CSPI01G30700 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G30700
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionphytochromobilin:ferredoxin oxidoreductase, chloroplastic
LocationChr1: 25198728 .. 25204685 (-)
RNA-Seq ExpressionCSPI01G30700
SyntenyCSPI01G30700
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGGCAACGTGGGTCAACCACATAATCCAAAATCCTTGTTCTTCATAGCTCAAACCCCAACACTGGATCGATTTGAGAGATACAAAATGGGATTTCAGTAACACAAATGCTTCAACTGAACCCTTTATTATAAGTTTTACTCCAATGGGTTCTTCTCGTTTGTGCTTCTCCCTTTCCTTCAACTCTCCACCATTTTCAACCAACTGTAGCAGTAAAAACAGGAGTAAAAGAGCTTTCTTTCCCATTTCTTCAATTTCATATCAGAAATTTGTTCAATTCGCTTTGGATGAAACCAAACTTCACACCCATCTCTTCCCCTCCCCTTTGCAGGTAATGGATTAATCTACTTGATATTGTATTTGGGGCCTTCTGATTTTGCTTCACTTGAGCTTCATTAATCTATTTTTCTTTGTAATGGTTTCAAACAAATGTTTGGCTGTTCTTCTCTCTAGATTGGTTTTTGTTTTCTTTGTTTAATAGCGTCTGTTAAGAACAGGGATGCGTTTTGTTGTGTTTCTTGCAACTTCCCCTGTTCTACTGTGAGTTTGTCTGAGCTTTTTTCAGTTTGACATGTTCTTCTCTTTTTTACTGATTCTGTGTGGAGAGAATTGATGGACTTGTTGTTTTAGATTAGTTTTCATTGTATTATCTTTTAATGGACTGGAAATGACTCTCTAGCAAAGTTTTCTGAGTTCTTAACTTATCATGAGAATACACAGAAAATATCGTGTTTTCAATCTTATTATCATTGATATAAATCAACAGTGTCCATTAGACTTACCACATCATAATAATATTAATAACAGGGTCTACCTGAAATGGAGTTGAAACTTTAATATAAATCAAGGAGATTTGTTGGATTGGTTTCTTCTTGCTGGATTATAGCGGCTGTTTCTTTTTTCAGTATAGATTCCACGTCTTTTTATGTTAGGATATCGAAAAACTTGAGCGATTCATCATCATATCTAAACTATTACTTAAGATTATAGATTATTTTTTATCTAGGATGCACAGGTTGTCAACTCGCTAATTAGTGAATACCCTATTTGATGTTCTAATGGATTGTTTGACTTTTTGTCAACTCAAGTCAATATAATGCAATCGTTTGAGGCTTGCATTTACATACATGGTTGTGTTGTATGTATCTTTGCTGTTATTGTCGACATTGTCAGAATGAATAAAGTTTTAAACTCTGTATGAATTTAAACGCAAGTCATTGTTTCTACCATCCTAACAATAGAATTATTCTTATCCTGCAGGAAAAACATAATTCCATGCTTTCGTTGGACCAAAAAGCCAAGCTTGAAATGTTATCTTTCCATGCTCCTAAGATCAGACTACTTCGCAGTTTGAATATCGAAAGTGAAACAGTGCAGGTTTTATTTTCTATTTCAACCCTCTTAGGGATAATTTTTATGCTTTAGTTCATATAATATGATTGGAAAATGAAACTTAAGAGAATTTGAGCTGTACCAGGTAAATTGTAAAGTGATTTGACAGAGAAAAAATTGGAGGAGACTCTAGTGACCACTTCCATCTTTTTCGAGAAGGGAAACAAATTCCATTGATGATTGAAACAAAATGAATAAACCCCAAAACATCTATAGGTGGATTACATGAAAATTTTCTGATTGAAAATCAGATACAAGAGACTATAATCAGAAATGAAAGAAGTTAATTTTGTGGGCAACCGTCAAAGTTATATTACATTGATGAATAAAATAAAATGGAAAATATTGTACTACTTTGACGGTTGCCCACAAAATTACATGATCATCGTTTCTATCTGTTTATGAAACTGTGGAGAAAAATCAAATTTTATAATGTGAGATTAAAGGTATTTCAGTAACTTCAGAAAGGTCATTCTCTCATCACCATTATTGTCATCATAATGCCCACACAAAAAAAAAAAGTATTAATATTCAAACTTATTAGACCACAAGAAATAACTTACCAAACTCTGTTGGGTGTTCAAAGAATAAAAGAAAAAATTCTGTAATTATAGCCTATTAACAGTTATTTTAAATTCTAAAGCTCTTGTTTTGCAGCTTTTAGGCAAGTAATTGTGCAAGACAAGATGTTTGTCACCCCTAGCCTGTGATATTCTTATTTCGTTTATTTCTTATTCATAGTTGTCATTTTTTTTTAATAACAAACAGAAAGTAATTCGTAGTTTTGATTAGTTCTCTTGCCCTTTTTGCTTGTAGCCTTCTTGATTGTTCCTTGATGAAATCTCTGCTTCCCCTCAAATTTAAAATTTGAAAATGTTAAATGAAGAACCATCCCTTGAGAAAGCCTTCCTTGGAACTCTAAACTCTAAAGTACAGAAATTTGATAGTTATCCCAAGATAGTCCTTGTACTTTGTTGATGATGAGAGAGTTAGTCTCAGTCATGGACTAAAAAGTCCTTTGGTTATGGCTATGATGGGAATATGATCCATGTGGCTAATCACTGTAGATACTCGCTGCAAGAATTAGTATCAATGATTTTGAGGTTTCTGAATTACTTGAGTCAGCTCTAAACAAATTTCTAAATTGCAGTTTGTGTAGCTAGCCCAACCGTAAATCTTATTTTTCCCTTTTGCTACTATTTACACTTTCAAAATAGCCCTAAACTGCTGAAATTCACTCCAGAATCTGTGTGTTTGGTAACCATACGTAGCATCTATATTTTATATTCAAATAACTATTTCCCTTCAATTATGGTGCAGGTTTTAGATTTTGCTGCATTCCCTGAACCAGAATTTGATATTCCCATTTTTTGCGCAAATATTTTCACTGCTGCAAGTTCAAGTATAGTAGTACTGTAAGCTTCTTTGTACTTTGGAGCCTGATAGCAGATACTAAATTGTAGAACAAATAGCCTTTCATCATTCATATGAGCTTCAATGTTTCAAAGTCTTTGTAAACTTTTGCATCTTCTTCCATGGAACTGAAATACTAAAATTCGATGTTCTTAATATGAGAACCCCAATGAATCAATCTAAAAATCCATTGGTTATGTGAATGCCTTGCCTCCTGTTAACAATGATATCTGAAAAGTCAATTTAGTTCAAATCAAAGGATGGAGGTAGATGCAGATCATTGACTCTAGATCAAAACCTAGAAGAAAGAACCTGAATCTTGTGGGAAGGGGTCATATCTATTGTAGTTTTCCCAATTTCTCTCAGTTTGTTTGTTGGTTGCTGTGTTAGGGAAGGCTCAAAAGAAATAGGTGGGAGATAAGCCACTGTCTGTTTTATTTCCGTATTTGTACTTTCTTTCGAAGCAGCTCTGCGTATAGTGGCCTATTCCCAAGACCATTCTTTGATTTTATTAGGTTTTAGTCATCCTAAGTTTGATAGTGAGGATTGATATGGCGGGTTTTCTTGGAGGGTTAAAATTCCAAAGAGTGTAAAGAGTTTTTCGTGGGAGGTTTAATACCATTGATTGTATTAAAAAAAAACACCCTTCCTTTGTTTTGTATATGCAAGGGTTTAAGAGGCACTAGGTGGATCTTAATCATTTACTAGTTTGTAGACTACCTTGAAGCTGCTTGTTGAGTTTTTTCATTCTGTGGGGTTCGCAATAGTGATGGGCTGTCTATATTGGAGAAGTTGCTTTTGATCCTCCCTAAGTAGAGGGGCAAAGTTTTGTGGCATGGCAACTTCTTTGATGTCTTGTTTTATCTATGAAATGTTAACTTCTGAATCTTTACTGTTTCGTGGGTTATTTGGCTTTGAGAACAGTAAGATTTTCTGGAAAGGTCGTGAAATCATGTGAGGAGTTTTGGGAGGTGGAAATGTTAAATACTTATTTGTGGATGTCAATCACTTGGCCTTTTTGTAGTTATAGGGCCATACTACCATCTTCTTGGGGCTTGTTTTTTATACGTCGTCATTTTATTCTATCATTCTTCTCAATAAAAGCTTTGTTTCGTTATCAAAAAATGTTTGATGCTCAATCAATCGTTAGTGTTCCTAAAGCATGTCATCTAATCAGTAGATCGTAAACGTACTCCTCTACACTTTTTTCGACATACATTAAGTTACATAGATAAGAGAATGTGGGCATCTTGTCTTCTGATTTAGTGAGCTTTCTCCAGTTTTTGTTGGTAGGCACTTGTTGGAAACTTAAGTTAGAGATAAATGTCAACTAAAATGACCAGAATAGTTTCCATAAGTACTTTTTTAGCAATCTCAATCACATACCTGGAGGGAGAAAGATGTTGATTTGGAGTGATATGAGTTTGGCTCATTGATGCATCTAGAGTTGGGGTTTTAAAGCAGACACCTAATCGTGCACCATATACTTCTTATTCCGAGTGTCCAAATTATATGCTAAAATATCTCTAACGAGATTACCAATTAATGTTCTTTTTCTTGCTGGAACTTAACACTAAGTATAATTTGCATCCATTCATAACACCAACTCGTATTTCTTTTGTAGGGATCTCAACCCTTTGCATGATGTGATCTCCCAAAGATATTATAGAGAAAAGTACTATAAGAGCTTATTACATCTTGGAACTAAATATGCTGAGGTGAATGTCTATTTTCAAGATATTAGTCATATTACTATTTACTAACTTCTACTGGCACTCTAGAATTTTTACTTTTATCACATTTTTCATACAACTTTTTTTCCTTTTCCTAAGTCTTCTAAATTTGATGTGATGATTACTGAAAACAGACTTCCTGCAGCTCTTACCCTGGGGCGGAAAGCTTACAAGCGAGTCATTGAAATTTTTCTCTCCCATTGTGATATGGTCCAAGTTTTCATCAACTGAGTCCAAGTATGATATATTGTTTTCTGCATTCATGGATTACTACAAGGTAGTTGAAATTAACTATGCTTCTACTACGATAAAGATTTTTTTTTCTTTTTTTTTCTTTTTTTGCATTTTCTTGTGTGATTTCTTGCTTTGTATTTTTGAAGTATTTTAGTTATATAGATTGGATCTTATTTTGATTATGTTACTGAAAGTTTCAATTTACTGCTAGGCATGGCTTGAGCTCATGAACAATACTGGGGAGGAAACTATCCCGCCTCAACTTATGGTTAATCGAGAAGCGCAACACAGATACCTGTCGTGGAGGGCAGAAAAGGTCATTAACATTTGTTTCATATCATAGATCTCTTCTTCTTTCCTTATATGTTTTGTTTTATGAAAATTTCTGACTTAGCTCTAGCGCAAAGACGTGCTGGTTGTATATGGATGGATTTTACCAACACTTAGCTAGATAATTGCTCACAGTTAACTTTGGTTATATATTCAATTGTAAACCAGAAAGAACATTTAGGAGTCTACTAAATTGGTTTTCATTATACCACTGAAATAAAACTTAATCCAGACTACAAAGTTTCATGAAAACCTTCTCTTTTGTTAAAATGTCTGGGCCTTTATTCGATGCAACATTGGCAAAACAGGATCCAGGACACCATCTTCTAAGAAAACTAATTGGAGAGACGCTTGCAAAGGTAACACACATGAAACCTGTAAATTTTCTTGCATCGTTTGAAAAATCTTCATTCTCTCACTTTGCATCTAACCGTTAGGATGTGGTGAGAAATTTCCTGTTCCATGGCGTCGACGAGCTTGGAAGCAAAAAGTTTCTAGATTACTTTCCAGAGTATTGCTGCGAAGATGGTACAATCAACACGAAACGCAGTATGGCAGGAAAGTCTTACGAACATCGACCTTGGGATGAAAGAGGTGAATTTATTGGAGATACTATCAAAACCTGATATTAGTTCTCTAAATTGATGTAGGTGTAACTTGTAAATTAGATAAGTTATTGTTTGCAAGCAAGTAGCTGGATGCACTGCCATTGAAGTAATCAATTTTTCAATGAGCCACAACAATTTTTAGTTTGTTCTCTAAGCTCATCCAGTTTTCAGATCCTATGTTTATTTTGTAGCATGTCTTTAGGAACTATCGTACATTTAGATTTGAATATATGTAGTCTACTTTGGTTC

mRNA sequence

TTGGCAACGTGGGTCAACCACATAATCCAAAATCCTTGTTCTTCATAGCTCAAACCCCAACACTGGATCGATTTGAGAGATACAAAATGGGATTTCAGTAACACAAATGCTTCAACTGAACCCTTTATTATAAGTTTTACTCCAATGGGTTCTTCTCGTTTGTGCTTCTCCCTTTCCTTCAACTCTCCACCATTTTCAACCAACTGTAGCAGTAAAAACAGGAGTAAAAGAGCTTTCTTTCCCATTTCTTCAATTTCATATCAGAAATTTGTTCAATTCGCTTTGGATGAAACCAAACTTCACACCCATCTCTTCCCCTCCCCTTTGCAGGAAAAACATAATTCCATGCTTTCGTTGGACCAAAAAGCCAAGCTTGAAATGTTATCTTTCCATGCTCCTAAGATCAGACTACTTCGCAGTTTGAATATCGAAAGTGAAACAGTGCAGGTTTTAGATTTTGCTGCATTCCCTGAACCAGAATTTGATATTCCCATTTTTTGCGCAAATATTTTCACTGCTGCAAGTTCAAGTATAGTAGTACTGGATCTCAACCCTTTGCATGATGTGATCTCCCAAAGATATTATAGAGAAAAGTACTATAAGAGCTTATTACATCTTGGAACTAAATATGCTGAGCTCTTACCCTGGGGCGGAAAGCTTACAAGCGAGTCATTGAAATTTTTCTCTCCCATTGTGATATGGTCCAAGTTTTCATCAACTGAGTCCAAGTATGATATATTGTTTTCTGCATTCATGGATTACTACAAGGCATGGCTTGAGCTCATGAACAATACTGGGGAGGAAACTATCCCGCCTCAACTTATGGTTAATCGAGAAGCGCAACACAGATACCTGTCGTGGAGGGCAGAAAAGGATCCAGGACACCATCTTCTAAGAAAACTAATTGGAGAGACGCTTGCAAAGGATGTGGTGAGAAATTTCCTGTTCCATGGCGTCGACGAGCTTGGAAGCAAAAAGTTTCTAGATTACTTTCCAGAGTATTGCTGCGAAGATGGTACAATCAACACGAAACGCAGTATGGCAGGAAAGTCTTACGAACATCGACCTTGGGATGAAAGAGGTGAATTTATTGGAGATACTATCAAAACCTGATATTAGTTCTCTAAATTGATGTAGGTGTAACTTGTAAATTAGATAAGTTATTGTTTGCAAGCAAGTAGCTGGATGCACTGCCATTGAAGTAATCAATTTTTCAATGAGCCACAACAATTTTTAGTTTGTTCTCTAAGCTCATCCAGTTTTCAGATCCTATGTTTATTTTGTAGCATGTCTTTAGGAACTATCGTACATTTAGATTTGAATATATGTAGTCTACTTTGGTTC

Coding sequence (CDS)

ATGGGTTCTTCTCGTTTGTGCTTCTCCCTTTCCTTCAACTCTCCACCATTTTCAACCAACTGTAGCAGTAAAAACAGGAGTAAAAGAGCTTTCTTTCCCATTTCTTCAATTTCATATCAGAAATTTGTTCAATTCGCTTTGGATGAAACCAAACTTCACACCCATCTCTTCCCCTCCCCTTTGCAGGAAAAACATAATTCCATGCTTTCGTTGGACCAAAAAGCCAAGCTTGAAATGTTATCTTTCCATGCTCCTAAGATCAGACTACTTCGCAGTTTGAATATCGAAAGTGAAACAGTGCAGGTTTTAGATTTTGCTGCATTCCCTGAACCAGAATTTGATATTCCCATTTTTTGCGCAAATATTTTCACTGCTGCAAGTTCAAGTATAGTAGTACTGGATCTCAACCCTTTGCATGATGTGATCTCCCAAAGATATTATAGAGAAAAGTACTATAAGAGCTTATTACATCTTGGAACTAAATATGCTGAGCTCTTACCCTGGGGCGGAAAGCTTACAAGCGAGTCATTGAAATTTTTCTCTCCCATTGTGATATGGTCCAAGTTTTCATCAACTGAGTCCAAGTATGATATATTGTTTTCTGCATTCATGGATTACTACAAGGCATGGCTTGAGCTCATGAACAATACTGGGGAGGAAACTATCCCGCCTCAACTTATGGTTAATCGAGAAGCGCAACACAGATACCTGTCGTGGAGGGCAGAAAAGGATCCAGGACACCATCTTCTAAGAAAACTAATTGGAGAGACGCTTGCAAAGGATGTGGTGAGAAATTTCCTGTTCCATGGCGTCGACGAGCTTGGAAGCAAAAAGTTTCTAGATTACTTTCCAGAGTATTGCTGCGAAGATGGTACAATCAACACGAAACGCAGTATGGCAGGAAAGTCTTACGAACATCGACCTTGGGATGAAAGAGGTGAATTTATTGGAGATACTATCAAAACCTGA

Protein sequence

MGSSRLCFSLSFNSPPFSTNCSSKNRSKRAFFPISSISYQKFVQFALDETKLHTHLFPSPLQEKHNSMLSLDQKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCANIFTAASSSIVVLDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFFSPIVIWSKFSSTESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWRAEKDPGHHLLRKLIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSMAGKSYEHRPWDERGEFIGDTIKT*
Homology
BLAST of CSPI01G30700 vs. ExPASy Swiss-Prot
Match: Q9SR43 (Phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HY2 PE=2 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 1.6e-100
Identity = 171/305 (56.07%), Postives = 232/305 (76.07%), Query Frame = 0

Query: 13  NSPPFSTNCSSKNRSKRAFFPISSISYQKFVQFALDETKLHTHLFPSPLQEKHNSMLSLD 72
           ++ P   N + + R KR    +S++SY++F + AL+ET+    L PS LQEK++SM  LD
Sbjct: 25  SASPNKINFTLRRRKKRFLLRVSAVSYKEFAESALEETRKRIVLEPSHLQEKYSSMTGLD 84

Query: 73  QKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCANIFTAASSSIVV 132
            K +L+ML+F + KIRLLRS+ IE+ET+QV DFA F EPE+D PIFCAN FT+ + +IVV
Sbjct: 85  GKTELQMLAFKSSKIRLLRSMAIENETMQVFDFAGFMEPEYDTPIFCANFFTSTNVNIVV 144

Query: 133 LDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFFSPIVIWSKFSST 192
           LDLNPLH +  Q  Y++KYY  ++ +  KYAE  PWGGKLT ES+KFFSP+V+W++FSS+
Sbjct: 145 LDLNPLHQLTDQTDYQDKYYNKIMSIYHKYAETFPWGGKLTGESIKFFSPLVMWTRFSSS 204

Query: 193 ESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWRAEKDPGHHLLRK 252
           + K+  LFSAF++YY+AWLE+     EE  P  +  N EAQH+YL+WRA+KDPGH LL++
Sbjct: 205 KEKHKALFSAFLEYYQAWLEMTIQVREEMEPSHVRANCEAQHKYLTWRAQKDPGHGLLKR 264

Query: 253 LIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSMAGKSYEHRPWDER 312
           L+GE  AK+++R+FLF+GVDELG+K F+DYFPEY  EDGT++ KRS+ GKSYE RPWD  
Sbjct: 265 LVGEAKAKELLRDFLFNGVDELGTKTFIDYFPEYQTEDGTVSDKRSIIGKSYETRPWDLT 324

Query: 313 GEFIG 318
           G+FIG
Sbjct: 325 GQFIG 329

BLAST of CSPI01G30700 vs. ExPASy Swiss-Prot
Match: Q10UW6 (Phycoerythrobilin:ferredoxin oxidoreductase OS=Trichodesmium erythraeum (strain IMS101) OX=203124 GN=pebB PE=3 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.5e-29
Identity = 73/237 (30.80%), Postives = 122/237 (51.48%), Query Frame = 0

Query: 39  YQKFVQFALDETKLHTHLFPSPLQEKHNSMLSLDQKAKLEM------LSFHAPKIRLLRS 98
           YQ F+ +AL + K    L P P+       +++  K K +        +  +PK+R +R+
Sbjct: 11  YQPFLDYALTKLKEELELQPYPIPSGFEHKVAMTGKGKKQQEVQTTSYAHCSPKLRQIRA 70

Query: 99  LNIE-SETVQVLDFAAFPEPEFDIPIFCANIFTAASSSIVVLDLNPLHDVISQRYYREKY 158
            +++    +QVL+F  FP   +D+P F A++ +     ++ LD+ PL        Y++KY
Sbjct: 71  AHVQGGSALQVLNFVIFPHLNYDLPFFGADLVSLPGGHLIALDMQPLFR--DNLQYQKKY 130

Query: 159 YKSLLHLGTKYAELLPWGGKLTSESLKFFSPIVIWSKFSSTESKYDILFSAFMDYYKAWL 218
            + +     K+ + LPWGG    E+  FFSP  +W++   TE     +F AF DY +A+L
Sbjct: 131 TEPIWETFQKHQKYLPWGGDFPEEATPFFSPAFLWTRPQKTELVETRVFEAFKDYLQAYL 190

Query: 219 ELMNNTGEETIPPQLMVNREAQHRYLSWRAEKDPGHHLLRKLIGETLAKDVVRNFLF 269
             +  T + T   +L   +EAQ RYL +RAEKDP   +L +  G    ++ +  FLF
Sbjct: 191 IFVKQTEQITNSEKLAEIKEAQLRYLRYRAEKDPARKMLTRFYGHEWTEEYIHGFLF 245

BLAST of CSPI01G30700 vs. ExPASy Swiss-Prot
Match: Q93TM8 (Phycoerythrobilin:ferredoxin oxidoreductase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) OX=63737 GN=pebB PE=3 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.6e-26
Identity = 69/237 (29.11%), Postives = 119/237 (50.21%), Query Frame = 0

Query: 39  YQKFVQFALDETKLHTHLFPSPLQEKHNSMLSLDQKAKLE------MLSFHAPKIRLLRS 98
           YQ F+ +A+   +    L P P+     S  ++  K K +        +F   K+R +R+
Sbjct: 11  YQPFLDYAIAYMRSRLDLEPYPIPTGFESNSAVVGKGKNQEEVVTTSYAFQTAKLRQIRA 70

Query: 99  LNIE-SETVQVLDFAAFPEPEFDIPIFCANIFTAASSSIVVLDLNPLHDVISQRYYREKY 158
            +++   ++QVL+F  FP   +D+P F A++ T     ++ LD+ PL        Y+ KY
Sbjct: 71  AHVQGGNSLQVLNFVIFPHLNYDLPFFGADLVTLPGGHLIALDMQPLFR--DDSAYQAKY 130

Query: 159 YKSLLHLGTKYAELLPWGGKLTSESLKFFSPIVIWSKFSSTESKYDILFSAFMDYYKAWL 218
            + +L +   + + L WGG    E+  FFSP  +W++   T      +F+AF DY KA+L
Sbjct: 131 TEPILPIFHAHQQHLSWGGDFPEEAQPFFSPAFLWTRPQETAVVETQVFAAFKDYLKAYL 190

Query: 219 ELMNNTGEETIPPQLMVNREAQHRYLSWRAEKDPGHHLLRKLIGETLAKDVVRNFLF 269
           + +      T    L+  ++AQ RYL +RAEKDP   + ++  G    ++ +  FLF
Sbjct: 191 DFVEQAEAVTDSQNLVAIKQAQLRYLRYRAEKDPARGMFKRFYGAEWTEEYIHGFLF 245

BLAST of CSPI01G30700 vs. ExPASy Swiss-Prot
Match: Q7NL65 (Phycoerythrobilin:ferredoxin oxidoreductase OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=pebB PE=3 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.4e-24
Identity = 67/233 (28.76%), Postives = 115/233 (49.36%), Query Frame = 0

Query: 39  YQKFVQFALD--ETKLHTHLFPSPLQEKHNSMLSLDQKAKLEMLSFHAPKIRLLRSLNIE 98
           Y+ F++ A      +L    +P P+  +    L   +       ++ +PK+R +R+ +++
Sbjct: 4   YEPFLERAQQVLTRRLELAPYPIPVGFERKEALVRGEAVVTTSTAWQSPKLRQIRAAHVQ 63

Query: 99  -SETVQVLDFAAFPEPEFDIPIFCANIFTAASSSIVVLDLNPLHDVISQRYYREKYYKSL 158
               +QVL+F   P  E+D+P F A++ T     ++ LD+ PL        Y+ KY + +
Sbjct: 64  GGGALQVLNFVISPRLEYDLPFFGADLVTLPGGHLIALDMQPLFR--DDPAYQAKYTEPI 123

Query: 159 LHLGTKYAELLPWGGKLTSESLKFFSPIVIWSKFSSTESKYDILFSAFMDYYKAWLELMN 218
           + L   +   L WGG    E+  FFSP  +W++   T +    +F+AF+DY  A+L+ + 
Sbjct: 124 VPLFEAHRAHLEWGGDFPEEARPFFSPAFLWTRPKETGTVETRVFAAFVDYLNAYLDFVE 183

Query: 219 NTGEETIPPQLMVNREAQHRYLSWRAEKDPGHHLLRKLIGETLAKDVVRNFLF 269
                T P  L     AQ RYL +RAEKDP   + R+  G    ++ +  FLF
Sbjct: 184 RAEPVTHPEGLAAVERAQLRYLHYRAEKDPARGMFRRFYGPEWTEEYIHGFLF 234

BLAST of CSPI01G30700 vs. ExPASy Swiss-Prot
Match: Q7V585 (Phycoerythrobilin:ferredoxin oxidoreductase OS=Prochlorococcus marinus (strain MIT 9313) OX=74547 GN=pebB PE=3 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 4.9e-17
Identity = 57/188 (30.32%), Postives = 96/188 (51.06%), Query Frame = 0

Query: 86  KIRLLRSLNIES-ETVQVLDFAAFPEPEFDIPIFCANIFTAASSSIVVLDLNPLHDVISQ 145
           K R +R+  +E+  +  VL+F   P   FD+P F A++ T  S  ++ LDL P   + S 
Sbjct: 71  KFRQVRAACVEAGRSASVLNFVINPYHTFDLPFFGADLVTLPSGHLLALDLQPA--ITSD 130

Query: 146 RYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFFSPIVIWSKFS-STESKY---DILF 205
             + ++ ++ L+ +   +   LP GG +  E+  +FSP  +W++   S E      +++ 
Sbjct: 131 ERHTKQVWERLMPIFEHWRVHLPEGGPIPEEAKPYFSPGFLWTRLPLSIEGNQLIDEVIM 190

Query: 206 SAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWRAEKDPGHHLLRKLIGETLAK 265
            AF DY   +L+L+    EE  P +     E Q RYLS+RA+KDP   +L +  G    +
Sbjct: 191 PAFKDYLNLYLDLV-EMAEEVSPQRAFKLLEGQKRYLSYRAKKDPARAMLARFHGHQWTE 250

Query: 266 DVVRNFLF 269
             + N LF
Sbjct: 251 SYIHNVLF 255

BLAST of CSPI01G30700 vs. ExPASy TrEMBL
Match: A0A0A0LXG3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G616870 PE=3 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 2.2e-185
Identity = 320/322 (99.38%), Postives = 322/322 (100.00%), Query Frame = 0

Query: 1   MGSSRLCFSLSFNSPPFSTNCSSKNRSKRAFFPISSISYQKFVQFALDETKLHTHLFPSP 60
           MGSSRLCFSLSFNSPPFSTNCSSKNRSKRAFFPISSISYQKFVQFALDETKLHTHLFPSP
Sbjct: 1   MGSSRLCFSLSFNSPPFSTNCSSKNRSKRAFFPISSISYQKFVQFALDETKLHTHLFPSP 60

Query: 61  LQEKHNSMLSLDQKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCA 120
           LQEKHNSMLSLDQK+KLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCA
Sbjct: 61  LQEKHNSMLSLDQKSKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCA 120

Query: 121 NIFTAASSSIVVLDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFF 180
           NIFTAA+SSIVVLDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFF
Sbjct: 121 NIFTAANSSIVVLDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFF 180

Query: 181 SPIVIWSKFSSTESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWR 240
           SPIVIWSKFSSTESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWR
Sbjct: 181 SPIVIWSKFSSTESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWR 240

Query: 241 AEKDPGHHLLRKLIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSMA 300
           AEKDPGHHLLRKLIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSMA
Sbjct: 241 AEKDPGHHLLRKLIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSMA 300

Query: 301 GKSYEHRPWDERGEFIGDTIKT 323
           GKSYEHRPWDERGEFIGDTIKT
Sbjct: 301 GKSYEHRPWDERGEFIGDTIKT 322

BLAST of CSPI01G30700 vs. ExPASy TrEMBL
Match: A0A1S3BP29 (phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492001 PE=3 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.0e-175
Identity = 306/322 (95.03%), Postives = 314/322 (97.52%), Query Frame = 0

Query: 1   MGSSRLCFSLSFNSPPFSTNCSSKNRSKRAFFPISSISYQKFVQFALDETKLHTHLFPSP 60
           MGSSRL FSLSFNSPPFSTNC+SKNR KRA FPISSISYQKFVQFALDETKLHTHLFPSP
Sbjct: 1   MGSSRLSFSLSFNSPPFSTNCNSKNRFKRACFPISSISYQKFVQFALDETKLHTHLFPSP 60

Query: 61  LQEKHNSMLSLDQKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCA 120
           LQEKHNSMLSLDQKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCA
Sbjct: 61  LQEKHNSMLSLDQKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCA 120

Query: 121 NIFTAASSSIVVLDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFF 180
           NIFTAA+SSIVVLDLNPLHDVI+QRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFF
Sbjct: 121 NIFTAANSSIVVLDLNPLHDVITQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFF 180

Query: 181 SPIVIWSKFSSTESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWR 240
           SPIVIWSKFSSTESKYDIL SAFMDYYKAWLELMNNT EETIP QLM+NREAQHRYLSWR
Sbjct: 181 SPIVIWSKFSSTESKYDILSSAFMDYYKAWLELMNNTVEETIPSQLMINREAQHRYLSWR 240

Query: 241 AEKDPGHHLLRKLIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSMA 300
           AEKDPGHHLLRKLIGETLAKDVV+NFLF+G+DELGSK FLDYFPEY CEDGTINTKRSMA
Sbjct: 241 AEKDPGHHLLRKLIGETLAKDVVQNFLFNGIDELGSKSFLDYFPEYRCEDGTINTKRSMA 300

Query: 301 GKSYEHRPWDERGEFIGDTIKT 323
           GKSYEHRPWDERGEFIGDTIK+
Sbjct: 301 GKSYEHRPWDERGEFIGDTIKS 322

BLAST of CSPI01G30700 vs. ExPASy TrEMBL
Match: A0A6J1JIV1 (phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111485492 PE=3 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 4.3e-157
Identity = 272/319 (85.27%), Postives = 297/319 (93.10%), Query Frame = 0

Query: 4   SRLCFSLSFNSPPFSTNCSSKNRSKRAFFPISSISYQKFVQFALDETKLHTHLFPSPLQE 63
           S L F LSFNS PFS++CSSKN+ +RAFFP+S+ISYQKFVQFALDETKLHTHLFPSPLQE
Sbjct: 5   STLSFCLSFNSTPFSSSCSSKNKCRRAFFPVSAISYQKFVQFALDETKLHTHLFPSPLQE 64

Query: 64  KHNSMLSLDQKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCANIF 123
           K+NSM+S+D+  KL+MLSF APKIRLLRSLNIESET+QVLDFAAFPE EFD+PIFCANIF
Sbjct: 65  KYNSMISMDKNVKLQMLSFQAPKIRLLRSLNIESETMQVLDFAAFPETEFDVPIFCANIF 124

Query: 124 TAASSSIVVLDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFFSPI 183
           TAASSSIVVLDLNPLHDVI+QR YREKYYKSLLHLG KYAELLPWGGKLTSESLKFFSPI
Sbjct: 125 TAASSSIVVLDLNPLHDVINQRDYREKYYKSLLHLGIKYAELLPWGGKLTSESLKFFSPI 184

Query: 184 VIWSKFSSTESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWRAEK 243
           VIW+K SSTES YDILFSAF+DYYKAWLEL+NN  EETIP +LMVNREAQHRYLSWRAEK
Sbjct: 185 VIWTKSSSTESTYDILFSAFVDYYKAWLELVNNAAEETIPSRLMVNREAQHRYLSWRAEK 244

Query: 244 DPGHHLLRKLIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSMAGKS 303
           DPGH LL+KLIGE LAKD+VRNFLF+GVDELGSK+FLDYFPEY CEDGT+NTKRSMAGKS
Sbjct: 245 DPGHGLLKKLIGEMLAKDLVRNFLFNGVDELGSKRFLDYFPEYYCEDGTVNTKRSMAGKS 304

Query: 304 YEHRPWDERGEFIGDTIKT 323
           YEHRPWD+ GEFIGD  K+
Sbjct: 305 YEHRPWDKTGEFIGDLTKS 323

BLAST of CSPI01G30700 vs. ExPASy TrEMBL
Match: A0A6J1HBM1 (phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111461368 PE=3 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 2.8e-156
Identity = 270/319 (84.64%), Postives = 298/319 (93.42%), Query Frame = 0

Query: 4   SRLCFSLSFNSPPFSTNCSSKNRSKRAFFPISSISYQKFVQFALDETKLHTHLFPSPLQE 63
           S L F LSFNSP  S++CSSKN+ + AF P+S+ISY+KFVQFALDETKLHTHLFPSPLQE
Sbjct: 5   STLSFCLSFNSPLVSSSCSSKNKCRTAFIPVSAISYRKFVQFALDETKLHTHLFPSPLQE 64

Query: 64  KHNSMLSLDQKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCANIF 123
           K+NSM+S+D+K KLEMLSF APKIRLLRSLNIESET+QVLDFAAFPE EFD+PIFCANIF
Sbjct: 65  KYNSMISMDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPETEFDVPIFCANIF 124

Query: 124 TAASSSIVVLDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFFSPI 183
           TAASSSIVVLDLNPLHDVI+QR YREKYYKSL+HLGTKYAELLPWGGKLTSESLKFFSPI
Sbjct: 125 TAASSSIVVLDLNPLHDVINQRDYREKYYKSLMHLGTKYAELLPWGGKLTSESLKFFSPI 184

Query: 184 VIWSKFSSTESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWRAEK 243
           VIW+K SSTES YD+LFSAF+DYYKAWLEL++N  EETIP +LMVNREAQHRYLSWRAEK
Sbjct: 185 VIWTKSSSTESTYDVLFSAFVDYYKAWLELVHNAAEETIPSRLMVNREAQHRYLSWRAEK 244

Query: 244 DPGHHLLRKLIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSMAGKS 303
           DPGH LL+KLIGETLAKD+VRNFLF+GVDELGSK+FLDYFPEY CEDGT+NTKRSMAGKS
Sbjct: 245 DPGHGLLKKLIGETLAKDLVRNFLFNGVDELGSKRFLDYFPEYYCEDGTVNTKRSMAGKS 304

Query: 304 YEHRPWDERGEFIGDTIKT 323
           YEHRPWD+RGEFIGD  K+
Sbjct: 305 YEHRPWDKRGEFIGDLTKS 323

BLAST of CSPI01G30700 vs. ExPASy TrEMBL
Match: A0A6J1DJE9 (phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111021651 PE=3 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.5e-154
Identity = 266/320 (83.12%), Postives = 295/320 (92.19%), Query Frame = 0

Query: 4   SRLCFSLSFNSPPFSTNCSSKNRSKRA-FFPISSISYQKFVQFALDETKLHTHLFPSPLQ 63
           SRL F   FNSPPFS+NCS KN+ +RA F P+S+ISYQKFVQFALDETKLHTHL P+PLQ
Sbjct: 5   SRLSFCSPFNSPPFSSNCSGKNKCRRASFVPVSAISYQKFVQFALDETKLHTHLCPTPLQ 64

Query: 64  EKHNSMLSLDQKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCANI 123
           EK+NSM+S+D+KAKL+MLSF APKIRLLRSLNIESE +QVLDFA FP+PEFD+PIFCANI
Sbjct: 65  EKYNSMISMDKKAKLQMLSFQAPKIRLLRSLNIESEAMQVLDFAVFPKPEFDVPIFCANI 124

Query: 124 FTAASSSIVVLDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFFSP 183
           F+AASSSIVVLDLNPLHDVISQR Y+EKYY+SLLHLGTKYAELLPWGGKLTSESLKFFSP
Sbjct: 125 FSAASSSIVVLDLNPLHDVISQRDYKEKYYESLLHLGTKYAELLPWGGKLTSESLKFFSP 184

Query: 184 IVIWSKFSSTESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWRAE 243
           IVIW+KF ST SKYDILFSAF+DYYKAWLELMNN  EET P +LMVNREAQHRYLSWR E
Sbjct: 185 IVIWTKFPSTRSKYDILFSAFVDYYKAWLELMNNAREETNPSRLMVNREAQHRYLSWRVE 244

Query: 244 KDPGHHLLRKLIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSMAGK 303
           KDPGH LL++LIGE LAKD++RNFLF+GVDELGSK+FL+YFPEYCCEDGTIN KRSM GK
Sbjct: 245 KDPGHGLLKRLIGEALAKDLLRNFLFNGVDELGSKRFLEYFPEYCCEDGTINKKRSMVGK 304

Query: 304 SYEHRPWDERGEFIGDTIKT 323
           SYEHRPWD+RGEF+GD IKT
Sbjct: 305 SYEHRPWDKRGEFVGDLIKT 324

BLAST of CSPI01G30700 vs. NCBI nr
Match: XP_004139053.1 (phytochromobilin:ferredoxin oxidoreductase, chloroplastic [Cucumis sativus] >KGN66520.1 hypothetical protein Csa_007206 [Cucumis sativus])

HSP 1 Score: 657.9 bits (1696), Expect = 4.5e-185
Identity = 320/322 (99.38%), Postives = 322/322 (100.00%), Query Frame = 0

Query: 1   MGSSRLCFSLSFNSPPFSTNCSSKNRSKRAFFPISSISYQKFVQFALDETKLHTHLFPSP 60
           MGSSRLCFSLSFNSPPFSTNCSSKNRSKRAFFPISSISYQKFVQFALDETKLHTHLFPSP
Sbjct: 1   MGSSRLCFSLSFNSPPFSTNCSSKNRSKRAFFPISSISYQKFVQFALDETKLHTHLFPSP 60

Query: 61  LQEKHNSMLSLDQKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCA 120
           LQEKHNSMLSLDQK+KLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCA
Sbjct: 61  LQEKHNSMLSLDQKSKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCA 120

Query: 121 NIFTAASSSIVVLDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFF 180
           NIFTAA+SSIVVLDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFF
Sbjct: 121 NIFTAANSSIVVLDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFF 180

Query: 181 SPIVIWSKFSSTESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWR 240
           SPIVIWSKFSSTESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWR
Sbjct: 181 SPIVIWSKFSSTESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWR 240

Query: 241 AEKDPGHHLLRKLIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSMA 300
           AEKDPGHHLLRKLIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSMA
Sbjct: 241 AEKDPGHHLLRKLIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSMA 300

Query: 301 GKSYEHRPWDERGEFIGDTIKT 323
           GKSYEHRPWDERGEFIGDTIKT
Sbjct: 301 GKSYEHRPWDERGEFIGDTIKT 322

BLAST of CSPI01G30700 vs. NCBI nr
Match: XP_008450371.1 (PREDICTED: phytochromobilin:ferredoxin oxidoreductase, chloroplastic [Cucumis melo])

HSP 1 Score: 624.8 bits (1610), Expect = 4.2e-175
Identity = 306/322 (95.03%), Postives = 314/322 (97.52%), Query Frame = 0

Query: 1   MGSSRLCFSLSFNSPPFSTNCSSKNRSKRAFFPISSISYQKFVQFALDETKLHTHLFPSP 60
           MGSSRL FSLSFNSPPFSTNC+SKNR KRA FPISSISYQKFVQFALDETKLHTHLFPSP
Sbjct: 1   MGSSRLSFSLSFNSPPFSTNCNSKNRFKRACFPISSISYQKFVQFALDETKLHTHLFPSP 60

Query: 61  LQEKHNSMLSLDQKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCA 120
           LQEKHNSMLSLDQKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCA
Sbjct: 61  LQEKHNSMLSLDQKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCA 120

Query: 121 NIFTAASSSIVVLDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFF 180
           NIFTAA+SSIVVLDLNPLHDVI+QRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFF
Sbjct: 121 NIFTAANSSIVVLDLNPLHDVITQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFF 180

Query: 181 SPIVIWSKFSSTESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWR 240
           SPIVIWSKFSSTESKYDIL SAFMDYYKAWLELMNNT EETIP QLM+NREAQHRYLSWR
Sbjct: 181 SPIVIWSKFSSTESKYDILSSAFMDYYKAWLELMNNTVEETIPSQLMINREAQHRYLSWR 240

Query: 241 AEKDPGHHLLRKLIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSMA 300
           AEKDPGHHLLRKLIGETLAKDVV+NFLF+G+DELGSK FLDYFPEY CEDGTINTKRSMA
Sbjct: 241 AEKDPGHHLLRKLIGETLAKDVVQNFLFNGIDELGSKSFLDYFPEYRCEDGTINTKRSMA 300

Query: 301 GKSYEHRPWDERGEFIGDTIKT 323
           GKSYEHRPWDERGEFIGDTIK+
Sbjct: 301 GKSYEHRPWDERGEFIGDTIKS 322

BLAST of CSPI01G30700 vs. NCBI nr
Match: XP_038880083.1 (phytochromobilin:ferredoxin oxidoreductase, chloroplastic [Benincasa hispida])

HSP 1 Score: 598.6 bits (1542), Expect = 3.2e-167
Identity = 289/320 (90.31%), Postives = 308/320 (96.25%), Query Frame = 0

Query: 3   SSRLCFSLSFNSPPFSTNCSSKNRSKRAFFPISSISYQKFVQFALDETKLHTHLFPSPLQ 62
           SSRL FSLSFNSPPFSTNCSSKN+ KRAF PISSISYQKFVQFALDETKLHTHLFPSPLQ
Sbjct: 4   SSRLSFSLSFNSPPFSTNCSSKNKCKRAFLPISSISYQKFVQFALDETKLHTHLFPSPLQ 63

Query: 63  EKHNSMLSLDQKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCANI 122
           EK+NSM+SLD+K KLEMLSF APKIRLLRSLNIESET+QVLD AAFPEPEFD+PIFCANI
Sbjct: 64  EKYNSMISLDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDLAAFPEPEFDVPIFCANI 123

Query: 123 FTAASSSIVVLDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFFSP 182
           FTAASSSIVVLDLNPLHDVI+QR YREKYYKSLLHLGTKYAELLPWGGKLTSESLKFFSP
Sbjct: 124 FTAASSSIVVLDLNPLHDVITQRDYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFFSP 183

Query: 183 IVIWSKFSSTESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWRAE 242
           IVIWSKFSSTESKYDILFSAF+DYYKAWLELM+NTGEETIP +L++NREAQHRYLSWR E
Sbjct: 184 IVIWSKFSSTESKYDILFSAFVDYYKAWLELMDNTGEETIPSRLLINREAQHRYLSWREE 243

Query: 243 KDPGHHLLRKLIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSMAGK 302
           KDPGH LLRKLIGE LAKD+VRNFLF+G+DELGSK+FLDYFPEYCCEDG+INTKRSMAGK
Sbjct: 244 KDPGHGLLRKLIGEMLAKDLVRNFLFNGIDELGSKRFLDYFPEYCCEDGSINTKRSMAGK 303

Query: 303 SYEHRPWDERGEFIGDTIKT 323
           SYEHRPWD+ GEFIGDTIK+
Sbjct: 304 SYEHRPWDKTGEFIGDTIKS 323

BLAST of CSPI01G30700 vs. NCBI nr
Match: XP_023515585.1 (phytochromobilin:ferredoxin oxidoreductase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 575.5 bits (1482), Expect = 2.9e-160
Identity = 280/323 (86.69%), Postives = 305/323 (94.43%), Query Frame = 0

Query: 1   MGS-SRLCFSLSFNSPPFSTNCSSKNRSKRAFFPISSISYQKFVQFALDETKLHTHLFPS 60
           MGS S L F LSFNSPPFS++CSSKN+ +RAFFP+S+ISYQKFVQFALDETKLHTHLFPS
Sbjct: 1   MGSCSTLPFCLSFNSPPFSSSCSSKNKCRRAFFPVSAISYQKFVQFALDETKLHTHLFPS 60

Query: 61  PLQEKHNSMLSLDQKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFC 120
           PLQEK+NSM+S+D+K KLEMLSF APKIRLLRSLNIESET+QVLDFAAFPE EFD+PIFC
Sbjct: 61  PLQEKYNSMISMDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPETEFDVPIFC 120

Query: 121 ANIFTAASSSIVVLDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKF 180
           ANIFTAASSSIVVLDLNPLHDVI+QR YREKYYKS+LHLGTKYAELLPWGGKLTSESLKF
Sbjct: 121 ANIFTAASSSIVVLDLNPLHDVINQRDYREKYYKSILHLGTKYAELLPWGGKLTSESLKF 180

Query: 181 FSPIVIWSKFSSTESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSW 240
           FSPIVIW+K SSTES YDILFSAF+DYYKAWLEL+NN  EETIP +LMVNREAQHRYLSW
Sbjct: 181 FSPIVIWNKSSSTESTYDILFSAFVDYYKAWLELVNNAAEETIPFRLMVNREAQHRYLSW 240

Query: 241 RAEKDPGHHLLRKLIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSM 300
           RAEKDPGH LL+KLIGETLAKD+VRNFLF+GVDELGSK+FLDYFPEY CEDGT+NTKRSM
Sbjct: 241 RAEKDPGHGLLKKLIGETLAKDLVRNFLFNGVDELGSKRFLDYFPEYYCEDGTVNTKRSM 300

Query: 301 AGKSYEHRPWDERGEFIGDTIKT 323
           AGKSYEHRPWD+RGEFIGD  K+
Sbjct: 301 AGKSYEHRPWDKRGEFIGDLTKS 323

BLAST of CSPI01G30700 vs. NCBI nr
Match: XP_022988175.1 (phytochromobilin:ferredoxin oxidoreductase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 563.9 bits (1452), Expect = 8.8e-157
Identity = 272/319 (85.27%), Postives = 297/319 (93.10%), Query Frame = 0

Query: 4   SRLCFSLSFNSPPFSTNCSSKNRSKRAFFPISSISYQKFVQFALDETKLHTHLFPSPLQE 63
           S L F LSFNS PFS++CSSKN+ +RAFFP+S+ISYQKFVQFALDETKLHTHLFPSPLQE
Sbjct: 5   STLSFCLSFNSTPFSSSCSSKNKCRRAFFPVSAISYQKFVQFALDETKLHTHLFPSPLQE 64

Query: 64  KHNSMLSLDQKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCANIF 123
           K+NSM+S+D+  KL+MLSF APKIRLLRSLNIESET+QVLDFAAFPE EFD+PIFCANIF
Sbjct: 65  KYNSMISMDKNVKLQMLSFQAPKIRLLRSLNIESETMQVLDFAAFPETEFDVPIFCANIF 124

Query: 124 TAASSSIVVLDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFFSPI 183
           TAASSSIVVLDLNPLHDVI+QR YREKYYKSLLHLG KYAELLPWGGKLTSESLKFFSPI
Sbjct: 125 TAASSSIVVLDLNPLHDVINQRDYREKYYKSLLHLGIKYAELLPWGGKLTSESLKFFSPI 184

Query: 184 VIWSKFSSTESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWRAEK 243
           VIW+K SSTES YDILFSAF+DYYKAWLEL+NN  EETIP +LMVNREAQHRYLSWRAEK
Sbjct: 185 VIWTKSSSTESTYDILFSAFVDYYKAWLELVNNAAEETIPSRLMVNREAQHRYLSWRAEK 244

Query: 244 DPGHHLLRKLIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSMAGKS 303
           DPGH LL+KLIGE LAKD+VRNFLF+GVDELGSK+FLDYFPEY CEDGT+NTKRSMAGKS
Sbjct: 245 DPGHGLLKKLIGEMLAKDLVRNFLFNGVDELGSKRFLDYFPEYYCEDGTVNTKRSMAGKS 304

Query: 304 YEHRPWDERGEFIGDTIKT 323
           YEHRPWD+ GEFIGD  K+
Sbjct: 305 YEHRPWDKTGEFIGDLTKS 323

BLAST of CSPI01G30700 vs. TAIR 10
Match: AT3G09150.2 (phytochromobilin:ferredoxin oxidoreductase, chloroplast / phytochromobilin synthase (HY2) )

HSP 1 Score: 367.5 bits (942), Expect = 1.1e-101
Identity = 171/305 (56.07%), Postives = 232/305 (76.07%), Query Frame = 0

Query: 13  NSPPFSTNCSSKNRSKRAFFPISSISYQKFVQFALDETKLHTHLFPSPLQEKHNSMLSLD 72
           ++ P   N + + R KR    +S++SY++F + AL+ET+    L PS LQEK++SM  LD
Sbjct: 25  SASPNKINFTLRRRKKRFLLRVSAVSYKEFAESALEETRKRIVLEPSHLQEKYSSMTGLD 84

Query: 73  QKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCANIFTAASSSIVV 132
            K +L+ML+F + KIRLLRS+ IE+ET+QV DFA F EPE+D PIFCAN FT+ + +IVV
Sbjct: 85  GKTELQMLAFKSSKIRLLRSMAIENETMQVFDFAGFMEPEYDTPIFCANFFTSTNVNIVV 144

Query: 133 LDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFFSPIVIWSKFSST 192
           LDLNPLH +  Q  Y++KYY  ++ +  KYAE  PWGGKLT ES+KFFSP+V+W++FSS+
Sbjct: 145 LDLNPLHQLTDQTDYQDKYYNKIMSIYHKYAETFPWGGKLTGESIKFFSPLVMWTRFSSS 204

Query: 193 ESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWRAEKDPGHHLLRK 252
           + K+  LFSAF++YY+AWLE+     EE  P  +  N EAQH+YL+WRA+KDPGH LL++
Sbjct: 205 KEKHKALFSAFLEYYQAWLEMTIQVREEMEPSHVRANCEAQHKYLTWRAQKDPGHGLLKR 264

Query: 253 LIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSMAGKSYEHRPWDER 312
           L+GE  AK+++R+FLF+GVDELG+K F+DYFPEY  EDGT++ KRS+ GKSYE RPWD  
Sbjct: 265 LVGEAKAKELLRDFLFNGVDELGTKTFIDYFPEYQTEDGTVSDKRSIIGKSYETRPWDLT 324

Query: 313 GEFIG 318
           G+FIG
Sbjct: 325 GQFIG 329

BLAST of CSPI01G30700 vs. TAIR 10
Match: AT3G09150.1 (phytochromobilin:ferredoxin oxidoreductase, chloroplast / phytochromobilin synthase (HY2) )

HSP 1 Score: 358.6 bits (919), Expect = 5.2e-99
Identity = 169/305 (55.41%), Postives = 230/305 (75.41%), Query Frame = 0

Query: 13  NSPPFSTNCSSKNRSKRAFFPISSISYQKFVQFALDETKLHTHLFPSPLQEKHNSMLSLD 72
           ++ P   N + + R KR    +S++SY++F + AL+ET+    L PS LQ  ++SM  LD
Sbjct: 25  SASPNKINFTLRRRKKRFLLRVSAVSYKEFAESALEETRKRIVLEPSHLQ--YSSMTGLD 84

Query: 73  QKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCANIFTAASSSIVV 132
            K +L+ML+F + KIRLLRS+ IE+ET+QV DFA F EPE+D PIFCAN FT+ + +IVV
Sbjct: 85  GKTELQMLAFKSSKIRLLRSMAIENETMQVFDFAGFMEPEYDTPIFCANFFTSTNVNIVV 144

Query: 133 LDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFFSPIVIWSKFSST 192
           LDLNPLH +  Q  Y++KYY  ++ +  KYAE  PWGGKLT ES+KFFSP+V+W++FSS+
Sbjct: 145 LDLNPLHQLTDQTDYQDKYYNKIMSIYHKYAETFPWGGKLTGESIKFFSPLVMWTRFSSS 204

Query: 193 ESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWRAEKDPGHHLLRK 252
           + K+  LFSAF++YY+AWLE+     EE  P  +  N EAQH+YL+WRA+KDPGH LL++
Sbjct: 205 KEKHKALFSAFLEYYQAWLEMTIQVREEMEPSHVRANCEAQHKYLTWRAQKDPGHGLLKR 264

Query: 253 LIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSMAGKSYEHRPWDER 312
           L+GE  AK+++R+FLF+GVDELG+K F+DYFPEY  EDGT++ KRS+ GKSYE RPWD  
Sbjct: 265 LVGEAKAKELLRDFLFNGVDELGTKTFIDYFPEYQTEDGTVSDKRSIIGKSYETRPWDLT 324

Query: 313 GEFIG 318
           G+FIG
Sbjct: 325 GQFIG 327

BLAST of CSPI01G30700 vs. TAIR 10
Match: AT3G09150.3 (phytochromobilin:ferredoxin oxidoreductase, chloroplast / phytochromobilin synthase (HY2) )

HSP 1 Score: 330.5 bits (846), Expect = 1.5e-90
Identity = 150/250 (60.00%), Postives = 197/250 (78.80%), Query Frame = 0

Query: 68  MLSLDQKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCANIFTAAS 127
           M  LD K +L+ML+F + KIRLLRS+ IE+ET+QV DFA F EPE+D PIFCAN FT+ +
Sbjct: 1   MTGLDGKTELQMLAFKSSKIRLLRSMAIENETMQVFDFAGFMEPEYDTPIFCANFFTSTN 60

Query: 128 SSIVVLDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFFSPIVIWS 187
            +IVVLDLNPLH +  Q  Y++KYY  ++ +  KYAE  PWGGKLT ES+KFFSP+V+W+
Sbjct: 61  VNIVVLDLNPLHQLTDQTDYQDKYYNKIMSIYHKYAETFPWGGKLTGESIKFFSPLVMWT 120

Query: 188 KFSSTESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWRAEKDPGH 247
           +FSS++ K+  LFSAF++YY+AWLE+     EE  P  +  N EAQH+YL+WRA+KDPGH
Sbjct: 121 RFSSSKEKHKALFSAFLEYYQAWLEMTIQVREEMEPSHVRANCEAQHKYLTWRAQKDPGH 180

Query: 248 HLLRKLIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSMAGKSYEHR 307
            LL++L+GE  AK+++R+FLF+GVDELG+K F+DYFPEY  EDGT++ KRS+ GKSYE R
Sbjct: 181 GLLKRLVGEAKAKELLRDFLFNGVDELGTKTFIDYFPEYQTEDGTVSDKRSIIGKSYETR 240

Query: 308 PWDERGEFIG 318
           PWD  G+FIG
Sbjct: 241 PWDLTGQFIG 250

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SR431.6e-10056.07Phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Arabidopsis thalian... [more]
Q10UW62.5e-2930.80Phycoerythrobilin:ferredoxin oxidoreductase OS=Trichodesmium erythraeum (strain ... [more]
Q93TM82.6e-2629.11Phycoerythrobilin:ferredoxin oxidoreductase OS=Nostoc punctiforme (strain ATCC 2... [more]
Q7NL651.4e-2428.76Phycoerythrobilin:ferredoxin oxidoreductase OS=Gloeobacter violaceus (strain ATC... [more]
Q7V5854.9e-1730.32Phycoerythrobilin:ferredoxin oxidoreductase OS=Prochlorococcus marinus (strain M... [more]
Match NameE-valueIdentityDescription
A0A0A0LXG32.2e-18599.38Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G616870 PE=3 SV=1[more]
A0A1S3BP292.0e-17595.03phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Cucumis melo OX=365... [more]
A0A6J1JIV14.3e-15785.27phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Cucurbita maxima OX... [more]
A0A6J1HBM12.8e-15684.64phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Cucurbita moschata ... [more]
A0A6J1DJE91.5e-15483.13phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Momordica charantia... [more]
Match NameE-valueIdentityDescription
XP_004139053.14.5e-18599.38phytochromobilin:ferredoxin oxidoreductase, chloroplastic [Cucumis sativus] >KGN... [more]
XP_008450371.14.2e-17595.03PREDICTED: phytochromobilin:ferredoxin oxidoreductase, chloroplastic [Cucumis me... [more]
XP_038880083.13.2e-16790.31phytochromobilin:ferredoxin oxidoreductase, chloroplastic [Benincasa hispida][more]
XP_023515585.12.9e-16086.69phytochromobilin:ferredoxin oxidoreductase, chloroplastic [Cucurbita pepo subsp.... [more]
XP_022988175.18.8e-15785.27phytochromobilin:ferredoxin oxidoreductase, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G09150.21.1e-10156.07phytochromobilin:ferredoxin oxidoreductase, chloroplast / phytochromobilin synth... [more]
AT3G09150.15.2e-9955.41phytochromobilin:ferredoxin oxidoreductase, chloroplast / phytochromobilin synth... [more]
AT3G09150.31.5e-9060.00phytochromobilin:ferredoxin oxidoreductase, chloroplast / phytochromobilin synth... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.1500.20coord: 33..264
e-value: 1.2E-57
score: 197.4
NoneNo IPR availablePANTHERPTHR34557:SF1PHYTOCHROMOBILIN:FERREDOXIN OXIDOREDUCTASE, CHLOROPLASTICcoord: 9..278
IPR009249Ferredoxin-dependent bilin reductasePFAMPF05996Fe_bilin_redcoord: 50..260
e-value: 3.2E-47
score: 161.2
IPR009249Ferredoxin-dependent bilin reductasePANTHERPTHR34557PHYTOCHROMOBILIN:FERREDOXIN OXIDOREDUCTASE, CHLOROPLASTICcoord: 9..278

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G30700.1CSPI01G30700.1mRNA
CSPI01G30700.2CSPI01G30700.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010019 chloroplast-nucleus signaling pathway
biological_process GO:0010024 phytochromobilin biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0050897 cobalt ion binding
molecular_function GO:0050619 phytochromobilin:ferredoxin oxidoreductase activity
molecular_function GO:0016636 oxidoreductase activity, acting on the CH-CH group of donors, iron-sulfur protein as acceptor