CSPI01G30140 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G30140
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Pentatricopeptide repeat-containing protein
Location	Chr1: 24652007 .. 24652813 (+)
RNA-Seq Expression	CSPI01G30140
Synteny	CSPI01G30140

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGATCTCTAAGCTATAGTAAGTCTGTTTCCATATACAAAATTATCAGGCACATGGCCGCCGTCAAGGTGTGGCCGGATTGATCGATATGTATGCAAAATGCATGGCGGCTCTCAATGTGATATTCTTCAAGCTTCTTCACTAAGTCACTGTCTTCAGAAAAATGGTACGATGCGACTTTTTACTCAGTTCCTGCAAATCATTTCGCCAAATCAAACAAGTTCACGCCCGATTGATCACCACCGGCCTTATTCTACACCCAATCCCCACTAATAAACTCCTTAAACAACTTTCCTCAATATTCGCTCCAATTTCCTATGCCCACATGGTGTTCGACCATTTTCCTCAACCAGATCTCTTCCTCTACAACACCATTATCAAGGTCCTGGCATTTTCAACCACTTCTTCTGCTGATTCTTTCACCAAGTTTCGTTCTTTAATCCGCGACGAAAGGTTAGTGCCCAATCAGTATTCGTTTGCATTTGCCTTCAAGGGCTGTGGCAGTGGTGTTGGGGTTCTGGAAGGGGAGCAAGTTCGTGTTCATGCTATTAAACTTGGTCTGGAAAACAATCTGTTTGTGACGAATGCGTTGATTGGGATGTATGTGAATTTGGATTTTGTTGTGGATGCTAGAAAGGTGTTTGATTGGAGTCCCAATAGAGATATGTATTCCTGGAATATCATGCTTAGTGGGTATGCGAGATTGGGGAAAATGGATGAAGCTCGGCAACTGTTTGATGAAATGCCTGAAAAAGATGTTGTGTCGTGGACAACAATGATTTCTGGTTGTCTTCAGGTAATTACATGA

mRNA sequence

GTGATCTCTAAGCTATAGTAAGTCTGTTTCCATATACAAAATTATCAGGCACATGGCCGCCGTCAAGGTGTGGCCGGATTGATCGATATGTATGCAAAATGCATGGCGGCTCTCAATGTGATATTCTTCAAGCTTCTTCACTAAGTCACTGTCTTCAGAAAAATGGTACGATGCGACTTTTTACTCAGTTCCTGCAAATCATTTCGCCAAATCAAACAAGTTCACGCCCGATTGATCACCACCGGCCTTATTCTACACCCAATCCCCACTAATAAACTCCTTAAACAACTTTCCTCAATATTCGCTCCAATTTCCTATGCCCACATGGTGTTCGACCATTTTCCTCAACCAGATCTCTTCCTCTACAACACCATTATCAAGGTCCTGGCATTTTCAACCACTTCTTCTGCTGATTCTTTCACCAAGTTTCGTTCTTTAATCCGCGACGAAAGGTTAGTGCCCAATCAGTATTCGTTTGCATTTGCCTTCAAGGGCTGTGGCAGTGGTGTTGGGGTTCTGGAAGGGGAGCAAGTTCGTGTTCATGCTATTAAACTTGGTCTGGAAAACAATCTGTTTGTGACGAATGCGTTGATTGGGATGTATGTGAATTTGGATTTTGTTGTGGATGCTAGAAAGGTGTTTGATTGGAGTCCCAATAGAGATATGTATTCCTGGAATATCATGCTTAGTGGGTATGCGAGATTGGGGAAAATGGATGAAGCTCGGCAACTGTTTGATGAAATGCCTGAAAAAGATGTTGTGTCGTGGACAACAATGATTTCTGGTTGTCTTCAGGTAATTACATGA

Coding sequence (CDS)

ATGGTACGATGCGACTTTTTACTCAGTTCCTGCAAATCATTTCGCCAAATCAAACAAGTTCACGCCCGATTGATCACCACCGGCCTTATTCTACACCCAATCCCCACTAATAAACTCCTTAAACAACTTTCCTCAATATTCGCTCCAATTTCCTATGCCCACATGGTGTTCGACCATTTTCCTCAACCAGATCTCTTCCTCTACAACACCATTATCAAGGTCCTGGCATTTTCAACCACTTCTTCTGCTGATTCTTTCACCAAGTTTCGTTCTTTAATCCGCGACGAAAGGTTAGTGCCCAATCAGTATTCGTTTGCATTTGCCTTCAAGGGCTGTGGCAGTGGTGTTGGGGTTCTGGAAGGGGAGCAAGTTCGTGTTCATGCTATTAAACTTGGTCTGGAAAACAATCTGTTTGTGACGAATGCGTTGATTGGGATGTATGTGAATTTGGATTTTGTTGTGGATGCTAGAAAGGTGTTTGATTGGAGTCCCAATAGAGATATGTATTCCTGGAATATCATGCTTAGTGGGTATGCGAGATTGGGGAAAATGGATGAAGCTCGGCAACTGTTTGATGAAATGCCTGAAAAAGATGTTGTGTCGTGGACAACAATGATTTCTGGTTGTCTTCAGGTAATTACATGA

Protein sequence

MVRCDFLLSSCKSFRQIKQVHARLITTGLILHPIPTNKLLKQLSSIFAPISYAHMVFDHFPQPDLFLYNTIIKVLAFSTTSSADSFTKFRSLIRDERLVPNQYSFAFAFKGCGSGVGVLEGEQVRVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYARLGKMDEARQLFDEMPEKDVVSWTTMISGCLQVIT*

Homology

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BLAST of CSPI01G30140 vs. ExPASy Swiss-Prot
Match: Q9LN01 (Pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H12 PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 6.5e-34
Identity = 80/204 (39.22%), Postives = 119/204 (58.33%), Query Frame = 0


Query: 7   LLSSCKSFRQIKQVHARLITTGLILHPIPTNKLLK--QLSSIFAPISYAHMVFDHFPQPD 66
           LL +CK+ + ++ +HA++I  GL       +KL++   LS  F  + YA  VF    +P+
Sbjct: 39  LLHNCKTLQSLRIIHAQMIKIGLHNTNYALSKLIEFCILSPHFEGLPYAISVFKTIQEPN 98

Query: 67  LFLYNTIIKVLAFSTTSSADSFTKFRSLIRDERLVPNQYSFAFAFKGCGSGVGVLEGEQV 126
           L ++NT+ +  A S  S   S  K    +    L+PN Y+F F  K C       EG+Q+
Sbjct: 99  LLIWNTMFRGHALS--SDPVSALKLYVCMISLGLLPNSYTFPFVLKSCAKSKAFKEGQQI 158

Query: 127 RVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYARLGKM 186
             H +KLG + +L+V  +LI MYV    + DA KVFD SP+RD+ S+  ++ GYA  G +
Sbjct: 159 HGHVLKLGCDLDLYVHTSLISMYVQNGRLEDAHKVFDKSPHRDVVSYTALIKGYASRGYI 218

Query: 187 DEARQLFDEMPEKDVVSWTTMISG 209
           + A++LFDE+P KDVVSW  MISG
Sbjct: 219 ENAQKLFDEIPVKDVVSWNAMISG 240

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BLAST of CSPI01G30140 vs. ExPASy Swiss-Prot
Match: O49399 (Pentatricopeptide repeat-containing protein At4g18840 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E101 PE=3 SV=2)

HSP 1 Score: 139.4 bits (350), Expect = 4.7e-32
Identity = 78/199 (39.20%), Postives = 112/199 (56.28%), Query Frame = 0


Query: 12  KSFRQIKQVHARLITTGLILHPIPTNKLLKQLSSIFAP--ISYAHMVFDHFPQPDLFLYN 71
           KS  +I+Q HA ++ TGL       +KL+   ++   P  +SYAH + +    P+ F +N
Sbjct: 50  KSLTEIQQAHAFMLKTGLFHDTFSASKLVAFAATNPEPKTVSYAHSILNRIGSPNGFTHN 109

Query: 72  TIIKVLAFSTTSSADSFTKFRSLIRDERLVPNQYSFAFAFKGCGSGVGVLEGEQVRVHAI 131
           ++I+  A S+T    + T FR ++    + P++YSF F  K C +  G  EG Q+    I
Sbjct: 110 SVIRAYANSSTPEV-ALTVFREMLLGP-VFPDKYSFTFVLKACAAFCGFEEGRQIHGLFI 169

Query: 132 KLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYARLGKMDEARQ 191
           K GL  ++FV N L+ +Y    +   ARKV D  P RD  SWN +LS Y   G +DEAR 
Sbjct: 170 KSGLVTDVFVENTLVNVYGRSGYFEIARKVLDRMPVRDAVSWNSLLSAYLEKGLVDEARA 229

Query: 192 LFDEMPEKDVVSWTTMISG 209
           LFDEM E++V SW  MISG
Sbjct: 230 LFDEMEERNVESWNFMISG 246

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BLAST of CSPI01G30140 vs. ExPASy Swiss-Prot
Match: Q9SIL5 (Pentatricopeptide repeat-containing protein At2g20540 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E78 PE=2 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 1.8e-31
Identity = 77/201 (38.31%), Postives = 113/201 (56.22%), Query Frame = 0


Query: 8   LSSCKSFRQIKQVHARLITTGLILHPIPTNKLLKQLSSIFAPISYAHMVFDHFPQPDLFL 67
           L   KS  + K+++A +I  GL        K++     I   + YA  +F+    P++FL
Sbjct: 17  LQRVKSRNEWKKINASIIIHGLSQSSFMVTKMVDFCDKI-EDMDYATRLFNQVSNPNVFL 76

Query: 68  YNTIIKVLAFSTTSSADSFTKFRSLIRDERLVPNQYSFAFAFKGCGSGVGVLEGEQVRVH 127
           YN+II+    ++    D    ++ L+R    +P++++F F FK C S      G+QV  H
Sbjct: 77  YNSIIRAYTHNSL-YCDVIRIYKQLLRKSFELPDRFTFPFMFKSCASLGSCYLGKQVHGH 136

Query: 128 AIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYARLGKMDEA 187
             K G   ++   NALI MY+  D +VDA KVFD    RD+ SWN +LSGYARLG+M +A
Sbjct: 137 LCKFGPRFHVVTENALIDMYMKFDDLVDAHKVFDEMYERDVISWNSLLSGYARLGQMKKA 196

Query: 188 RQLFDEMPEKDVVSWTTMISG 209
           + LF  M +K +VSWT MISG
Sbjct: 197 KGLFHLMLDKTIVSWTAMISG 215

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BLAST of CSPI01G30140 vs. ExPASy Swiss-Prot
Match: Q9FJY7 (Pentatricopeptide repeat-containing protein At5g66520 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H61 PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.7e-29
Identity = 79/213 (37.09%), Postives = 111/213 (52.11%), Query Frame = 0


Query: 8   LSSCKSFRQIKQVHARLITTGLILHPIPTNKLL----KQLSSIFAPISYAHMVFDHFPQP 67
           L  C    ++KQ+HAR++ TGL+       K L       SS F P  YA +VFD F +P
Sbjct: 21  LQRCSKQEELKQIHARMLKTGLMQDSYAITKFLSFCISSTSSDFLP--YAQIVFDGFDRP 80

Query: 68  DLFLYNTIIKVLAFSTTSSADSFTKFRSLIRDERLV-----PNQYSFAFAFKGCGSGVGV 127
           D FL+N +I+   FS +   +     RSL+  +R++      N Y+F    K C +    
Sbjct: 81  DTFLWNLMIR--GFSCSDEPE-----RSLLLYQRMLCSSAPHNAYTFPSLLKACSNLSAF 140

Query: 128 LEGEQVRVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGY 187
            E  Q+     KLG EN+++  N+LI  Y        A  +FD  P  D  SWN ++ GY
Sbjct: 141 EETTQIHAQITKLGYENDVYAVNSLINSYAVTGNFKLAHLLFDRIPEPDDVSWNSVIKGY 200

Query: 188 ARLGKMDEARQLFDEMPEKDVVSWTTMISGCLQ 212
            + GKMD A  LF +M EK+ +SWTTMISG +Q
Sbjct: 201 VKAGKMDIALTLFRKMAEKNAISWTTMISGYVQ 224

[Close]

BLAST of CSPI01G30140 vs. ExPASy Swiss-Prot
Match: Q9FG16 (Pentatricopeptide repeat-containing protein At5g06540 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H88 PE=3 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 2.2e-29
Identity = 68/208 (32.69%), Postives = 113/208 (54.33%), Query Frame = 0


Query: 7   LLSSCKSFRQIKQVHARLITTGLILHPIPTNKLLKQL---SSIFAP---ISYAHMVFDHF 66
           LL SC SF  +K +H  L+ T LI      ++LL      S+   P   + YA+ +F   
Sbjct: 18  LLQSCSSFSDLKIIHGFLLRTHLISDVFVASRLLALCVDDSTFNKPTNLLGYAYGIFSQI 77

Query: 67  PQPDLFLYNTIIKVLAFSTTSSADSFTKFRSLIRDERLVPNQYSFAFAFKGCGSGVGVLE 126
             P+LF++N +I+   FST +       F + +   R+ P+  +F F  K       VL 
Sbjct: 78  QNPNLFVFNLLIR--CFSTGAEPSKAFGFYTQMLKSRIWPDNITFPFLIKASSEMECVLV 137

Query: 127 GEQVRVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYAR 186
           GEQ     ++ G +N+++V N+L+ MY N  F+  A ++F     RD+ SW  M++GY +
Sbjct: 138 GEQTHSQIVRFGFQNDVYVENSLVHMYANCGFIAAAGRIFGQMGFRDVVSWTSMVAGYCK 197

Query: 187 LGKMDEARQLFDEMPEKDVVSWTTMISG 209
            G ++ AR++FDEMP +++ +W+ MI+G
Sbjct: 198 CGMVENAREMFDEMPHRNLFTWSIMING 223

[Close]

BLAST of CSPI01G30140 vs. ExPASy TrEMBL
Match: A0A0A0LX83 (DYW_deaminase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G612890 PE=3 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 2.2e-117
Identity = 211/212 (99.53%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MVRCDFLLSSCKSFRQIKQVHARLITTGLILHPIPTNKLLKQLSSIFAPISYAHMVFDHF 60
           MVRCDFLLSSCKSFRQIKQVHARLITTGLILHPIPTNKLLKQLSSIFAPISYAHMVFDHF
Sbjct: 1   MVRCDFLLSSCKSFRQIKQVHARLITTGLILHPIPTNKLLKQLSSIFAPISYAHMVFDHF 60

Query: 61  PQPDLFLYNTIIKVLAFSTTSSADSFTKFRSLIRDERLVPNQYSFAFAFKGCGSGVGVLE 120
           PQPDLFLYNTIIKVLAFSTTSSADSFTKFRSLIR+ERLVPNQYSFAFAFKGCGSGVGVLE
Sbjct: 61  PQPDLFLYNTIIKVLAFSTTSSADSFTKFRSLIREERLVPNQYSFAFAFKGCGSGVGVLE 120

Query: 121 GEQVRVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYAR 180
           GEQVRVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYAR
Sbjct: 121 GEQVRVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYAR 180

Query: 181 LGKMDEARQLFDEMPEKDVVSWTTMISGCLQV 213
           LGKMDEARQLFDEMPEKDVVSWTTMISGCLQV
Sbjct: 181 LGKMDEARQLFDEMPEKDVVSWTTMISGCLQV 212

[Close]

BLAST of CSPI01G30140 vs. ExPASy TrEMBL
Match: A0A1S3BNM1 (pentatricopeptide repeat-containing protein At5g66520-like OS=Cucumis melo OX=3656 GN=LOC103492046 PE=3 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 5.6e-113
Identity = 202/212 (95.28%), Postives = 209/212 (98.58%), Query Frame = 0


Query: 1   MVRCDFLLSSCKSFRQIKQVHARLITTGLILHPIPTNKLLKQLSSIFAPISYAHMVFDHF 60
           MVRCDFLL SCKSFRQIKQVHA+LIT+GLILHPIPTNKLLKQLSSIFAPISYAHMVFDHF
Sbjct: 1   MVRCDFLLGSCKSFRQIKQVHAQLITSGLILHPIPTNKLLKQLSSIFAPISYAHMVFDHF 60

Query: 61  PQPDLFLYNTIIKVLAFSTTSSADSFTKFRSLIRDERLVPNQYSFAFAFKGCGSGVGVLE 120
           PQPDLFLYNTIIKVLAFSTTSSADSFT+FRSLIR+ERLVPNQYSFAFAFK CGSGVGVLE
Sbjct: 61  PQPDLFLYNTIIKVLAFSTTSSADSFTRFRSLIREERLVPNQYSFAFAFKACGSGVGVLE 120

Query: 121 GEQVRVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYAR 180
           GEQVRVHA+KLGLENNLFVTNALIGMYVNLDFVVDARKVF+WSP RDMYSWNIMLSGYAR
Sbjct: 121 GEQVRVHALKLGLENNLFVTNALIGMYVNLDFVVDARKVFEWSPYRDMYSWNIMLSGYAR 180

Query: 181 LGKMDEARQLFDEMPEKDVVSWTTMISGCLQV 213
           LGKMDEARQLFDEMPE+DVVSWTTMISGCLQV
Sbjct: 181 LGKMDEARQLFDEMPERDVVSWTTMISGCLQV 212

[Close]

BLAST of CSPI01G30140 vs. ExPASy TrEMBL
Match: A0A6J1HU41 (pentatricopeptide repeat-containing protein At5g66520-like OS=Cucurbita maxima OX=3661 GN=LOC111466592 PE=3 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 8.4e-93
Identity = 173/208 (83.17%), Postives = 184/208 (88.46%), Query Frame = 0


Query: 6   FLLSSCKSFRQIKQVHARLITTGLILHPIPTNKLLKQLS-SIFAPISYAHMVFDHFPQPD 65
           FLL  CKS  QIKQ HA+LITTGL+LHPI TNKLLK LS S F  ISYA MVFDHFPQPD
Sbjct: 12  FLLDFCKSIHQIKQTHAQLITTGLVLHPIATNKLLKLLSFSRFGSISYAQMVFDHFPQPD 71

Query: 66  LFLYNTIIKVLAFSTTSSADSFTKFRSLIRDERLVPNQYSFAFAFKGCGSGVGVLEGEQV 125
           LFLYNTIIK  A S TSSADSFT+FRSLIRD  LVPNQYSFAFAFKGCG+ VGVLEGEQV
Sbjct: 72  LFLYNTIIKAHAISATSSADSFTRFRSLIRDGSLVPNQYSFAFAFKGCGNAVGVLEGEQV 131

Query: 126 RVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYARLGKM 185
           RVHA+KLGLENNLFV NALIGMYVNL FV DARKVFDWS  RDMYSWNIMLSGYA+LGKM
Sbjct: 132 RVHAVKLGLENNLFVMNALIGMYVNLGFVGDARKVFDWSTIRDMYSWNIMLSGYAKLGKM 191

Query: 186 DEARQLFDEMPEKDVVSWTTMISGCLQV 213
           D+AR+LFDEMPE+DVVSWTTMI+GC+QV
Sbjct: 192 DDARELFDEMPERDVVSWTTMIAGCVQV 219

[Close]

BLAST of CSPI01G30140 vs. ExPASy TrEMBL
Match: A0A6J1EIF4 (pentatricopeptide repeat-containing protein At3g62890-like OS=Cucurbita moschata OX=3662 GN=LOC111434526 PE=3 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 1.1e-92
Identity = 173/208 (83.17%), Postives = 184/208 (88.46%), Query Frame = 0


Query: 6   FLLSSCKSFRQIKQVHARLITTGLILHPIPTNKLLKQLS-SIFAPISYAHMVFDHFPQPD 65
           FLL  CKS  QIKQ HA+LITTGL+LHPI TNKLLK LS S FA ISYA MVFDHFPQPD
Sbjct: 12  FLLDFCKSIHQIKQTHAQLITTGLVLHPIATNKLLKLLSFSRFASISYAQMVFDHFPQPD 71

Query: 66  LFLYNTIIKVLAFSTTSSADSFTKFRSLIRDERLVPNQYSFAFAFKGCGSGVGVLEGEQV 125
           LFLYNTIIK  A S TSSADSFT+FRSLIRD RLVPNQYSFAFAFKGCG+ VGVLEGEQV
Sbjct: 72  LFLYNTIIKAHALSATSSADSFTRFRSLIRDGRLVPNQYSFAFAFKGCGNAVGVLEGEQV 131

Query: 126 RVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYARLGKM 185
           R HA+KLGLENNLFV NALIGMYVNL  V DARKVFDWS  RDMYSWNIMLSGYA+LGKM
Sbjct: 132 RAHAVKLGLENNLFVMNALIGMYVNLGVVGDARKVFDWSTIRDMYSWNIMLSGYAKLGKM 191

Query: 186 DEARQLFDEMPEKDVVSWTTMISGCLQV 213
           D+AR+LFDEMPE+DVVSWTTMI+GC+QV
Sbjct: 192 DDARELFDEMPERDVVSWTTMIAGCVQV 219

[Close]

BLAST of CSPI01G30140 vs. ExPASy TrEMBL
Match: A0A5A7UA76 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold773G00170 PE=3 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 2.3e-82
Identity = 151/158 (95.57%), Postives = 156/158 (98.73%), Query Frame = 0


Query: 55  MVFDHFPQPDLFLYNTIIKVLAFSTTSSADSFTKFRSLIRDERLVPNQYSFAFAFKGCGS 114
           MVFDHFPQPDLFLYNTIIKVLAFSTTSSADSFT+FRSLIR+ERLVPNQYSFAFAFK CGS
Sbjct: 1   MVFDHFPQPDLFLYNTIIKVLAFSTTSSADSFTRFRSLIREERLVPNQYSFAFAFKACGS 60

Query: 115 GVGVLEGEQVRVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIM 174
           GVGVLEGEQVRVHA+KLGLENNLFVTNALIGMYVNLDFVVDARKVF+WSP RDMYSWNIM
Sbjct: 61  GVGVLEGEQVRVHALKLGLENNLFVTNALIGMYVNLDFVVDARKVFEWSPYRDMYSWNIM 120

Query: 175 LSGYARLGKMDEARQLFDEMPEKDVVSWTTMISGCLQV 213
           LSGYARLGKMDEARQLFDEMPE+DVVSWTTMISGCLQV
Sbjct: 121 LSGYARLGKMDEARQLFDEMPERDVVSWTTMISGCLQV 158

[Close]

BLAST of CSPI01G30140 vs. NCBI nr
Match: XP_004139110.1 (pentatricopeptide repeat-containing protein At5g66520 [Cucumis sativus] >KGN66465.1 hypothetical protein Csa_007004 [Cucumis sativus])

HSP 1 Score: 431.4 bits (1108), Expect = 4.5e-117
Identity = 211/212 (99.53%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MVRCDFLLSSCKSFRQIKQVHARLITTGLILHPIPTNKLLKQLSSIFAPISYAHMVFDHF 60
           MVRCDFLLSSCKSFRQIKQVHARLITTGLILHPIPTNKLLKQLSSIFAPISYAHMVFDHF
Sbjct: 1   MVRCDFLLSSCKSFRQIKQVHARLITTGLILHPIPTNKLLKQLSSIFAPISYAHMVFDHF 60

Query: 61  PQPDLFLYNTIIKVLAFSTTSSADSFTKFRSLIRDERLVPNQYSFAFAFKGCGSGVGVLE 120
           PQPDLFLYNTIIKVLAFSTTSSADSFTKFRSLIR+ERLVPNQYSFAFAFKGCGSGVGVLE
Sbjct: 61  PQPDLFLYNTIIKVLAFSTTSSADSFTKFRSLIREERLVPNQYSFAFAFKGCGSGVGVLE 120

Query: 121 GEQVRVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYAR 180
           GEQVRVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYAR
Sbjct: 121 GEQVRVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYAR 180

Query: 181 LGKMDEARQLFDEMPEKDVVSWTTMISGCLQV 213
           LGKMDEARQLFDEMPEKDVVSWTTMISGCLQV
Sbjct: 181 LGKMDEARQLFDEMPEKDVVSWTTMISGCLQV 212

[Close]

BLAST of CSPI01G30140 vs. NCBI nr
Match: XP_008450449.1 (PREDICTED: pentatricopeptide repeat-containing protein At5g66520-like [Cucumis melo])

HSP 1 Score: 416.8 bits (1070), Expect = 1.2e-112
Identity = 202/212 (95.28%), Postives = 209/212 (98.58%), Query Frame = 0


Query: 1   MVRCDFLLSSCKSFRQIKQVHARLITTGLILHPIPTNKLLKQLSSIFAPISYAHMVFDHF 60
           MVRCDFLL SCKSFRQIKQVHA+LIT+GLILHPIPTNKLLKQLSSIFAPISYAHMVFDHF
Sbjct: 1   MVRCDFLLGSCKSFRQIKQVHAQLITSGLILHPIPTNKLLKQLSSIFAPISYAHMVFDHF 60

Query: 61  PQPDLFLYNTIIKVLAFSTTSSADSFTKFRSLIRDERLVPNQYSFAFAFKGCGSGVGVLE 120
           PQPDLFLYNTIIKVLAFSTTSSADSFT+FRSLIR+ERLVPNQYSFAFAFK CGSGVGVLE
Sbjct: 61  PQPDLFLYNTIIKVLAFSTTSSADSFTRFRSLIREERLVPNQYSFAFAFKACGSGVGVLE 120

Query: 121 GEQVRVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYAR 180
           GEQVRVHA+KLGLENNLFVTNALIGMYVNLDFVVDARKVF+WSP RDMYSWNIMLSGYAR
Sbjct: 121 GEQVRVHALKLGLENNLFVTNALIGMYVNLDFVVDARKVFEWSPYRDMYSWNIMLSGYAR 180

Query: 181 LGKMDEARQLFDEMPEKDVVSWTTMISGCLQV 213
           LGKMDEARQLFDEMPE+DVVSWTTMISGCLQV
Sbjct: 181 LGKMDEARQLFDEMPERDVVSWTTMISGCLQV 212

[Close]

BLAST of CSPI01G30140 vs. NCBI nr
Match: XP_038878435.1 (pentatricopeptide repeat-containing protein At5g66520-like [Benincasa hispida])

HSP 1 Score: 388.3 bits (996), Expect = 4.4e-104
Identity = 192/219 (87.67%), Postives = 201/219 (91.78%), Query Frame = 0


Query: 1   MVRCD------FLLSSCKSFRQIKQVHARLITTGLILHPIPTNKLLKQLSSI-FAPISYA 60
           M+RCD      FLL SCKS  QIKQVHA+LITTGLI+HPIPTNKLLK +SS  FAPISYA
Sbjct: 1   MIRCDFTSKLFFLLDSCKSIHQIKQVHAQLITTGLIVHPIPTNKLLKLISSSRFAPISYA 60

Query: 61  HMVFDHFPQPDLFLYNTIIKVLAFSTTSSADSFTKFRSLIRDERLVPNQYSFAFAFKGCG 120
           HMVFDH PQPDLFLYNTIIK LA STTSSADSFT+FRSLIR+ERLVPNQYSFAF FKGCG
Sbjct: 61  HMVFDHCPQPDLFLYNTIIKALALSTTSSADSFTRFRSLIREERLVPNQYSFAFVFKGCG 120

Query: 121 SGVGVLEGEQVRVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNI 180
           +GVGVLEGEQVRVHA+KLGLENNLFV NALIGMYVNL FVVDARKVFDWSPNRDMYSWNI
Sbjct: 121 NGVGVLEGEQVRVHAVKLGLENNLFVMNALIGMYVNLGFVVDARKVFDWSPNRDMYSWNI 180

Query: 181 MLSGYARLGKMDEARQLFDEMPEKDVVSWTTMISGCLQV 213
           MLSGYARLGKMDEARQLFDEMPE+DVVSWTTMISGCLQV
Sbjct: 181 MLSGYARLGKMDEARQLFDEMPERDVVSWTTMISGCLQV 219

[Close]

BLAST of CSPI01G30140 vs. NCBI nr
Match: XP_023529533.1 (pentatricopeptide repeat-containing protein At3g62890-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 350.1 bits (897), Expect = 1.3e-92
Identity = 174/208 (83.65%), Postives = 185/208 (88.94%), Query Frame = 0


Query: 6   FLLSSCKSFRQIKQVHARLITTGLILHPIPTNKLLKQLS-SIFAPISYAHMVFDHFPQPD 65
           FLL  CKS  QIKQ HA+LITTGL+LHPI TNKLLK LS S FA ISYA MVFDHFPQPD
Sbjct: 12  FLLDFCKSIHQIKQTHAQLITTGLVLHPIATNKLLKLLSFSRFASISYAQMVFDHFPQPD 71

Query: 66  LFLYNTIIKVLAFSTTSSADSFTKFRSLIRDERLVPNQYSFAFAFKGCGSGVGVLEGEQV 125
           LFLYNTIIK  A S TSSADSFT+FRSLIRD RLVPNQYSFAFAFKGCG+ VGVLEGEQV
Sbjct: 72  LFLYNTIIKAHAISATSSADSFTRFRSLIRDGRLVPNQYSFAFAFKGCGNAVGVLEGEQV 131

Query: 126 RVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYARLGKM 185
           RVHA+KLGLENNLFV NALIGMYVNL FV  ARKVFDWS  RDMYSWNIMLSGYA+LGKM
Sbjct: 132 RVHAVKLGLENNLFVMNALIGMYVNLGFVGYARKVFDWSTIRDMYSWNIMLSGYAKLGKM 191

Query: 186 DEARQLFDEMPEKDVVSWTTMISGCLQV 213
           D+AR+LFDEMPE+DVVSWTTMI+GC+QV
Sbjct: 192 DDARELFDEMPERDVVSWTTMIAGCVQV 219

[Close]

BLAST of CSPI01G30140 vs. NCBI nr
Match: XP_022967088.1 (pentatricopeptide repeat-containing protein At5g66520-like [Cucurbita maxima])

HSP 1 Score: 349.7 bits (896), Expect = 1.7e-92
Identity = 173/208 (83.17%), Postives = 184/208 (88.46%), Query Frame = 0


Query: 6   FLLSSCKSFRQIKQVHARLITTGLILHPIPTNKLLKQLS-SIFAPISYAHMVFDHFPQPD 65
           FLL  CKS  QIKQ HA+LITTGL+LHPI TNKLLK LS S F  ISYA MVFDHFPQPD
Sbjct: 12  FLLDFCKSIHQIKQTHAQLITTGLVLHPIATNKLLKLLSFSRFGSISYAQMVFDHFPQPD 71

Query: 66  LFLYNTIIKVLAFSTTSSADSFTKFRSLIRDERLVPNQYSFAFAFKGCGSGVGVLEGEQV 125
           LFLYNTIIK  A S TSSADSFT+FRSLIRD  LVPNQYSFAFAFKGCG+ VGVLEGEQV
Sbjct: 72  LFLYNTIIKAHAISATSSADSFTRFRSLIRDGSLVPNQYSFAFAFKGCGNAVGVLEGEQV 131

Query: 126 RVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYARLGKM 185
           RVHA+KLGLENNLFV NALIGMYVNL FV DARKVFDWS  RDMYSWNIMLSGYA+LGKM
Sbjct: 132 RVHAVKLGLENNLFVMNALIGMYVNLGFVGDARKVFDWSTIRDMYSWNIMLSGYAKLGKM 191

Query: 186 DEARQLFDEMPEKDVVSWTTMISGCLQV 213
           D+AR+LFDEMPE+DVVSWTTMI+GC+QV
Sbjct: 192 DDARELFDEMPERDVVSWTTMIAGCVQV 219

[Close]

BLAST of CSPI01G30140 vs. TAIR 10
Match: AT1G08070.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 145.6 bits (366), Expect = 4.6e-35
Identity = 80/204 (39.22%), Postives = 119/204 (58.33%), Query Frame = 0


Query: 7   LLSSCKSFRQIKQVHARLITTGLILHPIPTNKLLK--QLSSIFAPISYAHMVFDHFPQPD 66
           LL +CK+ + ++ +HA++I  GL       +KL++   LS  F  + YA  VF    +P+
Sbjct: 39  LLHNCKTLQSLRIIHAQMIKIGLHNTNYALSKLIEFCILSPHFEGLPYAISVFKTIQEPN 98

Query: 67  LFLYNTIIKVLAFSTTSSADSFTKFRSLIRDERLVPNQYSFAFAFKGCGSGVGVLEGEQV 126
           L ++NT+ +  A S  S   S  K    +    L+PN Y+F F  K C       EG+Q+
Sbjct: 99  LLIWNTMFRGHALS--SDPVSALKLYVCMISLGLLPNSYTFPFVLKSCAKSKAFKEGQQI 158

Query: 127 RVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYARLGKM 186
             H +KLG + +L+V  +LI MYV    + DA KVFD SP+RD+ S+  ++ GYA  G +
Sbjct: 159 HGHVLKLGCDLDLYVHTSLISMYVQNGRLEDAHKVFDKSPHRDVVSYTALIKGYASRGYI 218

Query: 187 DEARQLFDEMPEKDVVSWTTMISG 209
           + A++LFDE+P KDVVSW  MISG
Sbjct: 219 ENAQKLFDEIPVKDVVSWNAMISG 240

[Close]

BLAST of CSPI01G30140 vs. TAIR 10
Match: AT4G18840.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 139.4 bits (350), Expect = 3.3e-33
Identity = 78/199 (39.20%), Postives = 112/199 (56.28%), Query Frame = 0


Query: 12  KSFRQIKQVHARLITTGLILHPIPTNKLLKQLSSIFAP--ISYAHMVFDHFPQPDLFLYN 71
           KS  +I+Q HA ++ TGL       +KL+   ++   P  +SYAH + +    P+ F +N
Sbjct: 50  KSLTEIQQAHAFMLKTGLFHDTFSASKLVAFAATNPEPKTVSYAHSILNRIGSPNGFTHN 109

Query: 72  TIIKVLAFSTTSSADSFTKFRSLIRDERLVPNQYSFAFAFKGCGSGVGVLEGEQVRVHAI 131
           ++I+  A S+T    + T FR ++    + P++YSF F  K C +  G  EG Q+    I
Sbjct: 110 SVIRAYANSSTPEV-ALTVFREMLLGP-VFPDKYSFTFVLKACAAFCGFEEGRQIHGLFI 169

Query: 132 KLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYARLGKMDEARQ 191
           K GL  ++FV N L+ +Y    +   ARKV D  P RD  SWN +LS Y   G +DEAR 
Sbjct: 170 KSGLVTDVFVENTLVNVYGRSGYFEIARKVLDRMPVRDAVSWNSLLSAYLEKGLVDEARA 229

Query: 192 LFDEMPEKDVVSWTTMISG 209
           LFDEM E++V SW  MISG
Sbjct: 230 LFDEMEERNVESWNFMISG 246

[Close]

BLAST of CSPI01G30140 vs. TAIR 10
Match: AT2G20540.1 (mitochondrial editing factor 21 )

HSP 1 Score: 137.5 bits (345), Expect = 1.3e-32
Identity = 77/201 (38.31%), Postives = 113/201 (56.22%), Query Frame = 0


Query: 8   LSSCKSFRQIKQVHARLITTGLILHPIPTNKLLKQLSSIFAPISYAHMVFDHFPQPDLFL 67
           L   KS  + K+++A +I  GL        K++     I   + YA  +F+    P++FL
Sbjct: 17  LQRVKSRNEWKKINASIIIHGLSQSSFMVTKMVDFCDKI-EDMDYATRLFNQVSNPNVFL 76

Query: 68  YNTIIKVLAFSTTSSADSFTKFRSLIRDERLVPNQYSFAFAFKGCGSGVGVLEGEQVRVH 127
           YN+II+    ++    D    ++ L+R    +P++++F F FK C S      G+QV  H
Sbjct: 77  YNSIIRAYTHNSL-YCDVIRIYKQLLRKSFELPDRFTFPFMFKSCASLGSCYLGKQVHGH 136

Query: 128 AIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYARLGKMDEA 187
             K G   ++   NALI MY+  D +VDA KVFD    RD+ SWN +LSGYARLG+M +A
Sbjct: 137 LCKFGPRFHVVTENALIDMYMKFDDLVDAHKVFDEMYERDVISWNSLLSGYARLGQMKKA 196

Query: 188 RQLFDEMPEKDVVSWTTMISG 209
           + LF  M +K +VSWT MISG
Sbjct: 197 KGLFHLMLDKTIVSWTAMISG 215

[Close]

BLAST of CSPI01G30140 vs. TAIR 10
Match: AT5G66520.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 131.0 bits (328), Expect = 1.2e-30
Identity = 79/213 (37.09%), Postives = 111/213 (52.11%), Query Frame = 0


Query: 8   LSSCKSFRQIKQVHARLITTGLILHPIPTNKLL----KQLSSIFAPISYAHMVFDHFPQP 67
           L  C    ++KQ+HAR++ TGL+       K L       SS F P  YA +VFD F +P
Sbjct: 21  LQRCSKQEELKQIHARMLKTGLMQDSYAITKFLSFCISSTSSDFLP--YAQIVFDGFDRP 80

Query: 68  DLFLYNTIIKVLAFSTTSSADSFTKFRSLIRDERLV-----PNQYSFAFAFKGCGSGVGV 127
           D FL+N +I+   FS +   +     RSL+  +R++      N Y+F    K C +    
Sbjct: 81  DTFLWNLMIR--GFSCSDEPE-----RSLLLYQRMLCSSAPHNAYTFPSLLKACSNLSAF 140

Query: 128 LEGEQVRVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGY 187
            E  Q+     KLG EN+++  N+LI  Y        A  +FD  P  D  SWN ++ GY
Sbjct: 141 EETTQIHAQITKLGYENDVYAVNSLINSYAVTGNFKLAHLLFDRIPEPDDVSWNSVIKGY 200

Query: 188 ARLGKMDEARQLFDEMPEKDVVSWTTMISGCLQ 212
            + GKMD A  LF +M EK+ +SWTTMISG +Q
Sbjct: 201 VKAGKMDIALTLFRKMAEKNAISWTTMISGYVQ 224

[Close]

BLAST of CSPI01G30140 vs. TAIR 10
Match: AT5G06540.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 130.6 bits (327), Expect = 1.5e-30
Identity = 68/208 (32.69%), Postives = 113/208 (54.33%), Query Frame = 0


Query: 7   LLSSCKSFRQIKQVHARLITTGLILHPIPTNKLLKQL---SSIFAP---ISYAHMVFDHF 66
           LL SC SF  +K +H  L+ T LI      ++LL      S+   P   + YA+ +F   
Sbjct: 18  LLQSCSSFSDLKIIHGFLLRTHLISDVFVASRLLALCVDDSTFNKPTNLLGYAYGIFSQI 77

Query: 67  PQPDLFLYNTIIKVLAFSTTSSADSFTKFRSLIRDERLVPNQYSFAFAFKGCGSGVGVLE 126
             P+LF++N +I+   FST +       F + +   R+ P+  +F F  K       VL 
Sbjct: 78  QNPNLFVFNLLIR--CFSTGAEPSKAFGFYTQMLKSRIWPDNITFPFLIKASSEMECVLV 137

Query: 127 GEQVRVHAIKLGLENNLFVTNALIGMYVNLDFVVDARKVFDWSPNRDMYSWNIMLSGYAR 186
           GEQ     ++ G +N+++V N+L+ MY N  F+  A ++F     RD+ SW  M++GY +
Sbjct: 138 GEQTHSQIVRFGFQNDVYVENSLVHMYANCGFIAAAGRIFGQMGFRDVVSWTSMVAGYCK 197

Query: 187 LGKMDEARQLFDEMPEKDVVSWTTMISG 209
            G ++ AR++FDEMP +++ +W+ MI+G
Sbjct: 198 CGMVENAREMFDEMPHRNLFTWSIMING 223

The following BLAST results are available for this feature:

BLAST of CSPI01G30140 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LN01	6.5e-34	39.22	Pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Arabidop...	[more]
O49399	4.7e-32	39.20	Pentatricopeptide repeat-containing protein At4g18840 OS=Arabidopsis thaliana OX...	[more]
Q9SIL5	1.8e-31	38.31	Pentatricopeptide repeat-containing protein At2g20540 OS=Arabidopsis thaliana OX...	[more]
Q9FJY7	1.7e-29	37.09	Pentatricopeptide repeat-containing protein At5g66520 OS=Arabidopsis thaliana OX...	[more]
Q9FG16	2.2e-29	32.69	Pentatricopeptide repeat-containing protein At5g06540 OS=Arabidopsis thaliana OX...	[more]

BLAST of CSPI01G30140 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LX83	2.2e-117	99.53	DYW_deaminase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G6128...	[more]
A0A1S3BNM1	5.6e-113	95.28	pentatricopeptide repeat-containing protein At5g66520-like OS=Cucumis melo OX=36...	[more]
A0A6J1HU41	8.4e-93	83.17	pentatricopeptide repeat-containing protein At5g66520-like OS=Cucurbita maxima O...	[more]
A0A6J1EIF4	1.1e-92	83.17	pentatricopeptide repeat-containing protein At3g62890-like OS=Cucurbita moschata...	[more]
A0A5A7UA76	2.3e-82	95.57	Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946...	[more]

BLAST of CSPI01G30140 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139110.1	4.5e-117	99.53	pentatricopeptide repeat-containing protein At5g66520 [Cucumis sativus] >KGN6646...	[more]
XP_008450449.1	1.2e-112	95.28	PREDICTED: pentatricopeptide repeat-containing protein At5g66520-like [Cucumis m...	[more]
XP_038878435.1	4.4e-104	87.67	pentatricopeptide repeat-containing protein At5g66520-like [Benincasa hispida]	[more]
XP_023529533.1	1.3e-92	83.65	pentatricopeptide repeat-containing protein At3g62890-like [Cucurbita pepo subsp...	[more]
XP_022967088.1	1.7e-92	83.17	pentatricopeptide repeat-containing protein At5g66520-like [Cucurbita maxima]	[more]

BLAST of CSPI01G30140 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G08070.1	4.6e-35	39.22	Tetratricopeptide repeat (TPR)-like superfamily protein	[more]
AT4G18840.1	3.3e-33	39.20	Pentatricopeptide repeat (PPR-like) superfamily protein	[more]
AT2G20540.1	1.3e-32	38.31	mitochondrial editing factor 21	[more]
AT5G66520.1	1.2e-30	37.09	Tetratricopeptide repeat (TPR)-like superfamily protein	[more]
AT5G06540.1	1.5e-30	32.69	Pentatricopeptide repeat (PPR) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011990	Tetratricopeptide-like helical domain superfamily	GENE3D	1.25.40.10	Tetratricopeptide repeat domain	coord: 78..212 e-value: 1.4E-22 score: 82.5
IPR002885	Pentatricopeptide repeat	PFAM	PF01535	PPR	coord: 169..198 e-value: 1.6E-10 score: 40.5
IPR002885	Pentatricopeptide repeat	TIGRFAM	TIGR00756	TIGR00756	coord: 169..200 e-value: 5.6E-10 score: 36.8
IPR002885	Pentatricopeptide repeat	PROSITE	PS51375	PPR	coord: 167..201 score: 13.394766
None	No IPR available	PANTHER	PTHR47928	REPEAT-CONTAINING PROTEIN, PUTATIVE-RELATED	coord: 11..212
None	No IPR available	PANTHER	PTHR47928:SF65	TRANSCRIPT PROCESSING PROTEIN, PUTATIVE-RELATED	coord: 11..212

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G30140.1	CSPI01G30140.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1900865	chloroplast RNA modification
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding