CSPI01G28370 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G28370
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionDDE Tnp4 domain-containing protein
LocationChr1: 23071239 .. 23073124 (+)
RNA-Seq ExpressionCSPI01G28370
SyntenyCSPI01G28370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCTCACTTCAATCTTCTCTTTTCACATTTGCTCAACACAAAGTTATTCATTCATACCCACTTCCATTTCATGGAAATCTCCTCTTTCCCATTTCTTAATCAAGAAGAATTCTTACCCATCTTCAATCTCTTCTCCGAAATGGATAATCCCACCGCCACTTTCAATGTGAATCCAACATCTAAAAAACGGCGAAGATCTGACCCAAATTCCGATGACTTCAACAGTTTTTCTTTCACTGATGAAAACGATGATCCTACTGCTGACCCACTACTAAAACTCCCCTGTTGGTTCGATCCCCAACCCGAATCTCAACAAAATTGGCTCATGGACGCCCAAAAACCAAAACCCACCAACGATTTCCATCTCTCCGATCAAATTCCCAAAAAACCCCGCCGTGCATCGCCGGAAAATCCCTCTCCGGTGAAGAATACCCCCGCCGGTGGTGGAGGAACTCAGCAGCGACGGCTATGGGTGAAAGACCGATCCAAGGATTGGTGGGATCAATGCAACCACCCGGATTTTCCCGATGAGGAGTTCCGACGAGCATTCCGGATGAGTAAATCCACATTCGATATGATCTGTAAAGAACTGGATTCAACGGTGATGAAAAAAGACACAATGCTTCGTGTTGCGATTCCCGTACGGCAGCGTGTTGCCGTCTGTATATGGCGGTTGGCTACCGGAGAGCCACTCCGATTAGTTTCGAAAAGATTTGGGTTAGGGATTTCAACTTGCCATAAATTAGTTCTGGAAGTTTGTTCAGCGATTCGTAAAGTTCTAATGCCGAAATTCCTCAATTGGCCAGATGAATCAAAATTAACCAAAATCAAACAAGAATTCGAATCGATTTCAGGAATTCCCAAAGTCGGCGGTTCAATTTACACAACACATATCCCAATAATCGCACCAAAAAACAACGTAGCTGCTTATTTCAACAAACGCCACACAGAACGCAACCAAAAAACTTCATACTCCATCACTGTTCAAGGCGTCGTCGATCCCTCCGGTGTATTCACCGACGTTTGCATCGGATGGCCGGGATCTATGCCGGACGATCAAGTTCTTGAGAAATCATTGCTTTACGAAAGAGCAAGTATGGGGTCATTGAACGATGTGTTCATCGTCGGAAATTCAGGGTACCCATTAATGGATTGGTTGTTAGTTCCTTATACTGTACAGAATTTGACATGGACACAACATGGGTTTAATGAGAAAGTTGGGGAGATTCAGGCGGCAGCGAAGGCGGCGTTTGGGCGGTTGAAAGGGCGATGGACTTGTTTACAGAAAAGAACAGAGGTGAAACTGCAGGAGTTGCCGGTGGTGCTGGGAGCTTGCTGTGTTCTTCATAATATATGTGAAATGAGGAAGGAGAAATTCGATCCGGAGCTGAAATTTGAGGTTTATGATGATGAAATGATGCCGGAAAACAACGGGTTGAGATCGGTGAGTGCGATTCAAGCTAGAGATCATATTGCTCATAATCTTCTTCACCATGGAATTGCTGGGACAGGATTTCTTTGATGAGATTGGATTTTGATGAACAATGAAGAATCTATATATAAATATGTATTTACATTTACAATTATTTGTTCAAAAAAACTAATAAAAGAAATATTAAGATTTGTTTATTTGAAAGTATACTTGTAATTTGGAGGGAAGATTTGGTAGCTTTTAGGGTGATTTTAGGGGTTAGTGGTAGTGTTTTACATATTGAATCAAGAGAGAAAGGGATGTAATTGAGCAATGTCTCTTTTTCAATCTTTTTTGAATGGAAGATTAGATTGCTTTTCTTTTATAATTTGGGAGTTAGATTTTGTTTTCTTGATCATGGAAAAAAATTGGATTGATAGGTACATTTGTGTAATGAAAAATTGAAGATGTGGA

mRNA sequence

ATTCTCACTTCAATCTTCTCTTTTCACATTTGCTCAACACAAAGTTATTCATTCATACCCACTTCCATTTCATGGAAATCTCCTCTTTCCCATTTCTTAATCAAGAAGAATTCTTACCCATCTTCAATCTCTTCTCCGAAATGGATAATCCCACCGCCACTTTCAATGTGAATCCAACATCTAAAAAACGGCGAAGATCTGACCCAAATTCCGATGACTTCAACAGTTTTTCTTTCACTGATGAAAACGATGATCCTACTGCTGACCCACTACTAAAACTCCCCTGTTGGTTCGATCCCCAACCCGAATCTCAACAAAATTGGCTCATGGACGCCCAAAAACCAAAACCCACCAACGATTTCCATCTCTCCGATCAAATTCCCAAAAAACCCCGCCGTGCATCGCCGGAAAATCCCTCTCCGGTGAAGAATACCCCCGCCGGTGGTGGAGGAACTCAGCAGCGACGGCTATGGGTGAAAGACCGATCCAAGGATTGGTGGGATCAATGCAACCACCCGGATTTTCCCGATGAGGAGTTCCGACGAGCATTCCGGATGAGTAAATCCACATTCGATATGATCTGTAAAGAACTGGATTCAACGGTGATGAAAAAAGACACAATGCTTCGTGTTGCGATTCCCGTACGGCAGCGTGTTGCCGTCTGTATATGGCGGTTGGCTACCGGAGAGCCACTCCGATTAGTTTCGAAAAGATTTGGGTTAGGGATTTCAACTTGCCATAAATTAGTTCTGGAAGTTTGTTCAGCGATTCGTAAAGTTCTAATGCCGAAATTCCTCAATTGGCCAGATGAATCAAAATTAACCAAAATCAAACAAGAATTCGAATCGATTTCAGGAATTCCCAAAGTCGGCGGTTCAATTTACACAACACATATCCCAATAATCGCACCAAAAAACAACGTAGCTGCTTATTTCAACAAACGCCACACAGAACGCAACCAAAAAACTTCATACTCCATCACTGTTCAAGGCGTCGTCGATCCCTCCGGTGTATTCACCGACGTTTGCATCGGATGGCCGGGATCTATGCCGGACGATCAAGTTCTTGAGAAATCATTGCTTTACGAAAGAGCAAGTATGGGGTCATTGAACGATGTGTTCATCGTCGGAAATTCAGGGTACCCATTAATGGATTGGTTGTTAGTTCCTTATACTGTACAGAATTTGACATGGACACAACATGGGTTTAATGAGAAAGTTGGGGAGATTCAGGCGGCAGCGAAGGCGGCGTTTGGGCGGTTGAAAGGGCGATGGACTTGTTTACAGAAAAGAACAGAGGTGAAACTGCAGGAGTTGCCGGTGGTGCTGGGAGCTTGCTGTGTTCTTCATAATATATGTGAAATGAGGAAGGAGAAATTCGATCCGGAGCTGAAATTTGAGGTTTATGATGATGAAATGATGCCGGAAAACAACGGGTTGAGATCGGTGAGTGCGATTCAAGCTAGAGATCATATTGCTCATAATCTTCTTCACCATGGAATTGCTGGGACAGGATTTCTTTGATGAGATTGGATTTTGATGAACAATGAAGAATCTATATATAAATATGTATTTACATTTACAATTATTTGTTCAAAAAAACTAATAAAAGAAATATTAAGATTTGTTTATTTGAAAGTATACTTGTAATTTGGAGGGAAGATTTGGTAGCTTTTAGGGTGATTTTAGGGGTTAGTGGTAGTGTTTTACATATTGAATCAAGAGAGAAAGGGATGTAATTGAGCAATGTCTCTTTTTCAATCTTTTTTGAATGGAAGATTAGATTGCTTTTCTTTTATAATTTGGGAGTTAGATTTTGTTTTCTTGATCATGGAAAAAAATTGGATTGATAGGTACATTTGTGTAATGAAAAATTGAAGATGTGGA

Coding sequence (CDS)

ATGGAAATCTCCTCTTTCCCATTTCTTAATCAAGAAGAATTCTTACCCATCTTCAATCTCTTCTCCGAAATGGATAATCCCACCGCCACTTTCAATGTGAATCCAACATCTAAAAAACGGCGAAGATCTGACCCAAATTCCGATGACTTCAACAGTTTTTCTTTCACTGATGAAAACGATGATCCTACTGCTGACCCACTACTAAAACTCCCCTGTTGGTTCGATCCCCAACCCGAATCTCAACAAAATTGGCTCATGGACGCCCAAAAACCAAAACCCACCAACGATTTCCATCTCTCCGATCAAATTCCCAAAAAACCCCGCCGTGCATCGCCGGAAAATCCCTCTCCGGTGAAGAATACCCCCGCCGGTGGTGGAGGAACTCAGCAGCGACGGCTATGGGTGAAAGACCGATCCAAGGATTGGTGGGATCAATGCAACCACCCGGATTTTCCCGATGAGGAGTTCCGACGAGCATTCCGGATGAGTAAATCCACATTCGATATGATCTGTAAAGAACTGGATTCAACGGTGATGAAAAAAGACACAATGCTTCGTGTTGCGATTCCCGTACGGCAGCGTGTTGCCGTCTGTATATGGCGGTTGGCTACCGGAGAGCCACTCCGATTAGTTTCGAAAAGATTTGGGTTAGGGATTTCAACTTGCCATAAATTAGTTCTGGAAGTTTGTTCAGCGATTCGTAAAGTTCTAATGCCGAAATTCCTCAATTGGCCAGATGAATCAAAATTAACCAAAATCAAACAAGAATTCGAATCGATTTCAGGAATTCCCAAAGTCGGCGGTTCAATTTACACAACACATATCCCAATAATCGCACCAAAAAACAACGTAGCTGCTTATTTCAACAAACGCCACACAGAACGCAACCAAAAAACTTCATACTCCATCACTGTTCAAGGCGTCGTCGATCCCTCCGGTGTATTCACCGACGTTTGCATCGGATGGCCGGGATCTATGCCGGACGATCAAGTTCTTGAGAAATCATTGCTTTACGAAAGAGCAAGTATGGGGTCATTGAACGATGTGTTCATCGTCGGAAATTCAGGGTACCCATTAATGGATTGGTTGTTAGTTCCTTATACTGTACAGAATTTGACATGGACACAACATGGGTTTAATGAGAAAGTTGGGGAGATTCAGGCGGCAGCGAAGGCGGCGTTTGGGCGGTTGAAAGGGCGATGGACTTGTTTACAGAAAAGAACAGAGGTGAAACTGCAGGAGTTGCCGGTGGTGCTGGGAGCTTGCTGTGTTCTTCATAATATATGTGAAATGAGGAAGGAGAAATTCGATCCGGAGCTGAAATTTGAGGTTTATGATGATGAAATGATGCCGGAAAACAACGGGTTGAGATCGGTGAGTGCGATTCAAGCTAGAGATCATATTGCTCATAATCTTCTTCACCATGGAATTGCTGGGACAGGATTTCTTTGA

Protein sequence

MEISSFPFLNQEEFLPIFNLFSEMDNPTATFNVNPTSKKRRRSDPNSDDFNSFSFTDENDDPTADPLLKLPCWFDPQPESQQNWLMDAQKPKPTNDFHLSDQIPKKPRRASPENPSPVKNTPAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLNWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGSLNDVFIVGNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICEMRKEKFDPELKFEVYDDEMMPENNGLRSVSAIQARDHIAHNLLHHGIAGTGFL*
Homology
BLAST of CSPI01G28370 vs. ExPASy Swiss-Prot
Match: Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 8.9e-39
Identity = 112/362 (30.94%), Postives = 176/362 (48.62%), Query Frame = 0

Query: 138 RSKDWWDQCNHPDF----PDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVA----I 197
           +S DWWD  +   +      + F   F++S+ TFD IC  + +    K      +    +
Sbjct: 50  QSLDWWDGFSRRIYGGSTDPKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPL 109

Query: 198 PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLNWPDESK 257
            +  RVAV + RL +GE L ++ + FG+  ST  ++      ++ +  +   L+WP  SK
Sbjct: 110 SLNDRVAVALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAI-HHLSWP--SK 169

Query: 258 LTKIKQEFESISGIPKVGGSIYTTHI----PIIAPKNNVAAYFNKRHTERNQKTSYSITV 317
           L +IK +FE ISG+P   G+I  THI    P + P N V           + + ++S+T+
Sbjct: 170 LDEIKSKFEKISGLPNCCGAIDITHIVMNLPAVEPSNKVWL---------DGEKNFSMTL 229

Query: 318 QGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGS-LND------------VFIV 377
           Q VVDP   F DV  GWPGS+ DD VL+ S  Y+    G  LN              +IV
Sbjct: 230 QAVVDPDMRFLDVIAGWPGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIV 289

Query: 378 GNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEV-K 437
           G+SG+PL+ WLL PY  +  +  Q  FN++  E   AA+ A  +LK RW  +     +  
Sbjct: 290 GDSGFPLLPWLLTPYQGKPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPD 349

Query: 438 LQELPVVLGACCVLHN-ICEMRKEKFDPELKFEVYDDEMMPENNGLRSVSAIQARDHIAH 473
              LP ++  CC+LHN I +M  +  D +   + +D      +  L   ++   RD ++ 
Sbjct: 350 RNRLPRIIFVCCLLHNIIIDMEDQTLDDQPLSQQHDMNYRQRSCKLADEASSVLRDELSD 399

BLAST of CSPI01G28370 vs. ExPASy Swiss-Prot
Match: Q94K49 (Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=ALP1 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.5e-33
Identity = 93/314 (29.62%), Postives = 156/314 (49.68%), Query Frame = 0

Query: 141 DWWD----QCNHPDFPDEE---FRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAI---- 200
           DWWD    + + P  P +E   F+  FR SK+TF  IC  +   ++ +     + I    
Sbjct: 43  DWWDTFWLRNSSPSVPSDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRL 102

Query: 201 -PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLNWPDES 260
             V ++VA+ + RLA+G+    V   FG+G ST  ++      A+ +      L WPD  
Sbjct: 103 LSVEKQVAIALRRLASGDSQVSVGAAFGVGQSTVSQVTWRFIEALEE-RAKHHLRWPDSD 162

Query: 261 KLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGV 320
           ++ +IK +FE + G+P   G+I TTHI +  P    +  +       +Q+ +YS+ +QGV
Sbjct: 163 RIEEIKSKFEEMYGLPNCCGAIDTTHIIMTLPAVQASDDW------CDQEKNYSMFLQGV 222

Query: 321 VDPSGVFTDVCIGWPGSMPDDQVLEKSLLY-------------ERASMGSLNDVFIVGNS 380
            D    F ++  GWPG M   ++L+ S  +             +  S G+    ++VG  
Sbjct: 223 FDHEMRFLNMVTGWPGGMTVSKLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGI 282

Query: 381 GYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQK-RTEVKLQE 429
            YPL+ WL+ P+   + + +   FNE+  ++++ A  AF +LKG W  L K       ++
Sbjct: 283 SYPLLPWLITPHDSDHPSDSMVAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRK 342

BLAST of CSPI01G28370 vs. ExPASy Swiss-Prot
Match: Q96MB7 (Putative nuclease HARBI1 OS=Homo sapiens OX=9606 GN=HARBI1 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 7.3e-17
Identity = 69/261 (26.44%), Postives = 117/261 (44.83%), Query Frame = 0

Query: 173 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 232
           EL    + + T    AI    +V   +    +G     +    G+  ++  + V  V  A
Sbjct: 51  ELLGANLSRPTQRSRAISPETQVLAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEA 110

Query: 233 IRKVLMPKFLNWP-DESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKR 292
           + +    +F+ +P DE+ +  +K EF  ++G+P V G +   H+ I AP     +Y N+ 
Sbjct: 111 LVE-RASQFIRFPADEASIQALKDEFYGLAGMPGVMGVVDCIHVAIKAPNAEDLSYVNR- 170

Query: 293 HTERNQKTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGSLNDVFI 352
                 K  +S+    V D  G    V   WPGS+ D  VL++S L  +   G   D ++
Sbjct: 171 ------KGLHSLNCLMVCDIRGTLMTVETNWPGSLQDCAVLQQSSLSSQFEAGMHKDSWL 230

Query: 353 VGNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVK 412
           +G+S + L  WL+ P  +   T  ++ +N       +  +  F  L  R+ CL   ++  
Sbjct: 231 LGDSSFFLRTWLMTPLHIPE-TPAEYRYNMAHSATHSVIEKTFRTLCSRFRCLD-GSKGA 290

Query: 413 LQELPV----VLGACCVLHNI 429
           LQ  P     ++ ACCVLHNI
Sbjct: 291 LQYSPEKSSHIILACCVLHNI 301

BLAST of CSPI01G28370 vs. ExPASy Swiss-Prot
Match: Q17QR8 (Putative nuclease HARBI1 OS=Bos taurus OX=9913 GN=HARBI1 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.6e-16
Identity = 68/261 (26.05%), Postives = 118/261 (45.21%), Query Frame = 0

Query: 173 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 232
           EL    + + T    AI    ++   +    +G     +    G+  ++  + V  V  A
Sbjct: 51  ELLGASLSRPTQRSRAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEA 110

Query: 233 IRKVLMPKFLNWP-DESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKR 292
           + +    +F+++P DE+ +  +K EF  ++GIP V G +   H+ I AP     +Y N+ 
Sbjct: 111 LVE-RASQFIHFPADEASVQALKDEFYGLAGIPGVIGVVDCMHVAIKAPNAEDLSYVNR- 170

Query: 293 HTERNQKTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGSLNDVFI 352
                 K  +S+    V D  G    V   WPGS+ D  VL++S L  +   G   + ++
Sbjct: 171 ------KGLHSLNCLMVCDIRGALMTVETSWPGSLQDCVVLQQSSLSSQFEAGMHKESWL 230

Query: 353 VGNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVK 412
           +G+S + L  WL+ P  +   T  ++ +N       +  +  F  L  R+ CL   ++  
Sbjct: 231 LGDSSFFLRTWLMTPLHIPE-TPAEYRYNMAHSATHSVIEKTFRTLCSRFRCLD-GSKGA 290

Query: 413 LQELPV----VLGACCVLHNI 429
           LQ  P     ++ ACCVLHNI
Sbjct: 291 LQYSPEKSSHIILACCVLHNI 301

BLAST of CSPI01G28370 vs. ExPASy Swiss-Prot
Match: B0BN95 (Putative nuclease HARBI1 OS=Rattus norvegicus OX=10116 GN=Harbi1 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.6e-16
Identity = 68/261 (26.05%), Postives = 118/261 (45.21%), Query Frame = 0

Query: 173 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 232
           EL    + + T    AI    ++   +    +G     +    G+  ++  + V  V  A
Sbjct: 51  ELLGASLSRPTQRSRAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEA 110

Query: 233 IRKVLMPKFLNWP-DESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKR 292
           + +    +F+++P DE+ +  +K EF  ++G+P V G++   H+ I AP     +Y N+ 
Sbjct: 111 LVE-RASQFIHFPADEAAIQSLKDEFYGLAGMPGVIGAVDCIHVAIKAPNAEDLSYVNR- 170

Query: 293 HTERNQKTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGSLNDVFI 352
                 K  +S+    V D  G    V   WPGS+ D  VL++S L  +   G   D ++
Sbjct: 171 ------KGLHSLNCLVVCDIRGALMTVETSWPGSLQDCAVLQQSSLSSQFETGMPKDSWL 230

Query: 353 VGNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVK 412
           +G+S + L  WLL P  +   T  ++ +N       +  +     L  R+ CL   ++  
Sbjct: 231 LGDSSFFLHTWLLTPLHIPE-TPAEYRYNRAHSATHSVIEKTLRTLCCRFRCLD-GSKGA 290

Query: 413 LQELPV----VLGACCVLHNI 429
           LQ  P     ++ ACCVLHNI
Sbjct: 291 LQYSPEKSSHIILACCVLHNI 301

BLAST of CSPI01G28370 vs. ExPASy TrEMBL
Match: A0A0A0LYY2 (DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G589710 PE=3 SV=1)

HSP 1 Score: 1001.1 bits (2587), Expect = 1.6e-288
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MEISSFPFLNQEEFLPIFNLFSEMDNPTATFNVNPTSKKRRRSDPNSDDFNSFSFTDEND 60
           MEISSFPFLNQEEFLPIFNLFSEMDNPTATFNVNPTSKKRRRSDPNSDDFNSFSFTDEND
Sbjct: 1   MEISSFPFLNQEEFLPIFNLFSEMDNPTATFNVNPTSKKRRRSDPNSDDFNSFSFTDEND 60

Query: 61  DPTADPLLKLPCWFDPQPESQQNWLMDAQKPKPTNDFHLSDQIPKKPRRASPENPSPVKN 120
           DPTADPLLKLPCWFDPQPESQQNWLMDAQKPKPTNDFHLSDQIPKKPRRASPENPSPVKN
Sbjct: 61  DPTADPLLKLPCWFDPQPESQQNWLMDAQKPKPTNDFHLSDQIPKKPRRASPENPSPVKN 120

Query: 121 TPAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMK 180
           TPAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMK
Sbjct: 121 TPAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMK 180

Query: 181 KDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPK 240
           KDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPK
Sbjct: 181 KDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPK 240

Query: 241 FLNWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTS 300
           FLNWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTS
Sbjct: 241 FLNWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTS 300

Query: 301 YSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGSLNDVFIVGNSGYPLM 360
           YSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGSLNDVFIVGNSGYPLM
Sbjct: 301 YSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGSLNDVFIVGNSGYPLM 360

Query: 361 DWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLG 420
           DWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLG
Sbjct: 361 DWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLG 420

Query: 421 ACCVLHNICEMRKEKFDPELKFEVYDDEMMPENNGLRSVSAIQARDHIAHNLLHHGIAGT 480
           ACCVLHNICEMRKEKFDPELKFEVYDDEMMPENNGLRSVSAIQARDHIAHNLLHHGIAGT
Sbjct: 421 ACCVLHNICEMRKEKFDPELKFEVYDDEMMPENNGLRSVSAIQARDHIAHNLLHHGIAGT 480

Query: 481 GFL 484
           GFL
Sbjct: 481 GFL 483

BLAST of CSPI01G28370 vs. ExPASy TrEMBL
Match: A0A1S3BPN5 (putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103492343 PE=3 SV=1)

HSP 1 Score: 969.9 bits (2506), Expect = 3.9e-279
Identity = 467/484 (96.49%), Postives = 475/484 (98.14%), Query Frame = 0

Query: 1   MEISSFPFLNQEEFLPIFNLFSEMD-NPTATFNVNPTSKKRRRSDPNSDDFNSFSFTDEN 60
           MEISSFPFLNQEEFLPIFNLFS+MD NPT  FNVNPT KKRRRSDPNSDDFN+FSFTDEN
Sbjct: 1   MEISSFPFLNQEEFLPIFNLFSDMDNNPTTPFNVNPTPKKRRRSDPNSDDFNNFSFTDEN 60

Query: 61  DDPTADPLLKLPCWFDPQPESQQNWLMDAQKPKPTNDFHLSDQIPKKPRRASPENPSPVK 120
           D+PT DPLLKLPCWFDPQPES Q+WLMD+QKPKPTNDFHLSDQIPKKPRRASPENPSPVK
Sbjct: 61  DEPTDDPLLKLPCWFDPQPESPQSWLMDSQKPKPTNDFHLSDQIPKKPRRASPENPSPVK 120

Query: 121 NTPAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVM 180
           N PAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVM
Sbjct: 121 NNPAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVM 180

Query: 181 KKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMP 240
           KKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMP
Sbjct: 181 KKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMP 240

Query: 241 KFLNWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKT 300
           KFL WPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAP+NNVAAYFNKRHTERNQKT
Sbjct: 241 KFLQWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPRNNVAAYFNKRHTERNQKT 300

Query: 301 SYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGSLNDVFIVGNSGYPL 360
           SYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMG LNDVF+VGNSGYPL
Sbjct: 301 SYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGLLNDVFVVGNSGYPL 360

Query: 361 MDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVL 420
           MDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVL
Sbjct: 361 MDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVL 420

Query: 421 GACCVLHNICEMRKEKFDPELKFEVYDDEMMPENNGLRSVSAIQARDHIAHNLLHHGIAG 480
           GACCVLHNICEMRKEKFDPELKFEVYDDEM+PENNGLRSVSAIQARDHIAHNLLHHGIAG
Sbjct: 421 GACCVLHNICEMRKEKFDPELKFEVYDDEMLPENNGLRSVSAIQARDHIAHNLLHHGIAG 480

Query: 481 TGFL 484
           TGFL
Sbjct: 481 TGFL 484

BLAST of CSPI01G28370 vs. ExPASy TrEMBL
Match: A0A5D3CDK0 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001910 PE=3 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 1.7e-266
Identity = 444/459 (96.73%), Postives = 451/459 (98.26%), Query Frame = 0

Query: 25  DNPTATFNVNPTSKKRRRSDPNSDDFNSFSFTDENDDPTADPLLKLPCWFDPQPESQQNW 84
           +NPT  FNVNPT KKRRRSDPNSDDFN+FSFTDEND+PT DPLLKLPCWFDPQPES Q+W
Sbjct: 3   NNPTTPFNVNPTPKKRRRSDPNSDDFNNFSFTDENDEPTDDPLLKLPCWFDPQPESPQSW 62

Query: 85  LMDAQKPKPTNDFHLSDQIPKKPRRASPENPSPVKNTPAGGGGTQQRRLWVKDRSKDWWD 144
           LMD+QKPKPTNDFHLSDQIPKKPRRASPENPSPVKN PAGGGGTQQRRLWVKDRSKDWWD
Sbjct: 63  LMDSQKPKPTNDFHLSDQIPKKPRRASPENPSPVKNNPAGGGGTQQRRLWVKDRSKDWWD 122

Query: 145 QCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLAT 204
           QCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLAT
Sbjct: 123 QCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLAT 182

Query: 205 GEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLNWPDESKLTKIKQEFESISGIP 264
           GEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFL WPDESKLTKIKQEFESISGIP
Sbjct: 183 GEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIKQEFESISGIP 242

Query: 265 KVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPSGVFTDVCIGWPG 324
           KVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPSGVFTDVCIGWPG
Sbjct: 243 KVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPSGVFTDVCIGWPG 302

Query: 325 SMPDDQVLEKSLLYERASMGSLNDVFIVGNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVG 384
           SMPDDQVLEKSLLYERASMG LNDVF+VGNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVG
Sbjct: 303 SMPDDQVLEKSLLYERASMGLLNDVFVVGNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVG 362

Query: 385 EIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICEMRKEKFDPELKFEV 444
           EIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICEMRKEKFDPELKFEV
Sbjct: 363 EIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICEMRKEKFDPELKFEV 422

Query: 445 YDDEMMPENNGLRSVSAIQARDHIAHNLLHHGIAGTGFL 484
           YDDEM+PENNGLRSVSAIQARDHIAHNLLHHGIAGTGFL
Sbjct: 423 YDDEMLPENNGLRSVSAIQARDHIAHNLLHHGIAGTGFL 461

BLAST of CSPI01G28370 vs. ExPASy TrEMBL
Match: A0A6J1F642 (protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111441167 PE=3 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 2.0e-227
Identity = 398/489 (81.39%), Postives = 427/489 (87.32%), Query Frame = 0

Query: 1   MEISSFPFLNQEEFLPIFNLFSEMDNPTATFNVNPTSKKRRRSDPNSDD----FNSFSFT 60
           MEISSFPFLNQ++ LPIFNLFSEMD+    F+VN + KKRRR + + DD    FN  SF 
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDD---NFSVNQSPKKRRRQNDDDDDHQTQFNKTSFD 60

Query: 61  DENDDPTADPLLKLPCWFDPQPESQQNWLMDAQKPKPTNDFHLSDQIPKKPRRASPEN-- 120
           D       D LLKLP WFD   + QQ+W+M+ +     +D +L+  +PKKPRRA+PEN  
Sbjct: 61  D-------DELLKLPFWFD--DDKQQHWIMEQKPEFQVSDENLTQFVPKKPRRATPENTH 120

Query: 121 PSPVKNTPAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKEL 180
            SP K    GG GTQ RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+EL
Sbjct: 121 SSPAK----GGAGTQHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQEL 180

Query: 181 DSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIR 240
           DSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIR
Sbjct: 181 DSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIR 240

Query: 241 KVLMPKFLNWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTE 300
           KVLMPKFL WP+ESKL KIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTE
Sbjct: 241 KVLMPKFLQWPEESKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTE 300

Query: 301 RNQKTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGSLNDVFIVGN 360
           RNQKTSYSITVQGVVDP+GVFTDVCIGWPGSMPDDQVLEKS L+ERA+MG L DV IVGN
Sbjct: 301 RNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGN 360

Query: 361 SGYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQE 420
           SGYPL DWLLVPY+ QNLTWTQH FNEKV EIQ AAKAAFGRLKGRWTCLQKRTEVKLQE
Sbjct: 361 SGYPLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQE 420

Query: 421 LPVVLGACCVLHNICEMRKEKFDPELKFEVYDDEMMPENNGLRSVSAIQARDHIAHNLLH 480
           LPVVLGACCVLHNICEMRKE+FDPELKFE +DDEM+PENNG+RS SAIQARDHIAHNLLH
Sbjct: 421 LPVVLGACCVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLH 473

Query: 481 HGIAGTGFL 484
           HG+AGTGFL
Sbjct: 481 HGLAGTGFL 473

BLAST of CSPI01G28370 vs. ExPASy TrEMBL
Match: A0A6J1HRQ5 (protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111466889 PE=3 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 4.2e-225
Identity = 393/485 (81.03%), Postives = 424/485 (87.42%), Query Frame = 0

Query: 1   MEISSFPFLNQEEFLPIFNLFSEMDNPTATFNVNPTSKKRRRSDPNSDDFNSFSFTDEND 60
           MEISSFPFLNQ++ LPIFNLFSEMD+    F+VN + KKRRR D + DD   F+ T  +D
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDD---NFSVNYSPKKRRRQDDDDDDQTQFNKTSFDD 60

Query: 61  DPTADPLLKLPCWFDPQPESQQNWLMDAQKPKPTNDFHLSDQIPKKPRRASPEN--PSPV 120
               D LLKLP WFD   + QQ+W+M+ +      D +L+  + KKPRRA+PEN   SP 
Sbjct: 61  ----DELLKLPFWFD--DDKQQHWIMEQKPEFQVFDENLTQFVAKKPRRATPENTHSSPA 120

Query: 121 KNTPAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTV 180
           K    GG GTQ RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+ELDSTV
Sbjct: 121 K----GGAGTQHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTV 180

Query: 181 MKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLM 240
           MKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLM
Sbjct: 181 MKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLM 240

Query: 241 PKFLNWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQK 300
           PKFL WP++SKL KIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQK
Sbjct: 241 PKFLQWPEDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQK 300

Query: 301 TSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGSLNDVFIVGNSGYP 360
           TSYSITVQGVVDP+GVFTDVCIGWPGSMPDDQVLEKS L+ERA+MG L DV IVGNSGYP
Sbjct: 301 TSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYP 360

Query: 361 LMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVV 420
           L DWLLVPY+ QNLTWTQH FNEKV EIQ AAKAAFGRLKGRWTCLQKRTEVKLQELPVV
Sbjct: 361 LTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVV 420

Query: 421 LGACCVLHNICEMRKEKFDPELKFEVYDDEMMPENNGLRSVSAIQARDHIAHNLLHHGIA 480
           LGACCVLHNICEMRKE+FDPELKFE +DDEM+PENNG+RS SAI ARDHI+HNLLHHG+A
Sbjct: 421 LGACCVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAILARDHISHNLLHHGLA 472

Query: 481 GTGFL 484
           GTGFL
Sbjct: 481 GTGFL 472

BLAST of CSPI01G28370 vs. NCBI nr
Match: XP_004144012.1 (protein ALP1-like [Cucumis sativus])

HSP 1 Score: 1001.1 bits (2587), Expect = 3.2e-288
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MEISSFPFLNQEEFLPIFNLFSEMDNPTATFNVNPTSKKRRRSDPNSDDFNSFSFTDEND 60
           MEISSFPFLNQEEFLPIFNLFSEMDNPTATFNVNPTSKKRRRSDPNSDDFNSFSFTDEND
Sbjct: 1   MEISSFPFLNQEEFLPIFNLFSEMDNPTATFNVNPTSKKRRRSDPNSDDFNSFSFTDEND 60

Query: 61  DPTADPLLKLPCWFDPQPESQQNWLMDAQKPKPTNDFHLSDQIPKKPRRASPENPSPVKN 120
           DPTADPLLKLPCWFDPQPESQQNWLMDAQKPKPTNDFHLSDQIPKKPRRASPENPSPVKN
Sbjct: 61  DPTADPLLKLPCWFDPQPESQQNWLMDAQKPKPTNDFHLSDQIPKKPRRASPENPSPVKN 120

Query: 121 TPAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMK 180
           TPAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMK
Sbjct: 121 TPAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMK 180

Query: 181 KDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPK 240
           KDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPK
Sbjct: 181 KDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPK 240

Query: 241 FLNWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTS 300
           FLNWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTS
Sbjct: 241 FLNWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTS 300

Query: 301 YSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGSLNDVFIVGNSGYPLM 360
           YSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGSLNDVFIVGNSGYPLM
Sbjct: 301 YSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGSLNDVFIVGNSGYPLM 360

Query: 361 DWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLG 420
           DWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLG
Sbjct: 361 DWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLG 420

Query: 421 ACCVLHNICEMRKEKFDPELKFEVYDDEMMPENNGLRSVSAIQARDHIAHNLLHHGIAGT 480
           ACCVLHNICEMRKEKFDPELKFEVYDDEMMPENNGLRSVSAIQARDHIAHNLLHHGIAGT
Sbjct: 421 ACCVLHNICEMRKEKFDPELKFEVYDDEMMPENNGLRSVSAIQARDHIAHNLLHHGIAGT 480

Query: 481 GFL 484
           GFL
Sbjct: 481 GFL 483

BLAST of CSPI01G28370 vs. NCBI nr
Match: KAE8653438.1 (hypothetical protein Csa_007306 [Cucumis sativus])

HSP 1 Score: 1001.1 bits (2587), Expect = 3.2e-288
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MEISSFPFLNQEEFLPIFNLFSEMDNPTATFNVNPTSKKRRRSDPNSDDFNSFSFTDEND 60
           MEISSFPFLNQEEFLPIFNLFSEMDNPTATFNVNPTSKKRRRSDPNSDDFNSFSFTDEND
Sbjct: 72  MEISSFPFLNQEEFLPIFNLFSEMDNPTATFNVNPTSKKRRRSDPNSDDFNSFSFTDEND 131

Query: 61  DPTADPLLKLPCWFDPQPESQQNWLMDAQKPKPTNDFHLSDQIPKKPRRASPENPSPVKN 120
           DPTADPLLKLPCWFDPQPESQQNWLMDAQKPKPTNDFHLSDQIPKKPRRASPENPSPVKN
Sbjct: 132 DPTADPLLKLPCWFDPQPESQQNWLMDAQKPKPTNDFHLSDQIPKKPRRASPENPSPVKN 191

Query: 121 TPAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMK 180
           TPAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMK
Sbjct: 192 TPAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMK 251

Query: 181 KDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPK 240
           KDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPK
Sbjct: 252 KDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPK 311

Query: 241 FLNWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTS 300
           FLNWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTS
Sbjct: 312 FLNWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTS 371

Query: 301 YSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGSLNDVFIVGNSGYPLM 360
           YSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGSLNDVFIVGNSGYPLM
Sbjct: 372 YSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGSLNDVFIVGNSGYPLM 431

Query: 361 DWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLG 420
           DWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLG
Sbjct: 432 DWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLG 491

Query: 421 ACCVLHNICEMRKEKFDPELKFEVYDDEMMPENNGLRSVSAIQARDHIAHNLLHHGIAGT 480
           ACCVLHNICEMRKEKFDPELKFEVYDDEMMPENNGLRSVSAIQARDHIAHNLLHHGIAGT
Sbjct: 492 ACCVLHNICEMRKEKFDPELKFEVYDDEMMPENNGLRSVSAIQARDHIAHNLLHHGIAGT 551

Query: 481 GFL 484
           GFL
Sbjct: 552 GFL 554

BLAST of CSPI01G28370 vs. NCBI nr
Match: XP_008450882.1 (PREDICTED: putative nuclease HARBI1 [Cucumis melo])

HSP 1 Score: 969.9 bits (2506), Expect = 8.0e-279
Identity = 467/484 (96.49%), Postives = 475/484 (98.14%), Query Frame = 0

Query: 1   MEISSFPFLNQEEFLPIFNLFSEMD-NPTATFNVNPTSKKRRRSDPNSDDFNSFSFTDEN 60
           MEISSFPFLNQEEFLPIFNLFS+MD NPT  FNVNPT KKRRRSDPNSDDFN+FSFTDEN
Sbjct: 1   MEISSFPFLNQEEFLPIFNLFSDMDNNPTTPFNVNPTPKKRRRSDPNSDDFNNFSFTDEN 60

Query: 61  DDPTADPLLKLPCWFDPQPESQQNWLMDAQKPKPTNDFHLSDQIPKKPRRASPENPSPVK 120
           D+PT DPLLKLPCWFDPQPES Q+WLMD+QKPKPTNDFHLSDQIPKKPRRASPENPSPVK
Sbjct: 61  DEPTDDPLLKLPCWFDPQPESPQSWLMDSQKPKPTNDFHLSDQIPKKPRRASPENPSPVK 120

Query: 121 NTPAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVM 180
           N PAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVM
Sbjct: 121 NNPAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVM 180

Query: 181 KKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMP 240
           KKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMP
Sbjct: 181 KKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMP 240

Query: 241 KFLNWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKT 300
           KFL WPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAP+NNVAAYFNKRHTERNQKT
Sbjct: 241 KFLQWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPRNNVAAYFNKRHTERNQKT 300

Query: 301 SYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGSLNDVFIVGNSGYPL 360
           SYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMG LNDVF+VGNSGYPL
Sbjct: 301 SYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGLLNDVFVVGNSGYPL 360

Query: 361 MDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVL 420
           MDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVL
Sbjct: 361 MDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVL 420

Query: 421 GACCVLHNICEMRKEKFDPELKFEVYDDEMMPENNGLRSVSAIQARDHIAHNLLHHGIAG 480
           GACCVLHNICEMRKEKFDPELKFEVYDDEM+PENNGLRSVSAIQARDHIAHNLLHHGIAG
Sbjct: 421 GACCVLHNICEMRKEKFDPELKFEVYDDEMLPENNGLRSVSAIQARDHIAHNLLHHGIAG 480

Query: 481 TGFL 484
           TGFL
Sbjct: 481 TGFL 484

BLAST of CSPI01G28370 vs. NCBI nr
Match: KAA0055806.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa] >TYK10057.1 putative nuclease HARBI1 [Cucumis melo var. makuwa])

HSP 1 Score: 927.9 bits (2397), Expect = 3.5e-266
Identity = 444/459 (96.73%), Postives = 451/459 (98.26%), Query Frame = 0

Query: 25  DNPTATFNVNPTSKKRRRSDPNSDDFNSFSFTDENDDPTADPLLKLPCWFDPQPESQQNW 84
           +NPT  FNVNPT KKRRRSDPNSDDFN+FSFTDEND+PT DPLLKLPCWFDPQPES Q+W
Sbjct: 3   NNPTTPFNVNPTPKKRRRSDPNSDDFNNFSFTDENDEPTDDPLLKLPCWFDPQPESPQSW 62

Query: 85  LMDAQKPKPTNDFHLSDQIPKKPRRASPENPSPVKNTPAGGGGTQQRRLWVKDRSKDWWD 144
           LMD+QKPKPTNDFHLSDQIPKKPRRASPENPSPVKN PAGGGGTQQRRLWVKDRSKDWWD
Sbjct: 63  LMDSQKPKPTNDFHLSDQIPKKPRRASPENPSPVKNNPAGGGGTQQRRLWVKDRSKDWWD 122

Query: 145 QCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLAT 204
           QCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLAT
Sbjct: 123 QCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLAT 182

Query: 205 GEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLNWPDESKLTKIKQEFESISGIP 264
           GEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFL WPDESKLTKIKQEFESISGIP
Sbjct: 183 GEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIKQEFESISGIP 242

Query: 265 KVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPSGVFTDVCIGWPG 324
           KVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPSGVFTDVCIGWPG
Sbjct: 243 KVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPSGVFTDVCIGWPG 302

Query: 325 SMPDDQVLEKSLLYERASMGSLNDVFIVGNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVG 384
           SMPDDQVLEKSLLYERASMG LNDVF+VGNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVG
Sbjct: 303 SMPDDQVLEKSLLYERASMGLLNDVFVVGNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVG 362

Query: 385 EIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICEMRKEKFDPELKFEV 444
           EIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICEMRKEKFDPELKFEV
Sbjct: 363 EIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICEMRKEKFDPELKFEV 422

Query: 445 YDDEMMPENNGLRSVSAIQARDHIAHNLLHHGIAGTGFL 484
           YDDEM+PENNGLRSVSAIQARDHIAHNLLHHGIAGTGFL
Sbjct: 423 YDDEMLPENNGLRSVSAIQARDHIAHNLLHHGIAGTGFL 461

BLAST of CSPI01G28370 vs. NCBI nr
Match: XP_038880089.1 (protein ALP1-like [Benincasa hispida])

HSP 1 Score: 859.4 bits (2219), Expect = 1.5e-245
Identity = 428/494 (86.64%), Postives = 450/494 (91.09%), Query Frame = 0

Query: 1   MEISSFPFLNQEEFLPIFNLFSEMD----NPTATFNVNPTSKKRRRSDPNSDD---FNSF 60
           MEISSFPFLNQEE LPIFNLFS+MD    N  ATF+VN + KKRRRSD N DD   FN+ 
Sbjct: 1   MEISSFPFLNQEELLPIFNLFSDMDNNHNNTNATFSVNQSPKKRRRSDENGDDHSQFNNI 60

Query: 61  SFTDENDDPTADPLLKLPCWFDPQPESQQNWLMDAQKPKPTNDFHLSDQIP----KKPRR 120
           SFT END    + L KLPCWF    ESQ+NW+MD+++PKP N+FHLSDQ P    KKPRR
Sbjct: 61  SFT-END----EALQKLPCWF----ESQENWIMDSEEPKPRNEFHLSDQNPTQFSKKPRR 120

Query: 121 ASPENPSPVKNTPAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDM 180
            + EN SP KN    GGG QQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDM
Sbjct: 121 TTAENGSPAKNPT--GGGAQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDM 180

Query: 181 ICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEV 240
           ICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEV
Sbjct: 181 ICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEV 240

Query: 241 CSAIRKVLMPKFLNWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFN 300
           CSAIRKVLMPKFL WPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFN
Sbjct: 241 CSAIRKVLMPKFLQWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFN 300

Query: 301 KRHTERNQKTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGSLNDV 360
           KRHTERNQKTSYSITVQGVVDP+GVFTDVCIGWPGSMPDDQVLEKS+L+ERA+MG LNDV
Sbjct: 301 KRHTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSVLFERANMGLLNDV 360

Query: 361 FIVGNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTE 420
           FIVGNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAK AFGRLKGRW+CLQKRTE
Sbjct: 361 FIVGNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTE 420

Query: 421 VKLQELPVVLGACCVLHNICEMRKEKFDPELKFEVYDDEMMPENNGLRSVSAIQARDHIA 480
           VKLQELPVVLGACCVLHNICE+RKEKFDP+LKFE+YDDEM+PENNGLRSVSAIQARDHIA
Sbjct: 421 VKLQELPVVLGACCVLHNICEIRKEKFDPDLKFELYDDEMVPENNGLRSVSAIQARDHIA 480

Query: 481 HNLLHHGIAGTGFL 484
           HNLLHHG+AGTGFL
Sbjct: 481 HNLLHHGLAGTGFL 483

BLAST of CSPI01G28370 vs. TAIR 10
Match: AT5G12010.1 (unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, plasma membrane, membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G29780.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 571.2 bits (1471), Expect = 7.7e-163
Identity = 266/361 (73.68%), Postives = 313/361 (86.70%), Query Frame = 0

Query: 124 GGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDT 183
           G G  QQRRLWVKDRS+ WW++C+  D+P+E+F++AFRMSKSTF++IC EL+S V K+DT
Sbjct: 143 GTGSGQQRRLWVKDRSRAWWEECSRLDYPEEDFKKAFRMSKSTFELICDELNSAVAKEDT 202

Query: 184 MLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLN 243
            LR AIPVRQRVAVCIWRLATGEPLRLVSK+FGLGISTCHKLVLEVC AI+ VLMPK+L 
Sbjct: 203 ALRNAIPVRQRVAVCIWRLATGEPLRLVSKKFGLGISTCHKLVLEVCKAIKDVLMPKYLQ 262

Query: 244 WPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSI 303
           WPD+  L  I++ FES+SGIP V GS+YTTHIPIIAPK +VA+YFNKRHTERNQKTSYSI
Sbjct: 263 WPDDESLRNIRERFESVSGIPNVVGSMYTTHIPIIAPKISVASYFNKRHTERNQKTSYSI 322

Query: 304 TVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGS-LNDVFIVGNSGYPLMDW 363
           T+Q VV+P GVFTD+CIGWPGSMPDD+VLEKSLLY+RA+ G  L  +++ G  G+PL+DW
Sbjct: 323 TIQAVVNPKGVFTDLCIGWPGSMPDDKVLEKSLLYQRANNGGLLKGMWVAGGPGHPLLDW 382

Query: 364 LLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGAC 423
           +LVPYT QNLTWTQH FNEK+ E+Q  AK AFGRLKGRW CLQKRTEVKLQ+LP VLGAC
Sbjct: 383 VLVPYTQQNLTWTQHAFNEKMSEVQGVAKEAFGRLKGRWACLQKRTEVKLQDLPTVLGAC 442

Query: 424 CVLHNICEMRKEKFDPELKFEVYDDEMMPENNGLRSVSAIQARDHIAHNLLHHGIAGTGF 483
           CVLHNICEMR+EK +PEL  EV DDE++PE N LRSV+A++ARD I+HNLLHHG+AGT F
Sbjct: 443 CVLHNICEMREEKMEPELMVEVIDDEVLPE-NVLRSVNAMKARDTISHNLLHHGLAGTSF 502

BLAST of CSPI01G28370 vs. TAIR 10
Match: AT4G29780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12010.1); Has 945 Blast hits to 944 proteins in 87 species: Archae - 0; Bacteria - 0; Metazoa - 519; Fungi - 43; Plants - 365; Viruses - 0; Other Eukaryotes - 18 (source: NCBI BLink). )

HSP 1 Score: 537.7 bits (1384), Expect = 9.4e-153
Identity = 298/541 (55.08%), Postives = 356/541 (65.80%), Query Frame = 0

Query: 1   MEISSFPF--LNQEEFLPIFNLFSEMDNPTATF-------NVNPTSKKRRRSDPNSDDFN 60
           MEISSFPF  L  +E      LF +MD+  +TF       N N T++K+R   P  DD  
Sbjct: 1   MEISSFPFPYLQDDECSHFLGLFQDMDSSPSTFGLEGFNSNDNNTNQKKR---PRKDDEG 60

Query: 61  S----------FSFTDENDDPTADPLLKLPCWFDPQPESQQNW---------LMDAQKPK 120
                       +    N     D L  L    +   + Q+ W         L++A   K
Sbjct: 61  GGGGGGGTEVLGAVNGNNKAAFGDILATLLLLDEEAKQQQEQWDFEFIKEKSLLEANHKK 120

Query: 121 PTNDF---------HLS------DQIPKKPRRASP----------------ENPSPVKNT 180
                         H S          K+ R+ +                   P P  + 
Sbjct: 121 KVKTMDGYYNQMQDHYSAAGETDGSRSKRARKTAVAAVVSAVASGADTTGLAAPVPTADI 180

Query: 181 PAG-GGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMK 240
            +G G G   RRLWVK+R+ DWWD+ + PDFP++EFRR FRMSKSTF++IC+ELD+TV K
Sbjct: 181 ASGSGSGPSHRRLWVKERTTDWWDRVSRPDFPEDEFRREFRMSKSTFNLICEELDTTVTK 240

Query: 241 KDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPK 300
           K+TMLR AIP  +RV VC+WRLATG PLR VS+RFGLGISTCHKLV+EVC AI  VLMPK
Sbjct: 241 KNTMLRDAIPAPKRVGVCVWRLATGAPLRHVSERFGLGISTCHKLVIEVCRAIYDVLMPK 300

Query: 301 FLNWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTS 360
           +L WP +S++   K +FES+  IP V GSIYTTHIPIIAPK +VAAYFNKRHTERNQKTS
Sbjct: 301 YLLWPSDSEINSTKAKFESVHKIPNVVGSIYTTHIPIIAPKVHVAAYFNKRHTERNQKTS 360

Query: 361 YSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEK-SLLYERASMGSLNDVFIVGNSGYPL 420
           YSITVQGVV+  G+FTDVCIG PGS+ DDQ+LEK SL  +RA+ G L D +IVGNSG+PL
Sbjct: 361 YSITVQGVVNADGIFTDVCIGNPGSLTDDQILEKSSLSRQRAARGMLRDSWIVGNSGFPL 420

Query: 421 MDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVL 480
            D+LLVPYT QNLTWTQH FNE +GEIQ  A AAF RLKGRW CLQKRTEVKLQ+LP VL
Sbjct: 421 TDYLLVPYTRQNLTWTQHAFNESIGEIQGIATAAFERLKGRWACLQKRTEVKLQDLPYVL 480

BLAST of CSPI01G28370 vs. TAIR 10
Match: AT3G55350.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 162.9 bits (411), Expect = 6.3e-40
Identity = 112/362 (30.94%), Postives = 176/362 (48.62%), Query Frame = 0

Query: 138 RSKDWWDQCNHPDF----PDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVA----I 197
           +S DWWD  +   +      + F   F++S+ TFD IC  + +    K      +    +
Sbjct: 50  QSLDWWDGFSRRIYGGSTDPKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPL 109

Query: 198 PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLNWPDESK 257
            +  RVAV + RL +GE L ++ + FG+  ST  ++      ++ +  +   L+WP  SK
Sbjct: 110 SLNDRVAVALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAI-HHLSWP--SK 169

Query: 258 LTKIKQEFESISGIPKVGGSIYTTHI----PIIAPKNNVAAYFNKRHTERNQKTSYSITV 317
           L +IK +FE ISG+P   G+I  THI    P + P N V           + + ++S+T+
Sbjct: 170 LDEIKSKFEKISGLPNCCGAIDITHIVMNLPAVEPSNKVWL---------DGEKNFSMTL 229

Query: 318 QGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGS-LND------------VFIV 377
           Q VVDP   F DV  GWPGS+ DD VL+ S  Y+    G  LN              +IV
Sbjct: 230 QAVVDPDMRFLDVIAGWPGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIV 289

Query: 378 GNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEV-K 437
           G+SG+PL+ WLL PY  +  +  Q  FN++  E   AA+ A  +LK RW  +     +  
Sbjct: 290 GDSGFPLLPWLLTPYQGKPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPD 349

Query: 438 LQELPVVLGACCVLHN-ICEMRKEKFDPELKFEVYDDEMMPENNGLRSVSAIQARDHIAH 473
              LP ++  CC+LHN I +M  +  D +   + +D      +  L   ++   RD ++ 
Sbjct: 350 RNRLPRIIFVCCLLHNIIIDMEDQTLDDQPLSQQHDMNYRQRSCKLADEASSVLRDELSD 399

BLAST of CSPI01G28370 vs. TAIR 10
Match: AT3G63270.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: PIF / Ping-Pong family of plant transposases (TAIR:AT3G55350.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 145.6 bits (366), Expect = 1.0e-34
Identity = 93/314 (29.62%), Postives = 156/314 (49.68%), Query Frame = 0

Query: 141 DWWD----QCNHPDFPDEE---FRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAI---- 200
           DWWD    + + P  P +E   F+  FR SK+TF  IC  +   ++ +     + I    
Sbjct: 43  DWWDTFWLRNSSPSVPSDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRL 102

Query: 201 -PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLNWPDES 260
             V ++VA+ + RLA+G+    V   FG+G ST  ++      A+ +      L WPD  
Sbjct: 103 LSVEKQVAIALRRLASGDSQVSVGAAFGVGQSTVSQVTWRFIEALEE-RAKHHLRWPDSD 162

Query: 261 KLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGV 320
           ++ +IK +FE + G+P   G+I TTHI +  P    +  +       +Q+ +YS+ +QGV
Sbjct: 163 RIEEIKSKFEEMYGLPNCCGAIDTTHIIMTLPAVQASDDW------CDQEKNYSMFLQGV 222

Query: 321 VDPSGVFTDVCIGWPGSMPDDQVLEKSLLY-------------ERASMGSLNDVFIVGNS 380
            D    F ++  GWPG M   ++L+ S  +             +  S G+    ++VG  
Sbjct: 223 FDHEMRFLNMVTGWPGGMTVSKLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGI 282

Query: 381 GYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQK-RTEVKLQE 429
            YPL+ WL+ P+   + + +   FNE+  ++++ A  AF +LKG W  L K       ++
Sbjct: 283 SYPLLPWLITPHDSDHPSDSMVAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRK 342

BLAST of CSPI01G28370 vs. TAIR 10
Match: AT3G19120.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 107.8 bits (268), Expect = 2.4e-23
Identity = 89/352 (25.28%), Postives = 155/352 (44.03%), Query Frame = 0

Query: 110 ASPENPSPVKNTPAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDM 169
           +S E+PSP    P   G               W    + P   D  +R  + +S   F  
Sbjct: 74  SSSESPSPSPPPPLADGDYSVAAFRALTTDHIW--SLDAP-LRDARWRSLYGLSYPVFIT 133

Query: 170 ICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEV 229
           +  +L   +    T   +++P    VA+ + RLA G   + ++ R+ L      K+   V
Sbjct: 134 VVDKLKPFI----TASNLSLPADYAVAMVLSRLAHGCSAKTLASRYSLDPYLISKITNMV 193

Query: 230 CSAIRKVLMPKFLNWP-DESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYF 289
              +   L P+F+  P  + +L +  Q FE ++ +P + G+I +T + +           
Sbjct: 194 TRLLATKLYPEFIKIPVGKRRLIETTQGFEELTSLPNICGAIDSTPVKL----------- 253

Query: 290 NKRHTERNQKTSY-------SITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERA 349
            +R T+ N +  Y       ++ +Q V D   +F DVC+  PG   D      SLLY+R 
Sbjct: 254 -RRRTKLNPRNIYGCKYGYDAVLLQVVADHKKIFWDVCVKAPGGEDDSSHFRDSLLYKRL 313

Query: 350 SMGSL------------NDVFIVGNSGYPLMDWLLVPYTVQNL-TWTQHGFNEKVGEIQA 409
           + G +               +IVG+  YPL+ +L+ P++     T  ++ F+  + + ++
Sbjct: 314 TSGDIVWEKVINIRGHHVRPYIVGDWCYPLLSFLMTPFSPNGSGTPPENLFDGMLMKGRS 373

Query: 410 AAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICEMRKEKFDPEL 441
               A G LK RW  LQ    V +   P  + ACCVLHN+C++ +E  +PE+
Sbjct: 374 VVVEAIGLLKARWKILQS-LNVGVNHAPQTIVACCVLHNLCQIAREP-EPEI 404

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M2U38.9e-3930.94Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1[more]
Q94K491.5e-3329.62Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=... [more]
Q96MB77.3e-1726.44Putative nuclease HARBI1 OS=Homo sapiens OX=9606 GN=HARBI1 PE=1 SV=1[more]
Q17QR81.6e-1626.05Putative nuclease HARBI1 OS=Bos taurus OX=9913 GN=HARBI1 PE=2 SV=1[more]
B0BN951.6e-1626.05Putative nuclease HARBI1 OS=Rattus norvegicus OX=10116 GN=Harbi1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LYY21.6e-288100.00DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G589710 PE... [more]
A0A1S3BPN53.9e-27996.49putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103492343 PE=3 SV=1[more]
A0A5D3CDK01.7e-26696.73Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A6J1F6422.0e-22781.39protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111441167 PE=3 SV=1[more]
A0A6J1HRQ54.2e-22581.03protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111466889 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004144012.13.2e-288100.00protein ALP1-like [Cucumis sativus][more]
KAE8653438.13.2e-288100.00hypothetical protein Csa_007306 [Cucumis sativus][more]
XP_008450882.18.0e-27996.49PREDICTED: putative nuclease HARBI1 [Cucumis melo][more]
KAA0055806.13.5e-26696.73putative nuclease HARBI1 [Cucumis melo var. makuwa] >TYK10057.1 putative nucleas... [more]
XP_038880089.11.5e-24586.64protein ALP1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G12010.17.7e-16373.68unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, ... [more]
AT4G29780.19.4e-15355.08unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G55350.16.3e-4030.94PIF / Ping-Pong family of plant transposases [more]
AT3G63270.11.0e-3429.62CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int... [more]
AT3G19120.12.4e-2325.28PIF / Ping-Pong family of plant transposases [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027806Harbinger transposase-derived nuclease domainPFAMPF13359DDE_Tnp_4coord: 270..427
e-value: 2.8E-35
score: 121.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 88..131
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 26..72
NoneNo IPR availablePANTHERPTHR22930:SF199NUCLEASEcoord: 119..482
NoneNo IPR availablePANTHERPTHR22930UNCHARACTERIZEDcoord: 119..482

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G28370.1CSPI01G28370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding