CSPI01G30250 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G30250
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionE3 ubiquitin ligase BIG BROTHER-like
LocationChr1: 24744175 .. 24750175 (-)
RNA-Seq ExpressionCSPI01G30250
SyntenyCSPI01G30250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGATTTGATTAGATGAGATGGAATCTGTTGTTGCACGCAATCAAAGAAAACTGAGAAAAGAGTTAAAGAAAGCGAGATAAATTCTCTAAACCTTCGATCTAAATTGGCCGAAGAAACCTACAGAAACCAAACCAACCACAATCATTCAACCATAGACTCTCTCTCTCTTTTTTCCCATCAAAGTTGATCATTGTCTCTTTCTTTCTTTAATCCCTTCCCTCTTCCTTTCGAATCAATCCCCTTTTCCTTTTCCTTTTCTTCTTCTTTTTTTTCTCTTCTCCCATTTCATTTTCATCTAGTTTTCCGGCACCCCTTTTGTTTTTTTCCTTCATCTCTTTCACTTCTTCCTCCAATTTCGATTTTTTCTGCTGACCCACATGTTCTTTTGAGCTTTTCATTCTTGGGATTCGGCGTCGGAGCTGCTTGTTTGTCTTCTGCGGATCGCTCCGTCGTTGAAATGTGAGTTTCTAACCACCTACATTTTGGGTTTTCTTGTTTTGCTTCTGTTTGTGTTGAAGCGAGTGGTGGTGGGATTCCTTTGATTGTGTTTAATTTTGATTGAATAAGTGATATTTGGGGTTGAATGATTGCTTGTTTCATTTCCTTTTTTTTTCCAGCAATTGCGAAATGTAGAAGGGATTGTTAGGGAGTTTGAGTCCGATGGTACTCTTCCATTGTGTTTCCTTCGTTAATTGTTGCTTGGTTTTAAAAAATTTGGATTTTATCTATTTCTTTTTCTGGGATCTGGTAGACTAGAGATTTGCTGAGAAGTGGTTTTGGCTGTGGCAGATGGCTTTAGACGAAATGTCTCGTCTGATCTGTGTATATTGTGTATGGGTTTACTCTACTTTAATCTAGGACATTAGAAAACTTTAGGGGCTGATTTTTCATGATCTGTTTCTCACATTTAGGTGTAAAATAATACTTGGTTTGCTTATTTTTCTCTTCTGTTTCAAGTTGTATGTTGGAACCTCTAACGGTTTAGTTTGGTAAACTTTTGTAATGGGTTGGCTTTGCTGAACAGTTTGGAGTGGGAGTTTGTTCTTTTAGTTTATTGCCTGGAGTATTCATTTGGTTTTGTGATGTTTCTGCATTTTACAGAATATTTGTTAACTTCAGTGTTAACTTCAGTTGTGTATGAAATGAGTTGGAACAGCACGCCGCAGACAGAAGTTCATCATGTTAACACCAATTATCCTTATAACACTGCTGGAAGTTTTCTGGAATACTTTGAAGGACTTACCTATGAACATGTAAATTTCATTTTTTCGGGTGCTTCACATTCTCAGGTACTTTAAATCTGCTTGCTTCTGTGTTCCAAACAACTGAACTTGAATTTTTTCCTTGCTATTGAACTATTCAATTGATTATTTATTTATTTTTATTTTTTTAATCTTCTCTGATGTAACTTTACGGACAACATCTACTTTACAGCACATGATTATTTTCTTGTTTGTGAAATGAAAGGTTGTGGTATATGCATCTTTTTGAGGGGCAGTAACATTTATTCTATAGGGAGTGATCTTGGGAAAGTTTCTTTGCATTTCTTTGGTTTTTTCTTCGTCTGTCCTTCCTTCTTGGGTTTTCTTTCAAGTTGAAATTGTCTTCCTCTCTTTATAATTTATAAGCTGGCAAATACTATACTTGAACGTAGTGCTCCTCTCTACCGTTGCTTTAATTAGTTTTGACTGTGAATGGAAAATGGCAATATACTTATTAAGTTTCACACTTGCAGGAGAATGTTTATCCTCCACTGAATTCAAACTTTTACAAGTTTGGGTTTTCAGATTTTGGGAGTCCTTCCTATTACAACCCACCTCAAGCTTATAGGATGCATGATCACGAGCCCATAATTTATGAACATAGAAGGCTAGAAAATTCTGCAACTCATCCTAATTTACAGAGTACCGTAAATCCTGAATTCGAAGAAATTACAAATACTATAGCATTTGACAACCATGTAGAATGTAAGTGATGATTCTCGGTAATTAGATAATTCTCACCATTCTCATCTCCCCCCCCCCCCCGGACCCGCCACTGTACACACATTAATCATATTATCTCATAAAAAATTAATCAATTAATTACATATACAACGAAATGGGTTTTTTGTTTATTAACTTGGAAGAAGAAGAAGGAGAAGAAGCATTCTGAATATTATTGGCAGCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCCCCCCCCCAAAAAAAAAAAAAAAAAAAAGAAAAGAAAAAAAAGAGTTCGTTTCAATTTTTTTTGTATTTGTTGGGTTCACCATTGAGATTTCCTAGAAGTACGCAAAATATTTACTTTTGCAATTGTAACAATTGTCTTGGTAGGAGTGAAACACCAAACACTGAAGGGGTTTTTTTCTAATATAAAAACGTAATTTTATCATTAACATTTGCCAGCATAGGGAATATCAGTATCTTTGTACTTATTTCTCAAGAGTTCAGTGCAGGCTGAATGTTACATCTTGAGAGAACACTTCATACATGAATGAACTAAGAATTCTGGTAGAAGATTCTTATAAGTTTTGTTTAGTTAAATACCAAGAAGAAATATGGTTCTTGAAATAAGATGACCCTTGAATTTTAGCTATTGGTTTATCTCTAAATGCTGCATATTTTCCCTCTCTGTTAGCACTTTTTTCGACTCTTGCTGTTAGCACTAACAACAGATTTTCTCTCAGGTCCAAGGAGACATCATAATTCACATGATTTTCAGGTTTGTGGCTTTTTACATGGTTTGACAAGTCAACTTTCTACTTGAAATACGACACTTGTTTGGTCGTGTGTTTTGGCCAAAATTATTGATGAACGAATATTTATGTTTACGTGTAGGTTGTTTGGCAAGACAATGTAGATCCTGATAATATGACTTATGAGGTTAGTGCATTTGATAAACTTTTTGTGTACTTGTTTTTTCCTTTTATTTGTTGGTGAAAGTATCGATTTCCATCAATATATGAAACCACATAGTCGGAAGAACCAAGTAATGCAAGTTACAATGATTGTGTCATCTCCTCAATTTATGGGGACGAATAAACCATGGGAGTTTTAATATCAATTTTATGATTGATTAGCAGATGCTGAAAGCTACCTTTGATATTGCATAAGAATTTAAACTTGATGTAATAGAATAACTGTAAGGAACTTGGGGAAAAAATTCTTGGTTGAGGATAGGCCAGAATGAGATGATTGATGAGACATTAAAATATGTAAGCTTAAGAAAGTTGTTCTTCCAAACCAAGCATAGTCAGCTGGTATCCTCAACCTAGAGATCAGAGGTTCAAATCTCTTGCCCCATGTTATTGAACTAAAAAAATTAGTTTAATAAGGATCCTGGTGCTTTATGATAGAGCTGCTGAACTATTATTCTGGGGTCAAGTTTTTATGTTTGTTTTTTGAACTAATGTTAAACAAATTTCTCCATCTGTCATCTGACCTAATCGTCCAAAATGTATGTTTATCTTTTCTCAATATTTGAATAAAAATCTATAATATTTCAAGTTCAGGAATTACTAGAGTTGGGTGAGACAGTTGGAACTCAAAGCCGAGGTCTATCTCAAGAATTGATCGCTTTGCTTCCGATATCGAAGTACAAATATAGTTTCTTCTCAAGGAAAAAGTCACGAGGAGAGAGGTATATCTTAGCTTATAATGTGCATTTGATGGAATGTGTTAAACTTTTTTCCTTTCCTTTCCTTTCGTTGCCACTAACATTGAGCTGTAATCATTATCCTCACATTTTAGTACTTAAGTAGTGGTGTTGGCAAGTTAATTTCTTAGTGATACAAGTTCTCTGACGAGCGAGACTTTGTAGCCTGTTTTATGCTAGTTTCATCTTGAATCAGTGATACGGGAAACAAAATTTGATTATCAGGCATTCAGATATGGCTTTCTTTATAAAAAAGGGGGCATTCTGATAATTTCATGTCAGACATACGTCTAAACTATGTTTTACTCATTCTTTCGCTCCATCAAGACAAGAAAGAGAATGGCAGCATGTATATTTTTGTCTCTGAATTGATGATTTATTAGGCTTATTCCCATGATAAAAGACTTATCAATTTATGAGAGCTCACAATTGCCTTTGACGTTTGATAGATTGCCATACTCTACTGAAAGATCTTTTTTATCTTTTTTTTTTTGAGATATGTTATCCAAGTATTCAAATAACAATATGTACCTTAACTTCTAAATTATGTCTTGTACTTGCGGAAAGATGCTAAATGAACATTTTGGACTTGGGCGTTTCTATTATAAAAAACTGAAACTGGTTGAGAGTTCAGTTTGTTCTTTTTCCTTTTGAGTCTAACTTTGTTCCTCTTAATTGGATGTAGATGTGTGATATGCCAGATGGAATATAAACGAGGTGATCGACGGATCACTCTGCCGTGCAAACATATCTACCATGCTGGTTGTGGAACCAAATGGCTTAGCATCAACAAGGTATTATTATTAGTTCATTGCATGTGCTGGGATTGTTGATTGTTTTATTGGTTACAACTCGCAGACTGATTCTTACGCTGTTCAAGATATCATAACAAGTAATAACCATGTTCATTCTGCCGAGGTCTTAAAAACGGCTTTATTAGAAACAAAATTCTAAAAACTACTTTCCCATTTCCAAACCAGGGCTTGTTTTATTTGTGTTTATAAGCTTAACTTTCAAAAATAAAAAACAAAAAGTCAAATTGTTGTCAAATGAGGCCTTAATTTGGTTTTTGGTTTTGAAAATTAAGCTTCTAAAAACTACTTCCACTTCAAGATTTCTTGGTTATGTGATTTGCTTTTTATAGATGTTTTTAAAGTCCAAATTCAGTTTAAAAAATTAAGGCTTTTTTGGATAATCTTTTTTTTTTTTTGGTTTTTTGAAAATTTCTCCCAATTTTTTAGTCATGATTTTCATATTTTTGTTTATGTAATTGTATAAATTCTTAGCCAATTTCTAGAAAACGAAACAATTTCTGAAACCGCTTCCTTTTAGTTTTCAGGTTTTAGATTTGATTTTGAAAAGGCTCCTACAATAGATAAAACAAACCATAAAAACCAATAGATGGAACTTGGAAGTGGTGCCTATAGGTTTAATTGTGGAGGTTACCGAACAAAACTTAAATAAAGTAGTTTTTGTCTTTGAAAGTTGATTAACAATTCAAATGTTCCTCAAATGTTACTCAAGGATTAGAAAACCACGATAAATAAATGGTGAGAAAGTGAATACAATTTTCAAAAAACCAAAAACCAAAAGGGACTCAAAATTTCTGATAGAGCTATTTTTTAAATGACTTGTAAAGGCTGAAGTTATTATGCACTTAGATCATTGTTTAGAGCTCCACAATCTTCTAAAGAAACCAATGAAAAACAACTTTCAGAAAACAATTTTGAAACCCAACTCTGAAGCAACTTCAGTTCCTGTCTTTAATAAAGTAAGGATGAAGATGATCTTTAATTGATTCTTCACCTTTTCTTTTTGGTGAGGCAGATCCAGAAACAGTCTTTTTGATTGAGTAATTAATTTCTATTGTATGGTTTTGTTGTACAGGCCTGCCCCATATGTTACACAGAGGTATTTGGTGATACTTCAAAACGCTAAGTCTTCTTCTCACTTCACTGTGTTAGATGACAGAAGAACATAATCTTTGTTATTGTTCATTATTTTTAAAATTTTTGTGAAAAAGAAAGAAAGAAAGAATATATATATATATTTTTATATATTTATATATATGGAGTGGCGCAAGGCAATCACTTGATTTTTCTTTCTTAAATATTTCCACTTTAGTAATATGGCAGTGCTGAAGTGAATAAAGATTAATTCTCCTTGTATTAGCTTTTATCAGTGTTTGGTGTTTGGATTGGCTTTTTCTTTTTTTCTTTCCCTTTTTCCTTTTTATTTCTTATTTTTTGGCAAAGGTAAGAGATGTGTAAGGATTGGTAAGTTTAGGAAGGGGAAACAATTAGTTTCTTTAGTTTCTTATGTATCCATGAA

mRNA sequence

GAAGATTTGATTAGATGAGATGGAATCTGTTGTTGCACGCAATCAAAGAAAACTGAGAAAAGAGTTAAAGAAAGCGAGATAAATTCTCTAAACCTTCGATCTAAATTGGCCGAAGAAACCTACAGAAACCAAACCAACCACAATCATTCAACCATAGACTCTCTCTCTCTTTTTTCCCATCAAAGTTGATCATTGTCTCTTTCTTTCTTTAATCCCTTCCCTCTTCCTTTCGAATCAATCCCCTTTTCCTTTTCCTTTTCTTCTTCTTTTTTTTCTCTTCTCCCATTTCATTTTCATCTAGTTTTCCGGCACCCCTTTTGTTTTTTTCCTTCATCTCTTTCACTTCTTCCTCCAATTTCGATTTTTTCTGCTGACCCACATGTTCTTTTGAGCTTTTCATTCTTGGGATTCGGCGTCGGAGCTGCTTGTTTGTCTTCTGCGGATCGCTCCGTCGTTGAAATAATATTTGTTAACTTCAGTGTTAACTTCAGTTGTGTATGAAATGAGTTGGAACAGCACGCCGCAGACAGAAGTTCATCATGTTAACACCAATTATCCTTATAACACTGCTGGAAGTTTTCTGGAATACTTTGAAGGACTTACCTATGAACATGTAAATTTCATTTTTTCGGGTGCTTCACATTCTCAGGAGAATGTTTATCCTCCACTGAATTCAAACTTTTACAAGTTTGGGTTTTCAGATTTTGGGAGTCCTTCCTATTACAACCCACCTCAAGCTTATAGGATGCATGATCACGAGCCCATAATTTATGAACATAGAAGGCTAGAAAATTCTGCAACTCATCCTAATTTACAGAGTACCGTAAATCCTGAATTCGAAGAAATTACAAATACTATAGCATTTGACAACCATGTAGAATGTCCAAGGAGACATCATAATTCACATGATTTTCAGGTTGTTTGGCAAGACAATGTAGATCCTGATAATATGACTTATGAGGAATTACTAGAGTTGGGTGAGACAGTTGGAACTCAAAGCCGAGGTCTATCTCAAGAATTGATCGCTTTGCTTCCGATATCGAAGTACAAATATAGTTTCTTCTCAAGGAAAAAGTCACGAGGAGAGAGATGTGTGATATGCCAGATGGAATATAAACGAGGTGATCGACGGATCACTCTGCCGTGCAAACATATCTACCATGCTGGTTGTGGAACCAAATGGCTTAGCATCAACAAGGCCTGCCCCATATGTTACACAGAGGTATTTGGTGATACTTCAAAACGCTAAGTCTTCTTCTCACTTCACTGTGTTAGATGACAGAAGAACATAATCTTTGTTATTGTTCATTATTTTTAAAATTTTTGTGAAAAAGAAAGAAAGAAAGAATATATATATATATTTTTATATATTTATATATATGGAGTGGCGCAAGGCAATCACTTGATTTTTCTTTCTTAAATATTTCCACTTTAGTAATATGGCAGTGCTGAAGTGAATAAAGATTAATTCTCCTTGTATTAGCTTTTATCAGTGTTTGGTGTTTGGATTGGCTTTTTCTTTTTTTCTTTCCCTTTTTCCTTTTTATTTCTTATTTTTTGGCAAAGGTAAGAGATGTGTAAGGATTGGTAAGTTTAGGAAGGGGAAACAATTAGTTTCTTTAGTTTCTTATGTATCCATGAA

Coding sequence (CDS)

ATGAGTTGGAACAGCACGCCGCAGACAGAAGTTCATCATGTTAACACCAATTATCCTTATAACACTGCTGGAAGTTTTCTGGAATACTTTGAAGGACTTACCTATGAACATGTAAATTTCATTTTTTCGGGTGCTTCACATTCTCAGGAGAATGTTTATCCTCCACTGAATTCAAACTTTTACAAGTTTGGGTTTTCAGATTTTGGGAGTCCTTCCTATTACAACCCACCTCAAGCTTATAGGATGCATGATCACGAGCCCATAATTTATGAACATAGAAGGCTAGAAAATTCTGCAACTCATCCTAATTTACAGAGTACCGTAAATCCTGAATTCGAAGAAATTACAAATACTATAGCATTTGACAACCATGTAGAATGTCCAAGGAGACATCATAATTCACATGATTTTCAGGTTGTTTGGCAAGACAATGTAGATCCTGATAATATGACTTATGAGGAATTACTAGAGTTGGGTGAGACAGTTGGAACTCAAAGCCGAGGTCTATCTCAAGAATTGATCGCTTTGCTTCCGATATCGAAGTACAAATATAGTTTCTTCTCAAGGAAAAAGTCACGAGGAGAGAGATGTGTGATATGCCAGATGGAATATAAACGAGGTGATCGACGGATCACTCTGCCGTGCAAACATATCTACCATGCTGGTTGTGGAACCAAATGGCTTAGCATCAACAAGGCCTGCCCCATATGTTACACAGAGGTATTTGGTGATACTTCAAAACGCTAA

Protein sequence

MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNFYKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATHPNLQSTVNPEFEEITNTIAFDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPISKYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTEVFGDTSKR*
Homology
BLAST of CSPI01G30250 vs. ExPASy Swiss-Prot
Match: Q8L649 (E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE=1 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 7.0e-56
Identity = 114/252 (45.24%), Postives = 160/252 (63.49%), Query Frame = 0

Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           M+ ++ P  + H+  T +PY   GS+++++ G     +N+  +   H Q+N+Y  +N+N 
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPLNYDHAATMHPQDNLYWTMNTNA 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATHPNLQSTVNPE-----FEEI 120
           YKFGFS   + S+Y    +Y M+DH   +   R   N   HP +    +PE       +I
Sbjct: 61  YKFGFSGSDNASFYG---SYDMNDHLSRMSIGR--TNWDYHPMVNVADDPENTVARSVQI 120

Query: 121 TNTIAFDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIA 180
            +T       EC    H+    QV WQD++DPD MTYEEL+ELGE VGT+SRGLSQELI 
Sbjct: 121 GDTDEHSEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIE 180

Query: 181 LLPISKYKY-SFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKAC 240
            LP  KYK+ S FSRK++ GERCVICQ++YK G+R++ LPCKH+YH+ C +KWLSINK C
Sbjct: 181 TLPTKKYKFGSIFSRKRA-GERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVC 240

Query: 241 PICYTEVFGDTS 247
           P+C +EVFG+ S
Sbjct: 241 PVCNSEVFGEPS 246

BLAST of CSPI01G30250 vs. ExPASy Swiss-Prot
Match: Q9LT17 (E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.3e-25
Identity = 56/113 (49.56%), Postives = 71/113 (62.83%), Query Frame = 0

Query: 134 SHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPISKYKYSFFSRKKSR 193
           SH  Q  W D +DPD ++YEELL LG+ VGT+SRGLS + IA LP  +YK      +   
Sbjct: 228 SHTSQDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEG--DNQNGT 287

Query: 194 GERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTEVFGDTS 247
            E CVIC+++Y+  +  I LPCKH YH+ C   WL INK CP+C  EV   TS
Sbjct: 288 NESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEVSTSTS 337

BLAST of CSPI01G30250 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.6e-15
Identity = 45/100 (45.00%), Postives = 58/100 (58.00%), Query Frame = 0

Query: 145 VDPDNMTYEELLELGETVGTQSRGLSQELIALLPISKYKYSFFSRKK----SRGERCVIC 204
           +D DNM+YEELL LGE +G  S GLS+E+I L  + ++K++  S          E C IC
Sbjct: 601 LDVDNMSYEELLALGERIGDVSTGLSEEVI-LKAMKQHKHTSSSPSSVELHQNIEPCCIC 660

Query: 205 QMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 241
           Q EY  GD   TL C H +H  C  +W+ I   CPIC TE
Sbjct: 661 QEEYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTE 699

BLAST of CSPI01G30250 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 3.5e-15
Identity = 42/96 (43.75%), Postives = 58/96 (60.42%), Query Frame = 0

Query: 145 VDPDNMTYEELLELGETVGTQSRGLSQELIALLPISKYKYSFFSRKKSRG-ERCVICQME 204
           +D DNM+YEELL LGE +G  S GLS+E+I L  + ++K++  +    +  E C +CQ E
Sbjct: 567 LDVDNMSYEELLALGERIGDVSTGLSEEVI-LKVMKQHKHTSSAAGSHQDMEPCCVCQEE 626

Query: 205 YKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYT 240
           Y  GD   TL C H +H  C  +WL +   CPIC T
Sbjct: 627 YAEGDDLGTLGCGHEFHTACVKQWLMLKNLCPICKT 661

BLAST of CSPI01G30250 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 82.4 bits (202), Expect = 7.9e-15
Identity = 43/94 (45.74%), Postives = 56/94 (59.57%), Query Frame = 0

Query: 145 VDPDNMTYEELLELGETVGTQSRGLSQELIALLPISKYKYSFFSRKKS-RGERCVICQME 204
           +D DNM+YEELL L E +G  S GLS+E +  L + + K+S +  + S   E C ICQ E
Sbjct: 568 LDIDNMSYEELLALEERIGNVSTGLSEEEVTKL-LKQRKFSSWRLEASVEEEPCCICQEE 627

Query: 205 YKRGDRRITLPCKHIYHAGCGTKWLSINKACPIC 238
           Y  GD   TL C H +H GC  +WL +   CPIC
Sbjct: 628 YVDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPIC 660

BLAST of CSPI01G30250 vs. ExPASy TrEMBL
Match: A0A0A0LX98 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G613470 PE=4 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 2.6e-146
Identity = 247/248 (99.60%), Postives = 247/248 (99.60%), Query Frame = 0

Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF
Sbjct: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATHPNLQSTVNPEFEEITNTIA 120
           YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSAT PNLQSTVNPEFEEITNTIA
Sbjct: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQSTVNPEFEEITNTIA 120

Query: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180
           FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS
Sbjct: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180

Query: 181 KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240
           KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE
Sbjct: 181 KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240

Query: 241 VFGDTSKR 249
           VFGDTSKR
Sbjct: 241 VFGDTSKR 248

BLAST of CSPI01G30250 vs. ExPASy TrEMBL
Match: A0A1S3BQ88 (E3 ubiquitin ligase BIG BROTHER-like OS=Cucumis melo OX=3656 GN=LOC103492041 PE=4 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 3.8e-145
Identity = 244/248 (98.39%), Postives = 246/248 (99.19%), Query Frame = 0

Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           MSWN+TPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF
Sbjct: 1   MSWNNTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATHPNLQSTVNPEFEEITNTIA 120
           YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSAT PNLQSTVNPEFEEITNTIA
Sbjct: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQSTVNPEFEEITNTIA 120

Query: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180
           FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS
Sbjct: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180

Query: 181 KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240
           KYKY FFSRKKSRG+RCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE
Sbjct: 181 KYKYGFFSRKKSRGDRCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240

Query: 241 VFGDTSKR 249
           VFGDTSKR
Sbjct: 241 VFGDTSKR 248

BLAST of CSPI01G30250 vs. ExPASy TrEMBL
Match: A0A6J1JIX4 (E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita maxima OX=3661 GN=LOC111485509 PE=4 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 2.3e-134
Identity = 228/248 (91.94%), Postives = 237/248 (95.56%), Query Frame = 0

Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           MSWN+T  TEVHHVNT++PYNTAGSFLEYFEGLTYEHVNFIFS ASHSQENVYPPLNSNF
Sbjct: 1   MSWNNT-HTEVHHVNTSHPYNTAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATHPNLQSTVNPEFEEITNTIA 120
           YKFGFSDFGSP+YYN PQAYRMHDHEP+IYEHRRLEN +T PN+QSTVNPEFEEI NT A
Sbjct: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEFEEIANTTA 120

Query: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180
           FDNHVECPRRH NSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIAL+PIS
Sbjct: 121 FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS 180

Query: 181 KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240
           KYKYSFFSRKK RGERCVICQMEYKRGDRR+TLPCKHIYHAGCGTKWLSINKACPICY E
Sbjct: 181 KYKYSFFSRKKFRGERCVICQMEYKRGDRRMTLPCKHIYHAGCGTKWLSINKACPICYKE 240

Query: 241 VFGDTSKR 249
           VFGDTSKR
Sbjct: 241 VFGDTSKR 247

BLAST of CSPI01G30250 vs. ExPASy TrEMBL
Match: A0A6J1HC34 (E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita moschata OX=3662 GN=LOC111461475 PE=4 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 2.3e-134
Identity = 226/248 (91.13%), Postives = 237/248 (95.56%), Query Frame = 0

Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           MSWN+T   EVHHVNT++PYN+AGSFLEYFEGLTYEHVNFIFS ASHSQENVYPPLNSNF
Sbjct: 1   MSWNNTHTEEVHHVNTSHPYNSAGSFLEYFEGLTYEHVNFIFSDASHSQENVYPPLNSNF 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATHPNLQSTVNPEFEEITNTIA 120
           YKFGFSDFGSP+YYN PQAYRMHDHEP+IYEHRRLEN +T PN+QSTVNPEFEEI+NT A
Sbjct: 61  YKFGFSDFGSPTYYNQPQAYRMHDHEPVIYEHRRLENFSTQPNVQSTVNPEFEEISNTTA 120

Query: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180
           FDNHVECPRRH NSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIAL+PIS
Sbjct: 121 FDNHVECPRRHRNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALIPIS 180

Query: 181 KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240
           KYKYSFFSRKK RGERCVICQM+YKRGDRRITLPCKHIYHAGCGTKWLSINKACPICY E
Sbjct: 181 KYKYSFFSRKKFRGERCVICQMDYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYKE 240

Query: 241 VFGDTSKR 249
           VFGDTSKR
Sbjct: 241 VFGDTSKR 248

BLAST of CSPI01G30250 vs. ExPASy TrEMBL
Match: A0A5A7UF61 (E3 ubiquitin ligase BIG BROTHER-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold773G00460 PE=4 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 3.9e-134
Identity = 228/232 (98.28%), Postives = 230/232 (99.14%), Query Frame = 0

Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           MSWN+TPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF
Sbjct: 36  MSWNNTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 95

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATHPNLQSTVNPEFEEITNTIA 120
           YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSAT PNLQSTVNPEFEEITNTIA
Sbjct: 96  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQSTVNPEFEEITNTIA 155

Query: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180
           FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS
Sbjct: 156 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 215

Query: 181 KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINK 233
           KYKY FFSRKKSRG+RCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINK
Sbjct: 216 KYKYGFFSRKKSRGDRCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINK 267

BLAST of CSPI01G30250 vs. NCBI nr
Match: XP_011660125.1 (E3 ubiquitin-protein ligase BIG BROTHER [Cucumis sativus] >XP_011660126.1 E3 ubiquitin-protein ligase BIG BROTHER [Cucumis sativus])

HSP 1 Score: 527.7 bits (1358), Expect = 5.4e-146
Identity = 247/248 (99.60%), Postives = 247/248 (99.60%), Query Frame = 0

Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF
Sbjct: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATHPNLQSTVNPEFEEITNTIA 120
           YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSAT PNLQSTVNPEFEEITNTIA
Sbjct: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQSTVNPEFEEITNTIA 120

Query: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180
           FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS
Sbjct: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180

Query: 181 KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240
           KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE
Sbjct: 181 KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240

Query: 241 VFGDTSKR 249
           VFGDTSKR
Sbjct: 241 VFGDTSKR 248

BLAST of CSPI01G30250 vs. NCBI nr
Match: XP_008450441.1 (PREDICTED: E3 ubiquitin ligase BIG BROTHER-like [Cucumis melo] >XP_008450442.1 PREDICTED: E3 ubiquitin ligase BIG BROTHER-like [Cucumis melo])

HSP 1 Score: 523.9 bits (1348), Expect = 7.8e-145
Identity = 244/248 (98.39%), Postives = 246/248 (99.19%), Query Frame = 0

Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           MSWN+TPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF
Sbjct: 1   MSWNNTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATHPNLQSTVNPEFEEITNTIA 120
           YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSAT PNLQSTVNPEFEEITNTIA
Sbjct: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQSTVNPEFEEITNTIA 120

Query: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180
           FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS
Sbjct: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180

Query: 181 KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240
           KYKY FFSRKKSRG+RCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE
Sbjct: 181 KYKYGFFSRKKSRGDRCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240

Query: 241 VFGDTSKR 249
           VFGDTSKR
Sbjct: 241 VFGDTSKR 248

BLAST of CSPI01G30250 vs. NCBI nr
Match: XP_038880080.1 (E3 ubiquitin-protein ligase BIG BROTHER-like isoform X2 [Benincasa hispida])

HSP 1 Score: 512.7 bits (1319), Expect = 1.8e-141
Identity = 241/248 (97.18%), Postives = 245/248 (98.79%), Query Frame = 0

Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           MSWN+T QTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF
Sbjct: 31  MSWNNT-QTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 90

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATHPNLQSTVNPEFEEITNTIA 120
           YKFGFSDFGSP+YYN PQAYRMHDHEPIIYEHRRLE+SAT PNLQST+NPEFEEITNTIA
Sbjct: 91  YKFGFSDFGSPAYYNQPQAYRMHDHEPIIYEHRRLESSATQPNLQSTINPEFEEITNTIA 150

Query: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180
           FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS
Sbjct: 151 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 210

Query: 181 KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240
           KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE
Sbjct: 211 KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 270

Query: 241 VFGDTSKR 249
           VFGDTSKR
Sbjct: 271 VFGDTSKR 277

BLAST of CSPI01G30250 vs. NCBI nr
Match: XP_038880079.1 (E3 ubiquitin-protein ligase BIG BROTHER-like isoform X1 [Benincasa hispida])

HSP 1 Score: 501.1 bits (1289), Expect = 5.4e-138
Identity = 240/256 (93.75%), Postives = 244/256 (95.31%), Query Frame = 0

Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           MSWN+T QTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF
Sbjct: 31  MSWNNT-QTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 90

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATHPNLQSTVNPEFEEITNTIA 120
           YKFGFSDFGSP+YYN PQAYRMHDHEPIIYEHRRLE+SAT PNLQST+NPEFEEITNTIA
Sbjct: 91  YKFGFSDFGSPAYYNQPQAYRMHDHEPIIYEHRRLESSATQPNLQSTINPEFEEITNTIA 150

Query: 121 FDNHVEC--------PRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQE 180
           FDNHVE         PRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQE
Sbjct: 151 FDNHVEFLITDFLSGPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQE 210

Query: 181 LIALLPISKYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINK 240
           LIALLPISKYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINK
Sbjct: 211 LIALLPISKYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINK 270

Query: 241 ACPICYTEVFGDTSKR 249
           ACPICYTEVFGDTSKR
Sbjct: 271 ACPICYTEVFGDTSKR 285

BLAST of CSPI01G30250 vs. NCBI nr
Match: XP_038880082.1 (E3 ubiquitin-protein ligase BIG BROTHER-like isoform X3 [Benincasa hispida])

HSP 1 Score: 501.1 bits (1289), Expect = 5.4e-138
Identity = 240/256 (93.75%), Postives = 244/256 (95.31%), Query Frame = 0

Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           MSWN+T QTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF
Sbjct: 1   MSWNNT-QTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATHPNLQSTVNPEFEEITNTIA 120
           YKFGFSDFGSP+YYN PQAYRMHDHEPIIYEHRRLE+SAT PNLQST+NPEFEEITNTIA
Sbjct: 61  YKFGFSDFGSPAYYNQPQAYRMHDHEPIIYEHRRLESSATQPNLQSTINPEFEEITNTIA 120

Query: 121 FDNHVEC--------PRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQE 180
           FDNHVE         PRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQE
Sbjct: 121 FDNHVEFLITDFLSGPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQE 180

Query: 181 LIALLPISKYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINK 240
           LIALLPISKYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINK
Sbjct: 181 LIALLPISKYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINK 240

Query: 241 ACPICYTEVFGDTSKR 249
           ACPICYTEVFGDTSKR
Sbjct: 241 ACPICYTEVFGDTSKR 255

BLAST of CSPI01G30250 vs. TAIR 10
Match: AT3G63530.1 (RING/U-box superfamily protein )

HSP 1 Score: 218.8 bits (556), Expect = 5.0e-57
Identity = 114/252 (45.24%), Postives = 160/252 (63.49%), Query Frame = 0

Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           M+ ++ P  + H+  T +PY   GS+++++ G     +N+  +   H Q+N+Y  +N+N 
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPLNYDHAATMHPQDNLYWTMNTNA 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATHPNLQSTVNPE-----FEEI 120
           YKFGFS   + S+Y    +Y M+DH   +   R   N   HP +    +PE       +I
Sbjct: 61  YKFGFSGSDNASFYG---SYDMNDHLSRMSIGR--TNWDYHPMVNVADDPENTVARSVQI 120

Query: 121 TNTIAFDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIA 180
            +T       EC    H+    QV WQD++DPD MTYEEL+ELGE VGT+SRGLSQELI 
Sbjct: 121 GDTDEHSEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIE 180

Query: 181 LLPISKYKY-SFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKAC 240
            LP  KYK+ S FSRK++ GERCVICQ++YK G+R++ LPCKH+YH+ C +KWLSINK C
Sbjct: 181 TLPTKKYKFGSIFSRKRA-GERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVC 240

Query: 241 PICYTEVFGDTS 247
           P+C +EVFG+ S
Sbjct: 241 PVCNSEVFGEPS 246

BLAST of CSPI01G30250 vs. TAIR 10
Match: AT3G63530.2 (RING/U-box superfamily protein )

HSP 1 Score: 218.8 bits (556), Expect = 5.0e-57
Identity = 114/252 (45.24%), Postives = 160/252 (63.49%), Query Frame = 0

Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           M+ ++ P  + H+  T +PY   GS+++++ G     +N+  +   H Q+N+Y  +N+N 
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPLNYDHAATMHPQDNLYWTMNTNA 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATHPNLQSTVNPE-----FEEI 120
           YKFGFS   + S+Y    +Y M+DH   +   R   N   HP +    +PE       +I
Sbjct: 61  YKFGFSGSDNASFYG---SYDMNDHLSRMSIGR--TNWDYHPMVNVADDPENTVARSVQI 120

Query: 121 TNTIAFDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIA 180
            +T       EC    H+    QV WQD++DPD MTYEEL+ELGE VGT+SRGLSQELI 
Sbjct: 121 GDTDEHSEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIE 180

Query: 181 LLPISKYKY-SFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKAC 240
            LP  KYK+ S FSRK++ GERCVICQ++YK G+R++ LPCKH+YH+ C +KWLSINK C
Sbjct: 181 TLPTKKYKFGSIFSRKRA-GERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVC 240

Query: 241 PICYTEVFGDTS 247
           P+C +EVFG+ S
Sbjct: 241 PVCNSEVFGEPS 246

BLAST of CSPI01G30250 vs. TAIR 10
Match: AT3G19910.1 (RING/U-box superfamily protein )

HSP 1 Score: 118.2 bits (295), Expect = 9.2e-27
Identity = 56/113 (49.56%), Postives = 71/113 (62.83%), Query Frame = 0

Query: 134 SHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPISKYKYSFFSRKKSR 193
           SH  Q  W D +DPD ++YEELL LG+ VGT+SRGLS + IA LP  +YK      +   
Sbjct: 228 SHTSQDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEG--DNQNGT 287

Query: 194 GERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTEVFGDTS 247
            E CVIC+++Y+  +  I LPCKH YH+ C   WL INK CP+C  EV   TS
Sbjct: 288 NESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEVSTSTS 337

BLAST of CSPI01G30250 vs. TAIR 10
Match: AT5G52140.1 (RING/U-box superfamily protein )

HSP 1 Score: 107.1 bits (266), Expect = 2.1e-23
Identity = 57/119 (47.90%), Postives = 71/119 (59.66%), Query Frame = 0

Query: 132 HNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPISKYK------YS 191
           HN  D      +N DP+NMTYEEL ELG++VG   +GLSQE I+ L   KY       Y 
Sbjct: 166 HNPTD-----DENNDPENMTYEELTELGDSVGDVGKGLSQERISRLRTHKYGTKTKSWYC 225

Query: 192 FFSRKK--SRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTEVF 243
              +KK  +   +C IC MEY +GD+  TLPCKHIYH  C ++WL  NK C IC  EV+
Sbjct: 226 LHMKKKFVADDSQCSICLMEYAKGDKITTLPCKHIYHKDCISQWLKQNKVCCICKAEVY 279

BLAST of CSPI01G30250 vs. TAIR 10
Match: AT3G47180.1 (RING/U-box superfamily protein )

HSP 1 Score: 96.7 bits (239), Expect = 2.9e-20
Identity = 45/101 (44.55%), Postives = 65/101 (64.36%), Query Frame = 0

Query: 142 QDNVDPDNMTYEELLELGETVGTQSRGLSQ-ELIALLPISKYKYSFFSRKKSRGERCVIC 201
           +D +DPD ++YEEL+ LG+ +G ++RGL+  E+   L  S Y    FS  K+  +RCV+C
Sbjct: 109 EDEIDPDQLSYEELIALGDFIGVENRGLTPIEISTCLNASTY---VFSHNKNEIDRCVVC 168

Query: 202 QMEYKRGDRRITL-PCKHIYHAGCGTKWLSINKACPICYTE 241
           QME++  +  + L PC H YH+ C TKWL   K CPIC +E
Sbjct: 169 QMEFEERESLVVLRPCDHPYHSECITKWLETKKICPICCSE 206

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L6497.0e-5645.24E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE... [more]
Q9LT171.3e-2549.56E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR P... [more]
Q9ZQF91.6e-1545.00E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV... [more]
O495003.5e-1543.75E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV... [more]
Q7XTV77.9e-1545.74Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399... [more]
Match NameE-valueIdentityDescription
A0A0A0LX982.6e-14699.60RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G613470 P... [more]
A0A1S3BQ883.8e-14598.39E3 ubiquitin ligase BIG BROTHER-like OS=Cucumis melo OX=3656 GN=LOC103492041 PE=... [more]
A0A6J1JIX42.3e-13491.94E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita maxima OX=3661 GN=LOC111485509... [more]
A0A6J1HC342.3e-13491.13E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita moschata OX=3662 GN=LOC1114614... [more]
A0A5A7UF613.9e-13498.28E3 ubiquitin ligase BIG BROTHER-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
XP_011660125.15.4e-14699.60E3 ubiquitin-protein ligase BIG BROTHER [Cucumis sativus] >XP_011660126.1 E3 ubi... [more]
XP_008450441.17.8e-14598.39PREDICTED: E3 ubiquitin ligase BIG BROTHER-like [Cucumis melo] >XP_008450442.1 P... [more]
XP_038880080.11.8e-14197.18E3 ubiquitin-protein ligase BIG BROTHER-like isoform X2 [Benincasa hispida][more]
XP_038880079.15.4e-13893.75E3 ubiquitin-protein ligase BIG BROTHER-like isoform X1 [Benincasa hispida][more]
XP_038880082.15.4e-13893.75E3 ubiquitin-protein ligase BIG BROTHER-like isoform X3 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G63530.15.0e-5745.24RING/U-box superfamily protein [more]
AT3G63530.25.0e-5745.24RING/U-box superfamily protein [more]
AT3G19910.19.2e-2749.56RING/U-box superfamily protein [more]
AT5G52140.12.1e-2347.90RING/U-box superfamily protein [more]
AT3G47180.12.9e-2044.55RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 197..237
e-value: 2.8E-5
score: 33.5
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 196..237
e-value: 3.7E-11
score: 43.2
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 197..237
score: 10.851695
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 159..242
e-value: 2.2E-17
score: 64.5
IPR033276E3 ubiquitin-protein ligase BIG BROTHERPANTHERPTHR46400RING/U-BOX SUPERFAMILY PROTEINcoord: 1..247
NoneNo IPR availablePANTHERPTHR46400:SF5RING/U-BOX SUPERFAMILY PROTEINcoord: 1..247
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 196..242

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G30250.1CSPI01G30250.1mRNA
CSPI01G30250.2CSPI01G30250.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046621 negative regulation of organ growth
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0031624 ubiquitin conjugating enzyme binding
molecular_function GO:0004842 ubiquitin-protein transferase activity