CSPI01G33370 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G33370
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionBeta_elim_lyase domain-containing protein
LocationChr1: 28278467 .. 28282236 (-)
RNA-Seq ExpressionCSPI01G33370
SyntenyCSPI01G33370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTTTATTATGCAAATTTTGTGTTGAAATGGTGTGTTTAATATATAATAGGCAAGAAGGGTTAGAAAAACATTGGGTGGAGGAATGAGGCAAATTGGGATCCTTTGTGCAGCTGGACTTGTTGCTATCAAAGAGAATGTTCAAAAACTTGAAGCTGATCATAAGAAGGCTAAGCAACTAGCTAGTAATTAACATCACTCTTTCACTATAATTCTTATCAACATTACCAATTACTATACTGATCCTCTTTTTTTAATATTTTTCTTGGGATTAATATAGGTGGGTTATTCCAAATCAAAGGATTAAAGATTGATCCAAAATCAGTTGAGACAAACATTGTAAGTATCTCTAAACAATAATTGAAAATTGTAGTGATTTTTATCATCTTTGTGTTTGAATTGATTTTTTTGTTTCTTTTGATTAAAAGGGTTTAAAAATAGATTTTAAAACTTATGTTTGATTTTAATGTCAATATTTTGTACCGTTAATATGAACCGAAATTATATAGATAAAAAATTATTCGCTCTCTCGATTCTTAAGGAGTGCGTTCAAAACTCTTGAGATGCATTCAATATAGACATACTCGAATGCGTGAGAATCTCAAGATGTTATATTATCTTACATTACGACAATACAAAAACAGTAAACGAATACTTACTCAACGAGAATTTAGTGATATTCATCGAACAATCTTCCTCTAATAGATTACAAAATGAACTATTTACTTAATTGTCTAGACCAAAACTTCAAAATTTATCAATGTTAGGTCCAAAACTTTCAAAAGTGTTAAATGAATCTAAAAATTTAGGTTGGGCTTCGTAGAGCATGAACCGTGTTGATATTGTATCTAATAACTTCTTAAAAAATTTAAAATTCTTCTAAATTAATTCAATCTCTTTTAGATAAAAAAATTAAATTTTGTGGTTAAAGCAGGAGAACACTCAATTTTGTTTTCAGAAATGTTTTGATTATAAAATATTAAAACTCATACATAAATGATACTCATAAATATAACAATTAGATTCAAAATATTAACATATATGATAATATTTTAAAAAATTTGCAAATGTAACAAAATATGTCAAAAAGTCTATTAATGATTGAAGTTTATCATCTATAGAAGTCTACACTGACAGGCTTTGCAATATTTGCATTATTTTAAAAATATTGTTGTACACTTAATTATTGTTCTTAAAATTGCTACACAAATTATAATTATCCTATAAACAAATGTGTAAGTTTTAGTACAAATCTTAACGTCTAGAAAAAAACGAATTGTAAACTATAGTAAATAAAATTACATATAGTTAGATCAATGTTAATTTTCATAAATATAACAAAACATCAAAATATTTACGGTCTATGTAAAAAAAATAAAAAAAACCTTGAAACCGACTTTTTAAAAAAATATTCCATATTTGTCTTCTTTTCCATATTTTTTCTTCTCTTCTCCTACAATTTTTCTTTTCCTCTCTATCATTTTTCTTCTCTTCTTCCCCAATTTTTGTTTTATATTTTTTTAAAATCATGATATTTGTTTTCAATAGATCATATATATATGGTATTTTTTTAAAGTGTTATTTGATTCAAGATCGTATACAAAATATAAAAGATCTTGAAAAAATTCGTTGAAATATTGGTACACGATCATTTAGATTTTCGTGTAACAAAGAATTTTAAAAAAATATTGTTTCAATTTGGCTATCTAAATCAAAACGATAGTTTTCCAAAGGATTTAGAAAAAAAAAATCGTTTAAATTTAGATACTCAAAACTATTTAAATCTAAAGGATCATGTAGTCAAGATTTAATGACGTGTTGTGAGACATTTTTATTATGAGTCCATTGGCATTTTTTCGTTTTTAAAATTGTTTTACGGGGTGGAGTGTAAATATTGAAGCAGATTGTTATAATTTTTAGAAAACGGTTGAGATCAATGTAGGACATAATTTCCATTGACTAATTATTTATTGAAAGCTATTCATATATGTTTGTGAGTTTTATTTTAATACTTAAATTACAAAATTAAAAAAAAATATTTCTTTATTATTATTATTATTATTTTGAAATTATTTATAGTTATTTAGTTTGTTATATATTATCAGATATTGTTTGAAATAGAAGACGATTACGGAATCTCAATGGAAACATTATGTAAAAGCTTGGAAGAACGTGGCATTTTTGTGATGCTACAAACCCAAACAAGGTCTATTCTCAAATTAAAATTCTACTTTTCATTATATATTATTCTTTTTCGTCAAATGTTTGATTAGGAAACATTTTTAATATTTTTTTAAACCATAAAGGTAGAACAAAATCTCACTTATTTCTTATAGCATATATTTTTCACTTGCACGTTTATACGCTAAAATCTCATTTTTCTCGATTCTTAGAGGTGTTATTCTTTGAAAAGTATGAAAGAACATTTTATCTTAGAGCTATTTTTAAAAATACTATTTATGTGTTTTTACTAATTACAATTTATATTAAAAAAATATGAACAAATATATACCATATATAAGATCCTAAAAAAAAATCAATTAACTTTATGAATTGTTTTTAAATATAACAACATGAATCAAAATATTTATAAATATAACAAAATATTATAGTCTTTTTCTAATTTGTTATGCTTTTTTACATTTATAATAGCTTCAACAATTATGTTAATATATTTTTTATTTTTTCGTTAATTTAAAATTTTAGAGAAAAAACCAAACAAAATTGCCAAAATAACACATTTTATATTTTTCTCCATTTTAAAACGAAGTGTGCATGAAAACAAAAGTTTTAGCTAAGAAATGGCAGATGTTTATCCAAAACAATTCAATCTGGCTTCAACAACGAGTGAGTTTCAATAACTTTTGAAATAGTGAAATATTTTAATTAATAGGAAAAATATTTTCAAAAATCTTTTAAAAATGATTGTAACATCCGAAAAGAGAAAAAAAATACGTGTAATAAGATATCACAATCTATTTACGATGAACGGTGTGGTAGATCAAGATAGACTATTATCTCTATTTATGGAGCAATAGTATGAAAACAATAAATTCAATAGGTTGTTGTATTTAGTTATTTATTAAACAAAGTTGAATTGCAGGGCTAGAATTGTTTTTCATCATCAAATTTCAACGAGTGATGTGCAGTATATTTTATCTTGCTTTCAGGTGATCTTCCAATTAGGTCTATTTTTCTTTTTCAATAAAAATATATACGAATTATTTTAGATATATCAAAATGAATCAAAGTATTTATAAAATATAATAAAATTTTAGATTTTATCAGTGACAGATGTCGATAAACTTCTATCAATTTATTTTATTATCTATCAATCATATTGATAGAAATTCATCGGTGATAAAATCTAAAATTTGGTTATATTTATAGAGTAATTTTTCAAAACTATATTATTGTTACAAATATATAAATGTAACATATGAGAAAGAGTTGAAAGTTTATTAACTTTTTTGAAGTTTATATAAGAACTAGCAATAAGTTTTCACATTCTCCACCAAATTGTTGATTAAACTAACATAAACCTTTTTCCCTTTGGCTTTTGCAGCAAACTCTAAATGGAATTAAAGTTGTAAATGGCAACTAATTGATCTCATAAGTAACTGTTTCTCTCTCTCCCTAATAAACAAAGTGGAAGTTACCCACTTTGGCATATATGCTTAATACGCCATTTTCTTTGTTGTATAAAACATTTCTAATCTCTATATTGGGCTATAGTATATCATATTCTCCATTTCTCTTTTCTTGAGTGAGG

mRNA sequence

CCTTTATTATGCAAATTTTGTGTTGAAATGGTGTGTTTAATATATAATAGGCAAGAAGGGTTAGAAAAACATTGGGTGGAGGAATGAGGCAAATTGGGATCCTTTGTGCAGCTGGACTTGTTGCTATCAAAGAGAATGTTCAAAAACTTGAAGCTGATCATAAGAAGGCTAAGCAACTAGCTAGTGGGTTATTCCAAATCAAAGGATTAAAGATTGATCCAAAATCAGTTGAGACAAACATTATATTGTTTGAAATAGAAGACGATTACGGAATCTCAATGGAAACATTATGTAAAAGCTTGGAAGAACGTGGCATTTTTGTGATGCTACAAACCCAAACAAGGGCTAGAATTGTTTTTCATCATCAAATTTCAACGAGTGATGTGCAGTATATTTTATCTTGCTTTCAGCAAACTCTAAATGGAATTAAAGTTGTAAATGGCAACTAATTGATCTCATAAGTAACTGTTTCTCTCTCTCCCTAATAAACAAAGTGGAAGTTACCCACTTTGGCATATATGCTTAATACGCCATTTTCTTTGTTGTATAAAACATTTCTAATCTCTATATTGGGCTATAGTATATCATATTCTCCATTTCTCTTTTCTTGAGTGAGG

Coding sequence (CDS)

ATGAGGCAAATTGGGATCCTTTGTGCAGCTGGACTTGTTGCTATCAAAGAGAATGTTCAAAAACTTGAAGCTGATCATAAGAAGGCTAAGCAACTAGCTAGTGGGTTATTCCAAATCAAAGGATTAAAGATTGATCCAAAATCAGTTGAGACAAACATTATATTGTTTGAAATAGAAGACGATTACGGAATCTCAATGGAAACATTATGTAAAAGCTTGGAAGAACGTGGCATTTTTGTGATGCTACAAACCCAAACAAGGGCTAGAATTGTTTTTCATCATCAAATTTCAACGAGTGATGTGCAGTATATTTTATCTTGCTTTCAGCAAACTCTAAATGGAATTAAAGTTGTAAATGGCAACTAA

Protein sequence

MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIEDDYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTLNGIKVVNGN*
Homology
BLAST of CSPI01G33370 vs. ExPASy Swiss-Prot
Match: Q8RXU4 (Probable low-specificity L-threonine aldolase 1 OS=Arabidopsis thaliana OX=3702 GN=THA1 PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 2.1e-29
Identity = 63/112 (56.25%), Postives = 85/112 (75.89%), Query Frame = 0

Query: 1   MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED 60
           MRQIG+LCAA LVA++EN+ KL+ DHKKAK LA GL Q+KG++++  +VETN+I  ++ED
Sbjct: 238 MRQIGVLCAAALVALQENLPKLQHDHKKAKLLAEGLNQMKGIRVNVAAVETNMIFMDMED 297

Query: 61  DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTL 113
              ++ E L K+LEE GI ++    +R RIV HHQI+TSDV Y LSCFQQ +
Sbjct: 298 GSRLTAEKLRKNLEENGILLIRGNSSRIRIVIHHQITTSDVHYTLSCFQQAM 349

BLAST of CSPI01G33370 vs. ExPASy Swiss-Prot
Match: Q9FPH3 (Probable low-specificity L-threonine aldolase 2 OS=Arabidopsis thaliana OX=3702 GN=THA2 PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 2.6e-27
Identity = 62/110 (56.36%), Postives = 80/110 (72.73%), Query Frame = 0

Query: 1   MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED 60
           MRQIG+LCAA LVA+ ENV KLE DHKKA+ LA GL +I+ L+++  +VETNII  +I +
Sbjct: 242 MRQIGVLCAAALVALHENVAKLEDDHKKARVLAEGLNRIERLRVNVAAVETNIIYVDIPE 301

Query: 61  DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQ 111
           D     E  CKSLE+ G+ V+ Q   R RIV HHQIS  DV+Y+LSCF++
Sbjct: 302 DPKFGAEEACKSLEDVGVLVIPQATFRIRIVLHHQISDVDVEYVLSCFEK 351

BLAST of CSPI01G33370 vs. ExPASy Swiss-Prot
Match: O07051 (L-allo-threonine aldolase OS=Aeromonas jandaei OX=650 GN=ltaA PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 9.8e-11
Identity = 37/112 (33.04%), Postives = 63/112 (56.25%), Query Frame = 0

Query: 1   MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED 60
           MRQ GIL  AGL A++++V +L  DH++A+QLA GL  + G+++D   V+TN++  ++  
Sbjct: 230 MRQAGILAQAGLFALQQHVVRLADDHRRARQLAEGLAALPGIRLDLAQVQTNMVFLQLTS 289

Query: 61  DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTL 113
                +    K+   RGI  +       R+V H QI   D++ ++  F + L
Sbjct: 290 GESAPLLAFMKA---RGI--LFSGYGELRLVTHLQIHDDDIEEVIDAFTEYL 336

BLAST of CSPI01G33370 vs. ExPASy TrEMBL
Match: A0A0A0M3F6 (Beta_elim_lyase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G681120 PE=4 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.5e-59
Identity = 121/121 (100.00%), Postives = 121/121 (100.00%), Query Frame = 0

Query: 1   MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED 60
           MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED
Sbjct: 1   MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED 60

Query: 61  DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTLNGIKVVNG 120
           DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTLNGIKVVNG
Sbjct: 61  DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTLNGIKVVNG 120

Query: 121 N 122
           N
Sbjct: 121 N 121

BLAST of CSPI01G33370 vs. ExPASy TrEMBL
Match: A0A1S3BWA2 (probable low-specificity L-threonine aldolase 1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494316 PE=3 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 6.7e-55
Identity = 110/121 (90.91%), Postives = 118/121 (97.52%), Query Frame = 0

Query: 1   MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED 60
           MRQIGILCAAGLVAIKENVQKLEADH+KAKQLASGL+QIKGLK+DPKSVETNII FEIED
Sbjct: 272 MRQIGILCAAGLVAIKENVQKLEADHEKAKQLASGLYQIKGLKVDPKSVETNIIFFEIED 331

Query: 61  DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTLNGIKVVNG 120
           DYGISMETLCK+LEERGIF+ML++QTRARIV HHQISTSDVQY LSCF+QTLNGIKVVNG
Sbjct: 332 DYGISMETLCKTLEERGIFMMLESQTRARIVLHHQISTSDVQYTLSCFKQTLNGIKVVNG 391

Query: 121 N 122
           N
Sbjct: 392 N 392

BLAST of CSPI01G33370 vs. ExPASy TrEMBL
Match: A0A1S3BXZ8 (probable low-specificity L-threonine aldolase 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494316 PE=3 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 6.7e-55
Identity = 110/121 (90.91%), Postives = 118/121 (97.52%), Query Frame = 0

Query: 1   MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED 60
           MRQIGILCAAGLVAIKENVQKLEADH+KAKQLASGL+QIKGLK+DPKSVETNII FEIED
Sbjct: 280 MRQIGILCAAGLVAIKENVQKLEADHEKAKQLASGLYQIKGLKVDPKSVETNIIFFEIED 339

Query: 61  DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTLNGIKVVNG 120
           DYGISMETLCK+LEERGIF+ML++QTRARIV HHQISTSDVQY LSCF+QTLNGIKVVNG
Sbjct: 340 DYGISMETLCKTLEERGIFMMLESQTRARIVLHHQISTSDVQYTLSCFKQTLNGIKVVNG 399

Query: 121 N 122
           N
Sbjct: 400 N 400

BLAST of CSPI01G33370 vs. ExPASy TrEMBL
Match: A0A6J1DWI0 (probable low-specificity L-threonine aldolase 1 OS=Momordica charantia OX=3673 GN=LOC111024142 PE=3 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 5.9e-43
Identity = 94/122 (77.05%), Postives = 105/122 (86.07%), Query Frame = 0

Query: 1   MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED 60
           MRQIGILC+A LVAIKEN+ KLE DH KAK LASGL +I GLK+DPKSVETNII FE+ED
Sbjct: 238 MRQIGILCSAALVAIKENLPKLEDDHHKAKLLASGLSEINGLKVDPKSVETNIIFFELED 297

Query: 61  -DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTLNGIKVVN 120
            DY IS+ETLCKSLEERGIF+M ++ TRARIV HHQIS SDV Y LSCFQQTL+GI+V N
Sbjct: 298 VDYKISVETLCKSLEERGIFMMQESSTRARIVIHHQISISDVHYTLSCFQQTLSGIQVGN 357

Query: 121 GN 122
           GN
Sbjct: 358 GN 359

BLAST of CSPI01G33370 vs. ExPASy TrEMBL
Match: A0A6J1EZ04 (probable low-specificity L-threonine aldolase 1 OS=Cucurbita moschata OX=3662 GN=LOC111440844 PE=3 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 5.0e-42
Identity = 90/121 (74.38%), Postives = 102/121 (84.30%), Query Frame = 0

Query: 1   MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED 60
           MRQIGILCAA L+AIKENV KL  DH  AK LASGL QI G+K+DPKSVETNII FE+E+
Sbjct: 238 MRQIGILCAAALIAIKENVPKLATDHHNAKLLASGLNQINGVKVDPKSVETNIIFFEMEE 297

Query: 61  DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTLNGIKVVNG 120
           D  IS+ETLCKSLEERGIF+ML  +TRAR+V HHQISTSDV+Y LSCFQQTL+GI   +G
Sbjct: 298 DSKISVETLCKSLEERGIFMMLDGKTRARMVLHHQISTSDVEYTLSCFQQTLSGIAAADG 357

Query: 121 N 122
           N
Sbjct: 358 N 358

BLAST of CSPI01G33370 vs. NCBI nr
Match: XP_011660269.1 (probable low-specificity L-threonine aldolase 1 isoform X2 [Cucumis sativus] >XP_031745916.1 probable low-specificity L-threonine aldolase 1 isoform X2 [Cucumis sativus] >KAE8637487.1 hypothetical protein CSA_016969 [Cucumis sativus] >KAE8653610.1 hypothetical protein Csa_006916 [Cucumis sativus])

HSP 1 Score: 238.4 bits (607), Expect = 3.2e-59
Identity = 121/121 (100.00%), Postives = 121/121 (100.00%), Query Frame = 0

Query: 1   MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED 60
           MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED
Sbjct: 238 MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED 297

Query: 61  DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTLNGIKVVNG 120
           DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTLNGIKVVNG
Sbjct: 298 DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTLNGIKVVNG 357

Query: 121 N 122
           N
Sbjct: 358 N 358

BLAST of CSPI01G33370 vs. NCBI nr
Match: XP_031736719.1 (probable low-specificity L-threonine aldolase 1 isoform X1 [Cucumis sativus] >XP_031736720.1 probable low-specificity L-threonine aldolase 1 isoform X1 [Cucumis sativus] >XP_031736728.1 probable low-specificity L-threonine aldolase 1 isoform X1 [Cucumis sativus] >XP_031745913.1 probable low-specificity L-threonine aldolase 1 isoform X1 [Cucumis sativus] >XP_031745914.1 probable low-specificity L-threonine aldolase 1 isoform X1 [Cucumis sativus] >XP_031745915.1 probable low-specificity L-threonine aldolase 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 231.9 bits (590), Expect = 3.0e-57
Identity = 121/127 (95.28%), Postives = 121/127 (95.28%), Query Frame = 0

Query: 1   MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNI------I 60
           MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNI      I
Sbjct: 238 MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIFVIYYQI 297

Query: 61  LFEIEDDYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTLNG 120
           LFEIEDDYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTLNG
Sbjct: 298 LFEIEDDYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTLNG 357

Query: 121 IKVVNGN 122
           IKVVNGN
Sbjct: 358 IKVVNGN 364

BLAST of CSPI01G33370 vs. NCBI nr
Match: XP_008453667.1 (PREDICTED: probable low-specificity L-threonine aldolase 1 isoform X2 [Cucumis melo])

HSP 1 Score: 223.0 bits (567), Expect = 1.4e-54
Identity = 110/121 (90.91%), Postives = 118/121 (97.52%), Query Frame = 0

Query: 1   MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED 60
           MRQIGILCAAGLVAIKENVQKLEADH+KAKQLASGL+QIKGLK+DPKSVETNII FEIED
Sbjct: 272 MRQIGILCAAGLVAIKENVQKLEADHEKAKQLASGLYQIKGLKVDPKSVETNIIFFEIED 331

Query: 61  DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTLNGIKVVNG 120
           DYGISMETLCK+LEERGIF+ML++QTRARIV HHQISTSDVQY LSCF+QTLNGIKVVNG
Sbjct: 332 DYGISMETLCKTLEERGIFMMLESQTRARIVLHHQISTSDVQYTLSCFKQTLNGIKVVNG 391

Query: 121 N 122
           N
Sbjct: 392 N 392

BLAST of CSPI01G33370 vs. NCBI nr
Match: XP_008453666.1 (PREDICTED: probable low-specificity L-threonine aldolase 1 isoform X1 [Cucumis melo])

HSP 1 Score: 223.0 bits (567), Expect = 1.4e-54
Identity = 110/121 (90.91%), Postives = 118/121 (97.52%), Query Frame = 0

Query: 1   MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED 60
           MRQIGILCAAGLVAIKENVQKLEADH+KAKQLASGL+QIKGLK+DPKSVETNII FEIED
Sbjct: 280 MRQIGILCAAGLVAIKENVQKLEADHEKAKQLASGLYQIKGLKVDPKSVETNIIFFEIED 339

Query: 61  DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTLNGIKVVNG 120
           DYGISMETLCK+LEERGIF+ML++QTRARIV HHQISTSDVQY LSCF+QTLNGIKVVNG
Sbjct: 340 DYGISMETLCKTLEERGIFMMLESQTRARIVLHHQISTSDVQYTLSCFKQTLNGIKVVNG 399

Query: 121 N 122
           N
Sbjct: 400 N 400

BLAST of CSPI01G33370 vs. NCBI nr
Match: XP_038877077.1 (probable low-specificity L-threonine aldolase 1 [Benincasa hispida])

HSP 1 Score: 216.5 bits (550), Expect = 1.3e-52
Identity = 109/121 (90.08%), Postives = 113/121 (93.39%), Query Frame = 0

Query: 1   MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED 60
           MRQIGILCAAGLVA+KENV KLEADH KAKQLASGL QIKGLKIDPKSVETNII FEIED
Sbjct: 238 MRQIGILCAAGLVALKENVHKLEADHDKAKQLASGLHQIKGLKIDPKSVETNIIFFEIED 297

Query: 61  DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTLNGIKVVNG 120
           DYGISMETLCKSLEERGIF+ML++Q RARIV HHQISTSDVQY LSCFQQTLNGIKV NG
Sbjct: 298 DYGISMETLCKSLEERGIFMMLESQIRARIVLHHQISTSDVQYTLSCFQQTLNGIKVENG 357

Query: 121 N 122
           N
Sbjct: 358 N 358

BLAST of CSPI01G33370 vs. TAIR 10
Match: AT1G08630.1 (threonine aldolase 1 )

HSP 1 Score: 129.8 bits (325), Expect = 1.5e-30
Identity = 63/112 (56.25%), Postives = 85/112 (75.89%), Query Frame = 0

Query: 1   MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED 60
           MRQIG+LCAA LVA++EN+ KL+ DHKKAK LA GL Q+KG++++  +VETN+I  ++ED
Sbjct: 238 MRQIGVLCAAALVALQENLPKLQHDHKKAKLLAEGLNQMKGIRVNVAAVETNMIFMDMED 297

Query: 61  DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTL 113
              ++ E L K+LEE GI ++    +R RIV HHQI+TSDV Y LSCFQQ +
Sbjct: 298 GSRLTAEKLRKNLEENGILLIRGNSSRIRIVIHHQITTSDVHYTLSCFQQAM 349

BLAST of CSPI01G33370 vs. TAIR 10
Match: AT1G08630.3 (threonine aldolase 1 )

HSP 1 Score: 129.8 bits (325), Expect = 1.5e-30
Identity = 63/112 (56.25%), Postives = 85/112 (75.89%), Query Frame = 0

Query: 1   MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED 60
           MRQIG+LCAA LVA++EN+ KL+ DHKKAK LA GL Q+KG++++  +VETN+I  ++ED
Sbjct: 238 MRQIGVLCAAALVALQENLPKLQHDHKKAKLLAEGLNQMKGIRVNVAAVETNMIFMDMED 297

Query: 61  DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTL 113
              ++ E L K+LEE GI ++    +R RIV HHQI+TSDV Y LSCFQQ +
Sbjct: 298 GSRLTAEKLRKNLEENGILLIRGNSSRIRIVIHHQITTSDVHYTLSCFQQAM 349

BLAST of CSPI01G33370 vs. TAIR 10
Match: AT1G08630.2 (threonine aldolase 1 )

HSP 1 Score: 129.8 bits (325), Expect = 1.5e-30
Identity = 63/112 (56.25%), Postives = 85/112 (75.89%), Query Frame = 0

Query: 1   MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED 60
           MRQIG+LCAA LVA++EN+ KL+ DHKKAK LA GL Q+KG++++  +VETN+I  ++ED
Sbjct: 238 MRQIGVLCAAALVALQENLPKLQHDHKKAKLLAEGLNQMKGIRVNVAAVETNMIFMDMED 297

Query: 61  DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTL 113
              ++ E L K+LEE GI ++    +R RIV HHQI+TSDV Y LSCFQQ +
Sbjct: 298 GSRLTAEKLRKNLEENGILLIRGNSSRIRIVIHHQITTSDVHYTLSCFQQAM 349

BLAST of CSPI01G33370 vs. TAIR 10
Match: AT1G08630.4 (threonine aldolase 1 )

HSP 1 Score: 129.8 bits (325), Expect = 1.5e-30
Identity = 63/112 (56.25%), Postives = 85/112 (75.89%), Query Frame = 0

Query: 1   MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED 60
           MRQIG+LCAA LVA++EN+ KL+ DHKKAK LA GL Q+KG++++  +VETN+I  ++ED
Sbjct: 238 MRQIGVLCAAALVALQENLPKLQHDHKKAKLLAEGLNQMKGIRVNVAAVETNMIFMDMED 297

Query: 61  DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQTL 113
              ++ E L K+LEE GI ++    +R RIV HHQI+TSDV Y LSCFQQ +
Sbjct: 298 GSRLTAEKLRKNLEENGILLIRGNSSRIRIVIHHQITTSDVHYTLSCFQQAM 349

BLAST of CSPI01G33370 vs. TAIR 10
Match: AT3G04520.1 (threonine aldolase 2 )

HSP 1 Score: 122.9 bits (307), Expect = 1.8e-28
Identity = 62/110 (56.36%), Postives = 80/110 (72.73%), Query Frame = 0

Query: 1   MRQIGILCAAGLVAIKENVQKLEADHKKAKQLASGLFQIKGLKIDPKSVETNIILFEIED 60
           MRQIG+LCAA LVA+ ENV KLE DHKKA+ LA GL +I+ L+++  +VETNII  +I +
Sbjct: 242 MRQIGVLCAAALVALHENVAKLEDDHKKARVLAEGLNRIERLRVNVAAVETNIIYVDIPE 301

Query: 61  DYGISMETLCKSLEERGIFVMLQTQTRARIVFHHQISTSDVQYILSCFQQ 111
           D     E  CKSLE+ G+ V+ Q   R RIV HHQIS  DV+Y+LSCF++
Sbjct: 302 DPKFGAEEACKSLEDVGVLVIPQATFRIRIVLHHQISDVDVEYVLSCFEK 351

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RXU42.1e-2956.25Probable low-specificity L-threonine aldolase 1 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9FPH32.6e-2756.36Probable low-specificity L-threonine aldolase 2 OS=Arabidopsis thaliana OX=3702 ... [more]
O070519.8e-1133.04L-allo-threonine aldolase OS=Aeromonas jandaei OX=650 GN=ltaA PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0M3F61.5e-59100.00Beta_elim_lyase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G68... [more]
A0A1S3BWA26.7e-5590.91probable low-specificity L-threonine aldolase 1 isoform X2 OS=Cucumis melo OX=36... [more]
A0A1S3BXZ86.7e-5590.91probable low-specificity L-threonine aldolase 1 isoform X1 OS=Cucumis melo OX=36... [more]
A0A6J1DWI05.9e-4377.05probable low-specificity L-threonine aldolase 1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1EZ045.0e-4274.38probable low-specificity L-threonine aldolase 1 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
XP_011660269.13.2e-59100.00probable low-specificity L-threonine aldolase 1 isoform X2 [Cucumis sativus] >XP... [more]
XP_031736719.13.0e-5795.28probable low-specificity L-threonine aldolase 1 isoform X1 [Cucumis sativus] >XP... [more]
XP_008453667.11.4e-5490.91PREDICTED: probable low-specificity L-threonine aldolase 1 isoform X2 [Cucumis m... [more]
XP_008453666.11.4e-5490.91PREDICTED: probable low-specificity L-threonine aldolase 1 isoform X1 [Cucumis m... [more]
XP_038877077.11.3e-5290.08probable low-specificity L-threonine aldolase 1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G08630.11.5e-3056.25threonine aldolase 1 [more]
AT1G08630.31.5e-3056.25threonine aldolase 1 [more]
AT1G08630.21.5e-3056.25threonine aldolase 1 [more]
AT1G08630.41.5e-3056.25threonine aldolase 1 [more]
AT3G04520.11.8e-2856.36threonine aldolase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 12..32
NoneNo IPR availablePANTHERPTHR48097L-THREONINE ALDOLASE-RELATEDcoord: 1..121
NoneNo IPR availablePANTHERPTHR48097:SF4L-ALLO-THREONINE ALDOLASE-LIKE PROTEINcoord: 1..121
IPR001597Aromatic amino acid beta-eliminating lyase/threonine aldolasePFAMPF01212Beta_elim_lyasecoord: 1..55
e-value: 1.5E-7
score: 31.0
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 18..113
e-value: 3.7E-25
score: 90.0
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 1..114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G33370.1CSPI01G33370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006520 cellular amino acid metabolic process
molecular_function GO:0016829 lyase activity
molecular_function GO:0003824 catalytic activity