CSPI01G27150 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G27150
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Defensin-like family protein, putative
Location	Chr1: 22084997 .. 22085665 (+)
RNA-Seq Expression	CSPI01G27150
Synteny	CSPI01G27150

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CACACACTAAGAATTATTTCTACAATTCCACTAAAACAATGGCTAAAGTTGGACTTATTTCTTGTGTACTCATTGTCTTTCTGGTCTTTTATTCAGGTTTGTTTAACCTTTCTGCTTTAATTGAGTTCCATATCGTAATGATTTTCTTACCATCGTTTCAGTTTGTGACATTTTCCTGTAAATATCTTTTGACAAAAGAACTAATCGAGACGATATTGATAAATTCATTTTCTGATCGAAAGAAATTAAATTTACTAAAAACTCCCGGTGGGTGCACGGGGAATATGAATTCCCTACACCTTATACAAGTGCGCAAGAATACCACCGTTGGGATTTACTTACTTCCATCAAATGAACTGTGGGACTTATTTATTCAAATATATGGATATACTCTTTTATTTGATTCTATTAAAATTGTGGTACTAGCTATATATTATATTATGATGGTCACAGGAAATTTAAATGCAAATGCTGCTGTGTGCTGCAACAATAATCCAAGTTTAGGGAGCTGTAAGCCTGGAGTAGATGACAGTCCACAAGCTAATGGAAAGTGTTGGAGGTTATGCACTTCTGGATGTACAAAGGGAGGATTTTGCAAGCGAGTTGGGAGAAATGGCCGCCATGTCTGCCATTGTTATTGTTGATTAACTTCACCAATATACAATTACC

mRNA sequence

CACACACTAAGAATTATTTCTACAATTCCACTAAAACAATGGCTAAAGTTGGACTTATTTCTTGTGTACTCATTGTCTTTCTGGTCTTTTATTCAGGAAATTTAAATGCAAATGCTGCTGTGTGCTGCAACAATAATCCAAGTTTAGGGAGCTGTAAGCCTGGAGTAGATGACAGTCCACAAGCTAATGGAAAGTGTTGGAGGTTATGCACTTCTGGATGTACAAAGGGAGGATTTTGCAAGCGAGTTGGGAGAAATGGCCGCCATGTCTGCCATTGTTATTGTTGATTAACTTCACCAATATACAATTACC

Coding sequence (CDS)

ATGGCTAAAGTTGGACTTATTTCTTGTGTACTCATTGTCTTTCTGGTCTTTTATTCAGGAAATTTAAATGCAAATGCTGCTGTGTGCTGCAACAATAATCCAAGTTTAGGGAGCTGTAAGCCTGGAGTAGATGACAGTCCACAAGCTAATGGAAAGTGTTGGAGGTTATGCACTTCTGGATGTACAAAGGGAGGATTTTGCAAGCGAGTTGGGAGAAATGGCCGCCATGTCTGCCATTGTTATTGTTGA

Protein sequence

MAKVGLISCVLIVFLVFYSGNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTSGCTKGGFCKRVGRNGRHVCHCYC*

Homology

[Close]

BLAST of CSPI01G27150 vs. ExPASy Swiss-Prot
Match: P0CAX9 (Defensin-like protein 21 OS=Arabidopsis thaliana OX=3702 GN=At4g14276 PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 4.9e-14
Identity = 34/94 (36.17%), Postives = 50/94 (53.19%), Query Frame = 0


Query: 1  MAKVGLISCVLIVFLVFYSGNLNANA------------AVCCNNNPSLGSCKPGVDDSPQ 60
          M +  ++S VL   +V   G++  +             ++CC  +PS+G C P +DDS +
Sbjct: 1  MVRTNVVSFVLFAAIVLCIGSIQIDGQKHTAPWIPEDNSICCKEHPSVGRCLPNIDDSAE 60

Query: 61 ANGKCWRLCTSGCTKGGFCKRVGRNGRHVCHCYC 83
            GKCW+ C  GC  GGFCK  G   +H+CHC C
Sbjct: 61 KGGKCWKFCIEGCETGGFCKLFGH--KHICHCNC 92

[Close]

BLAST of CSPI01G27150 vs. ExPASy Swiss-Prot
Match: Q2V2S1 (Putative defensin-like protein 20 OS=Arabidopsis thaliana OX=3702 GN=At5g52605 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 4.2e-13
Identity = 34/90 (37.78%), Postives = 50/90 (55.56%), Query Frame = 0


Query: 1  MAKVGLISCVL---IVFLVFYSGN-----LNANAAVCCNNNPSLGSCKPGVDDSPQANGK 60
          M +  ++S VL   IV  + Y        +  + ++CC  +PS+G C PG+DD  + +GK
Sbjct: 1  MVRTNVVSFVLFAAIVLCILYGQKHIAPWIFEDKSICCKEHPSVGRCLPGIDDDAEKDGK 60

Query: 61 CWRLCTSGCTKGGFCKRVGRNGRHVCHCYC 83
          CW+ C  GC  GGFCK      +H+CHC C
Sbjct: 61 CWKFCIEGCETGGFCKLF--EHKHICHCNC 88

[Close]

BLAST of CSPI01G27150 vs. ExPASy Swiss-Prot
Match: Q2L6T3 (Putative defensin-like protein 27 OS=Arabidopsis thaliana OX=3702 GN=At4g18823 PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.3e-06
Identity = 24/60 (40.00%), Postives = 31/60 (51.67%), Query Frame = 0


Query: 23 NANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTSGCTKGGFCKRVGRNGRHVCHCYC 82
          N   ++CCN++P  G C          N  C + C  GCT+GGFCK      R +CHCYC
Sbjct: 30 NKGDSLCCNDHPEFGIC---------TNKSCNKWCLQGCTRGGFCK------RKICHCYC 74

[Close]

BLAST of CSPI01G27150 vs. ExPASy TrEMBL
Match: A0A0A0LZ58 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573070 PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.2e-42
Identity = 81/82 (98.78%), Postives = 82/82 (100.00%), Query Frame = 0


Query: 1  MAKVGLISCVLIVFLVFYSGNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTSG 60
          MAKVGLISCVL+VFLVFYSGNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTSG
Sbjct: 1  MAKVGLISCVLVVFLVFYSGNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTSG 60

Query: 61 CTKGGFCKRVGRNGRHVCHCYC 83
          CTKGGFCKRVGRNGRHVCHCYC
Sbjct: 61 CTKGGFCKRVGRNGRHVCHCYC 82

[Close]

BLAST of CSPI01G27150 vs. ExPASy TrEMBL
Match: A0A0A0LW61 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573050 PE=3 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 6.4e-33
Identity = 63/64 (98.44%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 19 SGNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTSGCTKGGFCKRVGRNGRHVC 78
          +GNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTSGCTKGGFCKRVGRNGRHVC
Sbjct: 4  TGNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTSGCTKGGFCKRVGRNGRHVC 63

Query: 79 HCYC 83
          HCYC
Sbjct: 64 HCYC 67

[Close]

BLAST of CSPI01G27150 vs. ExPASy TrEMBL
Match: A0A0A0LYL5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573040 PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.9e-21
Identity = 53/83 (63.86%), Postives = 63/83 (75.90%), Query Frame = 0


Query: 1  MAKVGLIS-CVLIVFLVFYSGNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTS 60
          MAKVG+IS  V IVFL FYSG+LN NA +CC N+P  G+CKPGVDD+   +G+CW  C  
Sbjct: 1  MAKVGVISWVVFIVFLAFYSGSLNTNAQLCCINHPEWGACKPGVDDA--TDGRCWLKCIG 60

Query: 61 GCTKGGFCKRVGRNGRHVCHCYC 83
          GC KGG CKRVG+  +HVCHCYC
Sbjct: 61 GCEKGGICKRVGK--KHVCHCYC 79

[Close]

BLAST of CSPI01G27150 vs. ExPASy TrEMBL
Match: A0A5D3CFQ3 (Putative defensin-like protein 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003350 PE=3 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 4.1e-19
Identity = 49/83 (59.04%), Postives = 62/83 (74.70%), Query Frame = 0


Query: 1  MAKVGLI-SCVLIVFLVFYSGNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTS 60
          MAK+G I   V I FLV YSG+ N NA +CCNN+P LGSC+PGVDD+  ++GKCW  C +
Sbjct: 1  MAKLGAIFGWVFIAFLVLYSGSKNTNAQLCCNNHPELGSCEPGVDDA--SDGKCWLHCIA 60

Query: 61 GCTKGGFCKRVGRNGRHVCHCYC 83
          GC KGGFCK++    ++VCHCYC
Sbjct: 61 GCEKGGFCKQL--TDKNVCHCYC 79

[Close]

BLAST of CSPI01G27150 vs. ExPASy TrEMBL
Match: A0A0A0LWJ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573030 PE=3 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.3e-17
Identity = 47/83 (56.63%), Postives = 58/83 (69.88%), Query Frame = 0


Query: 1  MAKVGLI-SCVLIVFLVFYSGNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTS 60
          MAK+G I S V + FLV Y+G++N NA +CCNN+  LGSC PGVDD    +GKCW  C  
Sbjct: 1  MAKLGAIFSWVFVAFLVLYAGSMNTNAQLCCNNHRELGSCVPGVDD--DYDGKCWHHCIV 60

Query: 61 GCTKGGFCKRVGRNGRHVCHCYC 83
          GC +GGFCK++     HVCHCYC
Sbjct: 61 GCERGGFCKKLWYG--HVCHCYC 79

[Close]

BLAST of CSPI01G27150 vs. NCBI nr
Match: KAE8653392.1 (hypothetical protein Csa_007412 [Cucumis sativus])

HSP 1 Score: 149.1 bits (375), Expect = 1.7e-32
Identity = 78/125 (62.40%), Postives = 79/125 (63.20%), Query Frame = 0


Query: 1   MAKVGLIS-CVLIVFLVFYS---------------------------------------- 60
           MAKVG+IS  V IVFL FYS                                        
Sbjct: 1   MAKVGVISWVVFIVFLAFYSGSLNTNAQLCCINHPEWGACKPGVDDATDGRCWLKCIGGC 60

Query: 61  --GNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTSGCTKGGFCKRVGRNGRHV 83
             GNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTSGCTKGGFCKRVGRNGRHV
Sbjct: 61  EKGNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTSGCTKGGFCKRVGRNGRHV 120

[Close]

BLAST of CSPI01G27150 vs. NCBI nr
Match: TYK10190.1 (putative defensin-like protein 20 [Cucumis melo var. makuwa])

HSP 1 Score: 103.6 bits (257), Expect = 8.4e-19
Identity = 49/83 (59.04%), Postives = 62/83 (74.70%), Query Frame = 0


Query: 1  MAKVGLI-SCVLIVFLVFYSGNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTS 60
          MAK+G I   V I FLV YSG+ N NA +CCNN+P LGSC+PGVDD+  ++GKCW  C +
Sbjct: 1  MAKLGAIFGWVFIAFLVLYSGSKNTNAQLCCNNHPELGSCEPGVDDA--SDGKCWLHCIA 60

Query: 61 GCTKGGFCKRVGRNGRHVCHCYC 83
          GC KGGFCK++    ++VCHCYC
Sbjct: 61 GCEKGGFCKQL--TDKNVCHCYC 79

[Close]

BLAST of CSPI01G27150 vs. NCBI nr
Match: KGN66133.1 (hypothetical protein Csa_007601 [Cucumis sativus])

HSP 1 Score: 98.6 bits (244), Expect = 2.7e-17
Identity = 47/83 (56.63%), Postives = 58/83 (69.88%), Query Frame = 0


Query: 1  MAKVGLI-SCVLIVFLVFYSGNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTS 60
          MAK+G I S V + FLV Y+G++N NA +CCNN+  LGSC PGVDD    +GKCW  C  
Sbjct: 1  MAKLGAIFSWVFVAFLVLYAGSMNTNAQLCCNNHRELGSCVPGVDD--DYDGKCWHHCIV 60

Query: 61 GCTKGGFCKRVGRNGRHVCHCYC 83
          GC +GGFCK++     HVCHCYC
Sbjct: 61 GCERGGFCKKLWYG--HVCHCYC 79

[Close]

BLAST of CSPI01G27150 vs. NCBI nr
Match: KGN66136.1 (hypothetical protein Csa_007074 [Cucumis sativus])

HSP 1 Score: 98.2 bits (243), Expect = 3.5e-17
Identity = 46/83 (55.42%), Postives = 59/83 (71.08%), Query Frame = 0


Query: 1  MAKVG-LISCVLIVFLVFYSGNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTS 60
          MAK+G     V I FLV YSG++N NA +CCNN+P LG C+ GVDD+   +GKCW  C +
Sbjct: 1  MAKLGATFGLVFIAFLVLYSGSMNTNAQLCCNNHPELGECEAGVDDA--NDGKCWLHCIA 60

Query: 61 GCTKGGFCKRVGRNGRHVCHCYC 83
          GC KGGFCK +  + ++VCHCYC
Sbjct: 61 GCEKGGFCKHL--SDKNVCHCYC 79

[Close]

BLAST of CSPI01G27150 vs. NCBI nr
Match: EOX93667.1 (Defensin-like family protein, putative [Theobroma cacao])

HSP 1 Score: 97.4 bits (241), Expect = 6.0e-17
Identity = 43/77 (55.84%), Postives = 52/77 (67.53%), Query Frame = 0


Query: 6  LISCVLIVFLVFYSGNLNANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTSGCTKGG 65
          L+   LI+F+ F    + A  A CCNN+P LG C PG DD PQ  GKCW  C+SGC KGG
Sbjct: 10 LLLAALILFIDFGQVQVVAAQARCCNNHPDLGRCLPGQDDDPQKGGKCWNYCSSGCQKGG 69

Query: 66 FCKRVGRNGRHVCHCYC 83
          FCKR+ ++G H CHCYC
Sbjct: 70 FCKRM-KDGHHECHCYC 85

[Close]

BLAST of CSPI01G27150 vs. TAIR 10
Match: AT4G14276.1 (Defensin-like (DEFL) family protein )

HSP 1 Score: 78.2 bits (191), Expect = 3.5e-15
Identity = 34/94 (36.17%), Postives = 50/94 (53.19%), Query Frame = 0


Query: 1  MAKVGLISCVLIVFLVFYSGNLNANA------------AVCCNNNPSLGSCKPGVDDSPQ 60
          M +  ++S VL   +V   G++  +             ++CC  +PS+G C P +DDS +
Sbjct: 1  MVRTNVVSFVLFAAIVLCIGSIQIDGQKHTAPWIPEDNSICCKEHPSVGRCLPNIDDSAE 60

Query: 61 ANGKCWRLCTSGCTKGGFCKRVGRNGRHVCHCYC 83
            GKCW+ C  GC  GGFCK  G   +H+CHC C
Sbjct: 61 KGGKCWKFCIEGCETGGFCKLFGH--KHICHCNC 92

[Close]

BLAST of CSPI01G27150 vs. TAIR 10
Match: AT5G52605.1 (Defensin-like (DEFL) family protein )

HSP 1 Score: 75.1 bits (183), Expect = 3.0e-14
Identity = 34/90 (37.78%), Postives = 50/90 (55.56%), Query Frame = 0


Query: 1  MAKVGLISCVL---IVFLVFYSGN-----LNANAAVCCNNNPSLGSCKPGVDDSPQANGK 60
          M +  ++S VL   IV  + Y        +  + ++CC  +PS+G C PG+DD  + +GK
Sbjct: 1  MVRTNVVSFVLFAAIVLCILYGQKHIAPWIFEDKSICCKEHPSVGRCLPGIDDDAEKDGK 60

Query: 61 CWRLCTSGCTKGGFCKRVGRNGRHVCHCYC 83
          CW+ C  GC  GGFCK      +H+CHC C
Sbjct: 61 CWKFCIEGCETGGFCKLF--EHKHICHCNC 88

[Close]

BLAST of CSPI01G27150 vs. TAIR 10
Match: AT4G18823.1 (Defensin-like (DEFL) family protein )

HSP 1 Score: 53.5 bits (127), Expect = 9.3e-08
Identity = 24/60 (40.00%), Postives = 31/60 (51.67%), Query Frame = 0


Query: 23 NANAAVCCNNNPSLGSCKPGVDDSPQANGKCWRLCTSGCTKGGFCKRVGRNGRHVCHCYC 82
          N   ++CCN++P  G C          N  C + C  GCT+GGFCK      R +CHCYC
Sbjct: 30 NKGDSLCCNDHPEFGIC---------TNKSCNKWCLQGCTRGGFCK------RKICHCYC 74

The following BLAST results are available for this feature:

BLAST of CSPI01G27150 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0CAX9	4.9e-14	36.17	Defensin-like protein 21 OS=Arabidopsis thaliana OX=3702 GN=At4g14276 PE=2 SV=1	[more]
Q2V2S1	4.2e-13	37.78	Putative defensin-like protein 20 OS=Arabidopsis thaliana OX=3702 GN=At5g52605 P...	[more]
Q2L6T3	1.3e-06	40.00	Putative defensin-like protein 27 OS=Arabidopsis thaliana OX=3702 GN=At4g18823 P...	[more]

BLAST of CSPI01G27150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LZ58	1.2e-42	98.78	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573070 PE=3 SV=1	[more]
A0A0A0LW61	6.4e-33	98.44	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573050 PE=3 SV=1	[more]
A0A0A0LYL5	1.9e-21	63.86	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573040 PE=3 SV=1	[more]
A0A5D3CFQ3	4.1e-19	59.04	Putative defensin-like protein 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A0A0LWJ9	1.3e-17	56.63	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573030 PE=3 SV=1	[more]

BLAST of CSPI01G27150 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8653392.1	1.7e-32	62.40	hypothetical protein Csa_007412 [Cucumis sativus]	[more]
TYK10190.1	8.4e-19	59.04	putative defensin-like protein 20 [Cucumis melo var. makuwa]	[more]
KGN66133.1	2.7e-17	56.63	hypothetical protein Csa_007601 [Cucumis sativus]	[more]
KGN66136.1	3.5e-17	55.42	hypothetical protein Csa_007074 [Cucumis sativus]	[more]
EOX93667.1	6.0e-17	55.84	Defensin-like family protein, putative [Theobroma cacao]	[more]

BLAST of CSPI01G27150 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G14276.1	3.5e-15	36.17	Defensin-like (DEFL) family protein	[more]
AT5G52605.1	3.0e-14	37.78	Defensin-like (DEFL) family protein	[more]
AT4G18823.1	9.3e-08	40.00	Defensin-like (DEFL) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR022618	Defensin-like protein 20-27	PFAM	PF10868	Defensin_like	coord: 29..82 e-value: 7.8E-13 score: 49.0
IPR022618	Defensin-like protein 20-27	PANTHER	PTHR34453	DEFENSIN-LIKE (DEFL) FAMILY PROTEIN-RELATED	coord: 6..82
None	No IPR available	PANTHER	PTHR34453:SF3	DEFENSIN-LIKE (DEFL) FAMILY PROTEIN-RELATED	coord: 6..82

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G27150.1	CSPI01G27150.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050832	defense response to fungus
cellular_component	GO:0005576	extracellular region