L+T   + V +LLV+  E+  GW+TP+NV++TTPDG T  +QVSL RKPIG+FFEL VG
Sbjct: 121 RLLTQNTVYVCALLVIFYEDKSGWETPVNVSLTTPDGKTVTNQVSLERKPIGMFFELPVG 180

Query: 181 EFTLNDDGCNSTGLVKFSVTEYSNYEKRGMLIKGCIVRAKFSLGCTNPTL 231
           EFT+N+ GC  TGLV+F+VTEYSN+EKRG+LIKGC+VRAKFS GCT PT+
Sbjct: 181 EFTVNNKGCEDTGLVQFAVTEYSNHEKRGLLIKGCLVRAKFSNGCTTPTV 229

BLAST of CSPI01G34520 vs. ExPASy TrEMBL
Match: Q8LK97 (26 kDa phloem lectin OS=Cucumis melo OX=3656 GN=Lec26 PE=2 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.9e-29
Identity = 79/224 (35.27%), Postives = 126/224 (56.25%), Query Frame = 0

Query: 4   EAEAKQHLVKKLEYDHCLETILALADDQAGVEWPTFLPLCNQLSQRGISLCNGTIKFRIN 63
           E EA+++L  K ++ HCL  IL    +   V WP++  L  QL + GI+L NGT  +  +
Sbjct: 5   ETEARENLQIKEKFGHCLSYILPSTGNPV-VHWPSYSKLYQQLCE-GITLNNGTKIYWFD 64

Query: 64  LETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGAFSAPLV 123
            +   N  F+LPK L+I  ++D RYWKWI++      ++  +LI +  LD RG     ++
Sbjct: 65  KKGKGNGYFLLPKSLSIAWIDDRRYWKWIFVELSGKKLEVAELIRVSWLDARGKIKEYML 124

Query: 124 TPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKPIGIFFELTVGEFT 183
           +P ++  +   +LL     GW  P+N  +T PDG T  +Q SL  +P  ++F + VGEF 
Sbjct: 125 SPGIVYEVLCHLLLKPGASGWHEPINFGLTFPDGKTYVNQESLECRPRDVWFTVKVGEFK 184

Query: 184 LND-DGCNSTGLVKFSVTEYSNYEKRGMLIKGCIVRAK-FSLGC 226
           ++D  GC+ST   +FS+  +  + K  M++KG  +R K  S GC
Sbjct: 185 VDDRHGCDSTKEYEFSMYNHGGHWKTEMILKGYEIRPKQSSCGC 226

BLAST of CSPI01G34520 vs. ExPASy TrEMBL
Match: Q8LK67 (Phloem lectin OS=Cucurbita argyrosperma subsp. sororia OX=37648 GN=PP2 PE=2 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 9.3e-29
Identity = 78/222 (35.14%), Postives = 126/222 (56.76%), Query Frame = 0

Query: 4   EAEAKQHLVKKLEYDHCLETILALADDQAGVEWPTFLPLCNQLSQRGISLCNGTIKFRIN 63
           E EA++ L  +++  HCL+ IL  AD    + +P+FL L +QL   GI L  G IK+  +
Sbjct: 5   EKEAREKLGGEVKLGHCLDVILKNAD--VALHYPSFLKLYDQL-VAGILLNKGAIKYIFD 64

Query: 64  LETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGAFSAPLV 123
            ++N+N  FI  + L+I  + D RYWKW       +     +LI +  LDIRG  +  ++
Sbjct: 65  KKSNSNWYFIFARALSIAWIEDKRYWKW------GSCNKIAELIQVSWLDIRGKINESML 124

Query: 124 TPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKPIGIFFELTVGEFT 183
           +P ++  ++L V L     GW  P+N+ +  P+GS  + Q  L  KP   +FE+ + EF 
Sbjct: 125 SPNIVYEVALQVQLNSGASGWNHPMNIELKKPNGSKIERQECLLGKPKNQWFEIVI-EFK 184

Query: 184 LNDDGCNSTGLVKFSVTEYSNYEKRGMLIKGCIVRAKFSLGC 226
           +++ GC S+G ++FS  E+  + KRG+L+KG  + AK   GC
Sbjct: 185 VDNHGCGSSGEIEFSFFEHGGHWKRGLLVKGVRIGAK-GCGC 215

BLAST of CSPI01G34520 vs. NCBI nr
Match: XP_004145448.1 (lectin [Cucumis sativus] >KGN66882.1 hypothetical protein Csa_007569 [Cucumis sativus])

HSP 1 Score: 478.0 bits (1229), Expect = 4.6e-131
Identity = 233/233 (100.00%), Postives = 233/233 (100.00%), Query Frame = 0

Query: 1   MTTEAEAKQHLVKKLEYDHCLETILALADDQAGVEWPTFLPLCNQLSQRGISLCNGTIKF 60
           MTTEAEAKQHLVKKLEYDHCLETILALADDQAGVEWPTFLPLCNQLSQRGISLCNGTIKF
Sbjct: 1   MTTEAEAKQHLVKKLEYDHCLETILALADDQAGVEWPTFLPLCNQLSQRGISLCNGTIKF 60

Query: 61  RINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGAFSA 120
           RINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGAFSA
Sbjct: 61  RINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGAFSA 120

Query: 121 PLVTPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKPIGIFFELTVG 180
           PLVTPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKPIGIFFELTVG
Sbjct: 121 PLVTPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKPIGIFFELTVG 180

Query: 181 EFTLNDDGCNSTGLVKFSVTEYSNYEKRGMLIKGCIVRAKFSLGCTNPTLDRA 234
           EFTLNDDGCNSTGLVKFSVTEYSNYEKRGMLIKGCIVRAKFSLGCTNPTLDRA
Sbjct: 181 EFTLNDDGCNSTGLVKFSVTEYSNYEKRGMLIKGCIVRAKFSLGCTNPTLDRA 233

BLAST of CSPI01G34520 vs. NCBI nr
Match: XP_008459050.1 (PREDICTED: uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 [Cucumis melo] >KAA0043215.1 dimeric phloem specific lectin PP2 [Cucumis melo var. makuwa] >TYK12253.1 dimeric phloem specific lectin PP2 [Cucumis melo var. makuwa])

HSP 1 Score: 327.8 bits (839), Expect = 7.7e-86
Identity = 156/230 (67.83%), Postives = 193/230 (83.91%), Query Frame = 0

Query: 1   MTTEAEAKQHLVKKLEYDHCLETILALADDQAGVEWPTFLPLCNQLSQRGISLCNGTIKF 60
           M +E EAK+ LV KL Y++CLE+ILALADD+A ++ P FLPLC+QL+  GISLC GTIKF
Sbjct: 1   MGSEEEAKKLLVNKLVYNNCLESILALADDKAALKLPVFLPLCDQLAS-GISLCGGTIKF 60

Query: 61  RINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGAFSA 120
           R+N+ TNTNRLFILPKILTI+ VNDPRYW WI+ITDHIT+VD P+LI+I KLDIRG FS+
Sbjct: 61  RLNIATNTNRLFILPKILTIYRVNDPRYWTWIHITDHITAVDGPKLISITKLDIRGKFSS 120

Query: 121 PLVTPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKPIGIFFELTVG 180
            L+T   + V +LLV+  E+  GW+TP+NV++TTPDG T  +QVSL RKPIG+FFEL VG
Sbjct: 121 RLLTQNTVYVCALLVIFYEDKSGWETPVNVSLTTPDGKTVTNQVSLERKPIGMFFELPVG 180

Query: 181 EFTLNDDGCNSTGLVKFSVTEYSNYEKRGMLIKGCIVRAKFSLGCTNPTL 231
           EFT+N+ GC  TGLV+F+VTEYSN+EKRG+LIKGC+VRAKFS GCT PT+
Sbjct: 181 EFTVNNKGCEDTGLVQFAVTEYSNHEKRGLLIKGCLVRAKFSNGCTTPTV 229

BLAST of CSPI01G34520 vs. NCBI nr
Match: XP_038893562.1 (lectin-like [Benincasa hispida])

HSP 1 Score: 209.5 bits (532), Expect = 3.1e-50
Identity = 103/227 (45.37%), Postives = 156/227 (68.72%), Query Frame = 0

Query: 4   EAEAKQHLVKKLEYDHCLETILALADDQAGVEWPTFLPLCNQLSQRGISLCNGTIKFRIN 63
           E EA+++L  KL Y H L+ I + +DD+  ++ PTF+PL +QL   G+S   GT KFR+N
Sbjct: 5   ELEARKYLGVKLSYGHNLDCIFSHSDDK--LQNPTFVPLFDQLLD-GVSFNQGTRKFRLN 64

Query: 64  LETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGAFSAPLV 123
             TN+NR+FILPK L+I  VND RYWKWI +TD+   V+APQLI +   DI G+  A  +
Sbjct: 65  QPTNSNRVFILPKALSIAWVNDTRYWKWIIVTDNRNEVEAPQLIQVSWFDIHGSVKASWL 124

Query: 124 TPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKPIGIFFELTVGEFT 183
           + +V+ +++ LV+LTE+  GW   +N+TI  P+G T +++VSL  KP G FFE+  G+F 
Sbjct: 125 SSRVVYLVAFLVMLTEDASGWNHHVNLTIKKPNGCTVETKVSLEGKPKGEFFEVWAGDFI 184

Query: 184 LNDDGCNSTGLVKFSVTEYSNYEKRGMLIKGCIVRAKFSLGCTNPTL 231
           L++ GC  +G+V+F + E+  + KRG+++KG ++R+K S GC + TL
Sbjct: 185 LDNCGCGDSGVVEFGMYEHGGHWKRGLIVKGVVIRSKASAGCPHETL 228

BLAST of CSPI01G34520 vs. NCBI nr
Match: WP_219729244.1 (PP2 domain-containing protein, partial [Pectobacterium odoriferum] >POE05059.1 hypothetical protein BV921_23310, partial [Pectobacterium odoriferum])

HSP 1 Score: 175.3 bits (443), Expect = 6.4e-40
Identity = 84/222 (37.84%), Postives = 144/222 (64.86%), Query Frame = 0

Query: 4   EAEAKQHLVKKLEYDHCLETILALADDQAGVEWPTFLPLCNQLSQRGISLCNGTIKFRIN 63
           E EA+++L  +++  HCL  IL  AD++  + WP+++PL +QL   GISL NG  K+ +N
Sbjct: 10  EVEARKYLGLQVQLGHCLSYILPQADEK--LLWPSYIPLFDQLVY-GISLNNGAQKYYLN 69

Query: 64  LETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGAFSAPLV 123
            +TN+NR++I PK L I   +DPRYWKW  + +         LI +   D+RG+  A L+
Sbjct: 70  KQTNSNRVYIPPKSLNIAWGHDPRYWKWELLEEWDKRALLAVLIKVNWFDVRGSVKASLL 129

Query: 124 TPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKPIGIFFELTVGEFT 183
           + ++L +++ +V   E+  GW  P+N+ +  P+G   +S+V++  KP G +FE+  GE  
Sbjct: 130 SSRILYLVAFVVYFNEDAHGWHVPVNLVLKKPNGCKIESKVNIEGKPKGEYFEVDAGELI 189

Query: 184 LNDDGCNSTGLVKFSVTEYSNYEKRGMLIKGCIVRAKFSLGC 226
           L++ GC  +G+++F++ E+ ++EKRG+++KG I+R+K S GC
Sbjct: 190 LDNCGCGDSGIIEFAMYEHGSHEKRGLVMKGVILRSKASDGC 228

BLAST of CSPI01G34520 vs. NCBI nr
Match: NP_001284457.1 (protein PHLOEM PROTEIN 2-LIKE A1-like [Cucumis melo] >AAM74921.1 26 kDa phloem lectin [Cucumis melo])

HSP 1 Score: 139.4 bits (350), Expect = 3.9e-29
Identity = 79/224 (35.27%), Postives = 126/224 (56.25%), Query Frame = 0

Query: 4   EAEAKQHLVKKLEYDHCLETILALADDQAGVEWPTFLPLCNQLSQRGISLCNGTIKFRIN 63
           E EA+++L  K ++ HCL  IL    +   V WP++  L  QL + GI+L NGT  +  +
Sbjct: 5   ETEARENLQIKEKFGHCLSYILPSTGNPV-VHWPSYSKLYQQLCE-GITLNNGTKIYWFD 64

Query: 64  LETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGAFSAPLV 123
            +   N  F+LPK L+I  ++D RYWKWI++      ++  +LI +  LD RG     ++
Sbjct: 65  KKGKGNGYFLLPKSLSIAWIDDRRYWKWIFVELSGKKLEVAELIRVSWLDARGKIKEYML 124

Query: 124 TPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKPIGIFFELTVGEFT 183
           +P ++  +   +LL     GW  P+N  +T PDG T  +Q SL  +P  ++F + VGEF 
Sbjct: 125 SPGIVYEVLCHLLLKPGASGWHEPINFGLTFPDGKTYVNQESLECRPRDVWFTVKVGEFK 184

Query: 184 LND-DGCNSTGLVKFSVTEYSNYEKRGMLIKGCIVRAK-FSLGC 226
           ++D  GC+ST   +FS+  +  + K  M++KG  +R K  S GC
Sbjct: 185 VDDRHGCDSTKEYEFSMYNHGGHWKTEMILKGYEIRPKQSSCGC 226

BLAST of CSPI01G34520 vs. TAIR 10
Match: AT4G19840.1 (phloem protein 2-A1 )

HSP 1 Score: 68.9 bits (167), Expect = 6.0e-12
Identity = 49/167 (29.34%), Postives = 85/167 (50.90%), Query Frame = 0

Query: 58  IKFRINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHIT-SVDAPQLIAIQKLDIRG 117
           IK+ ++ E N+N   +  K L+I   +D  YW W    +    +V+A  L  +  LDI G
Sbjct: 83  IKYWVD-ERNSNCFMLFAKNLSITWSDDVNYWTWFTEKESPNENVEAVGLKNVCWLDITG 142

Query: 118 AFSAPLVTPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTR--QSQVSLARKPIGIF 177
            F    +TP ++  +   V L +   GW TP+N+ +  P+G  +  + +VSL   P   +
Sbjct: 143 KFDTRNLTPGIVYEVVFKVKLEDPAYGWDTPVNLKLVLPNGKEKPQEKKVSLRELPRYKW 202

Query: 178 FELTVGEFTLNDDGCNSTGLVKFSVTEY-SNYEKRGMLIKGCIVRAK 221
            ++ VGEF       ++ G + FS+ E+ +   K+G+ +KG  +R K
Sbjct: 203 VDVRVGEFVPEK---SAAGEITFSMYEHAAGVWKKGLSLKGVAIRPK 245

BLAST of CSPI01G34520 vs. TAIR 10
Match: AT1G33920.1 (phloem protein 2-A4 )

HSP 1 Score: 65.9 bits (159), Expect = 5.1e-11
Identity = 48/154 (31.17%), Postives = 77/154 (50.00%), Query Frame = 0

Query: 73  ILPKILTIFGVNDPRYWKWIYITDHITS---VDAPQLIAIQKLDIRGAFSAPLVTPKVLN 132
           I  + L+I   +   YW W+ +   I+S   VDA  L A+  LD+ G F    +T +   
Sbjct: 14  IYARDLSIAWSDKDEYWSWLPLRYDISSEKLVDAAVLEAVCWLDVNGKFDTRELTLETTY 73

Query: 133 VLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKP-IG-IFFELTVGEFTLNDD 192
            +  +V L +   GW  P+N+ +T PDG  R  + S+  K  IG  + +++ GEF  + D
Sbjct: 74  EVVYVVKLEDTASGWNIPVNLKLTLPDGKKRPQERSMCLKEHIGKRWIDISAGEFVTSPD 133

Query: 193 GCNSTGLVKFSVTE-YSNYEKRGMLIKGCIVRAK 221
              + G + FS+ E  S   KRG+ +K   +R K
Sbjct: 134 ---NAGEISFSMYETKSCCWKRGLFVKCVEIRPK 164

BLAST of CSPI01G34520 vs. TAIR 10
Match: AT2G02300.1 (phloem protein 2-B5 )

HSP 1 Score: 62.8 bits (151), Expect = 4.3e-10
Identity = 45/174 (25.86%), Postives = 83/174 (47.70%), Query Frame = 0

Query: 55  NGTIKFRINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDI 114
           NG + FR++  +    + +  K L I  V +P+YWKWI I +     + P+L+ I   DI
Sbjct: 112 NGKMSFRLDKASGKKCVMLSAKKLLISRVVNPKYWKWISIPESRFD-EVPELLNIDSFDI 171

Query: 115 RGAFSAPLVTPKVLNVLSLLVLLTENTGGWQT-PLNVTITTPDGSTRQSQVSL-ARKPIG 174
           RG  +  +++P       ++   T +  G+QT P+   +        ++ +   ++K   
Sbjct: 172 RGVLNTRIISPGTHYSAYIVYTKTSHFNGFQTSPIQAGVGFQRHGMSKTFIRFDSKKRQD 231

Query: 175 IFFELTVGEFTLNDDGCNSTGLVKFSVTEYSNYE----KRGMLIKGCIVRAKFS 223
            + E  +G+F  N+ G     L++ SV + + Y     K G++I+G   R K S
Sbjct: 232 GWMEAKIGDF-YNEGGLIGFNLIEVSVVDVARYPHMNMKSGLIIEGIEFRPKDS 283

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C0HJV29.6e-2329.95Lectin OS=Luffa acutangula OX=56866 PE=1 SV=1[more]
O818658.4e-1129.34Protein PHLOEM PROTEIN 2-LIKE A1 OS=Arabidopsis thaliana OX=3702 GN=PP2A1 PE=2 S... [more]
Q9C8U97.1e-1031.17Uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 OS=Arabidopsis thaliana OX=3702... [more]
P0DSP54.6e-0926.85Lectin OS=Coccinia grandis OX=387127 PE=1 SV=1[more]
Q9ZVR16.1e-0925.86F-box protein PP2-B5 OS=Arabidopsis thaliana OX=3702 GN=PP2B5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0M1832.2e-131100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G703060 PE=4 SV=1[more]
A0A5D3CK313.7e-8667.83Dimeric phloem specific lectin PP2 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3C9A43.7e-8667.83uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 OS=Cucumis melo OX=3656 GN=LOC1... [more]
Q8LK971.9e-2935.2726 kDa phloem lectin OS=Cucumis melo OX=3656 GN=Lec26 PE=2 SV=1[more]
Q8LK679.3e-2935.14Phloem lectin OS=Cucurbita argyrosperma subsp. sororia OX=37648 GN=PP2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004145448.14.6e-131100.00lectin [Cucumis sativus] >KGN66882.1 hypothetical protein Csa_007569 [Cucumis sa... [more]
XP_008459050.17.7e-8667.83PREDICTED: uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 [Cucumis melo] >KAA0... [more]
XP_038893562.13.1e-5045.37lectin-like [Benincasa hispida][more]
WP_219729244.16.4e-4037.84PP2 domain-containing protein, partial [Pectobacterium odoriferum] >POE05059.1 h... [more]
NP_001284457.13.9e-2935.27protein PHLOEM PROTEIN 2-LIKE A1-like [Cucumis melo] >AAM74921.1 26 kDa phloem l... [more]
Match NameE-valueIdentityDescription
AT4G19840.16.0e-1229.34phloem protein 2-A1 [more]
AT1G33920.15.1e-1131.17phloem protein 2-A4 [more]
AT2G02300.14.3e-1025.86phloem protein 2-B5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025886Phloem protein 2-likePFAMPF14299PP2coord: 72..218
e-value: 3.1E-16
score: 59.7
NoneNo IPR availablePANTHERPTHR32278FAMILY NOT NAMEDcoord: 32..218
NoneNo IPR availablePANTHERPTHR32278:SF42PROTEIN PHLOEM PROTEIN 2-LIKE A2coord: 32..218

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G34520.1CSPI01G34520.1mRNA