CSPI01G34240 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G34240
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionSubtilisin-like protease
LocationChr1: 29123072 .. 29125161 (+)
RNA-Seq ExpressionCSPI01G34240
SyntenyCSPI01G34240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTAGAGAATTTAAAGGGAAAGCAGGGATTGGAGAAGGAAGAATTGCTTCATTTGGGAACCAAGCACCCACAGTTAGCAGATTTTCATCTAGAGGGCCAGATTACATAAACATAAATAGGAGTTTAGCTGATGTTCTAAAGCCAGATATTCTTGCACCTGGTCACCAAATTTGGGCTGCTTGGAGCCCTCTCAGTGCCTCAGAACCACTATTAAAGGGTCAGCTTTTCATGAACTTATTGTTCTTTCAGTTTCATCAATTTTTCCCTTTTACATTTGTCATTCAATCTATATGAATTTTAAGCAATTCTTGTTTTCGTCTCTTTAGGTTTTAAAACCCCAGTGCTAAAAAAAATATATTTGTTTTTAGTTTTCTCTGTATGAAATTTATATGTACTGTTTTTCTCTTAAATTTTATATTATGGTTCTTACATTTATAAAACAAACAATTGGGTTCTCAGTAAACTTTTACAATTACATGACCCTACTCTTTCTTAATTTCTTATGATCTTTTGCATCTCTCTCAAGTAATATCTAGTTGAACTTCCTAGCCAAATTCTATAAACAAGAAAAATTTCTTAAAAAGCTAATCTTTCTAGTTTTCAGAACTTTAATTTGAAACCTGGTTTTTGAAAACAACATATAGATAACAAAATTACAAAAAGCTTAGAGGTGGAAGAAGTATCTACCGGCATGCTTTTAATTCCTAAAAGTTAAAAATTGAAAACAAAGTTATCAAATAAATTTTTTTTTCTAGTCTTTTAAATTTTGTTTTGACTTTTACAAATATCAATGAAAAGTGAATAACAAAACATAAACAAAGAAACATAGTGAAACTAGAGTTTATTAGTTTAATTTTTTAAAAAAAAAAAAAATTAGTAAAGACGATCTAGATAAATTTCACCTTTATAAGTCCCAATCAACTCAAGAAATTAGAAAGTCATATTTATGTCATATCAAAAGGGCACAAGAGAAGTTGATTGAAACATGAGTGTTGAATTATATATATACAGGATACCATTTTGCACTGATGTCTGGAACAAGTATGGCAGCACCTCATATTGTGGGAATAGCAGCACTTATCAAGCAAAAATATCCTTCTTGGACTCCTTCCATGATAGCTTCTGCTATGTCCACAACTGCTACAAAGTATGATATGAATGGAGACCTTATTCAGGCTGAGGGATTCAACCTTCATGCTTTATATCCTTCTACTCCCTTCGATTTCGGCGCTGGGCTCGTTTCTCCGTCCAACGCTTTGGACCCCGGACTCGTCTTCCCAACAGGTAAATTCTAATGATACATAACTCTCTACCTTTTCATTATTTGGACAACTGTGTGTTTGTTGAATATCTCAAAGCTACTTAATAACGCTTTGAAATGTTTGTGGTAGTAAAAAAGAACTCACCAATTAATGTTCCCTTGAAGTCTAACCGTGCTTGTGTTAGGAATACCACTTAACATGAAAGCTTAGAGGAATACAAAGAAACATCAAGATAGTAATATATTACAATATTAAAGAAAAAACTAAACTAATATGGCTGATATCTAAGAATTGTAATTTAACAACTCTAAACTAGAAATACAAATATTGCAAATTTCTTTTGCCAACAGATTCAGTCTAAGCCTATTCAAACATCAAGAGAACTAACCATATCTTCTTGAAACAGAATATGAAGACAATATAAACTTCTTGTGCTCTTTACCTGGGGTTGATCCTGCCGTCGTCAAAAGCGCCACCGGAGGACAATGTAATGCCTCTATCTCACACTCACACCCTGCAGATCTGAACCTTCCTTCAATCACAATATCATCACTGGTTGGGCATCAGGTGGTGCAGAGAAGAGTGAAGAATGTAGGAGGAAAAGTGGAGACATATGTATGGTCAGTGATCCCACCAAACGGGACAACAGTCAACATCAATCCGCCGGTGTTCACTGTAGCTGCCGAAGAAGTCCAAAACTTGGAAATACAGATCATTGCCACACATAAAACGGATCATTTTACCTTTGGGGAGATTATTTTGACAGGAAGTTTAAACCATATTGCAAGAATCCCTTTATCAATTTTGGTTGTTTCTGCATCCTGA

mRNA sequence

ATGGTTAGAGAATTTAAAGGGAAAGCAGGGATTGGAGAAGGAAGAATTGCTTCATTTGGGAACCAAGCACCCACAGTTAGCAGATTTTCATCTAGAGGGCCAGATTACATAAACATAAATAGGAGTTTAGCTGATGTTCTAAAGCCAGATATTCTTGCACCTGGTCACCAAATTTGGGCTGCTTGGAGCCCTCTCAGTGCCTCAGAACCACTATTAAAGGGATACCATTTTGCACTGATGTCTGGAACAAGTATGGCAGCACCTCATATTGTGGGAATAGCAGCACTTATCAAGCAAAAATATCCTTCTTGGACTCCTTCCATGATAGCTTCTGCTATGTCCACAACTGCTACAAAGTATGATATGAATGGAGACCTTATTCAGGCTGAGGGATTCAACCTTCATGCTTTATATCCTTCTACTCCCTTCGATTTCGGCGCTGGGCTCGTTTCTCCGTCCAACGCTTTGGACCCCGGACTCGTCTTCCCAACAGAATATGAAGACAATATAAACTTCTTGTGCTCTTTACCTGGGGTTGATCCTGCCGTCGTCAAAAGCGCCACCGGAGGACAATGTAATGCCTCTATCTCACACTCACACCCTGCAGATCTGAACCTTCCTTCAATCACAATATCATCACTGGTTGGGCATCAGGTGGTGCAGAGAAGAGTGAAGAATGTAGGAGGAAAAGTGGAGACATATGTATGGTCAGTGATCCCACCAAACGGGACAACAGTCAACATCAATCCGCCGGTGTTCACTGTAGCTGCCGAAGAAGTCCAAAACTTGGAAATACAGATCATTGCCACACATAAAACGGATCATTTTACCTTTGGGGAGATTATTTTGACAGGAAGTTTAAACCATATTGCAAGAATCCCTTTATCAATTTTGGTTGTTTCTGCATCCTGA

Coding sequence (CDS)

ATGGTTAGAGAATTTAAAGGGAAAGCAGGGATTGGAGAAGGAAGAATTGCTTCATTTGGGAACCAAGCACCCACAGTTAGCAGATTTTCATCTAGAGGGCCAGATTACATAAACATAAATAGGAGTTTAGCTGATGTTCTAAAGCCAGATATTCTTGCACCTGGTCACCAAATTTGGGCTGCTTGGAGCCCTCTCAGTGCCTCAGAACCACTATTAAAGGGATACCATTTTGCACTGATGTCTGGAACAAGTATGGCAGCACCTCATATTGTGGGAATAGCAGCACTTATCAAGCAAAAATATCCTTCTTGGACTCCTTCCATGATAGCTTCTGCTATGTCCACAACTGCTACAAAGTATGATATGAATGGAGACCTTATTCAGGCTGAGGGATTCAACCTTCATGCTTTATATCCTTCTACTCCCTTCGATTTCGGCGCTGGGCTCGTTTCTCCGTCCAACGCTTTGGACCCCGGACTCGTCTTCCCAACAGAATATGAAGACAATATAAACTTCTTGTGCTCTTTACCTGGGGTTGATCCTGCCGTCGTCAAAAGCGCCACCGGAGGACAATGTAATGCCTCTATCTCACACTCACACCCTGCAGATCTGAACCTTCCTTCAATCACAATATCATCACTGGTTGGGCATCAGGTGGTGCAGAGAAGAGTGAAGAATGTAGGAGGAAAAGTGGAGACATATGTATGGTCAGTGATCCCACCAAACGGGACAACAGTCAACATCAATCCGCCGGTGTTCACTGTAGCTGCCGAAGAAGTCCAAAACTTGGAAATACAGATCATTGCCACACATAAAACGGATCATTTTACCTTTGGGGAGATTATTTTGACAGGAAGTTTAAACCATATTGCAAGAATCCCTTTATCAATTTTGGTTGTTTCTGCATCCTGA

Protein sequence

MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAAWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIPPNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVVSAS*
Homology
[Close]
BLAST of CSPI01G34240 vs. ExPASy Swiss-Prot
Match: F4HYR6 (Subtilisin-like protease SBT2.4 OS=Arabidopsis thaliana OX=3702 GN=SBT2.4 PE=1 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.2e-94
Identity = 173/297 (58.25%), Postives = 218/297 (73.40%), Query Frame = 0 

Query: 4   EFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAAWS 63
           +F  +A IGEGR + F  +AP VSRFSSRGP +I+  RS  DVLKPDILAPGHQIW AWS
Sbjct: 538 QFGARARIGEGRNSVFAGKAPVVSRFSSRGPAFIDATRSPLDVLKPDILAPGHQIWGAWS 597

Query: 64  PLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYDMN 123
             SA +P+L G  FA++SGTSMA PHI GI ALIKQ  PSWTP+MIASA+STTA +YD N
Sbjct: 598 LPSAFDPILTGRSFAILSGTSMATPHIAGIGALIKQLNPSWTPAMIASAISTTANEYDSN 657

Query: 124 GDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVDPAV 183
           G++I AE + L  L+PS  FD GAG V+P+ ALDPGLV P  +ED I+FLCSLP + PA 
Sbjct: 658 GEIISAEYYELSRLFPSNHFDHGAGHVNPARALDPGLVLPAGFEDYISFLCSLPNISPAT 717

Query: 184 VKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIPPNG 243
           ++ ATG  C  ++  SHPA+LN PS+TIS+L    VV+R  ++V  K ETY+ SV+PPNG
Sbjct: 718 IRDATGVLCTTTL--SHPANLNHPSVTISALKESLVVRRSFQDVSNKTETYLGSVLPPNG 777

Query: 244 TTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 301
           TTV + P  FTV  ++ Q+L+I+   T   + FTFGE++LTGSLNHI RIPLS+  +
Sbjct: 778 TTVRLTPTWFTVPPQKTQDLDIEFNVTQVLNKFTFGEVVLTGSLNHIIRIPLSVKTI 832

[Close]
BLAST of CSPI01G34240 vs. ExPASy Swiss-Prot
Match: O64481 (Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 2.0e-65
Identity = 131/300 (43.67%), Postives = 180/300 (60.00%), Query Frame = 0 

Query: 2   VREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAA 61
           V+ FK +  IG+G        AP V+ FS+RGP+  + +   AD+LKPDILAPG+ IWAA
Sbjct: 514 VKSFKAEGSIGDGLAPVLHKSAPQVALFSARGPNTKDFSFQDADLLKPDILAPGYLIWAA 573

Query: 62  WSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYD 121
           W P    EP   G  FAL+SGTSMAAPHI GIAAL+KQK+P W+P+ I SA+ TT+T  D
Sbjct: 574 WCPNGTDEPNYVGEGFALISGTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTSTVID 633

Query: 122 MNGDLIQAEGFN---LHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPG 181
             G L+QA+ ++      L  +TPFD+G+G V+PS ALDPGL+F   YED + FLC+ PG
Sbjct: 634 RAGRLLQAQQYSDTEAVTLVKATPFDYGSGHVNPSAALDPGLIFDAGYEDYLGFLCTTPG 693

Query: 182 VDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSV 241
           +    +++ T   CN  +   HP++ N PSI +S LVG Q V R+V NV    ETY  + 
Sbjct: 694 ISAHEIRNYTNTACNYDM--KHPSNFNAPSIAVSHLVGTQTVTRKVTNVAEVEETYTITA 753

Query: 242 IPPNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSIL 299
                  + +NPP  T+     +   + +     +  ++FGE+ L GS  H  RIP+  L
Sbjct: 754 RMQPSIAIEVNPPAMTLRPGATRTFSVTMTVRSVSGVYSFGEVKLKGSRGHKVRIPVVAL 811

[Close]
BLAST of CSPI01G34240 vs. ExPASy Swiss-Prot
Match: Q9FI12 (Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana OX=3702 GN=SBT2.3 PE=2 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 1.9e-63
Identity = 137/302 (45.36%), Postives = 189/302 (62.58%), Query Frame = 0 

Query: 5   FKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAAWSP 64
           F   A I  G  A+F N+AP V  +S+RGPD  + + + ADVLKP+++APG+ IW AWS 
Sbjct: 540 FGAVAAIEGGLNANFSNRAPKVMYYSARGPDPEDNSFNDADVLKPNLVAPGNSIWGAWSS 599

Query: 65  LSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYDMNG 124
            S      +G  FA+MSGTSMAAPH+ G+AALIKQ YP +TPS I+SA+STTA   D  G
Sbjct: 600 ASTDSTEFEGEKFAMMSGTSMAAPHVAGVAALIKQSYPQFTPSTISSALSTTALLNDNKG 659

Query: 125 DLIQAEGFNLH---ALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVDP 184
             I A+    +   +LY +TP D G+G V+ + ALDPGLVF T +ED I+FLC + G D 
Sbjct: 660 SPIMAQRTYSNPDQSLYTATPSDMGSGFVNATAALDPGLVFDTSFEDYISFLCGINGSD- 719

Query: 185 AVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIPP 244
            VV + TG +C A+ +     DLNLPSIT+S+L G Q  QR ++N+ G  ETY     PP
Sbjct: 720 TVVFNYTGFRCPANNTPVSGFDLNLPSITVSTLSGTQTFQRSMRNIAGN-ETYNVGWSPP 779

Query: 245 NGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVVS 304
            G ++ ++P  F++A  E Q L + +  T  +   +FG I L G+  HI  IP++++   
Sbjct: 780 YGVSMKVSPTQFSIAMGENQVLSVTLTVTKNSSSSSFGRIGLFGNTGHIVNIPVTVIAKI 839

[Close]
BLAST of CSPI01G34240 vs. ExPASy Swiss-Prot
Match: Q9SZV5 (Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 5.5e-63
Identity = 130/297 (43.77%), Postives = 178/297 (59.93%), Query Frame = 0 

Query: 2   VREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAA 61
           V++FK +  IG+G        AP V+ FS+RGP+  + +   AD+LKPDILAPG  IW+A
Sbjct: 515 VKDFKAEGSIGDGLEPILHKSAPEVALFSARGPNTKDFSFQDADLLKPDILAPGSLIWSA 574

Query: 62  WSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYD 121
           WS     E    G  FAL+SGTSMAAPHI GIAAL+KQK+P W+P+ I SA+ TT+T  D
Sbjct: 575 WSANGTDEANYIGEGFALISGTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTSTVID 634

Query: 122 MNGDLIQAEGFN---LHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPG 181
             G  +QA+ ++      L  +TPFD+G+G V+PS ALDPGL+F   YED I FLC+ PG
Sbjct: 635 RAGRPLQAQQYSETETVTLVKATPFDYGSGHVNPSAALDPGLIFDAGYEDYIGFLCTTPG 694

Query: 182 VDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSV 241
           +D   +K+ T   CN  +   HP++ N PSI IS LV  Q V RRV NV  + ETY  + 
Sbjct: 695 IDAHEIKNFTNTPCNFKM--VHPSNFNTPSIAISHLVRTQTVTRRVTNVAEEEETYTITS 754

Query: 242 IPPNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPL 296
                  + ++PP  TV A   +   + +     T  ++FG++ L GS  H   +P+
Sbjct: 755 RMEPAIAIEVSPPAMTVRAGASRTFSVTLTVRSVTGAYSFGQVTLKGSRGHKVTLPV 809

[Close]
BLAST of CSPI01G34240 vs. ExPASy Swiss-Prot
Match: Q9SUN6 (Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana OX=3702 GN=SBT2.2 PE=3 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.2e-62
Identity = 135/305 (44.26%), Postives = 193/305 (63.28%), Query Frame = 0 

Query: 5   FKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAAWSP 64
           F   A I  G+ A+F N+AP +  +S+RGPD  +   + AD+LKP+++APG+ IW AWS 
Sbjct: 555 FGAVAAIAGGQNANFSNRAPKIMYYSARGPDPQDSLFNDADILKPNLVAPGNSIWGAWSS 614

Query: 65  LSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYDMNG 124
            +      +G  FA+MSGTSMAAPH+ G+AAL+KQK+  ++PS IASA+STT+  +D  G
Sbjct: 615 AATESTEFEGESFAMMSGTSMAAPHVAGVAALVKQKFRKFSPSAIASALSTTSVLFDNKG 674

Query: 125 DLIQAEGFNLH---ALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVDP 184
           + I A+    +    + P+TPFD G G V+ + ALDPGL+F T +ED ++FLC + G  P
Sbjct: 675 EAIMAQRAYANPDQTISPATPFDMGNGFVNATAALDPGLIFDTSFEDYMSFLCGINGSAP 734

Query: 185 AVVKSATGGQC---NASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSV 244
            VV + TG  C   NA+IS S   DLNLPSIT+S L   + VQR + N+ G  ETY  S+
Sbjct: 735 -VVFNYTGTNCLRNNATISGS---DLNLPSITVSKLNNTRTVQRLMTNIAGN-ETYTVSL 794

Query: 245 IPPNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSIL 304
           I P    +N++P  F++A+ E + L + + A   +   +FG I L G+  HI RIP+S+ 
Sbjct: 795 ITPFDVLINVSPTQFSIASGETKLLSVILTAKRNSSISSFGGIKLLGNAGHIVRIPVSVT 854

[Close]
BLAST of CSPI01G34240 vs. ExPASy TrEMBL
Match: A0A0A0LYA0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G701260 PE=3 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 1.1e-170
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0 

Query: 1   MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 60
           MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA
Sbjct: 1   MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 60

Query: 61  AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 120
           AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY
Sbjct: 61  AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 120

Query: 121 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 180
           DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD
Sbjct: 121 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 180

Query: 181 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 240
           PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP
Sbjct: 181 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 240

Query: 241 PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 300
           PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV
Sbjct: 241 PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 300

Query: 301 SAS 304
           SAS
Sbjct: 301 SAS 303

[Close]
BLAST of CSPI01G34240 vs. ExPASy TrEMBL
Match: A0A1S3C9G9 (LOW QUALITY PROTEIN: subtilisin-like protease SBT2.4 OS=Cucumis melo OX=3656 GN=LOC103498334 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 6.4e-163
Identity = 291/302 (96.36%), Postives = 295/302 (97.68%), Query Frame = 0 

Query: 1   MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 60
           MVREFKGKAGIGEGRIASFG +APTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA
Sbjct: 494 MVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 553

Query: 61  AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 120
           AWSPLSASEPLLKGY FALMSGTSMAAPHIVG+AALIKQKYPSWTPSMIASAMSTTATKY
Sbjct: 554 AWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPSMIASAMSTTATKY 613

Query: 121 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 180
           DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSP+NALDPGLVFPTEYED INFLCSLPGVD
Sbjct: 614 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYEDYINFLCSLPGVD 673

Query: 181 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 240
           PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVV+RRVKNVGGKVETYV SVIP
Sbjct: 674 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNVGGKVETYVCSVIP 733

Query: 241 PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 300
           PNGTTVNI PP FTVAAEEVQNLEIQIIATHKTDHFTFGEI LTGSLNHIARIPLSILVV
Sbjct: 734 PNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGEIFLTGSLNHIARIPLSILVV 793

Query: 301 SA 303
           SA
Sbjct: 794 SA 795

[Close]
BLAST of CSPI01G34240 vs. ExPASy TrEMBL
Match: A0A5A7TIJ4 (Subtilisin-like protease SBT2.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold110G00140 PE=3 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 8.4e-155
Identity = 280/302 (92.72%), Postives = 287/302 (95.03%), Query Frame = 0 

Query: 1   MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 60
           MVREFKGKAGIGEGRIASFG +APTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA
Sbjct: 466 MVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 525

Query: 61  AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 120
           AWSPLSASEPLLKG  F      ++ +PHIVG+AALIKQKYPSWTPSMIASAMSTTATKY
Sbjct: 526 AWSPLSASEPLLKGQLF-----MNLYSPHIVGVAALIKQKYPSWTPSMIASAMSTTATKY 585

Query: 121 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 180
           DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSP+NALDPGLVFPTEYED INFLCSLPGVD
Sbjct: 586 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYEDYINFLCSLPGVD 645

Query: 181 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 240
           PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVV+RRVKNVGGKVETYV SVIP
Sbjct: 646 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNVGGKVETYVCSVIP 705

Query: 241 PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 300
           PNGTTVNI PP FTVAAEEVQNLEIQIIATHKTDHFTFGEI LTGSLNHIARIPLSILVV
Sbjct: 706 PNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGEIFLTGSLNHIARIPLSILVV 762

Query: 301 SA 303
           SA
Sbjct: 766 SA 762

[Close]
BLAST of CSPI01G34240 vs. ExPASy TrEMBL
Match: A0A5D3CPQ5 (Subtilisin-like protease SBT2.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G00770 PE=4 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 2.7e-153
Identity = 279/302 (92.38%), Postives = 283/302 (93.71%), Query Frame = 0 

Query: 1   MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 60
           MVREFKGKAGIGEGRIASFG +APTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA
Sbjct: 512 MVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 571

Query: 61  AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 120
           AWSPLSASEPLLK             APHIVG+AALIKQKYPSWTPSMIASAMSTTATKY
Sbjct: 572 AWSPLSASEPLLK-------------APHIVGVAALIKQKYPSWTPSMIASAMSTTATKY 631

Query: 121 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 180
           DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSP+NALDPGLVFPTEYED INFLCSLPGVD
Sbjct: 632 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYEDYINFLCSLPGVD 691

Query: 181 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 240
           PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVV+RRVKNVGGKVETYV SVIP
Sbjct: 692 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNVGGKVETYVCSVIP 751

Query: 241 PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 300
           PNGTTVNI PP FTVAAEEVQNLEIQIIATHKTDHFTFGEI LTGSLNHIARIPLSILVV
Sbjct: 752 PNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGEIFLTGSLNHIARIPLSILVV 800

Query: 301 SA 303
           SA
Sbjct: 812 SA 800

[Close]
BLAST of CSPI01G34240 vs. ExPASy TrEMBL
Match: A0A6J1CM39 (subtilisin-like protease SBT2.4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012715 PE=3 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.1e-146
Identity = 258/303 (85.15%), Postives = 276/303 (91.09%), Query Frame = 0 

Query: 1   MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 60
           MV  FKGKA IGEGR ASFG QAPTVSRFSSRGPDY+N NR+ ADVLKPDILAPGHQ+WA
Sbjct: 521 MVTNFKGKAAIGEGRFASFGGQAPTVSRFSSRGPDYMNSNRTAADVLKPDILAPGHQVWA 580

Query: 61  AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 120
           AWSPLSASEPLLKGYHFAL+SGTSMAAPHIVGIAALIKQK PSWTPSMIASAMSTTATKY
Sbjct: 581 AWSPLSASEPLLKGYHFALLSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKY 640

Query: 121 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 180
           DMNG+LIQAEG+ +H+LYPSTPFD GAGLVSP+NALDPGLVFP+EYED INFLCSLPGVD
Sbjct: 641 DMNGELIQAEGYGIHSLYPSTPFDLGAGLVSPTNALDPGLVFPSEYEDYINFLCSLPGVD 700

Query: 181 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 240
           PAV+KS TG  CNAS+S  HPADLNLPS+TISSLVG QVVQRRVKNVGGKVETYVWSVIP
Sbjct: 701 PAVIKSTTGRHCNASLSQPHPADLNLPSLTISSLVGQQVVQRRVKNVGGKVETYVWSVIP 760

Query: 241 PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 300
           PNGTTV+I PP F +A E VQNLEIQI  THK DHF+FGEI+LTGSLNHIARIPLS+L V
Sbjct: 761 PNGTTVDIXPPWFAIAPEGVQNLEIQINVTHKMDHFSFGEIVLTGSLNHIARIPLSVLAV 820

Query: 301 SAS 304
           S S
Sbjct: 821 STS 823

[Close]
BLAST of CSPI01G34240 vs. NCBI nr
Match: XP_004145469.3 (subtilisin-like protease SBT2.4 [Cucumis sativus])

HSP 1 Score: 609.0 bits (1569), Expect = 2.2e-170
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0 

Query: 1   MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 60
           MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA
Sbjct: 494 MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 553

Query: 61  AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 120
           AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY
Sbjct: 554 AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 613

Query: 121 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 180
           DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD
Sbjct: 614 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 673

Query: 181 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 240
           PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP
Sbjct: 674 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 733

Query: 241 PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 300
           PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV
Sbjct: 734 PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 793

Query: 301 SAS 304
           SAS
Sbjct: 794 SAS 796

[Close]
BLAST of CSPI01G34240 vs. NCBI nr
Match: KAE8653651.1 (hypothetical protein Csa_006839 [Cucumis sativus])

HSP 1 Score: 609.0 bits (1569), Expect = 2.2e-170
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0 

Query: 1    MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 60
            MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA
Sbjct: 759  MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 818

Query: 61   AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 120
            AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY
Sbjct: 819  AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 878

Query: 121  DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 180
            DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD
Sbjct: 879  DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 938

Query: 181  PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 240
            PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP
Sbjct: 939  PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 998

Query: 241  PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 300
            PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV
Sbjct: 999  PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 1058

Query: 301  SAS 304
            SAS
Sbjct: 1059 SAS 1061

[Close]
BLAST of CSPI01G34240 vs. NCBI nr
Match: XP_008459133.1 (PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease SBT2.4, partial [Cucumis melo])

HSP 1 Score: 583.2 bits (1502), Expect = 1.3e-162
Identity = 291/302 (96.36%), Postives = 295/302 (97.68%), Query Frame = 0 

Query: 1   MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 60
           MVREFKGKAGIGEGRIASFG +APTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA
Sbjct: 494 MVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 553

Query: 61  AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 120
           AWSPLSASEPLLKGY FALMSGTSMAAPHIVG+AALIKQKYPSWTPSMIASAMSTTATKY
Sbjct: 554 AWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPSMIASAMSTTATKY 613

Query: 121 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 180
           DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSP+NALDPGLVFPTEYED INFLCSLPGVD
Sbjct: 614 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYEDYINFLCSLPGVD 673

Query: 181 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 240
           PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVV+RRVKNVGGKVETYV SVIP
Sbjct: 674 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNVGGKVETYVCSVIP 733

Query: 241 PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 300
           PNGTTVNI PP FTVAAEEVQNLEIQIIATHKTDHFTFGEI LTGSLNHIARIPLSILVV
Sbjct: 734 PNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGEIFLTGSLNHIARIPLSILVV 793

Query: 301 SA 303
           SA
Sbjct: 794 SA 795

[Close]
BLAST of CSPI01G34240 vs. NCBI nr
Match: XP_038895834.1 (subtilisin-like protease SBT2.4 [Benincasa hispida])

HSP 1 Score: 556.6 bits (1433), Expect = 1.3e-154
Identity = 272/303 (89.77%), Postives = 285/303 (94.06%), Query Frame = 0 

Query: 1   MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 60
           MV E KGKA IGEGR+ASFG QAPTVSRFSSRGPDYININR++ADVLKPDILAPGHQIWA
Sbjct: 452 MVSEIKGKAAIGEGRVASFGRQAPTVSRFSSRGPDYININRTVADVLKPDILAPGHQIWA 511

Query: 61  AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 120
           AWSPLSASEPLLKGYHFAL+SGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY
Sbjct: 512 AWSPLSASEPLLKGYHFALLSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 571

Query: 121 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 180
           DMNGDLIQAEGFNLH LYPSTPFDFGAGLVSP++ALDPGLVFP+EY++ INFLCSLPGVD
Sbjct: 572 DMNGDLIQAEGFNLHTLYPSTPFDFGAGLVSPTHALDPGLVFPSEYDEYINFLCSLPGVD 631

Query: 181 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 240
           PAV+KSATGGQCN  +S  HPADLNLPS+TISSLVGHQVVQRRVKNVG KVETYVWSVIP
Sbjct: 632 PAVIKSATGGQCNTCVSQPHPADLNLPSVTISSLVGHQVVQRRVKNVGSKVETYVWSVIP 691

Query: 241 PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 300
           PNGTTVNINPP FTVA EEVQNLEIQI  THK DHFTFGEIILTGSLNHIARIPLS+  V
Sbjct: 692 PNGTTVNINPPRFTVAPEEVQNLEIQINVTHKMDHFTFGEIILTGSLNHIARIPLSVKAV 751

Query: 301 SAS 304
           SAS
Sbjct: 752 SAS 754

[Close]
BLAST of CSPI01G34240 vs. NCBI nr
Match: KAA0043154.1 (subtilisin-like protease SBT2.4 [Cucumis melo var. makuwa])

HSP 1 Score: 556.2 bits (1432), Expect = 1.7e-154
Identity = 280/302 (92.72%), Postives = 287/302 (95.03%), Query Frame = 0 

Query: 1   MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 60
           MVREFKGKAGIGEGRIASFG +APTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA
Sbjct: 466 MVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 525

Query: 61  AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 120
           AWSPLSASEPLLKG  F      ++ +PHIVG+AALIKQKYPSWTPSMIASAMSTTATKY
Sbjct: 526 AWSPLSASEPLLKGQLF-----MNLYSPHIVGVAALIKQKYPSWTPSMIASAMSTTATKY 585

Query: 121 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 180
           DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSP+NALDPGLVFPTEYED INFLCSLPGVD
Sbjct: 586 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYEDYINFLCSLPGVD 645

Query: 181 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 240
           PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVV+RRVKNVGGKVETYV SVIP
Sbjct: 646 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNVGGKVETYVCSVIP 705

Query: 241 PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 300
           PNGTTVNI PP FTVAAEEVQNLEIQIIATHKTDHFTFGEI LTGSLNHIARIPLSILVV
Sbjct: 706 PNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGEIFLTGSLNHIARIPLSILVV 762

Query: 301 SA 303
           SA
Sbjct: 766 SA 762

[Close]
BLAST of CSPI01G34240 vs. TAIR 10
Match: AT1G62340.1 (PA-domain containing subtilase family protein )

HSP 1 Score: 347.8 bits (891), Expect = 8.7e-96
Identity = 173/297 (58.25%), Postives = 218/297 (73.40%), Query Frame = 0 

Query: 4   EFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAAWS 63
           +F  +A IGEGR + F  +AP VSRFSSRGP +I+  RS  DVLKPDILAPGHQIW AWS
Sbjct: 538 QFGARARIGEGRNSVFAGKAPVVSRFSSRGPAFIDATRSPLDVLKPDILAPGHQIWGAWS 597

Query: 64  PLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYDMN 123
             SA +P+L G  FA++SGTSMA PHI GI ALIKQ  PSWTP+MIASA+STTA +YD N
Sbjct: 598 LPSAFDPILTGRSFAILSGTSMATPHIAGIGALIKQLNPSWTPAMIASAISTTANEYDSN 657

Query: 124 GDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVDPAV 183
           G++I AE + L  L+PS  FD GAG V+P+ ALDPGLV P  +ED I+FLCSLP + PA 
Sbjct: 658 GEIISAEYYELSRLFPSNHFDHGAGHVNPARALDPGLVLPAGFEDYISFLCSLPNISPAT 717

Query: 184 VKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIPPNG 243
           ++ ATG  C  ++  SHPA+LN PS+TIS+L    VV+R  ++V  K ETY+ SV+PPNG
Sbjct: 718 IRDATGVLCTTTL--SHPANLNHPSVTISALKESLVVRRSFQDVSNKTETYLGSVLPPNG 777

Query: 244 TTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 301
           TTV + P  FTV  ++ Q+L+I+   T   + FTFGE++LTGSLNHI RIPLS+  +
Sbjct: 778 TTVRLTPTWFTVPPQKTQDLDIEFNVTQVLNKFTFGEVVLTGSLNHIIRIPLSVKTI 832

[Close]
BLAST of CSPI01G34240 vs. TAIR 10
Match: AT2G19170.1 (subtilisin-like serine protease 3 )

HSP 1 Score: 250.8 bits (639), Expect = 1.4e-66
Identity = 131/300 (43.67%), Postives = 180/300 (60.00%), Query Frame = 0 

Query: 2   VREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAA 61
           V+ FK +  IG+G        AP V+ FS+RGP+  + +   AD+LKPDILAPG+ IWAA
Sbjct: 514 VKSFKAEGSIGDGLAPVLHKSAPQVALFSARGPNTKDFSFQDADLLKPDILAPGYLIWAA 573

Query: 62  WSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYD 121
           W P    EP   G  FAL+SGTSMAAPHI GIAAL+KQK+P W+P+ I SA+ TT+T  D
Sbjct: 574 WCPNGTDEPNYVGEGFALISGTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTSTVID 633

Query: 122 MNGDLIQAEGFN---LHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPG 181
             G L+QA+ ++      L  +TPFD+G+G V+PS ALDPGL+F   YED + FLC+ PG
Sbjct: 634 RAGRLLQAQQYSDTEAVTLVKATPFDYGSGHVNPSAALDPGLIFDAGYEDYLGFLCTTPG 693

Query: 182 VDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSV 241
           +    +++ T   CN  +   HP++ N PSI +S LVG Q V R+V NV    ETY  + 
Sbjct: 694 ISAHEIRNYTNTACNYDM--KHPSNFNAPSIAVSHLVGTQTVTRKVTNVAEVEETYTITA 753

Query: 242 IPPNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSIL 299
                  + +NPP  T+     +   + +     +  ++FGE+ L GS  H  RIP+  L
Sbjct: 754 RMQPSIAIEVNPPAMTLRPGATRTFSVTMTVRSVSGVYSFGEVKLKGSRGHKVRIPVVAL 811

[Close]
BLAST of CSPI01G34240 vs. TAIR 10
Match: AT5G44530.1 (Subtilase family protein )

HSP 1 Score: 244.2 bits (622), Expect = 1.4e-64
Identity = 137/302 (45.36%), Postives = 189/302 (62.58%), Query Frame = 0 

Query: 5   FKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAAWSP 64
           F   A I  G  A+F N+AP V  +S+RGPD  + + + ADVLKP+++APG+ IW AWS 
Sbjct: 540 FGAVAAIEGGLNANFSNRAPKVMYYSARGPDPEDNSFNDADVLKPNLVAPGNSIWGAWSS 599

Query: 65  LSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYDMNG 124
            S      +G  FA+MSGTSMAAPH+ G+AALIKQ YP +TPS I+SA+STTA   D  G
Sbjct: 600 ASTDSTEFEGEKFAMMSGTSMAAPHVAGVAALIKQSYPQFTPSTISSALSTTALLNDNKG 659

Query: 125 DLIQAEGFNLH---ALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVDP 184
             I A+    +   +LY +TP D G+G V+ + ALDPGLVF T +ED I+FLC + G D 
Sbjct: 660 SPIMAQRTYSNPDQSLYTATPSDMGSGFVNATAALDPGLVFDTSFEDYISFLCGINGSD- 719

Query: 185 AVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIPP 244
            VV + TG +C A+ +     DLNLPSIT+S+L G Q  QR ++N+ G  ETY     PP
Sbjct: 720 TVVFNYTGFRCPANNTPVSGFDLNLPSITVSTLSGTQTFQRSMRNIAGN-ETYNVGWSPP 779

Query: 245 NGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVVS 304
            G ++ ++P  F++A  E Q L + +  T  +   +FG I L G+  HI  IP++++   
Sbjct: 780 YGVSMKVSPTQFSIAMGENQVLSVTLTVTKNSSSSSFGRIGLFGNTGHIVNIPVTVIAKI 839

[Close]
BLAST of CSPI01G34240 vs. TAIR 10
Match: AT4G30020.1 (PA-domain containing subtilase family protein )

HSP 1 Score: 242.7 bits (618), Expect = 3.9e-64
Identity = 130/297 (43.77%), Postives = 178/297 (59.93%), Query Frame = 0 

Query: 2   VREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAA 61
           V++FK +  IG+G        AP V+ FS+RGP+  + +   AD+LKPDILAPG  IW+A
Sbjct: 515 VKDFKAEGSIGDGLEPILHKSAPEVALFSARGPNTKDFSFQDADLLKPDILAPGSLIWSA 574

Query: 62  WSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYD 121
           WS     E    G  FAL+SGTSMAAPHI GIAAL+KQK+P W+P+ I SA+ TT+T  D
Sbjct: 575 WSANGTDEANYIGEGFALISGTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTSTVID 634

Query: 122 MNGDLIQAEGFN---LHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPG 181
             G  +QA+ ++      L  +TPFD+G+G V+PS ALDPGL+F   YED I FLC+ PG
Sbjct: 635 RAGRPLQAQQYSETETVTLVKATPFDYGSGHVNPSAALDPGLIFDAGYEDYIGFLCTTPG 694

Query: 182 VDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSV 241
           +D   +K+ T   CN  +   HP++ N PSI IS LV  Q V RRV NV  + ETY  + 
Sbjct: 695 IDAHEIKNFTNTPCNFKM--VHPSNFNTPSIAISHLVRTQTVTRRVTNVAEEEETYTITS 754

Query: 242 IPPNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPL 296
                  + ++PP  TV A   +   + +     T  ++FG++ L GS  H   +P+
Sbjct: 755 RMEPAIAIEVSPPAMTVRAGASRTFSVTLTVRSVTGAYSFGQVTLKGSRGHKVTLPV 809

[Close]
BLAST of CSPI01G34240 vs. TAIR 10
Match: AT4G20430.1 (Subtilase family protein )

HSP 1 Score: 241.5 bits (615), Expect = 8.8e-64
Identity = 135/305 (44.26%), Postives = 193/305 (63.28%), Query Frame = 0 

Query: 5   FKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAAWSP 64
           F   A I  G+ A+F N+AP +  +S+RGPD  +   + AD+LKP+++APG+ IW AWS 
Sbjct: 555 FGAVAAIAGGQNANFSNRAPKIMYYSARGPDPQDSLFNDADILKPNLVAPGNSIWGAWSS 614

Query: 65  LSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYDMNG 124
            +      +G  FA+MSGTSMAAPH+ G+AAL+KQK+  ++PS IASA+STT+  +D  G
Sbjct: 615 AATESTEFEGESFAMMSGTSMAAPHVAGVAALVKQKFRKFSPSAIASALSTTSVLFDNKG 674

Query: 125 DLIQAEGFNLH---ALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVDP 184
           + I A+    +    + P+TPFD G G V+ + ALDPGL+F T +ED ++FLC + G  P
Sbjct: 675 EAIMAQRAYANPDQTISPATPFDMGNGFVNATAALDPGLIFDTSFEDYMSFLCGINGSAP 734

Query: 185 AVVKSATGGQC---NASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSV 244
            VV + TG  C   NA+IS S   DLNLPSIT+S L   + VQR + N+ G  ETY  S+
Sbjct: 735 -VVFNYTGTNCLRNNATISGS---DLNLPSITVSKLNNTRTVQRLMTNIAGN-ETYTVSL 794

Query: 245 IPPNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSIL 304
           I P    +N++P  F++A+ E + L + + A   +   +FG I L G+  HI RIP+S+ 
Sbjct: 795 ITPFDVLINVSPTQFSIASGETKLLSVILTAKRNSSISSFGGIKLLGNAGHIVRIPVSVT 854

The following BLAST results are available for this feature:
BLAST of CSPI01G34240 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F4HYR61.2e-9458.25Subtilisin-like protease SBT2.4 OS=Arabidopsis thaliana OX=3702 GN=SBT2.4 PE=1 S... [more]
O644812.0e-6543.67Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 S... [more]
Q9FI121.9e-6345.36Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana OX=3702 GN=SBT2.3 PE=2 S... [more]
Q9SZV55.5e-6343.77Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 S... [more]
Q9SUN61.2e-6244.26Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana OX=3702 GN=SBT2.2 PE=3 S... [more]
BLAST of CSPI01G34240 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LYA01.1e-170100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G701260 PE=3 SV=1[more]
A0A1S3C9G96.4e-16396.36LOW QUALITY PROTEIN: subtilisin-like protease SBT2.4 OS=Cucumis melo OX=3656 GN=... [more]
A0A5A7TIJ48.4e-15592.72Subtilisin-like protease SBT2.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A5D3CPQ52.7e-15392.38Subtilisin-like protease SBT2.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A6J1CM391.1e-14685.15subtilisin-like protease SBT2.4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC... [more]
BLAST of CSPI01G34240 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004145469.32.2e-170100.00subtilisin-like protease SBT2.4 [Cucumis sativus][more]
KAE8653651.12.2e-170100.00hypothetical protein Csa_006839 [Cucumis sativus][more]
XP_008459133.11.3e-16296.36PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease SBT2.4, partial [Cucumi... [more]
XP_038895834.11.3e-15489.77subtilisin-like protease SBT2.4 [Benincasa hispida][more]
KAA0043154.11.7e-15492.72subtilisin-like protease SBT2.4 [Cucumis melo var. makuwa][more]
BLAST of CSPI01G34240 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G62340.18.7e-9658.25PA-domain containing subtilase family protein [more]
AT2G19170.11.4e-6643.67subtilisin-like serine protease 3 [more]
AT5G44530.11.4e-6445.36Subtilase family protein [more]
AT4G30020.13.9e-6443.77PA-domain containing subtilase family protein [more]
AT4G20430.18.8e-6444.26Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 20..168
e-value: 1.8E-46
score: 160.9
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 22..160
NoneNo IPR availableGENE3D2.60.40.2310coord: 173..300
e-value: 3.6E-16
score: 61.1
NoneNo IPR availablePANTHERPTHR10795:SF384SUBTILISIN-LIKE PROTEASE SBT2.6coord: 12..299
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 1..156
score: 14.356882
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 23..121
e-value: 1.2E-19
score: 70.7
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 203..297
e-value: 2.9E-14
score: 53.1
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 12..299
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 82..92

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G34240.1CSPI01G34240.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090558 plant epidermis development
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity