CSPI01G27250 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G27250
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPentatricopeptide repeat-containing protein
LocationChr1: 22150807 .. 22152394 (-)
RNA-Seq ExpressionCSPI01G27250
SyntenyCSPI01G27250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTTACTTAAGCAATCAAAGATTGGATACTATGACGGTATAAATGTGGCACAAAGATTGGATACGAATGCAACCTCGCAAATGCACCTGGCCCCAAGACTGAGCTGCATCCACCACCTAAGTAACGTAAGAATTTCTTCCCTTCAACTTCTACAAATTCATGCCCAATTGATAACCAATGGCTTCAAATCTCCCTCCCCTTACGCCAAGCTAATCACCCATTTATGCAAGAAATCTTCCTCAGAATCCATCGCCCACGCCCATTTGATCTTCCGGCACCACCGATACTCTCCAAATCTCTTCCTCTTCAACACTCTCATAAGATGCGCTCCACCTCATCATTCCATCTCTATTTTCGCCACTTGGGTCTCCACCTCCCACTTCGAATTTGACGATTTCACTTTCATTTTCGTGCTTGGAGCTTGCGCGCGAGCCCCATCTGTTTCTACGTTAATGATTGGTAGGCAAATTCATACTCATATTCTTAAACGTGGGATTGTTTCGAACATTTGGGTGCAGACTACTATGATACATTTTTATTCCATTAATAAAGGTGTGGGTAGTGCGCGGAAGTTGTTTGATGAAATGTCTTTGAGAAATAGTGTTACTTGGAATGCGATGATTGCAGGGTACTGCTCACAAGGTGGTAAGGTTTCTCAGAAATATGCCCGAGATGCGTTGGAATTGTTTCGGGGGATGTTGGTTGAATCAACGAATTTTGAGGTGAAACCAACGGATACTACAATGGTTTGCATTCTTTCAGCTGCATCTCAACTAGGTATGCTTGAAACCGGTTCTTGTGTTCATGCTTATATCAAGAAGACAGTTGATTCCCCTGAAAAAGATGTGTTTATTGGCACTGGCTTGGTTAATATGTACTCAAAATGTGGGCTTCTTAACAGTGCCTCGTCAGTTTTTAAGCAGATGAAGCAGAGGAACGTTTTGACATGGACATCCATGGCGACAGGACTGGCTGTTCATGGAAGGGGTAAAGAAGCATTGGAGCTATTGGATGCAATGGGAGCTCATGGTGTAAAGCCAAACGCAGTAACTTTCACGAGTTTGCTTTCTGCTTGCTGTCATGGAGGGCTTATTGAAGAGGGGCTCCATTTGTTTCGTGTCATGGAGAGGAAGTTTGGTGTTGTGCCTCAAATGCAGCATTATGGCTGCATTGTTGACCTTCTTGGGCGCTCTGGGCACTTGAGAGAGGCATACAAATTGATACTCGAAATGCCAATGGAACCTGATGGTGTTTTATGGAGGAGTTTGCTGAGTTCTTGTATGCTCCATGGTGATGTTGAAATGGGAGAGAGAGTGGGTAAGTTGCTTGTTGAGAGACAGGGAGGGGAGAGTTTTGATGACGAATGGTGTGTTGGAAGTGAGGACTTTGTAGCTTTGTCAAATGTTTATGCTTCTGCCGAAAGGTGGGACGATGTGGAGGCTTTGAGAGATGAAATGAAGATCAAAGGGATTGAAAACAAAGCTGGGTGTAGTTCACTACAAACTACGGGTTCTCAAGGCTTGGTGGAGGCTTTATTATAGGTATTGTACAATTAGTTTCAATTTATAAAACTGTTAATGTGGACGGT

mRNA sequence

GCTTACTTAAGCAATCAAAGATTGGATACTATGACGGTATAAATGTGGCACAAAGATTGGATACGAATGCAACCTCGCAAATGCACCTGGCCCCAAGACTGAGCTGCATCCACCACCTAAGTAACGTAAGAATTTCTTCCCTTCAACTTCTACAAATTCATGCCCAATTGATAACCAATGGCTTCAAATCTCCCTCCCCTTACGCCAAGCTAATCACCCATTTATGCAAGAAATCTTCCTCAGAATCCATCGCCCACGCCCATTTGATCTTCCGGCACCACCGATACTCTCCAAATCTCTTCCTCTTCAACACTCTCATAAGATGCGCTCCACCTCATCATTCCATCTCTATTTTCGCCACTTGGGTCTCCACCTCCCACTTCGAATTTGACGATTTCACTTTCATTTTCGTGCTTGGAGCTTGCGCGCGAGCCCCATCTGTTTCTACGTTAATGATTGGTAGGCAAATTCATACTCATATTCTTAAACGTGGGATTGTTTCGAACATTTGGGTGCAGACTACTATGATACATTTTTATTCCATTAATAAAGGTGTGGGTAGTGCGCGGAAGTTGTTTGATGAAATGTCTTTGAGAAATAGTGTTACTTGGAATGCGATGATTGCAGGGTACTGCTCACAAGGTGGTAAGGTTTCTCAGAAATATGCCCGAGATGCGTTGGAATTGTTTCGGGGGATGTTGGTTGAATCAACGAATTTTGAGGTGAAACCAACGGATACTACAATGGTTTGCATTCTTTCAGCTGCATCTCAACTAGGTATGCTTGAAACCGGTTCTTGTGTTCATGCTTATATCAAGAAGACAGTTGATTCCCCTGAAAAAGATGTGTTTATTGGCACTGGCTTGGTTAATATGTACTCAAAATGTGGGCTTCTTAACAGTGCCTCGTCAGTTTTTAAGCAGATGAAGCAGAGGAACGTTTTGACATGGACATCCATGGCGACAGGACTGGCTGTTCATGGAAGGGGTAAAGAAGCATTGGAGCTATTGGATGCAATGGGAGCTCATGGTGTAAAGCCAAACGCAGTAACTTTCACGAGTTTGCTTTCTGCTTGCTGTCATGGAGGGCTTATTGAAGAGGGGCTCCATTTGTTTCGTGTCATGGAGAGGAAGTTTGGTGTTGTGCCTCAAATGCAGCATTATGGCTGCATTGTTGACCTTCTTGGGCGCTCTGGGCACTTGAGAGAGGCATACAAATTGATACTCGAAATGCCAATGGAACCTGATGGTGTTTTATGGAGGAGTTTGCTGAGTTCTTGTATGCTCCATGGTGATGTTGAAATGGGAGAGAGAGTGGGTAAGTTGCTTGTTGAGAGACAGGGAGGGGAGAGTTTTGATGACGAATGGTGTGTTGGAAGTGAGGACTTTGTAGCTTTGTCAAATGTTTATGCTTCTGCCGAAAGGTGGGACGATGTGGAGGCTTTGAGAGATGAAATGAAGATCAAAGGGATTGAAAACAAAGCTGGGTGTAGTTCACTACAAACTACGGGTTCTCAAGGCTTGGTGGAGGCTTTATTATAGGTATTGTACAATTAGTTTCAATTTATAAAACTGTTAATGTGGACGGT

Coding sequence (CDS)

ATGCACCTGGCCCCAAGACTGAGCTGCATCCACCACCTAAGTAACGTAAGAATTTCTTCCCTTCAACTTCTACAAATTCATGCCCAATTGATAACCAATGGCTTCAAATCTCCCTCCCCTTACGCCAAGCTAATCACCCATTTATGCAAGAAATCTTCCTCAGAATCCATCGCCCACGCCCATTTGATCTTCCGGCACCACCGATACTCTCCAAATCTCTTCCTCTTCAACACTCTCATAAGATGCGCTCCACCTCATCATTCCATCTCTATTTTCGCCACTTGGGTCTCCACCTCCCACTTCGAATTTGACGATTTCACTTTCATTTTCGTGCTTGGAGCTTGCGCGCGAGCCCCATCTGTTTCTACGTTAATGATTGGTAGGCAAATTCATACTCATATTCTTAAACGTGGGATTGTTTCGAACATTTGGGTGCAGACTACTATGATACATTTTTATTCCATTAATAAAGGTGTGGGTAGTGCGCGGAAGTTGTTTGATGAAATGTCTTTGAGAAATAGTGTTACTTGGAATGCGATGATTGCAGGGTACTGCTCACAAGGTGGTAAGGTTTCTCAGAAATATGCCCGAGATGCGTTGGAATTGTTTCGGGGGATGTTGGTTGAATCAACGAATTTTGAGGTGAAACCAACGGATACTACAATGGTTTGCATTCTTTCAGCTGCATCTCAACTAGGTATGCTTGAAACCGGTTCTTGTGTTCATGCTTATATCAAGAAGACAGTTGATTCCCCTGAAAAAGATGTGTTTATTGGCACTGGCTTGGTTAATATGTACTCAAAATGTGGGCTTCTTAACAGTGCCTCGTCAGTTTTTAAGCAGATGAAGCAGAGGAACGTTTTGACATGGACATCCATGGCGACAGGACTGGCTGTTCATGGAAGGGGTAAAGAAGCATTGGAGCTATTGGATGCAATGGGAGCTCATGGTGTAAAGCCAAACGCAGTAACTTTCACGAGTTTGCTTTCTGCTTGCTGTCATGGAGGGCTTATTGAAGAGGGGCTCCATTTGTTTCGTGTCATGGAGAGGAAGTTTGGTGTTGTGCCTCAAATGCAGCATTATGGCTGCATTGTTGACCTTCTTGGGCGCTCTGGGCACTTGAGAGAGGCATACAAATTGATACTCGAAATGCCAATGGAACCTGATGGTGTTTTATGGAGGAGTTTGCTGAGTTCTTGTATGCTCCATGGTGATGTTGAAATGGGAGAGAGAGTGGGTAAGTTGCTTGTTGAGAGACAGGGAGGGGAGAGTTTTGATGACGAATGGTGTGTTGGAAGTGAGGACTTTGTAGCTTTGTCAAATGTTTATGCTTCTGCCGAAAGGTGGGACGATGTGGAGGCTTTGAGAGATGAAATGAAGATCAAAGGGATTGAAAACAAAGCTGGGTGTAGTTCACTACAAACTACGGGTTCTCAAGGCTTGGTGGAGGCTTTATTATAG

Protein sequence

MHLAPRLSCIHHLSNVRISSLQLLQIHAQLITNGFKSPSPYAKLITHLCKKSSSESIAHAHLIFRHHRYSPNLFLFNTLIRCAPPHHSISIFATWVSTSHFEFDDFTFIFVLGACARAPSVSTLMIGRQIHTHILKRGIVSNIWVQTTMIHFYSINKGVGSARKLFDEMSLRNSVTWNAMIAGYCSQGGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASQLGMLETGSCVHAYIKKTVDSPEKDVFIGTGLVNMYSKCGLLNSASSVFKQMKQRNVLTWTSMATGLAVHGRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQHYGCIVDLLGRSGHLREAYKLILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVERQGGESFDDEWCVGSEDFVALSNVYASAERWDDVEALRDEMKIKGIENKAGCSSLQTTGSQGLVEALL*
Homology
BLAST of CSPI01G27250 vs. ExPASy Swiss-Prot
Match: Q9LJ69 (Pentatricopeptide repeat-containing protein At3g18970 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E93 PE=2 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 1.9e-121
Identity = 238/456 (52.19%), Postives = 311/456 (68.20%), Query Frame = 0

Query: 21  LQLLQIHAQLITNGFKSPSPYAKLITHLCKKSSSESIAH-AHLIFRHHRYSPNLFLFNTL 80
           +Q  QIHAQL+ NG    S + KLI H C K S+ES +  AHL+       P+ FLFNTL
Sbjct: 22  IQAKQIHAQLVINGCHDNSLFGKLIGHYCSKPSTESSSKLAHLLVFPRFGHPDKFLFNTL 81

Query: 81  IRCAPPHHSISIFATWVSTSHFEF-DDFTFIFVLGACARAPSVSTLMIGRQIHTHILKRG 140
           ++C+ P  SI IFA + S S   + ++ TF+FVLGACAR+ S S L +GR +H  + K G
Sbjct: 82  LKCSKPEDSIRIFANYASKSSLLYLNERTFVFVLGACARSASSSALRVGRIVHGMVKKLG 141

Query: 141 -IVSNIWVQTTMIHFYSINKGVGSARKLFDEMSLRNSVTWNAMIAGYCSQGGKVSQKYAR 200
            +  +  + TT++HFY+ N  +  ARK+FDEM  R SVTWNAMI GYCS   K +   AR
Sbjct: 142 FLYESELIGTTLLHFYAKNGDLRYARKVFDEMPERTSVTWNAMIGGYCSHKDKGNHN-AR 201

Query: 201 DALELFRGMLVESTNFEVKPTDTTMVCILSAASQLGMLETGSCVHAYIKKTVDSPEKDVF 260
            A+ LFR          V+PTDTTMVC+LSA SQ G+LE GS VH YI+K   +PE DVF
Sbjct: 202 KAMVLFRRF--SCCGSGVRPTDTTMVCVLSAISQTGLLEIGSLVHGYIEKLGFTPEVDVF 261

Query: 261 IGTGLVNMYSKCGLLNSASSVFKQMKQRNVLTWTSMATGLAVHGRGKEALELLDAMGAHG 320
           IGT LV+MYSKCG LN+A SVF+ MK +NV TWTSMATGLA++GRG E   LL+ M   G
Sbjct: 262 IGTALVDMYSKCGCLNNAFSVFELMKVKNVFTWTSMATGLALNGRGNETPNLLNRMAESG 321

Query: 321 VKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQHYGCIVDLLGRSGHLREA 380
           +KPN +TFTSLLSA  H GL+EEG+ LF+ M+ +FGV P ++HYGCIVDLLG++G ++EA
Sbjct: 322 IKPNEITFTSLLSAYRHIGLVEEGIELFKSMKTRFGVTPVIEHYGCIVDLLGKAGRIQEA 381

Query: 381 YKLILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVERQGGESFDDEWCVGS--ED 440
           Y+ IL MP++PD +L RSL ++C ++G+  MGE +GK L+E +     +DE   GS  ED
Sbjct: 382 YQFILAMPIKPDAILLRSLCNACSIYGETVMGEEIGKALLEIER----EDEKLSGSECED 441

Query: 441 FVALSNVYASAERWDDVEALRDEMKIKGIENKAGCS 472
           +VALSNV A   +W +VE LR EMK + I+ + G S
Sbjct: 442 YVALSNVLAHKGKWVEVEKLRKEMKERRIKTRPGYS 470

BLAST of CSPI01G27250 vs. ExPASy Swiss-Prot
Match: Q9FMA1 (Pentatricopeptide repeat-containing protein At5g56310 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E13 PE=2 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.6e-72
Identity = 167/487 (34.29%), Postives = 263/487 (54.00%), Query Frame = 0

Query: 23  LLQIHAQLITNGFKSPSPYAKLITHLCKKSSSESIAHAHLIFRHHRYSPNLFLFNTLIRC 82
           L Q H  +I  G    +         C  S++  + +A+ +F  H+  PN +L NT+IR 
Sbjct: 31  LKQSHCYMIITGLNRDNLNVAKFIEAC--SNAGHLRYAYSVFT-HQPCPNTYLHNTMIRA 90

Query: 83  -----APPHHSISIFA---TWVSTSHFEFDDFTFIFVLGACARAPSVSTLMIGRQIHTHI 142
                 P  HSI+I      W   +  + D FTF FVL    R   VS +  GRQIH  +
Sbjct: 91  LSLLDEPNAHSIAITVYRKLWALCA--KPDTFTFPFVLKIAVR---VSDVWFGRQIHGQV 150

Query: 143 LKRGIVSNIWVQTTMIHFYSINKGVGSARKLFDEMSLRNSVTWNAMIAGY---------- 202
           +  G  S++ V T +I  Y    G+G ARK+FDEM +++   WNA++AGY          
Sbjct: 151 VVFGFDSSVHVVTGLIQMYFSCGGLGDARKMFDEMLVKDVNVWNALLAGYGKVGEMDEAR 210

Query: 203 -----------------CSQGGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILS 262
                            C   G      A +A+E+F+ ML+E+    V+P + T++ +LS
Sbjct: 211 SLLEMMPCWVRNEVSWTCVISGYAKSGRASEAIEVFQRMLMEN----VEPDEVTLLAVLS 270

Query: 263 AASQLGMLETGSCVHAYIKKTVDSPEKDVFIGTGLVNMYSKCGLLNSASSVFKQMKQRNV 322
           A + LG LE G  + +Y+        + V +   +++MY+K G +  A  VF+ + +RNV
Sbjct: 271 ACADLGSLELGERICSYVDHR--GMNRAVSLNNAVIDMYAKSGNITKALDVFECVNERNV 330

Query: 323 LTWTSMATGLAVHGRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRV 382
           +TWT++  GLA HG G EAL + + M   GV+PN VTF ++LSAC H G ++ G  LF  
Sbjct: 331 VTWTTIIAGLATHGHGAEALAMFNRMVKAGVRPNDVTFIAILSACSHVGWVDLGKRLFNS 390

Query: 383 MERKFGVVPQMQHYGCIVDLLGRSGHLREAYKLILEMPMEPDGVLWRSLLSSCMLHGDVE 442
           M  K+G+ P ++HYGC++DLLGR+G LREA ++I  MP + +  +W SLL++  +H D+E
Sbjct: 391 MRSKYGIHPNIEHYGCMIDLLGRAGKLREADEVIKSMPFKANAAIWGSLLAASNVHHDLE 450

Query: 443 MGERVGKLLVERQGGESFDDEWCVGSEDFVALSNVYASAERWDDVEALRDEMKIKGIENK 475
           +GER    L++ +            S +++ L+N+Y++  RWD+   +R+ MK  G++  
Sbjct: 451 LGERALSELIKLEPN---------NSGNYMLLANLYSNLGRWDESRMMRNMMKGIGVKKM 494

BLAST of CSPI01G27250 vs. ExPASy Swiss-Prot
Match: Q9FJY7 (Pentatricopeptide repeat-containing protein At5g66520 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H61 PE=2 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 3.6e-72
Identity = 164/510 (32.16%), Postives = 273/510 (53.53%), Query Frame = 0

Query: 2   HLAPRLSCIHHLSNVRISSLQLLQIHAQLITNGFKSPS-PYAKLITHLCKKSSSESIAHA 61
           +L   +SC+   S       +L QIHA+++  G    S    K ++     +SS+ + +A
Sbjct: 13  NLYETMSCLQRCSKQE----ELKQIHARMLKTGLMQDSYAITKFLSFCISSTSSDFLPYA 72

Query: 62  HLIFRHHRYSPNLFLFNTLIR---CA-PPHHSISIFATWVSTSHFEFDDFTFIFVLGACA 121
            ++F      P+ FL+N +IR   C+  P  S+ ++   + +S    + +TF  +L AC+
Sbjct: 73  QIVFDGFD-RPDTFLWNLMIRGFSCSDEPERSLLLYQRMLCSS-APHNAYTFPSLLKACS 132

Query: 122 RAPSVSTLMIGRQIHTHILKRGIVSNIWVQTTMIHFYSINKGVGSARKLFDEMSLRNSVT 181
              ++S      QIH  I K G  ++++   ++I+ Y++      A  LFD +   + V+
Sbjct: 133 ---NLSAFEETTQIHAQITKLGYENDVYAVNSLINSYAVTGNFKLAHLLFDRIPEPDDVS 192

Query: 182 WNAMIAGYCSQG-------------------------GKVSQKYARDALELFRGMLVEST 241
           WN++I GY   G                         G V     ++AL+LF     E  
Sbjct: 193 WNSVIKGYVKAGKMDIALTLFRKMAEKNAISWTTMISGYVQADMNKEALQLFH----EMQ 252

Query: 242 NFEVKPTDTTMVCILSAASQLGMLETGSCVHAYIKKTVDSPEKDVFIGTGLVNMYSKCGL 301
           N +V+P + ++   LSA +QLG LE G  +H+Y+ KT      D  +G  L++MY+KCG 
Sbjct: 253 NSDVEPDNVSLANALSACAQLGALEQGKWIHSYLNKT--RIRMDSVLGCVLIDMYAKCGE 312

Query: 302 LNSASSVFKQMKQRNVLTWTSMATGLAVHGRGKEALELLDAMGAHGVKPNAVTFTSLLSA 361
           +  A  VFK +K+++V  WT++ +G A HG G+EA+     M   G+KPN +TFT++L+A
Sbjct: 313 MEEALEVFKNIKKKSVQAWTALISGYAYHGHGREAISKFMEMQKMGIKPNVITFTAVLTA 372

Query: 362 CCHGGLIEEGLHLFRVMERKFGVVPQMQHYGCIVDLLGRSGHLREAYKLILEMPMEPDGV 421
           C + GL+EEG  +F  MER + + P ++HYGCIVDLLGR+G L EA + I EMP++P+ V
Sbjct: 373 CSYTGLVEEGKLIFYSMERDYNLKPTIEHYGCIVDLLGRAGLLDEAKRFIQEMPLKPNAV 432

Query: 422 LWRSLLSSCMLHGDVEMGERVGKLLV---ERQGGESFDDEWCVGSEDFVALSNVYASAER 479
           +W +LL +C +H ++E+GE +G++L+      GG             +V  +N++A  ++
Sbjct: 433 IWGALLKACRIHKNIELGEEIGEILIAIDPYHGGR------------YVHKANIHAMDKK 492

BLAST of CSPI01G27250 vs. ExPASy Swiss-Prot
Match: Q9FIF7 (Putative pentatricopeptide repeat-containing protein At5g59200, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-E41 PE=3 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 6.1e-72
Identity = 166/478 (34.73%), Postives = 259/478 (54.18%), Query Frame = 0

Query: 26  IHAQLITNGFKSPSPYAKLITHLCKKSSSESIAHAHLIFRHHRYSPNLFLFNTLI----R 85
           IHA++I       +     +  +C  S+ +S+ +A+ +F +   +PN++L+  +I     
Sbjct: 48  IHAKIIRTFHDQDAFVVFELIRVC--STLDSVDYAYDVFSYVS-NPNVYLYTAMIDGFVS 107

Query: 86  CAPPHHSISIFATWVSTSHFEFDDFTFIFVLGACARAPSVSTLMIGRQIHTHILKRGIVS 145
                  +S++   +  S    D++    VL AC        L + R+IH  +LK G  S
Sbjct: 108 SGRSADGVSLYHRMIHNSVLP-DNYVITSVLKAC-------DLKVCREIHAQVLKLGFGS 167

Query: 146 NIWVQTTMIHFYSINKGVGSARKLFDEMSLRNSVTWNAMIAGYCSQG------------- 205
           +  V   M+  Y  +  + +A+K+FDEM  R+ V    MI  Y   G             
Sbjct: 168 SRSVGLKMMEIYGKSGELVNAKKMFDEMPDRDHVAATVMINCYSECGFIKEALELFQDVK 227

Query: 206 ------------GKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASQLGMLE 265
                       G V  K    ALELFR M +E+    V   + T VC+LSA S LG LE
Sbjct: 228 IKDTVCWTAMIDGLVRNKEMNKALELFREMQMEN----VSANEFTAVCVLSACSDLGALE 287

Query: 266 TGSCVHAYIKKTVDSPEKDVFIGTGLVNMYSKCGLLNSASSVFKQMKQRNVLTWTSMATG 325
            G  VH++++      E   F+G  L+NMYS+CG +N A  VF+ M+ ++V+++ +M +G
Sbjct: 288 LGRWVHSFVEN--QRMELSNFVGNALINMYSRCGDINEARRVFRVMRDKDVISYNTMISG 347

Query: 326 LAVHGRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVP 385
           LA+HG   EA+     M   G +PN VT  +LL+AC HGGL++ GL +F  M+R F V P
Sbjct: 348 LAMHGASVEAINEFRDMVNRGFRPNQVTLVALLNACSHGGLLDIGLEVFNSMKRVFNVEP 407

Query: 386 QMQHYGCIVDLLGRSGHLREAYKLILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLL 445
           Q++HYGCIVDLLGR G L EAY+ I  +P+EPD ++  +LLS+C +HG++E+GE++ K L
Sbjct: 408 QIEHYGCIVDLLGRVGRLEEAYRFIENIPIEPDHIMLGTLLSACKIHGNMELGEKIAKRL 467

Query: 446 VERQGGESFDDEWCVGSEDFVALSNVYASAERWDDVEALRDEMKIKGIENKAGCSSLQ 475
            E +  +         S  +V LSN+YAS+ +W +   +R+ M+  GIE + GCS+++
Sbjct: 468 FESENPD---------SGTYVLLSNLYASSGKWKESTEIRESMRDSGIEKEPGCSTIE 499

BLAST of CSPI01G27250 vs. ExPASy Swiss-Prot
Match: Q9FI80 (Pentatricopeptide repeat-containing protein At5g48910 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H38 PE=2 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 2.3e-71
Identity = 174/508 (34.25%), Postives = 261/508 (51.38%), Query Frame = 0

Query: 23  LLQIHAQLITNGFKSPSPYAKLITHLCKKSS--SESIAHAHLIFRHHRYSPNLFLFNTLI 82
           L QIHA  I +G    +  A  I   C  S      + +AH IF +     N F +NT+I
Sbjct: 39  LSQIHAVFIKSGQMRDTLAAAEILRFCATSDLHHRDLDYAHKIF-NQMPQRNCFSWNTII 98

Query: 83  RCAPPHH------SISIFATWVSTSHFEFDDFTFIFVLGACARAPSVSTLMIGRQIHTHI 142
           R            +I++F   +S    E + FTF  VL ACA+   +     G+QIH   
Sbjct: 99  RGFSESDEDKALIAITLFYEMMSDEFVEPNRFTFPSVLKACAKTGKIQE---GKQIHGLA 158

Query: 143 LKRGIVSNIWVQTTMIHFY----------------SINKGV------------------- 202
           LK G   + +V + ++  Y                 I K +                   
Sbjct: 159 LKYGFGGDEFVMSNLVRMYVMCGFMKDARVLFYKNIIEKDMVVMTDRRKRDGEIVLWNVM 218

Query: 203 ----------GSARKLFDEMSLRNSVTWNAMIAGYCSQGGKVSQKYARDALELFRGMLVE 262
                      +AR LFD+M  R+ V+WN MI+GY   G      + +DA+E+FR    E
Sbjct: 219 IDGYMRLGDCKAARMLFDKMRQRSVVSWNTMISGYSLNG------FFKDAVEVFR----E 278

Query: 263 STNFEVKPTDTTMVCILSAASQLGMLETGSCVHAYIKKTVDSPEKDVFIGTGLVNMYSKC 322
               +++P   T+V +L A S+LG LE G  +H Y + +      D  +G+ L++MYSKC
Sbjct: 279 MKKGDIRPNYVTLVSVLPAISRLGSLELGEWLHLYAEDS--GIRIDDVLGSALIDMYSKC 338

Query: 323 GLLNSASSVFKQMKQRNVLTWTSMATGLAVHGRGKEALELLDAMGAHGVKPNAVTFTSLL 382
           G++  A  VF+++ + NV+TW++M  G A+HG+  +A++    M   GV+P+ V + +LL
Sbjct: 339 GIIEKAIHVFERLPRENVITWSAMINGFAIHGQAGDAIDCFCKMRQAGVRPSDVAYINLL 398

Query: 383 SACCHGGLIEEGLHLFRVMERKFGVVPQMQHYGCIVDLLGRSGHLREAYKLILEMPMEPD 442
           +AC HGGL+EEG   F  M    G+ P+++HYGC+VDLLGRSG L EA + IL MP++PD
Sbjct: 399 TACSHGGLVEEGRRYFSQMVSVDGLEPRIEHYGCMVDLLGRSGLLDEAEEFILNMPIKPD 458

Query: 443 GVLWRSLLSSCMLHGDVEMGERVGKLLVERQGGESFDDEWCVGSEDFVALSNVYASAERW 478
            V+W++LL +C + G+VEMG+RV  +L++    +         S  +VALSN+YAS   W
Sbjct: 459 DVIWKALLGACRMQGNVEMGKRVANILMDMVPHD---------SGAYVALSNMYASQGNW 518

BLAST of CSPI01G27250 vs. ExPASy TrEMBL
Match: A0A0A0LZ63 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573660 PE=4 SV=1)

HSP 1 Score: 976.9 bits (2524), Expect = 3.2e-281
Identity = 482/486 (99.18%), Postives = 484/486 (99.59%), Query Frame = 0

Query: 1   MHLAPRLSCIHHLSNVRISSLQLLQIHAQLITNGFKSPSPYAKLITHLCKKSSSESIAHA 60
           MHLAPRLSCIHHLSNVRISSLQLLQIHAQLITNGFKSPSPYAKLITHLCKKSSSESIAHA
Sbjct: 1   MHLAPRLSCIHHLSNVRISSLQLLQIHAQLITNGFKSPSPYAKLITHLCKKSSSESIAHA 60

Query: 61  HLIFRHHRYSPNLFLFNTLIRCAPPHHSISIFATWVSTSHFEFDDFTFIFVLGACARAPS 120
           HLIFRHH+YSPNLFLFNTLIRCAPPHHSISIFATWVSTSHFEFDDFTFIFVLGACARAPS
Sbjct: 61  HLIFRHHQYSPNLFLFNTLIRCAPPHHSISIFATWVSTSHFEFDDFTFIFVLGACARAPS 120

Query: 121 VSTLMIGRQIHTHILKRGIVSNIWVQTTMIHFYSINKGVGSARKLFDEMSLRNSVTWNAM 180
           VSTLMIGRQIHTHILKRGIVSNIWVQTTMIHFYSINK VGSARKLFDEMSLRNSVTWNAM
Sbjct: 121 VSTLMIGRQIHTHILKRGIVSNIWVQTTMIHFYSINKDVGSARKLFDEMSLRNSVTWNAM 180

Query: 181 IAGYCSQGGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASQLGMLETGSC 240
           IAGYCSQGGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASQLGMLETGSC
Sbjct: 181 IAGYCSQGGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASQLGMLETGSC 240

Query: 241 VHAYIKKTVDSPEKDVFIGTGLVNMYSKCGLLNSASSVFKQMKQRNVLTWTSMATGLAVH 300
           VHAYIKKTVDSPEKDVFIGTGLVNMYSKCGLLNSASSVFKQMKQ+NVLTWTSMATGLAVH
Sbjct: 241 VHAYIKKTVDSPEKDVFIGTGLVNMYSKCGLLNSASSVFKQMKQKNVLTWTSMATGLAVH 300

Query: 301 GRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQH 360
           GRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQH
Sbjct: 301 GRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQH 360

Query: 361 YGCIVDLLGRSGHLREAYKLILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVERQ 420
           YGCIVDLLGRSGHLREAYKLILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVERQ
Sbjct: 361 YGCIVDLLGRSGHLREAYKLILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVERQ 420

Query: 421 GGESFDDEWCVGSEDFVALSNVYASAERWDDVEALRDEMKIKGIENKAGCSSLQTTGSQG 480
           GGESFDDEWCVGSEDFVALSNVYAS ERWDDVEALRDEMKIKGIENKAGCSSLQTTGSQG
Sbjct: 421 GGESFDDEWCVGSEDFVALSNVYASVERWDDVEALRDEMKIKGIENKAGCSSLQTTGSQG 480

Query: 481 LVEALL 487
           LVEALL
Sbjct: 481 LVEALL 486

BLAST of CSPI01G27250 vs. ExPASy TrEMBL
Match: A0A5D3CFP0 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003250 PE=4 SV=1)

HSP 1 Score: 939.1 bits (2426), Expect = 7.3e-270
Identity = 462/486 (95.06%), Postives = 474/486 (97.53%), Query Frame = 0

Query: 1   MHLAPRLSCIHHLSNVRISSLQLLQIHAQLITNGFKSPSPYAKLITHLCKKSSSESIAHA 60
           MHLAPRLSCI+HLSN+RISSLQLLQIHAQ ITNGFKSPSPYAKLITHLCKKSSSESIAHA
Sbjct: 9   MHLAPRLSCINHLSNIRISSLQLLQIHAQFITNGFKSPSPYAKLITHLCKKSSSESIAHA 68

Query: 61  HLIFRHHRYSPNLFLFNTLIRCAPPHHSISIFATWVSTSHFEFDDFTFIFVLGACARAPS 120
           HLIFRHH++SPNLFLFNTLIRCAPP +SISIFA WVST HFEFDDFTFIFVLGACARAPS
Sbjct: 69  HLIFRHHQHSPNLFLFNTLIRCAPPQYSISIFANWVSTPHFEFDDFTFIFVLGACARAPS 128

Query: 121 VSTLMIGRQIHTHILKRGIVSNIWVQTTMIHFYSINKGVGSARKLFDEMSLRNSVTWNAM 180
           VSTLMIGRQIHTHILKRGIVSNIW QTTMIHFYS NK VGSARK+FDEMS+RNSVTWNAM
Sbjct: 129 VSTLMIGRQIHTHILKRGIVSNIWAQTTMIHFYSTNKDVGSARKVFDEMSVRNSVTWNAM 188

Query: 181 IAGYCSQGGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASQLGMLETGSC 240
           IAGYCSQ GKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAAS LGMLETG C
Sbjct: 189 IAGYCSQSGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASHLGMLETGVC 248

Query: 241 VHAYIKKTVDSPEKDVFIGTGLVNMYSKCGLLNSASSVFKQMKQRNVLTWTSMATGLAVH 300
           VHAYIKKT+DSPEKDVFIGTGLVNMYSKCGLL+SASSVFKQMKQRNVLTWTSMATGLAVH
Sbjct: 249 VHAYIKKTIDSPEKDVFIGTGLVNMYSKCGLLSSASSVFKQMKQRNVLTWTSMATGLAVH 308

Query: 301 GRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQH 360
           GRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQH
Sbjct: 309 GRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQH 368

Query: 361 YGCIVDLLGRSGHLREAYKLILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVERQ 420
           YGCIVDLLGRSGHLREAY+LILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVERQ
Sbjct: 369 YGCIVDLLGRSGHLREAYELILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVERQ 428

Query: 421 GGESFDDEWCVGSEDFVALSNVYASAERWDDVEALRDEMKIKGIENKAGCSSLQTTGSQG 480
           GGESFDDEWCVGSEDFVALSNVYASAERWDDVEALR+EMKIKGIENKAG SS+QTTGSQG
Sbjct: 429 GGESFDDEWCVGSEDFVALSNVYASAERWDDVEALREEMKIKGIENKAGFSSVQTTGSQG 488

Query: 481 LVEALL 487
           LVE LL
Sbjct: 489 LVETLL 494

BLAST of CSPI01G27250 vs. ExPASy TrEMBL
Match: A0A1S3BPA5 (pentatricopeptide repeat-containing protein At3g18970 OS=Cucumis melo OX=3656 GN=LOC103492230 PE=4 SV=1)

HSP 1 Score: 939.1 bits (2426), Expect = 7.3e-270
Identity = 462/486 (95.06%), Postives = 474/486 (97.53%), Query Frame = 0

Query: 1   MHLAPRLSCIHHLSNVRISSLQLLQIHAQLITNGFKSPSPYAKLITHLCKKSSSESIAHA 60
           MHLAPRLSCI+HLSN+RISSLQLLQIHAQ ITNGFKSPSPYAKLITHLCKKSSSESIAHA
Sbjct: 9   MHLAPRLSCINHLSNIRISSLQLLQIHAQFITNGFKSPSPYAKLITHLCKKSSSESIAHA 68

Query: 61  HLIFRHHRYSPNLFLFNTLIRCAPPHHSISIFATWVSTSHFEFDDFTFIFVLGACARAPS 120
           HLIFRHH++SPNLFLFNTLIRCAPP +SISIFA WVST HFEFDDFTFIFVLGACARAPS
Sbjct: 69  HLIFRHHQHSPNLFLFNTLIRCAPPQYSISIFANWVSTPHFEFDDFTFIFVLGACARAPS 128

Query: 121 VSTLMIGRQIHTHILKRGIVSNIWVQTTMIHFYSINKGVGSARKLFDEMSLRNSVTWNAM 180
           VSTLMIGRQIHTHILKRGIVSNIW QTTMIHFYS NK VGSARK+FDEMS+RNSVTWNAM
Sbjct: 129 VSTLMIGRQIHTHILKRGIVSNIWAQTTMIHFYSTNKDVGSARKVFDEMSVRNSVTWNAM 188

Query: 181 IAGYCSQGGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASQLGMLETGSC 240
           IAGYCSQ GKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAAS LGMLETG C
Sbjct: 189 IAGYCSQSGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASHLGMLETGVC 248

Query: 241 VHAYIKKTVDSPEKDVFIGTGLVNMYSKCGLLNSASSVFKQMKQRNVLTWTSMATGLAVH 300
           VHAYIKKT+DSPEKDVFIGTGLVNMYSKCGLL+SASSVFKQMKQRNVLTWTSMATGLAVH
Sbjct: 249 VHAYIKKTIDSPEKDVFIGTGLVNMYSKCGLLSSASSVFKQMKQRNVLTWTSMATGLAVH 308

Query: 301 GRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQH 360
           GRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQH
Sbjct: 309 GRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQH 368

Query: 361 YGCIVDLLGRSGHLREAYKLILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVERQ 420
           YGCIVDLLGRSGHLREAY+LILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVERQ
Sbjct: 369 YGCIVDLLGRSGHLREAYELILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVERQ 428

Query: 421 GGESFDDEWCVGSEDFVALSNVYASAERWDDVEALRDEMKIKGIENKAGCSSLQTTGSQG 480
           GGESFDDEWCVGSEDFVALSNVYASAERWDDVEALR+EMKIKGIENKAG SS+QTTGSQG
Sbjct: 429 GGESFDDEWCVGSEDFVALSNVYASAERWDDVEALREEMKIKGIENKAGFSSVQTTGSQG 488

Query: 481 LVEALL 487
           LVE LL
Sbjct: 489 LVETLL 494

BLAST of CSPI01G27250 vs. ExPASy TrEMBL
Match: A0A6J1JKN4 (pentatricopeptide repeat-containing protein At3g18970 OS=Cucurbita maxima OX=3661 GN=LOC111485442 PE=4 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 1.2e-224
Identity = 385/478 (80.54%), Postives = 428/478 (89.54%), Query Frame = 0

Query: 1   MHLAPRLSCIHHLSNVRISS-LQLLQIHAQLITNGFKSPSPYAKLITHLCKKSSSESIAH 60
           MHL+PRLSCIHHL+N+  SS LQL QI AQLITN FKSPSPYAKLI H C K S ++ A+
Sbjct: 1   MHLSPRLSCIHHLNNILNSSLLQLKQIQAQLITNAFKSPSPYAKLIAHFCNKHSPQATAY 60

Query: 61  AHLIFRHHRYSPNLFLFNTLIRCAPPHHSISIFATWVSTSHFEFDDFTFIFVLGACARAP 120
           AHLI  HHR+  NLFLFNTLIRCAPP HSISIFA  +ST+HFEFDDFTFIF+LGACARAP
Sbjct: 61  AHLISLHHRHPTNLFLFNTLIRCAPPQHSISIFANSLSTAHFEFDDFTFIFLLGACARAP 120

Query: 121 SVSTLMIGRQIHTHILKRGIVSNIWVQTTMIHFYSINKGVGSARKLFDEMSLRNSVTWNA 180
           S  TL  G+QIHTH+LKRG+VSNIWVQTTMIHFY+INK VG+ARK+FDEMS+RN+VTWNA
Sbjct: 121 SPPTLTTGKQIHTHVLKRGVVSNIWVQTTMIHFYAINKDVGAARKVFDEMSVRNNVTWNA 180

Query: 181 MIAGYCSQGGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASQLGMLETGS 240
           MIAGYCSQ G+V+QKY R+ALELFRGMLVESTN +V PTDTTMVC+LSAASQLG+LETG 
Sbjct: 181 MIAGYCSQSGRVAQKYGREALELFRGMLVESTNSDVNPTDTTMVCLLSAASQLGVLETGV 240

Query: 241 CVHAYIKKTVDSPEKDVFIGTGLVNMYSKCGLLNSASSVFKQMKQRNVLTWTSMATGLAV 300
           CVHAYI+KT DSPE DVFIGTGLVNMYSKCG LNSASSVFKQMKQRNVLTWT+MATGLA+
Sbjct: 241 CVHAYIEKTTDSPENDVFIGTGLVNMYSKCGCLNSASSVFKQMKQRNVLTWTAMATGLAI 300

Query: 301 HGRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQ 360
           HG+GKEALELL+AMG HGVKPNAVTFTSLLS CCHGGLIEEGLHLF VMERKFGVVPQMQ
Sbjct: 301 HGKGKEALELLEAMGGHGVKPNAVTFTSLLSGCCHGGLIEEGLHLFDVMERKFGVVPQMQ 360

Query: 361 HYGCIVDLLGRSGHLREAYKLILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVER 420
           HYGCIVDLLGR GHL+EAY+LIL MP+ PDGVLWRSL+SSCM+H DVEMGE+VGK LVE 
Sbjct: 361 HYGCIVDLLGRCGHLKEAYELILGMPVAPDGVLWRSLMSSCMVHCDVEMGEKVGKFLVES 420

Query: 421 QGGESFDDEWCVGSEDFVALSNVYASAERWDDVEALRDEMKIKGIENKAGCSSLQTTG 478
             G    DEWC GSEDFVALSNVYASA+RW++V+A+R+EMKIKGI+NKAG SS+QT G
Sbjct: 421 VVG----DEWCDGSEDFVALSNVYASAQRWENVKAVREEMKIKGIQNKAGYSSVQTRG 474

BLAST of CSPI01G27250 vs. ExPASy TrEMBL
Match: A0A6J1HCU6 (pentatricopeptide repeat-containing protein At3g18970 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461664 PE=4 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 4.8e-221
Identity = 381/478 (79.71%), Postives = 423/478 (88.49%), Query Frame = 0

Query: 1   MHLAPRLSCIHHLSNVRISS-LQLLQIHAQLITNGFKSPSPYAKLITHLCKKSSSESIAH 60
           MHL+PRLSCIH L+N+  SS LQL QIHAQLITN FKSPSPYAKLI H C K S E+ A+
Sbjct: 1   MHLSPRLSCIHLLNNILNSSLLQLKQIHAQLITNAFKSPSPYAKLIAHFCNKHSPEATAY 60

Query: 61  AHLIFRHHRYSPNLFLFNTLIRCAPPHHSISIFATWVSTSHFEFDDFTFIFVLGACARAP 120
           AH I  HHR+  NLFLFNTLIRCAPP HSISIFA  VST+HF+FDDFTFIF+LGACARAP
Sbjct: 61  AHSISLHHRHPTNLFLFNTLIRCAPPQHSISIFANSVSTAHFQFDDFTFIFLLGACARAP 120

Query: 121 SVSTLMIGRQIHTHILKRGIVSNIWVQTTMIHFYSINKGVGSARKLFDEMSLRNSVTWNA 180
           S  TL  G+QIHTHILKRG+VSNIWVQTTMIHFY+INK VG+ARK+FDEM +RN+VTWNA
Sbjct: 121 SPPTLTTGKQIHTHILKRGVVSNIWVQTTMIHFYAINKDVGAARKVFDEMPVRNNVTWNA 180

Query: 181 MIAGYCSQGGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASQLGMLETGS 240
           MIAGYCSQ G+V+ KY  +AL+LFR MLVESTN +V PTDTTMVC+LSAASQLG+LETG 
Sbjct: 181 MIAGYCSQSGRVAHKYGLEALDLFRAMLVESTNSDVNPTDTTMVCLLSAASQLGVLETGV 240

Query: 241 CVHAYIKKTVDSPEKDVFIGTGLVNMYSKCGLLNSASSVFKQMKQRNVLTWTSMATGLAV 300
           CVHAYI+KT+DSPE DVFIGTGLVNMYSKCG LNSAS VFKQMKQRNVLTWT+MATGLA+
Sbjct: 241 CVHAYIEKTIDSPENDVFIGTGLVNMYSKCGCLNSASLVFKQMKQRNVLTWTAMATGLAI 300

Query: 301 HGRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQ 360
           HG+GKEALELL+AMG HGVKPNAVTFTSLLS CCHGGLIEEGLHLF VMERKFGVVPQMQ
Sbjct: 301 HGKGKEALELLEAMGGHGVKPNAVTFTSLLSGCCHGGLIEEGLHLFDVMERKFGVVPQMQ 360

Query: 361 HYGCIVDLLGRSGHLREAYKLILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVER 420
           HYGCIVDLLGR GHL+EAY+LIL MPM PDGVLWRSL+SSCM+H DVEMGE+VGK LVE 
Sbjct: 361 HYGCIVDLLGRCGHLKEAYELILGMPMAPDGVLWRSLMSSCMVHCDVEMGEKVGKFLVES 420

Query: 421 QGGESFDDEWCVGSEDFVALSNVYASAERWDDVEALRDEMKIKGIENKAGCSSLQTTG 478
             G    DEWC GSEDFVALSNVYASA+RW++V+A+R+EMKIKGI+NKAG SS+QT G
Sbjct: 421 VVG----DEWCDGSEDFVALSNVYASAQRWENVKAVREEMKIKGIQNKAGYSSVQTGG 474

BLAST of CSPI01G27250 vs. NCBI nr
Match: XP_011659935.1 (pentatricopeptide repeat-containing protein At3g18970 [Cucumis sativus])

HSP 1 Score: 976.9 bits (2524), Expect = 6.6e-281
Identity = 482/486 (99.18%), Postives = 484/486 (99.59%), Query Frame = 0

Query: 1   MHLAPRLSCIHHLSNVRISSLQLLQIHAQLITNGFKSPSPYAKLITHLCKKSSSESIAHA 60
           MHLAPRLSCIHHLSNVRISSLQLLQIHAQLITNGFKSPSPYAKLITHLCKKSSSESIAHA
Sbjct: 1   MHLAPRLSCIHHLSNVRISSLQLLQIHAQLITNGFKSPSPYAKLITHLCKKSSSESIAHA 60

Query: 61  HLIFRHHRYSPNLFLFNTLIRCAPPHHSISIFATWVSTSHFEFDDFTFIFVLGACARAPS 120
           HLIFRHH+YSPNLFLFNTLIRCAPPHHSISIFATWVSTSHFEFDDFTFIFVLGACARAPS
Sbjct: 61  HLIFRHHQYSPNLFLFNTLIRCAPPHHSISIFATWVSTSHFEFDDFTFIFVLGACARAPS 120

Query: 121 VSTLMIGRQIHTHILKRGIVSNIWVQTTMIHFYSINKGVGSARKLFDEMSLRNSVTWNAM 180
           VSTLMIGRQIHTHILKRGIVSNIWVQTTMIHFYSINK VGSARKLFDEMSLRNSVTWNAM
Sbjct: 121 VSTLMIGRQIHTHILKRGIVSNIWVQTTMIHFYSINKDVGSARKLFDEMSLRNSVTWNAM 180

Query: 181 IAGYCSQGGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASQLGMLETGSC 240
           IAGYCSQGGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASQLGMLETGSC
Sbjct: 181 IAGYCSQGGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASQLGMLETGSC 240

Query: 241 VHAYIKKTVDSPEKDVFIGTGLVNMYSKCGLLNSASSVFKQMKQRNVLTWTSMATGLAVH 300
           VHAYIKKTVDSPEKDVFIGTGLVNMYSKCGLLNSASSVFKQMKQ+NVLTWTSMATGLAVH
Sbjct: 241 VHAYIKKTVDSPEKDVFIGTGLVNMYSKCGLLNSASSVFKQMKQKNVLTWTSMATGLAVH 300

Query: 301 GRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQH 360
           GRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQH
Sbjct: 301 GRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQH 360

Query: 361 YGCIVDLLGRSGHLREAYKLILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVERQ 420
           YGCIVDLLGRSGHLREAYKLILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVERQ
Sbjct: 361 YGCIVDLLGRSGHLREAYKLILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVERQ 420

Query: 421 GGESFDDEWCVGSEDFVALSNVYASAERWDDVEALRDEMKIKGIENKAGCSSLQTTGSQG 480
           GGESFDDEWCVGSEDFVALSNVYAS ERWDDVEALRDEMKIKGIENKAGCSSLQTTGSQG
Sbjct: 421 GGESFDDEWCVGSEDFVALSNVYASVERWDDVEALRDEMKIKGIENKAGCSSLQTTGSQG 480

Query: 481 LVEALL 487
           LVEALL
Sbjct: 481 LVEALL 486

BLAST of CSPI01G27250 vs. NCBI nr
Match: XP_008450741.1 (PREDICTED: pentatricopeptide repeat-containing protein At3g18970 [Cucumis melo] >TYK10180.1 pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 939.1 bits (2426), Expect = 1.5e-269
Identity = 462/486 (95.06%), Postives = 474/486 (97.53%), Query Frame = 0

Query: 1   MHLAPRLSCIHHLSNVRISSLQLLQIHAQLITNGFKSPSPYAKLITHLCKKSSSESIAHA 60
           MHLAPRLSCI+HLSN+RISSLQLLQIHAQ ITNGFKSPSPYAKLITHLCKKSSSESIAHA
Sbjct: 9   MHLAPRLSCINHLSNIRISSLQLLQIHAQFITNGFKSPSPYAKLITHLCKKSSSESIAHA 68

Query: 61  HLIFRHHRYSPNLFLFNTLIRCAPPHHSISIFATWVSTSHFEFDDFTFIFVLGACARAPS 120
           HLIFRHH++SPNLFLFNTLIRCAPP +SISIFA WVST HFEFDDFTFIFVLGACARAPS
Sbjct: 69  HLIFRHHQHSPNLFLFNTLIRCAPPQYSISIFANWVSTPHFEFDDFTFIFVLGACARAPS 128

Query: 121 VSTLMIGRQIHTHILKRGIVSNIWVQTTMIHFYSINKGVGSARKLFDEMSLRNSVTWNAM 180
           VSTLMIGRQIHTHILKRGIVSNIW QTTMIHFYS NK VGSARK+FDEMS+RNSVTWNAM
Sbjct: 129 VSTLMIGRQIHTHILKRGIVSNIWAQTTMIHFYSTNKDVGSARKVFDEMSVRNSVTWNAM 188

Query: 181 IAGYCSQGGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASQLGMLETGSC 240
           IAGYCSQ GKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAAS LGMLETG C
Sbjct: 189 IAGYCSQSGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASHLGMLETGVC 248

Query: 241 VHAYIKKTVDSPEKDVFIGTGLVNMYSKCGLLNSASSVFKQMKQRNVLTWTSMATGLAVH 300
           VHAYIKKT+DSPEKDVFIGTGLVNMYSKCGLL+SASSVFKQMKQRNVLTWTSMATGLAVH
Sbjct: 249 VHAYIKKTIDSPEKDVFIGTGLVNMYSKCGLLSSASSVFKQMKQRNVLTWTSMATGLAVH 308

Query: 301 GRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQH 360
           GRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQH
Sbjct: 309 GRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQH 368

Query: 361 YGCIVDLLGRSGHLREAYKLILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVERQ 420
           YGCIVDLLGRSGHLREAY+LILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVERQ
Sbjct: 369 YGCIVDLLGRSGHLREAYELILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVERQ 428

Query: 421 GGESFDDEWCVGSEDFVALSNVYASAERWDDVEALRDEMKIKGIENKAGCSSLQTTGSQG 480
           GGESFDDEWCVGSEDFVALSNVYASAERWDDVEALR+EMKIKGIENKAG SS+QTTGSQG
Sbjct: 429 GGESFDDEWCVGSEDFVALSNVYASAERWDDVEALREEMKIKGIENKAGFSSVQTTGSQG 488

Query: 481 LVEALL 487
           LVE LL
Sbjct: 489 LVETLL 494

BLAST of CSPI01G27250 vs. NCBI nr
Match: XP_038877553.1 (pentatricopeptide repeat-containing protein At3g18970 [Benincasa hispida] >XP_038877554.1 pentatricopeptide repeat-containing protein At3g18970 [Benincasa hispida])

HSP 1 Score: 863.6 bits (2230), Expect = 8.1e-247
Identity = 427/485 (88.04%), Postives = 457/485 (94.23%), Query Frame = 0

Query: 1   MHLAPRLSCIHHLSNVRISSLQLLQIHAQLITNGFKSPSPYAKLITHLCKKSSSESIAHA 60
           MHLAPR+ CIHHL+N+R SSLQL QIHAQLIT+GFK PSPYAKLITH C KSS E+IA+A
Sbjct: 30  MHLAPRVRCIHHLNNIRNSSLQLKQIHAQLITSGFKPPSPYAKLITHFCNKSSPEAIAYA 89

Query: 61  HLIFRHHRYSPNLFLFNTLIRCAPPHHSISIFATWVSTSHFEFDDFTFIFVLGACARAPS 120
           HLIFRH+++ PNLFLFNTLIRCAPP HSISIFA+WVS  HFEFD+FTFIFVLGACARAPS
Sbjct: 90  HLIFRHYQHPPNLFLFNTLIRCAPPQHSISIFASWVSNPHFEFDNFTFIFVLGACARAPS 149

Query: 121 VSTLMIGRQIHTHILKRGIVSNIWVQTTMIHFYSINKGVGSARKLFDEMSLRNSVTWNAM 180
           VSTLMIG+QIHT ILKRGIVSNI VQTTMIHFY+INK VG+ARK+FDEMS+RNSVTWNAM
Sbjct: 150 VSTLMIGKQIHTLILKRGIVSNITVQTTMIHFYAINKNVGTARKVFDEMSVRNSVTWNAM 209

Query: 181 IAGYCSQGGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASQLGMLETGSC 240
           IAGYCSQ    + KYARDALELFRGMLVESTN EVKPTDTTMVC+LSAASQLGMLETG+C
Sbjct: 210 IAGYCSQ---CAHKYARDALELFRGMLVESTNSEVKPTDTTMVCLLSAASQLGMLETGAC 269

Query: 241 VHAYIKKTVDSPEKDVFIGTGLVNMYSKCGLLNSASSVFKQMKQRNVLTWTSMATGLAVH 300
           VHAYI+KT+DSPE D+FIGTGLVNMYSKCG LNSASSVFKQMKQRNVLTWT+MATGLAVH
Sbjct: 270 VHAYIEKTIDSPENDLFIGTGLVNMYSKCGCLNSASSVFKQMKQRNVLTWTAMATGLAVH 329

Query: 301 GRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQH 360
           GRGKEALELLDAMG HGVKPNAVTFTSLLSACCHGGLIEEGLHLF VMERKFGVVPQMQH
Sbjct: 330 GRGKEALELLDAMGTHGVKPNAVTFTSLLSACCHGGLIEEGLHLFHVMERKFGVVPQMQH 389

Query: 361 YGCIVDLLGRSGHLREAYKLILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVERQ 420
           YGCIVDLLGRSGHLREAY+LIL MPME DGVLWRSLLSSCM+HG+VEMGERVGKLLVERQ
Sbjct: 390 YGCIVDLLGRSGHLREAYELILGMPMESDGVLWRSLLSSCMVHGNVEMGERVGKLLVERQ 449

Query: 421 GGESFDDEWCVGSEDFVALSNVYASAERWDDVEALRDEMKIKGIENKAGCSSLQTTGSQG 480
           GGESFDDE CVGSEDFVALSNVYASAERW+DVEA+R+EMKIKGIENKAGCSS+QTTGSQG
Sbjct: 450 GGESFDDERCVGSEDFVALSNVYASAERWEDVEAVREEMKIKGIENKAGCSSVQTTGSQG 509

Query: 481 LVEAL 486
           L EAL
Sbjct: 510 L-EAL 510

BLAST of CSPI01G27250 vs. NCBI nr
Match: XP_022988094.1 (pentatricopeptide repeat-containing protein At3g18970 [Cucurbita maxima])

HSP 1 Score: 788.9 bits (2036), Expect = 2.5e-224
Identity = 385/478 (80.54%), Postives = 428/478 (89.54%), Query Frame = 0

Query: 1   MHLAPRLSCIHHLSNVRISS-LQLLQIHAQLITNGFKSPSPYAKLITHLCKKSSSESIAH 60
           MHL+PRLSCIHHL+N+  SS LQL QI AQLITN FKSPSPYAKLI H C K S ++ A+
Sbjct: 1   MHLSPRLSCIHHLNNILNSSLLQLKQIQAQLITNAFKSPSPYAKLIAHFCNKHSPQATAY 60

Query: 61  AHLIFRHHRYSPNLFLFNTLIRCAPPHHSISIFATWVSTSHFEFDDFTFIFVLGACARAP 120
           AHLI  HHR+  NLFLFNTLIRCAPP HSISIFA  +ST+HFEFDDFTFIF+LGACARAP
Sbjct: 61  AHLISLHHRHPTNLFLFNTLIRCAPPQHSISIFANSLSTAHFEFDDFTFIFLLGACARAP 120

Query: 121 SVSTLMIGRQIHTHILKRGIVSNIWVQTTMIHFYSINKGVGSARKLFDEMSLRNSVTWNA 180
           S  TL  G+QIHTH+LKRG+VSNIWVQTTMIHFY+INK VG+ARK+FDEMS+RN+VTWNA
Sbjct: 121 SPPTLTTGKQIHTHVLKRGVVSNIWVQTTMIHFYAINKDVGAARKVFDEMSVRNNVTWNA 180

Query: 181 MIAGYCSQGGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASQLGMLETGS 240
           MIAGYCSQ G+V+QKY R+ALELFRGMLVESTN +V PTDTTMVC+LSAASQLG+LETG 
Sbjct: 181 MIAGYCSQSGRVAQKYGREALELFRGMLVESTNSDVNPTDTTMVCLLSAASQLGVLETGV 240

Query: 241 CVHAYIKKTVDSPEKDVFIGTGLVNMYSKCGLLNSASSVFKQMKQRNVLTWTSMATGLAV 300
           CVHAYI+KT DSPE DVFIGTGLVNMYSKCG LNSASSVFKQMKQRNVLTWT+MATGLA+
Sbjct: 241 CVHAYIEKTTDSPENDVFIGTGLVNMYSKCGCLNSASSVFKQMKQRNVLTWTAMATGLAI 300

Query: 301 HGRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQ 360
           HG+GKEALELL+AMG HGVKPNAVTFTSLLS CCHGGLIEEGLHLF VMERKFGVVPQMQ
Sbjct: 301 HGKGKEALELLEAMGGHGVKPNAVTFTSLLSGCCHGGLIEEGLHLFDVMERKFGVVPQMQ 360

Query: 361 HYGCIVDLLGRSGHLREAYKLILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVER 420
           HYGCIVDLLGR GHL+EAY+LIL MP+ PDGVLWRSL+SSCM+H DVEMGE+VGK LVE 
Sbjct: 361 HYGCIVDLLGRCGHLKEAYELILGMPVAPDGVLWRSLMSSCMVHCDVEMGEKVGKFLVES 420

Query: 421 QGGESFDDEWCVGSEDFVALSNVYASAERWDDVEALRDEMKIKGIENKAGCSSLQTTG 478
             G    DEWC GSEDFVALSNVYASA+RW++V+A+R+EMKIKGI+NKAG SS+QT G
Sbjct: 421 VVG----DEWCDGSEDFVALSNVYASAQRWENVKAVREEMKIKGIQNKAGYSSVQTRG 474

BLAST of CSPI01G27250 vs. NCBI nr
Match: XP_023515866.1 (pentatricopeptide repeat-containing protein At3g18970 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 786.2 bits (2029), Expect = 1.6e-223
Identity = 384/478 (80.33%), Postives = 425/478 (88.91%), Query Frame = 0

Query: 1   MHLAPRLSCIHHLSNVRISS-LQLLQIHAQLITNGFKSPSPYAKLITHLCKKSSSESIAH 60
           M L+PRLSCIHHL+N+  SS LQL QIHAQLITN FKSP PYAKLI H C   S E+ A+
Sbjct: 1   MRLSPRLSCIHHLNNILSSSLLQLKQIHAQLITNAFKSPIPYAKLIAHFCNNHSPEATAY 60

Query: 61  AHLIFRHHRYSPNLFLFNTLIRCAPPHHSISIFATWVSTSHFEFDDFTFIFVLGACARAP 120
           AHLI  HHR+  NLFLFNTLIRCAPP HSISIFA  VST+HFEFDDFTFIF+LGACARAP
Sbjct: 61  AHLISLHHRHPTNLFLFNTLIRCAPPQHSISIFANSVSTAHFEFDDFTFIFLLGACARAP 120

Query: 121 SVSTLMIGRQIHTHILKRGIVSNIWVQTTMIHFYSINKGVGSARKLFDEMSLRNSVTWNA 180
           S  TL  G+QIHTHILKRG+VSNIWVQTTMIHFY+INK VG+ARK+FDEMS+RN+VTWNA
Sbjct: 121 SPPTLTTGKQIHTHILKRGVVSNIWVQTTMIHFYAINKDVGAARKVFDEMSVRNNVTWNA 180

Query: 181 MIAGYCSQGGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASQLGMLETGS 240
           MIAGYCSQ G+V+QKY R+ALELFRGMLVESTN +V PTDTTMVC+LSAASQLG+LETG 
Sbjct: 181 MIAGYCSQSGRVAQKYGREALELFRGMLVESTNSDVNPTDTTMVCLLSAASQLGVLETGV 240

Query: 241 CVHAYIKKTVDSPEKDVFIGTGLVNMYSKCGLLNSASSVFKQMKQRNVLTWTSMATGLAV 300
           CVHAYI+KT+DSPE DVFIGTGLVNMYSKCG LNSASSVFKQMKQRNVLTWT+MATGLA+
Sbjct: 241 CVHAYIEKTIDSPENDVFIGTGLVNMYSKCGCLNSASSVFKQMKQRNVLTWTAMATGLAI 300

Query: 301 HGRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQ 360
           HG+GKEALELL+AMG HGVKPNAVTFTSLLS CCHGGLIEEGLHLF VMERKFGVVPQMQ
Sbjct: 301 HGKGKEALELLEAMGGHGVKPNAVTFTSLLSGCCHGGLIEEGLHLFDVMERKFGVVPQMQ 360

Query: 361 HYGCIVDLLGRSGHLREAYKLILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVER 420
           HYGCIVDLLGR GHL+EAY++IL MPM PDGVLWR L+SSCM+H DVEMGE+VGK LVE 
Sbjct: 361 HYGCIVDLLGRCGHLKEAYEMILGMPMAPDGVLWRGLMSSCMVHCDVEMGEKVGKFLVES 420

Query: 421 QGGESFDDEWCVGSEDFVALSNVYASAERWDDVEALRDEMKIKGIENKAGCSSLQTTG 478
             G    DEWC GSEDFVALSNVYASA+RW++V+A+R+EMKIKGI+NK G SS+QT G
Sbjct: 421 VVG----DEWCDGSEDFVALSNVYASAQRWENVKAVREEMKIKGIQNKRGYSSVQTRG 474

BLAST of CSPI01G27250 vs. TAIR 10
Match: AT3G18970.1 (mitochondrial editing factor 20 )

HSP 1 Score: 437.6 bits (1124), Expect = 1.3e-122
Identity = 238/456 (52.19%), Postives = 311/456 (68.20%), Query Frame = 0

Query: 21  LQLLQIHAQLITNGFKSPSPYAKLITHLCKKSSSESIAH-AHLIFRHHRYSPNLFLFNTL 80
           +Q  QIHAQL+ NG    S + KLI H C K S+ES +  AHL+       P+ FLFNTL
Sbjct: 22  IQAKQIHAQLVINGCHDNSLFGKLIGHYCSKPSTESSSKLAHLLVFPRFGHPDKFLFNTL 81

Query: 81  IRCAPPHHSISIFATWVSTSHFEF-DDFTFIFVLGACARAPSVSTLMIGRQIHTHILKRG 140
           ++C+ P  SI IFA + S S   + ++ TF+FVLGACAR+ S S L +GR +H  + K G
Sbjct: 82  LKCSKPEDSIRIFANYASKSSLLYLNERTFVFVLGACARSASSSALRVGRIVHGMVKKLG 141

Query: 141 -IVSNIWVQTTMIHFYSINKGVGSARKLFDEMSLRNSVTWNAMIAGYCSQGGKVSQKYAR 200
            +  +  + TT++HFY+ N  +  ARK+FDEM  R SVTWNAMI GYCS   K +   AR
Sbjct: 142 FLYESELIGTTLLHFYAKNGDLRYARKVFDEMPERTSVTWNAMIGGYCSHKDKGNHN-AR 201

Query: 201 DALELFRGMLVESTNFEVKPTDTTMVCILSAASQLGMLETGSCVHAYIKKTVDSPEKDVF 260
            A+ LFR          V+PTDTTMVC+LSA SQ G+LE GS VH YI+K   +PE DVF
Sbjct: 202 KAMVLFRRF--SCCGSGVRPTDTTMVCVLSAISQTGLLEIGSLVHGYIEKLGFTPEVDVF 261

Query: 261 IGTGLVNMYSKCGLLNSASSVFKQMKQRNVLTWTSMATGLAVHGRGKEALELLDAMGAHG 320
           IGT LV+MYSKCG LN+A SVF+ MK +NV TWTSMATGLA++GRG E   LL+ M   G
Sbjct: 262 IGTALVDMYSKCGCLNNAFSVFELMKVKNVFTWTSMATGLALNGRGNETPNLLNRMAESG 321

Query: 321 VKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVPQMQHYGCIVDLLGRSGHLREA 380
           +KPN +TFTSLLSA  H GL+EEG+ LF+ M+ +FGV P ++HYGCIVDLLG++G ++EA
Sbjct: 322 IKPNEITFTSLLSAYRHIGLVEEGIELFKSMKTRFGVTPVIEHYGCIVDLLGKAGRIQEA 381

Query: 381 YKLILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLLVERQGGESFDDEWCVGS--ED 440
           Y+ IL MP++PD +L RSL ++C ++G+  MGE +GK L+E +     +DE   GS  ED
Sbjct: 382 YQFILAMPIKPDAILLRSLCNACSIYGETVMGEEIGKALLEIER----EDEKLSGSECED 441

Query: 441 FVALSNVYASAERWDDVEALRDEMKIKGIENKAGCS 472
           +VALSNV A   +W +VE LR EMK + I+ + G S
Sbjct: 442 YVALSNVLAHKGKWVEVEKLRKEMKERRIKTRPGYS 470

BLAST of CSPI01G27250 vs. TAIR 10
Match: AT5G56310.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 275.0 bits (702), Expect = 1.1e-73
Identity = 167/487 (34.29%), Postives = 263/487 (54.00%), Query Frame = 0

Query: 23  LLQIHAQLITNGFKSPSPYAKLITHLCKKSSSESIAHAHLIFRHHRYSPNLFLFNTLIRC 82
           L Q H  +I  G    +         C  S++  + +A+ +F  H+  PN +L NT+IR 
Sbjct: 31  LKQSHCYMIITGLNRDNLNVAKFIEAC--SNAGHLRYAYSVFT-HQPCPNTYLHNTMIRA 90

Query: 83  -----APPHHSISIFA---TWVSTSHFEFDDFTFIFVLGACARAPSVSTLMIGRQIHTHI 142
                 P  HSI+I      W   +  + D FTF FVL    R   VS +  GRQIH  +
Sbjct: 91  LSLLDEPNAHSIAITVYRKLWALCA--KPDTFTFPFVLKIAVR---VSDVWFGRQIHGQV 150

Query: 143 LKRGIVSNIWVQTTMIHFYSINKGVGSARKLFDEMSLRNSVTWNAMIAGY---------- 202
           +  G  S++ V T +I  Y    G+G ARK+FDEM +++   WNA++AGY          
Sbjct: 151 VVFGFDSSVHVVTGLIQMYFSCGGLGDARKMFDEMLVKDVNVWNALLAGYGKVGEMDEAR 210

Query: 203 -----------------CSQGGKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILS 262
                            C   G      A +A+E+F+ ML+E+    V+P + T++ +LS
Sbjct: 211 SLLEMMPCWVRNEVSWTCVISGYAKSGRASEAIEVFQRMLMEN----VEPDEVTLLAVLS 270

Query: 263 AASQLGMLETGSCVHAYIKKTVDSPEKDVFIGTGLVNMYSKCGLLNSASSVFKQMKQRNV 322
           A + LG LE G  + +Y+        + V +   +++MY+K G +  A  VF+ + +RNV
Sbjct: 271 ACADLGSLELGERICSYVDHR--GMNRAVSLNNAVIDMYAKSGNITKALDVFECVNERNV 330

Query: 323 LTWTSMATGLAVHGRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRV 382
           +TWT++  GLA HG G EAL + + M   GV+PN VTF ++LSAC H G ++ G  LF  
Sbjct: 331 VTWTTIIAGLATHGHGAEALAMFNRMVKAGVRPNDVTFIAILSACSHVGWVDLGKRLFNS 390

Query: 383 MERKFGVVPQMQHYGCIVDLLGRSGHLREAYKLILEMPMEPDGVLWRSLLSSCMLHGDVE 442
           M  K+G+ P ++HYGC++DLLGR+G LREA ++I  MP + +  +W SLL++  +H D+E
Sbjct: 391 MRSKYGIHPNIEHYGCMIDLLGRAGKLREADEVIKSMPFKANAAIWGSLLAASNVHHDLE 450

Query: 443 MGERVGKLLVERQGGESFDDEWCVGSEDFVALSNVYASAERWDDVEALRDEMKIKGIENK 475
           +GER    L++ +            S +++ L+N+Y++  RWD+   +R+ MK  G++  
Sbjct: 451 LGERALSELIKLEPN---------NSGNYMLLANLYSNLGRWDESRMMRNMMKGIGVKKM 494

BLAST of CSPI01G27250 vs. TAIR 10
Match: AT5G66520.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 273.9 bits (699), Expect = 2.6e-73
Identity = 164/510 (32.16%), Postives = 273/510 (53.53%), Query Frame = 0

Query: 2   HLAPRLSCIHHLSNVRISSLQLLQIHAQLITNGFKSPS-PYAKLITHLCKKSSSESIAHA 61
           +L   +SC+   S       +L QIHA+++  G    S    K ++     +SS+ + +A
Sbjct: 13  NLYETMSCLQRCSKQE----ELKQIHARMLKTGLMQDSYAITKFLSFCISSTSSDFLPYA 72

Query: 62  HLIFRHHRYSPNLFLFNTLIR---CA-PPHHSISIFATWVSTSHFEFDDFTFIFVLGACA 121
            ++F      P+ FL+N +IR   C+  P  S+ ++   + +S    + +TF  +L AC+
Sbjct: 73  QIVFDGFD-RPDTFLWNLMIRGFSCSDEPERSLLLYQRMLCSS-APHNAYTFPSLLKACS 132

Query: 122 RAPSVSTLMIGRQIHTHILKRGIVSNIWVQTTMIHFYSINKGVGSARKLFDEMSLRNSVT 181
              ++S      QIH  I K G  ++++   ++I+ Y++      A  LFD +   + V+
Sbjct: 133 ---NLSAFEETTQIHAQITKLGYENDVYAVNSLINSYAVTGNFKLAHLLFDRIPEPDDVS 192

Query: 182 WNAMIAGYCSQG-------------------------GKVSQKYARDALELFRGMLVEST 241
           WN++I GY   G                         G V     ++AL+LF     E  
Sbjct: 193 WNSVIKGYVKAGKMDIALTLFRKMAEKNAISWTTMISGYVQADMNKEALQLFH----EMQ 252

Query: 242 NFEVKPTDTTMVCILSAASQLGMLETGSCVHAYIKKTVDSPEKDVFIGTGLVNMYSKCGL 301
           N +V+P + ++   LSA +QLG LE G  +H+Y+ KT      D  +G  L++MY+KCG 
Sbjct: 253 NSDVEPDNVSLANALSACAQLGALEQGKWIHSYLNKT--RIRMDSVLGCVLIDMYAKCGE 312

Query: 302 LNSASSVFKQMKQRNVLTWTSMATGLAVHGRGKEALELLDAMGAHGVKPNAVTFTSLLSA 361
           +  A  VFK +K+++V  WT++ +G A HG G+EA+     M   G+KPN +TFT++L+A
Sbjct: 313 MEEALEVFKNIKKKSVQAWTALISGYAYHGHGREAISKFMEMQKMGIKPNVITFTAVLTA 372

Query: 362 CCHGGLIEEGLHLFRVMERKFGVVPQMQHYGCIVDLLGRSGHLREAYKLILEMPMEPDGV 421
           C + GL+EEG  +F  MER + + P ++HYGCIVDLLGR+G L EA + I EMP++P+ V
Sbjct: 373 CSYTGLVEEGKLIFYSMERDYNLKPTIEHYGCIVDLLGRAGLLDEAKRFIQEMPLKPNAV 432

Query: 422 LWRSLLSSCMLHGDVEMGERVGKLLV---ERQGGESFDDEWCVGSEDFVALSNVYASAER 479
           +W +LL +C +H ++E+GE +G++L+      GG             +V  +N++A  ++
Sbjct: 433 IWGALLKACRIHKNIELGEEIGEILIAIDPYHGGR------------YVHKANIHAMDKK 492

BLAST of CSPI01G27250 vs. TAIR 10
Match: AT5G59200.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 273.1 bits (697), Expect = 4.4e-73
Identity = 166/478 (34.73%), Postives = 259/478 (54.18%), Query Frame = 0

Query: 26  IHAQLITNGFKSPSPYAKLITHLCKKSSSESIAHAHLIFRHHRYSPNLFLFNTLI----R 85
           IHA++I       +     +  +C  S+ +S+ +A+ +F +   +PN++L+  +I     
Sbjct: 48  IHAKIIRTFHDQDAFVVFELIRVC--STLDSVDYAYDVFSYVS-NPNVYLYTAMIDGFVS 107

Query: 86  CAPPHHSISIFATWVSTSHFEFDDFTFIFVLGACARAPSVSTLMIGRQIHTHILKRGIVS 145
                  +S++   +  S    D++    VL AC        L + R+IH  +LK G  S
Sbjct: 108 SGRSADGVSLYHRMIHNSVLP-DNYVITSVLKAC-------DLKVCREIHAQVLKLGFGS 167

Query: 146 NIWVQTTMIHFYSINKGVGSARKLFDEMSLRNSVTWNAMIAGYCSQG------------- 205
           +  V   M+  Y  +  + +A+K+FDEM  R+ V    MI  Y   G             
Sbjct: 168 SRSVGLKMMEIYGKSGELVNAKKMFDEMPDRDHVAATVMINCYSECGFIKEALELFQDVK 227

Query: 206 ------------GKVSQKYARDALELFRGMLVESTNFEVKPTDTTMVCILSAASQLGMLE 265
                       G V  K    ALELFR M +E+    V   + T VC+LSA S LG LE
Sbjct: 228 IKDTVCWTAMIDGLVRNKEMNKALELFREMQMEN----VSANEFTAVCVLSACSDLGALE 287

Query: 266 TGSCVHAYIKKTVDSPEKDVFIGTGLVNMYSKCGLLNSASSVFKQMKQRNVLTWTSMATG 325
            G  VH++++      E   F+G  L+NMYS+CG +N A  VF+ M+ ++V+++ +M +G
Sbjct: 288 LGRWVHSFVEN--QRMELSNFVGNALINMYSRCGDINEARRVFRVMRDKDVISYNTMISG 347

Query: 326 LAVHGRGKEALELLDAMGAHGVKPNAVTFTSLLSACCHGGLIEEGLHLFRVMERKFGVVP 385
           LA+HG   EA+     M   G +PN VT  +LL+AC HGGL++ GL +F  M+R F V P
Sbjct: 348 LAMHGASVEAINEFRDMVNRGFRPNQVTLVALLNACSHGGLLDIGLEVFNSMKRVFNVEP 407

Query: 386 QMQHYGCIVDLLGRSGHLREAYKLILEMPMEPDGVLWRSLLSSCMLHGDVEMGERVGKLL 445
           Q++HYGCIVDLLGR G L EAY+ I  +P+EPD ++  +LLS+C +HG++E+GE++ K L
Sbjct: 408 QIEHYGCIVDLLGRVGRLEEAYRFIENIPIEPDHIMLGTLLSACKIHGNMELGEKIAKRL 467

Query: 446 VERQGGESFDDEWCVGSEDFVALSNVYASAERWDDVEALRDEMKIKGIENKAGCSSLQ 475
            E +  +         S  +V LSN+YAS+ +W +   +R+ M+  GIE + GCS+++
Sbjct: 468 FESENPD---------SGTYVLLSNLYASSGKWKESTEIRESMRDSGIEKEPGCSTIE 499

BLAST of CSPI01G27250 vs. TAIR 10
Match: AT5G48910.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 271.2 bits (692), Expect = 1.7e-72
Identity = 174/508 (34.25%), Postives = 261/508 (51.38%), Query Frame = 0

Query: 23  LLQIHAQLITNGFKSPSPYAKLITHLCKKSS--SESIAHAHLIFRHHRYSPNLFLFNTLI 82
           L QIHA  I +G    +  A  I   C  S      + +AH IF +     N F +NT+I
Sbjct: 39  LSQIHAVFIKSGQMRDTLAAAEILRFCATSDLHHRDLDYAHKIF-NQMPQRNCFSWNTII 98

Query: 83  RCAPPHH------SISIFATWVSTSHFEFDDFTFIFVLGACARAPSVSTLMIGRQIHTHI 142
           R            +I++F   +S    E + FTF  VL ACA+   +     G+QIH   
Sbjct: 99  RGFSESDEDKALIAITLFYEMMSDEFVEPNRFTFPSVLKACAKTGKIQE---GKQIHGLA 158

Query: 143 LKRGIVSNIWVQTTMIHFY----------------SINKGV------------------- 202
           LK G   + +V + ++  Y                 I K +                   
Sbjct: 159 LKYGFGGDEFVMSNLVRMYVMCGFMKDARVLFYKNIIEKDMVVMTDRRKRDGEIVLWNVM 218

Query: 203 ----------GSARKLFDEMSLRNSVTWNAMIAGYCSQGGKVSQKYARDALELFRGMLVE 262
                      +AR LFD+M  R+ V+WN MI+GY   G      + +DA+E+FR    E
Sbjct: 219 IDGYMRLGDCKAARMLFDKMRQRSVVSWNTMISGYSLNG------FFKDAVEVFR----E 278

Query: 263 STNFEVKPTDTTMVCILSAASQLGMLETGSCVHAYIKKTVDSPEKDVFIGTGLVNMYSKC 322
               +++P   T+V +L A S+LG LE G  +H Y + +      D  +G+ L++MYSKC
Sbjct: 279 MKKGDIRPNYVTLVSVLPAISRLGSLELGEWLHLYAEDS--GIRIDDVLGSALIDMYSKC 338

Query: 323 GLLNSASSVFKQMKQRNVLTWTSMATGLAVHGRGKEALELLDAMGAHGVKPNAVTFTSLL 382
           G++  A  VF+++ + NV+TW++M  G A+HG+  +A++    M   GV+P+ V + +LL
Sbjct: 339 GIIEKAIHVFERLPRENVITWSAMINGFAIHGQAGDAIDCFCKMRQAGVRPSDVAYINLL 398

Query: 383 SACCHGGLIEEGLHLFRVMERKFGVVPQMQHYGCIVDLLGRSGHLREAYKLILEMPMEPD 442
           +AC HGGL+EEG   F  M    G+ P+++HYGC+VDLLGRSG L EA + IL MP++PD
Sbjct: 399 TACSHGGLVEEGRRYFSQMVSVDGLEPRIEHYGCMVDLLGRSGLLDEAEEFILNMPIKPD 458

Query: 443 GVLWRSLLSSCMLHGDVEMGERVGKLLVERQGGESFDDEWCVGSEDFVALSNVYASAERW 478
            V+W++LL +C + G+VEMG+RV  +L++    +         S  +VALSN+YAS   W
Sbjct: 459 DVIWKALLGACRMQGNVEMGKRVANILMDMVPHD---------SGAYVALSNMYASQGNW 518

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LJ691.9e-12152.19Pentatricopeptide repeat-containing protein At3g18970 OS=Arabidopsis thaliana OX... [more]
Q9FMA11.6e-7234.29Pentatricopeptide repeat-containing protein At5g56310 OS=Arabidopsis thaliana OX... [more]
Q9FJY73.6e-7232.16Pentatricopeptide repeat-containing protein At5g66520 OS=Arabidopsis thaliana OX... [more]
Q9FIF76.1e-7234.73Putative pentatricopeptide repeat-containing protein At5g59200, chloroplastic OS... [more]
Q9FI802.3e-7134.25Pentatricopeptide repeat-containing protein At5g48910 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A0A0LZ633.2e-28199.18Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573660 PE=4 SV=1[more]
A0A5D3CFP07.3e-27095.06Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BPA57.3e-27095.06pentatricopeptide repeat-containing protein At3g18970 OS=Cucumis melo OX=3656 GN... [more]
A0A6J1JKN41.2e-22480.54pentatricopeptide repeat-containing protein At3g18970 OS=Cucurbita maxima OX=366... [more]
A0A6J1HCU64.8e-22179.71pentatricopeptide repeat-containing protein At3g18970 isoform X1 OS=Cucurbita mo... [more]
Match NameE-valueIdentityDescription
XP_011659935.16.6e-28199.18pentatricopeptide repeat-containing protein At3g18970 [Cucumis sativus][more]
XP_008450741.11.5e-26995.06PREDICTED: pentatricopeptide repeat-containing protein At3g18970 [Cucumis melo] ... [more]
XP_038877553.18.1e-24788.04pentatricopeptide repeat-containing protein At3g18970 [Benincasa hispida] >XP_03... [more]
XP_022988094.12.5e-22480.54pentatricopeptide repeat-containing protein At3g18970 [Cucurbita maxima][more]
XP_023515866.11.6e-22380.33pentatricopeptide repeat-containing protein At3g18970 [Cucurbita pepo subsp. pep... [more]
Match NameE-valueIdentityDescription
AT3G18970.11.3e-12252.19mitochondrial editing factor 20 [more]
AT5G56310.11.1e-7334.29Pentatricopeptide repeat (PPR) superfamily protein [more]
AT5G66520.12.6e-7332.16Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT5G59200.14.4e-7334.73Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT5G48910.11.7e-7234.25Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 285..334
e-value: 5.7E-11
score: 42.5
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 147..171
e-value: 0.65
score: 10.4
coord: 438..464
e-value: 0.4
score: 11.0
coord: 361..385
e-value: 0.086
score: 13.1
coord: 175..188
e-value: 0.036
score: 14.3
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 323..356
e-value: 2.2E-6
score: 25.5
coord: 289..322
e-value: 1.6E-4
score: 19.6
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 286..320
score: 10.972319
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 321..351
score: 10.248873
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 85..258
e-value: 3.4E-21
score: 77.9
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 306..480
e-value: 2.3E-26
score: 94.9
NoneNo IPR availablePANTHERPTHR47928:SF14PPR CONTAINING PLANT-LIKE PROTEINcoord: 13..478
NoneNo IPR availablePANTHERPTHR47928REPEAT-CONTAINING PROTEIN, PUTATIVE-RELATEDcoord: 13..478

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G27250.1CSPI01G27250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding